Cngb3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cngb3
Entrez Gene ID	30952
Full Name	cyclic nucleotide gated channel beta 3
Synonyms	CCNC2,CNG6,Cngbeta2
General protein information	Preferred Names cyclic nucleotide gated channel beta 3 Names cyclic nucleotide-gated cation channel beta-3 CNG channel beta-3 cone photoreceptor cGMP-gated channel subunit beta cyclic nucleotide gated channel beta 6 cyclic nucleotide-gated cation channel modulatory subunit cyclic nucleotide-gated channel subunit CNG6
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_013927.2	NP_038955.1	cyclic nucleotide-gated cation channel beta-3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CNGB3	XP_519846.2
Homo sapiens (human)	CNGB3	NP_061971.3
	CNGB3	XP_001083646.1
Bos taurus (cattle)	CNGB3	XP_002692873.2
Danio rerio (zebrafish)	cngb3	XP_691142.4
Canis lupus familiaris (dog)	CNGB3	NP_001003030.1
Rattus norvegicus (Norway rat)	Cngb3	NP_001258167.1
Danio rerio (zebrafish)	LOC562466	XP_690937.4
Xenopus tropicalis (tropical clawed frog)	cngb3	XP_002939202.2
Mus musculus (house mouse)	Cngb3	NP_038955.1
Gallus gallus (chicken)	CNGB3	XP_425928.4

Gene Ontology
Bibliography

Related articles in PubMed

Inhibition of thyroid hormone receptor locally in the retina is a therapeutic strategy for retinal degeneration.
Ma H, Yang F, Butler MR, Belcher J, Redmond TM, Placzek AT, Scanlan TS, Ding XQ
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(8)3425-3438(2017 Aug)

Targeting iodothyronine deiodinases locally in the retina is a therapeutic strategy for retinal degeneration.
Yang F, Ma H, Belcher J, Butler MR, Redmond TM, Boye SL, Hauswirth WW, Ding XQ
FASEB journal : official publication of the Federation of American Societies for Experimental Biology30(12)4313-4325(2016 12)

The B3 Subunit of the Cone Cyclic Nucleotide-gated Channel Regulates the Light Responses of Cones and Contributes to the Channel Structural Flexibility.
Ding XQ, Thapa A, Ma H, Xu J, Elliott MH, Rodgers KK, Smith ML, Wang JS, Pittler SJ, Kefalov VJ
The Journal of biological chemistry291(16)8721-34(2016 Apr)

Intravitreal Ciliary Neurotrophic Factor Transiently Improves Cone-Mediated Function in a CNGB3-/- Mouse Model of Achromatopsia.
Marangoni D, Vijayasarathy C, Bush RA, Wei LL, Wen R, Sieving PA
Investigative ophthalmology & visual science56(11)6810-22(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CNGB3 regulates cone light response kinetics and the channel structural flexibility.
Title: The B3 Subunit of the Cone Cyclic Nucleotide-gated Channel Regulates the Light Responses of Cones and Contributes to the Channel Structural Flexibility.

This work investigated the functional modulation of cone CNG channel by exploring the channel-interacting proteins.
Title: Exploration of cone cyclic nucleotide-gated channel-interacting proteins using affinity purification and mass spectrometry.

Cone CNG channel is a heterotetrameric complex likely at a stoichiometry of three CNGA3 and one CNGB3.
Title: Biochemical characterization of cone cyclic nucleotide-gated (CNG) channel using the infrared fluorescence detection system.

RDS does not interact with the cone CNG.
Title: RDS in cones does not interact with the beta subunit of the cyclic nucleotide gated channel.

The heteromeric cyclic nucleotide-gated channel adopts a 3A:1B stoichiometry
Title: The heteromeric cyclic nucleotide-gated channel adopts a 3A:1B stoichiometry.

The following Cngb3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cngb3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu20015	NM_013927.2 Latest version!	Mus musculus cyclic nucleotide gated channel beta 3 (Cngb3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cngb3 ( NM_013927.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038955.1 Mus musculus cyclic nucleotide gated channel beta 3 (Cngb3), mRNA.

Price & Availability↑

CloneID	OMu20015
Clone ID Related Accession (Same CDS sequence)	NM_013927.2
Accession Version	NM_013927.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Mus musculus(house mouse)
Product	cyclic nucleotide-gated cation channel beta-3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX001053.7, AL772281.7, BX640400.4 and AL683894.6. On Nov 17, 2006 this sequence version replaced NM_013927.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ243572.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN01164138, SAMN01164141 [ECO:0000350] ##Evidence-Data-END##

ATGTTAAAAT CACTGACAGT AAAATTCAAC AAAGTGAATC CCATGGAAGG GAGAATGGAG 
AAGAAACTCT GCCCCAACCT GAGCAGTCTT TCTCAGCCAA CCATAGCACA GGGAGACAAC 
CAAAGTGAAA AAGAACCTCT GAGGAGCAGG ACACCAATCA CATTTGAAAA GTCACACAGC 
AAAGAAGACA ACAGCACTGG GGAAAACTCT CTCAGAGATT TCACCCCAAA TCCTGACCCT 
GAATGTCGAG CAGAACTCAC CAGAACAATG GCAGAGATGG AGAAAACTAG GACTGGGAAA 
GAAAGGCCAG TCAGCTTTAA GACCAAAGTG CTTGAAACCT CAATTATAAA TGAATATACT 
GATGCCCACC TACACAACCT AGTGGAAAGA ATGCGTGAAA GGACTGCTCT CTACAAGAAA 
ACATTGACAG AGGAAGAAAA TTTCCCAGAA GTGGAAGCCA GCTCTCAGAC TGCAATGTCT 
ACAAATATAT CACCAAAACA AGAGAACAAT TCCAAGCTAA AAGAACACCA AGACACGTTT 
TCTTTCAAAC CCCAGAGAGT CCCAGTAAAA GAGCACCTAC GAAGAATGAT ACTTCCCAGA 
AGCATAGACT CCTACACAGA TCGGGTCTAT CTCTTGTGGC TCCTGCTTGT CACCATTGCG 
TATAACTGGA ACTGTTGGCT GCTACCAGTG CGCCTCGTCT TTCCATGCCA AACACCAGAT 
AACAAGAACT ACTGGATTAT TACTGACATC GTATGTGATA TCATCTACCT TTGTGATATA 
CTATTGATCC AGCCAAGACT CCAATTTGTA AGAGGAGGAG AAATTATTGT AGATTCAAAT 
GAGCTGAAGA GGAACTACCG GAGTTCTACA AAGTTTCGGA TGGATGTGGC ATCTCTACTG 
CCATTCGAAG TTCTCTACAT CTTCTTTGGA GTTAATCCAA TATTCAGAGC AAATCGGATA 
TTAAAGTATA CTTCATTCTT TGAGTTTAAC CATCACCTGG AGTCTATAAT GGACAAAGCA 
TATGTCTACA GAGTCATCCG AACAACTGGC TACTTGCTGT TTCTCCTGCA CATTAACGCC 
TGTGTTTATT ACTGGGCTTC AGACTATGAA GGAATTGGCT CAACTAAATG GGTCTATAAT 
GGTGAAGGCA ACAAGTATCT GCGATGCTTT TATTGGGCAG TTCGAACTTT AATTACTATC 
GGGGGCCTTC CAGAGCCACA GACTTCATTT GAAATTGTTT TTCAATTCTT GAATTTTTTC 
TCTGGGGTTT TTGTTTTCTC CAGCTTAATT GGTCAGATGC GTGATGTGAT TGGGGCAGCA 
ACAGCCAATC AGAACTACTT CCAAGCCTGC ATGGACCATA TCATTGCCTA CATGAACAAA 
TACTCTATTC CTCAGAGTGT GCAGTATCGA GTTCGGACTT GGCTGGAATA TACATGGAAC 
TCACAAAGAA TCCTAGATGA GTCCAACTTG CTTGAGAACC TCCCGACAGC AATGCAGTTG 
TCTATTGCCC TTGACATAAA CTTCAGTATC ATCGACAAGG TGGAGTTATT CAAGGGCTGT 
GACACACAGA TGATTTATGA CCTGCTGCTA AGATTGAAAT CCACTATTTA TTTACCTGGT 
GACTTTGTCT GCAAAAAGGG AGAAATTGGA AAAGAAATGT ACATCATCAA ACACGGAGAA 
GTCCAAGTCC TTGGAGGCCC TGATGGTGCT CAAGTTCTGG TTACTCTGAA AGCTGGGTCA 
GTGTTTGGAG AAATAAGCCT TCTGGCAAAA GGAGGAGGAA ATCGCCGGAC AGCTGATGTT 
GTGGCCCACG GATTTGCCAA TCTTTTAACT CTGGACAAAA AGACTCTCCA AGAAATTCTG 
CTGCATTATC CAACTTCTAA AAAGCTCCTC ATGAAGAAAG CCAAAATTCT TTTAAGCCAG 
AAGGGAAAGA CCACTCAGGC AATCCCTGCA AGACCAGGAC CTGCCTTTCT TTTCCCACCA 
AAAGAAGAGA CACCCCGAAT GCTTAAAGTT CTCCTAGGAA ACACAGGAAA GGTGGACCTT 
GGAAGACTCC TTAAAGGAAA GAGAAAAACA ACCACTCAGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038955.1
CDS	84..2168
Translation	MLKSLTVKFNKVNPMEGRMEKKLCPNLSSLSQPTIAQGDNQSEKEPLRSRTPITFEKSHSKEDNSTGENSLRDFTPNPDPECRAELTRTMAEMEKTRTGKERPVSFKTKVLETSIINEYTDAHLHNLVERMRERTALYKKTLTEEENFPEVEASSQTAMSTNISPKQENNSKLKEHQDTFSFKPQRVPVKEHLRRMILPRSIDSYTDRVYLLWLLLVTIAYNWNCWLLPVRLVFPCQTPDNKNYWIITDIVCDIIYLCDILLIQPRLQFVRGGEIIVDSNELKRNYRSSTKFRMDVASLLPFEVLYIFFGVNPIFRANRILKYTSFFEFNHHLESIMDKAYVYRVIRTTGYLLFLLHINACVYYWASDYEGIGSTKWVYNGEGNKYLRCFYWAVRTLITIGGLPEPQTSFEIVFQFLNFFSGVFVFSSLIGQMRDVIGAATANQNYFQACMDHIIAYMNKYSIPQSVQYRVRTWLEYTWNSQRILDESNLLENLPTAMQLSIALDINFSIIDKVELFKGCDTQMIYDLLLRLKSTIYLPGDFVCKKGEIGKEMYIIKHGEVQVLGGPDGAQVLVTLKAGSVFGEISLLAKGGGNRRTADVVAHGFANLLTLDKKTLQEILLHYPTSKKLLMKKAKILLSQKGKTTQAIPARPGPAFLFPPKEETPRMLKVLLGNTGKVDLGRLLKGKRKTTTQK

Target ORF information:

RefSeq Version	NM_013927.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cyclic nucleotide gated channel beta 3 (Cngb3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013927.2

ORF Insert Sequence:

ATGTTAAAAT CACTGACAGT AAAATTCAAC AAAGTGAATC CCATGGAAGG GAGAATGGAG 
AAGAAACTCT GCCCCAACCT GAGCAGTCTT TCTCAGCCAA CCATAGCACA GGGAGACAAC 
CAAAGTGAAA AAGAACCTCT GAGGAGCAGG ACACCAATCA CATTTGAAAA GTCACACAGC 
AAAGAAGACA ACAGCACTGG GGAAAACTCT CTCAGAGATT TCACCCCAAA TCCTGACCCT 
GAATGTCGAG CAGAACTCAC CAGAACAATG GCAGAGATGG AGAAAACTAG GACTGGGAAA 
GAAAGGCCAG TCAGCTTTAA GACCAAAGTG CTTGAAACCT CAATTATAAA TGAATATACT 
GATGCCCACC TACACAACCT AGTGGAAAGA ATGCGTGAAA GGACTGCTCT CTACAAGAAA 
ACATTGACAG AGGAAGAAAA TTTCCCAGAA GTGGAAGCCA GCTCTCAGAC TGCAATGTCT 
ACAAATATAT CACCAAAACA AGAGAACAAT TCCAAGCTAA AAGAACACCA AGACACGTTT 
TCTTTCAAAC CCCAGAGAGT CCCAGTAAAA GAGCACCTAC GAAGAATGAT ACTTCCCAGA 
AGCATAGACT CCTACACAGA TCGGGTCTAT CTCTTGTGGC TCCTGCTTGT CACCATTGCG 
TATAACTGGA ACTGTTGGCT GCTACCAGTG CGCCTCGTCT TTCCATGCCA AACACCAGAT 
AACAAGAACT ACTGGATTAT TACTGACATC GTATGTGATA TCATCTACCT TTGTGATATA 
CTATTGATCC AGCCAAGACT CCAATTTGTA AGAGGAGGAG AAATTATTGT AGATTCAAAT 
GAGCTGAAGA GGAACTACCG GAGTTCTACA AAGTTTCGGA TGGATGTGGC ATCTCTACTG 
CCATTCGAAG TTCTCTACAT CTTCTTTGGA GTTAATCCAA TATTCAGAGC AAATCGGATA 
TTAAAGTATA CTTCATTCTT TGAGTTTAAC CATCACCTGG AGTCTATAAT GGACAAAGCA 
TATGTCTACA GAGTCATCCG AACAACTGGC TACTTGCTGT TTCTCCTGCA CATTAACGCC 
TGTGTTTATT ACTGGGCTTC AGACTATGAA GGAATTGGCT CAACTAAATG GGTCTATAAT 
GGTGAAGGCA ACAAGTATCT GCGATGCTTT TATTGGGCAG TTCGAACTTT AATTACTATC 
GGGGGCCTTC CAGAGCCACA GACTTCATTT GAAATTGTTT TTCAATTCTT GAATTTTTTC 
TCTGGGGTTT TTGTTTTCTC CAGCTTAATT GGTCAGATGC GTGATGTGAT TGGGGCAGCA 
ACAGCCAATC AGAACTACTT CCAAGCCTGC ATGGACCATA TCATTGCCTA CATGAACAAA 
TACTCTATTC CTCAGAGTGT GCAGTATCGA GTTCGGACTT GGCTGGAATA TACATGGAAC 
TCACAAAGAA TCCTAGATGA GTCCAACTTG CTTGAGAACC TCCCGACAGC AATGCAGTTG 
TCTATTGCCC TTGACATAAA CTTCAGTATC ATCGACAAGG TGGAGTTATT CAAGGGCTGT 
GACACACAGA TGATTTATGA CCTGCTGCTA AGATTGAAAT CCACTATTTA TTTACCTGGT 
GACTTTGTCT GCAAAAAGGG AGAAATTGGA AAAGAAATGT ACATCATCAA ACACGGAGAA 
GTCCAAGTCC TTGGAGGCCC TGATGGTGCT CAAGTTCTGG TTACTCTGAA AGCTGGGTCA 
GTGTTTGGAG AAATAAGCCT TCTGGCAAAA GGAGGAGGAA ATCGCCGGAC AGCTGATGTT 
GTGGCCCACG GATTTGCCAA TCTTTTAACT CTGGACAAAA AGACTCTCCA AGAAATTCTG 
CTGCATTATC CAACTTCTAA AAAGCTCCTC ATGAAGAAAG CCAAAATTCT TTTAAGCCAG 
AAGGGAAAGA CCACTCAGGC AATCCCTGCA AGACCAGGAC CTGCCTTTCT TTTCCCACCA 
AAAGAAGAGA CACCCCGAAT GCTTAAAGTT CTCCTAGGAA ACACAGGAAA GGTGGACCTT 
GGAAGACTCC TTAAAGGAAA GAGAAAAACA ACCACTCAGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inhibition of thyroid hormone receptor locally in the retina is a therapeutic strategy for retinal degeneration.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(8)3425-3438(2017 Aug)
Ma H,Yang F,Butler MR,Belcher J,Redmond TM,Placzek AT,Scanlan TS,Ding XQ

Targeting iodothyronine deiodinases locally in the retina is a therapeutic strategy for retinal degeneration.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology30(12)4313-4325(2016 12)
Yang F,Ma H,Belcher J,Butler MR,Redmond TM,Boye SL,Hauswirth WW,Ding XQ

The B3 Subunit of the Cone Cyclic Nucleotide-gated Channel Regulates the Light Responses of Cones and Contributes to the Channel Structural Flexibility.
The Journal of biological chemistry291(16)8721-34(2016 Apr)
Ding XQ,Thapa A,Ma H,Xu J,Elliott MH,Rodgers KK,Smith ML,Wang JS,Pittler SJ,Kefalov VJ

Intravitreal Ciliary Neurotrophic Factor Transiently Improves Cone-Mediated Function in a CNGB3-/- Mouse Model of Achromatopsia.
Investigative ophthalmology & visual science56(11)6810-22(2015 Oct)
Marangoni D,Vijayasarathy C,Bush RA,Wei LL,Wen R,Sieving PA