Dbf4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dbf4
Entrez Gene ID	27214
Full Name	DBF4 zinc finger
Synonyms	AA545217,Ask
General protein information	Preferred Names DBF4 zinc finger Names protein DBF4 homolog A DBF4 homolog activator of S phase kinase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001190717.1	NP_001177646.1	protein DBF4 homolog A isoform 2
NM_013726.3	NP_038754.1	protein DBF4 homolog A isoform 1
XM_011240670.2	XP_011238972.1	protein DBF4 homolog A isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DBF4	NP_001068944.2
Rattus norvegicus (Norway rat)	Dbf4	NP_001178677.1
Rattus norvegicus (Norway rat)	LOC100912278	XP_003749729.1
Pan troglodytes (chimpanzee)	DBF4	XP_528041.3
	DBF4	XP_001104753.2
Gallus gallus (chicken)	DBF4	XP_004939326.1
Mus musculus (house mouse)	Dbf4	NP_001177646.1
Homo sapiens (human)	DBF4	NP_006707.1
Canis lupus familiaris (dog)	DBF4	XP_532451.2

Gene Ontology
Bibliography

Related articles in PubMed

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
Sundberg JP, Dadras SS, Silva KA, Kennedy VE, Garland G, Murray SA, Sundberg BA, Schofield PN, Pratt CH
PloS one12(7)e0180682(2017)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst.
Guo G, Huss M, Tong GQ, Wang C, Li Sun L, Clarke ND, Robson P
Developmental cell18(4)675-85(2010 Apr)

Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells.
Kneissl M, P?tter V, Szalay AA, Grummt F
Journal of molecular biology327(1)111-28(2003 Mar)

Expression of genes involved in mammalian meiosis during the transition from egg to embryo.
Hwang SY, Oh B, Knowles BB, Solter D, Lee JS
Molecular reproduction and development59(2)144-58(2001 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dbf4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dbf4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu01344	NM_001190717.1 Latest version!	Mus musculus DBF4 zinc finger (Dbf4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu09847	NM_013726.3 Latest version!	Mus musculus DBF4 zinc finger (Dbf4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu69273	XM_011240670.2 Latest version!	Mus musculus DBF4 zinc finger (Dbf4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dbf4 ( NM_001190717.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001177646.1 Mus musculus DBF4 zinc finger (Dbf4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu01344
Clone ID Related Accession (Same CDS sequence)	NM_001190717.1
Accession Version	NM_001190717.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	protein DBF4 homolog A isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB611145.1, AK132403.1 and AC152165.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK132403.1, BC145392.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END##

ATGAACCTCG AGACCATGAG GATCCACAGC AAAGCACCTC TCCCGGGTGG CATCCAAGAC 
AGAAATGAAA AGAATCGACC ATCCTTGAAA TCCCTGAAGG CTGATAATAG ACTGGAAAAA 
TCCAAATATA AGCCCCTTTG GGGAAAAATA TTTTACCTTG ACTTGCCTTC TATCACCATA 
TGTGAGAAAC TACAAAAAGA TATAAAGGAA CTCGGAGGGC GAGTTGAAGA ATTTCTCAGC 
AAAGATATCA GTTATTTTGT TTCAAATAAA AAGGAAGCAA AGTATGCACA GACATTGGGT 
CGCGTTTCTC CTGTACCAAG TCCAGAATCT GCATATACTG CAGAAACCAC CTCTCCTCAT 
CCCAGCCATG ATGGAAGTTC CTTTAAGTCT CAAGACAGAG TATGTTTGAG CCGAGGAAAA 
TTGTTAGCTG AAAAAGCTGT GAAGGATCAT GATTTTATTC CTGCTAATAG TATATTATCA 
AATGCCTTAT CATGGGGAGT AAAGATTCTT CATATTGATG ACATTCGATA CTACATTGAA 
CAGAAGAAGA AAGCACTGAG TGCACTCAAG AAATCCAGTG CTTCTGGGAA GGATGCGGGG 
AAAAAAGCTG GTCCTGGAAT ACAAAAGACA AGAAGGAGAC TCAAGAAGCC TTTTTTAAAG 
GTTGAAGATG TGAACCGGTG CTACAGGCCA TTTTACCTTC AGCTCCCCAG TCTGCCGTGT 
ATAAACTACT TTCTTCAGAA GCCCTGCAGT CCGTTTGATA TAGAGAAGTC ATCCAGCGTC 
CAGAAACAAG CTCAGCCCAA GCTCAGAATT AATATGGACG GCGATAAATG TGGAACCCCA 
GTTCAACTCC AGTTGAAGGA AAAGAGAAAA AAAGGATATT GTGAATGTTG CTTACAGAAA 
TACGAAGATC TCGAAACTCA CCTTCTGAGT GAGAAACACA GGAACTTTGC ACAGAGTAAC 
CAGTATCAAG TTGTTGATGA TATTGTATCT CAGTTAGTTT TCGATTTTGT GGAATATGGA 
AGAGACACAC CTCAAAAGAA AAGAATAAGA TACAGTGTCG GGTCCCTTTC TTCTGTTTCT 
GCAAATGTCC TGAAAAACAC TGCACCGAAG GAGAAGCCAC TCTTGGAGCC CAATTTCCAG 
AAGGACGTGG GCGAAAGCAG TGGGCACCTG CTCAAGCCGA ACTCTCAGTA TGAAGAAACC 
CAGAAACCTG AAGAAAAGCA TGGGTTTGCT TCAGAGCCCA CAACCTATTC TTCAGCTGGA 
TTGAAAGGAT GTGACAGGAA ACCAGTGAGC ATGTTCAATG CAAGTGAGCC TGACCCAGAG 
CAGGAGTATG CACAGCTACC TCTGCGTGAC AGCACACCGG AACACCAAGT GACCGAGGGT 
AGAAATGACG GAGAACAGAG AGTAGACCCT GCTCCAGGCG TGTCGCAGTC ATGTGGACAA 
GTTTCTCATC TCAGTACAGA AAGTAATCTA CCCCAACCAC AGTTAGCTGC AGACATTACA 
CAGCTTTCAG CAAAAGATCT GCAAGAAAAG GGCTTTCATG TTGTGATAGG TCATGCTTCT 
GACCTGGTGG CATTAAATAC ATCAAAAGAG CAGCTAACAA TGAAGGCGAG AACTCCACCC 
TGTAGTCCCC AGGAGCCTCA TGAGTGTGAC ACTGAGAACA TGGAGAATTT GCCTTGTGGT 
AAAATCCAGC GGAAAGTGAG AATGCTATTA GGGCAACAGA AAGCAAATGC TGAACCGAGT 
GCTGAATTGG ATAAAAAGAG AACTGAGTAT CTTCCTGCAC ATGAAGACAG AACTTGTGGG 
TCACCAGTAC AGTCTCTATT GGACCTTTTT CAGACAAGCG AGGAGAAATC AGAATTTTTG 
GGTTTTACAG GCTACACTGA AAACAGTGGA ATATGTGATG TTTTAGATAT TTGGGAGGAA 
GAAAATTCAA GTACCCTGTT GTCAACGTTT TTTTCTTCCC CTTCAACTTC TGCATTCGTT 
GGATTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177646.1
CDS	205..2193
Translation	MNLETMRIHSKAPLPGGIQDRNEKNRPSLKSLKADNRLEKSKYKPLWGKIFYLDLPSITICEKLQKDIKELGGRVEEFLSKDISYFVSNKKEAKYAQTLGRVSPVPSPESAYTAETTSPHPSHDGSSFKSQDRVCLSRGKLLAEKAVKDHDFIPANSILSNALSWGVKILHIDDIRYYIEQKKKALSALKKSSASGKDAGKKAGPGIQKTRRRLKKPFLKVEDVNRCYRPFYLQLPSLPCINYFLQKPCSPFDIEKSSSVQKQAQPKLRINMDGDKCGTPVQLQLKEKRKKGYCECCLQKYEDLETHLLSEKHRNFAQSNQYQVVDDIVSQLVFDFVEYGRDTPQKKRIRYSVGSLSSVSANVLKNTAPKEKPLLEPNFQKDVGESSGHLLKPNSQYEETQKPEEKHGFASEPTTYSSAGLKGCDRKPVSMFNASEPDPEQEYAQLPLRDSTPEHQVTEGRNDGEQRVDPAPGVSQSCGQVSHLSTESNLPQPQLAADITQLSAKDLQEKGFHVVIGHASDLVALNTSKEQLTMKARTPPCSPQEPHECDTENMENLPCGKIQRKVRMLLGQQKANAEPSAELDKKRTEYLPAHEDRTCGSPVQSLLDLFQTSEEKSEFLGFTGYTENSGICDVLDIWEEENSSTLLSTFFSSPSTSAFVGF

Target ORF information:

RefSeq Version	NM_001190717.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DBF4 zinc finger (Dbf4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190717.1

ORF Insert Sequence:

ATGAACCTCG AGACCATGAG GATCCACAGC AAAGCACCTC TCCCGGGTGG CATCCAAGAC 
AGAAATGAAA AGAATCGACC ATCCTTGAAA TCCCTGAAGG CTGATAATAG ACTGGAAAAA 
TCCAAATATA AGCCCCTTTG GGGAAAAATA TTTTACCTTG ACTTGCCTTC TATCACCATA 
TGTGAGAAAC TACAAAAAGA TATAAAGGAA CTCGGAGGGC GAGTTGAAGA ATTTCTCAGC 
AAAGATATCA GTTATTTTGT TTCAAATAAA AAGGAAGCAA AGTATGCACA GACATTGGGT 
CGCGTTTCTC CTGTACCAAG TCCAGAATCT GCATATACTG CAGAAACCAC CTCTCCTCAT 
CCCAGCCATG ATGGAAGTTC CTTTAAGTCT CAAGACAGAG TATGTTTGAG CCGAGGAAAA 
TTGTTAGCTG AAAAAGCTGT GAAGGATCAT GATTTTATTC CTGCTAATAG TATATTATCA 
AATGCCTTAT CATGGGGAGT AAAGATTCTT CATATTGATG ACATTCGATA CTACATTGAA 
CAGAAGAAGA AAGCACTGAG TGCACTCAAG AAATCCAGTG CTTCTGGGAA GGATGCGGGG 
AAAAAAGCTG GTCCTGGAAT ACAAAAGACA AGAAGGAGAC TCAAGAAGCC TTTTTTAAAG 
GTTGAAGATG TGAACCGGTG CTACAGGCCA TTTTACCTTC AGCTCCCCAG TCTGCCGTGT 
ATAAACTACT TTCTTCAGAA GCCCTGCAGT CCGTTTGATA TAGAGAAGTC ATCCAGCGTC 
CAGAAACAAG CTCAGCCCAA GCTCAGAATT AATATGGACG GCGATAAATG TGGAACCCCA 
GTTCAACTCC AGTTGAAGGA AAAGAGAAAA AAAGGATATT GTGAATGTTG CTTACAGAAA 
TACGAAGATC TCGAAACTCA CCTTCTGAGT GAGAAACACA GGAACTTTGC ACAGAGTAAC 
CAGTATCAAG TTGTTGATGA TATTGTATCT CAGTTAGTTT TCGATTTTGT GGAATATGGA 
AGAGACACAC CTCAAAAGAA AAGAATAAGA TACAGTGTCG GGTCCCTTTC TTCTGTTTCT 
GCAAATGTCC TGAAAAACAC TGCACCGAAG GAGAAGCCAC TCTTGGAGCC CAATTTCCAG 
AAGGACGTGG GCGAAAGCAG TGGGCACCTG CTCAAGCCGA ACTCTCAGTA TGAAGAAACC 
CAGAAACCTG AAGAAAAGCA TGGGTTTGCT TCAGAGCCCA CAACCTATTC TTCAGCTGGA 
TTGAAAGGAT GTGACAGGAA ACCAGTGAGC ATGTTCAATG CAAGTGAGCC TGACCCAGAG 
CAGGAGTATG CACAGCTACC TCTGCGTGAC AGCACACCGG AACACCAAGT GACCGAGGGT 
AGAAATGACG GAGAACAGAG AGTAGACCCT GCTCCAGGCG TGTCGCAGTC ATGTGGACAA 
GTTTCTCATC TCAGTACAGA AAGTAATCTA CCCCAACCAC AGTTAGCTGC AGACATTACA 
CAGCTTTCAG CAAAAGATCT GCAAGAAAAG GGCTTTCATG TTGTGATAGG TCATGCTTCT 
GACCTGGTGG CATTAAATAC ATCAAAAGAG CAGCTAACAA TGAAGGCGAG AACTCCACCC 
TGTAGTCCCC AGGAGCCTCA TGAGTGTGAC ACTGAGAACA TGGAGAATTT GCCTTGTGGT 
AAAATCCAGC GGAAAGTGAG AATGCTATTA GGGCAACAGA AAGCAAATGC TGAACCGAGT 
GCTGAATTGG ATAAAAAGAG AACTGAGTAT CTTCCTGCAC ATGAAGACAG AACTTGTGGG 
TCACCAGTAC AGTCTCTATT GGACCTTTTT CAGACAAGCG AGGAGAAATC AGAATTTTTG 
GGTTTTACAG GCTACACTGA AAACAGTGGA ATATGTGATG TTTTAGATAT TTGGGAGGAA 
GAAAATTCAA GTACCCTGTT GTCAACGTTT TTTTCTTCCC CTTCAACTTC TGCATTCGTT 
GGATTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dbf4 ( NM_013726.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038754.1 Mus musculus DBF4 zinc finger (Dbf4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu09847
Clone ID Related Accession (Same CDS sequence)	NM_013726.3
Accession Version	NM_013726.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	protein DBF4 homolog A isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB611145.1, BC138709.1, AK132403.1 and AC152165.2. On Jul 7, 2010 this sequence version replaced NM_013726.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1660815.245740.1, AJ003132.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END##

ATGAACCTCG AGACCATGAG GATCCACAGC AAAGCACCTC TCCCGGGTGG CATCCAAGAC 
AGAAATGAAA AGAATCGACC ATCCTTGAAA TCCCTGAAGG CTGATAATAG ACTGGAAAAA 
TCCAAATATA AGCCCCTTTG GGGAAAAATA TTTTACCTTG ACTTGCCTTC TATCACCATA 
TGTGAGAAAC TACAAAAAGA TATAAAGGAA CTCGGAGGGC GAGTTGAAGA ATTTCTCAGC 
AAAGATATCA GTTATTTTGT TTCAAATAAA AAGGAAGCAA AGTATGCACA GACATTGGGT 
CGCGTTTCTC CTGTACCAAG TCCAGAATCT GCATATACTG CAGAAACCAC CTCTCCTCAT 
CCCAGCCATG ATGGAAGTTC CTTTAAGTCT CAAGACAGAG TATGTTTGAG CCGAGGAAAA 
TTGTTAGCTG AAAAAGCTGT GAAGGATCAT GATTTTATTC CTGCTAATAG TATATTATCA 
AATGCCTTAT CATGGGGAGT AAAGATTCTT CATATTGATG ACATTCGATA CTACATTGAA 
CAGAAGAAGA AAGCACTGAG TGCACTCAAG AAATCCAGTG CTTCTGGGAA GGATGCGGGG 
AAAAAAGCTG GTCCTGGAAT ACAAAAGACA AGAACAGGGA GACTCAAGAA GCCTTTTTTA 
AAGGTTGAAG ATGTGAACCG GTGCTACAGG CCATTTTACC TTCAGCTCCC CAGTCTGCCG 
TGTATAAACT ACTTTCTTCA GAAGCCCTGC AGTCCGTTTG ATATAGAGAA GTCATCCAGC 
GTCCAGAAAC AAGCTCAGCC CAAGCTCAGA ATTAATATGG ACGGCGATAA ATGTGGAACC 
CCAGTTCAAC TCCAGTTGAA GGAAAAGAGA AAAAAAGGAT ATTGTGAATG TTGCTTACAG 
AAATACGAAG ATCTCGAAAC TCACCTTCTG AGTGAGAAAC ACAGGAACTT TGCACAGAGT 
AACCAGTATC AAGTTGTTGA TGATATTGTA TCTCAGTTAG TTTTCGATTT TGTGGAATAT 
GGAAGAGACA CACCTCAAAA GAAAAGAATA AGATACAGTG TCGGGTCCCT TTCTTCTGTT 
TCTGCAAATG TCCTGAAAAA CACTGCACCG AAGGAGAAGC CACTCTTGGA GCCCAATTTC 
CAGAAGGACG TGGGCGAAAG CAGTGGGCAC CTGCTCAAGC CGAACTCTCA GTATGAAGAA 
ACCCAGAAAC CTGAAGAAAA GCATGGGTTT GCTTCAGAGC CCACAACCTA TTCTTCAGCT 
GGATTGAAAG GATGTGACAG GAAACCAGTG AGCATGTTCA ATGCAAGTGA GCCTGACCCA 
GAGCAGGAGT ATGCACAGCT ACCTCTGCGT GACAGCACAC CGGAACACCA AGTGACCGAG 
GGTAGAAATG ACGGAGAACA GAGAGTAGAC CCTGCTCCAG GCGTGTCGCA GTCATGTGGA 
CAAGTTTCTC ATCTCAGTAC AGAAAGTAAT CTACCCCAAC CACAGTTAGC TGCAGACATT 
ACACAGCTTT CAGCAAAAGA TCTGCAAGAA AAGGGCTTTC ATGTTGTGAT AGGTCATGCT 
TCTGACCTGG TGGCATTAAA TACATCAAAA GAGCAGCTAA CAATGAAGGC GAGAACTCCA 
CCCTGTAGTC CCCAGGAGCC TCATGAGTGT GACACTGAGA ACATGGAGAA TTTGCCTTGT 
GGTAAAATCC AGCGGAAAGT GAGAATGCTA TTAGGGCAAC AGAAAGCAAA TGCTGAACCG 
AGTGCTGAAT TGGATAAAAA GAGAACTGAG TATCTTCCTG CACATGAAGA CAGAACTTGT 
GGGTCACCAG TACAGTCTCT ATTGGACCTT TTTCAGACAA GCGAGGAGAA ATCAGAATTT 
TTGGGTTTTA CAGGCTACAC TGAAAACAGT GGAATATGTG ATGTTTTAGA TATTTGGGAG 
GAAGAAAATT CAAGTACCCT GTTGTCAACG TTTTTTTCTT CCCCTTCAAC TTCTGCATTC 
GTTGGATTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038754.1
CDS	205..2196
Translation	MNLETMRIHSKAPLPGGIQDRNEKNRPSLKSLKADNRLEKSKYKPLWGKIFYLDLPSITICEKLQKDIKELGGRVEEFLSKDISYFVSNKKEAKYAQTLGRVSPVPSPESAYTAETTSPHPSHDGSSFKSQDRVCLSRGKLLAEKAVKDHDFIPANSILSNALSWGVKILHIDDIRYYIEQKKKALSALKKSSASGKDAGKKAGPGIQKTRTGRLKKPFLKVEDVNRCYRPFYLQLPSLPCINYFLQKPCSPFDIEKSSSVQKQAQPKLRINMDGDKCGTPVQLQLKEKRKKGYCECCLQKYEDLETHLLSEKHRNFAQSNQYQVVDDIVSQLVFDFVEYGRDTPQKKRIRYSVGSLSSVSANVLKNTAPKEKPLLEPNFQKDVGESSGHLLKPNSQYEETQKPEEKHGFASEPTTYSSAGLKGCDRKPVSMFNASEPDPEQEYAQLPLRDSTPEHQVTEGRNDGEQRVDPAPGVSQSCGQVSHLSTESNLPQPQLAADITQLSAKDLQEKGFHVVIGHASDLVALNTSKEQLTMKARTPPCSPQEPHECDTENMENLPCGKIQRKVRMLLGQQKANAEPSAELDKKRTEYLPAHEDRTCGSPVQSLLDLFQTSEEKSEFLGFTGYTENSGICDVLDIWEEENSSTLLSTFFSSPSTSAFVGF

Target ORF information:

RefSeq Version	NM_013726.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DBF4 zinc finger (Dbf4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013726.3

ORF Insert Sequence:

ATGAACCTCG AGACCATGAG GATCCACAGC AAAGCACCTC TCCCGGGTGG CATCCAAGAC 
AGAAATGAAA AGAATCGACC ATCCTTGAAA TCCCTGAAGG CTGATAATAG ACTGGAAAAA 
TCCAAATATA AGCCCCTTTG GGGAAAAATA TTTTACCTTG ACTTGCCTTC TATCACCATA 
TGTGAGAAAC TACAAAAAGA TATAAAGGAA CTCGGAGGGC GAGTTGAAGA ATTTCTCAGC 
AAAGATATCA GTTATTTTGT TTCAAATAAA AAGGAAGCAA AGTATGCACA GACATTGGGT 
CGCGTTTCTC CTGTACCAAG TCCAGAATCT GCATATACTG CAGAAACCAC CTCTCCTCAT 
CCCAGCCATG ATGGAAGTTC CTTTAAGTCT CAAGACAGAG TATGTTTGAG CCGAGGAAAA 
TTGTTAGCTG AAAAAGCTGT GAAGGATCAT GATTTTATTC CTGCTAATAG TATATTATCA 
AATGCCTTAT CATGGGGAGT AAAGATTCTT CATATTGATG ACATTCGATA CTACATTGAA 
CAGAAGAAGA AAGCACTGAG TGCACTCAAG AAATCCAGTG CTTCTGGGAA GGATGCGGGG 
AAAAAAGCTG GTCCTGGAAT ACAAAAGACA AGAACAGGGA GACTCAAGAA GCCTTTTTTA 
AAGGTTGAAG ATGTGAACCG GTGCTACAGG CCATTTTACC TTCAGCTCCC CAGTCTGCCG 
TGTATAAACT ACTTTCTTCA GAAGCCCTGC AGTCCGTTTG ATATAGAGAA GTCATCCAGC 
GTCCAGAAAC AAGCTCAGCC CAAGCTCAGA ATTAATATGG ACGGCGATAA ATGTGGAACC 
CCAGTTCAAC TCCAGTTGAA GGAAAAGAGA AAAAAAGGAT ATTGTGAATG TTGCTTACAG 
AAATACGAAG ATCTCGAAAC TCACCTTCTG AGTGAGAAAC ACAGGAACTT TGCACAGAGT 
AACCAGTATC AAGTTGTTGA TGATATTGTA TCTCAGTTAG TTTTCGATTT TGTGGAATAT 
GGAAGAGACA CACCTCAAAA GAAAAGAATA AGATACAGTG TCGGGTCCCT TTCTTCTGTT 
TCTGCAAATG TCCTGAAAAA CACTGCACCG AAGGAGAAGC CACTCTTGGA GCCCAATTTC 
CAGAAGGACG TGGGCGAAAG CAGTGGGCAC CTGCTCAAGC CGAACTCTCA GTATGAAGAA 
ACCCAGAAAC CTGAAGAAAA GCATGGGTTT GCTTCAGAGC CCACAACCTA TTCTTCAGCT 
GGATTGAAAG GATGTGACAG GAAACCAGTG AGCATGTTCA ATGCAAGTGA GCCTGACCCA 
GAGCAGGAGT ATGCACAGCT ACCTCTGCGT GACAGCACAC CGGAACACCA AGTGACCGAG 
GGTAGAAATG ACGGAGAACA GAGAGTAGAC CCTGCTCCAG GCGTGTCGCA GTCATGTGGA 
CAAGTTTCTC ATCTCAGTAC AGAAAGTAAT CTACCCCAAC CACAGTTAGC TGCAGACATT 
ACACAGCTTT CAGCAAAAGA TCTGCAAGAA AAGGGCTTTC ATGTTGTGAT AGGTCATGCT 
TCTGACCTGG TGGCATTAAA TACATCAAAA GAGCAGCTAA CAATGAAGGC GAGAACTCCA 
CCCTGTAGTC CCCAGGAGCC TCATGAGTGT GACACTGAGA ACATGGAGAA TTTGCCTTGT 
GGTAAAATCC AGCGGAAAGT GAGAATGCTA TTAGGGCAAC AGAAAGCAAA TGCTGAACCG 
AGTGCTGAAT TGGATAAAAA GAGAACTGAG TATCTTCCTG CACATGAAGA CAGAACTTGT 
GGGTCACCAG TACAGTCTCT ATTGGACCTT TTTCAGACAA GCGAGGAGAA ATCAGAATTT 
TTGGGTTTTA CAGGCTACAC TGAAAACAGT GGAATATGTG ATGTTTTAGA TATTTGGGAG 
GAAGAAAATT CAAGTACCCT GTTGTCAACG TTTTTTTCTT CCCCTTCAAC TTCTGCATTC 
GTTGGATTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dbf4 ( XM_011240670.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238972.1 Mus musculus DBF4 zinc finger (Dbf4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu69273
Clone ID Related Accession (Same CDS sequence)	XM_011240670.2
Accession Version	XM_011240670.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein DBF4 homolog A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011240670.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCTCG AGACCATGAG GATCCACAGC AAAGCACCTC TCCCGGGTGG CATCCAAGAC 
AGAAATGAAA AGAATCGACC ATCCTTGAAA TCCCTGAAGG CTGATAATAG ACTGGAAAAA 
TCCAAATATA AGCCCCTTTG GGGAAAAATA TTTTACCTTG ACTTGCCTTC TATCACCATA 
TGTGAGAAAC TACAAAAAGA TATAAAGGAA CTCGGAGGGC GAGTTGAAGA ATTTCTCAGC 
AAAGATATCA GTTATTTTGT TTCAAATAAA AAGGAAGCAA AGTATGCACA GACATTGGGT 
CGCGTTTCTC CTGTACCAAG TCCAGAATCT GCATATACTG CAGAAACCAC CTCTCCTCAT 
CCCAGCCATG ATGGAAGTTC CTTTAAGTCT CAAGACAGAG TATGTTTGAG CCGAGGAAAA 
TTGTTAGCTG AAAAAGCTGT GAAGGATCAT GATTTTATTC CTGCTAATAG TATATTATCA 
AATGCCTTAT CATGGGGAGT AAAGATTCTT CATATTGATG ACATTCGATA CTACATTGAA 
CAGAAGAAGA AAGCACTGAG TGCACTCAAG AAATCCAGTG CTTCTGGGAA GGATGCGGGG 
AAAAAAGCTG GTCCTGGAAT ACAAAAGACA AGAACAGGGA GACTCAAGAA GCCTTTTTTA 
AAGGTTGAAG ATGTGAACCG GTGCTACAGG CCATTTTACC TTCAGCTCCC CAGTCTGCCG 
TGTATAAACT ACTTTCTTCA GAAGCCCTGC AGTCCGTTTG ATATAGAGAA GTCATCCAGC 
GTCCAGAAAC AAGCTCAGCC CAAGCTCAGC ACCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238972.1
CDS	212..1030
Translation	MNLETMRIHSKAPLPGGIQDRNEKNRPSLKSLKADNRLEKSKYKPLWGKIFYLDLPSITICEKLQKDIKELGGRVEEFLSKDISYFVSNKKEAKYAQTLGRVSPVPSPESAYTAETTSPHPSHDGSSFKSQDRVCLSRGKLLAEKAVKDHDFIPANSILSNALSWGVKILHIDDIRYYIEQKKKALSALKKSSASGKDAGKKAGPGIQKTRTGRLKKPFLKVEDVNRCYRPFYLQLPSLPCINYFLQKPCSPFDIEKSSSVQKQAQPKLSTF

Target ORF information:

RefSeq Version	XM_011240670.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus DBF4 zinc finger (Dbf4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240670.2

ORF Insert Sequence:

ATGAACCTCG AGACCATGAG GATCCACAGC AAAGCACCTC TCCCGGGTGG CATCCAAGAC 
AGAAATGAAA AGAATCGACC ATCCTTGAAA TCCCTGAAGG CTGATAATAG ACTGGAAAAA 
TCCAAATATA AGCCCCTTTG GGGAAAAATA TTTTACCTTG ACTTGCCTTC TATCACCATA 
TGTGAGAAAC TACAAAAAGA TATAAAGGAA CTCGGAGGGC GAGTTGAAGA ATTTCTCAGC 
AAAGATATCA GTTATTTTGT TTCAAATAAA AAGGAAGCAA AGTATGCACA GACATTGGGT 
CGCGTTTCTC CTGTACCAAG TCCAGAATCT GCATATACTG CAGAAACCAC CTCTCCTCAT 
CCCAGCCATG ATGGAAGTTC CTTTAAGTCT CAAGACAGAG TATGTTTGAG CCGAGGAAAA 
TTGTTAGCTG AAAAAGCTGT GAAGGATCAT GATTTTATTC CTGCTAATAG TATATTATCA 
AATGCCTTAT CATGGGGAGT AAAGATTCTT CATATTGATG ACATTCGATA CTACATTGAA 
CAGAAGAAGA AAGCACTGAG TGCACTCAAG AAATCCAGTG CTTCTGGGAA GGATGCGGGG 
AAAAAAGCTG GTCCTGGAAT ACAAAAGACA AGAACAGGGA GACTCAAGAA GCCTTTTTTA 
AAGGTTGAAG ATGTGAACCG GTGCTACAGG CCATTTTACC TTCAGCTCCC CAGTCTGCCG 
TGTATAAACT ACTTTCTTCA GAAGCCCTGC AGTCCGTTTG ATATAGAGAA GTCATCCAGC 
GTCCAGAAAC AAGCTCAGCC CAAGCTCAGC ACCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
PloS one12(7)e0180682(2017)
Sundberg JP,Dadras SS,Silva KA,Kennedy VE,Garland G,Murray SA,Sundberg BA,Schofield PN,Pratt CH

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst.
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Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells.
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Kneissl M,P?tter V,Szalay AA,Grummt F

Expression of genes involved in mammalian meiosis during the transition from egg to embryo.
Molecular reproduction and development59(2)144-58(2001 Jun)
Hwang SY,Oh B,Knowles BB,Solter D,Lee JS