Agap1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Agap1
Entrez Gene ID	347722
Full Name	ArfGAP with GTPase domain, ankyrin repeat and PH domain 1
Synonyms	Centg2,Ggap1,R75364,mKIAA1099
General protein information	Preferred Names ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 Names arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 AGAP-1 centaurin-gamma-2 cnt-g2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 D

mRNA and Protein(s)

mRNA	Protein	Name
NM_178119.3	NP_835220.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform 1
NM_001037136.1	NP_001032213.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Agap1	NP_835220.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009160	XP_319921.4
Xenopus tropicalis (tropical clawed frog)	agap1	XP_002932044.1
Caenorhabditis elegans (roundworm)	cnt-2	NP_001022837.1
Gallus gallus (chicken)	AGAP1	XP_004942549.1
Drosophila melanogaster (fruit fly)	cenG1A	NP_723849.1
Canis lupus familiaris (dog)	AGAP1	XP_005635967.1
Homo sapiens (human)	AGAP1	NP_001032208.1
	AGAP1	XP_001082607.1
Rattus norvegicus (Norway rat)	Agap1	NP_001101700.1
Danio rerio (zebrafish)	agap1	XP_001921526.3

Gene Ontology
Bibliography

Related articles in PubMed

The Endosome Localized Arf-GAP AGAP1 Modulates Dendritic Spine Morphology Downstream of the Neurodevelopmental Disorder Factor Dysbindin.
Arnold M, Cross R, Singleton KS, Zlatic S, Chapleau C, Mullin AP, Rolle I, Moore CC, Theibert A, Pozzo-Miller L, Faundez V, Larimore J
Frontiers in cellular neuroscience10218(2016)

GTP-binding protein-like domain of AGAP1 is protein binding site that allosterically regulates ArfGAP protein catalytic activity.
Luo R, Akpan IO, Hayashi R, Sramko M, Barr V, Shiba Y, Randazzo PA
The Journal of biological chemistry287(21)17176-85(2012 May)

AGAP1/AP-3-dependent endocytic recycling of M5 muscarinic receptors promotes dopamine release.
Bendor J, Lizardi-Ortiz JE, Westphalen RI, Brandstetter M, Hemmings HC Jr, Sulzer D, Flajolet M, Greengard P
The EMBO journal29(16)2813-26(2010 Aug)

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Taniwaki T, Haruna K, Nakamura H, Sekimoto T, Oike Y, Imaizumi T, Saito F, Muta M, Soejima Y, Utoh A, Nakagata N, Araki M, Yamamura K, Araki K
Development, growth & differentiation47(3)163-72(2005 Apr)

AGAP1, a novel binding partner of nitric oxide-sensitive guanylyl cyclase.
Meurer S, Pioch S, Wagner K, M?ller-Esterl W, Gross S
The Journal of biological chemistry279(47)49346-54(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GTP-binding protein-like domain of AGAP1 is protein binding site that allosterically regulates ArfGAP protein catalytic activity.
Title: GTP-binding protein-like domain of AGAP1 is protein binding site that allosterically regulates ArfGAP protein catalytic activity.

The elimination of adaptor protein 3 or abrogation of AGAP1-M5 muscarinic receptor interaction in vivo decreased the magnitude of presynaptic M5 muscarinic receptor mediated dopamine release potentiation in the striatum.
Title: AGAP1/AP-3-dependent endocytic recycling of M5 muscarinic receptors promotes dopamine release.

AGAP1 is a novel binding partner of nitric oxide-sensitive guanylyl cyclase
Title: AGAP1, a novel binding partner of nitric oxide-sensitive guanylyl cyclase.

The following Agap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Agap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu07161	NM_178119.3 Latest version!	Mus musculus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu12816	NM_001037136.1 Latest version!	Mus musculus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Agap1 ( NM_178119.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_835220.1 Mus musculus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu07161
Clone ID Related Accession (Same CDS sequence)	NM_178119.3
Accession Version	NM_178119.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147503.1, AK046523.1 and AC124227.21. On or before Dec 7, 2005 this sequence version replaced XM_920295.1, XM_920309.1, XM_907301.1, NM_178119.2. Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: AK147503.1, AK046523.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTACC AGCAGCAGCT GGCCAACTCG GCTGCCATCC GGGCCGAGAT CCAGCGCTTC 
GAGTCGGTCC ACCCCAACAT CTACTCCATC TACGAGCTGC TGGAGCGCGT GGAGGAGCCG 
GTGCTGCAGA ACCAGATTCG GGAGCACGTC ATCGCCATCG AAGATGCTTT CGTGAACAGC 
CAGGAATGGA CACTCAGCCG ATCGGTACCG GAGCTCAAAG TGGGAATTGT GGGTAACCTG 
GCCAGTGGCA AGTCTGCGCT GGTGCACCGG TACCTGACAG GCACGTACGT GCAGGAGGAA 
TCTCCAGAAG GTGGCAGGTT CAAGAAGGAG ATTGTTGTTG ATGGACAGAG TTATCTGCTG 
CTGATTAGGG ATGAAGGGGG CCCCCCGGAG GCACAGTTCG CCATGTGGGT GGACGCGGTC 
ATCTTTGTCT TCAGCTTGGA GGATGAGATC AGTTTCCAGA CTGTCTACCA TTACTACAGC 
CGAATGGCCA ACTACAGGAA CACCAGTGAG ATCCCATTAG TGCTGGTGGG GACCCAGGAT 
GCCATAAGTT CCACCAACCC GAGGGTCATC GATGACGTCA GGGCTCGGAA GCTCTCCAAT 
GACCTGAAGA GGTGCACGTA CTATGAGACG TGCGCCACCT ATGGGCTCAA CGTGGAGCGG 
GTCTTCCAGG ACGTTGCCCA GAAGATCGTT GCCACAAGGA AGAAGCAGCA GCTATCCATA 
GGCCCCTGCA AGTCCTTACC CAACTCTCCA AGCCACTCGT CTGTGTGTTC TGCCCAAGTG 
TCCGCGGTAC ACATCAGCCA GACAAGTAAC GGAGGTGGGA GTTTAAGTGA CTACTCATCC 
TCTGTTCCTT CGACCCCAAG CACCAGCCAG AAAGAGCTTC GGATCGACGT CCCTCCCACT 
GCCAACACTC CAACGCCTGT CCGGAAGCAG TCCAAGCGCA GGTCCAACCT ATTCACATCT 
AGGAAAGGGA GCGACCCAGA CAAAGAGAAG AAAGGCCTGG AGAGCCGTGC GGACAGCATC 
GGGAGCGGGC GAGCCATCCC AATTAAACAG GGCATGCTGC TGAAGCGAAG TGGCAAGTCA 
CTGAACAAAG AGTGGAAAAA GAAGTACGTC ACCCTGTGCG ACAACGGTGT GCTGACCTAC 
CACCCCAGTT TACATGATTA CATGCAGAAT GTCCACGGCA AAGAGATAGA CCTCTTGAGG 
ACCACTGTGA AGGTGCCGGG GAAGAGGCCG CCCCGAGCCA CGTCTGCCTG TGCACCGATC 
TCCAGTCCCA AAACCAACGG CCTGGCCAAG GACATGAGCA GTTTACACAT CTCACCAAAT 
TCAGGGAATG TCACTAGTGC GTCTGGGTCT CAGATGGCAA GCGGCATCAG CCTGGTCTCC 
TTCAACAGCC GACCCGATGG CATGCACCAG CGCTCCTACT CAGTCTCCAG TGCCGACCAG 
TGGAGTGACG CTACAGTCAT TGCAAACTCA GCCATCAGCA GTGACACGGG GCTGGGTGAC 
TCGGTGTGTT CCAGCCCAAG TATCTCCAGC AGCACCAGCC CCAAGCTCGA CCCACCCCCC 
TCCCCCCACG CCAACAGAAA GAAGCACCGG AGGAAGAAAA GTACCAGCAA CTTCAAGGCT 
GACGGGCTCT CAGGCACTGC TGAAGAGCAA GAAGAAAACT TGGAGTTTAT CATTGTGTCC 
CTCACTGGCC AGACGTGGCA CTTTGAAGCC ACCACATACG AGGAGCGAGA CGCATGGGTC 
CAAGCCATCG AGAGCCAGAT CCTCGCCAGC CTGCAGTCGT GCGAGAGCAG CAAGAATAAG 
TCCCGACTAA CCAGCCAGAG CGAAGCCATG GCCCTGCAGT CCATCCGTAA CATGAGAGGG 
AACTCGCACT GCGTGGACTG TGACACCCAA AACCCTAACT GGGCCAGTTT GAACTTGGGA 
GCCCTCATGT GTATCGAGTG CTCGGGGATC CACCGGAATC TCGGCACCCA CCTCTCTCGG 
GTCAGATCGC TAGACCTGGA CGACTGGCCC ATGGAGCTGA TCAAGGTGAT GTCATCCATC 
GGGAATGAGC TGGCCAACAG CGTGTGGGAA GAGGGCAGCC AGGGACGGAC AAAGCCCTCA 
CTGGACTCCA CCAGGGAAGA AAAGGAAAGG TGGATCCGGG CCAAGTATGA GCAGAAACTC 
TTCCTGGCCC CACTGCCATG CACAGAGTTC TCCCTGGGCC AGCAGCTGCT GCGTGCCACT 
GCCGAGGAAG ACCTGCGGAC TGTCATCCTA CTTCTGGCAC ATGGGTCCCG GGACGAGGTG 
AATGAGACCT GTGGGGAAGG AGACGGTCGC ACGGCGCTGC ACCTGGCCTG TCGCAAGGGC 
AACGTGGTCC TGGCTCAGCT GCTGATCTGG TACGGAGTGG ACGTCATGGC CCGAGACGCC 
CACGGGAACA CAGCACTGGC CTATGCCCGG CAGGCCTCCA GCCAGGAGTG TATTGACGTG 
CTTCTACAGT ATGGCTGCCC CGACGAGCGC TTTGTGCTCA TGGCTACCCC TAACCTGTCC 
AGGAAGAGCA ACAGCAGGAA CAACAGTAGC GGGAGGGCGC CCAGTGTCAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_835220.1
CDS	620..3193
Translation	MNYQQQLANSAAIRAEIQRFESVHPNIYSIYELLERVEEPVLQNQIREHVIAIEDAFVNSQEWTLSRSVPELKVGIVGNLASGKSALVHRYLTGTYVQEESPEGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFAMWVDAVIFVFSLEDEISFQTVYHYYSRMANYRNTSEIPLVLVGTQDAISSTNPRVIDDVRARKLSNDLKRCTYYETCATYGLNVERVFQDVAQKIVATRKKQQLSIGPCKSLPNSPSHSSVCSAQVSAVHISQTSNGGGSLSDYSSSVPSTPSTSQKELRIDVPPTANTPTPVRKQSKRRSNLFTSRKGSDPDKEKKGLESRADSIGSGRAIPIKQGMLLKRSGKSLNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRATSACAPISSPKTNGLAKDMSSLHISPNSGNVTSASGSQMASGISLVSFNSRPDGMHQRSYSVSSADQWSDATVIANSAISSDTGLGDSVCSSPSISSSTSPKLDPPPSPHANRKKHRRKKSTSNFKADGLSGTAEEQEENLEFIIVSLTGQTWHFEATTYEERDAWVQAIESQILASLQSCESSKNKSRLTSQSEAMALQSIRNMRGNSHCVDCDTQNPNWASLNLGALMCIECSGIHRNLGTHLSRVRSLDLDDWPMELIKVMSSIGNELANSVWEEGSQGRTKPSLDSTREEKERWIRAKYEQKLFLAPLPCTEFSLGQQLLRATAEEDLRTVILLLAHGSRDEVNETCGEGDGRTALHLACRKGNVVLAQLLIWYGVDVMARDAHGNTALAYARQASSQECIDVLLQYGCPDERFVLMATPNLSRKSNSRNNSSGRAPSVI

Target ORF information:

RefSeq Version	NM_178119.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178119.3

ORF Insert Sequence:

ATGAACTACC AGCAGCAGCT GGCCAACTCG GCTGCCATCC GGGCCGAGAT CCAGCGCTTC 
GAGTCGGTCC ACCCCAACAT CTACTCCATC TACGAGCTGC TGGAGCGCGT GGAGGAGCCG 
GTGCTGCAGA ACCAGATTCG GGAGCACGTC ATCGCCATCG AAGATGCTTT CGTGAACAGC 
CAGGAATGGA CACTCAGCCG ATCGGTACCG GAGCTCAAAG TGGGAATTGT GGGTAACCTG 
GCCAGTGGCA AGTCTGCGCT GGTGCACCGG TACCTGACAG GCACGTACGT GCAGGAGGAA 
TCTCCAGAAG GTGGCAGGTT CAAGAAGGAG ATTGTTGTTG ATGGACAGAG TTATCTGCTG 
CTGATTAGGG ATGAAGGGGG CCCCCCGGAG GCACAGTTCG CCATGTGGGT GGACGCGGTC 
ATCTTTGTCT TCAGCTTGGA GGATGAGATC AGTTTCCAGA CTGTCTACCA TTACTACAGC 
CGAATGGCCA ACTACAGGAA CACCAGTGAG ATCCCATTAG TGCTGGTGGG GACCCAGGAT 
GCCATAAGTT CCACCAACCC GAGGGTCATC GATGACGTCA GGGCTCGGAA GCTCTCCAAT 
GACCTGAAGA GGTGCACGTA CTATGAGACG TGCGCCACCT ATGGGCTCAA CGTGGAGCGG 
GTCTTCCAGG ACGTTGCCCA GAAGATCGTT GCCACAAGGA AGAAGCAGCA GCTATCCATA 
GGCCCCTGCA AGTCCTTACC CAACTCTCCA AGCCACTCGT CTGTGTGTTC TGCCCAAGTG 
TCCGCGGTAC ACATCAGCCA GACAAGTAAC GGAGGTGGGA GTTTAAGTGA CTACTCATCC 
TCTGTTCCTT CGACCCCAAG CACCAGCCAG AAAGAGCTTC GGATCGACGT CCCTCCCACT 
GCCAACACTC CAACGCCTGT CCGGAAGCAG TCCAAGCGCA GGTCCAACCT ATTCACATCT 
AGGAAAGGGA GCGACCCAGA CAAAGAGAAG AAAGGCCTGG AGAGCCGTGC GGACAGCATC 
GGGAGCGGGC GAGCCATCCC AATTAAACAG GGCATGCTGC TGAAGCGAAG TGGCAAGTCA 
CTGAACAAAG AGTGGAAAAA GAAGTACGTC ACCCTGTGCG ACAACGGTGT GCTGACCTAC 
CACCCCAGTT TACATGATTA CATGCAGAAT GTCCACGGCA AAGAGATAGA CCTCTTGAGG 
ACCACTGTGA AGGTGCCGGG GAAGAGGCCG CCCCGAGCCA CGTCTGCCTG TGCACCGATC 
TCCAGTCCCA AAACCAACGG CCTGGCCAAG GACATGAGCA GTTTACACAT CTCACCAAAT 
TCAGGGAATG TCACTAGTGC GTCTGGGTCT CAGATGGCAA GCGGCATCAG CCTGGTCTCC 
TTCAACAGCC GACCCGATGG CATGCACCAG CGCTCCTACT CAGTCTCCAG TGCCGACCAG 
TGGAGTGACG CTACAGTCAT TGCAAACTCA GCCATCAGCA GTGACACGGG GCTGGGTGAC 
TCGGTGTGTT CCAGCCCAAG TATCTCCAGC AGCACCAGCC CCAAGCTCGA CCCACCCCCC 
TCCCCCCACG CCAACAGAAA GAAGCACCGG AGGAAGAAAA GTACCAGCAA CTTCAAGGCT 
GACGGGCTCT CAGGCACTGC TGAAGAGCAA GAAGAAAACT TGGAGTTTAT CATTGTGTCC 
CTCACTGGCC AGACGTGGCA CTTTGAAGCC ACCACATACG AGGAGCGAGA CGCATGGGTC 
CAAGCCATCG AGAGCCAGAT CCTCGCCAGC CTGCAGTCGT GCGAGAGCAG CAAGAATAAG 
TCCCGACTAA CCAGCCAGAG CGAAGCCATG GCCCTGCAGT CCATCCGTAA CATGAGAGGG 
AACTCGCACT GCGTGGACTG TGACACCCAA AACCCTAACT GGGCCAGTTT GAACTTGGGA 
GCCCTCATGT GTATCGAGTG CTCGGGGATC CACCGGAATC TCGGCACCCA CCTCTCTCGG 
GTCAGATCGC TAGACCTGGA CGACTGGCCC ATGGAGCTGA TCAAGGTGAT GTCATCCATC 
GGGAATGAGC TGGCCAACAG CGTGTGGGAA GAGGGCAGCC AGGGACGGAC AAAGCCCTCA 
CTGGACTCCA CCAGGGAAGA AAAGGAAAGG TGGATCCGGG CCAAGTATGA GCAGAAACTC 
TTCCTGGCCC CACTGCCATG CACAGAGTTC TCCCTGGGCC AGCAGCTGCT GCGTGCCACT 
GCCGAGGAAG ACCTGCGGAC TGTCATCCTA CTTCTGGCAC ATGGGTCCCG GGACGAGGTG 
AATGAGACCT GTGGGGAAGG AGACGGTCGC ACGGCGCTGC ACCTGGCCTG TCGCAAGGGC 
AACGTGGTCC TGGCTCAGCT GCTGATCTGG TACGGAGTGG ACGTCATGGC CCGAGACGCC 
CACGGGAACA CAGCACTGGC CTATGCCCGG CAGGCCTCCA GCCAGGAGTG TATTGACGTG 
CTTCTACAGT ATGGCTGCCC CGACGAGCGC TTTGTGCTCA TGGCTACCCC TAACCTGTCC 
AGGAAGAGCA ACAGCAGGAA CAACAGTAGC GGGAGGGCGC CCAGTGTCAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agap1 ( NM_001037136.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001032213.1 Mus musculus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu12816
Clone ID Related Accession (Same CDS sequence)	NM_001037136.1
Accession Version	NM_001037136.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2415bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Mus musculus(house mouse)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147503.1, AK046523.1 and AC124227.21. Transcript Variant: This variant (2) lacks an alternate in-frame segment, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC096588.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTACC AGCAGCAGCT GGCCAACTCG GCTGCCATCC GGGCCGAGAT CCAGCGCTTC 
GAGTCGGTCC ACCCCAACAT CTACTCCATC TACGAGCTGC TGGAGCGCGT GGAGGAGCCG 
GTGCTGCAGA ACCAGATTCG GGAGCACGTC ATCGCCATCG AAGATGCTTT CGTGAACAGC 
CAGGAATGGA CACTCAGCCG ATCGGTACCG GAGCTCAAAG TGGGAATTGT GGGTAACCTG 
GCCAGTGGCA AGTCTGCGCT GGTGCACCGG TACCTGACAG GCACGTACGT GCAGGAGGAA 
TCTCCAGAAG GTGGCAGGTT CAAGAAGGAG ATTGTTGTTG ATGGACAGAG TTATCTGCTG 
CTGATTAGGG ATGAAGGGGG CCCCCCGGAG GCACAGTTCG CCATGTGGGT GGACGCGGTC 
ATCTTTGTCT TCAGCTTGGA GGATGAGATC AGTTTCCAGA CTGTCTACCA TTACTACAGC 
CGAATGGCCA ACTACAGGAA CACCAGTGAG ATCCCATTAG TGCTGGTGGG GACCCAGGAT 
GCCATAAGTT CCACCAACCC GAGGGTCATC GATGACGTCA GGGCTCGGAA GCTCTCCAAT 
GACCTGAAGA GGTGCACGTA CTATGAGACG TGCGCCACCT ATGGGCTCAA CGTGGAGCGG 
GTCTTCCAGG ACGTTGCCCA GAAGATCGTT GCCACAAGGA AGAAGCAGCA GCTATCCATA 
GGCCCCTGCA AGTCCTTACC CAACTCTCCA AGCCACTCGT CTGTGTGTTC TGCCCAAGTG 
TCCGCGGTAC ACATCAGCCA GACAAGTAAC GGAGGTGGGA GTTTAAGTGA CTACTCATCC 
TCTGTTCCTT CGACCCCAAG CACCAGCCAG AAAGAGCTTC GGATCGACGT CCCTCCCACT 
GCCAACACTC CAACGCCTGT CCGGAAGCAG TCCAAGCGCA GGTCCAACCT ATTCACATCT 
AGGAAAGGGA GCGACCCAGA CAAAGAGAAG AAAGGCCTGG AGAGCCGTGC GGACAGCATC 
GGGAGCGGGC GAGCCATCCC AATTAAACAG GGCATGCTGC TGAAGCGAAG TGGCAAGTCA 
CTGAACAAAG AGTGGAAAAA GAAGTACGTC ACCCTGTGCG ACAACGGTGT GCTGACCTAC 
CACCCCAGTT TACATGATTA CATGCAGAAT GTCCACGGCA AAGAGATAGA CCTCTTGAGG 
ACCACTGTGA AGGTGCCGGG GAAGAGGCCG CCCCGAGCCA CGTCTGCCTG TGCACCGATC 
TCCAGTCCCA AAACCAACGG CCTGGCCAAG GACATGAGCA GTTTACACAT CTCACCAAAT 
TCAGACACGG GGCTGGGTGA CTCGGTGTGT TCCAGCCCAA GTATCTCCAG CAGCACCAGC 
CCCAAGCTCG ACCCACCCCC CTCCCCCCAC GCCAACAGAA AGAAGCACCG GAGGAAGAAA 
AGTACCAGCA ACTTCAAGGC TGACGGGCTC TCAGGCACTG CTGAAGAGCA AGAAGAAAAC 
TTGGAGTTTA TCATTGTGTC CCTCACTGGC CAGACGTGGC ACTTTGAAGC CACCACATAC 
GAGGAGCGAG ACGCATGGGT CCAAGCCATC GAGAGCCAGA TCCTCGCCAG CCTGCAGTCG 
TGCGAGAGCA GCAAGAATAA GTCCCGACTA ACCAGCCAGA GCGAAGCCAT GGCCCTGCAG 
TCCATCCGTA ACATGAGAGG GAACTCGCAC TGCGTGGACT GTGACACCCA AAACCCTAAC 
TGGGCCAGTT TGAACTTGGG AGCCCTCATG TGTATCGAGT GCTCGGGGAT CCACCGGAAT 
CTCGGCACCC ACCTCTCTCG GGTCAGATCG CTAGACCTGG ACGACTGGCC CATGGAGCTG 
ATCAAGGTGA TGTCATCCAT CGGGAATGAG CTGGCCAACA GCGTGTGGGA AGAGGGCAGC 
CAGGGACGGA CAAAGCCCTC ACTGGACTCC ACCAGGGAAG AAAAGGAAAG GTGGATCCGG 
GCCAAGTATG AGCAGAAACT CTTCCTGGCC CCACTGCCAT GCACAGAGTT CTCCCTGGGC 
CAGCAGCTGC TGCGTGCCAC TGCCGAGGAA GACCTGCGGA CTGTCATCCT ACTTCTGGCA 
CATGGGTCCC GGGACGAGGT GAATGAGACC TGTGGGGAAG GAGACGGTCG CACGGCGCTG 
CACCTGGCCT GTCGCAAGGG CAACGTGGTC CTGGCTCAGC TGCTGATCTG GTACGGAGTG 
GACGTCATGG CCCGAGACGC CCACGGGAAC ACAGCACTGG CCTATGCCCG GCAGGCCTCC 
AGCCAGGAGT GTATTGACGT GCTTCTACAG TATGGCTGCC CCGACGAGCG CTTTGTGCTC 
ATGGCTACCC CTAACCTGTC CAGGAAGAGC AACAGCAGGA ACAACAGTAG CGGGAGGGCG 
CCCAGTGTCA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032213.1
CDS	620..3034
Translation	MNYQQQLANSAAIRAEIQRFESVHPNIYSIYELLERVEEPVLQNQIREHVIAIEDAFVNSQEWTLSRSVPELKVGIVGNLASGKSALVHRYLTGTYVQEESPEGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFAMWVDAVIFVFSLEDEISFQTVYHYYSRMANYRNTSEIPLVLVGTQDAISSTNPRVIDDVRARKLSNDLKRCTYYETCATYGLNVERVFQDVAQKIVATRKKQQLSIGPCKSLPNSPSHSSVCSAQVSAVHISQTSNGGGSLSDYSSSVPSTPSTSQKELRIDVPPTANTPTPVRKQSKRRSNLFTSRKGSDPDKEKKGLESRADSIGSGRAIPIKQGMLLKRSGKSLNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRATSACAPISSPKTNGLAKDMSSLHISPNSDTGLGDSVCSSPSISSSTSPKLDPPPSPHANRKKHRRKKSTSNFKADGLSGTAEEQEENLEFIIVSLTGQTWHFEATTYEERDAWVQAIESQILASLQSCESSKNKSRLTSQSEAMALQSIRNMRGNSHCVDCDTQNPNWASLNLGALMCIECSGIHRNLGTHLSRVRSLDLDDWPMELIKVMSSIGNELANSVWEEGSQGRTKPSLDSTREEKERWIRAKYEQKLFLAPLPCTEFSLGQQLLRATAEEDLRTVILLLAHGSRDEVNETCGEGDGRTALHLACRKGNVVLAQLLIWYGVDVMARDAHGNTALAYARQASSQECIDVLLQYGCPDERFVLMATPNLSRKSNSRNNSSGRAPSVI

Target ORF information:

RefSeq Version	NM_001037136.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037136.1

ORF Insert Sequence:

ATGAACTACC AGCAGCAGCT GGCCAACTCG GCTGCCATCC GGGCCGAGAT CCAGCGCTTC 
GAGTCGGTCC ACCCCAACAT CTACTCCATC TACGAGCTGC TGGAGCGCGT GGAGGAGCCG 
GTGCTGCAGA ACCAGATTCG GGAGCACGTC ATCGCCATCG AAGATGCTTT CGTGAACAGC 
CAGGAATGGA CACTCAGCCG ATCGGTACCG GAGCTCAAAG TGGGAATTGT GGGTAACCTG 
GCCAGTGGCA AGTCTGCGCT GGTGCACCGG TACCTGACAG GCACGTACGT GCAGGAGGAA 
TCTCCAGAAG GTGGCAGGTT CAAGAAGGAG ATTGTTGTTG ATGGACAGAG TTATCTGCTG 
CTGATTAGGG ATGAAGGGGG CCCCCCGGAG GCACAGTTCG CCATGTGGGT GGACGCGGTC 
ATCTTTGTCT TCAGCTTGGA GGATGAGATC AGTTTCCAGA CTGTCTACCA TTACTACAGC 
CGAATGGCCA ACTACAGGAA CACCAGTGAG ATCCCATTAG TGCTGGTGGG GACCCAGGAT 
GCCATAAGTT CCACCAACCC GAGGGTCATC GATGACGTCA GGGCTCGGAA GCTCTCCAAT 
GACCTGAAGA GGTGCACGTA CTATGAGACG TGCGCCACCT ATGGGCTCAA CGTGGAGCGG 
GTCTTCCAGG ACGTTGCCCA GAAGATCGTT GCCACAAGGA AGAAGCAGCA GCTATCCATA 
GGCCCCTGCA AGTCCTTACC CAACTCTCCA AGCCACTCGT CTGTGTGTTC TGCCCAAGTG 
TCCGCGGTAC ACATCAGCCA GACAAGTAAC GGAGGTGGGA GTTTAAGTGA CTACTCATCC 
TCTGTTCCTT CGACCCCAAG CACCAGCCAG AAAGAGCTTC GGATCGACGT CCCTCCCACT 
GCCAACACTC CAACGCCTGT CCGGAAGCAG TCCAAGCGCA GGTCCAACCT ATTCACATCT 
AGGAAAGGGA GCGACCCAGA CAAAGAGAAG AAAGGCCTGG AGAGCCGTGC GGACAGCATC 
GGGAGCGGGC GAGCCATCCC AATTAAACAG GGCATGCTGC TGAAGCGAAG TGGCAAGTCA 
CTGAACAAAG AGTGGAAAAA GAAGTACGTC ACCCTGTGCG ACAACGGTGT GCTGACCTAC 
CACCCCAGTT TACATGATTA CATGCAGAAT GTCCACGGCA AAGAGATAGA CCTCTTGAGG 
ACCACTGTGA AGGTGCCGGG GAAGAGGCCG CCCCGAGCCA CGTCTGCCTG TGCACCGATC 
TCCAGTCCCA AAACCAACGG CCTGGCCAAG GACATGAGCA GTTTACACAT CTCACCAAAT 
TCAGACACGG GGCTGGGTGA CTCGGTGTGT TCCAGCCCAA GTATCTCCAG CAGCACCAGC 
CCCAAGCTCG ACCCACCCCC CTCCCCCCAC GCCAACAGAA AGAAGCACCG GAGGAAGAAA 
AGTACCAGCA ACTTCAAGGC TGACGGGCTC TCAGGCACTG CTGAAGAGCA AGAAGAAAAC 
TTGGAGTTTA TCATTGTGTC CCTCACTGGC CAGACGTGGC ACTTTGAAGC CACCACATAC 
GAGGAGCGAG ACGCATGGGT CCAAGCCATC GAGAGCCAGA TCCTCGCCAG CCTGCAGTCG 
TGCGAGAGCA GCAAGAATAA GTCCCGACTA ACCAGCCAGA GCGAAGCCAT GGCCCTGCAG 
TCCATCCGTA ACATGAGAGG GAACTCGCAC TGCGTGGACT GTGACACCCA AAACCCTAAC 
TGGGCCAGTT TGAACTTGGG AGCCCTCATG TGTATCGAGT GCTCGGGGAT CCACCGGAAT 
CTCGGCACCC ACCTCTCTCG GGTCAGATCG CTAGACCTGG ACGACTGGCC CATGGAGCTG 
ATCAAGGTGA TGTCATCCAT CGGGAATGAG CTGGCCAACA GCGTGTGGGA AGAGGGCAGC 
CAGGGACGGA CAAAGCCCTC ACTGGACTCC ACCAGGGAAG AAAAGGAAAG GTGGATCCGG 
GCCAAGTATG AGCAGAAACT CTTCCTGGCC CCACTGCCAT GCACAGAGTT CTCCCTGGGC 
CAGCAGCTGC TGCGTGCCAC TGCCGAGGAA GACCTGCGGA CTGTCATCCT ACTTCTGGCA 
CATGGGTCCC GGGACGAGGT GAATGAGACC TGTGGGGAAG GAGACGGTCG CACGGCGCTG 
CACCTGGCCT GTCGCAAGGG CAACGTGGTC CTGGCTCAGC TGCTGATCTG GTACGGAGTG 
GACGTCATGG CCCGAGACGC CCACGGGAAC ACAGCACTGG CCTATGCCCG GCAGGCCTCC 
AGCCAGGAGT GTATTGACGT GCTTCTACAG TATGGCTGCC CCGACGAGCG CTTTGTGCTC 
ATGGCTACCC CTAACCTGTC CAGGAAGAGC AACAGCAGGA ACAACAGTAG CGGGAGGGCG 
CCCAGTGTCA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Endosome Localized Arf-GAP AGAP1 Modulates Dendritic Spine Morphology Downstream of the Neurodevelopmental Disorder Factor Dysbindin.
Frontiers in cellular neuroscience10218(2016)
Arnold M,Cross R,Singleton KS,Zlatic S,Chapleau C,Mullin AP,Rolle I,Moore CC,Theibert A,Pozzo-Miller L,Faundez V,Larimore J

GTP-binding protein-like domain of AGAP1 is protein binding site that allosterically regulates ArfGAP protein catalytic activity.
The Journal of biological chemistry287(21)17176-85(2012 May)
Luo R,Akpan IO,Hayashi R,Sramko M,Barr V,Shiba Y,Randazzo PA

AGAP1/AP-3-dependent endocytic recycling of M5 muscarinic receptors promotes dopamine release.
The EMBO journal29(16)2813-26(2010 Aug)
Bendor J,Lizardi-Ortiz JE,Westphalen RI,Brandstetter M,Hemmings HC Jr,Sulzer D,Flajolet M,Greengard P

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Development, growth & differentiation47(3)163-72(2005 Apr)
Taniwaki T,Haruna K,Nakamura H,Sekimoto T,Oike Y,Imaizumi T,Saito F,Muta M,Soejima Y,Utoh A,Nakagata N,Araki M,Yamamura K,Araki K

AGAP1, a novel binding partner of nitric oxide-sensitive guanylyl cyclase.
The Journal of biological chemistry279(47)49346-54(2004 Nov)
Meurer S,Pioch S,Wagner K,M?ller-Esterl W,Gross S

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Agap1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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