Astn2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Astn2
Entrez Gene ID	56079
Full Name	astrotactin 2
Synonyms	1d8,Astnl,bM452J22.1
General protein information	Preferred Names astrotactin 2 Names astrotactin-2 bM243L4.1 (astrotactin 2) bM264P11.1 (astrotactin 2) bM383P24.1 (astrotactin 2) bM423P8.1 (astrotactin 2) bM452J22.1 (novel protein) bM468K2.1 (astrotactin 2)
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 C1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006538104.3	XP_006538167.1	astrotactin-2 isoform X1
XM_030253680.1	XP_030109540.1	astrotactin-2 isoform X2
XM_006538105.4	XP_006538168.1	astrotactin-2 isoform X3
NM_207109.2	NP_996992.1	astrotactin-2 isoform b precursor
XM_006538105.3	XP_006538168.1	astrotactin-2 isoform X2
NM_019514.3	NP_062387.2	astrotactin-2 isoform a precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ASTN2	XP_002690000.3
Mus musculus (house mouse)	Astn2	NP_996992.1
Gallus gallus (chicken)	ASTN2	XP_003642322.2
	ASTN2	XP_001098403.1
Canis lupus familiaris (dog)	ASTN2	XP_005627020.1
Xenopus tropicalis (tropical clawed frog)	astn2	XP_002935251.2
Rattus norvegicus (Norway rat)	Astn2	NP_001258292.1
Homo sapiens (human)	ASTN2	NP_054729.3

Gene Ontology
Bibliography

Related articles in PubMed

Intramembrane Proteolysis of Astrotactins.
Chang H, Smallwood PM, Williams J, Nathans J
The Journal of biological chemistry292(8)3506-3516(2017 02)

A dual-strategy expression screen for candidate connectivity labels in the developing thalamus.
Bibollet-Bahena O, Okafuji T, Hokamp K, Tear G, Mitchell KJ
PloS one12(5)e0177977(2017)

Regulation of Tlx3 by Pax6 is required for the restricted expression of Chrn?3 in Cerebellar Granule Neuron progenitors during development.
Divya TS, Lalitha S, Parvathy S, Subashini C, Sanalkumar R, Dhanesh SB, Rasheed VA, Divya MS, Tole S, James J
Scientific reports630337(2016 Jul)

Identification of Astrotactin2 as a Genetic Modifier That Regulates the Global Orientation of Mammalian Hair Follicles.
Chang H, Cahill H, Smallwood PM, Wang Y, Nathans J
PLoS genetics11(9)e1005532(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study has defined the transmembrane topology of mouse Astn2 and shown that this protein undergoes a single intramembrane proteolytic cleavage in the second of two transmembrane segments.
Title: Intramembrane Proteolysis of Astrotactins.

These experiments suggest that Astn2, an endosomal membrane protein, modulates Planar cell polarity signaling.
Title: Identification of Astrotactin2 as a Genetic Modifier That Regulates the Global Orientation of Mammalian Hair Follicles.

ASTN2 regulates the levels of ASTN1 in the plasma membrane and that the release of neuronal adhesions to the glial fiber during neuronal locomotion involves the intracellular trafficking of ASTN1.
Title: Astn2, a novel member of the astrotactin gene family, regulates the trafficking of ASTN1 during glial-guided neuronal migration.

The following Astn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Astn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu33058	XM_006538105.4 Latest version!	Mus musculus astrotactin 2 (Astn2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu88592	XM_030253680.1 Latest version!	Mus musculus astrotactin 2 (Astn2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu13426	NM_019514.3 Latest version!	Mus musculus astrotactin 2 (Astn2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$762.30 $1089.00
OMu13528	NM_207109.2 Latest version!	Mus musculus astrotactin 2 (Astn2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$790.30 $1129.00
OMu33057	XM_006538104.3 Latest version!	Mus musculus astrotactin 2 (Astn2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
View Old Accession Versions >>
OMu33058	XM_006538105.3	Mus musculus astrotactin 2 (Astn2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Astn2 ( XM_006538105.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538168.1 Mus musculus astrotactin 2 (Astn2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu33058
Clone ID Related Accession (Same CDS sequence)	XM_006538105.4 , XM_006538105.3
Accession Version	XM_006538105.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	astrotactin-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006538105.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCG CCGGCGCCCG GCGCAGCCCG GGCCGCGGCT TGGGGCTTCG GGGGCGGCCG 
AGGCTCGGCT TCCACCCGGG GCCGCCGCCG CCACCGCCGC CGCCGCTGCT GTTGCTGTTT 
CTGCTCCTGC TGCCGCCGCC GCCGTTGCTG GCCGGCGCCA CGGCCGCCGC CGCCTCGCGG 
GAGCCCGACA GCCCATGCCG GCTCAAGACC GTCACCGTAT CCACGCTGCC CGCTCTGCGG 
GAAAGCGACA TCGGCTGGAG CGGTGCCCGC ACCGGGGCCG CGGCCGGGGC TGGGGCCGGG 
ACCGGAGCCG GGGCCGGAGC TGCTGCTGCT GCCGCCTCCG CCGCGTCCCC GGGCTCCGCG 
GGTTCAGCCG GCACAGCCGC GGAGTCTCGT CTCCTTCTCT TTGTGCGTAA TGAGCTCCCC 
GGGCGCATCG CGGTACAAGA TGACCTGGAC AACACTGAGC TGCCCTTCTT CACCCTGGAG 
ATGTCTGGCA CAGCAGCGGA CATTTCTTTG GTTCACTGGA GGCAGCAGTG GCTGGAGAAT 
GGCACATTGT ACTTCCACGT TTCCATGAGC AGCTCTGGGC AACTGGCTCA GGCCACTGCT 
CCCACACTCC AGGAGCCCTC GGAGATCGTT GAGGAACAGA TGCATATCCT CCACATTTCT 
GTGATGGGTG GACTCATTGC ACTTCTCCTC CTGCTGTTGG TGTTCACAGT GGCACTGTAT 
GCCCAGCGAC GCTGGCAGAA GCGCCGGCGC ATCCCACAGA AGAGCGCAAG TACAGAAGCT 
ACTCATGAGA TTCACTACAT CCCATCAGTG CTGCTGGGTC CTCAGGCCAG GGAAAGCTTC 
CGATCCTCCA GACTTCAGAC ACACAACTCA GTCATTGGTG TGCCTATCCG GGAAACCCCC 
ATCCTGGATG ACTATGACTA TGAAGAGGAG GAGGAACCAC CCAGGCGAGC CAACCACGTC 
TCCCGTGAGG ATGAGTTTGG TAGCCAGATG ACCCATGCCC TGGACAGCTT GGGAAGGCCA 
GGAGAAGAGA AAGTGGAATT TGAGAAGAAA GCAGCAGCTG AGGCGACACA GGAAACAGTG 
GAGTCCCTGA TGCAGAAGTT CAAGGAGAGT TTCCGTGCTA ACACACCAGT GGAGATCGGT 
CAGTTGCAGC CAGCCTCGCG CAGCAGCACC TCTGCAGGGA AGAGGAAGCG GAGGAACAAA 
TCTCGAGGGG GAATCAGCTT TGGGAGAACC AAGGGGACAT CAGGCTCAGA AGCAGATGAC 
GAAACACAGC TGACCTTCTA CACAGAGCAG TACCGCAGTC GCCGCCGCAG CAAAGGTTTA 
CTGAAAAGCC CTGTGAATAA GACAGCCTTA ACACTGATTG CTGTGAGCTC CTGCATCTTG 
GCCATGGTGT GTGGCAACCA GATGTCCTGT CCACTTACTG TGAAGGTGAC TTTGCATGTG 
CCTGAACACT TCATTGCAGA TGGGAGCAGC TTTGTGGTGA GTGAAGGAAG CTACCTGGAT 
ATCTCCGATT GGCTAAACCC TGCCAAGCTG TCTCTGTATT ACCAGATCAA TGCCACCTCG 
CCATGGGTGA GAGACCTCTG TGGACAGAGG ACGACAGATG CCTGTGAACA GCTCTGTGAC 
CCAGATACTG GGGAGTGCAG TTGTCATGAA GGTTACGCCC CTGACCCAGT CCACAGACAT 
CTATGTGTTC GCAGTGACTG GGGTCAAAGT GAAGGACCTT GGCCTTACAC AACACTGGAG 
AGGGGCTATG ATCTGGTGAC AGGAGAGCAA GCCCCTGAAA AGATTCTCAG GTCTACTTTC 
AGCTTGGGCC AAGGCCTCTG GCTTCCGGTC AGCAAAAGCT TTGTGGTGCC ACCGGTGGAG 
CTGTCCATCA ACCCCCTGGC CAGCTGCAAG ACGGATGTGC TCGTCACAGA AGACCCTGCG 
GATGTCAGGG AAGAAGCAAT GTTGTCCACG TACTTTGAAA CCATCAACGA CCTGCTGTCC 
TCCTTTGGTC CAGTCCGTGA CTGCTCAAGG AACAACGGGG GCTGTACCCG TAACTTCAAG 
TGTGTGTCTG ACCGCCAGGT GGACTCCTCG GGATGTGTGT GTCCTGAGGA ACTGAAGCCC 
ATGAAGGATG GCTCTGGCTG CTATGATCAC TCCAAGGGCA TCGACTGCTC TGATGGCTTT 
AATGGAGGGT GTGAGCAGCT TTGCCTACAA CAAACACTGC CTTTGCCCTA TGATACCACC 
TCCAGCACCA TCTTCATGTT CTGCGGCTGT GTGGAAGAGT ACAAGCTGGC TCCTGATGGG 
AAATCCTGTC TAATGCTCTC AGATGTCTGT GAGGGCCCCA AGTGCCTCAA ACCTGACTCC 
AAATTCAATG ACACCCTCTT TGGAGAGATG CTACATGGTT ACAACAACCG GACACAACAT 
GTAAACCAAG GCCAAGTCTT CCAAATGACA TTCAGGGAGA ATAACTTCAT CAAGGACTTT 
CCACAGCTGG CAGATGGGCT GTTGGTGATC CCGCTGCCAG TGGAAGAGCA ATGCCGGGGG 
GTTCTCTCTG AGCCCCTTCC AGACCTTCAG CTTCTCACAG GAGACATCAG GTATGATGAG 
GCCATGGGGT ACCCCATGGT GCAACAGTGG CGTGTCCGGA GCAATCTCTA TCGTGTGAAG 
CTGAGTACTA TCACCCTCTC AGCAGGCTTC ACCAATGTCC TAAAGATCCT GACCAAAGAG 
AGCAGTCGGG ATGAGCTGCT GTCCTTCATC CAGCACTATG GCTCCCACTA CATTGCCGAG 
GCTTTATACG GTTCTGAGCT CACCTGCATC ATCCATTTTC CCAGCAAGAA GGTCCAGCAG 
CAGCTATGGC TCCAATATCA GAAAGGTGTA AAGGCCCAAG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538168.1
CDS	172..3036
Translation	MAAAGARRSPGRGLGLRGRPRLGFHPGPPPPPPPPLLLLFLLLLPPPPLLAGATAAAASREPDSPCRLKTVTVSTLPALRESDIGWSGARTGAAAGAGAGTGAGAGAAAAAASAASPGSAGSAGTAAESRLLLFVRNELPGRIAVQDDLDNTELPFFTLEMSGTAADISLVHWRQQWLENGTLYFHVSMSSSGQLAQATAPTLQEPSEIVEEQMHILHISVMGGLIALLLLLLVFTVALYAQRRWQKRRRIPQKSASTEATHEIHYIPSVLLGPQARESFRSSRLQTHNSVIGVPIRETPILDDYDYEEEEEPPRRANHVSREDEFGSQMTHALDSLGRPGEEKVEFEKKAAAEATQETVESLMQKFKESFRANTPVEIGQLQPASRSSTSAGKRKRRNKSRGGISFGRTKGTSGSEADDETQLTFYTEQYRSRRRSKGLLKSPVNKTALTLIAVSSCILAMVCGNQMSCPLTVKVTLHVPEHFIADGSSFVVSEGSYLDISDWLNPAKLSLYYQINATSPWVRDLCGQRTTDACEQLCDPDTGECSCHEGYAPDPVHRHLCVRSDWGQSEGPWPYTTLERGYDLVTGEQAPEKILRSTFSLGQGLWLPVSKSFVVPPVELSINPLASCKTDVLVTEDPADVREEAMLSTYFETINDLLSSFGPVRDCSRNNGGCTRNFKCVSDRQVDSSGCVCPEELKPMKDGSGCYDHSKGIDCSDGFNGGCEQLCLQQTLPLPYDTTSSTIFMFCGCVEEYKLAPDGKSCLMLSDVCEGPKCLKPDSKFNDTLFGEMLHGYNNRTQHVNQGQVFQMTFRENNFIKDFPQLADGLLVIPLPVEEQCRGVLSEPLPDLQLLTGDIRYDEAMGYPMVQQWRVRSNLYRVKLSTITLSAGFTNVLKILTKESSRDELLSFIQHYGSHYIAEALYGSELTCIIHFPSKKVQQQLWLQYQKGVKAQG

Target ORF information:

RefSeq Version	XM_006538105.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus astrotactin 2 (Astn2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538105.4

ORF Insert Sequence:

ATGGCCGCCG CCGGCGCCCG GCGCAGCCCG GGCCGCGGCT TGGGGCTTCG GGGGCGGCCG 
AGGCTCGGCT TCCACCCGGG GCCGCCGCCG CCACCGCCGC CGCCGCTGCT GTTGCTGTTT 
CTGCTCCTGC TGCCGCCGCC GCCGTTGCTG GCCGGCGCCA CGGCCGCCGC CGCCTCGCGG 
GAGCCCGACA GCCCATGCCG GCTCAAGACC GTCACCGTAT CCACGCTGCC CGCTCTGCGG 
GAAAGCGACA TCGGCTGGAG CGGTGCCCGC ACCGGGGCCG CGGCCGGGGC TGGGGCCGGG 
ACCGGAGCCG GGGCCGGAGC TGCTGCTGCT GCCGCCTCCG CCGCGTCCCC GGGCTCCGCG 
GGTTCAGCCG GCACAGCCGC GGAGTCTCGT CTCCTTCTCT TTGTGCGTAA TGAGCTCCCC 
GGGCGCATCG CGGTACAAGA TGACCTGGAC AACACTGAGC TGCCCTTCTT CACCCTGGAG 
ATGTCTGGCA CAGCAGCGGA CATTTCTTTG GTTCACTGGA GGCAGCAGTG GCTGGAGAAT 
GGCACATTGT ACTTCCACGT TTCCATGAGC AGCTCTGGGC AACTGGCTCA GGCCACTGCT 
CCCACACTCC AGGAGCCCTC GGAGATCGTT GAGGAACAGA TGCATATCCT CCACATTTCT 
GTGATGGGTG GACTCATTGC ACTTCTCCTC CTGCTGTTGG TGTTCACAGT GGCACTGTAT 
GCCCAGCGAC GCTGGCAGAA GCGCCGGCGC ATCCCACAGA AGAGCGCAAG TACAGAAGCT 
ACTCATGAGA TTCACTACAT CCCATCAGTG CTGCTGGGTC CTCAGGCCAG GGAAAGCTTC 
CGATCCTCCA GACTTCAGAC ACACAACTCA GTCATTGGTG TGCCTATCCG GGAAACCCCC 
ATCCTGGATG ACTATGACTA TGAAGAGGAG GAGGAACCAC CCAGGCGAGC CAACCACGTC 
TCCCGTGAGG ATGAGTTTGG TAGCCAGATG ACCCATGCCC TGGACAGCTT GGGAAGGCCA 
GGAGAAGAGA AAGTGGAATT TGAGAAGAAA GCAGCAGCTG AGGCGACACA GGAAACAGTG 
GAGTCCCTGA TGCAGAAGTT CAAGGAGAGT TTCCGTGCTA ACACACCAGT GGAGATCGGT 
CAGTTGCAGC CAGCCTCGCG CAGCAGCACC TCTGCAGGGA AGAGGAAGCG GAGGAACAAA 
TCTCGAGGGG GAATCAGCTT TGGGAGAACC AAGGGGACAT CAGGCTCAGA AGCAGATGAC 
GAAACACAGC TGACCTTCTA CACAGAGCAG TACCGCAGTC GCCGCCGCAG CAAAGGTTTA 
CTGAAAAGCC CTGTGAATAA GACAGCCTTA ACACTGATTG CTGTGAGCTC CTGCATCTTG 
GCCATGGTGT GTGGCAACCA GATGTCCTGT CCACTTACTG TGAAGGTGAC TTTGCATGTG 
CCTGAACACT TCATTGCAGA TGGGAGCAGC TTTGTGGTGA GTGAAGGAAG CTACCTGGAT 
ATCTCCGATT GGCTAAACCC TGCCAAGCTG TCTCTGTATT ACCAGATCAA TGCCACCTCG 
CCATGGGTGA GAGACCTCTG TGGACAGAGG ACGACAGATG CCTGTGAACA GCTCTGTGAC 
CCAGATACTG GGGAGTGCAG TTGTCATGAA GGTTACGCCC CTGACCCAGT CCACAGACAT 
CTATGTGTTC GCAGTGACTG GGGTCAAAGT GAAGGACCTT GGCCTTACAC AACACTGGAG 
AGGGGCTATG ATCTGGTGAC AGGAGAGCAA GCCCCTGAAA AGATTCTCAG GTCTACTTTC 
AGCTTGGGCC AAGGCCTCTG GCTTCCGGTC AGCAAAAGCT TTGTGGTGCC ACCGGTGGAG 
CTGTCCATCA ACCCCCTGGC CAGCTGCAAG ACGGATGTGC TCGTCACAGA AGACCCTGCG 
GATGTCAGGG AAGAAGCAAT GTTGTCCACG TACTTTGAAA CCATCAACGA CCTGCTGTCC 
TCCTTTGGTC CAGTCCGTGA CTGCTCAAGG AACAACGGGG GCTGTACCCG TAACTTCAAG 
TGTGTGTCTG ACCGCCAGGT GGACTCCTCG GGATGTGTGT GTCCTGAGGA ACTGAAGCCC 
ATGAAGGATG GCTCTGGCTG CTATGATCAC TCCAAGGGCA TCGACTGCTC TGATGGCTTT 
AATGGAGGGT GTGAGCAGCT TTGCCTACAA CAAACACTGC CTTTGCCCTA TGATACCACC 
TCCAGCACCA TCTTCATGTT CTGCGGCTGT GTGGAAGAGT ACAAGCTGGC TCCTGATGGG 
AAATCCTGTC TAATGCTCTC AGATGTCTGT GAGGGCCCCA AGTGCCTCAA ACCTGACTCC 
AAATTCAATG ACACCCTCTT TGGAGAGATG CTACATGGTT ACAACAACCG GACACAACAT 
GTAAACCAAG GCCAAGTCTT CCAAATGACA TTCAGGGAGA ATAACTTCAT CAAGGACTTT 
CCACAGCTGG CAGATGGGCT GTTGGTGATC CCGCTGCCAG TGGAAGAGCA ATGCCGGGGG 
GTTCTCTCTG AGCCCCTTCC AGACCTTCAG CTTCTCACAG GAGACATCAG GTATGATGAG 
GCCATGGGGT ACCCCATGGT GCAACAGTGG CGTGTCCGGA GCAATCTCTA TCGTGTGAAG 
CTGAGTACTA TCACCCTCTC AGCAGGCTTC ACCAATGTCC TAAAGATCCT GACCAAAGAG 
AGCAGTCGGG ATGAGCTGCT GTCCTTCATC CAGCACTATG GCTCCCACTA CATTGCCGAG 
GCTTTATACG GTTCTGAGCT CACCTGCATC ATCCATTTTC CCAGCAAGAA GGTCCAGCAG 
CAGCTATGGC TCCAATATCA GAAAGGTGTA AAGGCCCAAG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Astn2 ( XM_030253680.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109540.1 Mus musculus astrotactin 2 (Astn2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu88592
Clone ID Related Accession (Same CDS sequence)	XM_030253680.1
Accession Version	XM_030253680.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	astrotactin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCG CCGGCGCCCG GCGCAGCCCG GGCCGCGGCT TGGGGCTTCG GGGGCGGCCG 
AGGCTCGGCT TCCACCCGGG GCCGCCGCCG CCACCGCCGC CGCCGCTGCT GTTGCTGTTT 
CTGCTCCTGC TGCCGCCGCC GCCGTTGCTG GCCGGCGCCA CGGCCGCCGC CGCCTCGCGG 
GAGCCCGACA GCCCATGCCG GCTCAAGACC GTCACCGTAT CCACGCTGCC CGCTCTGCGG 
GAAAGCGACA TCGGCTGGAG CGGTGCCCGC ACCGGGGCCG CGGCCGGGGC TGGGGCCGGG 
ACCGGAGCCG GGGCCGGAGC TGCTGCTGCT GCCGCCTCCG CCGCGTCCCC GGGCTCCGCG 
GGTTCAGCCG GCACAGCCGC GGAGTCTCGT CTCCTTCTCT TTGTGCGTAA TGAGCTCCCC 
GGGCGCATCG CGGTACAAGA TGACCTGGAC AACACTGAGC TGCCCTTCTT CACCCTGGAG 
ATGTCTGGCA CAGCAGCGGA CATTTCTTTG GTTCACTGGA GGCAGCAGTG GCTGGAGAAT 
GGCACATTGT ACTTCCACGT TTCCATGAGC AGCTCTGGGC AACTGGCTCA GGCCACTGCT 
CCCACACTCC AGGAGCCCTC GGAGATCGTT GAGGAACAGA TGCATATCCT CCACATTTCT 
GTGATGGGTG GACTCATTGC ACTTCTCCTC CTGCTGTTGG TGTTCACAGT GGCACTGTAT 
GCCCAGCGAC GCTGGCAGAA GCGCCGGCGC ATCCCACAGA AGAGCGCAAG TACAGAAGCT 
ACTCATGAGA TTCACTACAT CCCATCAGTG CTGCTGGGTC CTCAGGCCAG GGAAAGCTTC 
CGATCCTCCA GACTTCAGAC ACACAACTCA GTCATTGGTG TGCCTATCCG GGAAACCCCC 
ATCCTGGATG ACTATGACTA TGAAGAGGAG GAGGAACCAC CCAGGCGAGC CAACCACGTC 
TCCCGTGAGG ATGAGTTTGG TAGCCAGATG ACCCATGCCC TGGACAGCTT GGGAAGGCCA 
GGAGAAGAGA AAGTGGAATT TGAGAAGAAA GGGGGAATCA GCTTTGGGAG AACCAAGGGG 
ACATCAGGCT CAGAAGCAGA TGACGAAACA CAGCTGACCT TCTACACAGA GCAGTACCGC 
AGTCGCCGCC GCAGCAAAGG TTTACTGAAA AGCCCTGTGA ATAAGACAGC CTTAACACTG 
ATTGCTGTGA GCTCCTGCAT CTTGGCCATG GTGTGTGGCA ACCAGATGTC CTGTCCACTT 
ACTGTGAAGG TGACTTTGCA TGTGCCTGAA CACTTCATTG CAGATGGGAG CAGCTTTGTG 
GTGAGTGAAG GAAGCTACCT GGATATCTCC GATTGGCTAA ACCCTGCCAA GCTGTCTCTG 
TATTACCAGA TCAATGCCAC CTCGCCATGG GTGAGAGACC TCTGTGGACA GAGGACGACA 
GATGCCTGTG AACAGCTCTG TGACCCAGAT ACTGGGGAGT GCAGTTGTCA TGAAGGTTAC 
GCCCCTGACC CAGTCCACAG ACATCTATGT GTTCGCAGTG ACTGGGGTCA AAGTGAAGGA 
CCTTGGCCTT ACACAACACT GGAGAGGGGC TATGATCTGG TGACAGGAGA GCAAGCCCCT 
GAAAAGATTC TCAGCTTGGG CCAAGGCCTC TGGCTTCCGG TCAGCAAAAG CTTTGTGGTG 
CCACCGGTGG AGCTGTCCAT CAACCCCCTG GCCAGCTGCA AGACGGATGT GCTCGTCACA 
GAAGACCCTG CGGATGTCAG GGAAGAAGCA ATGTTGTCCA CGTACTTTGA AACCATCAAC 
GACCTGCTGT CCTCCTTTGG TCCAGTCCGT GACTGCTCAA GGAACAACGG GGGCTGTACC 
CGTAACTTCA AGTGTGTGTC TGACCGCCAG GTGGACTCCT CGGGATGTGT GTGTCCTGAG 
GAACTGAAGC CCATGAAGGA TGGCTCTGGC TGCTATGATC ACTCCAAGGG CATCGACTGC 
TCTGATGGCT TTAATGGAGG GTGTGAGCAG CTTTGCCTAC AACAAACACT GCCTTTGCCC 
TATGATACCA CCTCCAGCAC CATCTTCATG TTCTGCGGCT GTGTGGAAGA GTACAAGCTG 
GCTCCTGATG GGAAATCCTG TCTAATGCTC TCAGATGTCT GTGAGGGCCC CAAGTGCCTC 
AAACCTGACT CCAAATTCAA TGACACCCTC TTTGGAGAGA TGCTACATGG TTACAACAAC 
CGGACACAAC ATGTAAACCA AGGCCAAGTC TTCCAAATGA CATTCAGGGA GAATAACTTC 
ATCAAGGACT TTCCACAGCT GGCAGATGGG CTGTTGGTGA TCCCGCTGCC AGTGGAAGAG 
CAATGCCGGG GGGTTCTCTC TGAGCCCCTT CCAGACCTTC AGCTTCTCAC AGGAGACATC 
AGGTATGATG AGGCCATGGG GTACCCCATG GTGCAACAGT GGCGTGTCCG GAGCAATCTC 
TATCGTGTGA AGCTGAGTAC TATCACCCTC TCAGCAGGCT TCACCAATGT CCTAAAGATC 
CTGACCAAAG AGAGCAGTCG GGATGAGCTG CTGTCCTTCA TCCAGCACTA TGGCTCCCAC 
TACATTGCCG AGGCTTTATA CGGTTCTGAG CTCACCTGCA TCATCCATTT TCCCAGCAAG 
AAGGTCCAGC AGCAGCTATG GCTCCAATAT CAGAAAGAGA CCACGGAGCT GGGCAGCAAG 
AAGGAGCTCA AGTCCATGCC CTTCATCACC TACCTCTCCG GTCTCCTGAC AGCCCAGATG 
CTATCAGATG ACCAGCTCAT CTCAGGTGTG GAGATTCGAT GTGAGGAGAA GGGCCGCTGT 
CCATCTACCT GTCACCTTTG CCGCCGGCCA GGCAAAGAGC AGCTGAGCCC CACACCAGTG 
CTGCTGGAGA TAAACCGTGT GGTTCCACTT TATACCCTCA TCCAAGACAA TGGCACAAAG 
GAGGCCTTCA AGAATGCACT GATGAGTTCT TACTGGTGTT CTGGTAAAGG AGATGTGATT 
GATGACTGGT GCAGGTGTGA CCTCAGTGCC TTTGATGCCA GTGGGCTTCC AAATTGTAGC 
CCCCTTCCAC AGCCGGTGTT ACGACTTTCT CCAACAGTGG AGCCTTCCAG CACTGTGGTT 
TCCCTGGAGT GGGTAGATGT TCAGCCAGCC ATTGGAACCA AAGTCTCTGA CTATATTCTG 
CAGCACAAGA AAGTGGATGA ATACACAGAC ACGGACCTAT ACACAGGAGA ATTCCTGAGT 
TTTGCCGATG ACTTACTTTC TGGCCTGGGT ACATCTTGTG TAGCAGCTGG TCGAAGCCAT 
GGAGAGGTTC CTGAAGTCAG TATCTACTCG GTGATCTTCA AGTGCCTGGA GCCAGATGGT 
CTTTACAAGT TCACTCTGTA TGCTGTGGAT ACTCGAGGGA GGCACTCAGA GCTCAGCACA 
GTGACTCTAA GGACAGCTTG TCCACTGGTA GATGACAACA AAGCAGAAGA GATAGCGGAC 
AAGATTTACA ATCTATACAA TGGTTACACA AGTGGCAAGG AGCAGCAGAC TGCTTACAAC 
ACACTGATGG AAGTCTCCGC CTCCATGCTC TTCCGTGTCC AGCACCACTA CAACTCTCAC 
TATGAAAAGT TTGGTGACTT CGTCTGGAGG AGTGAGGATG AGCTAGGACC AAGGAAGGCT 
CATCTGATTC TGCGGCGGCT GGAGCGGGTC AGCAGCCACT GTTCTAGTCT CTTGAGAAGC 
GCCTACATCC AGAGTCGTGT GGACACCATA CCCTATCTCT TCTGCCGCAG TGAGGAGGTC 
CGGCCTGCAG GAATGGTGTG GTACAGCATC CTCAAAGACA CCAAAATCAC ATGTGAGGAG 
AAGATGGTGT CTATGGCCCG AAACACGTAC GGGGAAACCA AAGGCCGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109540.1
CDS	178..4068
Translation	MAAAGARRSPGRGLGLRGRPRLGFHPGPPPPPPPPLLLLFLLLLPPPPLLAGATAAAASREPDSPCRLKTVTVSTLPALRESDIGWSGARTGAAAGAGAGTGAGAGAAAAAASAASPGSAGSAGTAAESRLLLFVRNELPGRIAVQDDLDNTELPFFTLEMSGTAADISLVHWRQQWLENGTLYFHVSMSSSGQLAQATAPTLQEPSEIVEEQMHILHISVMGGLIALLLLLLVFTVALYAQRRWQKRRRIPQKSASTEATHEIHYIPSVLLGPQARESFRSSRLQTHNSVIGVPIRETPILDDYDYEEEEEPPRRANHVSREDEFGSQMTHALDSLGRPGEEKVEFEKKGGISFGRTKGTSGSEADDETQLTFYTEQYRSRRRSKGLLKSPVNKTALTLIAVSSCILAMVCGNQMSCPLTVKVTLHVPEHFIADGSSFVVSEGSYLDISDWLNPAKLSLYYQINATSPWVRDLCGQRTTDACEQLCDPDTGECSCHEGYAPDPVHRHLCVRSDWGQSEGPWPYTTLERGYDLVTGEQAPEKILSLGQGLWLPVSKSFVVPPVELSINPLASCKTDVLVTEDPADVREEAMLSTYFETINDLLSSFGPVRDCSRNNGGCTRNFKCVSDRQVDSSGCVCPEELKPMKDGSGCYDHSKGIDCSDGFNGGCEQLCLQQTLPLPYDTTSSTIFMFCGCVEEYKLAPDGKSCLMLSDVCEGPKCLKPDSKFNDTLFGEMLHGYNNRTQHVNQGQVFQMTFRENNFIKDFPQLADGLLVIPLPVEEQCRGVLSEPLPDLQLLTGDIRYDEAMGYPMVQQWRVRSNLYRVKLSTITLSAGFTNVLKILTKESSRDELLSFIQHYGSHYIAEALYGSELTCIIHFPSKKVQQQLWLQYQKETTELGSKKELKSMPFITYLSGLLTAQMLSDDQLISGVEIRCEEKGRCPSTCHLCRRPGKEQLSPTPVLLEINRVVPLYTLIQDNGTKEAFKNALMSSYWCSGKGDVIDDWCRCDLSAFDASGLPNCSPLPQPVLRLSPTVEPSSTVVSLEWVDVQPAIGTKVSDYILQHKKVDEYTDTDLYTGEFLSFADDLLSGLGTSCVAAGRSHGEVPEVSIYSVIFKCLEPDGLYKFTLYAVDTRGRHSELSTVTLRTACPLVDDNKAEEIADKIYNLYNGYTSGKEQQTAYNTLMEVSASMLFRVQHHYNSHYEKFGDFVWRSEDELGPRKAHLILRRLERVSSHCSSLLRSAYIQSRVDTIPYLFCRSEEVRPAGMVWYSILKDTKITCEEKMVSMARNTYGETKGR

Target ORF information:

RefSeq Version	XM_030253680.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus astrotactin 2 (Astn2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253680.1

ORF Insert Sequence:

ATGGCCGCCG CCGGCGCCCG GCGCAGCCCG GGCCGCGGCT TGGGGCTTCG GGGGCGGCCG 
AGGCTCGGCT TCCACCCGGG GCCGCCGCCG CCACCGCCGC CGCCGCTGCT GTTGCTGTTT 
CTGCTCCTGC TGCCGCCGCC GCCGTTGCTG GCCGGCGCCA CGGCCGCCGC CGCCTCGCGG 
GAGCCCGACA GCCCATGCCG GCTCAAGACC GTCACCGTAT CCACGCTGCC CGCTCTGCGG 
GAAAGCGACA TCGGCTGGAG CGGTGCCCGC ACCGGGGCCG CGGCCGGGGC TGGGGCCGGG 
ACCGGAGCCG GGGCCGGAGC TGCTGCTGCT GCCGCCTCCG CCGCGTCCCC GGGCTCCGCG 
GGTTCAGCCG GCACAGCCGC GGAGTCTCGT CTCCTTCTCT TTGTGCGTAA TGAGCTCCCC 
GGGCGCATCG CGGTACAAGA TGACCTGGAC AACACTGAGC TGCCCTTCTT CACCCTGGAG 
ATGTCTGGCA CAGCAGCGGA CATTTCTTTG GTTCACTGGA GGCAGCAGTG GCTGGAGAAT 
GGCACATTGT ACTTCCACGT TTCCATGAGC AGCTCTGGGC AACTGGCTCA GGCCACTGCT 
CCCACACTCC AGGAGCCCTC GGAGATCGTT GAGGAACAGA TGCATATCCT CCACATTTCT 
GTGATGGGTG GACTCATTGC ACTTCTCCTC CTGCTGTTGG TGTTCACAGT GGCACTGTAT 
GCCCAGCGAC GCTGGCAGAA GCGCCGGCGC ATCCCACAGA AGAGCGCAAG TACAGAAGCT 
ACTCATGAGA TTCACTACAT CCCATCAGTG CTGCTGGGTC CTCAGGCCAG GGAAAGCTTC 
CGATCCTCCA GACTTCAGAC ACACAACTCA GTCATTGGTG TGCCTATCCG GGAAACCCCC 
ATCCTGGATG ACTATGACTA TGAAGAGGAG GAGGAACCAC CCAGGCGAGC CAACCACGTC 
TCCCGTGAGG ATGAGTTTGG TAGCCAGATG ACCCATGCCC TGGACAGCTT GGGAAGGCCA 
GGAGAAGAGA AAGTGGAATT TGAGAAGAAA GGGGGAATCA GCTTTGGGAG AACCAAGGGG 
ACATCAGGCT CAGAAGCAGA TGACGAAACA CAGCTGACCT TCTACACAGA GCAGTACCGC 
AGTCGCCGCC GCAGCAAAGG TTTACTGAAA AGCCCTGTGA ATAAGACAGC CTTAACACTG 
ATTGCTGTGA GCTCCTGCAT CTTGGCCATG GTGTGTGGCA ACCAGATGTC CTGTCCACTT 
ACTGTGAAGG TGACTTTGCA TGTGCCTGAA CACTTCATTG CAGATGGGAG CAGCTTTGTG 
GTGAGTGAAG GAAGCTACCT GGATATCTCC GATTGGCTAA ACCCTGCCAA GCTGTCTCTG 
TATTACCAGA TCAATGCCAC CTCGCCATGG GTGAGAGACC TCTGTGGACA GAGGACGACA 
GATGCCTGTG AACAGCTCTG TGACCCAGAT ACTGGGGAGT GCAGTTGTCA TGAAGGTTAC 
GCCCCTGACC CAGTCCACAG ACATCTATGT GTTCGCAGTG ACTGGGGTCA AAGTGAAGGA 
CCTTGGCCTT ACACAACACT GGAGAGGGGC TATGATCTGG TGACAGGAGA GCAAGCCCCT 
GAAAAGATTC TCAGCTTGGG CCAAGGCCTC TGGCTTCCGG TCAGCAAAAG CTTTGTGGTG 
CCACCGGTGG AGCTGTCCAT CAACCCCCTG GCCAGCTGCA AGACGGATGT GCTCGTCACA 
GAAGACCCTG CGGATGTCAG GGAAGAAGCA ATGTTGTCCA CGTACTTTGA AACCATCAAC 
GACCTGCTGT CCTCCTTTGG TCCAGTCCGT GACTGCTCAA GGAACAACGG GGGCTGTACC 
CGTAACTTCA AGTGTGTGTC TGACCGCCAG GTGGACTCCT CGGGATGTGT GTGTCCTGAG 
GAACTGAAGC CCATGAAGGA TGGCTCTGGC TGCTATGATC ACTCCAAGGG CATCGACTGC 
TCTGATGGCT TTAATGGAGG GTGTGAGCAG CTTTGCCTAC AACAAACACT GCCTTTGCCC 
TATGATACCA CCTCCAGCAC CATCTTCATG TTCTGCGGCT GTGTGGAAGA GTACAAGCTG 
GCTCCTGATG GGAAATCCTG TCTAATGCTC TCAGATGTCT GTGAGGGCCC CAAGTGCCTC 
AAACCTGACT CCAAATTCAA TGACACCCTC TTTGGAGAGA TGCTACATGG TTACAACAAC 
CGGACACAAC ATGTAAACCA AGGCCAAGTC TTCCAAATGA CATTCAGGGA GAATAACTTC 
ATCAAGGACT TTCCACAGCT GGCAGATGGG CTGTTGGTGA TCCCGCTGCC AGTGGAAGAG 
CAATGCCGGG GGGTTCTCTC TGAGCCCCTT CCAGACCTTC AGCTTCTCAC AGGAGACATC 
AGGTATGATG AGGCCATGGG GTACCCCATG GTGCAACAGT GGCGTGTCCG GAGCAATCTC 
TATCGTGTGA AGCTGAGTAC TATCACCCTC TCAGCAGGCT TCACCAATGT CCTAAAGATC 
CTGACCAAAG AGAGCAGTCG GGATGAGCTG CTGTCCTTCA TCCAGCACTA TGGCTCCCAC 
TACATTGCCG AGGCTTTATA CGGTTCTGAG CTCACCTGCA TCATCCATTT TCCCAGCAAG 
AAGGTCCAGC AGCAGCTATG GCTCCAATAT CAGAAAGAGA CCACGGAGCT GGGCAGCAAG 
AAGGAGCTCA AGTCCATGCC CTTCATCACC TACCTCTCCG GTCTCCTGAC AGCCCAGATG 
CTATCAGATG ACCAGCTCAT CTCAGGTGTG GAGATTCGAT GTGAGGAGAA GGGCCGCTGT 
CCATCTACCT GTCACCTTTG CCGCCGGCCA GGCAAAGAGC AGCTGAGCCC CACACCAGTG 
CTGCTGGAGA TAAACCGTGT GGTTCCACTT TATACCCTCA TCCAAGACAA TGGCACAAAG 
GAGGCCTTCA AGAATGCACT GATGAGTTCT TACTGGTGTT CTGGTAAAGG AGATGTGATT 
GATGACTGGT GCAGGTGTGA CCTCAGTGCC TTTGATGCCA GTGGGCTTCC AAATTGTAGC 
CCCCTTCCAC AGCCGGTGTT ACGACTTTCT CCAACAGTGG AGCCTTCCAG CACTGTGGTT 
TCCCTGGAGT GGGTAGATGT TCAGCCAGCC ATTGGAACCA AAGTCTCTGA CTATATTCTG 
CAGCACAAGA AAGTGGATGA ATACACAGAC ACGGACCTAT ACACAGGAGA ATTCCTGAGT 
TTTGCCGATG ACTTACTTTC TGGCCTGGGT ACATCTTGTG TAGCAGCTGG TCGAAGCCAT 
GGAGAGGTTC CTGAAGTCAG TATCTACTCG GTGATCTTCA AGTGCCTGGA GCCAGATGGT 
CTTTACAAGT TCACTCTGTA TGCTGTGGAT ACTCGAGGGA GGCACTCAGA GCTCAGCACA 
GTGACTCTAA GGACAGCTTG TCCACTGGTA GATGACAACA AAGCAGAAGA GATAGCGGAC 
AAGATTTACA ATCTATACAA TGGTTACACA AGTGGCAAGG AGCAGCAGAC TGCTTACAAC 
ACACTGATGG AAGTCTCCGC CTCCATGCTC TTCCGTGTCC AGCACCACTA CAACTCTCAC 
TATGAAAAGT TTGGTGACTT CGTCTGGAGG AGTGAGGATG AGCTAGGACC AAGGAAGGCT 
CATCTGATTC TGCGGCGGCT GGAGCGGGTC AGCAGCCACT GTTCTAGTCT CTTGAGAAGC 
GCCTACATCC AGAGTCGTGT GGACACCATA CCCTATCTCT TCTGCCGCAG TGAGGAGGTC 
CGGCCTGCAG GAATGGTGTG GTACAGCATC CTCAAAGACA CCAAAATCAC ATGTGAGGAG 
AAGATGGTGT CTATGGCCCG AAACACGTAC GGGGAAACCA AAGGCCGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Astn2 ( NM_019514.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062387.2 Mus musculus astrotactin 2 (Astn2), transcript variant 1, mRNA.

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CloneID	OMu13426
Clone ID Related Accession (Same CDS sequence)	NM_019514.3
Accession Version	NM_019514.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	astrotactin-2 isoform a precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL713878.15, AL731829.10, AL691475.18, AL691438.16, AL691474.29, AL691456.7 and AL691454.15. On Nov 17, 2006 this sequence version replaced NM_019514.2. Transcript Variant: This variant (1) lacks an alternate segment in the 5' coding region, compared to variant 2. The resulting protein (isoform a) has a shorter N-terminus when compared to isoform b. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGCCG CCGGCGCCCG GCGCAGCCCG GGCCGCGGCT TGGGGCTTCG GGGGCGGCCG 
AGGCTCGGCT TCCACCCGGG GCCGCCGCCG CCACCGCCGC CGCCGCTGCT GTTGCTGTTT 
CTGCTCCTGC TGCCGCCGCC GCCGTTGCTG GCCGGCGCCA CGGCCGCCGC CGCCTCGCGG 
GAGCCCGACA GCCCATGCCG GCTCAAGACC GTCACCGTAT CCACGCTGCC CGCTCTGCGG 
GAAAGCGACA TCGGCTGGAG CGGTGCCCGC ACCGGGGCCG CGGCCGGGGC TGGGGCCGGG 
ACCGGAGCCG GGGCCGGAGC TGCTGCTGCT GCCGCCTCCG CCGCGTCCCC GGGCTCCGCG 
GGTTCAGCCG GCACAGCCGC GGAGTCTCGT CTCCTTCTCT TTGTGCGTAA TGAGCTCCCC 
GGGCGCATCG CGGTACAAGA TGACCTGGAC AACACTGAGC TGCCCTTCTT CACCCTGGAG 
ATGTCTGGCA CAGCAGCGGA CATTTCTTTG GTTCACTGGA GGCAGCAGTG GCTGGAGAAT 
GGCACATTGT ACTTCCACGT TTCCATGAGC AGCTCTGGGC AACTGGCTCA GGCCACTGCT 
CCCACACTCC AGGAGCCCTC GGAGATCGTT GAGGAACAGA TGCATATCCT CCACATTTCT 
GTGATGGGTG GACTCATTGC ACTTCTCCTC CTGCTGTTGG TGTTCACAGT GGCACTGTAT 
GCCCAGCGAC GCTGGCAGAA GCGCCGGCGC ATCCCACAGA AGAGCGCAAG TACAGAAGCT 
ACTCATGAGA TTCACTACAT CCCATCAGTG CTGCTGGGTC CTCAGGCCAG GGAAAGCTTC 
CGATCCTCCA GACTTCAGAC ACACAACTCA GTCATTGGTG TGCCTATCCG GGAAACCCCC 
ATCCTGGATG ACTATGACTA TGAAGAGGAG GAGGAACCAC CCAGGCGAGC CAACCACGTC 
TCCCGTGAGG ATGAGTTTGG TAGCCAGATG ACCCATGCCC TGGACAGCTT GGGAAGGCCA 
GGAGAAGAGA AAGTGGAATT TGAGAAGAAA GGGGGAATCA GCTTTGGGAG AACCAAGGGG 
ACATCAGGCT CAGAAGCAGA TGACGAAACA CAGCTGACCT TCTACACAGA GCAGTACCGC 
AGTCGCCGCC GCAGCAAAGG TTTACTGAAA AGCCCTGTGA ATAAGACAGC CTTAACACTG 
ATTGCTGTGA GCTCCTGCAT CTTGGCCATG GTGTGTGGCA ACCAGATGTC CTGTCCACTT 
ACTGTGAAGG TGACTTTGCA TGTGCCTGAA CACTTCATTG CAGATGGGAG CAGCTTTGTG 
GTGAGTGAAG GAAGCTACCT GGATATCTCC GATTGGCTAA ACCCTGCCAA GCTGTCTCTG 
TATTACCAGA TCAATGCCAC CTCGCCATGG GTGAGAGACC TCTGTGGACA GAGGACGACA 
GATGCCTGTG AACAGCTCTG TGACCCAGAT ACTGGGGAGT GCAGTTGTCA TGAAGGTTAC 
GCCCCTGACC CAGTCCACAG ACATCTATGT GTTCGCAGTG ACTGGGGTCA AAGTGAAGGA 
CCTTGGCCTT ACACAACACT GGAGAGGGGC TATGATCTGG TGACAGGAGA GCAAGCCCCT 
GAAAAGATTC TCAGGTCTAC TTTCAGCTTG GGCCAAGGCC TCTGGCTTCC GGTCAGCAAA 
AGCTTTGTGG TGCCACCGGT GGAGCTGTCC ATCAACCCCC TGGCCAGCTG CAAGACGGAT 
GTGCTCGTCA CAGAAGACCC TGCGGATGTC AGGGAAGAAG CAATGTTGTC CACGTACTTT 
GAAACCATCA ACGACCTGCT GTCCTCCTTT GGTCCAGTCC GTGACTGCTC AAGGAACAAC 
GGGGGCTGTA CCCGTAACTT CAAGTGTGTG TCTGACCGCC AGGTGGACTC CTCGGGATGT 
GTGTGTCCTG AGGAACTGAA GCCCATGAAG GATGGCTCTG GCTGCTATGA TCACTCCAAG 
GGCATCGACT GCTCTGATGG CTTTAATGGA GGGTGTGAGC AGCTTTGCCT ACAACAAACA 
CTGCCTTTGC CCTATGATAC CACCTCCAGC ACCATCTTCA TGTTCTGCGG CTGTGTGGAA 
GAGTACAAGC TGGCTCCTGA TGGGAAATCC TGTCTAATGC TCTCAGATGT CTGTGAGGGC 
CCCAAGTGCC TCAAACCTGA CTCCAAATTC AATGACACCC TCTTTGGAGA GATGCTACAT 
GGTTACAACA ACCGGACACA ACATGTAAAC CAAGGCCAAG TCTTCCAAAT GACATTCAGG 
GAGAATAACT TCATCAAGGA CTTTCCACAG CTGGCAGATG GGCTGTTGGT GATCCCGCTG 
CCAGTGGAAG AGCAATGCCG GGGGGTTCTC TCTGAGCCCC TTCCAGACCT TCAGCTTCTC 
ACAGGAGACA TCAGGTATGA TGAGGCCATG GGGTACCCCA TGGTGCAACA GTGGCGTGTC 
CGGAGCAATC TCTATCGTGT GAAGCTGAGT ACTATCACCC TCTCAGCAGG CTTCACCAAT 
GTCCTAAAGA TCCTGACCAA AGAGAGCAGT CGGGATGAGC TGCTGTCCTT CATCCAGCAC 
TATGGCTCCC ACTACATTGC CGAGGCTTTA TACGGTTCTG AGCTCACCTG CATCATCCAT 
TTTCCCAGCA AGAAGGTCCA GCAGCAGCTA TGGCTCCAAT ATCAGAAAGA GACCACGGAG 
CTGGGCAGCA AGAAGGAGCT CAAGTCCATG CCCTTCATCA CCTACCTCTC CGGTCTCCTG 
ACAGCCCAGA TGCTATCAGA TGACCAGCTC ATCTCAGGTG TGGAGATTCG ATGTGAGGAG 
AAGGGCCGCT GTCCATCTAC CTGTCACCTT TGCCGCCGGC CAGGCAAAGA GCAGCTGAGC 
CCCACACCAG TGCTGCTGGA GATAAACCGT GTGGTTCCAC TTTATACCCT CATCCAAGAC 
AATGGCACAA AGGAGGCCTT CAAGAATGCA CTGATGAGTT CTTACTGGTG TTCTGGTAAA 
GGAGATGTGA TTGATGACTG GTGCAGGTGT GACCTCAGTG CCTTTGATGC CAGTGGGCTT 
CCAAATTGTA GCCCCCTTCC ACAGCCGGTG TTACGACTTT CTCCAACAGT GGAGCCTTCC 
AGCACTGTGG TTTCCCTGGA GTGGGTAGAT GTTCAGCCAG CCATTGGAAC CAAAGTCTCT 
GACTATATTC TGCAGCACAA GAAAGTGGAT GAATACACAG ACACGGACCT ATACACAGGA 
GAATTCCTGA GTTTTGCCGA TGACTTACTT TCTGGCCTGG GTACATCTTG TGTAGCAGCT 
GGTCGAAGCC ATGGAGAGGT TCCTGAAGTC AGTATCTACT CGGTGATCTT CAAGTGCCTG 
GAGCCAGATG GTCTTTACAA GTTCACTCTG TATGCTGTGG ATACTCGAGG GAGGCACTCA 
GAGCTCAGCA CAGTGACTCT AAGGACAGCT TGTCCACTGG TAGATGACAA CAAAGCAGAA 
GAGATAGCGG ACAAGATTTA CAATCTATAC AATGGTTACA CAAGTGGCAA GGAGCAGCAG 
ACTGCTTACA ACACACTGAT GGAAGTCTCC GCCTCCATGC TCTTCCGTGT CCAGCACCAC 
TACAACTCTC ACTATGAAAA GTTTGGTGAC TTCGTCTGGA GGAGTGAGGA TGAGCTAGGA 
CCAAGGAAGG CTCATCTGAT TCTGCGGCGG CTGGAGCGGG TCAGCAGCCA CTGTTCTAGT 
CTCTTGAGAA GCGCCTACAT CCAGAGTCGT GTGGACACCA TACCCTATCT CTTCTGCCGC 
AGTGAGGAGG TCCGGCCTGC AGGAATGGTG TGGTACAGCA TCCTCAAAGA CACCAAAATC 
ACATGTGAGG AGAAGATGGT GTCTATGGCC CGAAACACGT ACGGGGAAAC CAAAGGCCGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062387.2
CDS	124..4026
Translation	MAAAGARRSPGRGLGLRGRPRLGFHPGPPPPPPPPLLLLFLLLLPPPPLLAGATAAAASREPDSPCRLKTVTVSTLPALRESDIGWSGARTGAAAGAGAGTGAGAGAAAAAASAASPGSAGSAGTAAESRLLLFVRNELPGRIAVQDDLDNTELPFFTLEMSGTAADISLVHWRQQWLENGTLYFHVSMSSSGQLAQATAPTLQEPSEIVEEQMHILHISVMGGLIALLLLLLVFTVALYAQRRWQKRRRIPQKSASTEATHEIHYIPSVLLGPQARESFRSSRLQTHNSVIGVPIRETPILDDYDYEEEEEPPRRANHVSREDEFGSQMTHALDSLGRPGEEKVEFEKKGGISFGRTKGTSGSEADDETQLTFYTEQYRSRRRSKGLLKSPVNKTALTLIAVSSCILAMVCGNQMSCPLTVKVTLHVPEHFIADGSSFVVSEGSYLDISDWLNPAKLSLYYQINATSPWVRDLCGQRTTDACEQLCDPDTGECSCHEGYAPDPVHRHLCVRSDWGQSEGPWPYTTLERGYDLVTGEQAPEKILRSTFSLGQGLWLPVSKSFVVPPVELSINPLASCKTDVLVTEDPADVREEAMLSTYFETINDLLSSFGPVRDCSRNNGGCTRNFKCVSDRQVDSSGCVCPEELKPMKDGSGCYDHSKGIDCSDGFNGGCEQLCLQQTLPLPYDTTSSTIFMFCGCVEEYKLAPDGKSCLMLSDVCEGPKCLKPDSKFNDTLFGEMLHGYNNRTQHVNQGQVFQMTFRENNFIKDFPQLADGLLVIPLPVEEQCRGVLSEPLPDLQLLTGDIRYDEAMGYPMVQQWRVRSNLYRVKLSTITLSAGFTNVLKILTKESSRDELLSFIQHYGSHYIAEALYGSELTCIIHFPSKKVQQQLWLQYQKETTELGSKKELKSMPFITYLSGLLTAQMLSDDQLISGVEIRCEEKGRCPSTCHLCRRPGKEQLSPTPVLLEINRVVPLYTLIQDNGTKEAFKNALMSSYWCSGKGDVIDDWCRCDLSAFDASGLPNCSPLPQPVLRLSPTVEPSSTVVSLEWVDVQPAIGTKVSDYILQHKKVDEYTDTDLYTGEFLSFADDLLSGLGTSCVAAGRSHGEVPEVSIYSVIFKCLEPDGLYKFTLYAVDTRGRHSELSTVTLRTACPLVDDNKAEEIADKIYNLYNGYTSGKEQQTAYNTLMEVSASMLFRVQHHYNSHYEKFGDFVWRSEDELGPRKAHLILRRLERVSSHCSSLLRSAYIQSRVDTIPYLFCRSEEVRPAGMVWYSILKDTKITCEEKMVSMARNTYGETKGR

Target ORF information:

RefSeq Version	NM_019514.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus astrotactin 2 (Astn2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019514.3

ORF Insert Sequence:

ATGGCCGCCG CCGGCGCCCG GCGCAGCCCG GGCCGCGGCT TGGGGCTTCG GGGGCGGCCG 
AGGCTCGGCT TCCACCCGGG GCCGCCGCCG CCACCGCCGC CGCCGCTGCT GTTGCTGTTT 
CTGCTCCTGC TGCCGCCGCC GCCGTTGCTG GCCGGCGCCA CGGCCGCCGC CGCCTCGCGG 
GAGCCCGACA GCCCATGCCG GCTCAAGACC GTCACCGTAT CCACGCTGCC CGCTCTGCGG 
GAAAGCGACA TCGGCTGGAG CGGTGCCCGC ACCGGGGCCG CGGCCGGGGC TGGGGCCGGG 
ACCGGAGCCG GGGCCGGAGC TGCTGCTGCT GCCGCCTCCG CCGCGTCCCC GGGCTCCGCG 
GGTTCAGCCG GCACAGCCGC GGAGTCTCGT CTCCTTCTCT TTGTGCGTAA TGAGCTCCCC 
GGGCGCATCG CGGTACAAGA TGACCTGGAC AACACTGAGC TGCCCTTCTT CACCCTGGAG 
ATGTCTGGCA CAGCAGCGGA CATTTCTTTG GTTCACTGGA GGCAGCAGTG GCTGGAGAAT 
GGCACATTGT ACTTCCACGT TTCCATGAGC AGCTCTGGGC AACTGGCTCA GGCCACTGCT 
CCCACACTCC AGGAGCCCTC GGAGATCGTT GAGGAACAGA TGCATATCCT CCACATTTCT 
GTGATGGGTG GACTCATTGC ACTTCTCCTC CTGCTGTTGG TGTTCACAGT GGCACTGTAT 
GCCCAGCGAC GCTGGCAGAA GCGCCGGCGC ATCCCACAGA AGAGCGCAAG TACAGAAGCT 
ACTCATGAGA TTCACTACAT CCCATCAGTG CTGCTGGGTC CTCAGGCCAG GGAAAGCTTC 
CGATCCTCCA GACTTCAGAC ACACAACTCA GTCATTGGTG TGCCTATCCG GGAAACCCCC 
ATCCTGGATG ACTATGACTA TGAAGAGGAG GAGGAACCAC CCAGGCGAGC CAACCACGTC 
TCCCGTGAGG ATGAGTTTGG TAGCCAGATG ACCCATGCCC TGGACAGCTT GGGAAGGCCA 
GGAGAAGAGA AAGTGGAATT TGAGAAGAAA GGGGGAATCA GCTTTGGGAG AACCAAGGGG 
ACATCAGGCT CAGAAGCAGA TGACGAAACA CAGCTGACCT TCTACACAGA GCAGTACCGC 
AGTCGCCGCC GCAGCAAAGG TTTACTGAAA AGCCCTGTGA ATAAGACAGC CTTAACACTG 
ATTGCTGTGA GCTCCTGCAT CTTGGCCATG GTGTGTGGCA ACCAGATGTC CTGTCCACTT 
ACTGTGAAGG TGACTTTGCA TGTGCCTGAA CACTTCATTG CAGATGGGAG CAGCTTTGTG 
GTGAGTGAAG GAAGCTACCT GGATATCTCC GATTGGCTAA ACCCTGCCAA GCTGTCTCTG 
TATTACCAGA TCAATGCCAC CTCGCCATGG GTGAGAGACC TCTGTGGACA GAGGACGACA 
GATGCCTGTG AACAGCTCTG TGACCCAGAT ACTGGGGAGT GCAGTTGTCA TGAAGGTTAC 
GCCCCTGACC CAGTCCACAG ACATCTATGT GTTCGCAGTG ACTGGGGTCA AAGTGAAGGA 
CCTTGGCCTT ACACAACACT GGAGAGGGGC TATGATCTGG TGACAGGAGA GCAAGCCCCT 
GAAAAGATTC TCAGGTCTAC TTTCAGCTTG GGCCAAGGCC TCTGGCTTCC GGTCAGCAAA 
AGCTTTGTGG TGCCACCGGT GGAGCTGTCC ATCAACCCCC TGGCCAGCTG CAAGACGGAT 
GTGCTCGTCA CAGAAGACCC TGCGGATGTC AGGGAAGAAG CAATGTTGTC CACGTACTTT 
GAAACCATCA ACGACCTGCT GTCCTCCTTT GGTCCAGTCC GTGACTGCTC AAGGAACAAC 
GGGGGCTGTA CCCGTAACTT CAAGTGTGTG TCTGACCGCC AGGTGGACTC CTCGGGATGT 
GTGTGTCCTG AGGAACTGAA GCCCATGAAG GATGGCTCTG GCTGCTATGA TCACTCCAAG 
GGCATCGACT GCTCTGATGG CTTTAATGGA GGGTGTGAGC AGCTTTGCCT ACAACAAACA 
CTGCCTTTGC CCTATGATAC CACCTCCAGC ACCATCTTCA TGTTCTGCGG CTGTGTGGAA 
GAGTACAAGC TGGCTCCTGA TGGGAAATCC TGTCTAATGC TCTCAGATGT CTGTGAGGGC 
CCCAAGTGCC TCAAACCTGA CTCCAAATTC AATGACACCC TCTTTGGAGA GATGCTACAT 
GGTTACAACA ACCGGACACA ACATGTAAAC CAAGGCCAAG TCTTCCAAAT GACATTCAGG 
GAGAATAACT TCATCAAGGA CTTTCCACAG CTGGCAGATG GGCTGTTGGT GATCCCGCTG 
CCAGTGGAAG AGCAATGCCG GGGGGTTCTC TCTGAGCCCC TTCCAGACCT TCAGCTTCTC 
ACAGGAGACA TCAGGTATGA TGAGGCCATG GGGTACCCCA TGGTGCAACA GTGGCGTGTC 
CGGAGCAATC TCTATCGTGT GAAGCTGAGT ACTATCACCC TCTCAGCAGG CTTCACCAAT 
GTCCTAAAGA TCCTGACCAA AGAGAGCAGT CGGGATGAGC TGCTGTCCTT CATCCAGCAC 
TATGGCTCCC ACTACATTGC CGAGGCTTTA TACGGTTCTG AGCTCACCTG CATCATCCAT 
TTTCCCAGCA AGAAGGTCCA GCAGCAGCTA TGGCTCCAAT ATCAGAAAGA GACCACGGAG 
CTGGGCAGCA AGAAGGAGCT CAAGTCCATG CCCTTCATCA CCTACCTCTC CGGTCTCCTG 
ACAGCCCAGA TGCTATCAGA TGACCAGCTC ATCTCAGGTG TGGAGATTCG ATGTGAGGAG 
AAGGGCCGCT GTCCATCTAC CTGTCACCTT TGCCGCCGGC CAGGCAAAGA GCAGCTGAGC 
CCCACACCAG TGCTGCTGGA GATAAACCGT GTGGTTCCAC TTTATACCCT CATCCAAGAC 
AATGGCACAA AGGAGGCCTT CAAGAATGCA CTGATGAGTT CTTACTGGTG TTCTGGTAAA 
GGAGATGTGA TTGATGACTG GTGCAGGTGT GACCTCAGTG CCTTTGATGC CAGTGGGCTT 
CCAAATTGTA GCCCCCTTCC ACAGCCGGTG TTACGACTTT CTCCAACAGT GGAGCCTTCC 
AGCACTGTGG TTTCCCTGGA GTGGGTAGAT GTTCAGCCAG CCATTGGAAC CAAAGTCTCT 
GACTATATTC TGCAGCACAA GAAAGTGGAT GAATACACAG ACACGGACCT ATACACAGGA 
GAATTCCTGA GTTTTGCCGA TGACTTACTT TCTGGCCTGG GTACATCTTG TGTAGCAGCT 
GGTCGAAGCC ATGGAGAGGT TCCTGAAGTC AGTATCTACT CGGTGATCTT CAAGTGCCTG 
GAGCCAGATG GTCTTTACAA GTTCACTCTG TATGCTGTGG ATACTCGAGG GAGGCACTCA 
GAGCTCAGCA CAGTGACTCT AAGGACAGCT TGTCCACTGG TAGATGACAA CAAAGCAGAA 
GAGATAGCGG ACAAGATTTA CAATCTATAC AATGGTTACA CAAGTGGCAA GGAGCAGCAG 
ACTGCTTACA ACACACTGAT GGAAGTCTCC GCCTCCATGC TCTTCCGTGT CCAGCACCAC 
TACAACTCTC ACTATGAAAA GTTTGGTGAC TTCGTCTGGA GGAGTGAGGA TGAGCTAGGA 
CCAAGGAAGG CTCATCTGAT TCTGCGGCGG CTGGAGCGGG TCAGCAGCCA CTGTTCTAGT 
CTCTTGAGAA GCGCCTACAT CCAGAGTCGT GTGGACACCA TACCCTATCT CTTCTGCCGC 
AGTGAGGAGG TCCGGCCTGC AGGAATGGTG TGGTACAGCA TCCTCAAAGA CACCAAAATC 
ACATGTGAGG AGAAGATGGT GTCTATGGCC CGAAACACGT ACGGGGAAAC CAAAGGCCGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Astn2 ( NM_207109.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_996992.1 Mus musculus astrotactin 2 (Astn2), transcript variant 2, mRNA.

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CloneID	OMu13528
Clone ID Related Accession (Same CDS sequence)	NM_207109.2
Accession Version	NM_207109.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	astrotactin-2 isoform b precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL713878.15, AL731829.10, AL691475.18, AL691438.16, AL691474.29, AL691456.7 and AL691454.15. On Nov 17, 2006 this sequence version replaced NM_207109.1. Transcript Variant: This variant (2) represents the longer transcript and it encodes the longer isoform (b). Isoform b is most similar to the protein encoded by transcript variant 3 of the human gene. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGCCG CCGGCGCCCG GCGCAGCCCG GGCCGCGGCT TGGGGCTTCG GGGGCGGCCG 
AGGCTCGGCT TCCACCCGGG GCCGCCGCCG CCACCGCCGC CGCCGCTGCT GTTGCTGTTT 
CTGCTCCTGC TGCCGCCGCC GCCGTTGCTG GCCGGCGCCA CGGCCGCCGC CGCCTCGCGG 
GAGCCCGACA GCCCATGCCG GCTCAAGACC GTCACCGTAT CCACGCTGCC CGCTCTGCGG 
GAAAGCGACA TCGGCTGGAG CGGTGCCCGC ACCGGGGCCG CGGCCGGGGC TGGGGCCGGG 
ACCGGAGCCG GGGCCGGAGC TGCTGCTGCT GCCGCCTCCG CCGCGTCCCC GGGCTCCGCG 
GGTTCAGCCG GCACAGCCGC GGAGTCTCGT CTCCTTCTCT TTGTGCGTAA TGAGCTCCCC 
GGGCGCATCG CGGTACAAGA TGACCTGGAC AACACTGAGC TGCCCTTCTT CACCCTGGAG 
ATGTCTGGCA CAGCAGCGGA CATTTCTTTG GTTCACTGGA GGCAGCAGTG GCTGGAGAAT 
GGCACATTGT ACTTCCACGT TTCCATGAGC AGCTCTGGGC AACTGGCTCA GGCCACTGCT 
CCCACACTCC AGGAGCCCTC GGAGATCGTT GAGGAACAGA TGCATATCCT CCACATTTCT 
GTGATGGGTG GACTCATTGC ACTTCTCCTC CTGCTGTTGG TGTTCACAGT GGCACTGTAT 
GCCCAGCGAC GCTGGCAGAA GCGCCGGCGC ATCCCACAGA AGAGCGCAAG TACAGAAGCT 
ACTCATGAGA TTCACTACAT CCCATCAGTG CTGCTGGGTC CTCAGGCCAG GGAAAGCTTC 
CGATCCTCCA GACTTCAGAC ACACAACTCA GTCATTGGTG TGCCTATCCG GGAAACCCCC 
ATCCTGGATG ACTATGACTA TGAAGAGGAG GAGGAACCAC CCAGGCGAGC CAACCACGTC 
TCCCGTGAGG ATGAGTTTGG TAGCCAGATG ACCCATGCCC TGGACAGCTT GGGAAGGCCA 
GGAGAAGAGA AAGTGGAATT TGAGAAGAAA GCAGCAGCTG AGGCGACACA GGAAACAGTG 
GAGTCCCTGA TGCAGAAGTT CAAGGAGAGT TTCCGTGCTA ACACACCAGT GGAGATCGGT 
CAGTTGCAGC CAGCCTCGCG CAGCAGCACC TCTGCAGGGA AGAGGAAGCG GAGGAACAAA 
TCTCGAGGGG GAATCAGCTT TGGGAGAACC AAGGGGACAT CAGGCTCAGA AGCAGATGAC 
GAAACACAGC TGACCTTCTA CACAGAGCAG TACCGCAGTC GCCGCCGCAG CAAAGGTTTA 
CTGAAAAGCC CTGTGAATAA GACAGCCTTA ACACTGATTG CTGTGAGCTC CTGCATCTTG 
GCCATGGTGT GTGGCAACCA GATGTCCTGT CCACTTACTG TGAAGGTGAC TTTGCATGTG 
CCTGAACACT TCATTGCAGA TGGGAGCAGC TTTGTGGTGA GTGAAGGAAG CTACCTGGAT 
ATCTCCGATT GGCTAAACCC TGCCAAGCTG TCTCTGTATT ACCAGATCAA TGCCACCTCG 
CCATGGGTGA GAGACCTCTG TGGACAGAGG ACGACAGATG CCTGTGAACA GCTCTGTGAC 
CCAGATACTG GGGAGTGCAG TTGTCATGAA GGTTACGCCC CTGACCCAGT CCACAGACAT 
CTATGTGTTC GCAGTGACTG GGGTCAAAGT GAAGGACCTT GGCCTTACAC AACACTGGAG 
AGGGGCTATG ATCTGGTGAC AGGAGAGCAA GCCCCTGAAA AGATTCTCAG GTCTACTTTC 
AGCTTGGGCC AAGGCCTCTG GCTTCCGGTC AGCAAAAGCT TTGTGGTGCC ACCGGTGGAG 
CTGTCCATCA ACCCCCTGGC CAGCTGCAAG ACGGATGTGC TCGTCACAGA AGACCCTGCG 
GATGTCAGGG AAGAAGCAAT GTTGTCCACG TACTTTGAAA CCATCAACGA CCTGCTGTCC 
TCCTTTGGTC CAGTCCGTGA CTGCTCAAGG AACAACGGGG GCTGTACCCG TAACTTCAAG 
TGTGTGTCTG ACCGCCAGGT GGACTCCTCG GGATGTGTGT GTCCTGAGGA ACTGAAGCCC 
ATGAAGGATG GCTCTGGCTG CTATGATCAC TCCAAGGGCA TCGACTGCTC TGATGGCTTT 
AATGGAGGGT GTGAGCAGCT TTGCCTACAA CAAACACTGC CTTTGCCCTA TGATACCACC 
TCCAGCACCA TCTTCATGTT CTGCGGCTGT GTGGAAGAGT ACAAGCTGGC TCCTGATGGG 
AAATCCTGTC TAATGCTCTC AGATGTCTGT GAGGGCCCCA AGTGCCTCAA ACCTGACTCC 
AAATTCAATG ACACCCTCTT TGGAGAGATG CTACATGGTT ACAACAACCG GACACAACAT 
GTAAACCAAG GCCAAGTCTT CCAAATGACA TTCAGGGAGA ATAACTTCAT CAAGGACTTT 
CCACAGCTGG CAGATGGGCT GTTGGTGATC CCGCTGCCAG TGGAAGAGCA ATGCCGGGGG 
GTTCTCTCTG AGCCCCTTCC AGACCTTCAG CTTCTCACAG GAGACATCAG GTATGATGAG 
GCCATGGGGT ACCCCATGGT GCAACAGTGG CGTGTCCGGA GCAATCTCTA TCGTGTGAAG 
CTGAGTACTA TCACCCTCTC AGCAGGCTTC ACCAATGTCC TAAAGATCCT GACCAAAGAG 
AGCAGTCGGG ATGAGCTGCT GTCCTTCATC CAGCACTATG GCTCCCACTA CATTGCCGAG 
GCTTTATACG GTTCTGAGCT CACCTGCATC ATCCATTTTC CCAGCAAGAA GGTCCAGCAG 
CAGCTATGGC TCCAATATCA GAAAGAGACC ACGGAGCTGG GCAGCAAGAA GGAGCTCAAG 
TCCATGCCCT TCATCACCTA CCTCTCCGGT CTCCTGACAG CCCAGATGCT ATCAGATGAC 
CAGCTCATCT CAGGTGTGGA GATTCGATGT GAGGAGAAGG GCCGCTGTCC ATCTACCTGT 
CACCTTTGCC GCCGGCCAGG CAAAGAGCAG CTGAGCCCCA CACCAGTGCT GCTGGAGATA 
AACCGTGTGG TTCCACTTTA TACCCTCATC CAAGACAATG GCACAAAGGA GGCCTTCAAG 
AATGCACTGA TGAGTTCTTA CTGGTGTTCT GGTAAAGGAG ATGTGATTGA TGACTGGTGC 
AGGTGTGACC TCAGTGCCTT TGATGCCAGT GGGCTTCCAA ATTGTAGCCC CCTTCCACAG 
CCGGTGTTAC GACTTTCTCC AACAGTGGAG CCTTCCAGCA CTGTGGTTTC CCTGGAGTGG 
GTAGATGTTC AGCCAGCCAT TGGAACCAAA GTCTCTGACT ATATTCTGCA GCACAAGAAA 
GTGGATGAAT ACACAGACAC GGACCTATAC ACAGGAGAAT TCCTGAGTTT TGCCGATGAC 
TTACTTTCTG GCCTGGGTAC ATCTTGTGTA GCAGCTGGTC GAAGCCATGG AGAGGTTCCT 
GAAGTCAGTA TCTACTCGGT GATCTTCAAG TGCCTGGAGC CAGATGGTCT TTACAAGTTC 
ACTCTGTATG CTGTGGATAC TCGAGGGAGG CACTCAGAGC TCAGCACAGT GACTCTAAGG 
ACAGCTTGTC CACTGGTAGA TGACAACAAA GCAGAAGAGA TAGCGGACAA GATTTACAAT 
CTATACAATG GTTACACAAG TGGCAAGGAG CAGCAGACTG CTTACAACAC ACTGATGGAA 
GTCTCCGCCT CCATGCTCTT CCGTGTCCAG CACCACTACA ACTCTCACTA TGAAAAGTTT 
GGTGACTTCG TCTGGAGGAG TGAGGATGAG CTAGGACCAA GGAAGGCTCA TCTGATTCTG 
CGGCGGCTGG AGCGGGTCAG CAGCCACTGT TCTAGTCTCT TGAGAAGCGC CTACATCCAG 
AGTCGTGTGG ACACCATACC CTATCTCTTC TGCCGCAGTG AGGAGGTCCG GCCTGCAGGA 
ATGGTGTGGT ACAGCATCCT CAAAGACACC AAAATCACAT GTGAGGAGAA GATGGTGTCT 
ATGGCCCGAA ACACGTACGG GGAAACCAAA GGCCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996992.1
CDS	124..4182
Translation	MAAAGARRSPGRGLGLRGRPRLGFHPGPPPPPPPPLLLLFLLLLPPPPLLAGATAAAASREPDSPCRLKTVTVSTLPALRESDIGWSGARTGAAAGAGAGTGAGAGAAAAAASAASPGSAGSAGTAAESRLLLFVRNELPGRIAVQDDLDNTELPFFTLEMSGTAADISLVHWRQQWLENGTLYFHVSMSSSGQLAQATAPTLQEPSEIVEEQMHILHISVMGGLIALLLLLLVFTVALYAQRRWQKRRRIPQKSASTEATHEIHYIPSVLLGPQARESFRSSRLQTHNSVIGVPIRETPILDDYDYEEEEEPPRRANHVSREDEFGSQMTHALDSLGRPGEEKVEFEKKAAAEATQETVESLMQKFKESFRANTPVEIGQLQPASRSSTSAGKRKRRNKSRGGISFGRTKGTSGSEADDETQLTFYTEQYRSRRRSKGLLKSPVNKTALTLIAVSSCILAMVCGNQMSCPLTVKVTLHVPEHFIADGSSFVVSEGSYLDISDWLNPAKLSLYYQINATSPWVRDLCGQRTTDACEQLCDPDTGECSCHEGYAPDPVHRHLCVRSDWGQSEGPWPYTTLERGYDLVTGEQAPEKILRSTFSLGQGLWLPVSKSFVVPPVELSINPLASCKTDVLVTEDPADVREEAMLSTYFETINDLLSSFGPVRDCSRNNGGCTRNFKCVSDRQVDSSGCVCPEELKPMKDGSGCYDHSKGIDCSDGFNGGCEQLCLQQTLPLPYDTTSSTIFMFCGCVEEYKLAPDGKSCLMLSDVCEGPKCLKPDSKFNDTLFGEMLHGYNNRTQHVNQGQVFQMTFRENNFIKDFPQLADGLLVIPLPVEEQCRGVLSEPLPDLQLLTGDIRYDEAMGYPMVQQWRVRSNLYRVKLSTITLSAGFTNVLKILTKESSRDELLSFIQHYGSHYIAEALYGSELTCIIHFPSKKVQQQLWLQYQKETTELGSKKELKSMPFITYLSGLLTAQMLSDDQLISGVEIRCEEKGRCPSTCHLCRRPGKEQLSPTPVLLEINRVVPLYTLIQDNGTKEAFKNALMSSYWCSGKGDVIDDWCRCDLSAFDASGLPNCSPLPQPVLRLSPTVEPSSTVVSLEWVDVQPAIGTKVSDYILQHKKVDEYTDTDLYTGEFLSFADDLLSGLGTSCVAAGRSHGEVPEVSIYSVIFKCLEPDGLYKFTLYAVDTRGRHSELSTVTLRTACPLVDDNKAEEIADKIYNLYNGYTSGKEQQTAYNTLMEVSASMLFRVQHHYNSHYEKFGDFVWRSEDELGPRKAHLILRRLERVSSHCSSLLRSAYIQSRVDTIPYLFCRSEEVRPAGMVWYSILKDTKITCEEKMVSMARNTYGETKGR

Target ORF information:

RefSeq Version	NM_207109.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus astrotactin 2 (Astn2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207109.2

ORF Insert Sequence:

ATGGCCGCCG CCGGCGCCCG GCGCAGCCCG GGCCGCGGCT TGGGGCTTCG GGGGCGGCCG 
AGGCTCGGCT TCCACCCGGG GCCGCCGCCG CCACCGCCGC CGCCGCTGCT GTTGCTGTTT 
CTGCTCCTGC TGCCGCCGCC GCCGTTGCTG GCCGGCGCCA CGGCCGCCGC CGCCTCGCGG 
GAGCCCGACA GCCCATGCCG GCTCAAGACC GTCACCGTAT CCACGCTGCC CGCTCTGCGG 
GAAAGCGACA TCGGCTGGAG CGGTGCCCGC ACCGGGGCCG CGGCCGGGGC TGGGGCCGGG 
ACCGGAGCCG GGGCCGGAGC TGCTGCTGCT GCCGCCTCCG CCGCGTCCCC GGGCTCCGCG 
GGTTCAGCCG GCACAGCCGC GGAGTCTCGT CTCCTTCTCT TTGTGCGTAA TGAGCTCCCC 
GGGCGCATCG CGGTACAAGA TGACCTGGAC AACACTGAGC TGCCCTTCTT CACCCTGGAG 
ATGTCTGGCA CAGCAGCGGA CATTTCTTTG GTTCACTGGA GGCAGCAGTG GCTGGAGAAT 
GGCACATTGT ACTTCCACGT TTCCATGAGC AGCTCTGGGC AACTGGCTCA GGCCACTGCT 
CCCACACTCC AGGAGCCCTC GGAGATCGTT GAGGAACAGA TGCATATCCT CCACATTTCT 
GTGATGGGTG GACTCATTGC ACTTCTCCTC CTGCTGTTGG TGTTCACAGT GGCACTGTAT 
GCCCAGCGAC GCTGGCAGAA GCGCCGGCGC ATCCCACAGA AGAGCGCAAG TACAGAAGCT 
ACTCATGAGA TTCACTACAT CCCATCAGTG CTGCTGGGTC CTCAGGCCAG GGAAAGCTTC 
CGATCCTCCA GACTTCAGAC ACACAACTCA GTCATTGGTG TGCCTATCCG GGAAACCCCC 
ATCCTGGATG ACTATGACTA TGAAGAGGAG GAGGAACCAC CCAGGCGAGC CAACCACGTC 
TCCCGTGAGG ATGAGTTTGG TAGCCAGATG ACCCATGCCC TGGACAGCTT GGGAAGGCCA 
GGAGAAGAGA AAGTGGAATT TGAGAAGAAA GCAGCAGCTG AGGCGACACA GGAAACAGTG 
GAGTCCCTGA TGCAGAAGTT CAAGGAGAGT TTCCGTGCTA ACACACCAGT GGAGATCGGT 
CAGTTGCAGC CAGCCTCGCG CAGCAGCACC TCTGCAGGGA AGAGGAAGCG GAGGAACAAA 
TCTCGAGGGG GAATCAGCTT TGGGAGAACC AAGGGGACAT CAGGCTCAGA AGCAGATGAC 
GAAACACAGC TGACCTTCTA CACAGAGCAG TACCGCAGTC GCCGCCGCAG CAAAGGTTTA 
CTGAAAAGCC CTGTGAATAA GACAGCCTTA ACACTGATTG CTGTGAGCTC CTGCATCTTG 
GCCATGGTGT GTGGCAACCA GATGTCCTGT CCACTTACTG TGAAGGTGAC TTTGCATGTG 
CCTGAACACT TCATTGCAGA TGGGAGCAGC TTTGTGGTGA GTGAAGGAAG CTACCTGGAT 
ATCTCCGATT GGCTAAACCC TGCCAAGCTG TCTCTGTATT ACCAGATCAA TGCCACCTCG 
CCATGGGTGA GAGACCTCTG TGGACAGAGG ACGACAGATG CCTGTGAACA GCTCTGTGAC 
CCAGATACTG GGGAGTGCAG TTGTCATGAA GGTTACGCCC CTGACCCAGT CCACAGACAT 
CTATGTGTTC GCAGTGACTG GGGTCAAAGT GAAGGACCTT GGCCTTACAC AACACTGGAG 
AGGGGCTATG ATCTGGTGAC AGGAGAGCAA GCCCCTGAAA AGATTCTCAG GTCTACTTTC 
AGCTTGGGCC AAGGCCTCTG GCTTCCGGTC AGCAAAAGCT TTGTGGTGCC ACCGGTGGAG 
CTGTCCATCA ACCCCCTGGC CAGCTGCAAG ACGGATGTGC TCGTCACAGA AGACCCTGCG 
GATGTCAGGG AAGAAGCAAT GTTGTCCACG TACTTTGAAA CCATCAACGA CCTGCTGTCC 
TCCTTTGGTC CAGTCCGTGA CTGCTCAAGG AACAACGGGG GCTGTACCCG TAACTTCAAG 
TGTGTGTCTG ACCGCCAGGT GGACTCCTCG GGATGTGTGT GTCCTGAGGA ACTGAAGCCC 
ATGAAGGATG GCTCTGGCTG CTATGATCAC TCCAAGGGCA TCGACTGCTC TGATGGCTTT 
AATGGAGGGT GTGAGCAGCT TTGCCTACAA CAAACACTGC CTTTGCCCTA TGATACCACC 
TCCAGCACCA TCTTCATGTT CTGCGGCTGT GTGGAAGAGT ACAAGCTGGC TCCTGATGGG 
AAATCCTGTC TAATGCTCTC AGATGTCTGT GAGGGCCCCA AGTGCCTCAA ACCTGACTCC 
AAATTCAATG ACACCCTCTT TGGAGAGATG CTACATGGTT ACAACAACCG GACACAACAT 
GTAAACCAAG GCCAAGTCTT CCAAATGACA TTCAGGGAGA ATAACTTCAT CAAGGACTTT 
CCACAGCTGG CAGATGGGCT GTTGGTGATC CCGCTGCCAG TGGAAGAGCA ATGCCGGGGG 
GTTCTCTCTG AGCCCCTTCC AGACCTTCAG CTTCTCACAG GAGACATCAG GTATGATGAG 
GCCATGGGGT ACCCCATGGT GCAACAGTGG CGTGTCCGGA GCAATCTCTA TCGTGTGAAG 
CTGAGTACTA TCACCCTCTC AGCAGGCTTC ACCAATGTCC TAAAGATCCT GACCAAAGAG 
AGCAGTCGGG ATGAGCTGCT GTCCTTCATC CAGCACTATG GCTCCCACTA CATTGCCGAG 
GCTTTATACG GTTCTGAGCT CACCTGCATC ATCCATTTTC CCAGCAAGAA GGTCCAGCAG 
CAGCTATGGC TCCAATATCA GAAAGAGACC ACGGAGCTGG GCAGCAAGAA GGAGCTCAAG 
TCCATGCCCT TCATCACCTA CCTCTCCGGT CTCCTGACAG CCCAGATGCT ATCAGATGAC 
CAGCTCATCT CAGGTGTGGA GATTCGATGT GAGGAGAAGG GCCGCTGTCC ATCTACCTGT 
CACCTTTGCC GCCGGCCAGG CAAAGAGCAG CTGAGCCCCA CACCAGTGCT GCTGGAGATA 
AACCGTGTGG TTCCACTTTA TACCCTCATC CAAGACAATG GCACAAAGGA GGCCTTCAAG 
AATGCACTGA TGAGTTCTTA CTGGTGTTCT GGTAAAGGAG ATGTGATTGA TGACTGGTGC 
AGGTGTGACC TCAGTGCCTT TGATGCCAGT GGGCTTCCAA ATTGTAGCCC CCTTCCACAG 
CCGGTGTTAC GACTTTCTCC AACAGTGGAG CCTTCCAGCA CTGTGGTTTC CCTGGAGTGG 
GTAGATGTTC AGCCAGCCAT TGGAACCAAA GTCTCTGACT ATATTCTGCA GCACAAGAAA 
GTGGATGAAT ACACAGACAC GGACCTATAC ACAGGAGAAT TCCTGAGTTT TGCCGATGAC 
TTACTTTCTG GCCTGGGTAC ATCTTGTGTA GCAGCTGGTC GAAGCCATGG AGAGGTTCCT 
GAAGTCAGTA TCTACTCGGT GATCTTCAAG TGCCTGGAGC CAGATGGTCT TTACAAGTTC 
ACTCTGTATG CTGTGGATAC TCGAGGGAGG CACTCAGAGC TCAGCACAGT GACTCTAAGG 
ACAGCTTGTC CACTGGTAGA TGACAACAAA GCAGAAGAGA TAGCGGACAA GATTTACAAT 
CTATACAATG GTTACACAAG TGGCAAGGAG CAGCAGACTG CTTACAACAC ACTGATGGAA 
GTCTCCGCCT CCATGCTCTT CCGTGTCCAG CACCACTACA ACTCTCACTA TGAAAAGTTT 
GGTGACTTCG TCTGGAGGAG TGAGGATGAG CTAGGACCAA GGAAGGCTCA TCTGATTCTG 
CGGCGGCTGG AGCGGGTCAG CAGCCACTGT TCTAGTCTCT TGAGAAGCGC CTACATCCAG 
AGTCGTGTGG ACACCATACC CTATCTCTTC TGCCGCAGTG AGGAGGTCCG GCCTGCAGGA 
ATGGTGTGGT ACAGCATCCT CAAAGACACC AAAATCACAT GTGAGGAGAA GATGGTGTCT 
ATGGCCCGAA ACACGTACGG GGAAACCAAA GGCCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Astn2 ( XM_006538104.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538167.1 Mus musculus astrotactin 2 (Astn2), transcript variant X1, mRNA.

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CloneID	OMu33057
Clone ID Related Accession (Same CDS sequence)	XM_006538104.3
Accession Version	XM_006538104.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	astrotactin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538104.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCG CCGGCGCCCG GCGCAGCCCG GGCCGCGGCT TGGGGCTTCG GGGGCGGCCG 
AGGCTCGGCT TCCACCCGGG GCCGCCGCCG CCACCGCCGC CGCCGCTGCT GTTGCTGTTT 
CTGCTCCTGC TGCCGCCGCC GCCGTTGCTG GCCGGCGCCA CGGCCGCCGC CGCCTCGCGG 
GAGCCCGACA GCCCATGCCG GCTCAAGACC GTCACCGTAT CCACGCTGCC CGCTCTGCGG 
GAAAGCGACA TCGGCTGGAG CGGTGCCCGC ACCGGGGCCG CGGCCGGGGC TGGGGCCGGG 
ACCGGAGCCG GGGCCGGAGC TGCTGCTGCT GCCGCCTCCG CCGCGTCCCC GGGCTCCGCG 
GGTTCAGCCG GCACAGCCGC GGAGTCTCGT CTCCTTCTCT TTGTGCGTAA TGAGCTCCCC 
GGGCGCATCG CGGTACAAGA TGACCTGGAC AACACTGAGC TGCCCTTCTT CACCCTGGAG 
ATGTCTGGCA CAGCAGCGGA CATTTCTTTG GTTCACTGGA GGCAGCAGTG GCTGGAGAAT 
GGCACATTGT ACTTCCACGT TTCCATGAGC AGCTCTGGGC AACTGGCTCA GGCCACTGCT 
CCCACACTCC AGGAGCCCTC GGAGATCGTT GAGGAACAGA TGCATATCCT CCACATTTCT 
GTGATGGGTG GACTCATTGC ACTTCTCCTC CTGCTGTTGG TGTTCACAGT GGCACTGTAT 
GCCCAGCGAC GCTGGCAGAA GCGCCGGCGC ATCCCACAGA AGAGCGCAAG TACAGAAGCT 
ACTCATGAGA TTCACTACAT CCCATCAGTG CTGCTGGGTC CTCAGGCCAG GGAAAGCTTC 
CGATCCTCCA GACTTCAGAC ACACAACTCA GTCATTGGTG TGCCTATCCG GGAAACCCCC 
ATCCTGGATG ACTATGACTA TGAAGAGGAG GAGGAACCAC CCAGGCGAGC CAACCACGTC 
TCCCGTGAGG ATGAGTTTGG TAGCCAGATG ACCCATGCCC TGGACAGCTT GGGAAGGCCA 
GGAGAAGAGA AAGTGGAATT TGAGAAGAAA GCAGCAGCTG AGGCGACACA GGAAACAGTG 
GAGTCCCTGA TGCAGAAGTT CAAGGAGAGT TTCCGTGCTA ACACACCAGT GGAGATCGGT 
CAGTTGCAGC CAGCCTCGCG CAGCAGCACC TCTGCAGGGA AGAGGAAGCG GAGGAACAAA 
TCTCGAGGGG GAATCAGCTT TGGGAGAACC AAGGGGACAT CAGGCTCAGA AGCAGATGAC 
GAAACACAGC TGACCTTCTA CACAGAGCAG TACCGCAGTC GCCGCCGCAG CAAAGGTTTA 
CTGAAAAGCC CTGTGAATAA GACAGCCTTA ACACTGATTG CTGTGAGCTC CTGCATCTTG 
GCCATGGTGT GTGGCAACCA GATGTCCTGT CCACTTACTG TGAAGGTGAC TTTGCATGTG 
CCTGAACACT TCATTGCAGA TGGGAGCAGC TTTGTGGTGA GTGAAGGAAG CTACCTGGAT 
ATCTCCGATT GGCTAAACCC TGCCAAGCTG TCTCTGTATT ACCAGATCAA TGCCACCTCG 
CCATGGGTGA GAGACCTCTG TGGACAGAGG ACGACAGATG CCTGTGAACA GCTCTGTGAC 
CCAGATACTG GGGAGTGCAG TTGTCATGAA GGTTACGCCC CTGACCCAGT CCACAGACAT 
CTATGTGTTC GCAGTGACTG GGGTCAAAGT GAAGGACCTT GGCCTTACAC AACACTGGAG 
AGGGGCTATG ATCTGGTGAC AGGAGAGCAA GCCCCTGAAA AGATTCTCAG CTTGGGCCAA 
GGCCTCTGGC TTCCGGTCAG CAAAAGCTTT GTGGTGCCAC CGGTGGAGCT GTCCATCAAC 
CCCCTGGCCA GCTGCAAGAC GGATGTGCTC GTCACAGAAG ACCCTGCGGA TGTCAGGGAA 
GAAGCAATGT TGTCCACGTA CTTTGAAACC ATCAACGACC TGCTGTCCTC CTTTGGTCCA 
GTCCGTGACT GCTCAAGGAA CAACGGGGGC TGTACCCGTA ACTTCAAGTG TGTGTCTGAC 
CGCCAGGTGG ACTCCTCGGG ATGTGTGTGT CCTGAGGAAC TGAAGCCCAT GAAGGATGGC 
TCTGGCTGCT ATGATCACTC CAAGGGCATC GACTGCTCTG ATGGCTTTAA TGGAGGGTGT 
GAGCAGCTTT GCCTACAACA AACACTGCCT TTGCCCTATG ATACCACCTC CAGCACCATC 
TTCATGTTCT GCGGCTGTGT GGAAGAGTAC AAGCTGGCTC CTGATGGGAA ATCCTGTCTA 
ATGCTCTCAG ATGTCTGTGA GGGCCCCAAG TGCCTCAAAC CTGACTCCAA ATTCAATGAC 
ACCCTCTTTG GAGAGATGCT ACATGGTTAC AACAACCGGA CACAACATGT AAACCAAGGC 
CAAGTCTTCC AAATGACATT CAGGGAGAAT AACTTCATCA AGGACTTTCC ACAGCTGGCA 
GATGGGCTGT TGGTGATCCC GCTGCCAGTG GAAGAGCAAT GCCGGGGGGT TCTCTCTGAG 
CCCCTTCCAG ACCTTCAGCT TCTCACAGGA GACATCAGGT ATGATGAGGC CATGGGGTAC 
CCCATGGTGC AACAGTGGCG TGTCCGGAGC AATCTCTATC GTGTGAAGCT GAGTACTATC 
ACCCTCTCAG CAGGCTTCAC CAATGTCCTA AAGATCCTGA CCAAAGAGAG CAGTCGGGAT 
GAGCTGCTGT CCTTCATCCA GCACTATGGC TCCCACTACA TTGCCGAGGC TTTATACGGT 
TCTGAGCTCA CCTGCATCAT CCATTTTCCC AGCAAGAAGG TCCAGCAGCA GCTATGGCTC 
CAATATCAGA AAGAGACCAC GGAGCTGGGC AGCAAGAAGG AGCTCAAGTC CATGCCCTTC 
ATCACCTACC TCTCCGGTCT CCTGACAGCC CAGATGCTAT CAGATGACCA GCTCATCTCA 
GGTGTGGAGA TTCGATGTGA GGAGAAGGGC CGCTGTCCAT CTACCTGTCA CCTTTGCCGC 
CGGCCAGGCA AAGAGCAGCT GAGCCCCACA CCAGTGCTGC TGGAGATAAA CCGTGTGGTT 
CCACTTTATA CCCTCATCCA AGACAATGGC ACAAAGGAGG CCTTCAAGAA TGCACTGATG 
AGTTCTTACT GGTGTTCTGG TAAAGGAGAT GTGATTGATG ACTGGTGCAG GTGTGACCTC 
AGTGCCTTTG ATGCCAGTGG GCTTCCAAAT TGTAGCCCCC TTCCACAGCC GGTGTTACGA 
CTTTCTCCAA CAGTGGAGCC TTCCAGCACT GTGGTTTCCC TGGAGTGGGT AGATGTTCAG 
CCAGCCATTG GAACCAAAGT CTCTGACTAT ATTCTGCAGC ACAAGAAAGT GGATGAATAC 
ACAGACACGG ACCTATACAC AGGAGAATTC CTGAGTTTTG CCGATGACTT ACTTTCTGGC 
CTGGGTACAT CTTGTGTAGC AGCTGGTCGA AGCCATGGAG AGGTTCCTGA AGTCAGTATC 
TACTCGGTGA TCTTCAAGTG CCTGGAGCCA GATGGTCTTT ACAAGTTCAC TCTGTATGCT 
GTGGATACTC GAGGGAGGCA CTCAGAGCTC AGCACAGTGA CTCTAAGGAC AGCTTGTCCA 
CTGGTAGATG ACAACAAAGC AGAAGAGATA GCGGACAAGA TTTACAATCT ATACAATGGT 
TACACAAGTG GCAAGGAGCA GCAGACTGCT TACAACACAC TGATGGAAGT CTCCGCCTCC 
ATGCTCTTCC GTGTCCAGCA CCACTACAAC TCTCACTATG AAAAGTTTGG TGACTTCGTC 
TGGAGGAGTG AGGATGAGCT AGGACCAAGG AAGGCTCATC TGATTCTGCG GCGGCTGGAG 
CGGGTCAGCA GCCACTGTTC TAGTCTCTTG AGAAGCGCCT ACATCCAGAG TCGTGTGGAC 
ACCATACCCT ATCTCTTCTG CCGCAGTGAG GAGGTCCGGC CTGCAGGAAT GGTGTGGTAC 
AGCATCCTCA AAGACACCAA AATCACATGT GAGGAGAAGA TGGTGTCTAT GGCCCGAAAC 
ACGTACGGGG AAACCAAAGG CCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538167.1
CDS	171..4217
Translation	MAAAGARRSPGRGLGLRGRPRLGFHPGPPPPPPPPLLLLFLLLLPPPPLLAGATAAAASREPDSPCRLKTVTVSTLPALRESDIGWSGARTGAAAGAGAGTGAGAGAAAAAASAASPGSAGSAGTAAESRLLLFVRNELPGRIAVQDDLDNTELPFFTLEMSGTAADISLVHWRQQWLENGTLYFHVSMSSSGQLAQATAPTLQEPSEIVEEQMHILHISVMGGLIALLLLLLVFTVALYAQRRWQKRRRIPQKSASTEATHEIHYIPSVLLGPQARESFRSSRLQTHNSVIGVPIRETPILDDYDYEEEEEPPRRANHVSREDEFGSQMTHALDSLGRPGEEKVEFEKKAAAEATQETVESLMQKFKESFRANTPVEIGQLQPASRSSTSAGKRKRRNKSRGGISFGRTKGTSGSEADDETQLTFYTEQYRSRRRSKGLLKSPVNKTALTLIAVSSCILAMVCGNQMSCPLTVKVTLHVPEHFIADGSSFVVSEGSYLDISDWLNPAKLSLYYQINATSPWVRDLCGQRTTDACEQLCDPDTGECSCHEGYAPDPVHRHLCVRSDWGQSEGPWPYTTLERGYDLVTGEQAPEKILSLGQGLWLPVSKSFVVPPVELSINPLASCKTDVLVTEDPADVREEAMLSTYFETINDLLSSFGPVRDCSRNNGGCTRNFKCVSDRQVDSSGCVCPEELKPMKDGSGCYDHSKGIDCSDGFNGGCEQLCLQQTLPLPYDTTSSTIFMFCGCVEEYKLAPDGKSCLMLSDVCEGPKCLKPDSKFNDTLFGEMLHGYNNRTQHVNQGQVFQMTFRENNFIKDFPQLADGLLVIPLPVEEQCRGVLSEPLPDLQLLTGDIRYDEAMGYPMVQQWRVRSNLYRVKLSTITLSAGFTNVLKILTKESSRDELLSFIQHYGSHYIAEALYGSELTCIIHFPSKKVQQQLWLQYQKETTELGSKKELKSMPFITYLSGLLTAQMLSDDQLISGVEIRCEEKGRCPSTCHLCRRPGKEQLSPTPVLLEINRVVPLYTLIQDNGTKEAFKNALMSSYWCSGKGDVIDDWCRCDLSAFDASGLPNCSPLPQPVLRLSPTVEPSSTVVSLEWVDVQPAIGTKVSDYILQHKKVDEYTDTDLYTGEFLSFADDLLSGLGTSCVAAGRSHGEVPEVSIYSVIFKCLEPDGLYKFTLYAVDTRGRHSELSTVTLRTACPLVDDNKAEEIADKIYNLYNGYTSGKEQQTAYNTLMEVSASMLFRVQHHYNSHYEKFGDFVWRSEDELGPRKAHLILRRLERVSSHCSSLLRSAYIQSRVDTIPYLFCRSEEVRPAGMVWYSILKDTKITCEEKMVSMARNTYGETKGR

Target ORF information:

RefSeq Version	XM_006538104.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus astrotactin 2 (Astn2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538104.3

ORF Insert Sequence:

ATGGCCGCCG CCGGCGCCCG GCGCAGCCCG GGCCGCGGCT TGGGGCTTCG GGGGCGGCCG 
AGGCTCGGCT TCCACCCGGG GCCGCCGCCG CCACCGCCGC CGCCGCTGCT GTTGCTGTTT 
CTGCTCCTGC TGCCGCCGCC GCCGTTGCTG GCCGGCGCCA CGGCCGCCGC CGCCTCGCGG 
GAGCCCGACA GCCCATGCCG GCTCAAGACC GTCACCGTAT CCACGCTGCC CGCTCTGCGG 
GAAAGCGACA TCGGCTGGAG CGGTGCCCGC ACCGGGGCCG CGGCCGGGGC TGGGGCCGGG 
ACCGGAGCCG GGGCCGGAGC TGCTGCTGCT GCCGCCTCCG CCGCGTCCCC GGGCTCCGCG 
GGTTCAGCCG GCACAGCCGC GGAGTCTCGT CTCCTTCTCT TTGTGCGTAA TGAGCTCCCC 
GGGCGCATCG CGGTACAAGA TGACCTGGAC AACACTGAGC TGCCCTTCTT CACCCTGGAG 
ATGTCTGGCA CAGCAGCGGA CATTTCTTTG GTTCACTGGA GGCAGCAGTG GCTGGAGAAT 
GGCACATTGT ACTTCCACGT TTCCATGAGC AGCTCTGGGC AACTGGCTCA GGCCACTGCT 
CCCACACTCC AGGAGCCCTC GGAGATCGTT GAGGAACAGA TGCATATCCT CCACATTTCT 
GTGATGGGTG GACTCATTGC ACTTCTCCTC CTGCTGTTGG TGTTCACAGT GGCACTGTAT 
GCCCAGCGAC GCTGGCAGAA GCGCCGGCGC ATCCCACAGA AGAGCGCAAG TACAGAAGCT 
ACTCATGAGA TTCACTACAT CCCATCAGTG CTGCTGGGTC CTCAGGCCAG GGAAAGCTTC 
CGATCCTCCA GACTTCAGAC ACACAACTCA GTCATTGGTG TGCCTATCCG GGAAACCCCC 
ATCCTGGATG ACTATGACTA TGAAGAGGAG GAGGAACCAC CCAGGCGAGC CAACCACGTC 
TCCCGTGAGG ATGAGTTTGG TAGCCAGATG ACCCATGCCC TGGACAGCTT GGGAAGGCCA 
GGAGAAGAGA AAGTGGAATT TGAGAAGAAA GCAGCAGCTG AGGCGACACA GGAAACAGTG 
GAGTCCCTGA TGCAGAAGTT CAAGGAGAGT TTCCGTGCTA ACACACCAGT GGAGATCGGT 
CAGTTGCAGC CAGCCTCGCG CAGCAGCACC TCTGCAGGGA AGAGGAAGCG GAGGAACAAA 
TCTCGAGGGG GAATCAGCTT TGGGAGAACC AAGGGGACAT CAGGCTCAGA AGCAGATGAC 
GAAACACAGC TGACCTTCTA CACAGAGCAG TACCGCAGTC GCCGCCGCAG CAAAGGTTTA 
CTGAAAAGCC CTGTGAATAA GACAGCCTTA ACACTGATTG CTGTGAGCTC CTGCATCTTG 
GCCATGGTGT GTGGCAACCA GATGTCCTGT CCACTTACTG TGAAGGTGAC TTTGCATGTG 
CCTGAACACT TCATTGCAGA TGGGAGCAGC TTTGTGGTGA GTGAAGGAAG CTACCTGGAT 
ATCTCCGATT GGCTAAACCC TGCCAAGCTG TCTCTGTATT ACCAGATCAA TGCCACCTCG 
CCATGGGTGA GAGACCTCTG TGGACAGAGG ACGACAGATG CCTGTGAACA GCTCTGTGAC 
CCAGATACTG GGGAGTGCAG TTGTCATGAA GGTTACGCCC CTGACCCAGT CCACAGACAT 
CTATGTGTTC GCAGTGACTG GGGTCAAAGT GAAGGACCTT GGCCTTACAC AACACTGGAG 
AGGGGCTATG ATCTGGTGAC AGGAGAGCAA GCCCCTGAAA AGATTCTCAG CTTGGGCCAA 
GGCCTCTGGC TTCCGGTCAG CAAAAGCTTT GTGGTGCCAC CGGTGGAGCT GTCCATCAAC 
CCCCTGGCCA GCTGCAAGAC GGATGTGCTC GTCACAGAAG ACCCTGCGGA TGTCAGGGAA 
GAAGCAATGT TGTCCACGTA CTTTGAAACC ATCAACGACC TGCTGTCCTC CTTTGGTCCA 
GTCCGTGACT GCTCAAGGAA CAACGGGGGC TGTACCCGTA ACTTCAAGTG TGTGTCTGAC 
CGCCAGGTGG ACTCCTCGGG ATGTGTGTGT CCTGAGGAAC TGAAGCCCAT GAAGGATGGC 
TCTGGCTGCT ATGATCACTC CAAGGGCATC GACTGCTCTG ATGGCTTTAA TGGAGGGTGT 
GAGCAGCTTT GCCTACAACA AACACTGCCT TTGCCCTATG ATACCACCTC CAGCACCATC 
TTCATGTTCT GCGGCTGTGT GGAAGAGTAC AAGCTGGCTC CTGATGGGAA ATCCTGTCTA 
ATGCTCTCAG ATGTCTGTGA GGGCCCCAAG TGCCTCAAAC CTGACTCCAA ATTCAATGAC 
ACCCTCTTTG GAGAGATGCT ACATGGTTAC AACAACCGGA CACAACATGT AAACCAAGGC 
CAAGTCTTCC AAATGACATT CAGGGAGAAT AACTTCATCA AGGACTTTCC ACAGCTGGCA 
GATGGGCTGT TGGTGATCCC GCTGCCAGTG GAAGAGCAAT GCCGGGGGGT TCTCTCTGAG 
CCCCTTCCAG ACCTTCAGCT TCTCACAGGA GACATCAGGT ATGATGAGGC CATGGGGTAC 
CCCATGGTGC AACAGTGGCG TGTCCGGAGC AATCTCTATC GTGTGAAGCT GAGTACTATC 
ACCCTCTCAG CAGGCTTCAC CAATGTCCTA AAGATCCTGA CCAAAGAGAG CAGTCGGGAT 
GAGCTGCTGT CCTTCATCCA GCACTATGGC TCCCACTACA TTGCCGAGGC TTTATACGGT 
TCTGAGCTCA CCTGCATCAT CCATTTTCCC AGCAAGAAGG TCCAGCAGCA GCTATGGCTC 
CAATATCAGA AAGAGACCAC GGAGCTGGGC AGCAAGAAGG AGCTCAAGTC CATGCCCTTC 
ATCACCTACC TCTCCGGTCT CCTGACAGCC CAGATGCTAT CAGATGACCA GCTCATCTCA 
GGTGTGGAGA TTCGATGTGA GGAGAAGGGC CGCTGTCCAT CTACCTGTCA CCTTTGCCGC 
CGGCCAGGCA AAGAGCAGCT GAGCCCCACA CCAGTGCTGC TGGAGATAAA CCGTGTGGTT 
CCACTTTATA CCCTCATCCA AGACAATGGC ACAAAGGAGG CCTTCAAGAA TGCACTGATG 
AGTTCTTACT GGTGTTCTGG TAAAGGAGAT GTGATTGATG ACTGGTGCAG GTGTGACCTC 
AGTGCCTTTG ATGCCAGTGG GCTTCCAAAT TGTAGCCCCC TTCCACAGCC GGTGTTACGA 
CTTTCTCCAA CAGTGGAGCC TTCCAGCACT GTGGTTTCCC TGGAGTGGGT AGATGTTCAG 
CCAGCCATTG GAACCAAAGT CTCTGACTAT ATTCTGCAGC ACAAGAAAGT GGATGAATAC 
ACAGACACGG ACCTATACAC AGGAGAATTC CTGAGTTTTG CCGATGACTT ACTTTCTGGC 
CTGGGTACAT CTTGTGTAGC AGCTGGTCGA AGCCATGGAG AGGTTCCTGA AGTCAGTATC 
TACTCGGTGA TCTTCAAGTG CCTGGAGCCA GATGGTCTTT ACAAGTTCAC TCTGTATGCT 
GTGGATACTC GAGGGAGGCA CTCAGAGCTC AGCACAGTGA CTCTAAGGAC AGCTTGTCCA 
CTGGTAGATG ACAACAAAGC AGAAGAGATA GCGGACAAGA TTTACAATCT ATACAATGGT 
TACACAAGTG GCAAGGAGCA GCAGACTGCT TACAACACAC TGATGGAAGT CTCCGCCTCC 
ATGCTCTTCC GTGTCCAGCA CCACTACAAC TCTCACTATG AAAAGTTTGG TGACTTCGTC 
TGGAGGAGTG AGGATGAGCT AGGACCAAGG AAGGCTCATC TGATTCTGCG GCGGCTGGAG 
CGGGTCAGCA GCCACTGTTC TAGTCTCTTG AGAAGCGCCT ACATCCAGAG TCGTGTGGAC 
ACCATACCCT ATCTCTTCTG CCGCAGTGAG GAGGTCCGGC CTGCAGGAAT GGTGTGGTAC 
AGCATCCTCA AAGACACCAA AATCACATGT GAGGAGAAGA TGGTGTCTAT GGCCCGAAAC 
ACGTACGGGG AAACCAAAGG CCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Astn2 ( XM_006538105.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538168.1 Mus musculus astrotactin 2 (Astn2), transcript variant X2, mRNA.

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CloneID	OMu33058
Clone ID Related Accession (Same CDS sequence)	XM_006538105.4 , XM_006538105.3
Accession Version	XM_006538105.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	astrotactin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538105.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCG CCGGCGCCCG GCGCAGCCCG GGCCGCGGCT TGGGGCTTCG GGGGCGGCCG 
AGGCTCGGCT TCCACCCGGG GCCGCCGCCG CCACCGCCGC CGCCGCTGCT GTTGCTGTTT 
CTGCTCCTGC TGCCGCCGCC GCCGTTGCTG GCCGGCGCCA CGGCCGCCGC CGCCTCGCGG 
GAGCCCGACA GCCCATGCCG GCTCAAGACC GTCACCGTAT CCACGCTGCC CGCTCTGCGG 
GAAAGCGACA TCGGCTGGAG CGGTGCCCGC ACCGGGGCCG CGGCCGGGGC TGGGGCCGGG 
ACCGGAGCCG GGGCCGGAGC TGCTGCTGCT GCCGCCTCCG CCGCGTCCCC GGGCTCCGCG 
GGTTCAGCCG GCACAGCCGC GGAGTCTCGT CTCCTTCTCT TTGTGCGTAA TGAGCTCCCC 
GGGCGCATCG CGGTACAAGA TGACCTGGAC AACACTGAGC TGCCCTTCTT CACCCTGGAG 
ATGTCTGGCA CAGCAGCGGA CATTTCTTTG GTTCACTGGA GGCAGCAGTG GCTGGAGAAT 
GGCACATTGT ACTTCCACGT TTCCATGAGC AGCTCTGGGC AACTGGCTCA GGCCACTGCT 
CCCACACTCC AGGAGCCCTC GGAGATCGTT GAGGAACAGA TGCATATCCT CCACATTTCT 
GTGATGGGTG GACTCATTGC ACTTCTCCTC CTGCTGTTGG TGTTCACAGT GGCACTGTAT 
GCCCAGCGAC GCTGGCAGAA GCGCCGGCGC ATCCCACAGA AGAGCGCAAG TACAGAAGCT 
ACTCATGAGA TTCACTACAT CCCATCAGTG CTGCTGGGTC CTCAGGCCAG GGAAAGCTTC 
CGATCCTCCA GACTTCAGAC ACACAACTCA GTCATTGGTG TGCCTATCCG GGAAACCCCC 
ATCCTGGATG ACTATGACTA TGAAGAGGAG GAGGAACCAC CCAGGCGAGC CAACCACGTC 
TCCCGTGAGG ATGAGTTTGG TAGCCAGATG ACCCATGCCC TGGACAGCTT GGGAAGGCCA 
GGAGAAGAGA AAGTGGAATT TGAGAAGAAA GCAGCAGCTG AGGCGACACA GGAAACAGTG 
GAGTCCCTGA TGCAGAAGTT CAAGGAGAGT TTCCGTGCTA ACACACCAGT GGAGATCGGT 
CAGTTGCAGC CAGCCTCGCG CAGCAGCACC TCTGCAGGGA AGAGGAAGCG GAGGAACAAA 
TCTCGAGGGG GAATCAGCTT TGGGAGAACC AAGGGGACAT CAGGCTCAGA AGCAGATGAC 
GAAACACAGC TGACCTTCTA CACAGAGCAG TACCGCAGTC GCCGCCGCAG CAAAGGTTTA 
CTGAAAAGCC CTGTGAATAA GACAGCCTTA ACACTGATTG CTGTGAGCTC CTGCATCTTG 
GCCATGGTGT GTGGCAACCA GATGTCCTGT CCACTTACTG TGAAGGTGAC TTTGCATGTG 
CCTGAACACT TCATTGCAGA TGGGAGCAGC TTTGTGGTGA GTGAAGGAAG CTACCTGGAT 
ATCTCCGATT GGCTAAACCC TGCCAAGCTG TCTCTGTATT ACCAGATCAA TGCCACCTCG 
CCATGGGTGA GAGACCTCTG TGGACAGAGG ACGACAGATG CCTGTGAACA GCTCTGTGAC 
CCAGATACTG GGGAGTGCAG TTGTCATGAA GGTTACGCCC CTGACCCAGT CCACAGACAT 
CTATGTGTTC GCAGTGACTG GGGTCAAAGT GAAGGACCTT GGCCTTACAC AACACTGGAG 
AGGGGCTATG ATCTGGTGAC AGGAGAGCAA GCCCCTGAAA AGATTCTCAG GTCTACTTTC 
AGCTTGGGCC AAGGCCTCTG GCTTCCGGTC AGCAAAAGCT TTGTGGTGCC ACCGGTGGAG 
CTGTCCATCA ACCCCCTGGC CAGCTGCAAG ACGGATGTGC TCGTCACAGA AGACCCTGCG 
GATGTCAGGG AAGAAGCAAT GTTGTCCACG TACTTTGAAA CCATCAACGA CCTGCTGTCC 
TCCTTTGGTC CAGTCCGTGA CTGCTCAAGG AACAACGGGG GCTGTACCCG TAACTTCAAG 
TGTGTGTCTG ACCGCCAGGT GGACTCCTCG GGATGTGTGT GTCCTGAGGA ACTGAAGCCC 
ATGAAGGATG GCTCTGGCTG CTATGATCAC TCCAAGGGCA TCGACTGCTC TGATGGCTTT 
AATGGAGGGT GTGAGCAGCT TTGCCTACAA CAAACACTGC CTTTGCCCTA TGATACCACC 
TCCAGCACCA TCTTCATGTT CTGCGGCTGT GTGGAAGAGT ACAAGCTGGC TCCTGATGGG 
AAATCCTGTC TAATGCTCTC AGATGTCTGT GAGGGCCCCA AGTGCCTCAA ACCTGACTCC 
AAATTCAATG ACACCCTCTT TGGAGAGATG CTACATGGTT ACAACAACCG GACACAACAT 
GTAAACCAAG GCCAAGTCTT CCAAATGACA TTCAGGGAGA ATAACTTCAT CAAGGACTTT 
CCACAGCTGG CAGATGGGCT GTTGGTGATC CCGCTGCCAG TGGAAGAGCA ATGCCGGGGG 
GTTCTCTCTG AGCCCCTTCC AGACCTTCAG CTTCTCACAG GAGACATCAG GTATGATGAG 
GCCATGGGGT ACCCCATGGT GCAACAGTGG CGTGTCCGGA GCAATCTCTA TCGTGTGAAG 
CTGAGTACTA TCACCCTCTC AGCAGGCTTC ACCAATGTCC TAAAGATCCT GACCAAAGAG 
AGCAGTCGGG ATGAGCTGCT GTCCTTCATC CAGCACTATG GCTCCCACTA CATTGCCGAG 
GCTTTATACG GTTCTGAGCT CACCTGCATC ATCCATTTTC CCAGCAAGAA GGTCCAGCAG 
CAGCTATGGC TCCAATATCA GAAAGGTGTA AAGGCCCAAG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538168.1
CDS	172..3036
Translation	MAAAGARRSPGRGLGLRGRPRLGFHPGPPPPPPPPLLLLFLLLLPPPPLLAGATAAAASREPDSPCRLKTVTVSTLPALRESDIGWSGARTGAAAGAGAGTGAGAGAAAAAASAASPGSAGSAGTAAESRLLLFVRNELPGRIAVQDDLDNTELPFFTLEMSGTAADISLVHWRQQWLENGTLYFHVSMSSSGQLAQATAPTLQEPSEIVEEQMHILHISVMGGLIALLLLLLVFTVALYAQRRWQKRRRIPQKSASTEATHEIHYIPSVLLGPQARESFRSSRLQTHNSVIGVPIRETPILDDYDYEEEEEPPRRANHVSREDEFGSQMTHALDSLGRPGEEKVEFEKKAAAEATQETVESLMQKFKESFRANTPVEIGQLQPASRSSTSAGKRKRRNKSRGGISFGRTKGTSGSEADDETQLTFYTEQYRSRRRSKGLLKSPVNKTALTLIAVSSCILAMVCGNQMSCPLTVKVTLHVPEHFIADGSSFVVSEGSYLDISDWLNPAKLSLYYQINATSPWVRDLCGQRTTDACEQLCDPDTGECSCHEGYAPDPVHRHLCVRSDWGQSEGPWPYTTLERGYDLVTGEQAPEKILRSTFSLGQGLWLPVSKSFVVPPVELSINPLASCKTDVLVTEDPADVREEAMLSTYFETINDLLSSFGPVRDCSRNNGGCTRNFKCVSDRQVDSSGCVCPEELKPMKDGSGCYDHSKGIDCSDGFNGGCEQLCLQQTLPLPYDTTSSTIFMFCGCVEEYKLAPDGKSCLMLSDVCEGPKCLKPDSKFNDTLFGEMLHGYNNRTQHVNQGQVFQMTFRENNFIKDFPQLADGLLVIPLPVEEQCRGVLSEPLPDLQLLTGDIRYDEAMGYPMVQQWRVRSNLYRVKLSTITLSAGFTNVLKILTKESSRDELLSFIQHYGSHYIAEALYGSELTCIIHFPSKKVQQQLWLQYQKGVKAQG

Target ORF information:

RefSeq Version	XM_006538105.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus astrotactin 2 (Astn2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538105.3

ORF Insert Sequence:

ATGGCCGCCG CCGGCGCCCG GCGCAGCCCG GGCCGCGGCT TGGGGCTTCG GGGGCGGCCG 
AGGCTCGGCT TCCACCCGGG GCCGCCGCCG CCACCGCCGC CGCCGCTGCT GTTGCTGTTT 
CTGCTCCTGC TGCCGCCGCC GCCGTTGCTG GCCGGCGCCA CGGCCGCCGC CGCCTCGCGG 
GAGCCCGACA GCCCATGCCG GCTCAAGACC GTCACCGTAT CCACGCTGCC CGCTCTGCGG 
GAAAGCGACA TCGGCTGGAG CGGTGCCCGC ACCGGGGCCG CGGCCGGGGC TGGGGCCGGG 
ACCGGAGCCG GGGCCGGAGC TGCTGCTGCT GCCGCCTCCG CCGCGTCCCC GGGCTCCGCG 
GGTTCAGCCG GCACAGCCGC GGAGTCTCGT CTCCTTCTCT TTGTGCGTAA TGAGCTCCCC 
GGGCGCATCG CGGTACAAGA TGACCTGGAC AACACTGAGC TGCCCTTCTT CACCCTGGAG 
ATGTCTGGCA CAGCAGCGGA CATTTCTTTG GTTCACTGGA GGCAGCAGTG GCTGGAGAAT 
GGCACATTGT ACTTCCACGT TTCCATGAGC AGCTCTGGGC AACTGGCTCA GGCCACTGCT 
CCCACACTCC AGGAGCCCTC GGAGATCGTT GAGGAACAGA TGCATATCCT CCACATTTCT 
GTGATGGGTG GACTCATTGC ACTTCTCCTC CTGCTGTTGG TGTTCACAGT GGCACTGTAT 
GCCCAGCGAC GCTGGCAGAA GCGCCGGCGC ATCCCACAGA AGAGCGCAAG TACAGAAGCT 
ACTCATGAGA TTCACTACAT CCCATCAGTG CTGCTGGGTC CTCAGGCCAG GGAAAGCTTC 
CGATCCTCCA GACTTCAGAC ACACAACTCA GTCATTGGTG TGCCTATCCG GGAAACCCCC 
ATCCTGGATG ACTATGACTA TGAAGAGGAG GAGGAACCAC CCAGGCGAGC CAACCACGTC 
TCCCGTGAGG ATGAGTTTGG TAGCCAGATG ACCCATGCCC TGGACAGCTT GGGAAGGCCA 
GGAGAAGAGA AAGTGGAATT TGAGAAGAAA GCAGCAGCTG AGGCGACACA GGAAACAGTG 
GAGTCCCTGA TGCAGAAGTT CAAGGAGAGT TTCCGTGCTA ACACACCAGT GGAGATCGGT 
CAGTTGCAGC CAGCCTCGCG CAGCAGCACC TCTGCAGGGA AGAGGAAGCG GAGGAACAAA 
TCTCGAGGGG GAATCAGCTT TGGGAGAACC AAGGGGACAT CAGGCTCAGA AGCAGATGAC 
GAAACACAGC TGACCTTCTA CACAGAGCAG TACCGCAGTC GCCGCCGCAG CAAAGGTTTA 
CTGAAAAGCC CTGTGAATAA GACAGCCTTA ACACTGATTG CTGTGAGCTC CTGCATCTTG 
GCCATGGTGT GTGGCAACCA GATGTCCTGT CCACTTACTG TGAAGGTGAC TTTGCATGTG 
CCTGAACACT TCATTGCAGA TGGGAGCAGC TTTGTGGTGA GTGAAGGAAG CTACCTGGAT 
ATCTCCGATT GGCTAAACCC TGCCAAGCTG TCTCTGTATT ACCAGATCAA TGCCACCTCG 
CCATGGGTGA GAGACCTCTG TGGACAGAGG ACGACAGATG CCTGTGAACA GCTCTGTGAC 
CCAGATACTG GGGAGTGCAG TTGTCATGAA GGTTACGCCC CTGACCCAGT CCACAGACAT 
CTATGTGTTC GCAGTGACTG GGGTCAAAGT GAAGGACCTT GGCCTTACAC AACACTGGAG 
AGGGGCTATG ATCTGGTGAC AGGAGAGCAA GCCCCTGAAA AGATTCTCAG GTCTACTTTC 
AGCTTGGGCC AAGGCCTCTG GCTTCCGGTC AGCAAAAGCT TTGTGGTGCC ACCGGTGGAG 
CTGTCCATCA ACCCCCTGGC CAGCTGCAAG ACGGATGTGC TCGTCACAGA AGACCCTGCG 
GATGTCAGGG AAGAAGCAAT GTTGTCCACG TACTTTGAAA CCATCAACGA CCTGCTGTCC 
TCCTTTGGTC CAGTCCGTGA CTGCTCAAGG AACAACGGGG GCTGTACCCG TAACTTCAAG 
TGTGTGTCTG ACCGCCAGGT GGACTCCTCG GGATGTGTGT GTCCTGAGGA ACTGAAGCCC 
ATGAAGGATG GCTCTGGCTG CTATGATCAC TCCAAGGGCA TCGACTGCTC TGATGGCTTT 
AATGGAGGGT GTGAGCAGCT TTGCCTACAA CAAACACTGC CTTTGCCCTA TGATACCACC 
TCCAGCACCA TCTTCATGTT CTGCGGCTGT GTGGAAGAGT ACAAGCTGGC TCCTGATGGG 
AAATCCTGTC TAATGCTCTC AGATGTCTGT GAGGGCCCCA AGTGCCTCAA ACCTGACTCC 
AAATTCAATG ACACCCTCTT TGGAGAGATG CTACATGGTT ACAACAACCG GACACAACAT 
GTAAACCAAG GCCAAGTCTT CCAAATGACA TTCAGGGAGA ATAACTTCAT CAAGGACTTT 
CCACAGCTGG CAGATGGGCT GTTGGTGATC CCGCTGCCAG TGGAAGAGCA ATGCCGGGGG 
GTTCTCTCTG AGCCCCTTCC AGACCTTCAG CTTCTCACAG GAGACATCAG GTATGATGAG 
GCCATGGGGT ACCCCATGGT GCAACAGTGG CGTGTCCGGA GCAATCTCTA TCGTGTGAAG 
CTGAGTACTA TCACCCTCTC AGCAGGCTTC ACCAATGTCC TAAAGATCCT GACCAAAGAG 
AGCAGTCGGG ATGAGCTGCT GTCCTTCATC CAGCACTATG GCTCCCACTA CATTGCCGAG 
GCTTTATACG GTTCTGAGCT CACCTGCATC ATCCATTTTC CCAGCAAGAA GGTCCAGCAG 
CAGCTATGGC TCCAATATCA GAAAGGTGTA AAGGCCCAAG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Intramembrane Proteolysis of Astrotactins.
The Journal of biological chemistry292(8)3506-3516(2017 02)
Chang H,Smallwood PM,Williams J,Nathans J

A dual-strategy expression screen for candidate connectivity labels in the developing thalamus.
PloS one12(5)e0177977(2017)
Bibollet-Bahena O,Okafuji T,Hokamp K,Tear G,Mitchell KJ

Regulation of Tlx3 by Pax6 is required for the restricted expression of Chrn?3 in Cerebellar Granule Neuron progenitors during development.
Scientific reports630337(2016 Jul)
Divya TS,Lalitha S,Parvathy S,Subashini C,Sanalkumar R,Dhanesh SB,Rasheed VA,Divya MS,Tole S,James J

Identification of Astrotactin2 as a Genetic Modifier That Regulates the Global Orientation of Mammalian Hair Follicles.
PLoS genetics11(9)e1005532(2015)
Chang H,Cahill H,Smallwood PM,Wang Y,Nathans J

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Astn2 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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