Slirp cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slirp
Entrez Gene ID	380773
Full Name	SRA stem-loop interacting RNA binding protein
Synonyms	1810035L17Rik
General protein information	Preferred Names SRA stem-loop interacting RNA binding protein Names SRA stem-loop-interacting RNA-binding protein, mitochondrial
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 D2

mRNA and Protein(s)

mRNA	Protein	Name
NM_026958.3	NP_081234.2	SRA stem-loop-interacting RNA-binding protein, mitochondrial

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SLIRP	XP_003952595.1
Homo sapiens (human)	SLIRP	NP_112487.1
Canis lupus familiaris (dog)	SLIRP	XP_547932.2
Rattus norvegicus (Norway rat)	Slirp	NP_001102977.1
Danio rerio (zebrafish)	si:ch73-276n11.1	XP_002665211.1
Xenopus tropicalis (tropical clawed frog)	slirp	XP_002938905.2
Bos taurus (cattle)	SLIRP	NP_001032562.1
Mus musculus (house mouse)	Slirp	NP_081234.2
Gallus gallus (chicken)	SLIRP	XP_001235249.1

Gene Ontology
Bibliography

Related articles in PubMed

LRPPRC-mediated folding of the mitochondrial transcriptome.
Siira SJ, Sp?hr H, Shearwood AJ, Ruzzenente B, Larsson NG, Rackham O, Filipovska A
Nature communications8(1)1532(2017 Nov)

SLIRP Regulates the Rate of Mitochondrial Protein Synthesis and Protects LRPPRC from Degradation.
Lagouge M, Mourier A, Lee HJ, Sp?hr H, Wai T, Kukat C, Silva Ramos E, Motori E, Busch JD, Siira S, , Kremmer E, Filipovska A, Larsson NG
PLoS genetics11(8)e1005423(2015 Aug)

Loss of the nuclear receptor corepressor SLIRP compromises male fertility.
Colley SM, Wintle L, Searles R, Russell V, Firman RC, Smith S, Deboer K, Merriner DJ, Genevieve B, Bentel JM, Stuckey BG, Phillips MR, Simmons LW, de Kretser DM, O'Bryan MK, Leedman PJ
PloS one8(8)e70700(2013)

LRPPRC is necessary for polyadenylation and coordination of translation of mitochondrial mRNAs.
Ruzzenente B, Metodiev MD, Wredenberg A, Bratic A, Park CB, C?mara Y, Milenkovic D, Zickermann V, Wibom R, Hultenby K, Erdjument-Bromage H, Tempst P, Brandt U, Stewart JB, Gustafsson CM, Larsson NG
The EMBO journal31(2)443-56(2012 Jan)

A computational screen for regulators of oxidative phosphorylation implicates SLIRP in mitochondrial RNA homeostasis.
Baughman JM, Nilsson R, Gohil VM, Arlow DH, Gauhar Z, Mootha VK
PLoS genetics5(8)e1000590(2009 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

both LRPPRC and SLIRP are required for maintaining mt-mRNA steady-state levels independent of their roles in stabilizing the other partner of the LRPPRC-SLIRP complex.
Title: SLIRP Regulates the Rate of Mitochondrial Protein Synthesis and Protects LRPPRC from Degradation.

SLIRP regulates male fertility, wherein its loss results in asthenozoospermia associated with compromised sperm structure and mitochondrial morphology.
Title: Loss of the nuclear receptor corepressor SLIRP compromises male fertility.

SLIRP plays an essential role in maintaining mitochondrial-localized mRNA transcripts that encode OxPhos protein subunits.
Title: A computational screen for regulators of oxidative phosphorylation implicates SLIRP in mitochondrial RNA homeostasis.

The following Slirp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slirp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu17781	NM_026958.3 Latest version!	Mus musculus SRA stem-loop interacting RNA binding protein (Slirp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slirp ( NM_026958.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081234.2 Mus musculus SRA stem-loop interacting RNA binding protein (Slirp), mRNA.

Price & Availability↑

CloneID	OMu17781
Clone ID Related Accession (Same CDS sequence)	NM_026958.3
Accession Version	NM_026958.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	SRA stem-loop-interacting RNA-binding protein, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BU850988.1, BC116741.1 and CK372908.1. On Jan 5, 2010 this sequence version replaced NM_026958.2. ##Evidence-Data-START## Transcript exon combination :: AV462870.1, BU850369.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAGCCT CCGCCATAAA GGGGCTAAGC GCACTGCGTT CCAGTACTGG TCGGCCTATT 
GCGTTTGTCA GAAAAATTCC TTGGACCGCG GCAGCGAGTG AGCTGAGAGA ACATTTTGCA 
CAGTTTGGCC ATGTAAGAAG GTGCACTGTA CCTTTTGACA AAGAGACTGG CTTTCACAGA 
GGCATGGGTT GGGTTCAGTT TTCCTCTCAG GAAGAACTTC AGAATGCACT ACAACAAGAA 
CATCATATTA TTGATGGAGT AAAAATCCAC GTTCAAGCTC AAAGAGCAAA GGCTTTGCAC 
GGAGCTCAAA CATCTGATGA AGAAAGATTT CTGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081234.2
CDS	35..373
Translation	MAASAIKGLSALRSSTGRPIAFVRKIPWTAAASELREHFAQFGHVRRCTVPFDKETGFHRGMGWVQFSSQEELQNALQQEHHIIDGVKIHVQAQRAKALHGAQTSDEERFLR

Target ORF information:

RefSeq Version	NM_026958.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus SRA stem-loop interacting RNA binding protein (Slirp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026958.3

ORF Insert Sequence:

ATGGCAGCCT CCGCCATAAA GGGGCTAAGC GCACTGCGTT CCAGTACTGG TCGGCCTATT 
GCGTTTGTCA GAAAAATTCC TTGGACCGCG GCAGCGAGTG AGCTGAGAGA ACATTTTGCA 
CAGTTTGGCC ATGTAAGAAG GTGCACTGTA CCTTTTGACA AAGAGACTGG CTTTCACAGA 
GGCATGGGTT GGGTTCAGTT TTCCTCTCAG GAAGAACTTC AGAATGCACT ACAACAAGAA 
CATCATATTA TTGATGGAGT AAAAATCCAC GTTCAAGCTC AAAGAGCAAA GGCTTTGCAC 
GGAGCTCAAA CATCTGATGA AGAAAGATTT CTGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

LRPPRC-mediated folding of the mitochondrial transcriptome.
Nature communications8(1)1532(2017 Nov)
Siira SJ,Sp?hr H,Shearwood AJ,Ruzzenente B,Larsson NG,Rackham O,Filipovska A

SLIRP Regulates the Rate of Mitochondrial Protein Synthesis and Protects LRPPRC from Degradation.
PLoS genetics11(8)e1005423(2015 Aug)
Lagouge M,Mourier A,Lee HJ,Sp?hr H,Wai T,Kukat C,Silva Ramos E,Motori E,Busch JD,Siira S,,Kremmer E,Filipovska A,Larsson NG

Loss of the nuclear receptor corepressor SLIRP compromises male fertility.
PloS one8(8)e70700(2013)
Colley SM,Wintle L,Searles R,Russell V,Firman RC,Smith S,Deboer K,Merriner DJ,Genevieve B,Bentel JM,Stuckey BG,Phillips MR,Simmons LW,de Kretser DM,O'Bryan MK,Leedman PJ

LRPPRC is necessary for polyadenylation and coordination of translation of mitochondrial mRNAs.
The EMBO journal31(2)443-56(2012 Jan)
Ruzzenente B,Metodiev MD,Wredenberg A,Bratic A,Park CB,C?mara Y,Milenkovic D,Zickermann V,Wibom R,Hultenby K,Erdjument-Bromage H,Tempst P,Brandt U,Stewart JB,Gustafsson CM,Larsson NG

A computational screen for regulators of oxidative phosphorylation implicates SLIRP in mitochondrial RNA homeostasis.
PLoS genetics5(8)e1000590(2009 Aug)
Baughman JM,Nilsson R,Gohil VM,Arlow DH,Gauhar Z,Mootha VK