Rybp cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rybp
Entrez Gene ID	56353
Full Name	RING1 and YY1 binding protein
Synonyms	2410018J24Rik,DEDAF,YEAF1
General protein information	Preferred Names RING1 and YY1 binding protein Names RING1 and YY1-binding protein DED-associated factor Ring1 interactor Rybp death effector domain-associated factor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_019743.3	NP_062717.2	RING1 and YY1-binding protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	RYBP	NP_036366.3
Pan troglodytes (chimpanzee)	RYBP	XP_516581.2
Canis lupus familiaris (dog)	RYBP	XP_005632351.1
Danio rerio (zebrafish)	rybpb	NP_001012373.1
Danio rerio (zebrafish)	rybpa	NP_958474.1
Mus musculus (house mouse)	Rybp	NP_062717.2
Mus musculus (house mouse)	Gm6910	XP_898612.4
Gallus gallus (chicken)	RYBP	XP_414428.4

Gene Ontology
Bibliography

Related articles in PubMed

RNA Helicase DDX5 Inhibits Reprogramming to Pluripotency by miRNA-Based Repression of RYBP and its PRC1-Dependent and -Independent Functions.
Li H, Lai P, Jia J, Song Y, Xia Q, Huang K, He N, Ping W, Chen J, Yang Z, Li J, Yao M, Dong X, Zhao J, Hou C, Esteban MA, Gao S, Pei D, Hutchins AP, Yao H
Cell stem cell20(4)462-477.e6(2017 Apr)

RYBP stimulates PRC1 to shape chromatin-based communication between Polycomb repressive complexes.
Rose NR, King HW, Blackledge NP, Fursova NA, Ember KJ, Fischer R, Kessler BM, Klose RJ
eLife5(2016 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these results reveal important functions of DDX5 in regulating reprogramming and highlight the importance of a Ddx5-miR125b-Rybp axis in controlling cell fate.
Title: RNA Helicase DDX5 Inhibits Reprogramming to Pluripotency by miRNA-Based Repression of RYBP and its PRC1-Dependent and -Independent Functions.

Here, the authors discover that the histone H2AK119 E3 ubiquitin ligase activity of Polycomb repressive complex 1 (PRC1) is defined by the composition of its catalytic subunits and is highly regulated by RYBP/YAF2-dependent stimulation.
Title: RYBP stimulates PRC1 to shape chromatin-based communication between Polycomb repressive complexes.

These results show that a sustained regulation of the B-1-to-B-2 B cell lineage switch is needed throughout adult life and that RYBP plays an important role in keeping B-2 dominance, most likely independently of its Polycomb affiliation.
Title: Role of Polycomb RYBP in Maintaining the B-1-to-B-2 B-Cell Lineage Switch in Adult Hematopoiesis.

The data provide evidence for a previously unrecognized function of Rybp in heart development and point out the importance of germ cell lineage gene silencing during somatic differentiation.
Title: Lack of Rybp in Mouse Embryonic Stem Cells Impairs Cardiac Differentiation.

Although the genomic localization of the Cbx7- and RYBP-containing PRC1 complexes overlaps in certain genes, it can also be mutually exclusive.
Title: RYBP and Cbx7 define specific biological functions of polycomb complexes in mouse embryonic stem cells.

The following Rybp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rybp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu11359	NM_019743.3 Latest version!	Mus musculus RING1 and YY1 binding protein (Rybp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rybp ( NM_019743.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062717.2 Mus musculus RING1 and YY1 binding protein (Rybp), mRNA.

Price & Availability↑

CloneID	OMu11359
Clone ID Related Accession (Same CDS sequence)	NM_019743.3
Accession Version	NM_019743.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	RING1 and YY1-binding protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC158655.4. On Mar 21, 2007 this sequence version replaced NM_019743.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC053016.1, BC080287.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGACCATGG GCGACAAGAA GAGCCCGACC AGGCCAAAAA GACAAGCGAA ACCTGCCGCA 
GACGAAGGCT TTTGGGATTG TAGCGTCTGC ACCTTTAGGA ACAGCGCCGA AGCCTTTAAA 
TGCAGCATCT GCGATGTGCG GAAAGGCACC TCCACCAGGA AACCTCGCAT CAATTCTCAG 
CTGGTGGCAC AGCAGGTGGC ACAGCAGTAC GCCACTCCAC CTCCCCCTAA GAAGGAGAAG 
AAGGAGAAGG TCGAAAAGCC TGACAAAGAA AAGCCAGAGA AAGACAAGGA CATTAGCCCC 
AGTGTCACCA AGAAAAACAC CAACAAGAAA ACAAAACCAA AGTCTGATAT TCTGAAAGAT 
CCTCCTAGTG AAGCTAACAG CATACAGTCT GCTAACGCTA CAACAAAGAC CAGCGAAACA 
AACCACACCT CAAGGCCCCG GCTGAAGAAT GTGGACAGGA GCACCGCACA GCAGTTGGCA 
GTAACTGTGG GCAACGTCAC CGTCATTATC ACAGACTTTA AGGAAAAGAC TCGCTCCTCC 
TCCACATCCT CTTCCACAGT GACCTCCAGT GCAGGGTCAG AACAGCAGAA CCAGAGCAGC 
TCGGGCTCAG AGAGCACAGA CAAAGGCTCC TCCCGCTCCT CCACGCCAAA GGGCGACATG 
TCAGCAGTGA ATGATGAATC TTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062717.2
CDS	82..768
Translation	MTMGDKKSPTRPKRQAKPAADEGFWDCSVCTFRNSAEAFKCSICDVRKGTSTRKPRINSQLVAQQVAQQYATPPPPKKEKKEKVEKPDKEKPEKDKDISPSVTKKNTNKKTKPKSDILKDPPSEANSIQSANATTKTSETNHTSRPRLKNVDRSTAQQLAVTVGNVTVIITDFKEKTRSSSTSSSTVTSSAGSEQQNQSSSGSESTDKGSSRSSTPKGDMSAVNDESF

Target ORF information:

RefSeq Version	NM_019743.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus RING1 and YY1 binding protein (Rybp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019743.3

ORF Insert Sequence:

ATGACCATGG GCGACAAGAA GAGCCCGACC AGGCCAAAAA GACAAGCGAA ACCTGCCGCA 
GACGAAGGCT TTTGGGATTG TAGCGTCTGC ACCTTTAGGA ACAGCGCCGA AGCCTTTAAA 
TGCAGCATCT GCGATGTGCG GAAAGGCACC TCCACCAGGA AACCTCGCAT CAATTCTCAG 
CTGGTGGCAC AGCAGGTGGC ACAGCAGTAC GCCACTCCAC CTCCCCCTAA GAAGGAGAAG 
AAGGAGAAGG TCGAAAAGCC TGACAAAGAA AAGCCAGAGA AAGACAAGGA CATTAGCCCC 
AGTGTCACCA AGAAAAACAC CAACAAGAAA ACAAAACCAA AGTCTGATAT TCTGAAAGAT 
CCTCCTAGTG AAGCTAACAG CATACAGTCT GCTAACGCTA CAACAAAGAC CAGCGAAACA 
AACCACACCT CAAGGCCCCG GCTGAAGAAT GTGGACAGGA GCACCGCACA GCAGTTGGCA 
GTAACTGTGG GCAACGTCAC CGTCATTATC ACAGACTTTA AGGAAAAGAC TCGCTCCTCC 
TCCACATCCT CTTCCACAGT GACCTCCAGT GCAGGGTCAG AACAGCAGAA CCAGAGCAGC 
TCGGGCTCAG AGAGCACAGA CAAAGGCTCC TCCCGCTCCT CCACGCCAAA GGGCGACATG 
TCAGCAGTGA ATGATGAATC TTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RNA Helicase DDX5 Inhibits Reprogramming to Pluripotency by miRNA-Based Repression of RYBP and its PRC1-Dependent and -Independent Functions.
Cell stem cell20(4)462-477.e6(2017 Apr)
Li H,Lai P,Jia J,Song Y,Xia Q,Huang K,He N,Ping W,Chen J,Yang Z,Li J,Yao M,Dong X,Zhao J,Hou C,Esteban MA,Gao S,Pei D,Hutchins AP,Yao H

RYBP stimulates PRC1 to shape chromatin-based communication between Polycomb repressive complexes.
eLife5(2016 10)
Rose NR,King HW,Blackledge NP,Fursova NA,Ember KJ,Fischer R,Kessler BM,Klose RJ