Cacna2d4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cacna2d4
Entrez Gene ID	319734
Full Name	calcium channel, voltage-dependent, alpha 2/delta subunit 4
Synonyms	5730412N02Rik,BE686333
General protein information	Preferred Names calcium channel, voltage-dependent, alpha 2/delta subunit 4 Names voltage-dependent calcium channel subunit alpha-2/delta-4 voltage-gated calcium channel subunit alpha-2/delta-4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001033382.3	NP_001028554.3	voltage-dependent calcium channel subunit alpha-2/delta-4 isoform 1 precursor
NM_001347427.2	NP_001334356.2	voltage-dependent calcium channel subunit alpha-2/delta-4 isoform 2
NM_001362214.1	NP_001349143.1	voltage-dependent calcium channel subunit alpha-2/delta-4 isoform 3
NM_001033382.2	NP_001028554.2	voltage-dependent calcium channel subunit alpha-2/delta-4 isoform 1
NM_001347427.1	NP_001334356.1	voltage-dependent calcium channel subunit alpha-2/delta-4 isoform 2
XM_030255452.1	XP_030111312.1	voltage-dependent calcium channel subunit alpha-2/delta-4 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Cacna2d4	NP_001028554.2
Danio rerio (zebrafish)	cacna2d4a	XP_696635.4
Drosophila melanogaster (fruit fly)	CG4587	NP_001260467.1
Xenopus tropicalis (tropical clawed frog)	cacna2d4	XP_002938150.2
Homo sapiens (human)	CACNA2D4	NP_758952.4
Canis lupus familiaris (dog)	CACNA2D4	XP_534930.2
Bos taurus (cattle)	CACNA2D4	XP_002707867.2
Danio rerio (zebrafish)	cacna2d4b	XP_001920592.4
Pan troglodytes (chimpanzee)	CACNA2D4	XP_001152728.2
Rattus norvegicus (Norway rat)	Cacna2d4	NP_001178680.1
Gallus gallus (chicken)	CACNA2D4	XP_003640465.2
	CACNA2D4	XP_001117901.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009598	XP_318625.3

Gene Ontology
Bibliography

Related articles in PubMed

The Auxiliary Calcium Channel Subunit ?2?4 Is Required for Axonal Elaboration, Synaptic Transmission, and Wiring of Rod Photoreceptors.
Wang Y, Fehlhaber KE, Sarria I, Cao Y, Ingram NT, Guerrero-Given D, Throesch B, Baldwin K, Kamasawa N, Ohtsuka T, Sampath AP, Martemyanov KA
Neuron93(6)1359-1374.e6(2017 Mar)

A New Splicing Isoform of Cacna2d4 Mimicking the Effects of c.2451insC Mutation in the Retina: Novel Molecular and Electrophysiological Insights.
Bacchi N, Messina A, Burtscher V, Dassi E, Provenzano G, Bozzi Y, Demontis GC, Koschak A, Denti MA, Casarosa S
Investigative ophthalmology & visual science56(8)4846-56(2015 Jul)

Differential neuronal targeting of a new and two known calcium channel ?4 subunit splice variants correlates with their regulation of gene expression.
Etemad S, Obermair GJ, Bindreither D, Benedetti A, Stanika R, Di Biase V, Burtscher V, Koschak A, Kofler R, Geley S, Wille A, Lusser A, Flockerzi V, Flucher BE
The Journal of neuroscience : the official journal of the Society for Neuroscience34(4)1446-61(2014 Jan)

Voltage-activated calcium channel expression profiles in mouse brain and cultured hippocampal neurons.
Schlick B, Flucher BE, Obermair GJ
Neuroscience167(3)786-98(2010 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Eliminating a2d4 in mice abolishes rod synaptogenesis and synaptic transmission to rod ON bipolar cells, and disrupts postsynaptic mGluR6 clustering. Further find , a2d4 is crucial for organizing synaptic ribbons and setting CaV1.4 voltage sensitivity. In cones, a2d4 is essential for CaV1.4 function, but is not required for ribbon organization, synaptogenesis, or synaptic transmission.
Title: The Auxiliary Calcium Channel Subunit ?2?4 Is Required for Axonal Elaboration, Synaptic Transmission, and Wiring of Rod Photoreceptors.

alpha2delta4 E25b splicing variant is normally present in mouse retina and mimics the effect of c.2451insC mutation.
Title: A New Splicing Isoform of Cacna2d4 Mimicking the Effects of c.2451insC Mutation in the Retina: Novel Molecular and Electrophysiological Insights.

The Cacna2d4 mutation underlies a novel channelopathy leading to cone-rod dysfunction in the visual system of mice
Title: Structural and functional abnormalities of retinal ribbon synapses due to Cacna2d4 mutation.

The following Cacna2d4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cacna2d4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu84278	NM_001362214.1 Latest version!	Mus musculus calcium channel, voltage-dependent, alpha 2/delta subunit 4 (Cacna2d4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu89225	XM_030255452.1 Latest version!	Mus musculus calcium channel, voltage-dependent, alpha 2/delta subunit 4 (Cacna2d4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu84763	NM_001347427.2 Latest version!	Mus musculus calcium channel, voltage-dependent, alpha 2/delta subunit 4 (Cacna2d4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu84765	NM_001033382.3 Latest version!	Mus musculus calcium channel, voltage-dependent, alpha 2/delta subunit 4 (Cacna2d4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu12003	NM_001033382.2 Latest version!	Mus musculus calcium channel, voltage-dependent, alpha 2/delta subunit 4 (Cacna2d4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$671.30 $959.00
OMu37354	NM_001347427.1 Latest version!	Mus musculus calcium channel, voltage-dependent, alpha 2/delta subunit 4 (Cacna2d4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cacna2d4 ( NM_001362214.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001349143.1 Mus musculus calcium channel, voltage-dependent, alpha 2/delta subunit 4 (Cacna2d4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu84278
Clone ID Related Accession (Same CDS sequence)	NM_001362214.1
Accession Version	NM_001362214.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Mus musculus(house mouse)
Product	voltage-dependent calcium channel subunit alpha-2/delta-4 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC115816.9. Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.56390.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAAGCAG ACTACCTCCA GCGCCAGTTC TGGGCAGCCA TGCAGCAGTG CAACGCTGTA 
GAGGGGCCGT GCCTGAAGAG CTGCGAGGAC ACTGATCTCG ACTGCTTCGT CATAGACAAC 
AATGGCTTCG TCCTGATCTC AGAGAGACCC CAAGAGATGG GAAGACTTCT GGGGGAAGCG 
GATGGTGCTC TCATGAAGCA ACTTCTCAGC ATGGGGGTGT TCAGCCGTGT GACCATGTAT 
GACTACCAGG CCATGTGCAA GCCCCCAGAT CATCACCACA GTGCGGCCGA ATCCCTGTTC 
AGTCCTCTCT CTGCCTTCTT GATGGTGGCC AGGTGGCTAC TGCATGAATG TCTCCTATTC 
CTGTTAGAGT GGAGTGCCTG GGGATCTTGG CAGGACAAAG GGTCAGAGGC CCACAAGCAC 
AAGAAGCAGG ACCTGCTGCA CCCCTGTGAC ACAGAGTACC CAGTGTTCGT GCATCAGACG 
GCCATCCAGG AAGCCAATGG CATCATCGAG TGTGGGGGCT GCCAGAAGAC ATTCGTGATG 
CAACAGATTC CCCGAAGCAA CCTGCTCCTG CTCGTGACAG ACCGCACCTG TGACTGCAGC 
GCCCACTCTC CCATCCTCCA GGAGGCCACA GAAGTCAAAT ATAATGCCTC TGTCAAGTGT 
GACAGGATGC GCTCCCAGAA GCCCCGGAGA CGACCAGGCT CCTGCCATGC CTTCCATCCA 
GAGGAGAATG CCCAGGACTG TGGCGGTGCT TCAGACACTC TGCCTTCATC CCCTCTGCTC 
CTGCTATCAC TGGGGGCCTG GCTGCTTCCA CCCCAGCTTC TGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349143.1
CDS	159..986
Translation	MQADYLQRQFWAAMQQCNAVEGPCLKSCEDTDLDCFVIDNNGFVLISERPQEMGRLLGEADGALMKQLLSMGVFSRVTMYDYQAMCKPPDHHHSAAESLFSPLSAFLMVARWLLHECLLFLLEWSAWGSWQDKGSEAHKHKKQDLLHPCDTEYPVFVHQTAIQEANGIIECGGCQKTFVMQQIPRSNLLLLVTDRTCDCSAHSPILQEATEVKYNASVKCDRMRSQKPRRRPGSCHAFHPEENAQDCGGASDTLPSSPLLLLSLGAWLLPPQLLW

Target ORF information:

RefSeq Version	NM_001362214.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, alpha 2/delta subunit 4 (Cacna2d4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362214.1

ORF Insert Sequence:

ATGCAAGCAG ACTACCTCCA GCGCCAGTTC TGGGCAGCCA TGCAGCAGTG CAACGCTGTA 
GAGGGGCCGT GCCTGAAGAG CTGCGAGGAC ACTGATCTCG ACTGCTTCGT CATAGACAAC 
AATGGCTTCG TCCTGATCTC AGAGAGACCC CAAGAGATGG GAAGACTTCT GGGGGAAGCG 
GATGGTGCTC TCATGAAGCA ACTTCTCAGC ATGGGGGTGT TCAGCCGTGT GACCATGTAT 
GACTACCAGG CCATGTGCAA GCCCCCAGAT CATCACCACA GTGCGGCCGA ATCCCTGTTC 
AGTCCTCTCT CTGCCTTCTT GATGGTGGCC AGGTGGCTAC TGCATGAATG TCTCCTATTC 
CTGTTAGAGT GGAGTGCCTG GGGATCTTGG CAGGACAAAG GGTCAGAGGC CCACAAGCAC 
AAGAAGCAGG ACCTGCTGCA CCCCTGTGAC ACAGAGTACC CAGTGTTCGT GCATCAGACG 
GCCATCCAGG AAGCCAATGG CATCATCGAG TGTGGGGGCT GCCAGAAGAC ATTCGTGATG 
CAACAGATTC CCCGAAGCAA CCTGCTCCTG CTCGTGACAG ACCGCACCTG TGACTGCAGC 
GCCCACTCTC CCATCCTCCA GGAGGCCACA GAAGTCAAAT ATAATGCCTC TGTCAAGTGT 
GACAGGATGC GCTCCCAGAA GCCCCGGAGA CGACCAGGCT CCTGCCATGC CTTCCATCCA 
GAGGAGAATG CCCAGGACTG TGGCGGTGCT TCAGACACTC TGCCTTCATC CCCTCTGCTC 
CTGCTATCAC TGGGGGCCTG GCTGCTTCCA CCCCAGCTTC TGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna2d4 ( XM_030255452.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030111312.1 Mus musculus calcium channel, voltage-dependent, alpha 2/delta subunit 4 (Cacna2d4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu89225
Clone ID Related Accession (Same CDS sequence)	XM_030255452.1
Accession Version	XM_030255452.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent calcium channel subunit alpha-2/delta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAC AGCTGGGACA GGAAGGACAA GGGCAGGGGA AGGACTCAGC TGTGGGCATC 
CAGATGCAAG CAGACTACCT CCAGCGCCAG TTCTGGGCAG CCATGCAGCA GTGCAACGCT 
GTAGAGGGGC CGTGCCTGAA GAGCTGCGAG GACACTGATC TCGACTGCTT CGTCATAGAC 
AACAATGGCT TCGTCCTGAT CTCAGAGAGA CCCCAAGAGA TGGGAAGACT TCTGGGGGAA 
GCGGATGGTG CTCTCATGAA GCAACTTCTC AGCATGGGGG TGTTCAGCCG TGTGACCATG 
TATGACTACC AGGCCATGTG CAAGCCCCCA GATCATCACC ACAGTGCGGC CGAATCCCTG 
TTCAGTCCTC TCTCTGCCTT CTTGATGGTG GCCAGGTGGC TACTGCATGA ATGTCTCCTA 
TTCCTGTTAG AGTGGAGTGC CTGGGGATCT TGGCAGGACA AAGGGTCAGA GGCCCACAAG 
CACAAGAAGC AGGACCTGCT GCACCCCTGT GACACAGAGT ACCCAGTGTT CGTGCATCAG 
ACGGCCATCC AGGAAGCCAA TGGCATCATC GAGTGTGGGG GCTGCCAGAA GACATTCGTG 
ATGCAACAGA TTCCCCGAAG CAACCTGCTC CTGCTCGTGA CAGACCGCAC CTGTGACTGC 
AGCGCCCACT CTCCCATCCT CCAGGAGGCC ACAGAAGTCA AATATAATGC CTCTGTCAAG 
TGTGACAGGA TGCGCTCCCA GAAGCCCCGG AGACGACCAG GCTCCTGCCA TGCCTTCCAT 
CCAGAGGAGA ATGCCCAGGA CTGTGGCGGT GCTTCAGACA CTCTGCCTTC ATCCCCTCTG 
CTCCTGCTAT CACTGGGGGC CTGGCTGCTT CCACCCCAGC TTCTGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030111312.1
CDS	215..1105
Translation	MGRQLGQEGQGQGKDSAVGIQMQADYLQRQFWAAMQQCNAVEGPCLKSCEDTDLDCFVIDNNGFVLISERPQEMGRLLGEADGALMKQLLSMGVFSRVTMYDYQAMCKPPDHHHSAAESLFSPLSAFLMVARWLLHECLLFLLEWSAWGSWQDKGSEAHKHKKQDLLHPCDTEYPVFVHQTAIQEANGIIECGGCQKTFVMQQIPRSNLLLLVTDRTCDCSAHSPILQEATEVKYNASVKCDRMRSQKPRRRPGSCHAFHPEENAQDCGGASDTLPSSPLLLLSLGAWLLPPQLLW

Target ORF information:

RefSeq Version	XM_030255452.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, alpha 2/delta subunit 4 (Cacna2d4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030255452.1

ORF Insert Sequence:

ATGGGAAGAC AGCTGGGACA GGAAGGACAA GGGCAGGGGA AGGACTCAGC TGTGGGCATC 
CAGATGCAAG CAGACTACCT CCAGCGCCAG TTCTGGGCAG CCATGCAGCA GTGCAACGCT 
GTAGAGGGGC CGTGCCTGAA GAGCTGCGAG GACACTGATC TCGACTGCTT CGTCATAGAC 
AACAATGGCT TCGTCCTGAT CTCAGAGAGA CCCCAAGAGA TGGGAAGACT TCTGGGGGAA 
GCGGATGGTG CTCTCATGAA GCAACTTCTC AGCATGGGGG TGTTCAGCCG TGTGACCATG 
TATGACTACC AGGCCATGTG CAAGCCCCCA GATCATCACC ACAGTGCGGC CGAATCCCTG 
TTCAGTCCTC TCTCTGCCTT CTTGATGGTG GCCAGGTGGC TACTGCATGA ATGTCTCCTA 
TTCCTGTTAG AGTGGAGTGC CTGGGGATCT TGGCAGGACA AAGGGTCAGA GGCCCACAAG 
CACAAGAAGC AGGACCTGCT GCACCCCTGT GACACAGAGT ACCCAGTGTT CGTGCATCAG 
ACGGCCATCC AGGAAGCCAA TGGCATCATC GAGTGTGGGG GCTGCCAGAA GACATTCGTG 
ATGCAACAGA TTCCCCGAAG CAACCTGCTC CTGCTCGTGA CAGACCGCAC CTGTGACTGC 
AGCGCCCACT CTCCCATCCT CCAGGAGGCC ACAGAAGTCA AATATAATGC CTCTGTCAAG 
TGTGACAGGA TGCGCTCCCA GAAGCCCCGG AGACGACCAG GCTCCTGCCA TGCCTTCCAT 
CCAGAGGAGA ATGCCCAGGA CTGTGGCGGT GCTTCAGACA CTCTGCCTTC ATCCCCTCTG 
CTCCTGCTAT CACTGGGGGC CTGGCTGCTT CCACCCCAGC TTCTGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna2d4 ( NM_001347427.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001334356.2 Mus musculus calcium channel, voltage-dependent, alpha 2/delta subunit 4 (Cacna2d4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu84763
Clone ID Related Accession (Same CDS sequence)	NM_001347427.2
Accession Version	NM_001347427.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Mus musculus(house mouse)
Product	voltage-dependent calcium channel subunit alpha-2/delta-4 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC115816.9. On May 2, 2019 this sequence version replaced NM_001347427.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes isoform 2 which is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC141091.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164136 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAAGAT GCCCCATGCT GTCCTCTAGC CACAACCAAG ACCACAGTGG ACGGTGGACA 
GCAGTGTGGA CCTCCCTGTG GAAGACGCCA ATCATCCTGT GGCTGCTGCT CTCGGACACC 
TCCCTGCCTA CCGTGAGGGG CCAGACCACA GTTCCTCTGG AAACAGTGAA GCTGTGGGCC 
GATACCTTCG GCAGGAATCT GTACAACACG GTGACCAGAT ATTCAGGCTC CCTCCTGCTG 
CAGAAGAAGT ACAAGGATGC AGAACCCAGT CTGAAGATCA AAGAGGTGGA TGGCTTGGAG 
CTGGTGAAGA AGTTCTCGGA GGACATGGAG ACCATGCTTC GGAGGAAAGT CGAGGCTGTT 
GAGAGCCTGG TGGAGGCCGC TGAGGAGGCT GACCTGAATC ATGAGTTCAA TGCATCTCTG 
GTGTTTAACT ACTACAACTC GGTACTAATC AATGAGAAGG ATGACAAGGG CAACTACGTG 
GAGCTGGGAG CTGAGTTTCT TCTCGAGTCC GACGCGCACT TCAGCAACCT GAGGGTGAAT 
GTCTCCATGA GCAGCGTTCA ACTGCCCACC AACGTGTACA ACAAAGACCC AGACATTTTA 
AATGGCGTGT ATATGTCCGA AGCCCTGAAC CCCGTGTTTG TGGAGAACTT TCAGAGAGAC 
CCTACATTGA CATGGCAGTA TTTCGGCAGC TCAACTGGGT TCTTCAGGAT CTATCCAGGA 
ATAAAGTGGA TGCCTGATGA AAACGGAGTC ATCGCCTTCG ACTGCAGAAA CCGTGGCTGG 
TACATACAAG CTGCCACATC TCCCAAAGAC ATCGTAATTC TGGTGGACAT TAGCGGCAGC 
ATGAAGGGGC TGAGGATGGC TATCGCCAAG CACACCATTA CCACCATATT GGACACCCTG 
GGAGAGAACG ACTTCGTGAA CATCATCGCG TACAATGACT ACGTCCACTA CATTGAGCCC 
TGCTTCAAAG GCATCCTTGT CCAAGCAGAT CGAGACAACC GAGAGCATTT CAAGCAGTTG 
GTGGATGAGC TGATGGTCAA AGGTGTGGGT GTCGTGAGTC AAGCTCTAAT TGAAGCTTTC 
GAGATCCTGA AACAGTTCCA AGAGTCCAAA CAAGGAAGTC TCTGCAACCA GGCCATCATG 
CTTATCACCG ATGGGGCTGT GGAAGACTAT GAGCCCGTGT TTGAGACCTA TAACTGGCCA 
GACCGAAAGG TCCGAGTTTT CACTTACCTT ATTGGAAGAG AAGTGACTTT CGCTGACCGC 
ATGAAGTGGA TCGCCTGCAA CAATAAAGGC TACTACACAC AGATCTCCAC ACTGGCGGAT 
GCGCAGGAGA GCGTGATGGA GTACCTGCAT GTGCTTAGCC GCCCCATGGT CATCAACCAT 
GACCACGACA TCATCTGGAC AGAGGCTTAC ATGGACAGCC GGCTCTTCAC ATCAGAGGCA 
CAGAGCCTGA TGCTCCTCAC CACAGTGGCC ATGCCTGTCT TCAGCAAAAA GAATGAAACA 
AGATCCCATG GCATTCTCCT GGGTGTAGTG GGCTCTGACG TGACCCTAAG AGAGCTCATG 
AAGCTGGCAC CCCGATATAA GTACAGAGAA GGCAAGAAGC TGAGACCCAA ACCCAACTAC 
AACAGTGTGG ACCTCTCAGA AGTGGAGTGG GAGGACCAAG CTGAAATTCT GAGGACCGCC 
ATGATCAATG GGGAAACCGG GTCTCACTCC ATGGACGTGA AGGTGCCACT GGACAAAGGG 
AAGCGAGTTC TATTCCTGAC CAATGACTAC TTCTTCACAG ACATCAGTGA CACACCTTTC 
AGCCTGGGAG TGGTGCTCAC CAGAGGTCAT GGAGAATACA TCCTCCTGGG GAACACATCT 
GTGGAAGAAG GCCTGCACGA CTTGCTTCAT CCGGATCTGA CCCTGGCCAG TGACTGGATC 
TACTGTATCA CAGATATCGA CCCGGACCAC CGGAAACTCA GCCAACTGGA AGCTGTGGTT 
CGTTTCCTGA CAGGGGTGGA TCCAGACCTG GAGTGTGACG AGGAACTGGT GCGGGAGGTG 
CTGTTTGATG CGGTGGTAAC CGCCCCCATG GAAGCCTATT GGACAGCACT GGCACTCAAC 
ATCTCTGAAG AGTCAGAGCC TGGCGTGGAT GTGGCCTTCC TGGGAACCCG GGCTGGCCTT 
CTAAGAAGAA GCTTGTTCGT AGGCTCCGAG AAAGTCTCTG ACAGGAAGTT CCTGACCCCT 
GAAGATGAAG CCAGTATTTT TACCATGGAC CACTTCCCGC TGTGGTATCG CCAGGCCTCT 
GAGCAGCCCC CCGGCAGCTT TGTCTTCAAT CTCCGCTGGG CAGAGGGACC AGATAGCCCA 
GGCAAGCCAG TGGCGGTAAG GGCCAGCACA GCAGTAACTG TGACCGTGGA TGGGAAGACG 
GCCATTGCAG CAGCTGTGGG CATCCAGATG CAAGCAGACT ACCTCCAGCG CCAGTTCTGG 
GCAGCCATGC AGCAGTGCAA CGCTGTAGAG GGGCCGTGCC TGAAGAGCTG CGAGGACACT 
GATCTCGACT GCTTCGTCAT AGACAACAAT GGCTTCGTCC TGATCTCAGA GAGACCCCAA 
GAGATGGGAA GACTTCTGGG GGAAGCGGAT GGTGCTCTCA TGAAGCAACT TCTCAGCATG 
GGGGTGTTCA GCCGTGTGAC CATGTATGAC TACCAGGCCA TGTGCAAGCC CCCAGATCAT 
CACCACAGTG CGGCCGAATC CCTGTTCAGT CCTCTCTCTG CCTTCTTGAT GGTGGCCAGG 
TGGCTACTGC ATGAATGTCT CCTATTCCTG TTAGAGTGGA GTGCCTGGGG ATCTTGGCAG 
GACAAAGGGT CAGAGGCCAA ATCTGTCTTC CATCACTCCC ACAAGCACAA GAAGCAGGAC 
CTGCTGCACC CCTGTGACAC AGAGTACCCA GTGTTCGTGC ATCAGACGGC CATCCAGGAA 
GCCAATGGCA TCATCGAGTG TGGGGGCTGC CAGAAGACAT TCGTGATGCA ACAGATTCCC 
CGAAGCAACC TGCTCCTGCT CGTGACAGAC CGCACCTGTG ACTGCAGCGC CCACTCTCCC 
ATCCTCCAGG AGGCCACAGA AGTCAAATAT AATGCCTCTG TCAAGTGTGA CAGGATGCGC 
TCCCAGAAGC CCCGGAGACG ACCAGGCTCC TGCCATGCCT TCCATCCAGA GGAGAATGCC 
CAGGACTGTG GCGGTGCTTC AGACACTCTG CCTTCATCCC CTCTGCTCCT GCTATCACTG 
GGGGCCTGGC TGCTTCCACC CCAGCTTCTG TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334356.2
CDS	183..3458
Translation	MARCPMLSSSHNQDHSGRWTAVWTSLWKTPIILWLLLSDTSLPTVRGQTTVPLETVKLWADTFGRNLYNTVTRYSGSLLLQKKYKDAEPSLKIKEVDGLELVKKFSEDMETMLRRKVEAVESLVEAAEEADLNHEFNASLVFNYYNSVLINEKDDKGNYVELGAEFLLESDAHFSNLRVNVSMSSVQLPTNVYNKDPDILNGVYMSEALNPVFVENFQRDPTLTWQYFGSSTGFFRIYPGIKWMPDENGVIAFDCRNRGWYIQAATSPKDIVILVDISGSMKGLRMAIAKHTITTILDTLGENDFVNIIAYNDYVHYIEPCFKGILVQADRDNREHFKQLVDELMVKGVGVVSQALIEAFEILKQFQESKQGSLCNQAIMLITDGAVEDYEPVFETYNWPDRKVRVFTYLIGREVTFADRMKWIACNNKGYYTQISTLADAQESVMEYLHVLSRPMVINHDHDIIWTEAYMDSRLFTSEAQSLMLLTTVAMPVFSKKNETRSHGILLGVVGSDVTLRELMKLAPRYKYREGKKLRPKPNYNSVDLSEVEWEDQAEILRTAMINGETGSHSMDVKVPLDKGKRVLFLTNDYFFTDISDTPFSLGVVLTRGHGEYILLGNTSVEEGLHDLLHPDLTLASDWIYCITDIDPDHRKLSQLEAVVRFLTGVDPDLECDEELVREVLFDAVVTAPMEAYWTALALNISEESEPGVDVAFLGTRAGLLRRSLFVGSEKVSDRKFLTPEDEASIFTMDHFPLWYRQASEQPPGSFVFNLRWAEGPDSPGKPVAVRASTAVTVTVDGKTAIAAAVGIQMQADYLQRQFWAAMQQCNAVEGPCLKSCEDTDLDCFVIDNNGFVLISERPQEMGRLLGEADGALMKQLLSMGVFSRVTMYDYQAMCKPPDHHHSAAESLFSPLSAFLMVARWLLHECLLFLLEWSAWGSWQDKGSEAKSVFHHSHKHKKQDLLHPCDTEYPVFVHQTAIQEANGIIECGGCQKTFVMQQIPRSNLLLLVTDRTCDCSAHSPILQEATEVKYNASVKCDRMRSQKPRRRPGSCHAFHPEENAQDCGGASDTLPSSPLLLLSLGAWLLPPQLLW

Target ORF information:

RefSeq Version	NM_001347427.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, alpha 2/delta subunit 4 (Cacna2d4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347427.2

ORF Insert Sequence:

ATGGCAAGAT GCCCCATGCT GTCCTCTAGC CACAACCAAG ACCACAGTGG ACGGTGGACA 
GCAGTGTGGA CCTCCCTGTG GAAGACGCCA ATCATCCTGT GGCTGCTGCT CTCGGACACC 
TCCCTGCCTA CCGTGAGGGG CCAGACCACA GTTCCTCTGG AAACAGTGAA GCTGTGGGCC 
GATACCTTCG GCAGGAATCT GTACAACACG GTGACCAGAT ATTCAGGCTC CCTCCTGCTG 
CAGAAGAAGT ACAAGGATGC AGAACCCAGT CTGAAGATCA AAGAGGTGGA TGGCTTGGAG 
CTGGTGAAGA AGTTCTCGGA GGACATGGAG ACCATGCTTC GGAGGAAAGT CGAGGCTGTT 
GAGAGCCTGG TGGAGGCCGC TGAGGAGGCT GACCTGAATC ATGAGTTCAA TGCATCTCTG 
GTGTTTAACT ACTACAACTC GGTACTAATC AATGAGAAGG ATGACAAGGG CAACTACGTG 
GAGCTGGGAG CTGAGTTTCT TCTCGAGTCC GACGCGCACT TCAGCAACCT GAGGGTGAAT 
GTCTCCATGA GCAGCGTTCA ACTGCCCACC AACGTGTACA ACAAAGACCC AGACATTTTA 
AATGGCGTGT ATATGTCCGA AGCCCTGAAC CCCGTGTTTG TGGAGAACTT TCAGAGAGAC 
CCTACATTGA CATGGCAGTA TTTCGGCAGC TCAACTGGGT TCTTCAGGAT CTATCCAGGA 
ATAAAGTGGA TGCCTGATGA AAACGGAGTC ATCGCCTTCG ACTGCAGAAA CCGTGGCTGG 
TACATACAAG CTGCCACATC TCCCAAAGAC ATCGTAATTC TGGTGGACAT TAGCGGCAGC 
ATGAAGGGGC TGAGGATGGC TATCGCCAAG CACACCATTA CCACCATATT GGACACCCTG 
GGAGAGAACG ACTTCGTGAA CATCATCGCG TACAATGACT ACGTCCACTA CATTGAGCCC 
TGCTTCAAAG GCATCCTTGT CCAAGCAGAT CGAGACAACC GAGAGCATTT CAAGCAGTTG 
GTGGATGAGC TGATGGTCAA AGGTGTGGGT GTCGTGAGTC AAGCTCTAAT TGAAGCTTTC 
GAGATCCTGA AACAGTTCCA AGAGTCCAAA CAAGGAAGTC TCTGCAACCA GGCCATCATG 
CTTATCACCG ATGGGGCTGT GGAAGACTAT GAGCCCGTGT TTGAGACCTA TAACTGGCCA 
GACCGAAAGG TCCGAGTTTT CACTTACCTT ATTGGAAGAG AAGTGACTTT CGCTGACCGC 
ATGAAGTGGA TCGCCTGCAA CAATAAAGGC TACTACACAC AGATCTCCAC ACTGGCGGAT 
GCGCAGGAGA GCGTGATGGA GTACCTGCAT GTGCTTAGCC GCCCCATGGT CATCAACCAT 
GACCACGACA TCATCTGGAC AGAGGCTTAC ATGGACAGCC GGCTCTTCAC ATCAGAGGCA 
CAGAGCCTGA TGCTCCTCAC CACAGTGGCC ATGCCTGTCT TCAGCAAAAA GAATGAAACA 
AGATCCCATG GCATTCTCCT GGGTGTAGTG GGCTCTGACG TGACCCTAAG AGAGCTCATG 
AAGCTGGCAC CCCGATATAA GTACAGAGAA GGCAAGAAGC TGAGACCCAA ACCCAACTAC 
AACAGTGTGG ACCTCTCAGA AGTGGAGTGG GAGGACCAAG CTGAAATTCT GAGGACCGCC 
ATGATCAATG GGGAAACCGG GTCTCACTCC ATGGACGTGA AGGTGCCACT GGACAAAGGG 
AAGCGAGTTC TATTCCTGAC CAATGACTAC TTCTTCACAG ACATCAGTGA CACACCTTTC 
AGCCTGGGAG TGGTGCTCAC CAGAGGTCAT GGAGAATACA TCCTCCTGGG GAACACATCT 
GTGGAAGAAG GCCTGCACGA CTTGCTTCAT CCGGATCTGA CCCTGGCCAG TGACTGGATC 
TACTGTATCA CAGATATCGA CCCGGACCAC CGGAAACTCA GCCAACTGGA AGCTGTGGTT 
CGTTTCCTGA CAGGGGTGGA TCCAGACCTG GAGTGTGACG AGGAACTGGT GCGGGAGGTG 
CTGTTTGATG CGGTGGTAAC CGCCCCCATG GAAGCCTATT GGACAGCACT GGCACTCAAC 
ATCTCTGAAG AGTCAGAGCC TGGCGTGGAT GTGGCCTTCC TGGGAACCCG GGCTGGCCTT 
CTAAGAAGAA GCTTGTTCGT AGGCTCCGAG AAAGTCTCTG ACAGGAAGTT CCTGACCCCT 
GAAGATGAAG CCAGTATTTT TACCATGGAC CACTTCCCGC TGTGGTATCG CCAGGCCTCT 
GAGCAGCCCC CCGGCAGCTT TGTCTTCAAT CTCCGCTGGG CAGAGGGACC AGATAGCCCA 
GGCAAGCCAG TGGCGGTAAG GGCCAGCACA GCAGTAACTG TGACCGTGGA TGGGAAGACG 
GCCATTGCAG CAGCTGTGGG CATCCAGATG CAAGCAGACT ACCTCCAGCG CCAGTTCTGG 
GCAGCCATGC AGCAGTGCAA CGCTGTAGAG GGGCCGTGCC TGAAGAGCTG CGAGGACACT 
GATCTCGACT GCTTCGTCAT AGACAACAAT GGCTTCGTCC TGATCTCAGA GAGACCCCAA 
GAGATGGGAA GACTTCTGGG GGAAGCGGAT GGTGCTCTCA TGAAGCAACT TCTCAGCATG 
GGGGTGTTCA GCCGTGTGAC CATGTATGAC TACCAGGCCA TGTGCAAGCC CCCAGATCAT 
CACCACAGTG CGGCCGAATC CCTGTTCAGT CCTCTCTCTG CCTTCTTGAT GGTGGCCAGG 
TGGCTACTGC ATGAATGTCT CCTATTCCTG TTAGAGTGGA GTGCCTGGGG ATCTTGGCAG 
GACAAAGGGT CAGAGGCCAA ATCTGTCTTC CATCACTCCC ACAAGCACAA GAAGCAGGAC 
CTGCTGCACC CCTGTGACAC AGAGTACCCA GTGTTCGTGC ATCAGACGGC CATCCAGGAA 
GCCAATGGCA TCATCGAGTG TGGGGGCTGC CAGAAGACAT TCGTGATGCA ACAGATTCCC 
CGAAGCAACC TGCTCCTGCT CGTGACAGAC CGCACCTGTG ACTGCAGCGC CCACTCTCCC 
ATCCTCCAGG AGGCCACAGA AGTCAAATAT AATGCCTCTG TCAAGTGTGA CAGGATGCGC 
TCCCAGAAGC CCCGGAGACG ACCAGGCTCC TGCCATGCCT TCCATCCAGA GGAGAATGCC 
CAGGACTGTG GCGGTGCTTC AGACACTCTG CCTTCATCCC CTCTGCTCCT GCTATCACTG 
GGGGCCTGGC TGCTTCCACC CCAGCTTCTG TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna2d4 ( NM_001033382.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001028554.3 Mus musculus calcium channel, voltage-dependent, alpha 2/delta subunit 4 (Cacna2d4), transcript variant 1, mRNA.

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CloneID	OMu84765
Clone ID Related Accession (Same CDS sequence)	NM_001033382.3
Accession Version	NM_001033382.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Mus musculus(house mouse)
Product	voltage-dependent calcium channel subunit alpha-2/delta-4 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC115816.9. On May 2, 2019 this sequence version replaced NM_001033382.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BK005394.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAAGAT GCCCCATGCT GTCCTCTAGC CACAACCAAG ACCACAGTGG ACGGTGGACA 
GCAGTGTGGA CCTCCCTGTG GAAGACGCCA ATCATCCTGT GGCTGCTGCT CTCGGACACC 
TCCCTGCCTA CCGTGAGGGG CCAGACCACA GTTCCTCTGG AAACAGTGAA GCTGTGGGCC 
GATACCTTCG GCAGGAATCT GTACAACACG GTGACCAGAT ATTCAGGCTC CCTCCTGCTG 
CAGAAGAAGT ACAAGGATGC AGAACCCAGT CTGAAGATCA AAGAGGTGGA TGGCTTGGAG 
CTGGTGAAGA AGTTCTCGGA GGACATGGAG ACCATGCTTC GGAGGAAAGT CGAGGCTGTT 
GAGAGCCTGG TGGAGGCCGC TGAGGAGGCT GACCTGAATC ATGAGTTCAA TGCATCTCTG 
GTGTTTAACT ACTACAACTC GGTACTAATC AATGAGAAGG ATGACAAGGG CAACTACGTG 
GAGCTGGGAG CTGAGTTTCT TCTCGAGTCC GACGCGCACT TCAGCAACCT GAGGGTGAAT 
GTCTCCATGA GCAGCGTTCA ACTGCCCACC AACGTGTACA ACAAAGACCC AGACATTTTA 
AATGGCGTGT ATATGTCCGA AGCCCTGAAC CCCGTGTTTG TGGAGAACTT TCAGAGAGAC 
CCTACATTGA CATGGCAGTA TTTCGGCAGC TCAACTGGGT TCTTCAGGAT CTATCCAGGA 
ATAAAGTGGA TGCCTGATGA AAACGGAGTC ATCGCCTTCG ACTGCAGAAA CCGTGGCTGG 
TACATACAAG CTGCCACATC TCCCAAAGAC ATCGTAATTC TGGTGGACAT TAGCGGCAGC 
ATGAAGGGGC TGAGGATGGC TATCGCCAAG CACACCATTA CCACCATATT GGACACCCTG 
GGAGAGAACG ACTTCGTGAA CATCATCGCG TACAATGACT ACGTCCACTA CATTGAGCCC 
TGCTTCAAAG GCATCCTTGT CCAAGCAGAT CGAGACAACC GAGAGCATTT CAAGCAGTTG 
GTGGATGAGC TGATGGTCAA AGGTGTGGGT GTCGTGAGTC AAGCTCTAAT TGAAGCTTTC 
GAGATCCTGA AACAGTTCCA AGAGTCCAAA CAAGGAAGTC TCTGCAACCA GGCCATCATG 
CTTATCACCG ATGGGGCTGT GGAAGACTAT GAGCCCGTGT TTGAGACCTA TAACTGGCCA 
GACCGAAAGG TCCGAGTTTT CACTTACCTT ATTGGAAGAG AAGTGACTTT CGCTGACCGC 
ATGAAGTGGA TCGCCTGCAA CAATAAAGGC TACTACACAC AGATCTCCAC ACTGGCGGAT 
GCGCAGGAGA GCGTGATGGA GTACCTGCAT GTGCTTAGCC GCCCCATGGT CATCAACCAT 
GACCACGACA TCATCTGGAC AGAGGCTTAC ATGGACAGCC GGCTCTTCAC ATCAGAGGCA 
CAGAGCCTGA TGCTCCTCAC CACAGTGGCC ATGCCTGTCT TCAGCAAAAA GAATGAAACA 
AGATCCCATG GCATTCTCCT GGGTGTAGTG GGCTCTGACG TGACCCTAAG AGAGCTCATG 
AAGCTGGCAC CCCGATATAA GCTTGGGGTG CATGGCTATG CCTTCTTGAA CACTAACAAT 
GGCTACATCC TCTCTCATCC TGACCTCCGA CCTTTGTACA GAGAAGGCAA GAAGCTGAGA 
CCCAAACCCA ACTACAACAG TGTGGACCTC TCAGAAGTGG AGTGGGAGGA CCAAGCTGAA 
ATTCTGAGGA CCGCCATGAT CAATGGGGAA ACCGGGTCTC ACTCCATGGA CGTGAAGGTG 
CCACTGGACA AAGGGAAGCG AGTTCTATTC CTGACCAATG ACTACTTCTT CACAGACATC 
AGTGACACAC CTTTCAGCCT GGGAGTGGTG CTCACCAGAG GTCATGGAGA ATACATCCTC 
CTGGGGAACA CATCTGTGGA AGAAGGCCTG CACGACTTGC TTCATCCGGA TCTGACCCTG 
GCCAGTGACT GGATCTACTG TATCACAGAT ATCGACCCGG ACCACCGGAA ACTCAGCCAA 
CTGGAAGCTG TGGTTCGTTT CCTGACAGGG GTGGATCCAG ACCTGGAGTG TGACGAGGAA 
CTGGTGCGGG AGGTGCTGTT TGATGCGGTG GTAACCGCCC CCATGGAAGC CTATTGGACA 
GCACTGGCAC TCAACATCTC TGAAGAGTCA GAGCCTGGCG TGGATGTGGC CTTCCTGGGA 
ACCCGGGCTG GCCTTCTAAG AAGAAGCTTG TTCGTAGGCT CCGAGAAAGT CTCTGACAGG 
AAGTTCCTGA CCCCTGAAGA TGAAGCCAGT ATTTTTACCA TGGACCACTT CCCGCTGTGG 
TATCGCCAGG CCTCTGAGCA GCCCCCCGGC AGCTTTGTCT TCAATCTCCG CTGGGCAGAG 
GGACCAGATA GCCCAGGCAA GCCAGTGGCG GTAAGGGCCA GCACAGCAGT AACTGTGACC 
GTGGATGGGA AGACGGCCAT TGCAGCAGCT GTGGGCATCC AGATGCAAGC AGACTACCTC 
CAGCGCCAGT TCTGGGCAGC CATGCAGCAG TGCAACGCTG TAGAGGGGCC GTGCCTGAAG 
AGCTGCGAGG ACACTGATCT CGACTGCTTC GTCATAGACA ACAATGGCTT CGTCCTGATC 
TCAGAGAGAC CCCAAGAGAT GGGAAGACTT CTGGGGGAAG CGGATGGTGC TCTCATGAAG 
CAACTTCTCA GCATGGGGGT GTTCAGCCGT GTGACCATGT ATGACTACCA GGCCATGTGC 
AAGCCCCCAG ATCATCACCA CAGTGCGGCC GAATCCCTGT TCAGTCCTCT CTCTGCCTTC 
TTGATGGTGG CCAGGTGGCT ACTGCATGAA TGTCTCCTAT TCCTGTTAGA GTGGAGTGCC 
TGGGGATCTT GGCAGGACAA AGGGTCAGAG GCCAAATCTG TCTTCCATCA CTCCCACAAG 
CACAAGAAGC AGGACCTGCT GCACCCCTGT GACACAGAGT ACCCAGTGTT CGTGCATCAG 
ACGGCCATCC AGGAAGCCAA TGGCATCATC GAGTGTGGGG GCTGCCAGAA GACATTCGTG 
ATGCAACAGA TTCCCCGAAG CAACCTGCTC CTGCTCGTGA CAGACCGCAC CTGTGACTGC 
AGCGCCCACT CTCCCATCCT CCAGGAGGCC ACAGAAGTCA AATATAATGC CTCTGTCAAG 
TGTGACAGGA TGCGCTCCCA GAAGCCCCGG AGACGACCAG GCTCCTGCCA TGCCTTCCAT 
CCAGAGGAGA ATGCCCAGGA CTGTGGCGGT GCTTCAGACA CTCTGCCTTC ATCCCCTCTG 
CTCCTGCTAT CACTGGGGGC CTGGCTGCTT CCACCCCAGC TTCTGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001028554.3
CDS	183..3533
Translation	MARCPMLSSSHNQDHSGRWTAVWTSLWKTPIILWLLLSDTSLPTVRGQTTVPLETVKLWADTFGRNLYNTVTRYSGSLLLQKKYKDAEPSLKIKEVDGLELVKKFSEDMETMLRRKVEAVESLVEAAEEADLNHEFNASLVFNYYNSVLINEKDDKGNYVELGAEFLLESDAHFSNLRVNVSMSSVQLPTNVYNKDPDILNGVYMSEALNPVFVENFQRDPTLTWQYFGSSTGFFRIYPGIKWMPDENGVIAFDCRNRGWYIQAATSPKDIVILVDISGSMKGLRMAIAKHTITTILDTLGENDFVNIIAYNDYVHYIEPCFKGILVQADRDNREHFKQLVDELMVKGVGVVSQALIEAFEILKQFQESKQGSLCNQAIMLITDGAVEDYEPVFETYNWPDRKVRVFTYLIGREVTFADRMKWIACNNKGYYTQISTLADAQESVMEYLHVLSRPMVINHDHDIIWTEAYMDSRLFTSEAQSLMLLTTVAMPVFSKKNETRSHGILLGVVGSDVTLRELMKLAPRYKLGVHGYAFLNTNNGYILSHPDLRPLYREGKKLRPKPNYNSVDLSEVEWEDQAEILRTAMINGETGSHSMDVKVPLDKGKRVLFLTNDYFFTDISDTPFSLGVVLTRGHGEYILLGNTSVEEGLHDLLHPDLTLASDWIYCITDIDPDHRKLSQLEAVVRFLTGVDPDLECDEELVREVLFDAVVTAPMEAYWTALALNISEESEPGVDVAFLGTRAGLLRRSLFVGSEKVSDRKFLTPEDEASIFTMDHFPLWYRQASEQPPGSFVFNLRWAEGPDSPGKPVAVRASTAVTVTVDGKTAIAAAVGIQMQADYLQRQFWAAMQQCNAVEGPCLKSCEDTDLDCFVIDNNGFVLISERPQEMGRLLGEADGALMKQLLSMGVFSRVTMYDYQAMCKPPDHHHSAAESLFSPLSAFLMVARWLLHECLLFLLEWSAWGSWQDKGSEAKSVFHHSHKHKKQDLLHPCDTEYPVFVHQTAIQEANGIIECGGCQKTFVMQQIPRSNLLLLVTDRTCDCSAHSPILQEATEVKYNASVKCDRMRSQKPRRRPGSCHAFHPEENAQDCGGASDTLPSSPLLLLSLGAWLLPPQLLW

Target ORF information:

RefSeq Version	NM_001033382.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, alpha 2/delta subunit 4 (Cacna2d4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033382.3

ORF Insert Sequence:

ATGGCAAGAT GCCCCATGCT GTCCTCTAGC CACAACCAAG ACCACAGTGG ACGGTGGACA 
GCAGTGTGGA CCTCCCTGTG GAAGACGCCA ATCATCCTGT GGCTGCTGCT CTCGGACACC 
TCCCTGCCTA CCGTGAGGGG CCAGACCACA GTTCCTCTGG AAACAGTGAA GCTGTGGGCC 
GATACCTTCG GCAGGAATCT GTACAACACG GTGACCAGAT ATTCAGGCTC CCTCCTGCTG 
CAGAAGAAGT ACAAGGATGC AGAACCCAGT CTGAAGATCA AAGAGGTGGA TGGCTTGGAG 
CTGGTGAAGA AGTTCTCGGA GGACATGGAG ACCATGCTTC GGAGGAAAGT CGAGGCTGTT 
GAGAGCCTGG TGGAGGCCGC TGAGGAGGCT GACCTGAATC ATGAGTTCAA TGCATCTCTG 
GTGTTTAACT ACTACAACTC GGTACTAATC AATGAGAAGG ATGACAAGGG CAACTACGTG 
GAGCTGGGAG CTGAGTTTCT TCTCGAGTCC GACGCGCACT TCAGCAACCT GAGGGTGAAT 
GTCTCCATGA GCAGCGTTCA ACTGCCCACC AACGTGTACA ACAAAGACCC AGACATTTTA 
AATGGCGTGT ATATGTCCGA AGCCCTGAAC CCCGTGTTTG TGGAGAACTT TCAGAGAGAC 
CCTACATTGA CATGGCAGTA TTTCGGCAGC TCAACTGGGT TCTTCAGGAT CTATCCAGGA 
ATAAAGTGGA TGCCTGATGA AAACGGAGTC ATCGCCTTCG ACTGCAGAAA CCGTGGCTGG 
TACATACAAG CTGCCACATC TCCCAAAGAC ATCGTAATTC TGGTGGACAT TAGCGGCAGC 
ATGAAGGGGC TGAGGATGGC TATCGCCAAG CACACCATTA CCACCATATT GGACACCCTG 
GGAGAGAACG ACTTCGTGAA CATCATCGCG TACAATGACT ACGTCCACTA CATTGAGCCC 
TGCTTCAAAG GCATCCTTGT CCAAGCAGAT CGAGACAACC GAGAGCATTT CAAGCAGTTG 
GTGGATGAGC TGATGGTCAA AGGTGTGGGT GTCGTGAGTC AAGCTCTAAT TGAAGCTTTC 
GAGATCCTGA AACAGTTCCA AGAGTCCAAA CAAGGAAGTC TCTGCAACCA GGCCATCATG 
CTTATCACCG ATGGGGCTGT GGAAGACTAT GAGCCCGTGT TTGAGACCTA TAACTGGCCA 
GACCGAAAGG TCCGAGTTTT CACTTACCTT ATTGGAAGAG AAGTGACTTT CGCTGACCGC 
ATGAAGTGGA TCGCCTGCAA CAATAAAGGC TACTACACAC AGATCTCCAC ACTGGCGGAT 
GCGCAGGAGA GCGTGATGGA GTACCTGCAT GTGCTTAGCC GCCCCATGGT CATCAACCAT 
GACCACGACA TCATCTGGAC AGAGGCTTAC ATGGACAGCC GGCTCTTCAC ATCAGAGGCA 
CAGAGCCTGA TGCTCCTCAC CACAGTGGCC ATGCCTGTCT TCAGCAAAAA GAATGAAACA 
AGATCCCATG GCATTCTCCT GGGTGTAGTG GGCTCTGACG TGACCCTAAG AGAGCTCATG 
AAGCTGGCAC CCCGATATAA GCTTGGGGTG CATGGCTATG CCTTCTTGAA CACTAACAAT 
GGCTACATCC TCTCTCATCC TGACCTCCGA CCTTTGTACA GAGAAGGCAA GAAGCTGAGA 
CCCAAACCCA ACTACAACAG TGTGGACCTC TCAGAAGTGG AGTGGGAGGA CCAAGCTGAA 
ATTCTGAGGA CCGCCATGAT CAATGGGGAA ACCGGGTCTC ACTCCATGGA CGTGAAGGTG 
CCACTGGACA AAGGGAAGCG AGTTCTATTC CTGACCAATG ACTACTTCTT CACAGACATC 
AGTGACACAC CTTTCAGCCT GGGAGTGGTG CTCACCAGAG GTCATGGAGA ATACATCCTC 
CTGGGGAACA CATCTGTGGA AGAAGGCCTG CACGACTTGC TTCATCCGGA TCTGACCCTG 
GCCAGTGACT GGATCTACTG TATCACAGAT ATCGACCCGG ACCACCGGAA ACTCAGCCAA 
CTGGAAGCTG TGGTTCGTTT CCTGACAGGG GTGGATCCAG ACCTGGAGTG TGACGAGGAA 
CTGGTGCGGG AGGTGCTGTT TGATGCGGTG GTAACCGCCC CCATGGAAGC CTATTGGACA 
GCACTGGCAC TCAACATCTC TGAAGAGTCA GAGCCTGGCG TGGATGTGGC CTTCCTGGGA 
ACCCGGGCTG GCCTTCTAAG AAGAAGCTTG TTCGTAGGCT CCGAGAAAGT CTCTGACAGG 
AAGTTCCTGA CCCCTGAAGA TGAAGCCAGT ATTTTTACCA TGGACCACTT CCCGCTGTGG 
TATCGCCAGG CCTCTGAGCA GCCCCCCGGC AGCTTTGTCT TCAATCTCCG CTGGGCAGAG 
GGACCAGATA GCCCAGGCAA GCCAGTGGCG GTAAGGGCCA GCACAGCAGT AACTGTGACC 
GTGGATGGGA AGACGGCCAT TGCAGCAGCT GTGGGCATCC AGATGCAAGC AGACTACCTC 
CAGCGCCAGT TCTGGGCAGC CATGCAGCAG TGCAACGCTG TAGAGGGGCC GTGCCTGAAG 
AGCTGCGAGG ACACTGATCT CGACTGCTTC GTCATAGACA ACAATGGCTT CGTCCTGATC 
TCAGAGAGAC CCCAAGAGAT GGGAAGACTT CTGGGGGAAG CGGATGGTGC TCTCATGAAG 
CAACTTCTCA GCATGGGGGT GTTCAGCCGT GTGACCATGT ATGACTACCA GGCCATGTGC 
AAGCCCCCAG ATCATCACCA CAGTGCGGCC GAATCCCTGT TCAGTCCTCT CTCTGCCTTC 
TTGATGGTGG CCAGGTGGCT ACTGCATGAA TGTCTCCTAT TCCTGTTAGA GTGGAGTGCC 
TGGGGATCTT GGCAGGACAA AGGGTCAGAG GCCAAATCTG TCTTCCATCA CTCCCACAAG 
CACAAGAAGC AGGACCTGCT GCACCCCTGT GACACAGAGT ACCCAGTGTT CGTGCATCAG 
ACGGCCATCC AGGAAGCCAA TGGCATCATC GAGTGTGGGG GCTGCCAGAA GACATTCGTG 
ATGCAACAGA TTCCCCGAAG CAACCTGCTC CTGCTCGTGA CAGACCGCAC CTGTGACTGC 
AGCGCCCACT CTCCCATCCT CCAGGAGGCC ACAGAAGTCA AATATAATGC CTCTGTCAAG 
TGTGACAGGA TGCGCTCCCA GAAGCCCCGG AGACGACCAG GCTCCTGCCA TGCCTTCCAT 
CCAGAGGAGA ATGCCCAGGA CTGTGGCGGT GCTTCAGACA CTCTGCCTTC ATCCCCTCTG 
CTCCTGCTAT CACTGGGGGC CTGGCTGCTT CCACCCCAGC TTCTGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna2d4 ( NM_001033382.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001028554.2 Mus musculus calcium channel, voltage-dependent, alpha 2/delta subunit 4 (Cacna2d4), transcript variant 1, mRNA.

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CloneID	OMu12003
Clone ID Related Accession (Same CDS sequence)	NM_001033382.2
Accession Version	NM_001033382.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-19
Organism	Mus musculus(house mouse)
Product	voltage-dependent calcium channel subunit alpha-2/delta-4 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BK005394.1 and AC115816.9. On Feb 7, 2007 this sequence version replaced NM_001033382.1. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BK005394.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCAGGA GTTCCTGTGG CAGCAAGTTA TCTCCCAGCA GGGGCTCTGT CCTCCTCCCC 
TGCCCCTCCC CCAGTGGGAA CACCATGGCA AGATGCCCCA TGCTGTCCTC TAGCCACAAC 
CAAGACCACA GTGGACGGTG GACAGCAGTG TGGACCTCCC TGTGGAAGAC GCCAATCATC 
CTGTGGCTGC TGCTCTCGGA CACCTCCCTG CCTACCGTGA GGGGCCAGAC CACAGTTCCT 
CTGGAAACAG TGAAGCTGTG GGCCGATACC TTCGGCAGGA ATCTGTACAA CACGGTGACC 
AGATATTCAG GCTCCCTCCT GCTGCAGAAG AAGTACAAGG ATGCAGAACC CAGTCTGAAG 
ATCAAAGAGG TGGATGGCTT GGAGCTGGTG AAGAAGTTCT CGGAGGACAT GGAGACCATG 
CTTCGGAGGA AAGTCGAGGC TGTTGAGAGC CTGGTGGAGG CCGCTGAGGA GGCTGACCTG 
AATCATGAGT TCAATGCATC TCTGGTGTTT AACTACTACA ACTCGGTACT AATCAATGAG 
AAGGATGACA AGGGCAACTA CGTGGAGCTG GGAGCTGAGT TTCTTCTCGA GTCCGACGCG 
CACTTCAGCA ACCTGAGGGT GAATGTCTCC ATGAGCAGCG TTCAACTGCC CACCAACGTG 
TACAACAAAG ACCCAGACAT TTTAAATGGC GTGTATATGT CCGAAGCCCT GAACCCCGTG 
TTTGTGGAGA ACTTTCAGAG AGACCCTACA TTGACATGGC AGTATTTCGG CAGCTCAACT 
GGGTTCTTCA GGATCTATCC AGGAATAAAG TGGATGCCTG ATGAAAACGG AGTCATCGCC 
TTCGACTGCA GAAACCGTGG CTGGTACATA CAAGCTGCCA CATCTCCCAA AGACATCGTA 
ATTCTGGTGG ACATTAGCGG CAGCATGAAG GGGCTGAGGA TGGCTATCGC CAAGCACACC 
ATTACCACCA TATTGGACAC CCTGGGAGAG AACGACTTCG TGAACATCAT CGCGTACAAT 
GACTACGTCC ACTACATTGA GCCCTGCTTC AAAGGCATCC TTGTCCAAGC AGATCGAGAC 
AACCGAGAGC ATTTCAAGCA GTTGGTGGAT GAGCTGATGG TCAAAGGTGT GGGTGTCGTG 
AGTCAAGCTC TAATTGAAGC TTTCGAGATC CTGAAACAGT TCCAAGAGTC CAAACAAGGA 
AGTCTCTGCA ACCAGGCCAT CATGCTTATC ACCGATGGGG CTGTGGAAGA CTATGAGCCC 
GTGTTTGAGA CCTATAACTG GCCAGACCGA AAGGTCCGAG TTTTCACTTA CCTTATTGGA 
AGAGAAGTGA CTTTCGCTGA CCGCATGAAG TGGATCGCCT GCAACAATAA AGGCTACTAC 
ACACAGATCT CCACACTGGC GGATGCGCAG GAGAGCGTGA TGGAGTACCT GCATGTGCTT 
AGCCGCCCCA TGGTCATCAA CCATGACCAC GACATCATCT GGACAGAGGC TTACATGGAC 
AGCCGGCTCT TCACATCAGA GGCACAGAGC CTGATGCTCC TCACCACAGT GGCCATGCCT 
GTCTTCAGCA AAAAGAATGA AACAAGATCC CATGGCATTC TCCTGGGTGT AGTGGGCTCT 
GACGTGACCC TAAGAGAGCT CATGAAGCTG GCACCCCGAT ATAAGCTTGG GGTGCATGGC 
TATGCCTTCT TGAACACTAA CAATGGCTAC ATCCTCTCTC ATCCTGACCT CCGACCTTTG 
TACAGAGAAG GCAAGAAGCT GAGACCCAAA CCCAACTACA ACAGTGTGGA CCTCTCAGAA 
GTGGAGTGGG AGGACCAAGC TGAAATTCTG AGGACCGCCA TGATCAATGG GGAAACCGGG 
TCTCACTCCA TGGACGTGAA GGTGCCACTG GACAAAGGGA AGCGAGTTCT ATTCCTGACC 
AATGACTACT TCTTCACAGA CATCAGTGAC ACACCTTTCA GCCTGGGAGT GGTGCTCACC 
AGAGGTCATG GAGAATACAT CCTCCTGGGG AACACATCTG TGGAAGAAGG CCTGCACGAC 
TTGCTTCATC CGGATCTGAC CCTGGCCAGT GACTGGATCT ACTGTATCAC AGATATCGAC 
CCGGACCACC GGAAACTCAG CCAACTGGAA GCTGTGGTTC GTTTCCTGAC AGGGGTGGAT 
CCAGACCTGG AGTGTGACGA GGAACTGGTG CGGGAGGTGC TGTTTGATGC GGTGGTAACC 
GCCCCCATGG AAGCCTATTG GACAGCACTG GCACTCAACA TCTCTGAAGA GTCAGAGCCT 
GGCGTGGATG TGGCCTTCCT GGGAACCCGG GCTGGCCTTC TAAGAAGAAG CTTGTTCGTA 
GGCTCCGAGA AAGTCTCTGA CAGGAAGTTC CTGACCCCTG AAGATGAAGC CAGTATTTTT 
ACCATGGACC ACTTCCCGCT GTGGTATCGC CAGGCCTCTG AGCAGCCCCC CGGCAGCTTT 
GTCTTCAATC TCCGCTGGGC AGAGGGACCA GATAGCCCAG GCAAGCCAGT GGCGGTAAGG 
GCCAGCACAG CAGTAACTGT GACCGTGGAT GGGAAGACGG CCATTGCAGC AGCTGTGGGC 
ATCCAGATGC AAGCAGACTA CCTCCAGCGC CAGTTCTGGG CAGCCATGCA GCAGTGCAAC 
GCTGTAGAGG GGCCGTGCCT GAAGAGCTGC GAGGACACTG ATCTCGACTG CTTCGTCATA 
GACAACAATG GCTTCGTCCT GATCTCAGAG AGACCCCAAG AGATGGGAAG ACTTCTGGGG 
GAAGCGGATG GTGCTCTCAT GAAGCAACTT CTCAGCATGG GGGTGTTCAG CCGTGTGACC 
ATGTATGACT ACCAGGCCAT GTGCAAGCCC CCAGATCATC ACCACAGTGC GGCCGAATCC 
CTGTTCAGTC CTCTCTCTGC CTTCTTGATG GTGGCCAGGT GGCTACTGCA TGAATGTCTC 
CTATTCCTGT TAGAGTGGAG TGCCTGGGGA TCTTGGCAGG ACAAAGGGTC AGAGGCCAAA 
TCTGTCTTCC ATCACTCCCA CAAGCACAAG AAGCAGGACC TGCTGCACCC CTGTGACACA 
GAGTACCCAG TGTTCGTGCA TCAGACGGCC ATCCAGGAAG CCAATGGCAT CATCGAGTGT 
GGGGGCTGCC AGAAGACATT CGTGATGCAA CAGATTCCCC GAAGCAACCT GCTCCTGCTC 
GTGACAGACC GCACCTGTGA CTGCAGCGCC CACTCTCCCA TCCTCCAGGA GGCCACAGAA 
GTCAAATATA ATGCCTCTGT CAAGTGTGAC AGGATGCGCT CCCAGAAGCC CCGGAGACGA 
CCAGGCTCCT GCCATGCCTT CCATCCAGAG GAGAATGCCC AGGACTGTGG CGGTGCTTCA 
GACACTCTGC CTTCATCCCC TCTGCTCCTG CTATCACTGG GGGCCTGGCT GCTTCCACCC 
CAGCTTCTGT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001028554.2
CDS	97..3531
Translation	MPRSSCGSKLSPSRGSVLLPCPSPSGNTMARCPMLSSSHNQDHSGRWTAVWTSLWKTPIILWLLLSDTSLPTVRGQTTVPLETVKLWADTFGRNLYNTVTRYSGSLLLQKKYKDAEPSLKIKEVDGLELVKKFSEDMETMLRRKVEAVESLVEAAEEADLNHEFNASLVFNYYNSVLINEKDDKGNYVELGAEFLLESDAHFSNLRVNVSMSSVQLPTNVYNKDPDILNGVYMSEALNPVFVENFQRDPTLTWQYFGSSTGFFRIYPGIKWMPDENGVIAFDCRNRGWYIQAATSPKDIVILVDISGSMKGLRMAIAKHTITTILDTLGENDFVNIIAYNDYVHYIEPCFKGILVQADRDNREHFKQLVDELMVKGVGVVSQALIEAFEILKQFQESKQGSLCNQAIMLITDGAVEDYEPVFETYNWPDRKVRVFTYLIGREVTFADRMKWIACNNKGYYTQISTLADAQESVMEYLHVLSRPMVINHDHDIIWTEAYMDSRLFTSEAQSLMLLTTVAMPVFSKKNETRSHGILLGVVGSDVTLRELMKLAPRYKLGVHGYAFLNTNNGYILSHPDLRPLYREGKKLRPKPNYNSVDLSEVEWEDQAEILRTAMINGETGSHSMDVKVPLDKGKRVLFLTNDYFFTDISDTPFSLGVVLTRGHGEYILLGNTSVEEGLHDLLHPDLTLASDWIYCITDIDPDHRKLSQLEAVVRFLTGVDPDLECDEELVREVLFDAVVTAPMEAYWTALALNISEESEPGVDVAFLGTRAGLLRRSLFVGSEKVSDRKFLTPEDEASIFTMDHFPLWYRQASEQPPGSFVFNLRWAEGPDSPGKPVAVRASTAVTVTVDGKTAIAAAVGIQMQADYLQRQFWAAMQQCNAVEGPCLKSCEDTDLDCFVIDNNGFVLISERPQEMGRLLGEADGALMKQLLSMGVFSRVTMYDYQAMCKPPDHHHSAAESLFSPLSAFLMVARWLLHECLLFLLEWSAWGSWQDKGSEAKSVFHHSHKHKKQDLLHPCDTEYPVFVHQTAIQEANGIIECGGCQKTFVMQQIPRSNLLLLVTDRTCDCSAHSPILQEATEVKYNASVKCDRMRSQKPRRRPGSCHAFHPEENAQDCGGASDTLPSSPLLLLSLGAWLLPPQLLW

Target ORF information:

RefSeq Version	NM_001033382.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, alpha 2/delta subunit 4 (Cacna2d4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033382.2

ORF Insert Sequence:

ATGCCCAGGA GTTCCTGTGG CAGCAAGTTA TCTCCCAGCA GGGGCTCTGT CCTCCTCCCC 
TGCCCCTCCC CCAGTGGGAA CACCATGGCA AGATGCCCCA TGCTGTCCTC TAGCCACAAC 
CAAGACCACA GTGGACGGTG GACAGCAGTG TGGACCTCCC TGTGGAAGAC GCCAATCATC 
CTGTGGCTGC TGCTCTCGGA CACCTCCCTG CCTACCGTGA GGGGCCAGAC CACAGTTCCT 
CTGGAAACAG TGAAGCTGTG GGCCGATACC TTCGGCAGGA ATCTGTACAA CACGGTGACC 
AGATATTCAG GCTCCCTCCT GCTGCAGAAG AAGTACAAGG ATGCAGAACC CAGTCTGAAG 
ATCAAAGAGG TGGATGGCTT GGAGCTGGTG AAGAAGTTCT CGGAGGACAT GGAGACCATG 
CTTCGGAGGA AAGTCGAGGC TGTTGAGAGC CTGGTGGAGG CCGCTGAGGA GGCTGACCTG 
AATCATGAGT TCAATGCATC TCTGGTGTTT AACTACTACA ACTCGGTACT AATCAATGAG 
AAGGATGACA AGGGCAACTA CGTGGAGCTG GGAGCTGAGT TTCTTCTCGA GTCCGACGCG 
CACTTCAGCA ACCTGAGGGT GAATGTCTCC ATGAGCAGCG TTCAACTGCC CACCAACGTG 
TACAACAAAG ACCCAGACAT TTTAAATGGC GTGTATATGT CCGAAGCCCT GAACCCCGTG 
TTTGTGGAGA ACTTTCAGAG AGACCCTACA TTGACATGGC AGTATTTCGG CAGCTCAACT 
GGGTTCTTCA GGATCTATCC AGGAATAAAG TGGATGCCTG ATGAAAACGG AGTCATCGCC 
TTCGACTGCA GAAACCGTGG CTGGTACATA CAAGCTGCCA CATCTCCCAA AGACATCGTA 
ATTCTGGTGG ACATTAGCGG CAGCATGAAG GGGCTGAGGA TGGCTATCGC CAAGCACACC 
ATTACCACCA TATTGGACAC CCTGGGAGAG AACGACTTCG TGAACATCAT CGCGTACAAT 
GACTACGTCC ACTACATTGA GCCCTGCTTC AAAGGCATCC TTGTCCAAGC AGATCGAGAC 
AACCGAGAGC ATTTCAAGCA GTTGGTGGAT GAGCTGATGG TCAAAGGTGT GGGTGTCGTG 
AGTCAAGCTC TAATTGAAGC TTTCGAGATC CTGAAACAGT TCCAAGAGTC CAAACAAGGA 
AGTCTCTGCA ACCAGGCCAT CATGCTTATC ACCGATGGGG CTGTGGAAGA CTATGAGCCC 
GTGTTTGAGA CCTATAACTG GCCAGACCGA AAGGTCCGAG TTTTCACTTA CCTTATTGGA 
AGAGAAGTGA CTTTCGCTGA CCGCATGAAG TGGATCGCCT GCAACAATAA AGGCTACTAC 
ACACAGATCT CCACACTGGC GGATGCGCAG GAGAGCGTGA TGGAGTACCT GCATGTGCTT 
AGCCGCCCCA TGGTCATCAA CCATGACCAC GACATCATCT GGACAGAGGC TTACATGGAC 
AGCCGGCTCT TCACATCAGA GGCACAGAGC CTGATGCTCC TCACCACAGT GGCCATGCCT 
GTCTTCAGCA AAAAGAATGA AACAAGATCC CATGGCATTC TCCTGGGTGT AGTGGGCTCT 
GACGTGACCC TAAGAGAGCT CATGAAGCTG GCACCCCGAT ATAAGCTTGG GGTGCATGGC 
TATGCCTTCT TGAACACTAA CAATGGCTAC ATCCTCTCTC ATCCTGACCT CCGACCTTTG 
TACAGAGAAG GCAAGAAGCT GAGACCCAAA CCCAACTACA ACAGTGTGGA CCTCTCAGAA 
GTGGAGTGGG AGGACCAAGC TGAAATTCTG AGGACCGCCA TGATCAATGG GGAAACCGGG 
TCTCACTCCA TGGACGTGAA GGTGCCACTG GACAAAGGGA AGCGAGTTCT ATTCCTGACC 
AATGACTACT TCTTCACAGA CATCAGTGAC ACACCTTTCA GCCTGGGAGT GGTGCTCACC 
AGAGGTCATG GAGAATACAT CCTCCTGGGG AACACATCTG TGGAAGAAGG CCTGCACGAC 
TTGCTTCATC CGGATCTGAC CCTGGCCAGT GACTGGATCT ACTGTATCAC AGATATCGAC 
CCGGACCACC GGAAACTCAG CCAACTGGAA GCTGTGGTTC GTTTCCTGAC AGGGGTGGAT 
CCAGACCTGG AGTGTGACGA GGAACTGGTG CGGGAGGTGC TGTTTGATGC GGTGGTAACC 
GCCCCCATGG AAGCCTATTG GACAGCACTG GCACTCAACA TCTCTGAAGA GTCAGAGCCT 
GGCGTGGATG TGGCCTTCCT GGGAACCCGG GCTGGCCTTC TAAGAAGAAG CTTGTTCGTA 
GGCTCCGAGA AAGTCTCTGA CAGGAAGTTC CTGACCCCTG AAGATGAAGC CAGTATTTTT 
ACCATGGACC ACTTCCCGCT GTGGTATCGC CAGGCCTCTG AGCAGCCCCC CGGCAGCTTT 
GTCTTCAATC TCCGCTGGGC AGAGGGACCA GATAGCCCAG GCAAGCCAGT GGCGGTAAGG 
GCCAGCACAG CAGTAACTGT GACCGTGGAT GGGAAGACGG CCATTGCAGC AGCTGTGGGC 
ATCCAGATGC AAGCAGACTA CCTCCAGCGC CAGTTCTGGG CAGCCATGCA GCAGTGCAAC 
GCTGTAGAGG GGCCGTGCCT GAAGAGCTGC GAGGACACTG ATCTCGACTG CTTCGTCATA 
GACAACAATG GCTTCGTCCT GATCTCAGAG AGACCCCAAG AGATGGGAAG ACTTCTGGGG 
GAAGCGGATG GTGCTCTCAT GAAGCAACTT CTCAGCATGG GGGTGTTCAG CCGTGTGACC 
ATGTATGACT ACCAGGCCAT GTGCAAGCCC CCAGATCATC ACCACAGTGC GGCCGAATCC 
CTGTTCAGTC CTCTCTCTGC CTTCTTGATG GTGGCCAGGT GGCTACTGCA TGAATGTCTC 
CTATTCCTGT TAGAGTGGAG TGCCTGGGGA TCTTGGCAGG ACAAAGGGTC AGAGGCCAAA 
TCTGTCTTCC ATCACTCCCA CAAGCACAAG AAGCAGGACC TGCTGCACCC CTGTGACACA 
GAGTACCCAG TGTTCGTGCA TCAGACGGCC ATCCAGGAAG CCAATGGCAT CATCGAGTGT 
GGGGGCTGCC AGAAGACATT CGTGATGCAA CAGATTCCCC GAAGCAACCT GCTCCTGCTC 
GTGACAGACC GCACCTGTGA CTGCAGCGCC CACTCTCCCA TCCTCCAGGA GGCCACAGAA 
GTCAAATATA ATGCCTCTGT CAAGTGTGAC AGGATGCGCT CCCAGAAGCC CCGGAGACGA 
CCAGGCTCCT GCCATGCCTT CCATCCAGAG GAGAATGCCC AGGACTGTGG CGGTGCTTCA 
GACACTCTGC CTTCATCCCC TCTGCTCCTG CTATCACTGG GGGCCTGGCT GCTTCCACCC 
CAGCTTCTGT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna2d4 ( NM_001347427.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001334356.1 Mus musculus calcium channel, voltage-dependent, alpha 2/delta subunit 4 (Cacna2d4), transcript variant 2, mRNA.

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CloneID	OMu37354
Clone ID Related Accession (Same CDS sequence)	NM_001347427.1
Accession Version	NM_001347427.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Mus musculus(house mouse)
Product	voltage-dependent calcium channel subunit alpha-2/delta-4 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC115816.9. On Nov 26, 2016 this sequence version replaced XM_006506251.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes isoform 2 which is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC141091.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164136 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCAGGA GTTCCTGTGG CAGCAAGTTA TCTCCCAGCA GGGGCTCTGT CCTCCTCCCC 
TGCCCCTCCC CCAGTGGGAA CACCATGGCA AGATGCCCCA TGCTGTCCTC TAGCCACAAC 
CAAGACCACA GTGGACGGTG GACAGCAGTG TGGACCTCCC TGTGGAAGAC GCCAATCATC 
CTGTGGCTGC TGCTCTCGGA CACCTCCCTG CCTACCGTGA GGGGCCAGAC CACAGTTCCT 
CTGGAAACAG TGAAGCTGTG GGCCGATACC TTCGGCAGGA ATCTGTACAA CACGGTGACC 
AGATATTCAG GCTCCCTCCT GCTGCAGAAG AAGTACAAGG ATGCAGAACC CAGTCTGAAG 
ATCAAAGAGG TGGATGGCTT GGAGCTGGTG AAGAAGTTCT CGGAGGACAT GGAGACCATG 
CTTCGGAGGA AAGTCGAGGC TGTTGAGAGC CTGGTGGAGG CCGCTGAGGA GGCTGACCTG 
AATCATGAGT TCAATGCATC TCTGGTGTTT AACTACTACA ACTCGGTACT AATCAATGAG 
AAGGATGACA AGGGCAACTA CGTGGAGCTG GGAGCTGAGT TTCTTCTCGA GTCCGACGCG 
CACTTCAGCA ACCTGAGGGT GAATGTCTCC ATGAGCAGCG TTCAACTGCC CACCAACGTG 
TACAACAAAG ACCCAGACAT TTTAAATGGC GTGTATATGT CCGAAGCCCT GAACCCCGTG 
TTTGTGGAGA ACTTTCAGAG AGACCCTACA TTGACATGGC AGTATTTCGG CAGCTCAACT 
GGGTTCTTCA GGATCTATCC AGGAATAAAG TGGATGCCTG ATGAAAACGG AGTCATCGCC 
TTCGACTGCA GAAACCGTGG CTGGTACATA CAAGCTGCCA CATCTCCCAA AGACATCGTA 
ATTCTGGTGG ACATTAGCGG CAGCATGAAG GGGCTGAGGA TGGCTATCGC CAAGCACACC 
ATTACCACCA TATTGGACAC CCTGGGAGAG AACGACTTCG TGAACATCAT CGCGTACAAT 
GACTACGTCC ACTACATTGA GCCCTGCTTC AAAGGCATCC TTGTCCAAGC AGATCGAGAC 
AACCGAGAGC ATTTCAAGCA GTTGGTGGAT GAGCTGATGG TCAAAGGTGT GGGTGTCGTG 
AGTCAAGCTC TAATTGAAGC TTTCGAGATC CTGAAACAGT TCCAAGAGTC CAAACAAGGA 
AGTCTCTGCA ACCAGGCCAT CATGCTTATC ACCGATGGGG CTGTGGAAGA CTATGAGCCC 
GTGTTTGAGA CCTATAACTG GCCAGACCGA AAGGTCCGAG TTTTCACTTA CCTTATTGGA 
AGAGAAGTGA CTTTCGCTGA CCGCATGAAG TGGATCGCCT GCAACAATAA AGGCTACTAC 
ACACAGATCT CCACACTGGC GGATGCGCAG GAGAGCGTGA TGGAGTACCT GCATGTGCTT 
AGCCGCCCCA TGGTCATCAA CCATGACCAC GACATCATCT GGACAGAGGC TTACATGGAC 
AGCCGGCTCT TCACATCAGA GGCACAGAGC CTGATGCTCC TCACCACAGT GGCCATGCCT 
GTCTTCAGCA AAAAGAATGA AACAAGATCC CATGGCATTC TCCTGGGTGT AGTGGGCTCT 
GACGTGACCC TAAGAGAGCT CATGAAGCTG GCACCCCGAT ATAAGTACAG AGAAGGCAAG 
AAGCTGAGAC CCAAACCCAA CTACAACAGT GTGGACCTCT CAGAAGTGGA GTGGGAGGAC 
CAAGCTGAAA TTCTGAGGAC CGCCATGATC AATGGGGAAA CCGGGTCTCA CTCCATGGAC 
GTGAAGGTGC CACTGGACAA AGGGAAGCGA GTTCTATTCC TGACCAATGA CTACTTCTTC 
ACAGACATCA GTGACACACC TTTCAGCCTG GGAGTGGTGC TCACCAGAGG TCATGGAGAA 
TACATCCTCC TGGGGAACAC ATCTGTGGAA GAAGGCCTGC ACGACTTGCT TCATCCGGAT 
CTGACCCTGG CCAGTGACTG GATCTACTGT ATCACAGATA TCGACCCGGA CCACCGGAAA 
CTCAGCCAAC TGGAAGCTGT GGTTCGTTTC CTGACAGGGG TGGATCCAGA CCTGGAGTGT 
GACGAGGAAC TGGTGCGGGA GGTGCTGTTT GATGCGGTGG TAACCGCCCC CATGGAAGCC 
TATTGGACAG CACTGGCACT CAACATCTCT GAAGAGTCAG AGCCTGGCGT GGATGTGGCC 
TTCCTGGGAA CCCGGGCTGG CCTTCTAAGA AGAAGCTTGT TCGTAGGCTC CGAGAAAGTC 
TCTGACAGGA AGTTCCTGAC CCCTGAAGAT GAAGCCAGTA TTTTTACCAT GGACCACTTC 
CCGCTGTGGT ATCGCCAGGC CTCTGAGCAG CCCCCCGGCA GCTTTGTCTT CAATCTCCGC 
TGGGCAGAGG GACCAGATAG CCCAGGCAAG CCAGTGGCGG TAAGGGCCAG CACAGCAGTA 
ACTGTGACCG TGGATGGGAA GACGGCCATT GCAGCAGCTG TGGGCATCCA GATGCAAGCA 
GACTACCTCC AGCGCCAGTT CTGGGCAGCC ATGCAGCAGT GCAACGCTGT AGAGGGGCCG 
TGCCTGAAGA GCTGCGAGGA CACTGATCTC GACTGCTTCG TCATAGACAA CAATGGCTTC 
GTCCTGATCT CAGAGAGACC CCAAGAGATG GGAAGACTTC TGGGGGAAGC GGATGGTGCT 
CTCATGAAGC AACTTCTCAG CATGGGGGTG TTCAGCCGTG TGACCATGTA TGACTACCAG 
GCCATGTGCA AGCCCCCAGA TCATCACCAC AGTGCGGCCG AATCCCTGTT CAGTCCTCTC 
TCTGCCTTCT TGATGGTGGC CAGGTGGCTA CTGCATGAAT GTCTCCTATT CCTGTTAGAG 
TGGAGTGCCT GGGGATCTTG GCAGGACAAA GGGTCAGAGG CCAAATCTGT CTTCCATCAC 
TCCCACAAGC ACAAGAAGCA GGACCTGCTG CACCCCTGTG ACACAGAGTA CCCAGTGTTC 
GTGCATCAGA CGGCCATCCA GGAAGCCAAT GGCATCATCG AGTGTGGGGG CTGCCAGAAG 
ACATTCGTGA TGCAACAGAT TCCCCGAAGC AACCTGCTCC TGCTCGTGAC AGACCGCACC 
TGTGACTGCA GCGCCCACTC TCCCATCCTC CAGGAGGCCA CAGAAGTCAA ATATAATGCC 
TCTGTCAAGT GTGACAGGAT GCGCTCCCAG AAGCCCCGGA GACGACCAGG CTCCTGCCAT 
GCCTTCCATC CAGAGGAGAA TGCCCAGGAC TGTGGCGGTG CTTCAGACAC TCTGCCTTCA 
TCCCCTCTGC TCCTGCTATC ACTGGGGGCC TGGCTGCTTC CACCCCAGCT TCTGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334356.1
CDS	97..3456
Translation	MPRSSCGSKLSPSRGSVLLPCPSPSGNTMARCPMLSSSHNQDHSGRWTAVWTSLWKTPIILWLLLSDTSLPTVRGQTTVPLETVKLWADTFGRNLYNTVTRYSGSLLLQKKYKDAEPSLKIKEVDGLELVKKFSEDMETMLRRKVEAVESLVEAAEEADLNHEFNASLVFNYYNSVLINEKDDKGNYVELGAEFLLESDAHFSNLRVNVSMSSVQLPTNVYNKDPDILNGVYMSEALNPVFVENFQRDPTLTWQYFGSSTGFFRIYPGIKWMPDENGVIAFDCRNRGWYIQAATSPKDIVILVDISGSMKGLRMAIAKHTITTILDTLGENDFVNIIAYNDYVHYIEPCFKGILVQADRDNREHFKQLVDELMVKGVGVVSQALIEAFEILKQFQESKQGSLCNQAIMLITDGAVEDYEPVFETYNWPDRKVRVFTYLIGREVTFADRMKWIACNNKGYYTQISTLADAQESVMEYLHVLSRPMVINHDHDIIWTEAYMDSRLFTSEAQSLMLLTTVAMPVFSKKNETRSHGILLGVVGSDVTLRELMKLAPRYKYREGKKLRPKPNYNSVDLSEVEWEDQAEILRTAMINGETGSHSMDVKVPLDKGKRVLFLTNDYFFTDISDTPFSLGVVLTRGHGEYILLGNTSVEEGLHDLLHPDLTLASDWIYCITDIDPDHRKLSQLEAVVRFLTGVDPDLECDEELVREVLFDAVVTAPMEAYWTALALNISEESEPGVDVAFLGTRAGLLRRSLFVGSEKVSDRKFLTPEDEASIFTMDHFPLWYRQASEQPPGSFVFNLRWAEGPDSPGKPVAVRASTAVTVTVDGKTAIAAAVGIQMQADYLQRQFWAAMQQCNAVEGPCLKSCEDTDLDCFVIDNNGFVLISERPQEMGRLLGEADGALMKQLLSMGVFSRVTMYDYQAMCKPPDHHHSAAESLFSPLSAFLMVARWLLHECLLFLLEWSAWGSWQDKGSEAKSVFHHSHKHKKQDLLHPCDTEYPVFVHQTAIQEANGIIECGGCQKTFVMQQIPRSNLLLLVTDRTCDCSAHSPILQEATEVKYNASVKCDRMRSQKPRRRPGSCHAFHPEENAQDCGGASDTLPSSPLLLLSLGAWLLPPQLLW

Target ORF information:

RefSeq Version	NM_001347427.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, alpha 2/delta subunit 4 (Cacna2d4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347427.1

ORF Insert Sequence:

ATGCCCAGGA GTTCCTGTGG CAGCAAGTTA TCTCCCAGCA GGGGCTCTGT CCTCCTCCCC 
TGCCCCTCCC CCAGTGGGAA CACCATGGCA AGATGCCCCA TGCTGTCCTC TAGCCACAAC 
CAAGACCACA GTGGACGGTG GACAGCAGTG TGGACCTCCC TGTGGAAGAC GCCAATCATC 
CTGTGGCTGC TGCTCTCGGA CACCTCCCTG CCTACCGTGA GGGGCCAGAC CACAGTTCCT 
CTGGAAACAG TGAAGCTGTG GGCCGATACC TTCGGCAGGA ATCTGTACAA CACGGTGACC 
AGATATTCAG GCTCCCTCCT GCTGCAGAAG AAGTACAAGG ATGCAGAACC CAGTCTGAAG 
ATCAAAGAGG TGGATGGCTT GGAGCTGGTG AAGAAGTTCT CGGAGGACAT GGAGACCATG 
CTTCGGAGGA AAGTCGAGGC TGTTGAGAGC CTGGTGGAGG CCGCTGAGGA GGCTGACCTG 
AATCATGAGT TCAATGCATC TCTGGTGTTT AACTACTACA ACTCGGTACT AATCAATGAG 
AAGGATGACA AGGGCAACTA CGTGGAGCTG GGAGCTGAGT TTCTTCTCGA GTCCGACGCG 
CACTTCAGCA ACCTGAGGGT GAATGTCTCC ATGAGCAGCG TTCAACTGCC CACCAACGTG 
TACAACAAAG ACCCAGACAT TTTAAATGGC GTGTATATGT CCGAAGCCCT GAACCCCGTG 
TTTGTGGAGA ACTTTCAGAG AGACCCTACA TTGACATGGC AGTATTTCGG CAGCTCAACT 
GGGTTCTTCA GGATCTATCC AGGAATAAAG TGGATGCCTG ATGAAAACGG AGTCATCGCC 
TTCGACTGCA GAAACCGTGG CTGGTACATA CAAGCTGCCA CATCTCCCAA AGACATCGTA 
ATTCTGGTGG ACATTAGCGG CAGCATGAAG GGGCTGAGGA TGGCTATCGC CAAGCACACC 
ATTACCACCA TATTGGACAC CCTGGGAGAG AACGACTTCG TGAACATCAT CGCGTACAAT 
GACTACGTCC ACTACATTGA GCCCTGCTTC AAAGGCATCC TTGTCCAAGC AGATCGAGAC 
AACCGAGAGC ATTTCAAGCA GTTGGTGGAT GAGCTGATGG TCAAAGGTGT GGGTGTCGTG 
AGTCAAGCTC TAATTGAAGC TTTCGAGATC CTGAAACAGT TCCAAGAGTC CAAACAAGGA 
AGTCTCTGCA ACCAGGCCAT CATGCTTATC ACCGATGGGG CTGTGGAAGA CTATGAGCCC 
GTGTTTGAGA CCTATAACTG GCCAGACCGA AAGGTCCGAG TTTTCACTTA CCTTATTGGA 
AGAGAAGTGA CTTTCGCTGA CCGCATGAAG TGGATCGCCT GCAACAATAA AGGCTACTAC 
ACACAGATCT CCACACTGGC GGATGCGCAG GAGAGCGTGA TGGAGTACCT GCATGTGCTT 
AGCCGCCCCA TGGTCATCAA CCATGACCAC GACATCATCT GGACAGAGGC TTACATGGAC 
AGCCGGCTCT TCACATCAGA GGCACAGAGC CTGATGCTCC TCACCACAGT GGCCATGCCT 
GTCTTCAGCA AAAAGAATGA AACAAGATCC CATGGCATTC TCCTGGGTGT AGTGGGCTCT 
GACGTGACCC TAAGAGAGCT CATGAAGCTG GCACCCCGAT ATAAGTACAG AGAAGGCAAG 
AAGCTGAGAC CCAAACCCAA CTACAACAGT GTGGACCTCT CAGAAGTGGA GTGGGAGGAC 
CAAGCTGAAA TTCTGAGGAC CGCCATGATC AATGGGGAAA CCGGGTCTCA CTCCATGGAC 
GTGAAGGTGC CACTGGACAA AGGGAAGCGA GTTCTATTCC TGACCAATGA CTACTTCTTC 
ACAGACATCA GTGACACACC TTTCAGCCTG GGAGTGGTGC TCACCAGAGG TCATGGAGAA 
TACATCCTCC TGGGGAACAC ATCTGTGGAA GAAGGCCTGC ACGACTTGCT TCATCCGGAT 
CTGACCCTGG CCAGTGACTG GATCTACTGT ATCACAGATA TCGACCCGGA CCACCGGAAA 
CTCAGCCAAC TGGAAGCTGT GGTTCGTTTC CTGACAGGGG TGGATCCAGA CCTGGAGTGT 
GACGAGGAAC TGGTGCGGGA GGTGCTGTTT GATGCGGTGG TAACCGCCCC CATGGAAGCC 
TATTGGACAG CACTGGCACT CAACATCTCT GAAGAGTCAG AGCCTGGCGT GGATGTGGCC 
TTCCTGGGAA CCCGGGCTGG CCTTCTAAGA AGAAGCTTGT TCGTAGGCTC CGAGAAAGTC 
TCTGACAGGA AGTTCCTGAC CCCTGAAGAT GAAGCCAGTA TTTTTACCAT GGACCACTTC 
CCGCTGTGGT ATCGCCAGGC CTCTGAGCAG CCCCCCGGCA GCTTTGTCTT CAATCTCCGC 
TGGGCAGAGG GACCAGATAG CCCAGGCAAG CCAGTGGCGG TAAGGGCCAG CACAGCAGTA 
ACTGTGACCG TGGATGGGAA GACGGCCATT GCAGCAGCTG TGGGCATCCA GATGCAAGCA 
GACTACCTCC AGCGCCAGTT CTGGGCAGCC ATGCAGCAGT GCAACGCTGT AGAGGGGCCG 
TGCCTGAAGA GCTGCGAGGA CACTGATCTC GACTGCTTCG TCATAGACAA CAATGGCTTC 
GTCCTGATCT CAGAGAGACC CCAAGAGATG GGAAGACTTC TGGGGGAAGC GGATGGTGCT 
CTCATGAAGC AACTTCTCAG CATGGGGGTG TTCAGCCGTG TGACCATGTA TGACTACCAG 
GCCATGTGCA AGCCCCCAGA TCATCACCAC AGTGCGGCCG AATCCCTGTT CAGTCCTCTC 
TCTGCCTTCT TGATGGTGGC CAGGTGGCTA CTGCATGAAT GTCTCCTATT CCTGTTAGAG 
TGGAGTGCCT GGGGATCTTG GCAGGACAAA GGGTCAGAGG CCAAATCTGT CTTCCATCAC 
TCCCACAAGC ACAAGAAGCA GGACCTGCTG CACCCCTGTG ACACAGAGTA CCCAGTGTTC 
GTGCATCAGA CGGCCATCCA GGAAGCCAAT GGCATCATCG AGTGTGGGGG CTGCCAGAAG 
ACATTCGTGA TGCAACAGAT TCCCCGAAGC AACCTGCTCC TGCTCGTGAC AGACCGCACC 
TGTGACTGCA GCGCCCACTC TCCCATCCTC CAGGAGGCCA CAGAAGTCAA ATATAATGCC 
TCTGTCAAGT GTGACAGGAT GCGCTCCCAG AAGCCCCGGA GACGACCAGG CTCCTGCCAT 
GCCTTCCATC CAGAGGAGAA TGCCCAGGAC TGTGGCGGTG CTTCAGACAC TCTGCCTTCA 
TCCCCTCTGC TCCTGCTATC ACTGGGGGCC TGGCTGCTTC CACCCCAGCT TCTGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Auxiliary Calcium Channel Subunit ?2?4 Is Required for Axonal Elaboration, Synaptic Transmission, and Wiring of Rod Photoreceptors.
Neuron93(6)1359-1374.e6(2017 Mar)
Wang Y,Fehlhaber KE,Sarria I,Cao Y,Ingram NT,Guerrero-Given D,Throesch B,Baldwin K,Kamasawa N,Ohtsuka T,Sampath AP,Martemyanov KA

A New Splicing Isoform of Cacna2d4 Mimicking the Effects of c.2451insC Mutation in the Retina: Novel Molecular and Electrophysiological Insights.
Investigative ophthalmology & visual science56(8)4846-56(2015 Jul)
Bacchi N,Messina A,Burtscher V,Dassi E,Provenzano G,Bozzi Y,Demontis GC,Koschak A,Denti MA,Casarosa S

Differential neuronal targeting of a new and two known calcium channel ?4 subunit splice variants correlates with their regulation of gene expression.
The Journal of neuroscience : the official journal of the Society for Neuroscience34(4)1446-61(2014 Jan)
Etemad S,Obermair GJ,Bindreither D,Benedetti A,Stanika R,Di Biase V,Burtscher V,Koschak A,Kofler R,Geley S,Wille A,Lusser A,Flockerzi V,Flucher BE

Voltage-activated calcium channel expression profiles in mouse brain and cultured hippocampal neurons.
Neuroscience167(3)786-98(2010 May)
Schlick B,Flucher BE,Obermair GJ

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Cacna2d4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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