Atp8b1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atp8b1
Entrez Gene ID	54670
Full Name	ATPase, class I, type 8B, member 1
Synonyms	AI451886,FIC1,Ic
General protein information	Preferred Names ATPase, class I, type 8B, member 1 Names phospholipid-transporting ATPase IC ATPase 8B1, p type ATPase B1, class I P4-ATPase flippase complex alpha subunit ATP8B1 aminophospholipid translocase familial intrahepatic cholestasis type I probable phospholipid-transporting ATPase IC
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18\|18 E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001001488.3	NP_001001488.2	phospholipid-transporting ATPase IC
XM_006526105.3	XP_006526168.1	phospholipid-transporting ATPase IC isoform X1
XM_006526105.4	XP_006526168.1	phospholipid-transporting ATPase IC isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Atp8b1	NP_001099610.1
Danio rerio (zebrafish)	atp8b1	XP_001920510.4
Xenopus tropicalis (tropical clawed frog)	atp8b1	NP_001025562.1
	ATP8B1	XP_002800987.1
Mus musculus (house mouse)	Atp8b1	NP_001001488.2
Homo sapiens (human)	ATP8B1	NP_005594.1
Pan troglodytes (chimpanzee)	ATP8B1	XP_001139654.3
Canis lupus familiaris (dog)	ATP8B1	XP_533394.4
Bos taurus (cattle)	ATP8B1	NP_001179768.1
Gallus gallus (chicken)	ATP8B1	NP_001264704.1

Gene Ontology
Bibliography

Related articles in PubMed

The phospholipid flippase ATP8B1 mediates apical localization of the cystic fibrosis transmembrane regulator.
van der Mark VA, de Jonge HR, Chang JC, Ho-Mok KS, Duijst S, Vidovi? D, Carlon MS, Oude Elferink RP, Paulusma CC
Biochimica et biophysica acta1863(9)2280-8(2016 09)

ATP8B1-mediated spatial organization of Cdc42 signaling maintains singularity during enterocyte polarization.
Bruurs LJ, Donker L, Zwakenberg S, Zwartkruis FJ, Begthel H, Knisely AS, Posthuma G, van de Graaf SF, Paulusma CC, Bos JL
The Journal of cell biology210(7)1055-63(2015 Sep)

Resistance to noise-induced hearing loss in 129S6 and MOLF mice: identification of independent, overlapping, and interacting chromosomal regions.
Street VA, Kujawa SG, Manichaikul A, Broman KW, Kallman JC, Shilling DJ, Iwata AJ, Robinson LC, Robbins CA, Li J, Liberman MC, Tempel BL
Journal of the Association for Research in Otolaryngology : JARO15(5)721-38(2014 Oct)

Hepatic cytochrome P450 deficiency in mouse models for intrahepatic cholestasis predispose to bile salt-induced cholestasis.
Kunne C, de Graaff M, Duijst S, de Waart DR, Oude Elferink RP, Paulusma CC
Laboratory investigation; a journal of technical methods and pathology94(10)1103-13(2014 Oct)

miR-33 controls the expression of biliary transporters, and mediates statin- and diet-induced hepatotoxicity.
Allen RM, Marquart TJ, Albert CJ, Suchy FJ, Wang DQ, Ananthanarayanan M, Ford DA, Bald?n A
EMBO molecular medicine4(9)882-95(2012 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ATP8B1 is important for proper CFTR expression and function.
Title: The phospholipid flippase ATP8B1 mediates apical localization of the cystic fibrosis transmembrane regulator.

to become apically clustered, Cdc42 requires the interaction between its polybasic region and negatively charged membrane lipids provided by ATP8B1.
Title: ATP8B1-mediated spatial organization of Cdc42 signaling maintains singularity during enterocyte polarization.

The authors show that two of these transporters, ABCB11 and ATP8B1, are functional targets of miR-33, a micro-RNA that is expressed from within an intron of SREBP-2.
Title: miR-33 controls the expression of biliary transporters, and mediates statin- and diet-induced hepatotoxicity.

Flippase ATP8B1 and the floppase ABCB4 have complementary functions in maintaining canalicular membrane integrity.
Title: Complementary functions of the flippase ATP8B1 and the floppase ABCB4 in maintaining canalicular membrane integrity.

results unveil a new paradigm whereby Atp8b1 is a cardiolipin importer whose capacity to remove cardiolipin from lung fluid is exceeded during inflammation or when Atp8b1 is defective
Title: Dynamic regulation of cardiolipin by the lipid pump Atp8b1 determines the severity of lung injury in experimental pneumonia.

The following Atp8b1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atp8b1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu54705	XM_006526105.4 Latest version!	Mus musculus ATPase, class I, type 8B, member 1 (Atp8b1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu10371	NM_001001488.3 Latest version!	Mus musculus ATPase, class I, type 8B, member 1 (Atp8b1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$734.30 $1049.00
View Old Accession Versions >>
OMu54705	XM_006526105.3	Mus musculus ATPase, class I, type 8B, member 1 (Atp8b1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atp8b1 ( XM_006526105.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526168.1 Mus musculus ATPase, class I, type 8B, member 1 (Atp8b1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu54705
Clone ID Related Accession (Same CDS sequence)	XM_006526105.3 , XM_006526105.4
Accession Version	XM_006526105.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	phospholipid-transporting ATPase IC isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526105.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACAG AAAGAGACTC GGAAACAACA TTTGATGAGG AGTCTCAGCC CAACGATGAA 
GTGGTCCCCT ACAGTGATGA TGAAACTGAA GACGAACTTG AAGATCAGGG GTCCACGGTT 
GAACCAGAAC AGAATCGTGT CAACAGAGAA GCTGAGAAGA AGAGAGAGAC ATTCCGGAAA 
GATTGCACGT GGCAAGTCAA AGCAAATGAC CGAAAGTTCC ACGAACAACC TCATTTCATG 
AACACCAAGT TCTTTTGTAT TAAGGAGAGC AAATATGCGA GTAATGCAAT TAAAACATAC 
AAGTACAATG GATTTACCTT TCTACCCATG AATTTGTTTG AGCAGTTTAA GAGGGCAGCC 
AACTTCTATT TCCTCATCCT CCTGATCCTG CAGGCAATTC CTCAAATCTC CACCCTGGCA 
TGGTATACCA CACTTGTACC TCTGCTTTTG GTGCTGGGCA TCACGGCCAT CAAAGACCTG 
GTAGATGATG TGGCTCGTCA TAAAATGGAC AAGGAAATCA ACAATAGGAC GTGTGAAGTC 
ATTAAGGATG GCAGGTTCAA AATCATCAAG TGGAAAGATA TCCAGGTTGG AGATGTCATC 
CGCCTGAAGA AGAATGATTT TATTCCAGCT GACATCCTGC TGTTGTCCAG TTCAGAGCCT 
AACAGCCTCT GCTATGTAGA AACGGCTGAG CTCGACGGAG AAACCAATTT GAAGTTCAAA 
ATGGCCCTGG AAATCACGGA CCAATATCTC CAAATAGAAG ATAACTTGGC AACATTTGAT 
GGTTTCATCG AATGTGAAGA ACCAAACAAT CGGTTAGACA AGTTCACAGG AACGCTGTTT 
TGGAAGAACC AAAGCTTTCC TTTGGATGCT GATAAAATCC TGTTACGAGG TTGTGTGATA 
AGGAACACCG ATGTCTGCCA TGGCCTGGTC ATTTTTGCAG GTGCTGACAC AAAGATAATG 
AAGAATAGTG GGAAAACCAG ATTTAAAAGA ACCAAAATTG ATTACTTGAT GAACTACATG 
GTGTACACGA TCTTTATTGT TCTCATCCTG GTTTCTGCCG GCCTCGCCAT TGGCCACGCC 
TATTGGGAAG CCCAAGTTGG CAATTATTCC TGGTACCTCT ATGATGGAGA AAATGCCACA 
CCGTCCTACC GGGGATTCCT GAATTTCTGG GGCTACATCA TTGTATTGAA CACCATGGTG 
CCCATTTCTC TCTATGTCAG TGTGGAAGTG ATCCGTCTGG GACAGAGTCA TTTCATCAAC 
TGGGACCTGC AGATGTACTA TGCTGAGAAG GACACTCCTG CCAAGGCGAG GACCACCACC 
TTGAATGAGC AGCTAGGGCA GATTCATTAC ATCTTCTCTG ATAAGACGGG GACACTCACG 
CAAAATATCA TGACCTTCAA AAAGTGCTGC ATTAACGGGA CTATCTATGG AGACCATCGA 
GATGCTTCTC AACACAGCCA CAGCAAAATC GAGCTAGTGG ACTTTAGCTG GAATACATTT 
GCCGATGGAA AGCTTGCATT TTATGACCAT TACCTTATCG AGCAAATACA GTCAGGGAAA 
GAGCCAGAAG TACGACAGTT CTTCTTTTTG CTTTCGATTT GCCATACGGT CATGGTGGAC 
AGAATTGATG GTCAGATCAA CTACCAGGCG GCTTCTCCTG ATGAAGGCGC CCTGGTAAAC 
GCCGCCAGGA ACTTCGGCTT TGCCTTTCTC GCCCGGACGC AGAACACCAT CACCGTCAGC 
GAACTGGGCT CAGAGAGGAC CTACAACGTC CTTGCCATTT TGGATTTCAA CAGCGACCGA 
AAGCGGATGT CTATCATCGT GAGGACCCCA GAAGGTAGTA TCCGGCTGTA CTGTAAAGGT 
GCTGACACCG TGATTTATGA ACGGCTCCAT CGGATGAATC CTACGAAGCA AGAAACACAG 
GATGCCCTGG ATATCTTTGC AAGTGAGACT CTCAGGACCC TGTGCCTGTG CTACAAGGAA 
ATTGAAGAAA AAGAATTTAC AGAATGGAAT AATAAGTTTA TGGCCGCCAG CGTGGCCTCA 
AGCAACCGAG ACGAAGCGTT GGATAAAGTG TACGAGGAGA TTGAGAAAGA CTTGATTCTC 
TTGGGAGCTA CTGCTATTGA AGACAAGCTA CAGGATGGAG TCCCAGAAAC CATTTCAAAA 
CTTGCAAAAG CTGACATTAA GATCTGGGTA CTCACCGGAG ACAAAAAGGA AACTGCAGAA 
AACATAGGTT TTGCCTGTGA ACTCTTGACG GAAGACACTA CTATCTGCTA CGGAGAGGAC 
ATAAACTCTC TTCTTCACAC CAGGATGGAG AATCAGAGAA ACAGAGGTGG AGTCTCTGCG 
AAATTTGCAC CTCCTGTGTA TGAACCCTTT TTTCCACCTG GAGAAAACCG TGCCTTAATC 
ATCACTGGCT CCTGGCTGAA TGAAATCCTT CTAGAGAAGA AGACCAAGAG AAGTAAGATC 
CTGAAGCTGA AGTTCCCAAG GACAGAGGAG GAGAGGCGGA TGCGGAGCCA GAGCCGGAGA 
CGGCTGGAAG AGAAGAAAGA GCAGCGGCAA AAGAACTTCG TGGACCTGGC CTGCGAGTGC 
AGCGCCGTCA TCTGCTGCCG CGTCACGCCC AAGCAGAAGG CCATGGTGGT GGACCTGGTG 
AAGAGGTACA AGAAAGCCAT CACCCTGGCC ATCGGAGACG GGGCCAATGA TGTCAACATG 
ATCAAAAAGC TCCTCTCCTT TCCTCCTTGG CATGTGCTAA GAGTCTCCCC GCCCTGTCTT 
CTTCAGCAGG AGCCGTCTGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526168.1
CDS	1182..3965
Translation	MSTERDSETTFDEESQPNDEVVPYSDDETEDELEDQGSTVEPEQNRVNREAEKKRETFRKDCTWQVKANDRKFHEQPHFMNTKFFCIKESKYASNAIKTYKYNGFTFLPMNLFEQFKRAANFYFLILLILQAIPQISTLAWYTTLVPLLLVLGITAIKDLVDDVARHKMDKEINNRTCEVIKDGRFKIIKWKDIQVGDVIRLKKNDFIPADILLLSSSEPNSLCYVETAELDGETNLKFKMALEITDQYLQIEDNLATFDGFIECEEPNNRLDKFTGTLFWKNQSFPLDADKILLRGCVIRNTDVCHGLVIFAGADTKIMKNSGKTRFKRTKIDYLMNYMVYTIFIVLILVSAGLAIGHAYWEAQVGNYSWYLYDGENATPSYRGFLNFWGYIIVLNTMVPISLYVSVEVIRLGQSHFINWDLQMYYAEKDTPAKARTTTLNEQLGQIHYIFSDKTGTLTQNIMTFKKCCINGTIYGDHRDASQHSHSKIELVDFSWNTFADGKLAFYDHYLIEQIQSGKEPEVRQFFFLLSICHTVMVDRIDGQINYQAASPDEGALVNAARNFGFAFLARTQNTITVSELGSERTYNVLAILDFNSDRKRMSIIVRTPEGSIRLYCKGADTVIYERLHRMNPTKQETQDALDIFASETLRTLCLCYKEIEEKEFTEWNNKFMAASVASSNRDEALDKVYEEIEKDLILLGATAIEDKLQDGVPETISKLAKADIKIWVLTGDKKETAENIGFACELLTEDTTICYGEDINSLLHTRMENQRNRGGVSAKFAPPVYEPFFPPGENRALIITGSWLNEILLEKKTKRSKILKLKFPRTEEERRMRSQSRRRLEEKKEQRQKNFVDLACECSAVICCRVTPKQKAMVVDLVKRYKKAITLAIGDGANDVNMIKKLLSFPPWHVLRVSPPCLLQQEPSV

Target ORF information:

RefSeq Version	XM_006526105.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ATPase, class I, type 8B, member 1 (Atp8b1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526105.3

ORF Insert Sequence:

ATGAGCACAG AAAGAGACTC GGAAACAACA TTTGATGAGG AGTCTCAGCC CAACGATGAA 
GTGGTCCCCT ACAGTGATGA TGAAACTGAA GACGAACTTG AAGATCAGGG GTCCACGGTT 
GAACCAGAAC AGAATCGTGT CAACAGAGAA GCTGAGAAGA AGAGAGAGAC ATTCCGGAAA 
GATTGCACGT GGCAAGTCAA AGCAAATGAC CGAAAGTTCC ACGAACAACC TCATTTCATG 
AACACCAAGT TCTTTTGTAT TAAGGAGAGC AAATATGCGA GTAATGCAAT TAAAACATAC 
AAGTACAATG GATTTACCTT TCTACCCATG AATTTGTTTG AGCAGTTTAA GAGGGCAGCC 
AACTTCTATT TCCTCATCCT CCTGATCCTG CAGGCAATTC CTCAAATCTC CACCCTGGCA 
TGGTATACCA CACTTGTACC TCTGCTTTTG GTGCTGGGCA TCACGGCCAT CAAAGACCTG 
GTAGATGATG TGGCTCGTCA TAAAATGGAC AAGGAAATCA ACAATAGGAC GTGTGAAGTC 
ATTAAGGATG GCAGGTTCAA AATCATCAAG TGGAAAGATA TCCAGGTTGG AGATGTCATC 
CGCCTGAAGA AGAATGATTT TATTCCAGCT GACATCCTGC TGTTGTCCAG TTCAGAGCCT 
AACAGCCTCT GCTATGTAGA AACGGCTGAG CTCGACGGAG AAACCAATTT GAAGTTCAAA 
ATGGCCCTGG AAATCACGGA CCAATATCTC CAAATAGAAG ATAACTTGGC AACATTTGAT 
GGTTTCATCG AATGTGAAGA ACCAAACAAT CGGTTAGACA AGTTCACAGG AACGCTGTTT 
TGGAAGAACC AAAGCTTTCC TTTGGATGCT GATAAAATCC TGTTACGAGG TTGTGTGATA 
AGGAACACCG ATGTCTGCCA TGGCCTGGTC ATTTTTGCAG GTGCTGACAC AAAGATAATG 
AAGAATAGTG GGAAAACCAG ATTTAAAAGA ACCAAAATTG ATTACTTGAT GAACTACATG 
GTGTACACGA TCTTTATTGT TCTCATCCTG GTTTCTGCCG GCCTCGCCAT TGGCCACGCC 
TATTGGGAAG CCCAAGTTGG CAATTATTCC TGGTACCTCT ATGATGGAGA AAATGCCACA 
CCGTCCTACC GGGGATTCCT GAATTTCTGG GGCTACATCA TTGTATTGAA CACCATGGTG 
CCCATTTCTC TCTATGTCAG TGTGGAAGTG ATCCGTCTGG GACAGAGTCA TTTCATCAAC 
TGGGACCTGC AGATGTACTA TGCTGAGAAG GACACTCCTG CCAAGGCGAG GACCACCACC 
TTGAATGAGC AGCTAGGGCA GATTCATTAC ATCTTCTCTG ATAAGACGGG GACACTCACG 
CAAAATATCA TGACCTTCAA AAAGTGCTGC ATTAACGGGA CTATCTATGG AGACCATCGA 
GATGCTTCTC AACACAGCCA CAGCAAAATC GAGCTAGTGG ACTTTAGCTG GAATACATTT 
GCCGATGGAA AGCTTGCATT TTATGACCAT TACCTTATCG AGCAAATACA GTCAGGGAAA 
GAGCCAGAAG TACGACAGTT CTTCTTTTTG CTTTCGATTT GCCATACGGT CATGGTGGAC 
AGAATTGATG GTCAGATCAA CTACCAGGCG GCTTCTCCTG ATGAAGGCGC CCTGGTAAAC 
GCCGCCAGGA ACTTCGGCTT TGCCTTTCTC GCCCGGACGC AGAACACCAT CACCGTCAGC 
GAACTGGGCT CAGAGAGGAC CTACAACGTC CTTGCCATTT TGGATTTCAA CAGCGACCGA 
AAGCGGATGT CTATCATCGT GAGGACCCCA GAAGGTAGTA TCCGGCTGTA CTGTAAAGGT 
GCTGACACCG TGATTTATGA ACGGCTCCAT CGGATGAATC CTACGAAGCA AGAAACACAG 
GATGCCCTGG ATATCTTTGC AAGTGAGACT CTCAGGACCC TGTGCCTGTG CTACAAGGAA 
ATTGAAGAAA AAGAATTTAC AGAATGGAAT AATAAGTTTA TGGCCGCCAG CGTGGCCTCA 
AGCAACCGAG ACGAAGCGTT GGATAAAGTG TACGAGGAGA TTGAGAAAGA CTTGATTCTC 
TTGGGAGCTA CTGCTATTGA AGACAAGCTA CAGGATGGAG TCCCAGAAAC CATTTCAAAA 
CTTGCAAAAG CTGACATTAA GATCTGGGTA CTCACCGGAG ACAAAAAGGA AACTGCAGAA 
AACATAGGTT TTGCCTGTGA ACTCTTGACG GAAGACACTA CTATCTGCTA CGGAGAGGAC 
ATAAACTCTC TTCTTCACAC CAGGATGGAG AATCAGAGAA ACAGAGGTGG AGTCTCTGCG 
AAATTTGCAC CTCCTGTGTA TGAACCCTTT TTTCCACCTG GAGAAAACCG TGCCTTAATC 
ATCACTGGCT CCTGGCTGAA TGAAATCCTT CTAGAGAAGA AGACCAAGAG AAGTAAGATC 
CTGAAGCTGA AGTTCCCAAG GACAGAGGAG GAGAGGCGGA TGCGGAGCCA GAGCCGGAGA 
CGGCTGGAAG AGAAGAAAGA GCAGCGGCAA AAGAACTTCG TGGACCTGGC CTGCGAGTGC 
AGCGCCGTCA TCTGCTGCCG CGTCACGCCC AAGCAGAAGG CCATGGTGGT GGACCTGGTG 
AAGAGGTACA AGAAAGCCAT CACCCTGGCC ATCGGAGACG GGGCCAATGA TGTCAACATG 
ATCAAAAAGC TCCTCTCCTT TCCTCCTTGG CATGTGCTAA GAGTCTCCCC GCCCTGTCTT 
CTTCAGCAGG AGCCGTCTGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atp8b1 ( XM_006526105.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526168.1 Mus musculus ATPase, class I, type 8B, member 1 (Atp8b1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu54705
Clone ID Related Accession (Same CDS sequence)	XM_006526105.3 , XM_006526105.4
Accession Version	XM_006526105.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	phospholipid-transporting ATPase IC isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526105.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACAG AAAGAGACTC GGAAACAACA TTTGATGAGG AGTCTCAGCC CAACGATGAA 
GTGGTCCCCT ACAGTGATGA TGAAACTGAA GACGAACTTG AAGATCAGGG GTCCACGGTT 
GAACCAGAAC AGAATCGTGT CAACAGAGAA GCTGAGAAGA AGAGAGAGAC ATTCCGGAAA 
GATTGCACGT GGCAAGTCAA AGCAAATGAC CGAAAGTTCC ACGAACAACC TCATTTCATG 
AACACCAAGT TCTTTTGTAT TAAGGAGAGC AAATATGCGA GTAATGCAAT TAAAACATAC 
AAGTACAATG GATTTACCTT TCTACCCATG AATTTGTTTG AGCAGTTTAA GAGGGCAGCC 
AACTTCTATT TCCTCATCCT CCTGATCCTG CAGGCAATTC CTCAAATCTC CACCCTGGCA 
TGGTATACCA CACTTGTACC TCTGCTTTTG GTGCTGGGCA TCACGGCCAT CAAAGACCTG 
GTAGATGATG TGGCTCGTCA TAAAATGGAC AAGGAAATCA ACAATAGGAC GTGTGAAGTC 
ATTAAGGATG GCAGGTTCAA AATCATCAAG TGGAAAGATA TCCAGGTTGG AGATGTCATC 
CGCCTGAAGA AGAATGATTT TATTCCAGCT GACATCCTGC TGTTGTCCAG TTCAGAGCCT 
AACAGCCTCT GCTATGTAGA AACGGCTGAG CTCGACGGAG AAACCAATTT GAAGTTCAAA 
ATGGCCCTGG AAATCACGGA CCAATATCTC CAAATAGAAG ATAACTTGGC AACATTTGAT 
GGTTTCATCG AATGTGAAGA ACCAAACAAT CGGTTAGACA AGTTCACAGG AACGCTGTTT 
TGGAAGAACC AAAGCTTTCC TTTGGATGCT GATAAAATCC TGTTACGAGG TTGTGTGATA 
AGGAACACCG ATGTCTGCCA TGGCCTGGTC ATTTTTGCAG GTGCTGACAC AAAGATAATG 
AAGAATAGTG GGAAAACCAG ATTTAAAAGA ACCAAAATTG ATTACTTGAT GAACTACATG 
GTGTACACGA TCTTTATTGT TCTCATCCTG GTTTCTGCCG GCCTCGCCAT TGGCCACGCC 
TATTGGGAAG CCCAAGTTGG CAATTATTCC TGGTACCTCT ATGATGGAGA AAATGCCACA 
CCGTCCTACC GGGGATTCCT GAATTTCTGG GGCTACATCA TTGTATTGAA CACCATGGTG 
CCCATTTCTC TCTATGTCAG TGTGGAAGTG ATCCGTCTGG GACAGAGTCA TTTCATCAAC 
TGGGACCTGC AGATGTACTA TGCTGAGAAG GACACTCCTG CCAAGGCGAG GACCACCACC 
TTGAATGAGC AGCTAGGGCA GATTCATTAC ATCTTCTCTG ATAAGACGGG GACACTCACG 
CAAAATATCA TGACCTTCAA AAAGTGCTGC ATTAACGGGA CTATCTATGG AGACCATCGA 
GATGCTTCTC AACACAGCCA CAGCAAAATC GAGCTAGTGG ACTTTAGCTG GAATACATTT 
GCCGATGGAA AGCTTGCATT TTATGACCAT TACCTTATCG AGCAAATACA GTCAGGGAAA 
GAGCCAGAAG TACGACAGTT CTTCTTTTTG CTTTCGATTT GCCATACGGT CATGGTGGAC 
AGAATTGATG GTCAGATCAA CTACCAGGCG GCTTCTCCTG ATGAAGGCGC CCTGGTAAAC 
GCCGCCAGGA ACTTCGGCTT TGCCTTTCTC GCCCGGACGC AGAACACCAT CACCGTCAGC 
GAACTGGGCT CAGAGAGGAC CTACAACGTC CTTGCCATTT TGGATTTCAA CAGCGACCGA 
AAGCGGATGT CTATCATCGT GAGGACCCCA GAAGGTAGTA TCCGGCTGTA CTGTAAAGGT 
GCTGACACCG TGATTTATGA ACGGCTCCAT CGGATGAATC CTACGAAGCA AGAAACACAG 
GATGCCCTGG ATATCTTTGC AAGTGAGACT CTCAGGACCC TGTGCCTGTG CTACAAGGAA 
ATTGAAGAAA AAGAATTTAC AGAATGGAAT AATAAGTTTA TGGCCGCCAG CGTGGCCTCA 
AGCAACCGAG ACGAAGCGTT GGATAAAGTG TACGAGGAGA TTGAGAAAGA CTTGATTCTC 
TTGGGAGCTA CTGCTATTGA AGACAAGCTA CAGGATGGAG TCCCAGAAAC CATTTCAAAA 
CTTGCAAAAG CTGACATTAA GATCTGGGTA CTCACCGGAG ACAAAAAGGA AACTGCAGAA 
AACATAGGTT TTGCCTGTGA ACTCTTGACG GAAGACACTA CTATCTGCTA CGGAGAGGAC 
ATAAACTCTC TTCTTCACAC CAGGATGGAG AATCAGAGAA ACAGAGGTGG AGTCTCTGCG 
AAATTTGCAC CTCCTGTGTA TGAACCCTTT TTTCCACCTG GAGAAAACCG TGCCTTAATC 
ATCACTGGCT CCTGGCTGAA TGAAATCCTT CTAGAGAAGA AGACCAAGAG AAGTAAGATC 
CTGAAGCTGA AGTTCCCAAG GACAGAGGAG GAGAGGCGGA TGCGGAGCCA GAGCCGGAGA 
CGGCTGGAAG AGAAGAAAGA GCAGCGGCAA AAGAACTTCG TGGACCTGGC CTGCGAGTGC 
AGCGCCGTCA TCTGCTGCCG CGTCACGCCC AAGCAGAAGG CCATGGTGGT GGACCTGGTG 
AAGAGGTACA AGAAAGCCAT CACCCTGGCC ATCGGAGACG GGGCCAATGA TGTCAACATG 
ATCAAAAAGC TCCTCTCCTT TCCTCCTTGG CATGTGCTAA GAGTCTCCCC GCCCTGTCTT 
CTTCAGCAGG AGCCGTCTGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526168.1
CDS	596..3379
Translation	MSTERDSETTFDEESQPNDEVVPYSDDETEDELEDQGSTVEPEQNRVNREAEKKRETFRKDCTWQVKANDRKFHEQPHFMNTKFFCIKESKYASNAIKTYKYNGFTFLPMNLFEQFKRAANFYFLILLILQAIPQISTLAWYTTLVPLLLVLGITAIKDLVDDVARHKMDKEINNRTCEVIKDGRFKIIKWKDIQVGDVIRLKKNDFIPADILLLSSSEPNSLCYVETAELDGETNLKFKMALEITDQYLQIEDNLATFDGFIECEEPNNRLDKFTGTLFWKNQSFPLDADKILLRGCVIRNTDVCHGLVIFAGADTKIMKNSGKTRFKRTKIDYLMNYMVYTIFIVLILVSAGLAIGHAYWEAQVGNYSWYLYDGENATPSYRGFLNFWGYIIVLNTMVPISLYVSVEVIRLGQSHFINWDLQMYYAEKDTPAKARTTTLNEQLGQIHYIFSDKTGTLTQNIMTFKKCCINGTIYGDHRDASQHSHSKIELVDFSWNTFADGKLAFYDHYLIEQIQSGKEPEVRQFFFLLSICHTVMVDRIDGQINYQAASPDEGALVNAARNFGFAFLARTQNTITVSELGSERTYNVLAILDFNSDRKRMSIIVRTPEGSIRLYCKGADTVIYERLHRMNPTKQETQDALDIFASETLRTLCLCYKEIEEKEFTEWNNKFMAASVASSNRDEALDKVYEEIEKDLILLGATAIEDKLQDGVPETISKLAKADIKIWVLTGDKKETAENIGFACELLTEDTTICYGEDINSLLHTRMENQRNRGGVSAKFAPPVYEPFFPPGENRALIITGSWLNEILLEKKTKRSKILKLKFPRTEEERRMRSQSRRRLEEKKEQRQKNFVDLACECSAVICCRVTPKQKAMVVDLVKRYKKAITLAIGDGANDVNMIKKLLSFPPWHVLRVSPPCLLQQEPSV

Target ORF information:

RefSeq Version	XM_006526105.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ATPase, class I, type 8B, member 1 (Atp8b1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526105.4

ORF Insert Sequence:

ATGAGCACAG AAAGAGACTC GGAAACAACA TTTGATGAGG AGTCTCAGCC CAACGATGAA 
GTGGTCCCCT ACAGTGATGA TGAAACTGAA GACGAACTTG AAGATCAGGG GTCCACGGTT 
GAACCAGAAC AGAATCGTGT CAACAGAGAA GCTGAGAAGA AGAGAGAGAC ATTCCGGAAA 
GATTGCACGT GGCAAGTCAA AGCAAATGAC CGAAAGTTCC ACGAACAACC TCATTTCATG 
AACACCAAGT TCTTTTGTAT TAAGGAGAGC AAATATGCGA GTAATGCAAT TAAAACATAC 
AAGTACAATG GATTTACCTT TCTACCCATG AATTTGTTTG AGCAGTTTAA GAGGGCAGCC 
AACTTCTATT TCCTCATCCT CCTGATCCTG CAGGCAATTC CTCAAATCTC CACCCTGGCA 
TGGTATACCA CACTTGTACC TCTGCTTTTG GTGCTGGGCA TCACGGCCAT CAAAGACCTG 
GTAGATGATG TGGCTCGTCA TAAAATGGAC AAGGAAATCA ACAATAGGAC GTGTGAAGTC 
ATTAAGGATG GCAGGTTCAA AATCATCAAG TGGAAAGATA TCCAGGTTGG AGATGTCATC 
CGCCTGAAGA AGAATGATTT TATTCCAGCT GACATCCTGC TGTTGTCCAG TTCAGAGCCT 
AACAGCCTCT GCTATGTAGA AACGGCTGAG CTCGACGGAG AAACCAATTT GAAGTTCAAA 
ATGGCCCTGG AAATCACGGA CCAATATCTC CAAATAGAAG ATAACTTGGC AACATTTGAT 
GGTTTCATCG AATGTGAAGA ACCAAACAAT CGGTTAGACA AGTTCACAGG AACGCTGTTT 
TGGAAGAACC AAAGCTTTCC TTTGGATGCT GATAAAATCC TGTTACGAGG TTGTGTGATA 
AGGAACACCG ATGTCTGCCA TGGCCTGGTC ATTTTTGCAG GTGCTGACAC AAAGATAATG 
AAGAATAGTG GGAAAACCAG ATTTAAAAGA ACCAAAATTG ATTACTTGAT GAACTACATG 
GTGTACACGA TCTTTATTGT TCTCATCCTG GTTTCTGCCG GCCTCGCCAT TGGCCACGCC 
TATTGGGAAG CCCAAGTTGG CAATTATTCC TGGTACCTCT ATGATGGAGA AAATGCCACA 
CCGTCCTACC GGGGATTCCT GAATTTCTGG GGCTACATCA TTGTATTGAA CACCATGGTG 
CCCATTTCTC TCTATGTCAG TGTGGAAGTG ATCCGTCTGG GACAGAGTCA TTTCATCAAC 
TGGGACCTGC AGATGTACTA TGCTGAGAAG GACACTCCTG CCAAGGCGAG GACCACCACC 
TTGAATGAGC AGCTAGGGCA GATTCATTAC ATCTTCTCTG ATAAGACGGG GACACTCACG 
CAAAATATCA TGACCTTCAA AAAGTGCTGC ATTAACGGGA CTATCTATGG AGACCATCGA 
GATGCTTCTC AACACAGCCA CAGCAAAATC GAGCTAGTGG ACTTTAGCTG GAATACATTT 
GCCGATGGAA AGCTTGCATT TTATGACCAT TACCTTATCG AGCAAATACA GTCAGGGAAA 
GAGCCAGAAG TACGACAGTT CTTCTTTTTG CTTTCGATTT GCCATACGGT CATGGTGGAC 
AGAATTGATG GTCAGATCAA CTACCAGGCG GCTTCTCCTG ATGAAGGCGC CCTGGTAAAC 
GCCGCCAGGA ACTTCGGCTT TGCCTTTCTC GCCCGGACGC AGAACACCAT CACCGTCAGC 
GAACTGGGCT CAGAGAGGAC CTACAACGTC CTTGCCATTT TGGATTTCAA CAGCGACCGA 
AAGCGGATGT CTATCATCGT GAGGACCCCA GAAGGTAGTA TCCGGCTGTA CTGTAAAGGT 
GCTGACACCG TGATTTATGA ACGGCTCCAT CGGATGAATC CTACGAAGCA AGAAACACAG 
GATGCCCTGG ATATCTTTGC AAGTGAGACT CTCAGGACCC TGTGCCTGTG CTACAAGGAA 
ATTGAAGAAA AAGAATTTAC AGAATGGAAT AATAAGTTTA TGGCCGCCAG CGTGGCCTCA 
AGCAACCGAG ACGAAGCGTT GGATAAAGTG TACGAGGAGA TTGAGAAAGA CTTGATTCTC 
TTGGGAGCTA CTGCTATTGA AGACAAGCTA CAGGATGGAG TCCCAGAAAC CATTTCAAAA 
CTTGCAAAAG CTGACATTAA GATCTGGGTA CTCACCGGAG ACAAAAAGGA AACTGCAGAA 
AACATAGGTT TTGCCTGTGA ACTCTTGACG GAAGACACTA CTATCTGCTA CGGAGAGGAC 
ATAAACTCTC TTCTTCACAC CAGGATGGAG AATCAGAGAA ACAGAGGTGG AGTCTCTGCG 
AAATTTGCAC CTCCTGTGTA TGAACCCTTT TTTCCACCTG GAGAAAACCG TGCCTTAATC 
ATCACTGGCT CCTGGCTGAA TGAAATCCTT CTAGAGAAGA AGACCAAGAG AAGTAAGATC 
CTGAAGCTGA AGTTCCCAAG GACAGAGGAG GAGAGGCGGA TGCGGAGCCA GAGCCGGAGA 
CGGCTGGAAG AGAAGAAAGA GCAGCGGCAA AAGAACTTCG TGGACCTGGC CTGCGAGTGC 
AGCGCCGTCA TCTGCTGCCG CGTCACGCCC AAGCAGAAGG CCATGGTGGT GGACCTGGTG 
AAGAGGTACA AGAAAGCCAT CACCCTGGCC ATCGGAGACG GGGCCAATGA TGTCAACATG 
ATCAAAAAGC TCCTCTCCTT TCCTCCTTGG CATGTGCTAA GAGTCTCCCC GCCCTGTCTT 
CTTCAGCAGG AGCCGTCTGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atp8b1 ( NM_001001488.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001001488.2 Mus musculus ATPase, class I, type 8B, member 1 (Atp8b1), mRNA.

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CloneID	OMu10371
Clone ID Related Accession (Same CDS sequence)	NM_001001488.3
Accession Version	NM_001001488.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-01
Organism	Mus musculus(house mouse)
Product	phospholipid-transporting ATPase IC
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC137585.3, AK036959.1, AC102268.11, BQ714379.1 and AA242626.1. On May 9, 2006 this sequence version replaced NM_001001488.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK157316.1, AY506548.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGAGCACAG AAAGAGACTC GGAAACAACA TTTGATGAGG AGTCTCAGCC CAACGATGAA 
GTGGTCCCCT ACAGTGATGA TGAAACTGAA GACGAACTTG AAGATCAGGG GTCCACGGTT 
GAACCAGAAC AGAATCGTGT CAACAGAGAA GCTGAGAAGA AGAGAGAGAC ATTCCGGAAA 
GATTGCACGT GGCAAGTCAA AGCAAATGAC CGAAAGTTCC ACGAACAACC TCATTTCATG 
AACACCAAGT TCTTTTGTAT TAAGGAGAGC AAATATGCGA GTAATGCAAT TAAAACATAC 
AAGTACAATG GATTTACCTT TCTACCCATG AATTTGTTTG AGCAGTTTAA GAGGGCAGCC 
AACTTCTATT TCCTCATCCT CCTGATCCTG CAGGCAATTC CTCAAATCTC CACCCTGGCA 
TGGTATACCA CACTTGTACC TCTGCTTTTG GTGCTGGGCA TCACGGCCAT CAAAGACCTG 
GTAGATGATG TGGCTCGTCA TAAAATGGAC AAGGAAATCA ACAATAGGAC GTGTGAAGTC 
ATTAAGGATG GCAGGTTCAA AATCATCAAG TGGAAAGATA TCCAGGTTGG AGATGTCATC 
CGCCTGAAGA AGAATGATTT TATTCCAGCT GACATCCTGC TGTTGTCCAG TTCAGAGCCT 
AACAGCCTCT GCTATGTAGA AACGGCTGAG CTCGACGGAG AAACCAATTT GAAGTTCAAA 
ATGGCCCTGG AAATCACGGA CCAATATCTC CAAATAGAAG ATAACTTGGC AACATTTGAT 
GGTTTCATCG AATGTGAAGA ACCAAACAAT CGGTTAGACA AGTTCACAGG AACGCTGTTT 
TGGAAGAACC AAAGCTTTCC TTTGGATGCT GATAAAATCC TGTTACGAGG TTGTGTGATA 
AGGAACACCG ATGTCTGCCA TGGCCTGGTC ATTTTTGCAG GTGCTGACAC AAAGATAATG 
AAGAATAGTG GGAAAACCAG ATTTAAAAGA ACCAAAATTG ATTACTTGAT GAACTACATG 
GTGTACACGA TCTTTATTGT TCTCATCCTG GTTTCTGCCG GCCTCGCCAT TGGCCACGCC 
TATTGGGAAG CCCAAGTTGG CAATTATTCC TGGTACCTCT ATGATGGAGA AAATGCCACA 
CCGTCCTACC GGGGATTCCT GAATTTCTGG GGCTACATCA TTGTATTGAA CACCATGGTG 
CCCATTTCTC TCTATGTCAG TGTGGAAGTG ATCCGTCTGG GACAGAGTCA TTTCATCAAC 
TGGGACCTGC AGATGTACTA TGCTGAGAAG GACACTCCTG CCAAGGCGAG GACCACCACC 
TTGAATGAGC AGCTAGGGCA GATTCATTAC ATCTTCTCTG ATAAGACGGG GACACTCACG 
CAAAATATCA TGACCTTCAA AAAGTGCTGC ATTAACGGGA CTATCTATGG AGACCATCGA 
GATGCTTCTC AACACAGCCA CAGCAAAATC GAGCTAGTGG ACTTTAGCTG GAATACATTT 
GCCGATGGAA AGCTTGCATT TTATGACCAT TACCTTATCG AGCAAATACA GTCAGGGAAA 
GAGCCAGAAG TACGACAGTT CTTCTTTTTG CTTTCGATTT GCCATACGGT CATGGTGGAC 
AGAATTGATG GTCAGATCAA CTACCAGGCG GCTTCTCCTG ATGAAGGCGC CCTGGTAAAC 
GCCGCCAGGA ACTTCGGCTT TGCCTTTCTC GCCCGGACGC AGAACACCAT CACCGTCAGC 
GAACTGGGCT CAGAGAGGAC CTACAACGTC CTTGCCATTT TGGATTTCAA CAGCGACCGA 
AAGCGGATGT CTATCATCGT GAGGACCCCA GAAGGTAGTA TCCGGCTGTA CTGTAAAGGT 
GCTGACACCG TGATTTATGA ACGGCTCCAT CGGATGAATC CTACGAAGCA AGAAACACAG 
GATGCCCTGG ATATCTTTGC AAGTGAGACT CTCAGGACCC TGTGCCTGTG CTACAAGGAA 
ATTGAAGAAA AAGAATTTAC AGAATGGAAT AATAAGTTTA TGGCCGCCAG CGTGGCCTCA 
AGCAACCGAG ACGAAGCGTT GGATAAAGTG TACGAGGAGA TTGAGAAAGA CTTGATTCTC 
TTGGGAGCTA CTGCTATTGA AGACAAGCTA CAGGATGGAG TCCCAGAAAC CATTTCAAAA 
CTTGCAAAAG CTGACATTAA GATCTGGGTA CTCACCGGAG ACAAAAAGGA AACTGCAGAA 
AACATAGGTT TTGCCTGTGA ACTCTTGACG GAAGACACTA CTATCTGCTA CGGAGAGGAC 
ATAAACTCTC TTCTTCACAC CAGGATGGAG AATCAGAGAA ACAGAGGTGG AGTCTCTGCG 
AAATTTGCAC CTCCTGTGTA TGAACCCTTT TTTCCACCTG GAGAAAACCG TGCCTTAATC 
ATCACTGGCT CCTGGCTGAA TGAAATCCTT CTAGAGAAGA AGACCAAGAG AAGTAAGATC 
CTGAAGCTGA AGTTCCCAAG GACAGAGGAG GAGAGGCGGA TGCGGAGCCA GAGCCGGAGA 
CGGCTGGAAG AGAAGAAAGA GCAGCGGCAA AAGAACTTCG TGGACCTGGC CTGCGAGTGC 
AGCGCCGTCA TCTGCTGCCG CGTCACGCCC AAGCAGAAGG CCATGGTGGT GGACCTGGTG 
AAGAGGTACA AGAAAGCCAT CACCCTGGCC ATCGGAGACG GGGCCAATGA TGTCAACATG 
ATCAAAACTG CCCACATTGG TGTTGGAATA AGTGGACAAG AAGGGATGCA AGCTGTCATG 
TCCAGTGACT ATTCTTTTGC TCAGTTCAGA TATCTGCAGC GGTTGCTGCT GGTCCACGGC 
CGGTGGTCTT ACATAAGGAT GTGCAAGTTC CTACGATACT TCTTTTACAA GAACTTTGCC 
TTTACTTTAG TTCATTTCTG GTACTCCTTC TTCAATGGCT ACTCAGCCCA GACGGCGTAT 
GAGGATTGGT TTATCACCCT CTACAACGTG CTGTACTCCA GTTTGCCTGT GCTCCTCATG 
GGGCTGCTGG ATCAGGACGT GAGTGACAAG CTGAGCCTCC GATTCCCTGG GCTGTATGTA 
GTGGGGCAGA GGGACTTGCT GTTCAACTAT AAGAGATTCT TTGTCAGTTT GCTGCATGGG 
GTCTTAACCT CAATGGTCCT CTTCTTCATA CCGCTTGGTG CTTATCTGCA AACAGTGGGA 
CAGGATGGGG AGGCGCCTTC AGACTACCAG TCCTTCGCCG TCACGGTGGC CTCCGCTCTA 
GTGATAACGG TCAACTTCCA GATTGGCTTG GATACTTCAT ACTGGACTTT TGTGAATGCC 
TTTTCAATTT TCGGCAGCAT CGCACTGTAC TTCGGCATCA TGTTTGATTT CCACAGTGCT 
GGGATACACG TTCTCTTTCC GTCCGCATTT CAGTTCACAG GTACAGCTTC CAATGCCCTG 
AGACAGCCAT ACATCTGGCT GACCATCATC CTGACGGTTG CCGTGTGCTT ACTACCTGTC 
GTCGCCATCC GTTTCCTGTC CATGACCATT TGGCCCTCAG AAAGTGATAA GATCCAGAAG 
CATCGAAAGC GATTGAAGGC TGAAGAGCAG TGGAAACGGC GGCAGAGCGT GTTCCGGAGG 
GGAGTATCTT CCAGGCGCTC AGCCTATGCC TTTTCGCATC AGAGAGGCTA CGCAGACCTC 
ATCTCCTCTG GCCGCAGCAT CCGCAAGAAG CGCTCGCCCC TGGATGCCAT CATCGCGGAT 
GGCACGGCTG AGTACCGGCG CACAGTGGAG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001488.2
CDS	324..4079
Translation	MSTERDSETTFDEESQPNDEVVPYSDDETEDELEDQGSTVEPEQNRVNREAEKKRETFRKDCTWQVKANDRKFHEQPHFMNTKFFCIKESKYASNAIKTYKYNGFTFLPMNLFEQFKRAANFYFLILLILQAIPQISTLAWYTTLVPLLLVLGITAIKDLVDDVARHKMDKEINNRTCEVIKDGRFKIIKWKDIQVGDVIRLKKNDFIPADILLLSSSEPNSLCYVETAELDGETNLKFKMALEITDQYLQIEDNLATFDGFIECEEPNNRLDKFTGTLFWKNQSFPLDADKILLRGCVIRNTDVCHGLVIFAGADTKIMKNSGKTRFKRTKIDYLMNYMVYTIFIVLILVSAGLAIGHAYWEAQVGNYSWYLYDGENATPSYRGFLNFWGYIIVLNTMVPISLYVSVEVIRLGQSHFINWDLQMYYAEKDTPAKARTTTLNEQLGQIHYIFSDKTGTLTQNIMTFKKCCINGTIYGDHRDASQHSHSKIELVDFSWNTFADGKLAFYDHYLIEQIQSGKEPEVRQFFFLLSICHTVMVDRIDGQINYQAASPDEGALVNAARNFGFAFLARTQNTITVSELGSERTYNVLAILDFNSDRKRMSIIVRTPEGSIRLYCKGADTVIYERLHRMNPTKQETQDALDIFASETLRTLCLCYKEIEEKEFTEWNNKFMAASVASSNRDEALDKVYEEIEKDLILLGATAIEDKLQDGVPETISKLAKADIKIWVLTGDKKETAENIGFACELLTEDTTICYGEDINSLLHTRMENQRNRGGVSAKFAPPVYEPFFPPGENRALIITGSWLNEILLEKKTKRSKILKLKFPRTEEERRMRSQSRRRLEEKKEQRQKNFVDLACECSAVICCRVTPKQKAMVVDLVKRYKKAITLAIGDGANDVNMIKTAHIGVGISGQEGMQAVMSSDYSFAQFRYLQRLLLVHGRWSYIRMCKFLRYFFYKNFAFTLVHFWYSFFNGYSAQTAYEDWFITLYNVLYSSLPVLLMGLLDQDVSDKLSLRFPGLYVVGQRDLLFNYKRFFVSLLHGVLTSMVLFFIPLGAYLQTVGQDGEAPSDYQSFAVTVASALVITVNFQIGLDTSYWTFVNAFSIFGSIALYFGIMFDFHSAGIHVLFPSAFQFTGTASNALRQPYIWLTIILTVAVCLLPVVAIRFLSMTIWPSESDKIQKHRKRLKAEEQWKRRQSVFRRGVSSRRSAYAFSHQRGYADLISSGRSIRKKRSPLDAIIADGTAEYRRTVES

Target ORF information:

RefSeq Version	NM_001001488.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ATPase, class I, type 8B, member 1 (Atp8b1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001488.3

ORF Insert Sequence:

ATGAGCACAG AAAGAGACTC GGAAACAACA TTTGATGAGG AGTCTCAGCC CAACGATGAA 
GTGGTCCCCT ACAGTGATGA TGAAACTGAA GACGAACTTG AAGATCAGGG GTCCACGGTT 
GAACCAGAAC AGAATCGTGT CAACAGAGAA GCTGAGAAGA AGAGAGAGAC ATTCCGGAAA 
GATTGCACGT GGCAAGTCAA AGCAAATGAC CGAAAGTTCC ACGAACAACC TCATTTCATG 
AACACCAAGT TCTTTTGTAT TAAGGAGAGC AAATATGCGA GTAATGCAAT TAAAACATAC 
AAGTACAATG GATTTACCTT TCTACCCATG AATTTGTTTG AGCAGTTTAA GAGGGCAGCC 
AACTTCTATT TCCTCATCCT CCTGATCCTG CAGGCAATTC CTCAAATCTC CACCCTGGCA 
TGGTATACCA CACTTGTACC TCTGCTTTTG GTGCTGGGCA TCACGGCCAT CAAAGACCTG 
GTAGATGATG TGGCTCGTCA TAAAATGGAC AAGGAAATCA ACAATAGGAC GTGTGAAGTC 
ATTAAGGATG GCAGGTTCAA AATCATCAAG TGGAAAGATA TCCAGGTTGG AGATGTCATC 
CGCCTGAAGA AGAATGATTT TATTCCAGCT GACATCCTGC TGTTGTCCAG TTCAGAGCCT 
AACAGCCTCT GCTATGTAGA AACGGCTGAG CTCGACGGAG AAACCAATTT GAAGTTCAAA 
ATGGCCCTGG AAATCACGGA CCAATATCTC CAAATAGAAG ATAACTTGGC AACATTTGAT 
GGTTTCATCG AATGTGAAGA ACCAAACAAT CGGTTAGACA AGTTCACAGG AACGCTGTTT 
TGGAAGAACC AAAGCTTTCC TTTGGATGCT GATAAAATCC TGTTACGAGG TTGTGTGATA 
AGGAACACCG ATGTCTGCCA TGGCCTGGTC ATTTTTGCAG GTGCTGACAC AAAGATAATG 
AAGAATAGTG GGAAAACCAG ATTTAAAAGA ACCAAAATTG ATTACTTGAT GAACTACATG 
GTGTACACGA TCTTTATTGT TCTCATCCTG GTTTCTGCCG GCCTCGCCAT TGGCCACGCC 
TATTGGGAAG CCCAAGTTGG CAATTATTCC TGGTACCTCT ATGATGGAGA AAATGCCACA 
CCGTCCTACC GGGGATTCCT GAATTTCTGG GGCTACATCA TTGTATTGAA CACCATGGTG 
CCCATTTCTC TCTATGTCAG TGTGGAAGTG ATCCGTCTGG GACAGAGTCA TTTCATCAAC 
TGGGACCTGC AGATGTACTA TGCTGAGAAG GACACTCCTG CCAAGGCGAG GACCACCACC 
TTGAATGAGC AGCTAGGGCA GATTCATTAC ATCTTCTCTG ATAAGACGGG GACACTCACG 
CAAAATATCA TGACCTTCAA AAAGTGCTGC ATTAACGGGA CTATCTATGG AGACCATCGA 
GATGCTTCTC AACACAGCCA CAGCAAAATC GAGCTAGTGG ACTTTAGCTG GAATACATTT 
GCCGATGGAA AGCTTGCATT TTATGACCAT TACCTTATCG AGCAAATACA GTCAGGGAAA 
GAGCCAGAAG TACGACAGTT CTTCTTTTTG CTTTCGATTT GCCATACGGT CATGGTGGAC 
AGAATTGATG GTCAGATCAA CTACCAGGCG GCTTCTCCTG ATGAAGGCGC CCTGGTAAAC 
GCCGCCAGGA ACTTCGGCTT TGCCTTTCTC GCCCGGACGC AGAACACCAT CACCGTCAGC 
GAACTGGGCT CAGAGAGGAC CTACAACGTC CTTGCCATTT TGGATTTCAA CAGCGACCGA 
AAGCGGATGT CTATCATCGT GAGGACCCCA GAAGGTAGTA TCCGGCTGTA CTGTAAAGGT 
GCTGACACCG TGATTTATGA ACGGCTCCAT CGGATGAATC CTACGAAGCA AGAAACACAG 
GATGCCCTGG ATATCTTTGC AAGTGAGACT CTCAGGACCC TGTGCCTGTG CTACAAGGAA 
ATTGAAGAAA AAGAATTTAC AGAATGGAAT AATAAGTTTA TGGCCGCCAG CGTGGCCTCA 
AGCAACCGAG ACGAAGCGTT GGATAAAGTG TACGAGGAGA TTGAGAAAGA CTTGATTCTC 
TTGGGAGCTA CTGCTATTGA AGACAAGCTA CAGGATGGAG TCCCAGAAAC CATTTCAAAA 
CTTGCAAAAG CTGACATTAA GATCTGGGTA CTCACCGGAG ACAAAAAGGA AACTGCAGAA 
AACATAGGTT TTGCCTGTGA ACTCTTGACG GAAGACACTA CTATCTGCTA CGGAGAGGAC 
ATAAACTCTC TTCTTCACAC CAGGATGGAG AATCAGAGAA ACAGAGGTGG AGTCTCTGCG 
AAATTTGCAC CTCCTGTGTA TGAACCCTTT TTTCCACCTG GAGAAAACCG TGCCTTAATC 
ATCACTGGCT CCTGGCTGAA TGAAATCCTT CTAGAGAAGA AGACCAAGAG AAGTAAGATC 
CTGAAGCTGA AGTTCCCAAG GACAGAGGAG GAGAGGCGGA TGCGGAGCCA GAGCCGGAGA 
CGGCTGGAAG AGAAGAAAGA GCAGCGGCAA AAGAACTTCG TGGACCTGGC CTGCGAGTGC 
AGCGCCGTCA TCTGCTGCCG CGTCACGCCC AAGCAGAAGG CCATGGTGGT GGACCTGGTG 
AAGAGGTACA AGAAAGCCAT CACCCTGGCC ATCGGAGACG GGGCCAATGA TGTCAACATG 
ATCAAAACTG CCCACATTGG TGTTGGAATA AGTGGACAAG AAGGGATGCA AGCTGTCATG 
TCCAGTGACT ATTCTTTTGC TCAGTTCAGA TATCTGCAGC GGTTGCTGCT GGTCCACGGC 
CGGTGGTCTT ACATAAGGAT GTGCAAGTTC CTACGATACT TCTTTTACAA GAACTTTGCC 
TTTACTTTAG TTCATTTCTG GTACTCCTTC TTCAATGGCT ACTCAGCCCA GACGGCGTAT 
GAGGATTGGT TTATCACCCT CTACAACGTG CTGTACTCCA GTTTGCCTGT GCTCCTCATG 
GGGCTGCTGG ATCAGGACGT GAGTGACAAG CTGAGCCTCC GATTCCCTGG GCTGTATGTA 
GTGGGGCAGA GGGACTTGCT GTTCAACTAT AAGAGATTCT TTGTCAGTTT GCTGCATGGG 
GTCTTAACCT CAATGGTCCT CTTCTTCATA CCGCTTGGTG CTTATCTGCA AACAGTGGGA 
CAGGATGGGG AGGCGCCTTC AGACTACCAG TCCTTCGCCG TCACGGTGGC CTCCGCTCTA 
GTGATAACGG TCAACTTCCA GATTGGCTTG GATACTTCAT ACTGGACTTT TGTGAATGCC 
TTTTCAATTT TCGGCAGCAT CGCACTGTAC TTCGGCATCA TGTTTGATTT CCACAGTGCT 
GGGATACACG TTCTCTTTCC GTCCGCATTT CAGTTCACAG GTACAGCTTC CAATGCCCTG 
AGACAGCCAT ACATCTGGCT GACCATCATC CTGACGGTTG CCGTGTGCTT ACTACCTGTC 
GTCGCCATCC GTTTCCTGTC CATGACCATT TGGCCCTCAG AAAGTGATAA GATCCAGAAG 
CATCGAAAGC GATTGAAGGC TGAAGAGCAG TGGAAACGGC GGCAGAGCGT GTTCCGGAGG 
GGAGTATCTT CCAGGCGCTC AGCCTATGCC TTTTCGCATC AGAGAGGCTA CGCAGACCTC 
ATCTCCTCTG GCCGCAGCAT CCGCAAGAAG CGCTCGCCCC TGGATGCCAT CATCGCGGAT 
GGCACGGCTG AGTACCGGCG CACAGTGGAG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The phospholipid flippase ATP8B1 mediates apical localization of the cystic fibrosis transmembrane regulator.
Biochimica et biophysica acta1863(9)2280-8(2016 09)
van der Mark VA,de Jonge HR,Chang JC,Ho-Mok KS,Duijst S,Vidovi? D,Carlon MS,Oude Elferink RP,Paulusma CC

ATP8B1-mediated spatial organization of Cdc42 signaling maintains singularity during enterocyte polarization.
The Journal of cell biology210(7)1055-63(2015 Sep)
Bruurs LJ,Donker L,Zwakenberg S,Zwartkruis FJ,Begthel H,Knisely AS,Posthuma G,van de Graaf SF,Paulusma CC,Bos JL

Resistance to noise-induced hearing loss in 129S6 and MOLF mice: identification of independent, overlapping, and interacting chromosomal regions.
Journal of the Association for Research in Otolaryngology : JARO15(5)721-38(2014 Oct)
Street VA,Kujawa SG,Manichaikul A,Broman KW,Kallman JC,Shilling DJ,Iwata AJ,Robinson LC,Robbins CA,Li J,Liberman MC,Tempel BL

Hepatic cytochrome P450 deficiency in mouse models for intrahepatic cholestasis predispose to bile salt-induced cholestasis.
Laboratory investigation; a journal of technical methods and pathology94(10)1103-13(2014 Oct)
Kunne C,de Graaff M,Duijst S,de Waart DR,Oude Elferink RP,Paulusma CC

miR-33 controls the expression of biliary transporters, and mediates statin- and diet-induced hepatotoxicity.
EMBO molecular medicine4(9)882-95(2012 Sep)
Allen RM,Marquart TJ,Albert CJ,Suchy FJ,Wang DQ,Ananthanarayanan M,Ford DA,Bald?n A

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Atp8b1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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