Clec4a4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Clec4a4
Entrez Gene ID	474145
Full Name	C-type lectin domain family 4, member a4
Synonyms	Dcir2
General protein information	Preferred Names C-type lectin domain family 4, member a4 Names C-type lectin domain family 4, member a4 dendritic cell inhibitory receptor 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 F2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001005860.2	NP_001005860.1	C-type lectin domain family 4, member a4

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Clec4a2	NP_001005880.1
Mus musculus (house mouse)	Clec4a4	NP_001005860.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Clec4A4 is a regulatory receptor for dendritic cells that impairs inflammation and T-cell immunity.
Uto T, Fukaya T, Takagi H, Arimura K, Nakamura T, Kojima N, Malissen B, Sato K
Nature communications711273(2016 Apr)

DCIR2+ cDC2 DCs and Zbtb32 Restore CD4+ T-Cell Tolerance and Inhibit Diabetes.
Price JD, Hotta-Iwamura C, Zhao Y, Beauchamp NM, Tarbell KV
Diabetes64(10)3521-31(2015 Oct)

Recognition of bisecting N-acetylglucosamine: structural basis for asymmetric interaction with the mouse lectin dendritic cell inhibitory receptor 2.
Nagae M, Yamanaka K, Hanashima S, Ikeda A, Morita-Matsumoto K, Satoh T, Matsumoto N, Yamamoto K, Yamaguchi Y
The Journal of biological chemistry288(47)33598-610(2013 Nov)

Identification of lectin-like receptors expressed by antigen presenting cells and neutrophils and their mapping to a novel gene complex.
Flornes LM, Bryceson YT, Spurkland A, Lorentzen JC, Dissen E, Fossum S
Immunogenetics56(7)506-17(2004 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings highlight roles of Clec4A4 in regulation of the function of CD8alpha(-) conventional dendritic cells for control of the magnitude and quality of immune response.
Title: Clec4A4 is a regulatory receptor for dendritic cells that impairs inflammation and T-cell immunity.

This study showed that DCIR2(+) DCs are capable of inducing antigen-specific tolerance in the face of ongoing autoimmunity; Zbtb32 is a suppressive transcription factor that controls T cell-mediated autoimmunity.
Title: DCIR2+ cDC2 DCs and Zbtb32 Restore CD4+ T-Cell Tolerance and Inhibit Diabetes.

the unique binding mode of mDCIR2 for bisecting GlcNAc-containing glycans, a mode that contrasts sharply with that of other immune C-type lectin receptors such as DC-SIGN.
Title: Recognition of bisecting N-acetylglucosamine: structural basis for asymmetric interaction with the mouse lectin dendritic cell inhibitory receptor 2.

The following Clec4a4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Clec4a4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu10719	NM_001005860.2 Latest version!	Mus musculus C-type lectin domain family 4, member a4 (Clec4a4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Clec4a4 ( NM_001005860.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001005860.1 Mus musculus C-type lectin domain family 4, member a4 (Clec4a4), mRNA.

Price & Availability↑

CloneID	OMu10719
Clone ID Related Accession (Same CDS sequence)	NM_001005860.2
Accession Version	NM_001005860.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-25
Organism	Mus musculus(house mouse)
Product	C-type lectin domain family 4, member a4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY397673.1 and BX524711.1. On Feb 24, 2011 this sequence version replaced NM_001005860.1. ##Evidence-Data-START## Transcript exon combination :: AY397673.1, BC104370.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849388 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTCAG AAATCACTTA TGCAGAAGTG AGGATCACGA ATGAATCCGA CTCCTTGGAC 
ACCTACTCAA AATGTCCTGC AGCTCCCAGA GAGAAACCTA TCCGTGATCT AAGAAAGCCT 
GGTTCCCCCT CACTGCTTCT TACATCCCTG ATGCTACTTC TCCTGCTGCT GGCAATCACA 
TTCTTAGTTG CTTTTATCAT TTATTTTCAA AAGTACTCTC AACTTCTTGA AGAAAAAGAA 
GCTGCAAAAA ATATAATGTA CAAGGAATTG AACTGCATAA AAAATGGTTC ACTCATGGAA 
GACAAAGTCT GGAGCTGTTG CCCAAAGGAT TGGAAACCAT TTGTTTCCCA CTGCTACTTC 
ATTTTGAATG ACTCGAAGGC ATCTTGGAAT GAGAGTGAGG AGAAGTGCTC CCACATGGGT 
GCTCATCTGG TGGTGATCCA CAGCCAGGCA GAGCAGGATT TCATCACCAG CAACCTGAAC 
ACAAGTGCTG GTTATTTTAT AGGACTTTTG GATGCTGGTC AAAGACAATG GCGATGGATT 
GATCAGACAC CATACAATAA GAGTGCTACG TTCTGGCACA AAGGTGAGCC CAACCAAGAT 
TGGGAACGAT GTGTTATAAT AAATCATAAA ACAACTGGAT GGGGCTGGAA TGATATCCCT 
TGCAAAGATG AACACAATTC AGTTTGTCAG GTGAAGAAAA TATACTTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005860.1
CDS	1..711
Translation	MASEITYAEVRITNESDSLDTYSKCPAAPREKPIRDLRKPGSPSLLLTSLMLLLLLLAITFLVAFIIYFQKYSQLLEEKEAAKNIMYKELNCIKNGSLMEDKVWSCCPKDWKPFVSHCYFILNDSKASWNESEEKCSHMGAHLVVIHSQAEQDFITSNLNTSAGYFIGLLDAGQRQWRWIDQTPYNKSATFWHKGEPNQDWERCVIINHKTTGWGWNDIPCKDEHNSVCQVKKIYL

Target ORF information:

RefSeq Version	NM_001005860.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus C-type lectin domain family 4, member a4 (Clec4a4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005860.2

ORF Insert Sequence:

ATGGCTTCAG AAATCACTTA TGCAGAAGTG AGGATCACGA ATGAATCCGA CTCCTTGGAC 
ACCTACTCAA AATGTCCTGC AGCTCCCAGA GAGAAACCTA TCCGTGATCT AAGAAAGCCT 
GGTTCCCCCT CACTGCTTCT TACATCCCTG ATGCTACTTC TCCTGCTGCT GGCAATCACA 
TTCTTAGTTG CTTTTATCAT TTATTTTCAA AAGTACTCTC AACTTCTTGA AGAAAAAGAA 
GCTGCAAAAA ATATAATGTA CAAGGAATTG AACTGCATAA AAAATGGTTC ACTCATGGAA 
GACAAAGTCT GGAGCTGTTG CCCAAAGGAT TGGAAACCAT TTGTTTCCCA CTGCTACTTC 
ATTTTGAATG ACTCGAAGGC ATCTTGGAAT GAGAGTGAGG AGAAGTGCTC CCACATGGGT 
GCTCATCTGG TGGTGATCCA CAGCCAGGCA GAGCAGGATT TCATCACCAG CAACCTGAAC 
ACAAGTGCTG GTTATTTTAT AGGACTTTTG GATGCTGGTC AAAGACAATG GCGATGGATT 
GATCAGACAC CATACAATAA GAGTGCTACG TTCTGGCACA AAGGTGAGCC CAACCAAGAT 
TGGGAACGAT GTGTTATAAT AAATCATAAA ACAACTGGAT GGGGCTGGAA TGATATCCCT 
TGCAAAGATG AACACAATTC AGTTTGTCAG GTGAAGAAAA TATACTTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Clec4A4 is a regulatory receptor for dendritic cells that impairs inflammation and T-cell immunity.
Nature communications711273(2016 Apr)
Uto T,Fukaya T,Takagi H,Arimura K,Nakamura T,Kojima N,Malissen B,Sato K

DCIR2+ cDC2 DCs and Zbtb32 Restore CD4+ T-Cell Tolerance and Inhibit Diabetes.
Diabetes64(10)3521-31(2015 Oct)
Price JD,Hotta-Iwamura C,Zhao Y,Beauchamp NM,Tarbell KV

Recognition of bisecting N-acetylglucosamine: structural basis for asymmetric interaction with the mouse lectin dendritic cell inhibitory receptor 2.
The Journal of biological chemistry288(47)33598-610(2013 Nov)
Nagae M,Yamanaka K,Hanashima S,Ikeda A,Morita-Matsumoto K,Satoh T,Matsumoto N,Yamamoto K,Yamaguchi Y

Identification of lectin-like receptors expressed by antigen presenting cells and neutrophils and their mapping to a novel gene complex.
Immunogenetics56(7)506-17(2004 Oct)
Flornes LM,Bryceson YT,Spurkland A,Lorentzen JC,Dissen E,Fossum S