Spag11b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spag11b
Entrez Gene ID	546038
Full Name	sperm associated antigen 11B
Synonyms	EG546038,Ep2c/h,Spag11c/h
General protein information	Preferred Names sperm associated antigen 11B Names sperm associated antigen 11B beta-defensin-like peptide sperm-associated antigen 11c/h
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 A1.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001034905.2	NP_001030077.1	sperm associated antigen 11B isoform 3 precursor
NM_001039563.3	NP_001034652.2	sperm associated antigen 11B isoform c precursor
NM_001286493.1	NP_001273422.1	sperm associated antigen 11B isoform 1 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SPAG11B	NP_001103707.1
	SPAG11B	NP_001103726.1
Homo sapiens (human)	SPAG11B	NP_478110.1
Rattus norvegicus (Norway rat)	LOC100362580	XP_006253439.1
Canis lupus familiaris (dog)	SPAG11B	NP_001165015.1
Mus musculus (house mouse)	Spag11b	NP_001273422.1

Gene Ontology
Bibliography

Related articles in PubMed

Manual annotation and analysis of the defensin gene cluster in the C57BL/6J mouse reference genome.
Amid C, Rehaume LM, Brown KL, Gilbert JG, Dougan G, Hancock RE, Harrow JL
BMC genomics10606(2009 Dec)

Cross-species analysis of the mammalian beta-defensin gene family: presence of syntenic gene clusters and preferential expression in the male reproductive tract.
Patil AA, Cai Y, Sang Y, Blecha F, Zhang G
Physiological genomics23(1)5-17(2005 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Spag11b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spag11b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu00730	NM_001039563.3 Latest version!	Mus musculus sperm associated antigen 11B (Spag11b), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu09341	NM_001286493.1 Latest version!	Mus musculus sperm associated antigen 11B (Spag11b), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu12792	NM_001034905.2 Latest version!	Mus musculus sperm associated antigen 11B (Spag11b), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spag11b ( NM_001039563.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001034652.2 Mus musculus sperm associated antigen 11B (Spag11b), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu00730
Clone ID Related Accession (Same CDS sequence)	NM_001039563.3
Accession Version	NM_001039563.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Mus musculus(house mouse)
Product	sperm associated antigen 11B isoform c precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK136627.1 and DQ012055.1. On Nov 7, 2013 this sequence version replaced NM_001039563.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform c, which is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC145549.1, AY552530.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849376, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGATACCAA GACTCCTTCC TTTCTTTGCC AGTCTACTTT TTGCAGCCCT GCTGTTTCCA 
GGCCTGTCCA ATGCCTCGTC CATTAACCAC TTAGTCACTG AACCTCCCAG TTTCCCCAAG 
GACGAATTTC CTGCTCGAGG AGTAAATGGG TCTCAGTTGT TACATCACAG AGTGAAACGA 
TTACCTCCAC GCACCCCCCC TTACCACGAG CCTGAACCAA ATTACCAAAT TGTCAACTGC 
AAGAAAAGTG AAGGACAATG TCAAGAATAC TGTAATTTCA TGGAAACACA AGTGGGCTAC 
TGTTCAAAAA AGAAAGAACC CTGCTGCTTA CATCCGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034652.2
CDS	40..381
Translation	MIPRLLPFFASLLFAALLFPGLSNASSINHLVTEPPSFPKDEFPARGVNGSQLLHHRVKRLPPRTPPYHEPEPNYQIVNCKKSEGQCQEYCNFMETQVGYCSKKKEPCCLHPF

Target ORF information:

RefSeq Version	NM_001039563.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 11B (Spag11b), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039563.3

ORF Insert Sequence:

ATGATACCAA GACTCCTTCC TTTCTTTGCC AGTCTACTTT TTGCAGCCCT GCTGTTTCCA 
GGCCTGTCCA ATGCCTCGTC CATTAACCAC TTAGTCACTG AACCTCCCAG TTTCCCCAAG 
GACGAATTTC CTGCTCGAGG AGTAAATGGG TCTCAGTTGT TACATCACAG AGTGAAACGA 
TTACCTCCAC GCACCCCCCC TTACCACGAG CCTGAACCAA ATTACCAAAT TGTCAACTGC 
AAGAAAAGTG AAGGACAATG TCAAGAATAC TGTAATTTCA TGGAAACACA AGTGGGCTAC 
TGTTCAAAAA AGAAAGAACC CTGCTGCTTA CATCCGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag11b ( NM_001286493.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001273422.1 Mus musculus sperm associated antigen 11B (Spag11b), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu09341
Clone ID Related Accession (Same CDS sequence)	NM_001286493.1
Accession Version	NM_001286493.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Mus musculus(house mouse)
Product	sperm associated antigen 11B isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK136627.1, DQ012055.1 and AC116394.22. Transcript Variant: This variant (1) encodes the longest protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC145547.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849376, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGATACCAA GACTCCTTCC TTTCTTTGCC AGTCTACTTT TTGCAGCCCT GCTGTTTCCA 
GCAGGCCTGT CCAATGCCTC GTCCATTAAC CACTTAGTCA CTGAACCTCC CAGTTTCCCC 
AAGGACGAAT TTCCTGCTCG AGGAGTAAAT GGGTCTCAGT TGTTACATCA CAGAGTGAAA 
CGATTACCTC CACGCACCCC CCCTTACCAC GAGCCTGAAC CAAATTACCA AATTGTCAAC 
TGCAAGAAAA GTGAAGGACA ATGTCAAGAA TACTGTAATT TCATGGAAAC ACAAGTGGGC 
TACTGTTCAA AAAAGAAAGA ACCCTGCTGC TTACATCCGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273422.1
CDS	40..384
Translation	MIPRLLPFFASLLFAALLFPAGLSNASSINHLVTEPPSFPKDEFPARGVNGSQLLHHRVKRLPPRTPPYHEPEPNYQIVNCKKSEGQCQEYCNFMETQVGYCSKKKEPCCLHPF

Target ORF information:

RefSeq Version	NM_001286493.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 11B (Spag11b), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286493.1

ORF Insert Sequence:

ATGATACCAA GACTCCTTCC TTTCTTTGCC AGTCTACTTT TTGCAGCCCT GCTGTTTCCA 
GCAGGCCTGT CCAATGCCTC GTCCATTAAC CACTTAGTCA CTGAACCTCC CAGTTTCCCC 
AAGGACGAAT TTCCTGCTCG AGGAGTAAAT GGGTCTCAGT TGTTACATCA CAGAGTGAAA 
CGATTACCTC CACGCACCCC CCCTTACCAC GAGCCTGAAC CAAATTACCA AATTGTCAAC 
TGCAAGAAAA GTGAAGGACA ATGTCAAGAA TACTGTAATT TCATGGAAAC ACAAGTGGGC 
TACTGTTCAA AAAAGAAAGA ACCCTGCTGC TTACATCCGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag11b ( NM_001034905.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001030077.1 Mus musculus sperm associated antigen 11B (Spag11b), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu12792
Clone ID Related Accession (Same CDS sequence)	NM_001034905.2
Accession Version	NM_001034905.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-19
Organism	Mus musculus(house mouse)
Product	sperm associated antigen 11B isoform 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK136627.1. On Apr 6, 2007 this sequence version replaced NM_001034905.1. Transcript Variant: This variant (3, also called h) includes a 3' terminal exon which extends past a splice site that is used in variant 1. It encodes isoform 3 (also called h) which is shorter and has a distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK136627.1, BY720985.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849376, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGATACCAA GACTCCTTCC TTTCTTTGCC AGTCTACTTT TTGCAGCCCT GCTGTTTCCA 
GCAGGCCTGT CCAATGCCTC GTCCATTAAC CACTTAGTCA CTGAACCTCC CAGTTTCCCC 
AAGGACGAAT TTCCTGCTCG AGGAGTAAAT GGGTCTCAGT TGTTACATCA CAGAGTGAAA 
CGATTACCTC CACGCACCCC CCCTTACCAC GGTAAGTCAA GCACCCAAGA TCAAGGCAAG 
TTCATCCACT TGGGAAATAC TGAGCGCATG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001030077.1
CDS	40..312
Translation	MIPRLLPFFASLLFAALLFPAGLSNASSINHLVTEPPSFPKDEFPARGVNGSQLLHHRVKRLPPRTPPYHGKSSTQDQGKFIHLGNTERM

Target ORF information:

RefSeq Version	NM_001034905.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 11B (Spag11b), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001034905.2

ORF Insert Sequence:

ATGATACCAA GACTCCTTCC TTTCTTTGCC AGTCTACTTT TTGCAGCCCT GCTGTTTCCA 
GCAGGCCTGT CCAATGCCTC GTCCATTAAC CACTTAGTCA CTGAACCTCC CAGTTTCCCC 
AAGGACGAAT TTCCTGCTCG AGGAGTAAAT GGGTCTCAGT TGTTACATCA CAGAGTGAAA 
CGATTACCTC CACGCACCCC CCCTTACCAC GGTAAGTCAA GCACCCAAGA TCAAGGCAAG 
TTCATCCACT TGGGAAATAC TGAGCGCATG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Manual annotation and analysis of the defensin gene cluster in the C57BL/6J mouse reference genome.
BMC genomics10606(2009 Dec)
Amid C,Rehaume LM,Brown KL,Gilbert JG,Dougan G,Hancock RE,Harrow JL

Cross-species analysis of the mammalian beta-defensin gene family: presence of syntenic gene clusters and preferential expression in the male reproductive tract.
Physiological genomics23(1)5-17(2005 Sep)
Patil AA,Cai Y,Sang Y,Blecha F,Zhang G