Tcaf3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tcaf3
Entrez Gene ID	403088
Full Name	TRPM8 channel-associated factor 3
Synonyms	Eapa2,Fam115e
General protein information	Preferred Names TRPM8 channel-associated factor 3 Names TRPM8 channel-associated factor 3 experimental autoimmune prostatitis antigen 2 family with sequence similarity 115, member E protein FAM115E
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 B2.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_203396.1	NP_981933.1	TRPM8 channel-associated factor 3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Fam115e	NP_981933.1
Rattus norvegicus (Norway rat)	Fam115e	NP_001009534.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of Natural Regulatory T Cell Epitopes Reveals Convergence on a Dominant Autoantigen.
Leonard JD, Gilmore DC, Dileepan T, Nawrocka WI, Chao JL, Schoenbach MH, Jenkins MK, Adams EJ, Savage PA
Immunity47(1)107-117.e8(2017 07)

TRP channel-associated factors are a novel protein family that regulates TRPM8 trafficking and activity.
Gkika D, Lemonnier L, Shapovalov G, Gordienko D, Poux C, Bernardini M, Bokhobza A, Bidaux G, Degerny C, Verreman K, Guarmit B, Benahmed M, de Launoit Y, Bindels RJ, Fiorio Pla A, Prevarskaya N
The Journal of cell biology208(1)89-107(2015 Jan)

Physiologic self antigens rapidly capacitate autoimmune disease-specific polyclonal CD4+ CD25+ regulatory T cells.
Setiady YY, Ohno K, Samy ET, Bagavant H, Qiao H, Sharp C, She JX, Tung KS
Blood107(3)1056-62(2006 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tcaf3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tcaf3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu58136	NM_203396.1 Latest version!	Mus musculus TRPM8 channel-associated factor 3 (Tcaf3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tcaf3 ( NM_203396.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_981933.1 Mus musculus TRPM8 channel-associated factor 3 (Tcaf3), mRNA.

Price & Availability↑

CloneID	OMu58136
Clone ID Related Accession (Same CDS sequence)	NM_203396.1
Accession Version	NM_203396.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Mus musculus(house mouse)
Product	TRPM8 channel-associated factor 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY528666.1. ##Evidence-Data-START## Transcript exon combination :: AY528666.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCAACAA CCCCTGATGC TGCTTTTGAG ACCCTCATGA ATGGAGTGAC CAGCTGGGAT 
CTTCCCAAAG AACCCATTCC CAGTGAACTT CTCCTTACTG GGGAGTCAGC CTTCCCAGTG 
ATGGTGAATG ACAAGGGCCA GGTGCTCATT GCTGCCTCTT CCTATGGACA AGGCCGCCTG 
GTGGTCGTAT CCCATGAGAG CTACCTGCTG CACGATGGCT TGGTTCCATT TCTTCTCAAT 
GTGGTGAAAT GGCTCTGCCC CTGCCCAGGG GCTCCCATAG CGGTGCATTC TTCCTTAGCA 
TCATTAGTTA ACATCCTAGG TGATTCTGGG ATAAATGCCT TGGTTCAGCC TGAGCCTGGA 
GAGGCTCTGG GAGTTTATTG CATTGATGCC TACAATGATG CCTTGACTGA GAAGCTGATC 
CAATTCCTGA AGAATGGAGG AGGTTTGCTA ATTGGAGGCC AGGCTTTGAA TTGGGCTGCT 
CACCATGGCC ATGATAAAGT GCTATCCATT TTTCCTGGAA ACCAGGTGAC AAGTGTGGCT 
GGAGTATATT TCACTGATAT CTCTGCGAAT AGAGACTGGT TCAAGGTTTC TAAGGAGATA 
CCCAATCTCA GACTCTATGT CCAGTGTGAG GATGAGCTCG AGGACGACCA GCAGCAGCTC 
CTGAAAGGGA TGTCTGAGAT ATACATCGAG GCGGGTGTAA TCCCCTCCCA GCTGCTTGTG 
CATGGACAGC GGGCATTTCC CTTGGGAGTA GACAATTCAC TCAATTGCTT TCTTGCAGCT 
GCCCGCTATG GCCGTGGTCG GGTGGTGCTG GGGGGAAATG AGAGTCTCAT ACTTAATCAA 
ACTATGCTGC CATTTGTGCT TAATGCTTTA CACTGGCTGA TGGGGAACCA GACAGGCAGA 
ATTGGGTTAG CTTCAGATAT GAAAGTACTG AAGTCTATGT TACCAAACAG CAGTTTCCAG 
TGGAGTGAAT CAGAACTTCT AACCAGTGAC TTGAGCGTGT TTTGCTGCTG CTCACTCGCC 
AATATAGATT CCGAGGAGGT AGAGGAATTT GTTGCAGAAG GTGGAGGCTT GCTAATTGGT 
GCAGAGGCCT GGTCGTGGGG CCGCAGGAAT CCATACTCTA GCTGTATGAC TCAATACCCA 
GATAACATCG TCCTTAAGCG CTTTGGCCTT GGTATCACTA GCCATGTGGC CCAGCGAGGC 
AGTTTCCCAT TCCCCAATCC TGAAGGGACA AACTATCACT TTCGGAGAGC CCTGTCTCAA 
TTTGAGTCCG TGATCTACAG CCGGGGGAGT AGCTTACACG AGAGCTGGCT GAATAAGCTC 
TCACAAGATT GTTTCTACAT GTTTCAAATG ACCCATCAGA GGATTTCCAT CTACGACTCT 
GTGAAGAAAC ATGCTCTGAA AATGATCCAA AGCAAGGACT TTCCAAGTGT GACTGAACAA 
TACCCGATCG CAAGGGGCAG CTCACAGGCT TTTCTTCTAT CCCTGGCTTA TGAATTATTC 
AAATCCGGAG TTGACAGATC TCAGCTACTT CCCCCTCCCG CCTTACTACC TCCTACAGAA 
TCTCCAATAA CCATTAAAAT AAGCACAGAC AATGACAACT CCTGGGTGAG CACTGGACTG 
TACTTACCTG AAGGGCAGGT TGCACAAGTA TTACTTCCCA GTGAAGCTAC TCATGCCAAG 
CTGAAGGTCC TCATTGGCTG CCACAGGGAC AACATAAGCC AAGCAAGAAC TTACTTCCGT 
CCCCCTGTGA TGACCTATGT GTACCACTTA ACTTCATCCC AGACATCCAT CTCCTGGCTC 
TATGGGGGCC TTCTCTACAT CATGGTCCCC AACAAATATA ATCAGGATAA TGTGTCTGTC 
ACCATCCGTG GGGCTGTATC TGCTCCATAC TTCAGGCTGG GTAAGACGAC CCAGGAGGAA 
TGGAAGAATC TTATCACACA CAGCAAAGCT CCGTGGGGAG AACTAGCCAC AGACAATATC 
ATCCTGACAA TTCCAACGGT AAACCTCAAG GAGCTTCAGG ACCCCTATCC ACTGCTCCAA 
CTCTGGGACA AGATGGTAAG GGCTGTAGCC AAGCTGGCAG CCCGGCCCTT CCCTTTTCAG 
AGAGCTGAGA GGGTCGTACT TGACAAGCAG ATTTCATTCG GTTTCCTTCA TTCTGGATAC 
CCCATCATGG GCCTAATTAG TATAGTAGAA GGCATCATCA GCGAGTTCAA AATAAGATCA 
CATGGCATTT GGGGTGTGAT ACATGAACTG GGCCACAACC ATCAGAAGTC TGGATGGACT 
TTTCCACCTC ACACCACCGA AGCTTTGTGC AATCTCTGGA CTATTTATGT GCATGAGACA 
GTTTTAAACA TCCCCAGGGA GCAAGCCCAT CCTTCTCTGA ATCCTGAGCT TAGGAGGCAG 
AGGATCAAAT ACCACCTGAA CAAGGGAGCA CCTCTGAGCA ACTGGATTAT GTGGACAGCT 
CTAGAAACTT ATCTCCAGCT CCAGGAGGGC TTTGGGTGGG AGCCATTCAT CCAGGTCTTT 
GCTGACTACC GAACCCTCTC TGGCCTTCCT CAAAACAATG AAGACAAGAT GAATCTGTGG 
GTGAAGAAGT TCTCTGAGGC TGTGCATAAG AATCTGGCAC CTTTCTTTGA AGCCTGGGGC 
TGGCCTGTCA AATATGCTGT GGCTAAAAGC CTAGCCTCTT TGCCAGAGTG GCAGGAAAAC 
CCCATGAAAA GGTATACAGC TGAAGGTACA GAAGGCAGAG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_981933.1
CDS	97..2841
Translation	MATTPDAAFETLMNGVTSWDLPKEPIPSELLLTGESAFPVMVNDKGQVLIAASSYGQGRLVVVSHESYLLHDGLVPFLLNVVKWLCPCPGAPIAVHSSLASLVNILGDSGINALVQPEPGEALGVYCIDAYNDALTEKLIQFLKNGGGLLIGGQALNWAAHHGHDKVLSIFPGNQVTSVAGVYFTDISANRDWFKVSKEIPNLRLYVQCEDELEDDQQQLLKGMSEIYIEAGVIPSQLLVHGQRAFPLGVDNSLNCFLAAARYGRGRVVLGGNESLILNQTMLPFVLNALHWLMGNQTGRIGLASDMKVLKSMLPNSSFQWSESELLTSDLSVFCCCSLANIDSEEVEEFVAEGGGLLIGAEAWSWGRRNPYSSCMTQYPDNIVLKRFGLGITSHVAQRGSFPFPNPEGTNYHFRRALSQFESVIYSRGSSLHESWLNKLSQDCFYMFQMTHQRISIYDSVKKHALKMIQSKDFPSVTEQYPIARGSSQAFLLSLAYELFKSGVDRSQLLPPPALLPPTESPITIKISTDNDNSWVSTGLYLPEGQVAQVLLPSEATHAKLKVLIGCHRDNISQARTYFRPPVMTYVYHLTSSQTSISWLYGGLLYIMVPNKYNQDNVSVTIRGAVSAPYFRLGKTTQEEWKNLITHSKAPWGELATDNIILTIPTVNLKELQDPYPLLQLWDKMVRAVAKLAARPFPFQRAERVVLDKQISFGFLHSGYPIMGLISIVEGIISEFKIRSHGIWGVIHELGHNHQKSGWTFPPHTTEALCNLWTIYVHETVLNIPREQAHPSLNPELRRQRIKYHLNKGAPLSNWIMWTALETYLQLQEGFGWEPFIQVFADYRTLSGLPQNNEDKMNLWVKKFSEAVHKNLAPFFEAWGWPVKYAVAKSLASLPEWQENPMKRYTAEGTEGRE

Target ORF information:

RefSeq Version	NM_203396.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus TRPM8 channel-associated factor 3 (Tcaf3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_203396.1

ORF Insert Sequence:

ATGGCAACAA CCCCTGATGC TGCTTTTGAG ACCCTCATGA ATGGAGTGAC CAGCTGGGAT 
CTTCCCAAAG AACCCATTCC CAGTGAACTT CTCCTTACTG GGGAGTCAGC CTTCCCAGTG 
ATGGTGAATG ACAAGGGCCA GGTGCTCATT GCTGCCTCTT CCTATGGACA AGGCCGCCTG 
GTGGTCGTAT CCCATGAGAG CTACCTGCTG CACGATGGCT TGGTTCCATT TCTTCTCAAT 
GTGGTGAAAT GGCTCTGCCC CTGCCCAGGG GCTCCCATAG CGGTGCATTC TTCCTTAGCA 
TCATTAGTTA ACATCCTAGG TGATTCTGGG ATAAATGCCT TGGTTCAGCC TGAGCCTGGA 
GAGGCTCTGG GAGTTTATTG CATTGATGCC TACAATGATG CCTTGACTGA GAAGCTGATC 
CAATTCCTGA AGAATGGAGG AGGTTTGCTA ATTGGAGGCC AGGCTTTGAA TTGGGCTGCT 
CACCATGGCC ATGATAAAGT GCTATCCATT TTTCCTGGAA ACCAGGTGAC AAGTGTGGCT 
GGAGTATATT TCACTGATAT CTCTGCGAAT AGAGACTGGT TCAAGGTTTC TAAGGAGATA 
CCCAATCTCA GACTCTATGT CCAGTGTGAG GATGAGCTCG AGGACGACCA GCAGCAGCTC 
CTGAAAGGGA TGTCTGAGAT ATACATCGAG GCGGGTGTAA TCCCCTCCCA GCTGCTTGTG 
CATGGACAGC GGGCATTTCC CTTGGGAGTA GACAATTCAC TCAATTGCTT TCTTGCAGCT 
GCCCGCTATG GCCGTGGTCG GGTGGTGCTG GGGGGAAATG AGAGTCTCAT ACTTAATCAA 
ACTATGCTGC CATTTGTGCT TAATGCTTTA CACTGGCTGA TGGGGAACCA GACAGGCAGA 
ATTGGGTTAG CTTCAGATAT GAAAGTACTG AAGTCTATGT TACCAAACAG CAGTTTCCAG 
TGGAGTGAAT CAGAACTTCT AACCAGTGAC TTGAGCGTGT TTTGCTGCTG CTCACTCGCC 
AATATAGATT CCGAGGAGGT AGAGGAATTT GTTGCAGAAG GTGGAGGCTT GCTAATTGGT 
GCAGAGGCCT GGTCGTGGGG CCGCAGGAAT CCATACTCTA GCTGTATGAC TCAATACCCA 
GATAACATCG TCCTTAAGCG CTTTGGCCTT GGTATCACTA GCCATGTGGC CCAGCGAGGC 
AGTTTCCCAT TCCCCAATCC TGAAGGGACA AACTATCACT TTCGGAGAGC CCTGTCTCAA 
TTTGAGTCCG TGATCTACAG CCGGGGGAGT AGCTTACACG AGAGCTGGCT GAATAAGCTC 
TCACAAGATT GTTTCTACAT GTTTCAAATG ACCCATCAGA GGATTTCCAT CTACGACTCT 
GTGAAGAAAC ATGCTCTGAA AATGATCCAA AGCAAGGACT TTCCAAGTGT GACTGAACAA 
TACCCGATCG CAAGGGGCAG CTCACAGGCT TTTCTTCTAT CCCTGGCTTA TGAATTATTC 
AAATCCGGAG TTGACAGATC TCAGCTACTT CCCCCTCCCG CCTTACTACC TCCTACAGAA 
TCTCCAATAA CCATTAAAAT AAGCACAGAC AATGACAACT CCTGGGTGAG CACTGGACTG 
TACTTACCTG AAGGGCAGGT TGCACAAGTA TTACTTCCCA GTGAAGCTAC TCATGCCAAG 
CTGAAGGTCC TCATTGGCTG CCACAGGGAC AACATAAGCC AAGCAAGAAC TTACTTCCGT 
CCCCCTGTGA TGACCTATGT GTACCACTTA ACTTCATCCC AGACATCCAT CTCCTGGCTC 
TATGGGGGCC TTCTCTACAT CATGGTCCCC AACAAATATA ATCAGGATAA TGTGTCTGTC 
ACCATCCGTG GGGCTGTATC TGCTCCATAC TTCAGGCTGG GTAAGACGAC CCAGGAGGAA 
TGGAAGAATC TTATCACACA CAGCAAAGCT CCGTGGGGAG AACTAGCCAC AGACAATATC 
ATCCTGACAA TTCCAACGGT AAACCTCAAG GAGCTTCAGG ACCCCTATCC ACTGCTCCAA 
CTCTGGGACA AGATGGTAAG GGCTGTAGCC AAGCTGGCAG CCCGGCCCTT CCCTTTTCAG 
AGAGCTGAGA GGGTCGTACT TGACAAGCAG ATTTCATTCG GTTTCCTTCA TTCTGGATAC 
CCCATCATGG GCCTAATTAG TATAGTAGAA GGCATCATCA GCGAGTTCAA AATAAGATCA 
CATGGCATTT GGGGTGTGAT ACATGAACTG GGCCACAACC ATCAGAAGTC TGGATGGACT 
TTTCCACCTC ACACCACCGA AGCTTTGTGC AATCTCTGGA CTATTTATGT GCATGAGACA 
GTTTTAAACA TCCCCAGGGA GCAAGCCCAT CCTTCTCTGA ATCCTGAGCT TAGGAGGCAG 
AGGATCAAAT ACCACCTGAA CAAGGGAGCA CCTCTGAGCA ACTGGATTAT GTGGACAGCT 
CTAGAAACTT ATCTCCAGCT CCAGGAGGGC TTTGGGTGGG AGCCATTCAT CCAGGTCTTT 
GCTGACTACC GAACCCTCTC TGGCCTTCCT CAAAACAATG AAGACAAGAT GAATCTGTGG 
GTGAAGAAGT TCTCTGAGGC TGTGCATAAG AATCTGGCAC CTTTCTTTGA AGCCTGGGGC 
TGGCCTGTCA AATATGCTGT GGCTAAAAGC CTAGCCTCTT TGCCAGAGTG GCAGGAAAAC 
CCCATGAAAA GGTATACAGC TGAAGGTACA GAAGGCAGAG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of Natural Regulatory T Cell Epitopes Reveals Convergence on a Dominant Autoantigen.
Immunity47(1)107-117.e8(2017 07)
Leonard JD,Gilmore DC,Dileepan T,Nawrocka WI,Chao JL,Schoenbach MH,Jenkins MK,Adams EJ,Savage PA

TRP channel-associated factors are a novel protein family that regulates TRPM8 trafficking and activity.
The Journal of cell biology208(1)89-107(2015 Jan)
Gkika D,Lemonnier L,Shapovalov G,Gordienko D,Poux C,Bernardini M,Bokhobza A,Bidaux G,Degerny C,Verreman K,Guarmit B,Benahmed M,de Launoit Y,Bindels RJ,Fiorio Pla A,Prevarskaya N

Physiologic self antigens rapidly capacitate autoimmune disease-specific polyclonal CD4+ CD25+ regulatory T cells.
Blood107(3)1056-62(2006 Feb)
Setiady YY,Ohno K,Samy ET,Bagavant H,Qiao H,Sharp C,She JX,Tung KS