Pum3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pum3
Entrez Gene ID	52874
Full Name	pumilio RNA-binding family member 3
Synonyms	1110069H02Rik,AA675048,D19Bwg1357e
General protein information	Preferred Names pumilio RNA-binding family member 3 Names pumilio homolog 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19 C1\|19 21.96 cM
Summary	This gene encodes a member of the evolutionarily conserved Pumilio and Fem-3 mRNA-binding factor family of proteins, which are characterized by tandem 36 amino acid pumilio homolog domains and which function in diverse biological processes. This protein belongs to a group of atypical Pumilio and Fem-3 mRNA-binding factor proteins, whose members are distinguished from other Pumilio and Fem-3 mRNA-binding factor proteins by a novel protein fold with 11 pumilio homolog domains and an ability to bind to DNA and single- and double-stranded RNA without sequence specificity. In mouse, lower levels of gene expression have been correlated with increased testicular germ cell tumors. A pseudogene of this gene is found on chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006527183.3	XP_006527246.1	pumilio homolog 3 isoform X1
XM_006527183.4	XP_006527246.1	pumilio homolog 3 isoform X1
NM_177474.5	NP_803425.1	pumilio homolog 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	KIAA0020	NP_055693.4
Bos taurus (cattle)	KIAA0020	NP_001091499.1
Mus musculus (house mouse)	D19Bwg1357e	NP_803425.1
Danio rerio (zebrafish)	im:7148292	XP_003199363.1
Pan troglodytes (chimpanzee)	KIAA0020	XP_520461.3
Arabidopsis thaliana (thale cress)	PUM24	NP_566559.1
Oryza sativa (rice)	Os03g0831100	NP_001051791.1
Xenopus tropicalis (tropical clawed frog)	kiaa0020	NP_001116267.1
Canis lupus familiaris (dog)	KIAA0020	XP_533539.4
Gallus gallus (chicken)	KIAA0020	NP_001026608.1
	KIAA0020	XP_001084706.2
Caenorhabditis elegans (roundworm)	puf-12	NP_496178.1

Gene Ontology
Bibliography

Related articles in PubMed

A divergent Pumilio repeat protein family for pre-rRNA processing and mRNA localization.
Qiu C, McCann KL, Wine RN, Baserga SJ, Hall TM
Proceedings of the National Academy of Sciences of the United States of America111(52)18554-9(2014 Dec)

A comparative study of protein carbonylation and mitochondrial dysfunction using the neurotoxicants 1,3-dinitrobenzene, 3-nitropropionic acid, and 3-chloropropanediol.
Steiner SR, Milton E, Philbert MA
Neurotoxicology3774-84(2013 Jul)

A novel puf-A gene predicted from evolutionary analysis is involved in the development of eyes and primordial germ-cells.
Kuo MW, Wang SH, Chang JC, Chang CH, Huang LJ, Lin HH, Yu AL, Li WH, Yu J
PloS one4(3)e4980(2009)

Testicular germ cell tumor susceptibility genes from the consomic 129.MOLF-Chr19 mouse strain.
Zhu R, Ji Y, Xiao L, Matin A
Mammalian genome : official journal of the International Mammalian Genome Society18(8)584-95(2007 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pum3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pum3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu23715	NM_177474.5 Latest version!	Mus musculus pumilio RNA-binding family member 3 (Pum3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu23715	XM_006527183.4 Latest version!	Mus musculus pumilio RNA-binding family member 3 (Pum3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu23715	XM_006527183.3	Mus musculus pumilio RNA-binding family member 3 (Pum3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pum3 ( NM_177474.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_803425.1 Mus musculus pumilio RNA-binding family member 3 (Pum3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu23715
Clone ID Related Accession (Same CDS sequence)	XM_006527183.3 , XM_006527183.4 , NM_177474.5
Accession Version	NM_177474.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Mus musculus(house mouse)
Product	pumilio homolog 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC124973.8, BY251590.1, BY289042.1, AK141893.1 and BC041111.1. On May 21, 2015 this sequence version replaced NM_177474.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the protein. ##Evidence-Data-START## Transcript exon combination :: BC041111.1, SRR1660815.20969.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164143 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGAAG TCAAAGGGAA GAAAAAGTTT ACAGGAAAGA GTCCACAGAC ATCACAAGGA 
AAGAATAAAT TTCATAAGAA CAGTGAGTCT AGTTCTTCAA AGACATTCCC AAGGAAAGCT 
GTTAAGGAAG GCGGACCTAA AGTCACATCT AAGAACTTTG AGAAAGGTGC CACAAAACCT 
GGCAAGAAAG GTGTGAAGCA GTTCAAGAAC AAGCCACAAG GGGGCAAAGG ACCGAAGGAC 
AAATTTCAGA AGGCAAATAA ATTCAGCAAG AAGCGGAAAT TCCAGCCAGA TGGTGAAAGT 
GATGAATCAG GAGCCAAGAA ACCCAAGTGG GATGACTTCA AAAAGAAGAA GAAAGAGCTG 
AAGCAGAGCA GGCAGCTCAG TGACAAGACC AACTATGACA TCGTGGTTCG AGCAAAGCAC 
ATTTGGGAGA GCTTGAGAAG AAAAGATTGT GACAAGGAAA AACGGGTGAA GCTCATGAGT 
GATTTGCAGA AGTTGATTCA AGGGAAAATT AAAACTATCG CCTTTGCACA TGACTCGACG 
CGCGTGATCC AGTGCTTCAT TCAGTATGGG AACGAGGAGC AGAGGAAGCA GGCTTTCCAA 
GAGTTGCAAG GCGACTTGGT TGAATTAAGT AAAGCCAAAT ATTCCAGGAA TATCGTTAAG 
AAATTTCTCA TGTATGGAAG TAAGCCACAA GTTGCGGAGA TAATCAGAAG TTTTAAAGGT 
CATGTGAGGA AGATGCTGCG GCATTCGGAG GCATCGGCCA TTGTGGAGTA CGCATACAAC 
GACAAAGCCA TTTTGGAACA GAGGAACATG CTGACAGAGG AGCTCTATGG GAACACGTTT 
CAGCTTTACA AGTCAGCAGA TCACCCAACG CTGGACAAGG TGCTGGAGCT GCAGCCAGCA 
AAGCTAGAGC TTATCATGGA CGAGATGAAG CAGATTCTGA CACCGATGGC CCAAAAGGAA 
GCTGTGATTA AGCACTCACT CGTTCATAAA GTATTCTTGG ACTTTTTTAC CTACGCACCC 
CCAAAACCTC GATCAGAACT AATTGAAGCC ATCCGGGAAG CAGTGGTGTA CCTGGCCCAC 
ACACACGATG GCGCCAGAGT GGCCATGCAC TGCCTGTGGC ACGGCACACC CAAGGACAGG 
AAAGTGATCG TGAAAACGAT GAAGACGTAT GTGGAGAAGG TTGCCAATGG CCAGTACTCT 
CACTTGGTTC TCCTGGCAGC GTTTGACTGT ATTGATGATA CTAAGCTTGT GAAGCAGATA 
ATCATATCAG AAATCATCAG TTCCTTGCCC AGCATAGTTA ATGACAAATA CGGAAGGAAA 
GTCCTCTTGT ACTTGATGAG CCCCAGAGAC CCCGCCCACA CGGTGCCCGA GCTCATCGAG 
CTTCTGCAGA AGGGTGATGG CAACGCGCAC AGCAAGAAGG ACACTGCAAT CCGCCGGCGT 
GAGCTCTTGG AGTCCATCTC TCCAGCTTTG CTGAGCTACC TGCAAGGACA CACTCAAGAG 
GTGGTGCTGG ATAAGTCTGC ATGTGTGCTG GTGTCTGACA TGCTGGGATC TGCTACTGGA 
GATGTTCAGC CTGCTATGGA TGCCATCGCC AGTTTGGCAG CAGCAGAACT GCATCCTGGT 
GGCAAGGACG GAGAGCTTCA TGTTGCTGAG CATCCTGCAG GACATCTGGT TCTCAAATGG 
TTACTAGAGC AAGATAAAAA GATGAAAGAA AGCGGAAAGG AAGGTTGTTT TGCAAAAACC 
CTTGTAGAGC GTGTTGGTAT GAAGAACCTC AAGTCCTGGG CCAGCATCAA CCGAGGTGCC 
ATTATTCTTT CCAGCCTTCT TCAGAGTTGT GACCAAGAAG TTGTAAACAA AGTCAAAGGT 
GGACTGAAAC CCCTGATTCC TACCTTGGAA AAAAACAAAA GCAGCAGCAG AGGCATCCAG 
ACCCTGCTTG AGAAGCTGAC TGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_803425.1
CDS	125..2071
Translation	MMEVKGKKKFTGKSPQTSQGKNKFHKNSESSSSKTFPRKAVKEGGPKVTSKNFEKGATKPGKKGVKQFKNKPQGGKGPKDKFQKANKFSKKRKFQPDGESDESGAKKPKWDDFKKKKKELKQSRQLSDKTNYDIVVRAKHIWESLRRKDCDKEKRVKLMSDLQKLIQGKIKTIAFAHDSTRVIQCFIQYGNEEQRKQAFQELQGDLVELSKAKYSRNIVKKFLMYGSKPQVAEIIRSFKGHVRKMLRHSEASAIVEYAYNDKAILEQRNMLTEELYGNTFQLYKSADHPTLDKVLELQPAKLELIMDEMKQILTPMAQKEAVIKHSLVHKVFLDFFTYAPPKPRSELIEAIREAVVYLAHTHDGARVAMHCLWHGTPKDRKVIVKTMKTYVEKVANGQYSHLVLLAAFDCIDDTKLVKQIIISEIISSLPSIVNDKYGRKVLLYLMSPRDPAHTVPELIELLQKGDGNAHSKKDTAIRRRELLESISPALLSYLQGHTQEVVLDKSACVLVSDMLGSATGDVQPAMDAIASLAAAELHPGGKDGELHVAEHPAGHLVLKWLLEQDKKMKESGKEGCFAKTLVERVGMKNLKSWASINRGAIILSSLLQSCDQEVVNKVKGGLKPLIPTLEKNKSSSRGIQTLLEKLTA

Target ORF information:

RefSeq Version	NM_177474.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus pumilio RNA-binding family member 3 (Pum3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177474.5

ORF Insert Sequence:

ATGATGGAAG TCAAAGGGAA GAAAAAGTTT ACAGGAAAGA GTCCACAGAC ATCACAAGGA 
AAGAATAAAT TTCATAAGAA CAGTGAGTCT AGTTCTTCAA AGACATTCCC AAGGAAAGCT 
GTTAAGGAAG GCGGACCTAA AGTCACATCT AAGAACTTTG AGAAAGGTGC CACAAAACCT 
GGCAAGAAAG GTGTGAAGCA GTTCAAGAAC AAGCCACAAG GGGGCAAAGG ACCGAAGGAC 
AAATTTCAGA AGGCAAATAA ATTCAGCAAG AAGCGGAAAT TCCAGCCAGA TGGTGAAAGT 
GATGAATCAG GAGCCAAGAA ACCCAAGTGG GATGACTTCA AAAAGAAGAA GAAAGAGCTG 
AAGCAGAGCA GGCAGCTCAG TGACAAGACC AACTATGACA TCGTGGTTCG AGCAAAGCAC 
ATTTGGGAGA GCTTGAGAAG AAAAGATTGT GACAAGGAAA AACGGGTGAA GCTCATGAGT 
GATTTGCAGA AGTTGATTCA AGGGAAAATT AAAACTATCG CCTTTGCACA TGACTCGACG 
CGCGTGATCC AGTGCTTCAT TCAGTATGGG AACGAGGAGC AGAGGAAGCA GGCTTTCCAA 
GAGTTGCAAG GCGACTTGGT TGAATTAAGT AAAGCCAAAT ATTCCAGGAA TATCGTTAAG 
AAATTTCTCA TGTATGGAAG TAAGCCACAA GTTGCGGAGA TAATCAGAAG TTTTAAAGGT 
CATGTGAGGA AGATGCTGCG GCATTCGGAG GCATCGGCCA TTGTGGAGTA CGCATACAAC 
GACAAAGCCA TTTTGGAACA GAGGAACATG CTGACAGAGG AGCTCTATGG GAACACGTTT 
CAGCTTTACA AGTCAGCAGA TCACCCAACG CTGGACAAGG TGCTGGAGCT GCAGCCAGCA 
AAGCTAGAGC TTATCATGGA CGAGATGAAG CAGATTCTGA CACCGATGGC CCAAAAGGAA 
GCTGTGATTA AGCACTCACT CGTTCATAAA GTATTCTTGG ACTTTTTTAC CTACGCACCC 
CCAAAACCTC GATCAGAACT AATTGAAGCC ATCCGGGAAG CAGTGGTGTA CCTGGCCCAC 
ACACACGATG GCGCCAGAGT GGCCATGCAC TGCCTGTGGC ACGGCACACC CAAGGACAGG 
AAAGTGATCG TGAAAACGAT GAAGACGTAT GTGGAGAAGG TTGCCAATGG CCAGTACTCT 
CACTTGGTTC TCCTGGCAGC GTTTGACTGT ATTGATGATA CTAAGCTTGT GAAGCAGATA 
ATCATATCAG AAATCATCAG TTCCTTGCCC AGCATAGTTA ATGACAAATA CGGAAGGAAA 
GTCCTCTTGT ACTTGATGAG CCCCAGAGAC CCCGCCCACA CGGTGCCCGA GCTCATCGAG 
CTTCTGCAGA AGGGTGATGG CAACGCGCAC AGCAAGAAGG ACACTGCAAT CCGCCGGCGT 
GAGCTCTTGG AGTCCATCTC TCCAGCTTTG CTGAGCTACC TGCAAGGACA CACTCAAGAG 
GTGGTGCTGG ATAAGTCTGC ATGTGTGCTG GTGTCTGACA TGCTGGGATC TGCTACTGGA 
GATGTTCAGC CTGCTATGGA TGCCATCGCC AGTTTGGCAG CAGCAGAACT GCATCCTGGT 
GGCAAGGACG GAGAGCTTCA TGTTGCTGAG CATCCTGCAG GACATCTGGT TCTCAAATGG 
TTACTAGAGC AAGATAAAAA GATGAAAGAA AGCGGAAAGG AAGGTTGTTT TGCAAAAACC 
CTTGTAGAGC GTGTTGGTAT GAAGAACCTC AAGTCCTGGG CCAGCATCAA CCGAGGTGCC 
ATTATTCTTT CCAGCCTTCT TCAGAGTTGT GACCAAGAAG TTGTAAACAA AGTCAAAGGT 
GGACTGAAAC CCCTGATTCC TACCTTGGAA AAAAACAAAA GCAGCAGCAG AGGCATCCAG 
ACCCTGCTTG AGAAGCTGAC TGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pum3 ( XM_006527183.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527246.1 Mus musculus pumilio RNA-binding family member 3 (Pum3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu23715
Clone ID Related Accession (Same CDS sequence)	XM_006527183.3 , XM_006527183.4 , NM_177474.5
Accession Version	XM_006527183.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	pumilio homolog 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527183.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG TCAAAGGGAA GAAAAAGTTT ACAGGAAAGA GTCCACAGAC ATCACAAGGA 
AAGAATAAAT TTCATAAGAA CAGTGAGTCT AGTTCTTCAA AGACATTCCC AAGGAAAGCT 
GTTAAGGAAG GCGGACCTAA AGTCACATCT AAGAACTTTG AGAAAGGTGC CACAAAACCT 
GGCAAGAAAG GTGTGAAGCA GTTCAAGAAC AAGCCACAAG GGGGCAAAGG ACCGAAGGAC 
AAATTTCAGA AGGCAAATAA ATTCAGCAAG AAGCGGAAAT TCCAGCCAGA TGGTGAAAGT 
GATGAATCAG GAGCCAAGAA ACCCAAGTGG GATGACTTCA AAAAGAAGAA GAAAGAGCTG 
AAGCAGAGCA GGCAGCTCAG TGACAAGACC AACTATGACA TCGTGGTTCG AGCAAAGCAC 
ATTTGGGAGA GCTTGAGAAG AAAAGATTGT GACAAGGAAA AACGGGTGAA GCTCATGAGT 
GATTTGCAGA AGTTGATTCA AGGGAAAATT AAAACTATCG CCTTTGCACA TGACTCGACG 
CGCGTGATCC AGTGCTTCAT TCAGTATGGG AACGAGGAGC AGAGGAAGCA GGCTTTCCAA 
GAGTTGCAAG GCGACTTGGT TGAATTAAGT AAAGCCAAAT ATTCCAGGAA TATCGTTAAG 
AAATTTCTCA TGTATGGAAG TAAGCCACAA GTTGCGGAGA TAATCAGAAG TTTTAAAGGT 
CATGTGAGGA AGATGCTGCG GCATTCGGAG GCATCGGCCA TTGTGGAGTA CGCATACAAC 
GACAAAGCCA TTTTGGAACA GAGGAACATG CTGACAGAGG AGCTCTATGG GAACACGTTT 
CAGCTTTACA AGTCAGCAGA TCACCCAACG CTGGACAAGG TGCTGGAGCT GCAGCCAGCA 
AAGCTAGAGC TTATCATGGA CGAGATGAAG CAGATTCTGA CACCGATGGC CCAAAAGGAA 
GCTGTGATTA AGCACTCACT CGTTCATAAA GTATTCTTGG ACTTTTTTAC CTACGCACCC 
CCAAAACCTC GATCAGAACT AATTGAAGCC ATCCGGGAAG CAGTGGTGTA CCTGGCCCAC 
ACACACGATG GCGCCAGAGT GGCCATGCAC TGCCTGTGGC ACGGCACACC CAAGGACAGG 
AAAGTGATCG TGAAAACGAT GAAGACGTAT GTGGAGAAGG TTGCCAATGG CCAGTACTCT 
CACTTGGTTC TCCTGGCAGC GTTTGACTGT ATTGATGATA CTAAGCTTGT GAAGCAGATA 
ATCATATCAG AAATCATCAG TTCCTTGCCC AGCATAGTTA ATGACAAATA CGGAAGGAAA 
GTCCTCTTGT ACTTGATGAG CCCCAGAGAC CCCGCCCACA CGGTGCCCGA GCTCATCGAG 
CTTCTGCAGA AGGGTGATGG CAACGCGCAC AGCAAGAAGG ACACTGCAAT CCGCCGGCGT 
GAGCTCTTGG AGTCCATCTC TCCAGCTTTG CTGAGCTACC TGCAAGGACA CACTCAAGAG 
GTGGTGCTGG ATAAGTCTGC ATGTGTGCTG GTGTCTGACA TGCTGGGATC TGCTACTGGA 
GATGTTCAGC CTGCTATGGA TGCCATCGCC AGTTTGGCAG CAGCAGAACT GCATCCTGGT 
GGCAAGGACG GAGAGCTTCA TGTTGCTGAG CATCCTGCAG GACATCTGGT TCTCAAATGG 
TTACTAGAGC AAGATAAAAA GATGAAAGAA AGCGGAAAGG AAGGTTGTTT TGCAAAAACC 
CTTGTAGAGC GTGTTGGTAT GAAGAACCTC AAGTCCTGGG CCAGCATCAA CCGAGGTGCC 
ATTATTCTTT CCAGCCTTCT TCAGAGTTGT GACCAAGAAG TTGTAAACAA AGTCAAAGGT 
GGACTGAAAC CCCTGATTCC TACCTTGGAA AAAAACAAAA GCAGCAGCAG AGGCATCCAG 
ACCCTGCTTG AGAAGCTGAC TGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527246.1
CDS	393..2339
Translation	MMEVKGKKKFTGKSPQTSQGKNKFHKNSESSSSKTFPRKAVKEGGPKVTSKNFEKGATKPGKKGVKQFKNKPQGGKGPKDKFQKANKFSKKRKFQPDGESDESGAKKPKWDDFKKKKKELKQSRQLSDKTNYDIVVRAKHIWESLRRKDCDKEKRVKLMSDLQKLIQGKIKTIAFAHDSTRVIQCFIQYGNEEQRKQAFQELQGDLVELSKAKYSRNIVKKFLMYGSKPQVAEIIRSFKGHVRKMLRHSEASAIVEYAYNDKAILEQRNMLTEELYGNTFQLYKSADHPTLDKVLELQPAKLELIMDEMKQILTPMAQKEAVIKHSLVHKVFLDFFTYAPPKPRSELIEAIREAVVYLAHTHDGARVAMHCLWHGTPKDRKVIVKTMKTYVEKVANGQYSHLVLLAAFDCIDDTKLVKQIIISEIISSLPSIVNDKYGRKVLLYLMSPRDPAHTVPELIELLQKGDGNAHSKKDTAIRRRELLESISPALLSYLQGHTQEVVLDKSACVLVSDMLGSATGDVQPAMDAIASLAAAELHPGGKDGELHVAEHPAGHLVLKWLLEQDKKMKESGKEGCFAKTLVERVGMKNLKSWASINRGAIILSSLLQSCDQEVVNKVKGGLKPLIPTLEKNKSSSRGIQTLLEKLTA

Target ORF information:

RefSeq Version	XM_006527183.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus pumilio RNA-binding family member 3 (Pum3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527183.3

ORF Insert Sequence:

ATGATGGAAG TCAAAGGGAA GAAAAAGTTT ACAGGAAAGA GTCCACAGAC ATCACAAGGA 
AAGAATAAAT TTCATAAGAA CAGTGAGTCT AGTTCTTCAA AGACATTCCC AAGGAAAGCT 
GTTAAGGAAG GCGGACCTAA AGTCACATCT AAGAACTTTG AGAAAGGTGC CACAAAACCT 
GGCAAGAAAG GTGTGAAGCA GTTCAAGAAC AAGCCACAAG GGGGCAAAGG ACCGAAGGAC 
AAATTTCAGA AGGCAAATAA ATTCAGCAAG AAGCGGAAAT TCCAGCCAGA TGGTGAAAGT 
GATGAATCAG GAGCCAAGAA ACCCAAGTGG GATGACTTCA AAAAGAAGAA GAAAGAGCTG 
AAGCAGAGCA GGCAGCTCAG TGACAAGACC AACTATGACA TCGTGGTTCG AGCAAAGCAC 
ATTTGGGAGA GCTTGAGAAG AAAAGATTGT GACAAGGAAA AACGGGTGAA GCTCATGAGT 
GATTTGCAGA AGTTGATTCA AGGGAAAATT AAAACTATCG CCTTTGCACA TGACTCGACG 
CGCGTGATCC AGTGCTTCAT TCAGTATGGG AACGAGGAGC AGAGGAAGCA GGCTTTCCAA 
GAGTTGCAAG GCGACTTGGT TGAATTAAGT AAAGCCAAAT ATTCCAGGAA TATCGTTAAG 
AAATTTCTCA TGTATGGAAG TAAGCCACAA GTTGCGGAGA TAATCAGAAG TTTTAAAGGT 
CATGTGAGGA AGATGCTGCG GCATTCGGAG GCATCGGCCA TTGTGGAGTA CGCATACAAC 
GACAAAGCCA TTTTGGAACA GAGGAACATG CTGACAGAGG AGCTCTATGG GAACACGTTT 
CAGCTTTACA AGTCAGCAGA TCACCCAACG CTGGACAAGG TGCTGGAGCT GCAGCCAGCA 
AAGCTAGAGC TTATCATGGA CGAGATGAAG CAGATTCTGA CACCGATGGC CCAAAAGGAA 
GCTGTGATTA AGCACTCACT CGTTCATAAA GTATTCTTGG ACTTTTTTAC CTACGCACCC 
CCAAAACCTC GATCAGAACT AATTGAAGCC ATCCGGGAAG CAGTGGTGTA CCTGGCCCAC 
ACACACGATG GCGCCAGAGT GGCCATGCAC TGCCTGTGGC ACGGCACACC CAAGGACAGG 
AAAGTGATCG TGAAAACGAT GAAGACGTAT GTGGAGAAGG TTGCCAATGG CCAGTACTCT 
CACTTGGTTC TCCTGGCAGC GTTTGACTGT ATTGATGATA CTAAGCTTGT GAAGCAGATA 
ATCATATCAG AAATCATCAG TTCCTTGCCC AGCATAGTTA ATGACAAATA CGGAAGGAAA 
GTCCTCTTGT ACTTGATGAG CCCCAGAGAC CCCGCCCACA CGGTGCCCGA GCTCATCGAG 
CTTCTGCAGA AGGGTGATGG CAACGCGCAC AGCAAGAAGG ACACTGCAAT CCGCCGGCGT 
GAGCTCTTGG AGTCCATCTC TCCAGCTTTG CTGAGCTACC TGCAAGGACA CACTCAAGAG 
GTGGTGCTGG ATAAGTCTGC ATGTGTGCTG GTGTCTGACA TGCTGGGATC TGCTACTGGA 
GATGTTCAGC CTGCTATGGA TGCCATCGCC AGTTTGGCAG CAGCAGAACT GCATCCTGGT 
GGCAAGGACG GAGAGCTTCA TGTTGCTGAG CATCCTGCAG GACATCTGGT TCTCAAATGG 
TTACTAGAGC AAGATAAAAA GATGAAAGAA AGCGGAAAGG AAGGTTGTTT TGCAAAAACC 
CTTGTAGAGC GTGTTGGTAT GAAGAACCTC AAGTCCTGGG CCAGCATCAA CCGAGGTGCC 
ATTATTCTTT CCAGCCTTCT TCAGAGTTGT GACCAAGAAG TTGTAAACAA AGTCAAAGGT 
GGACTGAAAC CCCTGATTCC TACCTTGGAA AAAAACAAAA GCAGCAGCAG AGGCATCCAG 
ACCCTGCTTG AGAAGCTGAC TGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pum3 ( XM_006527183.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527246.1 Mus musculus pumilio RNA-binding family member 3 (Pum3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu23715
Clone ID Related Accession (Same CDS sequence)	XM_006527183.3 , XM_006527183.4 , NM_177474.5
Accession Version	XM_006527183.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	pumilio homolog 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527183.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG TCAAAGGGAA GAAAAAGTTT ACAGGAAAGA GTCCACAGAC ATCACAAGGA 
AAGAATAAAT TTCATAAGAA CAGTGAGTCT AGTTCTTCAA AGACATTCCC AAGGAAAGCT 
GTTAAGGAAG GCGGACCTAA AGTCACATCT AAGAACTTTG AGAAAGGTGC CACAAAACCT 
GGCAAGAAAG GTGTGAAGCA GTTCAAGAAC AAGCCACAAG GGGGCAAAGG ACCGAAGGAC 
AAATTTCAGA AGGCAAATAA ATTCAGCAAG AAGCGGAAAT TCCAGCCAGA TGGTGAAAGT 
GATGAATCAG GAGCCAAGAA ACCCAAGTGG GATGACTTCA AAAAGAAGAA GAAAGAGCTG 
AAGCAGAGCA GGCAGCTCAG TGACAAGACC AACTATGACA TCGTGGTTCG AGCAAAGCAC 
ATTTGGGAGA GCTTGAGAAG AAAAGATTGT GACAAGGAAA AACGGGTGAA GCTCATGAGT 
GATTTGCAGA AGTTGATTCA AGGGAAAATT AAAACTATCG CCTTTGCACA TGACTCGACG 
CGCGTGATCC AGTGCTTCAT TCAGTATGGG AACGAGGAGC AGAGGAAGCA GGCTTTCCAA 
GAGTTGCAAG GCGACTTGGT TGAATTAAGT AAAGCCAAAT ATTCCAGGAA TATCGTTAAG 
AAATTTCTCA TGTATGGAAG TAAGCCACAA GTTGCGGAGA TAATCAGAAG TTTTAAAGGT 
CATGTGAGGA AGATGCTGCG GCATTCGGAG GCATCGGCCA TTGTGGAGTA CGCATACAAC 
GACAAAGCCA TTTTGGAACA GAGGAACATG CTGACAGAGG AGCTCTATGG GAACACGTTT 
CAGCTTTACA AGTCAGCAGA TCACCCAACG CTGGACAAGG TGCTGGAGCT GCAGCCAGCA 
AAGCTAGAGC TTATCATGGA CGAGATGAAG CAGATTCTGA CACCGATGGC CCAAAAGGAA 
GCTGTGATTA AGCACTCACT CGTTCATAAA GTATTCTTGG ACTTTTTTAC CTACGCACCC 
CCAAAACCTC GATCAGAACT AATTGAAGCC ATCCGGGAAG CAGTGGTGTA CCTGGCCCAC 
ACACACGATG GCGCCAGAGT GGCCATGCAC TGCCTGTGGC ACGGCACACC CAAGGACAGG 
AAAGTGATCG TGAAAACGAT GAAGACGTAT GTGGAGAAGG TTGCCAATGG CCAGTACTCT 
CACTTGGTTC TCCTGGCAGC GTTTGACTGT ATTGATGATA CTAAGCTTGT GAAGCAGATA 
ATCATATCAG AAATCATCAG TTCCTTGCCC AGCATAGTTA ATGACAAATA CGGAAGGAAA 
GTCCTCTTGT ACTTGATGAG CCCCAGAGAC CCCGCCCACA CGGTGCCCGA GCTCATCGAG 
CTTCTGCAGA AGGGTGATGG CAACGCGCAC AGCAAGAAGG ACACTGCAAT CCGCCGGCGT 
GAGCTCTTGG AGTCCATCTC TCCAGCTTTG CTGAGCTACC TGCAAGGACA CACTCAAGAG 
GTGGTGCTGG ATAAGTCTGC ATGTGTGCTG GTGTCTGACA TGCTGGGATC TGCTACTGGA 
GATGTTCAGC CTGCTATGGA TGCCATCGCC AGTTTGGCAG CAGCAGAACT GCATCCTGGT 
GGCAAGGACG GAGAGCTTCA TGTTGCTGAG CATCCTGCAG GACATCTGGT TCTCAAATGG 
TTACTAGAGC AAGATAAAAA GATGAAAGAA AGCGGAAAGG AAGGTTGTTT TGCAAAAACC 
CTTGTAGAGC GTGTTGGTAT GAAGAACCTC AAGTCCTGGG CCAGCATCAA CCGAGGTGCC 
ATTATTCTTT CCAGCCTTCT TCAGAGTTGT GACCAAGAAG TTGTAAACAA AGTCAAAGGT 
GGACTGAAAC CCCTGATTCC TACCTTGGAA AAAAACAAAA GCAGCAGCAG AGGCATCCAG 
ACCCTGCTTG AGAAGCTGAC TGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527246.1
CDS	435..2381
Translation	MMEVKGKKKFTGKSPQTSQGKNKFHKNSESSSSKTFPRKAVKEGGPKVTSKNFEKGATKPGKKGVKQFKNKPQGGKGPKDKFQKANKFSKKRKFQPDGESDESGAKKPKWDDFKKKKKELKQSRQLSDKTNYDIVVRAKHIWESLRRKDCDKEKRVKLMSDLQKLIQGKIKTIAFAHDSTRVIQCFIQYGNEEQRKQAFQELQGDLVELSKAKYSRNIVKKFLMYGSKPQVAEIIRSFKGHVRKMLRHSEASAIVEYAYNDKAILEQRNMLTEELYGNTFQLYKSADHPTLDKVLELQPAKLELIMDEMKQILTPMAQKEAVIKHSLVHKVFLDFFTYAPPKPRSELIEAIREAVVYLAHTHDGARVAMHCLWHGTPKDRKVIVKTMKTYVEKVANGQYSHLVLLAAFDCIDDTKLVKQIIISEIISSLPSIVNDKYGRKVLLYLMSPRDPAHTVPELIELLQKGDGNAHSKKDTAIRRRELLESISPALLSYLQGHTQEVVLDKSACVLVSDMLGSATGDVQPAMDAIASLAAAELHPGGKDGELHVAEHPAGHLVLKWLLEQDKKMKESGKEGCFAKTLVERVGMKNLKSWASINRGAIILSSLLQSCDQEVVNKVKGGLKPLIPTLEKNKSSSRGIQTLLEKLTA

Target ORF information:

RefSeq Version	XM_006527183.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus pumilio RNA-binding family member 3 (Pum3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527183.4

ORF Insert Sequence:

ATGATGGAAG TCAAAGGGAA GAAAAAGTTT ACAGGAAAGA GTCCACAGAC ATCACAAGGA 
AAGAATAAAT TTCATAAGAA CAGTGAGTCT AGTTCTTCAA AGACATTCCC AAGGAAAGCT 
GTTAAGGAAG GCGGACCTAA AGTCACATCT AAGAACTTTG AGAAAGGTGC CACAAAACCT 
GGCAAGAAAG GTGTGAAGCA GTTCAAGAAC AAGCCACAAG GGGGCAAAGG ACCGAAGGAC 
AAATTTCAGA AGGCAAATAA ATTCAGCAAG AAGCGGAAAT TCCAGCCAGA TGGTGAAAGT 
GATGAATCAG GAGCCAAGAA ACCCAAGTGG GATGACTTCA AAAAGAAGAA GAAAGAGCTG 
AAGCAGAGCA GGCAGCTCAG TGACAAGACC AACTATGACA TCGTGGTTCG AGCAAAGCAC 
ATTTGGGAGA GCTTGAGAAG AAAAGATTGT GACAAGGAAA AACGGGTGAA GCTCATGAGT 
GATTTGCAGA AGTTGATTCA AGGGAAAATT AAAACTATCG CCTTTGCACA TGACTCGACG 
CGCGTGATCC AGTGCTTCAT TCAGTATGGG AACGAGGAGC AGAGGAAGCA GGCTTTCCAA 
GAGTTGCAAG GCGACTTGGT TGAATTAAGT AAAGCCAAAT ATTCCAGGAA TATCGTTAAG 
AAATTTCTCA TGTATGGAAG TAAGCCACAA GTTGCGGAGA TAATCAGAAG TTTTAAAGGT 
CATGTGAGGA AGATGCTGCG GCATTCGGAG GCATCGGCCA TTGTGGAGTA CGCATACAAC 
GACAAAGCCA TTTTGGAACA GAGGAACATG CTGACAGAGG AGCTCTATGG GAACACGTTT 
CAGCTTTACA AGTCAGCAGA TCACCCAACG CTGGACAAGG TGCTGGAGCT GCAGCCAGCA 
AAGCTAGAGC TTATCATGGA CGAGATGAAG CAGATTCTGA CACCGATGGC CCAAAAGGAA 
GCTGTGATTA AGCACTCACT CGTTCATAAA GTATTCTTGG ACTTTTTTAC CTACGCACCC 
CCAAAACCTC GATCAGAACT AATTGAAGCC ATCCGGGAAG CAGTGGTGTA CCTGGCCCAC 
ACACACGATG GCGCCAGAGT GGCCATGCAC TGCCTGTGGC ACGGCACACC CAAGGACAGG 
AAAGTGATCG TGAAAACGAT GAAGACGTAT GTGGAGAAGG TTGCCAATGG CCAGTACTCT 
CACTTGGTTC TCCTGGCAGC GTTTGACTGT ATTGATGATA CTAAGCTTGT GAAGCAGATA 
ATCATATCAG AAATCATCAG TTCCTTGCCC AGCATAGTTA ATGACAAATA CGGAAGGAAA 
GTCCTCTTGT ACTTGATGAG CCCCAGAGAC CCCGCCCACA CGGTGCCCGA GCTCATCGAG 
CTTCTGCAGA AGGGTGATGG CAACGCGCAC AGCAAGAAGG ACACTGCAAT CCGCCGGCGT 
GAGCTCTTGG AGTCCATCTC TCCAGCTTTG CTGAGCTACC TGCAAGGACA CACTCAAGAG 
GTGGTGCTGG ATAAGTCTGC ATGTGTGCTG GTGTCTGACA TGCTGGGATC TGCTACTGGA 
GATGTTCAGC CTGCTATGGA TGCCATCGCC AGTTTGGCAG CAGCAGAACT GCATCCTGGT 
GGCAAGGACG GAGAGCTTCA TGTTGCTGAG CATCCTGCAG GACATCTGGT TCTCAAATGG 
TTACTAGAGC AAGATAAAAA GATGAAAGAA AGCGGAAAGG AAGGTTGTTT TGCAAAAACC 
CTTGTAGAGC GTGTTGGTAT GAAGAACCTC AAGTCCTGGG CCAGCATCAA CCGAGGTGCC 
ATTATTCTTT CCAGCCTTCT TCAGAGTTGT GACCAAGAAG TTGTAAACAA AGTCAAAGGT 
GGACTGAAAC CCCTGATTCC TACCTTGGAA AAAAACAAAA GCAGCAGCAG AGGCATCCAG 
ACCCTGCTTG AGAAGCTGAC TGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A divergent Pumilio repeat protein family for pre-rRNA processing and mRNA localization.
Proceedings of the National Academy of Sciences of the United States of America111(52)18554-9(2014 Dec)
Qiu C,McCann KL,Wine RN,Baserga SJ,Hall TM

A comparative study of protein carbonylation and mitochondrial dysfunction using the neurotoxicants 1,3-dinitrobenzene, 3-nitropropionic acid, and 3-chloropropanediol.
Neurotoxicology3774-84(2013 Jul)
Steiner SR,Milton E,Philbert MA

A novel puf-A gene predicted from evolutionary analysis is involved in the development of eyes and primordial germ-cells.
PloS one4(3)e4980(2009)
Kuo MW,Wang SH,Chang JC,Chang CH,Huang LJ,Lin HH,Yu AL,Li WH,Yu J

Testicular germ cell tumor susceptibility genes from the consomic 129.MOLF-Chr19 mouse strain.
Mammalian genome : official journal of the International Mammalian Genome Society18(8)584-95(2007 Aug)
Zhu R,Ji Y,Xiao L,Matin A