gRNAs
The following Thoc2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Thoc2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu56041 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006541503.3 , XM_006541503.4 | |
| Accession Version | XM_006541503.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4866bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | THO complex subunit 2 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006541503.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCGGCCG CGGCTGTGGT GGTTCCCGCA GAGTGGATAA AGAACTGGGA GAAATCAGGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006541566.1 |
| CDS | 67..4932 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_006541503.4 |
1 | ATGGCGGCCG CGGCTGTGGT GGTTCCCGCA GAGTGGATAA AGAACTGGGA GAAATCAGGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu20859 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001033422.1 | |
| Accession Version | NM_001033422.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4785bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-09-09 | |
| Organism | Mus musculus(house mouse) | |
| Product | THO complex subunit 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK138023.1, AK140950.1, AK051141.1, CN537042.1, CO045267.1, AK031896.1, CX213978.1, CX203600.1 and CF423635.1. On or before Feb 14, 2007 this sequence version replaced XM_284697.6, XM_886363.2, XM_359343.4, XM_982464.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGCGGCCG CGGCTGTGGT GGTTCCCGCA GAGTGGATAA AGAACTGGGA GAAATCAGGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001028594.1 |
| CDS | 31..4815 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001033422.1 |
1 | ATGGCGGCCG CGGCTGTGGT GGTTCCCGCA GAGTGGATAA AGAACTGGGA GAAATCAGGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu56041 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006541503.3 , XM_006541503.4 | |
| Accession Version | XM_006541503.3 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4866bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-21 | |
| Organism | Mus musculus(house mouse) | |
| Product | THO complex subunit 2 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006541503.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCGGCCG CGGCTGTGGT GGTTCCCGCA GAGTGGATAA AGAACTGGGA GAAATCAGGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006541566.1 |
| CDS | 49..4914 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006541503.3 |
1 | ATGGCGGCCG CGGCTGTGGT GGTTCCCGCA GAGTGGATAA AGAACTGGGA GAAATCAGGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
The THO complex regulates pluripotency gene mRNA export and controls embryonic stem cell self-renewal and somatic cell reprogramming. |
Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method. |
