Pask cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pask
Entrez Gene ID	269224
Full Name	PAS domain containing serine/threonine kinase
Synonyms	mKIAA0135
General protein information	Preferred Names PAS domain containing serine/threonine kinase Names PAS domain-containing serine/threonine-protein kinase PAS-kinase paskin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 D

mRNA and Protein(s)

mRNA	Protein	Name
XM_006529665.2	XP_006529728.1	PAS domain-containing serine/threonine-protein kinase isoform X1
XM_006529665.4	XP_006529728.1	PAS domain-containing serine/threonine-protein kinase isoform X1
XM_006529666.2	XP_006529729.1	PAS domain-containing serine/threonine-protein kinase isoform X1
XM_006529666.3	XP_006529729.1	PAS domain-containing serine/threonine-protein kinase isoform X1
NM_080850.2	NP_543126.2	PAS domain-containing serine/threonine-protein kinase
XM_006529665.3	XP_006529728.1	PAS domain-containing serine/threonine-protein kinase isoform X1
XM_006529667.2	XP_006529730.1	PAS domain-containing serine/threonine-protein kinase isoform X1
XM_017321037.1	XP_017176526.1	PAS domain-containing serine/threonine-protein kinase isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PASK	NP_001239048.1
Canis lupus familiaris (dog)	PASK	XP_005636031.1
Mus musculus (house mouse)	Pask	NP_543126.2
Pan troglodytes (chimpanzee)	PASK	XP_003950058.1
Rattus norvegicus (Norway rat)	Pask	NP_001009362.1

Gene Ontology
Bibliography

Related articles in PubMed

Cell type-specific deletion in mice reveals roles for PAS kinase in insulin and glucagon production.
Semplici F, Mondragon A, Macintyre B, Madeyski-Bengston K, Persson-Kry A, Barr S, Ramne A, Marley A, McGinty J, French P, Soedling H, Yokosuka R, Gaitan J, Lang J, Migrenne-Li S, Philippe E, Herrera PL, Magnan C, da Silva Xavier G, Rutter GA
Diabetologia59(9)1938-47(2016 Sep)

Pask integrates hormonal signaling with histone modification via Wdr5 phosphorylation to drive myogenesis.
Kikani CK, Wu X, Paul L, Sabic H, Shen Z, Shakya A, Keefe A, Villanueva C, Kardon G, Graves B, Tantin D, Rutter J
eLife5(2016 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The findings demonstrate cell-autonomous roles for PASK in the control of pancreatic endocrine hormone secretion. Differences between the glycaemic phenotype of global vs cell type-specific null mice suggest important roles for tissue interactions in the control of glycaemia by PASK.
Title: Cell type-specific deletion in mice reveals roles for PAS kinase in insulin and glucagon production.

the PAS kinase function could be critical for preserving the nutrient effect on AMP-activated protein kinase and mammalian target of rapamycin/S6K1 pathways and maintain the regulatory role of exendin-4 in food intake
Title: PAS kinase is a nutrient and energy sensor in hypothalamic areas required for the normal function of AMPK and mTOR/S6K1.

PASK is a key kinase in GLP-1 actions and exerts a coordinated response with the other metabolic sensors.
Title: PAS kinase as a nutrient sensor in neuroblastoma and hypothalamic cells required for the normal expression and activity of other cellular nutrient and energy sensors.

PASK phosphorylates and inactivates GSK3beta, thereby preventing PDX-1 serine phosphorylation and alleviating GSK3beta-mediated PDX-1 protein degradation in pancreatic beta-cells.
Title: Per-Arnt-Sim kinase regulates pancreatic duodenal homeobox-1 protein stability via phosphorylation of glycogen synthase kinase 3? in pancreatic ?-cells.

These data strongly suggest that PASK is required for normal nutrient partitioning and energy homeostasis in the mouse and that this activity is primarily carried out in a cell-autonomous fashion.[Review]
Title: PAS kinase: integrating nutrient sensing with nutrient partitioning.

The following Pask gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pask cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu21506	XM_006529665.4 Latest version!	Mus musculus PAS domain containing serine/threonine kinase (Pask), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu21506	XM_006529666.3 Latest version!	Mus musculus PAS domain containing serine/threonine kinase (Pask), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu21506	NM_080850.2 Latest version!	Mus musculus PAS domain containing serine/threonine kinase (Pask), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu21506	XM_017321037.1 Latest version!	Mus musculus PAS domain containing serine/threonine kinase (Pask), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu21506	XM_006529667.2 Latest version!	Mus musculus PAS domain containing serine/threonine kinase (Pask), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OMu21506	XM_006529665.2	Mus musculus PAS domain containing serine/threonine kinase (Pask), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu21506	XM_006529666.2	Mus musculus PAS domain containing serine/threonine kinase (Pask), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu21506	XM_006529665.3	Mus musculus PAS domain containing serine/threonine kinase (Pask), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pask ( XM_006529665.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529728.1 Mus musculus PAS domain containing serine/threonine kinase (Pask), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu21506
Clone ID Related Accession (Same CDS sequence)	XM_006529665.2 , XM_006529665.4 , XM_006529666.2 , XM_006529666.3 , NM_080850.2 , XM_006529665.3 , XM_006529667.2 , XM_017321037.1
Accession Version	XM_006529665.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	PAS domain-containing serine/threonine-protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529665.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GAGGTCCACC AGTTTTTGCT GAGGACTGGA AATGTTTGTC TGAGAGTCCT 
CCTGTCCAAG AAGGCCCAGC GGCCCAGGCT ACCTTTGAGC CCAGCAAGCC ACTCTCCATA 
GCCCACAAAC ACCTGAGCAG GAAGAATGGG CTTTCTAGAC TCTGCCAGAG CCGAATGGCG 
CTCTCTGAGG ACAGGTGGAG CTCTTACTGT CTTTCATCAT TGGCTGCCCA AAACATCTGC 
ACAAGCAAAC TGCACTGCGC TGCTGCACCT GAGTATGCTG ACCCTACAGC TGGGCCACTG 
GGCAGTACTT CCTGCTGCTC CTTGCTGCGT GGCCTGGCTT CAGGATGCTC TGGGTCCCTG 
CTTTCGACCC CTGTGTGCAA CCCCAACAAG GCTGTCTTTA CCGTGGATGC CAAGACCACA 
GAGATTCTGG TTGCCAACGA CAAAGCCTGC AGCCTCCTAG GGTATAGCAG TCATGACCTG 
ATTGGCCAAA AGCTCGCCCA ATTCTTCCTC AAATCTGATT CCGAGGTGGT GGAAGCCCTC 
AGCGAGGAAC ATGTGGAGGC TGATGGCCAC GCTGCAGTGG TGTTCGGCAC AGTGGTGGAC 
ATCGTCAGCC GAATTGGGGA GAAGATTCCA GTCTCTGTGT GGATAAAGAG ATTGCAGCAA 
GATCGTGGCT TGTGCTGTGT AGTGGTTCTG GAGCCTGTGG AGAGAGTGTC AGCCTGGGTC 
GCCTTCCAGA GTGATGGAAC TATCACATCA TGCGACAGTC TCTTTGCTCA TCTCCATGGG 
TTCACATCTC CAAAGGATGT AGTTGGGCAG TGTGTCATAG ACTTGATTCC ATCCATGCAG 
CTCCCTCCTC CTGGCCAACA TATCCCAAAG AGTCTCAAGA TTCAAAGGTC TGTTGGGAGA 
GCCAGAGACG GCACCACCTT CCCTCTCAGT TTGAAGCTGA AATCCAAGCC CAGTGGAAGG 
GCTGTAGCTG ACAGTGAGGC GGCCTCTGAA CCAGGTTACC AGGCTTCTGT GTGGGTGTTC 
TGCACCATCA GTGGCCTCAT CACTCTGCTG CCAGATGGCA CAATTTATGG AGTCAACCAC 
AGCTTTGCAC TAATGCTGTT TGGTTATGGA AAGACAGAGC TCCTGGGCAA GAATATCACT 
TTCCTGATTC CTGGTTTCTA CCACTACATG GACCTCACAT ATGATAGTTC GGTACAGCTG 
CCAGATCTGG TCAATTGCCT CGACATTGGC AGAAAGAGTG GGCCTGGGGA GATGAACTCT 
GATGCCCAAC ACAACTGGGA ACTGGCCAGT GGGGCCCAGG GTCCAAGAAT TGACGTGGTG 
TTGGCACGGG ACCACATGCC ATCACAAGAT GAGACCCTGA AGCTGGTGGG AGGACAAGTC 
TCCTCTAGGA CTCAGACACG ATTGGAAACT GGATACAAGA TACTTCCTTC TTCTGCATGT 
CAGCCCTCAC TGGGGGTGGA CAGCAACCCA GAGGATGGTG AGCAGTCATT GCTCACAGAC 
CAGCAAAGCA TTCCAAAGAG AAACCTGCCA GCCCATGGTG GTCAGAACCA GCTAGACACC 
TCAGAAATAT CCCTACCAGT CCTTAAAGAA CATTTATTGT CTGAAATTCA GAAAAATATC 
TCAGAAGAAA GCCCACTAAC CCACAGGAAA TGGTTATCCA AGGTGCAACA AAACCCCACA 
AAAGGAAGCC TGCCCATCCA CGAGGAGCAG CTATTGTTTG CAGGCCAGCA TATCCATGTC 
TTGGGAAAGG AGGATCCTTC AGCAGCAGAG AGCTACAGGG AGAGTCTTTT AGAAGAAAGC 
AAGTCCAAAC CAGTGGATGC AAAACTGTTT GCTTCATGTG AAGATTCTGA GCCTCTAGTT 
TCAGTTAAGG ACAGGGGCAG CAGTGTGGAC ACGTGTAATC TGCATCAGGA AGCTCAGTTG 
GAGCTGATGG GTGTCAGCAG TCCTAACCCC TGGGCTGATG CCACCATGCC TGAGCCCCAC 
ACCACAGGGC AGATAGCAGG CGGGAGCCTT ACATATTGCC CACAGTACAG AAGCGAGTGG 
GCCTCACAGC AGCGAGGCCA GGACTCGGCT CCCAGCCCCT CTGGAATGGC GTGTGTCTTG 
CTTGGGACAC CCACTCTGGA TGAACCATGG CCAGGAGTGA GAAATGACAG AGAAGAGCTG 
CAGACTTGCT TGATTAAGGA ACAGTTATCC AAGTCAAGCT GTGAGGGAAA TCTGGGCATC 
TCCCGTGTGG AACTCGTTCC TGAAGAACAT CCTCCTTTCA CCGCCCCCGT GTCTTTCTGT 
GATCTGGGAG GCCGAGATCT ACACGCTAGC CGCTCAGGCA GCTCCTCAGC CTGCTATGCT 
TTGGCCACCG ACCTCCCTGG TGTCCTGGAG GCAGTGGAAG CTCAGGAGGC TGATGTGAAT 
TCTTATTCCT GGAACCTCAA GGAGCTCTTT CTCAAGGACC AGACAGACAG AACTCCATCA 
CATTGTTCCT GTACCACATC TGAACTCAGC GAGGCGCCAT CTCTTTCAGT GGTGGGGTCA 
GATTTAGATG TGGGTATTCT GCATAGGCAG ACTTCAGATA TTTTAGTTGA CCGAGAGATG 
TTGCTCCTTA CTGGGACCTA TTTTGACCTT AGTGAAGGCC AACGGTTCCA GGAGATGGGA 
GCAGGACATG ATCGAGCAGA ACTGTCTAAC ATTTCTTTGG TATCCTCTGA ACACTATGAA 
ACTAGTGACA TAGAAAGCCC AGGTTGTGAC CCTCCCTTAC CAGATCCTGG TCCCAACGAT 
ATGTGCCTAT CAGCAGAGAA GCCCAGGCCG AGTGCCCAGA TCACTTCCAC TCCTGTGGCA 
CGTGGGGCCA CCAGCCTGCA GCAGGAGATC CAAGAGGGCA TCTACTCTGG AAGCTGCTAC 
CACCGGGATG GCTTACAACT GAGTATACAG TTTGAAGTCA AGCGTGTGGA GCTCCAGGGC 
TCTGCGACCC TGTTCTGCTG CTGGCTGGTG AAGGACCTCT TCCATAGCCA CCGGGACTCA 
GCCACACGAA CCCGCCTGTT CCTTGCCAGT CTGCCTAGCT CTACCCACTC CATGCCTGAG 
CTGTCTGGAT CCAGCTTTGG AGAGGTGCTC AGAGCCAAGC CTTGGTTTGA GGAATCCCCA 
ACACCTGCAG AGCTAGAGGG GCTGGCGGCC TGTGAGGGCG AGTACGACTA TAAGTATAAC 
ACCATCAGCC CACTTGGCAG TGGGGCCTTT GGCTTTGTGT GGACTGCAGT GGAGAAGGAA 
TGCAACAAAG AGGTGGTGGT GAAGTTTATT AAGAAGGAGA AGGTTTTAGA GGATTGCTGG 
ATTGAGGATC CCAAACTTGG AAGAGTTACT TTAGAGATTG CAATTCTGTC CAAGGTGGAC 
CATGCCAACA TAATCAAGGT TCTGGATATA TTCGAAAACC AAGAGTTCTT CCAGCTGGTG 
ATGGAAAAGC ACGGCTCTGG CATGGACCTC TTTGCTTTCA TTGACCACCA TCCCTGCTTG 
GATGAGCCCC TGGCGAGTTT CATCTTTCGA CAACTAGTGT CTGCTGTAGG ATACCTGCAC 
TCCCAAGGCA TCATCCATAG AGACATCAAG GATGAGAACA TTGTGATTGC TGAGGACTTC 
ACAATTAAGC TGATAGATTT TGGCTCAGCT GCCTACTTAG AGAGGGGCAA ACTATTTTAT 
ACCTTTTGTG GAACAATCGA ATACTGTGCA CCTGAGGTTC TCATTGGAAA TCCCTACAGA 
GGGCCAGAGC TGGAGATGTG GTCTCTGGGG GTCACCCTGT ACACGCTCAT CTTCGAGGAG 
AATCCCTTCT GTGAGGTGGA GGAGACCATG GAGGCAGTTA TTCATCCCCC ATTCCTGGTT 
TCCCAAGAAC TTATGAGTCT TCTGTCTGGA CTGCTGCAGC CTTGCCCTGA GCAGCGGACC 
ACTTTGGAGA AGCTGATCAG GGACCCCTGG GTGACACAGC CTGTGAACCT TGCTAGCTAT 
ACTTGGGAAG AGGTGTGTAG GACCAACCAG CCAGAAAGTG GCCTGCTGTC AGCTGCAAGT 
CTGGAGATTG GGAGTAGGAG TCCAAGTGAA ATGGCTCAGA GAGAGGGTCT CTGTGGGCCT 
CCTGCTCCCA GGGAGACTCG TGGTGACCAG CACTGCTTGC ATCTTAAGGA CCCCTCTTTG 
CCAGTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529728.1
CDS	148..4299
Translation	MEDRGPPVFAEDWKCLSESPPVQEGPAAQATFEPSKPLSIAHKHLSRKNGLSRLCQSRMALSEDRWSSYCLSSLAAQNICTSKLHCAAAPEYADPTAGPLGSTSCCSLLRGLASGCSGSLLSTPVCNPNKAVFTVDAKTTEILVANDKACSLLGYSSHDLIGQKLAQFFLKSDSEVVEALSEEHVEADGHAAVVFGTVVDIVSRIGEKIPVSVWIKRLQQDRGLCCVVVLEPVERVSAWVAFQSDGTITSCDSLFAHLHGFTSPKDVVGQCVIDLIPSMQLPPPGQHIPKSLKIQRSVGRARDGTTFPLSLKLKSKPSGRAVADSEAASEPGYQASVWVFCTISGLITLLPDGTIYGVNHSFALMLFGYGKTELLGKNITFLIPGFYHYMDLTYDSSVQLPDLVNCLDIGRKSGPGEMNSDAQHNWELASGAQGPRIDVVLARDHMPSQDETLKLVGGQVSSRTQTRLETGYKILPSSACQPSLGVDSNPEDGEQSLLTDQQSIPKRNLPAHGGQNQLDTSEISLPVLKEHLLSEIQKNISEESPLTHRKWLSKVQQNPTKGSLPIHEEQLLFAGQHIHVLGKEDPSAAESYRESLLEESKSKPVDAKLFASCEDSEPLVSVKDRGSSVDTCNLHQEAQLELMGVSSPNPWADATMPEPHTTGQIAGGSLTYCPQYRSEWASQQRGQDSAPSPSGMACVLLGTPTLDEPWPGVRNDREELQTCLIKEQLSKSSCEGNLGISRVELVPEEHPPFTAPVSFCDLGGRDLHASRSGSSSACYALATDLPGVLEAVEAQEADVNSYSWNLKELFLKDQTDRTPSHCSCTTSELSEAPSLSVVGSDLDVGILHRQTSDILVDREMLLLTGTYFDLSEGQRFQEMGAGHDRAELSNISLVSSEHYETSDIESPGCDPPLPDPGPNDMCLSAEKPRPSAQITSTPVARGATSLQQEIQEGIYSGSCYHRDGLQLSIQFEVKRVELQGSATLFCCWLVKDLFHSHRDSATRTRLFLASLPSSTHSMPELSGSSFGEVLRAKPWFEESPTPAELEGLAACEGEYDYKYNTISPLGSGAFGFVWTAVEKECNKEVVVKFIKKEKVLEDCWIEDPKLGRVTLEIAILSKVDHANIIKVLDIFENQEFFQLVMEKHGSGMDLFAFIDHHPCLDEPLASFIFRQLVSAVGYLHSQGIIHRDIKDENIVIAEDFTIKLIDFGSAAYLERGKLFYTFCGTIEYCAPEVLIGNPYRGPELEMWSLGVTLYTLIFEENPFCEVEETMEAVIHPPFLVSQELMSLLSGLLQPCPEQRTTLEKLIRDPWVTQPVNLASYTWEEVCRTNQPESGLLSAASLEIGSRSPSEMAQREGLCGPPAPRETRGDQHCLHLKDPSLPVS

Target ORF information:

RefSeq Version	XM_006529665.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus PAS domain containing serine/threonine kinase (Pask), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529665.4

ORF Insert Sequence:

ATGGAGGACA GAGGTCCACC AGTTTTTGCT GAGGACTGGA AATGTTTGTC TGAGAGTCCT 
CCTGTCCAAG AAGGCCCAGC GGCCCAGGCT ACCTTTGAGC CCAGCAAGCC ACTCTCCATA 
GCCCACAAAC ACCTGAGCAG GAAGAATGGG CTTTCTAGAC TCTGCCAGAG CCGAATGGCG 
CTCTCTGAGG ACAGGTGGAG CTCTTACTGT CTTTCATCAT TGGCTGCCCA AAACATCTGC 
ACAAGCAAAC TGCACTGCGC TGCTGCACCT GAGTATGCTG ACCCTACAGC TGGGCCACTG 
GGCAGTACTT CCTGCTGCTC CTTGCTGCGT GGCCTGGCTT CAGGATGCTC TGGGTCCCTG 
CTTTCGACCC CTGTGTGCAA CCCCAACAAG GCTGTCTTTA CCGTGGATGC CAAGACCACA 
GAGATTCTGG TTGCCAACGA CAAAGCCTGC AGCCTCCTAG GGTATAGCAG TCATGACCTG 
ATTGGCCAAA AGCTCGCCCA ATTCTTCCTC AAATCTGATT CCGAGGTGGT GGAAGCCCTC 
AGCGAGGAAC ATGTGGAGGC TGATGGCCAC GCTGCAGTGG TGTTCGGCAC AGTGGTGGAC 
ATCGTCAGCC GAATTGGGGA GAAGATTCCA GTCTCTGTGT GGATAAAGAG ATTGCAGCAA 
GATCGTGGCT TGTGCTGTGT AGTGGTTCTG GAGCCTGTGG AGAGAGTGTC AGCCTGGGTC 
GCCTTCCAGA GTGATGGAAC TATCACATCA TGCGACAGTC TCTTTGCTCA TCTCCATGGG 
TTCACATCTC CAAAGGATGT AGTTGGGCAG TGTGTCATAG ACTTGATTCC ATCCATGCAG 
CTCCCTCCTC CTGGCCAACA TATCCCAAAG AGTCTCAAGA TTCAAAGGTC TGTTGGGAGA 
GCCAGAGACG GCACCACCTT CCCTCTCAGT TTGAAGCTGA AATCCAAGCC CAGTGGAAGG 
GCTGTAGCTG ACAGTGAGGC GGCCTCTGAA CCAGGTTACC AGGCTTCTGT GTGGGTGTTC 
TGCACCATCA GTGGCCTCAT CACTCTGCTG CCAGATGGCA CAATTTATGG AGTCAACCAC 
AGCTTTGCAC TAATGCTGTT TGGTTATGGA AAGACAGAGC TCCTGGGCAA GAATATCACT 
TTCCTGATTC CTGGTTTCTA CCACTACATG GACCTCACAT ATGATAGTTC GGTACAGCTG 
CCAGATCTGG TCAATTGCCT CGACATTGGC AGAAAGAGTG GGCCTGGGGA GATGAACTCT 
GATGCCCAAC ACAACTGGGA ACTGGCCAGT GGGGCCCAGG GTCCAAGAAT TGACGTGGTG 
TTGGCACGGG ACCACATGCC ATCACAAGAT GAGACCCTGA AGCTGGTGGG AGGACAAGTC 
TCCTCTAGGA CTCAGACACG ATTGGAAACT GGATACAAGA TACTTCCTTC TTCTGCATGT 
CAGCCCTCAC TGGGGGTGGA CAGCAACCCA GAGGATGGTG AGCAGTCATT GCTCACAGAC 
CAGCAAAGCA TTCCAAAGAG AAACCTGCCA GCCCATGGTG GTCAGAACCA GCTAGACACC 
TCAGAAATAT CCCTACCAGT CCTTAAAGAA CATTTATTGT CTGAAATTCA GAAAAATATC 
TCAGAAGAAA GCCCACTAAC CCACAGGAAA TGGTTATCCA AGGTGCAACA AAACCCCACA 
AAAGGAAGCC TGCCCATCCA CGAGGAGCAG CTATTGTTTG CAGGCCAGCA TATCCATGTC 
TTGGGAAAGG AGGATCCTTC AGCAGCAGAG AGCTACAGGG AGAGTCTTTT AGAAGAAAGC 
AAGTCCAAAC CAGTGGATGC AAAACTGTTT GCTTCATGTG AAGATTCTGA GCCTCTAGTT 
TCAGTTAAGG ACAGGGGCAG CAGTGTGGAC ACGTGTAATC TGCATCAGGA AGCTCAGTTG 
GAGCTGATGG GTGTCAGCAG TCCTAACCCC TGGGCTGATG CCACCATGCC TGAGCCCCAC 
ACCACAGGGC AGATAGCAGG CGGGAGCCTT ACATATTGCC CACAGTACAG AAGCGAGTGG 
GCCTCACAGC AGCGAGGCCA GGACTCGGCT CCCAGCCCCT CTGGAATGGC GTGTGTCTTG 
CTTGGGACAC CCACTCTGGA TGAACCATGG CCAGGAGTGA GAAATGACAG AGAAGAGCTG 
CAGACTTGCT TGATTAAGGA ACAGTTATCC AAGTCAAGCT GTGAGGGAAA TCTGGGCATC 
TCCCGTGTGG AACTCGTTCC TGAAGAACAT CCTCCTTTCA CCGCCCCCGT GTCTTTCTGT 
GATCTGGGAG GCCGAGATCT ACACGCTAGC CGCTCAGGCA GCTCCTCAGC CTGCTATGCT 
TTGGCCACCG ACCTCCCTGG TGTCCTGGAG GCAGTGGAAG CTCAGGAGGC TGATGTGAAT 
TCTTATTCCT GGAACCTCAA GGAGCTCTTT CTCAAGGACC AGACAGACAG AACTCCATCA 
CATTGTTCCT GTACCACATC TGAACTCAGC GAGGCGCCAT CTCTTTCAGT GGTGGGGTCA 
GATTTAGATG TGGGTATTCT GCATAGGCAG ACTTCAGATA TTTTAGTTGA CCGAGAGATG 
TTGCTCCTTA CTGGGACCTA TTTTGACCTT AGTGAAGGCC AACGGTTCCA GGAGATGGGA 
GCAGGACATG ATCGAGCAGA ACTGTCTAAC ATTTCTTTGG TATCCTCTGA ACACTATGAA 
ACTAGTGACA TAGAAAGCCC AGGTTGTGAC CCTCCCTTAC CAGATCCTGG TCCCAACGAT 
ATGTGCCTAT CAGCAGAGAA GCCCAGGCCG AGTGCCCAGA TCACTTCCAC TCCTGTGGCA 
CGTGGGGCCA CCAGCCTGCA GCAGGAGATC CAAGAGGGCA TCTACTCTGG AAGCTGCTAC 
CACCGGGATG GCTTACAACT GAGTATACAG TTTGAAGTCA AGCGTGTGGA GCTCCAGGGC 
TCTGCGACCC TGTTCTGCTG CTGGCTGGTG AAGGACCTCT TCCATAGCCA CCGGGACTCA 
GCCACACGAA CCCGCCTGTT CCTTGCCAGT CTGCCTAGCT CTACCCACTC CATGCCTGAG 
CTGTCTGGAT CCAGCTTTGG AGAGGTGCTC AGAGCCAAGC CTTGGTTTGA GGAATCCCCA 
ACACCTGCAG AGCTAGAGGG GCTGGCGGCC TGTGAGGGCG AGTACGACTA TAAGTATAAC 
ACCATCAGCC CACTTGGCAG TGGGGCCTTT GGCTTTGTGT GGACTGCAGT GGAGAAGGAA 
TGCAACAAAG AGGTGGTGGT GAAGTTTATT AAGAAGGAGA AGGTTTTAGA GGATTGCTGG 
ATTGAGGATC CCAAACTTGG AAGAGTTACT TTAGAGATTG CAATTCTGTC CAAGGTGGAC 
CATGCCAACA TAATCAAGGT TCTGGATATA TTCGAAAACC AAGAGTTCTT CCAGCTGGTG 
ATGGAAAAGC ACGGCTCTGG CATGGACCTC TTTGCTTTCA TTGACCACCA TCCCTGCTTG 
GATGAGCCCC TGGCGAGTTT CATCTTTCGA CAACTAGTGT CTGCTGTAGG ATACCTGCAC 
TCCCAAGGCA TCATCCATAG AGACATCAAG GATGAGAACA TTGTGATTGC TGAGGACTTC 
ACAATTAAGC TGATAGATTT TGGCTCAGCT GCCTACTTAG AGAGGGGCAA ACTATTTTAT 
ACCTTTTGTG GAACAATCGA ATACTGTGCA CCTGAGGTTC TCATTGGAAA TCCCTACAGA 
GGGCCAGAGC TGGAGATGTG GTCTCTGGGG GTCACCCTGT ACACGCTCAT CTTCGAGGAG 
AATCCCTTCT GTGAGGTGGA GGAGACCATG GAGGCAGTTA TTCATCCCCC ATTCCTGGTT 
TCCCAAGAAC TTATGAGTCT TCTGTCTGGA CTGCTGCAGC CTTGCCCTGA GCAGCGGACC 
ACTTTGGAGA AGCTGATCAG GGACCCCTGG GTGACACAGC CTGTGAACCT TGCTAGCTAT 
ACTTGGGAAG AGGTGTGTAG GACCAACCAG CCAGAAAGTG GCCTGCTGTC AGCTGCAAGT 
CTGGAGATTG GGAGTAGGAG TCCAAGTGAA ATGGCTCAGA GAGAGGGTCT CTGTGGGCCT 
CCTGCTCCCA GGGAGACTCG TGGTGACCAG CACTGCTTGC ATCTTAAGGA CCCCTCTTTG 
CCAGTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pask ( XM_006529666.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529729.1 Mus musculus PAS domain containing serine/threonine kinase (Pask), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu21506
Clone ID Related Accession (Same CDS sequence)	XM_006529665.2 , XM_006529665.4 , XM_006529666.2 , XM_006529666.3 , NM_080850.2 , XM_006529665.3 , XM_006529667.2 , XM_017321037.1
Accession Version	XM_006529666.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	PAS domain-containing serine/threonine-protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529666.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GAGGTCCACC AGTTTTTGCT GAGGACTGGA AATGTTTGTC TGAGAGTCCT 
CCTGTCCAAG AAGGCCCAGC GGCCCAGGCT ACCTTTGAGC CCAGCAAGCC ACTCTCCATA 
GCCCACAAAC ACCTGAGCAG GAAGAATGGG CTTTCTAGAC TCTGCCAGAG CCGAATGGCG 
CTCTCTGAGG ACAGGTGGAG CTCTTACTGT CTTTCATCAT TGGCTGCCCA AAACATCTGC 
ACAAGCAAAC TGCACTGCGC TGCTGCACCT GAGTATGCTG ACCCTACAGC TGGGCCACTG 
GGCAGTACTT CCTGCTGCTC CTTGCTGCGT GGCCTGGCTT CAGGATGCTC TGGGTCCCTG 
CTTTCGACCC CTGTGTGCAA CCCCAACAAG GCTGTCTTTA CCGTGGATGC CAAGACCACA 
GAGATTCTGG TTGCCAACGA CAAAGCCTGC AGCCTCCTAG GGTATAGCAG TCATGACCTG 
ATTGGCCAAA AGCTCGCCCA ATTCTTCCTC AAATCTGATT CCGAGGTGGT GGAAGCCCTC 
AGCGAGGAAC ATGTGGAGGC TGATGGCCAC GCTGCAGTGG TGTTCGGCAC AGTGGTGGAC 
ATCGTCAGCC GAATTGGGGA GAAGATTCCA GTCTCTGTGT GGATAAAGAG ATTGCAGCAA 
GATCGTGGCT TGTGCTGTGT AGTGGTTCTG GAGCCTGTGG AGAGAGTGTC AGCCTGGGTC 
GCCTTCCAGA GTGATGGAAC TATCACATCA TGCGACAGTC TCTTTGCTCA TCTCCATGGG 
TTCACATCTC CAAAGGATGT AGTTGGGCAG TGTGTCATAG ACTTGATTCC ATCCATGCAG 
CTCCCTCCTC CTGGCCAACA TATCCCAAAG AGTCTCAAGA TTCAAAGGTC TGTTGGGAGA 
GCCAGAGACG GCACCACCTT CCCTCTCAGT TTGAAGCTGA AATCCAAGCC CAGTGGAAGG 
GCTGTAGCTG ACAGTGAGGC GGCCTCTGAA CCAGGTTACC AGGCTTCTGT GTGGGTGTTC 
TGCACCATCA GTGGCCTCAT CACTCTGCTG CCAGATGGCA CAATTTATGG AGTCAACCAC 
AGCTTTGCAC TAATGCTGTT TGGTTATGGA AAGACAGAGC TCCTGGGCAA GAATATCACT 
TTCCTGATTC CTGGTTTCTA CCACTACATG GACCTCACAT ATGATAGTTC GGTACAGCTG 
CCAGATCTGG TCAATTGCCT CGACATTGGC AGAAAGAGTG GGCCTGGGGA GATGAACTCT 
GATGCCCAAC ACAACTGGGA ACTGGCCAGT GGGGCCCAGG GTCCAAGAAT TGACGTGGTG 
TTGGCACGGG ACCACATGCC ATCACAAGAT GAGACCCTGA AGCTGGTGGG AGGACAAGTC 
TCCTCTAGGA CTCAGACACG ATTGGAAACT GGATACAAGA TACTTCCTTC TTCTGCATGT 
CAGCCCTCAC TGGGGGTGGA CAGCAACCCA GAGGATGGTG AGCAGTCATT GCTCACAGAC 
CAGCAAAGCA TTCCAAAGAG AAACCTGCCA GCCCATGGTG GTCAGAACCA GCTAGACACC 
TCAGAAATAT CCCTACCAGT CCTTAAAGAA CATTTATTGT CTGAAATTCA GAAAAATATC 
TCAGAAGAAA GCCCACTAAC CCACAGGAAA TGGTTATCCA AGGTGCAACA AAACCCCACA 
AAAGGAAGCC TGCCCATCCA CGAGGAGCAG CTATTGTTTG CAGGCCAGCA TATCCATGTC 
TTGGGAAAGG AGGATCCTTC AGCAGCAGAG AGCTACAGGG AGAGTCTTTT AGAAGAAAGC 
AAGTCCAAAC CAGTGGATGC AAAACTGTTT GCTTCATGTG AAGATTCTGA GCCTCTAGTT 
TCAGTTAAGG ACAGGGGCAG CAGTGTGGAC ACGTGTAATC TGCATCAGGA AGCTCAGTTG 
GAGCTGATGG GTGTCAGCAG TCCTAACCCC TGGGCTGATG CCACCATGCC TGAGCCCCAC 
ACCACAGGGC AGATAGCAGG CGGGAGCCTT ACATATTGCC CACAGTACAG AAGCGAGTGG 
GCCTCACAGC AGCGAGGCCA GGACTCGGCT CCCAGCCCCT CTGGAATGGC GTGTGTCTTG 
CTTGGGACAC CCACTCTGGA TGAACCATGG CCAGGAGTGA GAAATGACAG AGAAGAGCTG 
CAGACTTGCT TGATTAAGGA ACAGTTATCC AAGTCAAGCT GTGAGGGAAA TCTGGGCATC 
TCCCGTGTGG AACTCGTTCC TGAAGAACAT CCTCCTTTCA CCGCCCCCGT GTCTTTCTGT 
GATCTGGGAG GCCGAGATCT ACACGCTAGC CGCTCAGGCA GCTCCTCAGC CTGCTATGCT 
TTGGCCACCG ACCTCCCTGG TGTCCTGGAG GCAGTGGAAG CTCAGGAGGC TGATGTGAAT 
TCTTATTCCT GGAACCTCAA GGAGCTCTTT CTCAAGGACC AGACAGACAG AACTCCATCA 
CATTGTTCCT GTACCACATC TGAACTCAGC GAGGCGCCAT CTCTTTCAGT GGTGGGGTCA 
GATTTAGATG TGGGTATTCT GCATAGGCAG ACTTCAGATA TTTTAGTTGA CCGAGAGATG 
TTGCTCCTTA CTGGGACCTA TTTTGACCTT AGTGAAGGCC AACGGTTCCA GGAGATGGGA 
GCAGGACATG ATCGAGCAGA ACTGTCTAAC ATTTCTTTGG TATCCTCTGA ACACTATGAA 
ACTAGTGACA TAGAAAGCCC AGGTTGTGAC CCTCCCTTAC CAGATCCTGG TCCCAACGAT 
ATGTGCCTAT CAGCAGAGAA GCCCAGGCCG AGTGCCCAGA TCACTTCCAC TCCTGTGGCA 
CGTGGGGCCA CCAGCCTGCA GCAGGAGATC CAAGAGGGCA TCTACTCTGG AAGCTGCTAC 
CACCGGGATG GCTTACAACT GAGTATACAG TTTGAAGTCA AGCGTGTGGA GCTCCAGGGC 
TCTGCGACCC TGTTCTGCTG CTGGCTGGTG AAGGACCTCT TCCATAGCCA CCGGGACTCA 
GCCACACGAA CCCGCCTGTT CCTTGCCAGT CTGCCTAGCT CTACCCACTC CATGCCTGAG 
CTGTCTGGAT CCAGCTTTGG AGAGGTGCTC AGAGCCAAGC CTTGGTTTGA GGAATCCCCA 
ACACCTGCAG AGCTAGAGGG GCTGGCGGCC TGTGAGGGCG AGTACGACTA TAAGTATAAC 
ACCATCAGCC CACTTGGCAG TGGGGCCTTT GGCTTTGTGT GGACTGCAGT GGAGAAGGAA 
TGCAACAAAG AGGTGGTGGT GAAGTTTATT AAGAAGGAGA AGGTTTTAGA GGATTGCTGG 
ATTGAGGATC CCAAACTTGG AAGAGTTACT TTAGAGATTG CAATTCTGTC CAAGGTGGAC 
CATGCCAACA TAATCAAGGT TCTGGATATA TTCGAAAACC AAGAGTTCTT CCAGCTGGTG 
ATGGAAAAGC ACGGCTCTGG CATGGACCTC TTTGCTTTCA TTGACCACCA TCCCTGCTTG 
GATGAGCCCC TGGCGAGTTT CATCTTTCGA CAACTAGTGT CTGCTGTAGG ATACCTGCAC 
TCCCAAGGCA TCATCCATAG AGACATCAAG GATGAGAACA TTGTGATTGC TGAGGACTTC 
ACAATTAAGC TGATAGATTT TGGCTCAGCT GCCTACTTAG AGAGGGGCAA ACTATTTTAT 
ACCTTTTGTG GAACAATCGA ATACTGTGCA CCTGAGGTTC TCATTGGAAA TCCCTACAGA 
GGGCCAGAGC TGGAGATGTG GTCTCTGGGG GTCACCCTGT ACACGCTCAT CTTCGAGGAG 
AATCCCTTCT GTGAGGTGGA GGAGACCATG GAGGCAGTTA TTCATCCCCC ATTCCTGGTT 
TCCCAAGAAC TTATGAGTCT TCTGTCTGGA CTGCTGCAGC CTTGCCCTGA GCAGCGGACC 
ACTTTGGAGA AGCTGATCAG GGACCCCTGG GTGACACAGC CTGTGAACCT TGCTAGCTAT 
ACTTGGGAAG AGGTGTGTAG GACCAACCAG CCAGAAAGTG GCCTGCTGTC AGCTGCAAGT 
CTGGAGATTG GGAGTAGGAG TCCAAGTGAA ATGGCTCAGA GAGAGGGTCT CTGTGGGCCT 
CCTGCTCCCA GGGAGACTCG TGGTGACCAG CACTGCTTGC ATCTTAAGGA CCCCTCTTTG 
CCAGTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529729.1
CDS	307..4458
Translation	MEDRGPPVFAEDWKCLSESPPVQEGPAAQATFEPSKPLSIAHKHLSRKNGLSRLCQSRMALSEDRWSSYCLSSLAAQNICTSKLHCAAAPEYADPTAGPLGSTSCCSLLRGLASGCSGSLLSTPVCNPNKAVFTVDAKTTEILVANDKACSLLGYSSHDLIGQKLAQFFLKSDSEVVEALSEEHVEADGHAAVVFGTVVDIVSRIGEKIPVSVWIKRLQQDRGLCCVVVLEPVERVSAWVAFQSDGTITSCDSLFAHLHGFTSPKDVVGQCVIDLIPSMQLPPPGQHIPKSLKIQRSVGRARDGTTFPLSLKLKSKPSGRAVADSEAASEPGYQASVWVFCTISGLITLLPDGTIYGVNHSFALMLFGYGKTELLGKNITFLIPGFYHYMDLTYDSSVQLPDLVNCLDIGRKSGPGEMNSDAQHNWELASGAQGPRIDVVLARDHMPSQDETLKLVGGQVSSRTQTRLETGYKILPSSACQPSLGVDSNPEDGEQSLLTDQQSIPKRNLPAHGGQNQLDTSEISLPVLKEHLLSEIQKNISEESPLTHRKWLSKVQQNPTKGSLPIHEEQLLFAGQHIHVLGKEDPSAAESYRESLLEESKSKPVDAKLFASCEDSEPLVSVKDRGSSVDTCNLHQEAQLELMGVSSPNPWADATMPEPHTTGQIAGGSLTYCPQYRSEWASQQRGQDSAPSPSGMACVLLGTPTLDEPWPGVRNDREELQTCLIKEQLSKSSCEGNLGISRVELVPEEHPPFTAPVSFCDLGGRDLHASRSGSSSACYALATDLPGVLEAVEAQEADVNSYSWNLKELFLKDQTDRTPSHCSCTTSELSEAPSLSVVGSDLDVGILHRQTSDILVDREMLLLTGTYFDLSEGQRFQEMGAGHDRAELSNISLVSSEHYETSDIESPGCDPPLPDPGPNDMCLSAEKPRPSAQITSTPVARGATSLQQEIQEGIYSGSCYHRDGLQLSIQFEVKRVELQGSATLFCCWLVKDLFHSHRDSATRTRLFLASLPSSTHSMPELSGSSFGEVLRAKPWFEESPTPAELEGLAACEGEYDYKYNTISPLGSGAFGFVWTAVEKECNKEVVVKFIKKEKVLEDCWIEDPKLGRVTLEIAILSKVDHANIIKVLDIFENQEFFQLVMEKHGSGMDLFAFIDHHPCLDEPLASFIFRQLVSAVGYLHSQGIIHRDIKDENIVIAEDFTIKLIDFGSAAYLERGKLFYTFCGTIEYCAPEVLIGNPYRGPELEMWSLGVTLYTLIFEENPFCEVEETMEAVIHPPFLVSQELMSLLSGLLQPCPEQRTTLEKLIRDPWVTQPVNLASYTWEEVCRTNQPESGLLSAASLEIGSRSPSEMAQREGLCGPPAPRETRGDQHCLHLKDPSLPVS

Target ORF information:

RefSeq Version	XM_006529666.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus PAS domain containing serine/threonine kinase (Pask), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529666.3

ORF Insert Sequence:

ATGGAGGACA GAGGTCCACC AGTTTTTGCT GAGGACTGGA AATGTTTGTC TGAGAGTCCT 
CCTGTCCAAG AAGGCCCAGC GGCCCAGGCT ACCTTTGAGC CCAGCAAGCC ACTCTCCATA 
GCCCACAAAC ACCTGAGCAG GAAGAATGGG CTTTCTAGAC TCTGCCAGAG CCGAATGGCG 
CTCTCTGAGG ACAGGTGGAG CTCTTACTGT CTTTCATCAT TGGCTGCCCA AAACATCTGC 
ACAAGCAAAC TGCACTGCGC TGCTGCACCT GAGTATGCTG ACCCTACAGC TGGGCCACTG 
GGCAGTACTT CCTGCTGCTC CTTGCTGCGT GGCCTGGCTT CAGGATGCTC TGGGTCCCTG 
CTTTCGACCC CTGTGTGCAA CCCCAACAAG GCTGTCTTTA CCGTGGATGC CAAGACCACA 
GAGATTCTGG TTGCCAACGA CAAAGCCTGC AGCCTCCTAG GGTATAGCAG TCATGACCTG 
ATTGGCCAAA AGCTCGCCCA ATTCTTCCTC AAATCTGATT CCGAGGTGGT GGAAGCCCTC 
AGCGAGGAAC ATGTGGAGGC TGATGGCCAC GCTGCAGTGG TGTTCGGCAC AGTGGTGGAC 
ATCGTCAGCC GAATTGGGGA GAAGATTCCA GTCTCTGTGT GGATAAAGAG ATTGCAGCAA 
GATCGTGGCT TGTGCTGTGT AGTGGTTCTG GAGCCTGTGG AGAGAGTGTC AGCCTGGGTC 
GCCTTCCAGA GTGATGGAAC TATCACATCA TGCGACAGTC TCTTTGCTCA TCTCCATGGG 
TTCACATCTC CAAAGGATGT AGTTGGGCAG TGTGTCATAG ACTTGATTCC ATCCATGCAG 
CTCCCTCCTC CTGGCCAACA TATCCCAAAG AGTCTCAAGA TTCAAAGGTC TGTTGGGAGA 
GCCAGAGACG GCACCACCTT CCCTCTCAGT TTGAAGCTGA AATCCAAGCC CAGTGGAAGG 
GCTGTAGCTG ACAGTGAGGC GGCCTCTGAA CCAGGTTACC AGGCTTCTGT GTGGGTGTTC 
TGCACCATCA GTGGCCTCAT CACTCTGCTG CCAGATGGCA CAATTTATGG AGTCAACCAC 
AGCTTTGCAC TAATGCTGTT TGGTTATGGA AAGACAGAGC TCCTGGGCAA GAATATCACT 
TTCCTGATTC CTGGTTTCTA CCACTACATG GACCTCACAT ATGATAGTTC GGTACAGCTG 
CCAGATCTGG TCAATTGCCT CGACATTGGC AGAAAGAGTG GGCCTGGGGA GATGAACTCT 
GATGCCCAAC ACAACTGGGA ACTGGCCAGT GGGGCCCAGG GTCCAAGAAT TGACGTGGTG 
TTGGCACGGG ACCACATGCC ATCACAAGAT GAGACCCTGA AGCTGGTGGG AGGACAAGTC 
TCCTCTAGGA CTCAGACACG ATTGGAAACT GGATACAAGA TACTTCCTTC TTCTGCATGT 
CAGCCCTCAC TGGGGGTGGA CAGCAACCCA GAGGATGGTG AGCAGTCATT GCTCACAGAC 
CAGCAAAGCA TTCCAAAGAG AAACCTGCCA GCCCATGGTG GTCAGAACCA GCTAGACACC 
TCAGAAATAT CCCTACCAGT CCTTAAAGAA CATTTATTGT CTGAAATTCA GAAAAATATC 
TCAGAAGAAA GCCCACTAAC CCACAGGAAA TGGTTATCCA AGGTGCAACA AAACCCCACA 
AAAGGAAGCC TGCCCATCCA CGAGGAGCAG CTATTGTTTG CAGGCCAGCA TATCCATGTC 
TTGGGAAAGG AGGATCCTTC AGCAGCAGAG AGCTACAGGG AGAGTCTTTT AGAAGAAAGC 
AAGTCCAAAC CAGTGGATGC AAAACTGTTT GCTTCATGTG AAGATTCTGA GCCTCTAGTT 
TCAGTTAAGG ACAGGGGCAG CAGTGTGGAC ACGTGTAATC TGCATCAGGA AGCTCAGTTG 
GAGCTGATGG GTGTCAGCAG TCCTAACCCC TGGGCTGATG CCACCATGCC TGAGCCCCAC 
ACCACAGGGC AGATAGCAGG CGGGAGCCTT ACATATTGCC CACAGTACAG AAGCGAGTGG 
GCCTCACAGC AGCGAGGCCA GGACTCGGCT CCCAGCCCCT CTGGAATGGC GTGTGTCTTG 
CTTGGGACAC CCACTCTGGA TGAACCATGG CCAGGAGTGA GAAATGACAG AGAAGAGCTG 
CAGACTTGCT TGATTAAGGA ACAGTTATCC AAGTCAAGCT GTGAGGGAAA TCTGGGCATC 
TCCCGTGTGG AACTCGTTCC TGAAGAACAT CCTCCTTTCA CCGCCCCCGT GTCTTTCTGT 
GATCTGGGAG GCCGAGATCT ACACGCTAGC CGCTCAGGCA GCTCCTCAGC CTGCTATGCT 
TTGGCCACCG ACCTCCCTGG TGTCCTGGAG GCAGTGGAAG CTCAGGAGGC TGATGTGAAT 
TCTTATTCCT GGAACCTCAA GGAGCTCTTT CTCAAGGACC AGACAGACAG AACTCCATCA 
CATTGTTCCT GTACCACATC TGAACTCAGC GAGGCGCCAT CTCTTTCAGT GGTGGGGTCA 
GATTTAGATG TGGGTATTCT GCATAGGCAG ACTTCAGATA TTTTAGTTGA CCGAGAGATG 
TTGCTCCTTA CTGGGACCTA TTTTGACCTT AGTGAAGGCC AACGGTTCCA GGAGATGGGA 
GCAGGACATG ATCGAGCAGA ACTGTCTAAC ATTTCTTTGG TATCCTCTGA ACACTATGAA 
ACTAGTGACA TAGAAAGCCC AGGTTGTGAC CCTCCCTTAC CAGATCCTGG TCCCAACGAT 
ATGTGCCTAT CAGCAGAGAA GCCCAGGCCG AGTGCCCAGA TCACTTCCAC TCCTGTGGCA 
CGTGGGGCCA CCAGCCTGCA GCAGGAGATC CAAGAGGGCA TCTACTCTGG AAGCTGCTAC 
CACCGGGATG GCTTACAACT GAGTATACAG TTTGAAGTCA AGCGTGTGGA GCTCCAGGGC 
TCTGCGACCC TGTTCTGCTG CTGGCTGGTG AAGGACCTCT TCCATAGCCA CCGGGACTCA 
GCCACACGAA CCCGCCTGTT CCTTGCCAGT CTGCCTAGCT CTACCCACTC CATGCCTGAG 
CTGTCTGGAT CCAGCTTTGG AGAGGTGCTC AGAGCCAAGC CTTGGTTTGA GGAATCCCCA 
ACACCTGCAG AGCTAGAGGG GCTGGCGGCC TGTGAGGGCG AGTACGACTA TAAGTATAAC 
ACCATCAGCC CACTTGGCAG TGGGGCCTTT GGCTTTGTGT GGACTGCAGT GGAGAAGGAA 
TGCAACAAAG AGGTGGTGGT GAAGTTTATT AAGAAGGAGA AGGTTTTAGA GGATTGCTGG 
ATTGAGGATC CCAAACTTGG AAGAGTTACT TTAGAGATTG CAATTCTGTC CAAGGTGGAC 
CATGCCAACA TAATCAAGGT TCTGGATATA TTCGAAAACC AAGAGTTCTT CCAGCTGGTG 
ATGGAAAAGC ACGGCTCTGG CATGGACCTC TTTGCTTTCA TTGACCACCA TCCCTGCTTG 
GATGAGCCCC TGGCGAGTTT CATCTTTCGA CAACTAGTGT CTGCTGTAGG ATACCTGCAC 
TCCCAAGGCA TCATCCATAG AGACATCAAG GATGAGAACA TTGTGATTGC TGAGGACTTC 
ACAATTAAGC TGATAGATTT TGGCTCAGCT GCCTACTTAG AGAGGGGCAA ACTATTTTAT 
ACCTTTTGTG GAACAATCGA ATACTGTGCA CCTGAGGTTC TCATTGGAAA TCCCTACAGA 
GGGCCAGAGC TGGAGATGTG GTCTCTGGGG GTCACCCTGT ACACGCTCAT CTTCGAGGAG 
AATCCCTTCT GTGAGGTGGA GGAGACCATG GAGGCAGTTA TTCATCCCCC ATTCCTGGTT 
TCCCAAGAAC TTATGAGTCT TCTGTCTGGA CTGCTGCAGC CTTGCCCTGA GCAGCGGACC 
ACTTTGGAGA AGCTGATCAG GGACCCCTGG GTGACACAGC CTGTGAACCT TGCTAGCTAT 
ACTTGGGAAG AGGTGTGTAG GACCAACCAG CCAGAAAGTG GCCTGCTGTC AGCTGCAAGT 
CTGGAGATTG GGAGTAGGAG TCCAAGTGAA ATGGCTCAGA GAGAGGGTCT CTGTGGGCCT 
CCTGCTCCCA GGGAGACTCG TGGTGACCAG CACTGCTTGC ATCTTAAGGA CCCCTCTTTG 
CCAGTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pask ( NM_080850.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_543126.2 Mus musculus PAS domain containing serine/threonine kinase (Pask), mRNA.

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CloneID	OMu21506
Clone ID Related Accession (Same CDS sequence)	XM_006529665.2 , XM_006529665.4 , XM_006529666.2 , XM_006529666.3 , NM_080850.2 , XM_006529665.3 , XM_006529667.2 , XM_017321037.1
Accession Version	NM_080850.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	PAS domain-containing serine/threonine-protein kinase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY058767.1, AK028435.1, AK129063.1 and AK141220.1. On Jul 19, 2008 this sequence version replaced NM_080850.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK083590.1, AK083528.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGACA GAGGTCCACC AGTTTTTGCT GAGGACTGGA AATGTTTGTC TGAGAGTCCT 
CCTGTCCAAG AAGGCCCAGC GGCCCAGGCT ACCTTTGAGC CCAGCAAGCC ACTCTCCATA 
GCCCACAAAC ACCTGAGCAG GAAGAATGGG CTTTCTAGAC TCTGCCAGAG CCGAATGGCG 
CTCTCTGAGG ACAGGTGGAG CTCTTACTGT CTTTCATCAT TGGCTGCCCA AAACATCTGC 
ACAAGCAAAC TGCACTGCGC TGCTGCACCT GAGTATGCTG ACCCTACAGC TGGGCCACTG 
GGCAGTACTT CCTGCTGCTC CTTGCTGCGT GGCCTGGCTT CAGGATGCTC TGGGTCCCTG 
CTTTCGACCC CTGTGTGCAA CCCCAACAAG GCTGTCTTTA CCGTGGATGC CAAGACCACA 
GAGATTCTGG TTGCCAACGA CAAAGCCTGC AGCCTCCTAG GGTATAGCAG TCATGACCTG 
ATTGGCCAAA AGCTCGCCCA ATTCTTCCTC AAATCTGATT CCGAGGTGGT GGAAGCCCTC 
AGCGAGGAAC ATGTGGAGGC TGATGGCCAC GCTGCAGTGG TGTTCGGCAC AGTGGTGGAC 
ATCGTCAGCC GAATTGGGGA GAAGATTCCA GTCTCTGTGT GGATAAAGAG ATTGCAGCAA 
GATCGTGGCT TGTGCTGTGT AGTGGTTCTG GAGCCTGTGG AGAGAGTGTC AGCCTGGGTC 
GCCTTCCAGA GTGATGGAAC TATCACATCA TGCGACAGTC TCTTTGCTCA TCTCCATGGG 
TTCACATCTC CAAAGGATGT AGTTGGGCAG TGTGTCATAG ACTTGATTCC ATCCATGCAG 
CTCCCTCCTC CTGGCCAACA TATCCCAAAG AGTCTCAAGA TTCAAAGGTC TGTTGGGAGA 
GCCAGAGACG GCACCACCTT CCCTCTCAGT TTGAAGCTGA AATCCAAGCC CAGTGGAAGG 
GCTGTAGCTG ACAGTGAGGC GGCCTCTGAA CCAGGTTACC AGGCTTCTGT GTGGGTGTTC 
TGCACCATCA GTGGCCTCAT CACTCTGCTG CCAGATGGCA CAATTTATGG AGTCAACCAC 
AGCTTTGCAC TAATGCTGTT TGGTTATGGA AAGACAGAGC TCCTGGGCAA GAATATCACT 
TTCCTGATTC CTGGTTTCTA CCACTACATG GACCTCACAT ATGATAGTTC GGTACAGCTG 
CCAGATCTGG TCAATTGCCT CGACATTGGC AGAAAGAGTG GGCCTGGGGA GATGAACTCT 
GATGCCCAAC ACAACTGGGA ACTGGCCAGT GGGGCCCAGG GTCCAAGAAT TGACGTGGTG 
TTGGCACGGG ACCACATGCC ATCACAAGAT GAGACCCTGA AGCTGGTGGG AGGACAAGTC 
TCCTCTAGGA CTCAGACACG ATTGGAAACT GGATACAAGA TACTTCCTTC TTCTGCATGT 
CAGCCCTCAC TGGGGGTGGA CAGCAACCCA GAGGATGGTG AGCAGTCATT GCTCACAGAC 
CAGCAAAGCA TTCCAAAGAG AAACCTGCCA GCCCATGGTG GTCAGAACCA GCTAGACACC 
TCAGAAATAT CCCTACCAGT CCTTAAAGAA CATTTATTGT CTGAAATTCA GAAAAATATC 
TCAGAAGAAA GCCCACTAAC CCACAGGAAA TGGTTATCCA AGGTGCAACA AAACCCCACA 
AAAGGAAGCC TGCCCATCCA CGAGGAGCAG CTATTGTTTG CAGGCCAGCA TATCCATGTC 
TTGGGAAAGG AGGATCCTTC AGCAGCAGAG AGCTACAGGG AGAGTCTTTT AGAAGAAAGC 
AAGTCCAAAC CAGTGGATGC AAAACTGTTT GCTTCATGTG AAGATTCTGA GCCTCTAGTT 
TCAGTTAAGG ACAGGGGCAG CAGTGTGGAC ACGTGTAATC TGCATCAGGA AGCTCAGTTG 
GAGCTGATGG GTGTCAGCAG TCCTAACCCC TGGGCTGATG CCACCATGCC TGAGCCCCAC 
ACCACAGGGC AGATAGCAGG CGGGAGCCTT ACATATTGCC CACAGTACAG AAGCGAGTGG 
GCCTCACAGC AGCGAGGCCA GGACTCGGCT CCCAGCCCCT CTGGAATGGC GTGTGTCTTG 
CTTGGGACAC CCACTCTGGA TGAACCATGG CCAGGAGTGA GAAATGACAG AGAAGAGCTG 
CAGACTTGCT TGATTAAGGA ACAGTTATCC AAGTCAAGCT GTGAGGGAAA TCTGGGCATC 
TCCCGTGTGG AACTCGTTCC TGAAGAACAT CCTCCTTTCA CCGCCCCCGT GTCTTTCTGT 
GATCTGGGAG GCCGAGATCT ACACGCTAGC CGCTCAGGCA GCTCCTCAGC CTGCTATGCT 
TTGGCCACCG ACCTCCCTGG TGTCCTGGAG GCAGTGGAAG CTCAGGAGGC TGATGTGAAT 
TCTTATTCCT GGAACCTCAA GGAGCTCTTT CTCAAGGACC AGACAGACAG AACTCCATCA 
CATTGTTCCT GTACCACATC TGAACTCAGC GAGGCGCCAT CTCTTTCAGT GGTGGGGTCA 
GATTTAGATG TGGGTATTCT GCATAGGCAG ACTTCAGATA TTTTAGTTGA CCGAGAGATG 
TTGCTCCTTA CTGGGACCTA TTTTGACCTT AGTGAAGGCC AACGGTTCCA GGAGATGGGA 
GCAGGACATG ATCGAGCAGA ACTGTCTAAC ATTTCTTTGG TATCCTCTGA ACACTATGAA 
ACTAGTGACA TAGAAAGCCC AGGTTGTGAC CCTCCCTTAC CAGATCCTGG TCCCAACGAT 
ATGTGCCTAT CAGCAGAGAA GCCCAGGCCG AGTGCCCAGA TCACTTCCAC TCCTGTGGCA 
CGTGGGGCCA CCAGCCTGCA GCAGGAGATC CAAGAGGGCA TCTACTCTGG AAGCTGCTAC 
CACCGGGATG GCTTACAACT GAGTATACAG TTTGAAGTCA AGCGTGTGGA GCTCCAGGGC 
TCTGCGACCC TGTTCTGCTG CTGGCTGGTG AAGGACCTCT TCCATAGCCA CCGGGACTCA 
GCCACACGAA CCCGCCTGTT CCTTGCCAGT CTGCCTAGCT CTACCCACTC CATGCCTGAG 
CTGTCTGGAT CCAGCTTTGG AGAGGTGCTC AGAGCCAAGC CTTGGTTTGA GGAATCCCCA 
ACACCTGCAG AGCTAGAGGG GCTGGCGGCC TGTGAGGGCG AGTACGACTA TAAGTATAAC 
ACCATCAGCC CACTTGGCAG TGGGGCCTTT GGCTTTGTGT GGACTGCAGT GGAGAAGGAA 
TGCAACAAAG AGGTGGTGGT GAAGTTTATT AAGAAGGAGA AGGTTTTAGA GGATTGCTGG 
ATTGAGGATC CCAAACTTGG AAGAGTTACT TTAGAGATTG CAATTCTGTC CAAGGTGGAC 
CATGCCAACA TAATCAAGGT TCTGGATATA TTCGAAAACC AAGAGTTCTT CCAGCTGGTG 
ATGGAAAAGC ACGGCTCTGG CATGGACCTC TTTGCTTTCA TTGACCACCA TCCCTGCTTG 
GATGAGCCCC TGGCGAGTTT CATCTTTCGA CAACTAGTGT CTGCTGTAGG ATACCTGCAC 
TCCCAAGGCA TCATCCATAG AGACATCAAG GATGAGAACA TTGTGATTGC TGAGGACTTC 
ACAATTAAGC TGATAGATTT TGGCTCAGCT GCCTACTTAG AGAGGGGCAA ACTATTTTAT 
ACCTTTTGTG GAACAATCGA ATACTGTGCA CCTGAGGTTC TCATTGGAAA TCCCTACAGA 
GGGCCAGAGC TGGAGATGTG GTCTCTGGGG GTCACCCTGT ACACGCTCAT CTTCGAGGAG 
AATCCCTTCT GTGAGGTGGA GGAGACCATG GAGGCAGTTA TTCATCCCCC ATTCCTGGTT 
TCCCAAGAAC TTATGAGTCT TCTGTCTGGA CTGCTGCAGC CTTGCCCTGA GCAGCGGACC 
ACTTTGGAGA AGCTGATCAG GGACCCCTGG GTGACACAGC CTGTGAACCT TGCTAGCTAT 
ACTTGGGAAG AGGTGTGTAG GACCAACCAG CCAGAAAGTG GCCTGCTGTC AGCTGCAAGT 
CTGGAGATTG GGAGTAGGAG TCCAAGTGAA ATGGCTCAGA GAGAGGGTCT CTGTGGGCCT 
CCTGCTCCCA GGGAGACTCG TGGTGACCAG CACTGCTTGC ATCTTAAGGA CCCCTCTTTG 
CCAGTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_543126.2
CDS	313..4464
Translation	MEDRGPPVFAEDWKCLSESPPVQEGPAAQATFEPSKPLSIAHKHLSRKNGLSRLCQSRMALSEDRWSSYCLSSLAAQNICTSKLHCAAAPEYADPTAGPLGSTSCCSLLRGLASGCSGSLLSTPVCNPNKAVFTVDAKTTEILVANDKACSLLGYSSHDLIGQKLAQFFLKSDSEVVEALSEEHVEADGHAAVVFGTVVDIVSRIGEKIPVSVWIKRLQQDRGLCCVVVLEPVERVSAWVAFQSDGTITSCDSLFAHLHGFTSPKDVVGQCVIDLIPSMQLPPPGQHIPKSLKIQRSVGRARDGTTFPLSLKLKSKPSGRAVADSEAASEPGYQASVWVFCTISGLITLLPDGTIYGVNHSFALMLFGYGKTELLGKNITFLIPGFYHYMDLTYDSSVQLPDLVNCLDIGRKSGPGEMNSDAQHNWELASGAQGPRIDVVLARDHMPSQDETLKLVGGQVSSRTQTRLETGYKILPSSACQPSLGVDSNPEDGEQSLLTDQQSIPKRNLPAHGGQNQLDTSEISLPVLKEHLLSEIQKNISEESPLTHRKWLSKVQQNPTKGSLPIHEEQLLFAGQHIHVLGKEDPSAAESYRESLLEESKSKPVDAKLFASCEDSEPLVSVKDRGSSVDTCNLHQEAQLELMGVSSPNPWADATMPEPHTTGQIAGGSLTYCPQYRSEWASQQRGQDSAPSPSGMACVLLGTPTLDEPWPGVRNDREELQTCLIKEQLSKSSCEGNLGISRVELVPEEHPPFTAPVSFCDLGGRDLHASRSGSSSACYALATDLPGVLEAVEAQEADVNSYSWNLKELFLKDQTDRTPSHCSCTTSELSEAPSLSVVGSDLDVGILHRQTSDILVDREMLLLTGTYFDLSEGQRFQEMGAGHDRAELSNISLVSSEHYETSDIESPGCDPPLPDPGPNDMCLSAEKPRPSAQITSTPVARGATSLQQEIQEGIYSGSCYHRDGLQLSIQFEVKRVELQGSATLFCCWLVKDLFHSHRDSATRTRLFLASLPSSTHSMPELSGSSFGEVLRAKPWFEESPTPAELEGLAACEGEYDYKYNTISPLGSGAFGFVWTAVEKECNKEVVVKFIKKEKVLEDCWIEDPKLGRVTLEIAILSKVDHANIIKVLDIFENQEFFQLVMEKHGSGMDLFAFIDHHPCLDEPLASFIFRQLVSAVGYLHSQGIIHRDIKDENIVIAEDFTIKLIDFGSAAYLERGKLFYTFCGTIEYCAPEVLIGNPYRGPELEMWSLGVTLYTLIFEENPFCEVEETMEAVIHPPFLVSQELMSLLSGLLQPCPEQRTTLEKLIRDPWVTQPVNLASYTWEEVCRTNQPESGLLSAASLEIGSRSPSEMAQREGLCGPPAPRETRGDQHCLHLKDPSLPVS

Target ORF information:

RefSeq Version	NM_080850.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus PAS domain containing serine/threonine kinase (Pask), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080850.2

ORF Insert Sequence:

ATGGAGGACA GAGGTCCACC AGTTTTTGCT GAGGACTGGA AATGTTTGTC TGAGAGTCCT 
CCTGTCCAAG AAGGCCCAGC GGCCCAGGCT ACCTTTGAGC CCAGCAAGCC ACTCTCCATA 
GCCCACAAAC ACCTGAGCAG GAAGAATGGG CTTTCTAGAC TCTGCCAGAG CCGAATGGCG 
CTCTCTGAGG ACAGGTGGAG CTCTTACTGT CTTTCATCAT TGGCTGCCCA AAACATCTGC 
ACAAGCAAAC TGCACTGCGC TGCTGCACCT GAGTATGCTG ACCCTACAGC TGGGCCACTG 
GGCAGTACTT CCTGCTGCTC CTTGCTGCGT GGCCTGGCTT CAGGATGCTC TGGGTCCCTG 
CTTTCGACCC CTGTGTGCAA CCCCAACAAG GCTGTCTTTA CCGTGGATGC CAAGACCACA 
GAGATTCTGG TTGCCAACGA CAAAGCCTGC AGCCTCCTAG GGTATAGCAG TCATGACCTG 
ATTGGCCAAA AGCTCGCCCA ATTCTTCCTC AAATCTGATT CCGAGGTGGT GGAAGCCCTC 
AGCGAGGAAC ATGTGGAGGC TGATGGCCAC GCTGCAGTGG TGTTCGGCAC AGTGGTGGAC 
ATCGTCAGCC GAATTGGGGA GAAGATTCCA GTCTCTGTGT GGATAAAGAG ATTGCAGCAA 
GATCGTGGCT TGTGCTGTGT AGTGGTTCTG GAGCCTGTGG AGAGAGTGTC AGCCTGGGTC 
GCCTTCCAGA GTGATGGAAC TATCACATCA TGCGACAGTC TCTTTGCTCA TCTCCATGGG 
TTCACATCTC CAAAGGATGT AGTTGGGCAG TGTGTCATAG ACTTGATTCC ATCCATGCAG 
CTCCCTCCTC CTGGCCAACA TATCCCAAAG AGTCTCAAGA TTCAAAGGTC TGTTGGGAGA 
GCCAGAGACG GCACCACCTT CCCTCTCAGT TTGAAGCTGA AATCCAAGCC CAGTGGAAGG 
GCTGTAGCTG ACAGTGAGGC GGCCTCTGAA CCAGGTTACC AGGCTTCTGT GTGGGTGTTC 
TGCACCATCA GTGGCCTCAT CACTCTGCTG CCAGATGGCA CAATTTATGG AGTCAACCAC 
AGCTTTGCAC TAATGCTGTT TGGTTATGGA AAGACAGAGC TCCTGGGCAA GAATATCACT 
TTCCTGATTC CTGGTTTCTA CCACTACATG GACCTCACAT ATGATAGTTC GGTACAGCTG 
CCAGATCTGG TCAATTGCCT CGACATTGGC AGAAAGAGTG GGCCTGGGGA GATGAACTCT 
GATGCCCAAC ACAACTGGGA ACTGGCCAGT GGGGCCCAGG GTCCAAGAAT TGACGTGGTG 
TTGGCACGGG ACCACATGCC ATCACAAGAT GAGACCCTGA AGCTGGTGGG AGGACAAGTC 
TCCTCTAGGA CTCAGACACG ATTGGAAACT GGATACAAGA TACTTCCTTC TTCTGCATGT 
CAGCCCTCAC TGGGGGTGGA CAGCAACCCA GAGGATGGTG AGCAGTCATT GCTCACAGAC 
CAGCAAAGCA TTCCAAAGAG AAACCTGCCA GCCCATGGTG GTCAGAACCA GCTAGACACC 
TCAGAAATAT CCCTACCAGT CCTTAAAGAA CATTTATTGT CTGAAATTCA GAAAAATATC 
TCAGAAGAAA GCCCACTAAC CCACAGGAAA TGGTTATCCA AGGTGCAACA AAACCCCACA 
AAAGGAAGCC TGCCCATCCA CGAGGAGCAG CTATTGTTTG CAGGCCAGCA TATCCATGTC 
TTGGGAAAGG AGGATCCTTC AGCAGCAGAG AGCTACAGGG AGAGTCTTTT AGAAGAAAGC 
AAGTCCAAAC CAGTGGATGC AAAACTGTTT GCTTCATGTG AAGATTCTGA GCCTCTAGTT 
TCAGTTAAGG ACAGGGGCAG CAGTGTGGAC ACGTGTAATC TGCATCAGGA AGCTCAGTTG 
GAGCTGATGG GTGTCAGCAG TCCTAACCCC TGGGCTGATG CCACCATGCC TGAGCCCCAC 
ACCACAGGGC AGATAGCAGG CGGGAGCCTT ACATATTGCC CACAGTACAG AAGCGAGTGG 
GCCTCACAGC AGCGAGGCCA GGACTCGGCT CCCAGCCCCT CTGGAATGGC GTGTGTCTTG 
CTTGGGACAC CCACTCTGGA TGAACCATGG CCAGGAGTGA GAAATGACAG AGAAGAGCTG 
CAGACTTGCT TGATTAAGGA ACAGTTATCC AAGTCAAGCT GTGAGGGAAA TCTGGGCATC 
TCCCGTGTGG AACTCGTTCC TGAAGAACAT CCTCCTTTCA CCGCCCCCGT GTCTTTCTGT 
GATCTGGGAG GCCGAGATCT ACACGCTAGC CGCTCAGGCA GCTCCTCAGC CTGCTATGCT 
TTGGCCACCG ACCTCCCTGG TGTCCTGGAG GCAGTGGAAG CTCAGGAGGC TGATGTGAAT 
TCTTATTCCT GGAACCTCAA GGAGCTCTTT CTCAAGGACC AGACAGACAG AACTCCATCA 
CATTGTTCCT GTACCACATC TGAACTCAGC GAGGCGCCAT CTCTTTCAGT GGTGGGGTCA 
GATTTAGATG TGGGTATTCT GCATAGGCAG ACTTCAGATA TTTTAGTTGA CCGAGAGATG 
TTGCTCCTTA CTGGGACCTA TTTTGACCTT AGTGAAGGCC AACGGTTCCA GGAGATGGGA 
GCAGGACATG ATCGAGCAGA ACTGTCTAAC ATTTCTTTGG TATCCTCTGA ACACTATGAA 
ACTAGTGACA TAGAAAGCCC AGGTTGTGAC CCTCCCTTAC CAGATCCTGG TCCCAACGAT 
ATGTGCCTAT CAGCAGAGAA GCCCAGGCCG AGTGCCCAGA TCACTTCCAC TCCTGTGGCA 
CGTGGGGCCA CCAGCCTGCA GCAGGAGATC CAAGAGGGCA TCTACTCTGG AAGCTGCTAC 
CACCGGGATG GCTTACAACT GAGTATACAG TTTGAAGTCA AGCGTGTGGA GCTCCAGGGC 
TCTGCGACCC TGTTCTGCTG CTGGCTGGTG AAGGACCTCT TCCATAGCCA CCGGGACTCA 
GCCACACGAA CCCGCCTGTT CCTTGCCAGT CTGCCTAGCT CTACCCACTC CATGCCTGAG 
CTGTCTGGAT CCAGCTTTGG AGAGGTGCTC AGAGCCAAGC CTTGGTTTGA GGAATCCCCA 
ACACCTGCAG AGCTAGAGGG GCTGGCGGCC TGTGAGGGCG AGTACGACTA TAAGTATAAC 
ACCATCAGCC CACTTGGCAG TGGGGCCTTT GGCTTTGTGT GGACTGCAGT GGAGAAGGAA 
TGCAACAAAG AGGTGGTGGT GAAGTTTATT AAGAAGGAGA AGGTTTTAGA GGATTGCTGG 
ATTGAGGATC CCAAACTTGG AAGAGTTACT TTAGAGATTG CAATTCTGTC CAAGGTGGAC 
CATGCCAACA TAATCAAGGT TCTGGATATA TTCGAAAACC AAGAGTTCTT CCAGCTGGTG 
ATGGAAAAGC ACGGCTCTGG CATGGACCTC TTTGCTTTCA TTGACCACCA TCCCTGCTTG 
GATGAGCCCC TGGCGAGTTT CATCTTTCGA CAACTAGTGT CTGCTGTAGG ATACCTGCAC 
TCCCAAGGCA TCATCCATAG AGACATCAAG GATGAGAACA TTGTGATTGC TGAGGACTTC 
ACAATTAAGC TGATAGATTT TGGCTCAGCT GCCTACTTAG AGAGGGGCAA ACTATTTTAT 
ACCTTTTGTG GAACAATCGA ATACTGTGCA CCTGAGGTTC TCATTGGAAA TCCCTACAGA 
GGGCCAGAGC TGGAGATGTG GTCTCTGGGG GTCACCCTGT ACACGCTCAT CTTCGAGGAG 
AATCCCTTCT GTGAGGTGGA GGAGACCATG GAGGCAGTTA TTCATCCCCC ATTCCTGGTT 
TCCCAAGAAC TTATGAGTCT TCTGTCTGGA CTGCTGCAGC CTTGCCCTGA GCAGCGGACC 
ACTTTGGAGA AGCTGATCAG GGACCCCTGG GTGACACAGC CTGTGAACCT TGCTAGCTAT 
ACTTGGGAAG AGGTGTGTAG GACCAACCAG CCAGAAAGTG GCCTGCTGTC AGCTGCAAGT 
CTGGAGATTG GGAGTAGGAG TCCAAGTGAA ATGGCTCAGA GAGAGGGTCT CTGTGGGCCT 
CCTGCTCCCA GGGAGACTCG TGGTGACCAG CACTGCTTGC ATCTTAAGGA CCCCTCTTTG 
CCAGTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pask ( XM_006529665.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529728.1 Mus musculus PAS domain containing serine/threonine kinase (Pask), transcript variant X1, mRNA.

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CloneID	OMu21506
Clone ID Related Accession (Same CDS sequence)	XM_006529665.2 , XM_006529665.4 , XM_006529666.2 , XM_006529666.3 , NM_080850.2 , XM_006529665.3 , XM_006529667.2 , XM_017321037.1
Accession Version	XM_006529665.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	PAS domain-containing serine/threonine-protein kinase isoform X1
Comment

ATGGAGGACA GAGGTCCACC AGTTTTTGCT GAGGACTGGA AATGTTTGTC TGAGAGTCCT 
CCTGTCCAAG AAGGCCCAGC GGCCCAGGCT ACCTTTGAGC CCAGCAAGCC ACTCTCCATA 
GCCCACAAAC ACCTGAGCAG GAAGAATGGG CTTTCTAGAC TCTGCCAGAG CCGAATGGCG 
CTCTCTGAGG ACAGGTGGAG CTCTTACTGT CTTTCATCAT TGGCTGCCCA AAACATCTGC 
ACAAGCAAAC TGCACTGCGC TGCTGCACCT GAGTATGCTG ACCCTACAGC TGGGCCACTG 
GGCAGTACTT CCTGCTGCTC CTTGCTGCGT GGCCTGGCTT CAGGATGCTC TGGGTCCCTG 
CTTTCGACCC CTGTGTGCAA CCCCAACAAG GCTGTCTTTA CCGTGGATGC CAAGACCACA 
GAGATTCTGG TTGCCAACGA CAAAGCCTGC AGCCTCCTAG GGTATAGCAG TCATGACCTG 
ATTGGCCAAA AGCTCGCCCA ATTCTTCCTC AAATCTGATT CCGAGGTGGT GGAAGCCCTC 
AGCGAGGAAC ATGTGGAGGC TGATGGCCAC GCTGCAGTGG TGTTCGGCAC AGTGGTGGAC 
ATCGTCAGCC GAATTGGGGA GAAGATTCCA GTCTCTGTGT GGATAAAGAG ATTGCAGCAA 
GATCGTGGCT TGTGCTGTGT AGTGGTTCTG GAGCCTGTGG AGAGAGTGTC AGCCTGGGTC 
GCCTTCCAGA GTGATGGAAC TATCACATCA TGCGACAGTC TCTTTGCTCA TCTCCATGGG 
TTCACATCTC CAAAGGATGT AGTTGGGCAG TGTGTCATAG ACTTGATTCC ATCCATGCAG 
CTCCCTCCTC CTGGCCAACA TATCCCAAAG AGTCTCAAGA TTCAAAGGTC TGTTGGGAGA 
GCCAGAGACG GCACCACCTT CCCTCTCAGT TTGAAGCTGA AATCCAAGCC CAGTGGAAGG 
GCTGTAGCTG ACAGTGAGGC GGCCTCTGAA CCAGGTTACC AGGCTTCTGT GTGGGTGTTC 
TGCACCATCA GTGGCCTCAT CACTCTGCTG CCAGATGGCA CAATTTATGG AGTCAACCAC 
AGCTTTGCAC TAATGCTGTT TGGTTATGGA AAGACAGAGC TCCTGGGCAA GAATATCACT 
TTCCTGATTC CTGGTTTCTA CCACTACATG GACCTCACAT ATGATAGTTC GGTACAGCTG 
CCAGATCTGG TCAATTGCCT CGACATTGGC AGAAAGAGTG GGCCTGGGGA GATGAACTCT 
GATGCCCAAC ACAACTGGGA ACTGGCCAGT GGGGCCCAGG GTCCAAGAAT TGACGTGGTG 
TTGGCACGGG ACCACATGCC ATCACAAGAT GAGACCCTGA AGCTGGTGGG AGGACAAGTC 
TCCTCTAGGA CTCAGACACG ATTGGAAACT GGATACAAGA TACTTCCTTC TTCTGCATGT 
CAGCCCTCAC TGGGGGTGGA CAGCAACCCA GAGGATGGTG AGCAGTCATT GCTCACAGAC 
CAGCAAAGCA TTCCAAAGAG AAACCTGCCA GCCCATGGTG GTCAGAACCA GCTAGACACC 
TCAGAAATAT CCCTACCAGT CCTTAAAGAA CATTTATTGT CTGAAATTCA GAAAAATATC 
TCAGAAGAAA GCCCACTAAC CCACAGGAAA TGGTTATCCA AGGTGCAACA AAACCCCACA 
AAAGGAAGCC TGCCCATCCA CGAGGAGCAG CTATTGTTTG CAGGCCAGCA TATCCATGTC 
TTGGGAAAGG AGGATCCTTC AGCAGCAGAG AGCTACAGGG AGAGTCTTTT AGAAGAAAGC 
AAGTCCAAAC CAGTGGATGC AAAACTGTTT GCTTCATGTG AAGATTCTGA GCCTCTAGTT 
TCAGTTAAGG ACAGGGGCAG CAGTGTGGAC ACGTGTAATC TGCATCAGGA AGCTCAGTTG 
GAGCTGATGG GTGTCAGCAG TCCTAACCCC TGGGCTGATG CCACCATGCC TGAGCCCCAC 
ACCACAGGGC AGATAGCAGG CGGGAGCCTT ACATATTGCC CACAGTACAG AAGCGAGTGG 
GCCTCACAGC AGCGAGGCCA GGACTCGGCT CCCAGCCCCT CTGGAATGGC GTGTGTCTTG 
CTTGGGACAC CCACTCTGGA TGAACCATGG CCAGGAGTGA GAAATGACAG AGAAGAGCTG 
CAGACTTGCT TGATTAAGGA ACAGTTATCC AAGTCAAGCT GTGAGGGAAA TCTGGGCATC 
TCCCGTGTGG AACTCGTTCC TGAAGAACAT CCTCCTTTCA CCGCCCCCGT GTCTTTCTGT 
GATCTGGGAG GCCGAGATCT ACACGCTAGC CGCTCAGGCA GCTCCTCAGC CTGCTATGCT 
TTGGCCACCG ACCTCCCTGG TGTCCTGGAG GCAGTGGAAG CTCAGGAGGC TGATGTGAAT 
TCTTATTCCT GGAACCTCAA GGAGCTCTTT CTCAAGGACC AGACAGACAG AACTCCATCA 
CATTGTTCCT GTACCACATC TGAACTCAGC GAGGCGCCAT CTCTTTCAGT GGTGGGGTCA 
GATTTAGATG TGGGTATTCT GCATAGGCAG ACTTCAGATA TTTTAGTTGA CCGAGAGATG 
TTGCTCCTTA CTGGGACCTA TTTTGACCTT AGTGAAGGCC AACGGTTCCA GGAGATGGGA 
GCAGGACATG ATCGAGCAGA ACTGTCTAAC ATTTCTTTGG TATCCTCTGA ACACTATGAA 
ACTAGTGACA TAGAAAGCCC AGGTTGTGAC CCTCCCTTAC CAGATCCTGG TCCCAACGAT 
ATGTGCCTAT CAGCAGAGAA GCCCAGGCCG AGTGCCCAGA TCACTTCCAC TCCTGTGGCA 
CGTGGGGCCA CCAGCCTGCA GCAGGAGATC CAAGAGGGCA TCTACTCTGG AAGCTGCTAC 
CACCGGGATG GCTTACAACT GAGTATACAG TTTGAAGTCA AGCGTGTGGA GCTCCAGGGC 
TCTGCGACCC TGTTCTGCTG CTGGCTGGTG AAGGACCTCT TCCATAGCCA CCGGGACTCA 
GCCACACGAA CCCGCCTGTT CCTTGCCAGT CTGCCTAGCT CTACCCACTC CATGCCTGAG 
CTGTCTGGAT CCAGCTTTGG AGAGGTGCTC AGAGCCAAGC CTTGGTTTGA GGAATCCCCA 
ACACCTGCAG AGCTAGAGGG GCTGGCGGCC TGTGAGGGCG AGTACGACTA TAAGTATAAC 
ACCATCAGCC CACTTGGCAG TGGGGCCTTT GGCTTTGTGT GGACTGCAGT GGAGAAGGAA 
TGCAACAAAG AGGTGGTGGT GAAGTTTATT AAGAAGGAGA AGGTTTTAGA GGATTGCTGG 
ATTGAGGATC CCAAACTTGG AAGAGTTACT TTAGAGATTG CAATTCTGTC CAAGGTGGAC 
CATGCCAACA TAATCAAGGT TCTGGATATA TTCGAAAACC AAGAGTTCTT CCAGCTGGTG 
ATGGAAAAGC ACGGCTCTGG CATGGACCTC TTTGCTTTCA TTGACCACCA TCCCTGCTTG 
GATGAGCCCC TGGCGAGTTT CATCTTTCGA CAACTAGTGT CTGCTGTAGG ATACCTGCAC 
TCCCAAGGCA TCATCCATAG AGACATCAAG GATGAGAACA TTGTGATTGC TGAGGACTTC 
ACAATTAAGC TGATAGATTT TGGCTCAGCT GCCTACTTAG AGAGGGGCAA ACTATTTTAT 
ACCTTTTGTG GAACAATCGA ATACTGTGCA CCTGAGGTTC TCATTGGAAA TCCCTACAGA 
GGGCCAGAGC TGGAGATGTG GTCTCTGGGG GTCACCCTGT ACACGCTCAT CTTCGAGGAG 
AATCCCTTCT GTGAGGTGGA GGAGACCATG GAGGCAGTTA TTCATCCCCC ATTCCTGGTT 
TCCCAAGAAC TTATGAGTCT TCTGTCTGGA CTGCTGCAGC CTTGCCCTGA GCAGCGGACC 
ACTTTGGAGA AGCTGATCAG GGACCCCTGG GTGACACAGC CTGTGAACCT TGCTAGCTAT 
ACTTGGGAAG AGGTGTGTAG GACCAACCAG CCAGAAAGTG GCCTGCTGTC AGCTGCAAGT 
CTGGAGATTG GGAGTAGGAG TCCAAGTGAA ATGGCTCAGA GAGAGGGTCT CTGTGGGCCT 
CCTGCTCCCA GGGAGACTCG TGGTGACCAG CACTGCTTGC ATCTTAAGGA CCCCTCTTTG 
CCAGTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006529728.1

Translation

MEDRGPPVFAEDWKCLSESPPVQEGPAAQATFEPSKPLSIAHKHLSRKNGLSRLCQSRMALSEDRWSSYCLSSLAAQNICTSKLHCAAAPEYADPTAGPLGSTSCCSLLRGLASGCSGSLLSTPVCNPNKAVFTVDAKTTEILVANDKACSLLGYSSHDLIGQKLAQFFLKSDSEVVEALSEEHVEADGHAAVVFGTVVDIVSRIGEKIPVSVWIKRLQQDRGLCCVVVLEPVERVSAWVAFQSDGTITSCDSLFAHLHGFTSPKDVVGQCVIDLIPSMQLPPPGQHIPKSLKIQRSVGRARDGTTFPLSLKLKSKPSGRAVADSEAASEPGYQASVWVFCTISGLITLLPDGTIYGVNHSFALMLFGYGKTELLGKNITFLIPGFYHYMDLTYDSSVQLPDLVNCLDIGRKSGPGEMNSDAQHNWELASGAQGPRIDVVLARDHMPSQDETLKLVGGQVSSRTQTRLETGYKILPSSACQPSLGVDSNPEDGEQSLLTDQQSIPKRNLPAHGGQNQLDTSEISLPVLKEHLLSEIQKNISEESPLTHRKWLSKVQQNPTKGSLPIHEEQLLFAGQHIHVLGKEDPSAAESYRESLLEESKSKPVDAKLFASCEDSEPLVSVKDRGSSVDTCNLHQEAQLELMGVSSPNPWADATMPEPHTTGQIAGGSLTYCPQYRSEWASQQRGQDSAPSPSGMACVLLGTPTLDEPWPGVRNDREELQTCLIKEQLSKSSCEGNLGISRVELVPEEHPPFTAPVSFCDLGGRDLHASRSGSSSACYALATDLPGVLEAVEAQEADVNSYSWNLKELFLKDQTDRTPSHCSCTTSELSEAPSLSVVGSDLDVGILHRQTSDILVDREMLLLTGTYFDLSEGQRFQEMGAGHDRAELSNISLVSSEHYETSDIESPGCDPPLPDPGPNDMCLSAEKPRPSAQITSTPVARGATSLQQEIQEGIYSGSCYHRDGLQLSIQFEVKRVELQGSATLFCCWLVKDLFHSHRDSATRTRLFLASLPSSTHSMPELSGSSFGEVLRAKPWFEESPTPAELEGLAACEGEYDYKYNTISPLGSGAFGFVWTAVEKECNKEVVVKFIKKEKVLEDCWIEDPKLGRVTLEIAILSKVDHANIIKVLDIFENQEFFQLVMEKHGSGMDLFAFIDHHPCLDEPLASFIFRQLVSAVGYLHSQGIIHRDIKDENIVIAEDFTIKLIDFGSAAYLERGKLFYTFCGTIEYCAPEVLIGNPYRGPELEMWSLGVTLYTLIFEENPFCEVEETMEAVIHPPFLVSQELMSLLSGLLQPCPEQRTTLEKLIRDPWVTQPVNLASYTWEEVCRTNQPESGLLSAASLEIGSRSPSEMAQREGLCGPPAPRETRGDQHCLHLKDPSLPVS

Target ORF information:

RefSeq Version	XM_006529665.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus PAS domain containing serine/threonine kinase (Pask), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529665.2

ORF Insert Sequence:

ATGGAGGACA GAGGTCCACC AGTTTTTGCT GAGGACTGGA AATGTTTGTC TGAGAGTCCT 
CCTGTCCAAG AAGGCCCAGC GGCCCAGGCT ACCTTTGAGC CCAGCAAGCC ACTCTCCATA 
GCCCACAAAC ACCTGAGCAG GAAGAATGGG CTTTCTAGAC TCTGCCAGAG CCGAATGGCG 
CTCTCTGAGG ACAGGTGGAG CTCTTACTGT CTTTCATCAT TGGCTGCCCA AAACATCTGC 
ACAAGCAAAC TGCACTGCGC TGCTGCACCT GAGTATGCTG ACCCTACAGC TGGGCCACTG 
GGCAGTACTT CCTGCTGCTC CTTGCTGCGT GGCCTGGCTT CAGGATGCTC TGGGTCCCTG 
CTTTCGACCC CTGTGTGCAA CCCCAACAAG GCTGTCTTTA CCGTGGATGC CAAGACCACA 
GAGATTCTGG TTGCCAACGA CAAAGCCTGC AGCCTCCTAG GGTATAGCAG TCATGACCTG 
ATTGGCCAAA AGCTCGCCCA ATTCTTCCTC AAATCTGATT CCGAGGTGGT GGAAGCCCTC 
AGCGAGGAAC ATGTGGAGGC TGATGGCCAC GCTGCAGTGG TGTTCGGCAC AGTGGTGGAC 
ATCGTCAGCC GAATTGGGGA GAAGATTCCA GTCTCTGTGT GGATAAAGAG ATTGCAGCAA 
GATCGTGGCT TGTGCTGTGT AGTGGTTCTG GAGCCTGTGG AGAGAGTGTC AGCCTGGGTC 
GCCTTCCAGA GTGATGGAAC TATCACATCA TGCGACAGTC TCTTTGCTCA TCTCCATGGG 
TTCACATCTC CAAAGGATGT AGTTGGGCAG TGTGTCATAG ACTTGATTCC ATCCATGCAG 
CTCCCTCCTC CTGGCCAACA TATCCCAAAG AGTCTCAAGA TTCAAAGGTC TGTTGGGAGA 
GCCAGAGACG GCACCACCTT CCCTCTCAGT TTGAAGCTGA AATCCAAGCC CAGTGGAAGG 
GCTGTAGCTG ACAGTGAGGC GGCCTCTGAA CCAGGTTACC AGGCTTCTGT GTGGGTGTTC 
TGCACCATCA GTGGCCTCAT CACTCTGCTG CCAGATGGCA CAATTTATGG AGTCAACCAC 
AGCTTTGCAC TAATGCTGTT TGGTTATGGA AAGACAGAGC TCCTGGGCAA GAATATCACT 
TTCCTGATTC CTGGTTTCTA CCACTACATG GACCTCACAT ATGATAGTTC GGTACAGCTG 
CCAGATCTGG TCAATTGCCT CGACATTGGC AGAAAGAGTG GGCCTGGGGA GATGAACTCT 
GATGCCCAAC ACAACTGGGA ACTGGCCAGT GGGGCCCAGG GTCCAAGAAT TGACGTGGTG 
TTGGCACGGG ACCACATGCC ATCACAAGAT GAGACCCTGA AGCTGGTGGG AGGACAAGTC 
TCCTCTAGGA CTCAGACACG ATTGGAAACT GGATACAAGA TACTTCCTTC TTCTGCATGT 
CAGCCCTCAC TGGGGGTGGA CAGCAACCCA GAGGATGGTG AGCAGTCATT GCTCACAGAC 
CAGCAAAGCA TTCCAAAGAG AAACCTGCCA GCCCATGGTG GTCAGAACCA GCTAGACACC 
TCAGAAATAT CCCTACCAGT CCTTAAAGAA CATTTATTGT CTGAAATTCA GAAAAATATC 
TCAGAAGAAA GCCCACTAAC CCACAGGAAA TGGTTATCCA AGGTGCAACA AAACCCCACA 
AAAGGAAGCC TGCCCATCCA CGAGGAGCAG CTATTGTTTG CAGGCCAGCA TATCCATGTC 
TTGGGAAAGG AGGATCCTTC AGCAGCAGAG AGCTACAGGG AGAGTCTTTT AGAAGAAAGC 
AAGTCCAAAC CAGTGGATGC AAAACTGTTT GCTTCATGTG AAGATTCTGA GCCTCTAGTT 
TCAGTTAAGG ACAGGGGCAG CAGTGTGGAC ACGTGTAATC TGCATCAGGA AGCTCAGTTG 
GAGCTGATGG GTGTCAGCAG TCCTAACCCC TGGGCTGATG CCACCATGCC TGAGCCCCAC 
ACCACAGGGC AGATAGCAGG CGGGAGCCTT ACATATTGCC CACAGTACAG AAGCGAGTGG 
GCCTCACAGC AGCGAGGCCA GGACTCGGCT CCCAGCCCCT CTGGAATGGC GTGTGTCTTG 
CTTGGGACAC CCACTCTGGA TGAACCATGG CCAGGAGTGA GAAATGACAG AGAAGAGCTG 
CAGACTTGCT TGATTAAGGA ACAGTTATCC AAGTCAAGCT GTGAGGGAAA TCTGGGCATC 
TCCCGTGTGG AACTCGTTCC TGAAGAACAT CCTCCTTTCA CCGCCCCCGT GTCTTTCTGT 
GATCTGGGAG GCCGAGATCT ACACGCTAGC CGCTCAGGCA GCTCCTCAGC CTGCTATGCT 
TTGGCCACCG ACCTCCCTGG TGTCCTGGAG GCAGTGGAAG CTCAGGAGGC TGATGTGAAT 
TCTTATTCCT GGAACCTCAA GGAGCTCTTT CTCAAGGACC AGACAGACAG AACTCCATCA 
CATTGTTCCT GTACCACATC TGAACTCAGC GAGGCGCCAT CTCTTTCAGT GGTGGGGTCA 
GATTTAGATG TGGGTATTCT GCATAGGCAG ACTTCAGATA TTTTAGTTGA CCGAGAGATG 
TTGCTCCTTA CTGGGACCTA TTTTGACCTT AGTGAAGGCC AACGGTTCCA GGAGATGGGA 
GCAGGACATG ATCGAGCAGA ACTGTCTAAC ATTTCTTTGG TATCCTCTGA ACACTATGAA 
ACTAGTGACA TAGAAAGCCC AGGTTGTGAC CCTCCCTTAC CAGATCCTGG TCCCAACGAT 
ATGTGCCTAT CAGCAGAGAA GCCCAGGCCG AGTGCCCAGA TCACTTCCAC TCCTGTGGCA 
CGTGGGGCCA CCAGCCTGCA GCAGGAGATC CAAGAGGGCA TCTACTCTGG AAGCTGCTAC 
CACCGGGATG GCTTACAACT GAGTATACAG TTTGAAGTCA AGCGTGTGGA GCTCCAGGGC 
TCTGCGACCC TGTTCTGCTG CTGGCTGGTG AAGGACCTCT TCCATAGCCA CCGGGACTCA 
GCCACACGAA CCCGCCTGTT CCTTGCCAGT CTGCCTAGCT CTACCCACTC CATGCCTGAG 
CTGTCTGGAT CCAGCTTTGG AGAGGTGCTC AGAGCCAAGC CTTGGTTTGA GGAATCCCCA 
ACACCTGCAG AGCTAGAGGG GCTGGCGGCC TGTGAGGGCG AGTACGACTA TAAGTATAAC 
ACCATCAGCC CACTTGGCAG TGGGGCCTTT GGCTTTGTGT GGACTGCAGT GGAGAAGGAA 
TGCAACAAAG AGGTGGTGGT GAAGTTTATT AAGAAGGAGA AGGTTTTAGA GGATTGCTGG 
ATTGAGGATC CCAAACTTGG AAGAGTTACT TTAGAGATTG CAATTCTGTC CAAGGTGGAC 
CATGCCAACA TAATCAAGGT TCTGGATATA TTCGAAAACC AAGAGTTCTT CCAGCTGGTG 
ATGGAAAAGC ACGGCTCTGG CATGGACCTC TTTGCTTTCA TTGACCACCA TCCCTGCTTG 
GATGAGCCCC TGGCGAGTTT CATCTTTCGA CAACTAGTGT CTGCTGTAGG ATACCTGCAC 
TCCCAAGGCA TCATCCATAG AGACATCAAG GATGAGAACA TTGTGATTGC TGAGGACTTC 
ACAATTAAGC TGATAGATTT TGGCTCAGCT GCCTACTTAG AGAGGGGCAA ACTATTTTAT 
ACCTTTTGTG GAACAATCGA ATACTGTGCA CCTGAGGTTC TCATTGGAAA TCCCTACAGA 
GGGCCAGAGC TGGAGATGTG GTCTCTGGGG GTCACCCTGT ACACGCTCAT CTTCGAGGAG 
AATCCCTTCT GTGAGGTGGA GGAGACCATG GAGGCAGTTA TTCATCCCCC ATTCCTGGTT 
TCCCAAGAAC TTATGAGTCT TCTGTCTGGA CTGCTGCAGC CTTGCCCTGA GCAGCGGACC 
ACTTTGGAGA AGCTGATCAG GGACCCCTGG GTGACACAGC CTGTGAACCT TGCTAGCTAT 
ACTTGGGAAG AGGTGTGTAG GACCAACCAG CCAGAAAGTG GCCTGCTGTC AGCTGCAAGT 
CTGGAGATTG GGAGTAGGAG TCCAAGTGAA ATGGCTCAGA GAGAGGGTCT CTGTGGGCCT 
CCTGCTCCCA GGGAGACTCG TGGTGACCAG CACTGCTTGC ATCTTAAGGA CCCCTCTTTG 
CCAGTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pask ( XM_006529666.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529729.1 Mus musculus PAS domain containing serine/threonine kinase (Pask), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu21506
Clone ID Related Accession (Same CDS sequence)	XM_006529665.2 , XM_006529665.4 , XM_006529666.2 , XM_006529666.3 , NM_080850.2 , XM_006529665.3 , XM_006529667.2 , XM_017321037.1
Accession Version	XM_006529666.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	PAS domain-containing serine/threonine-protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006529666.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GAGGTCCACC AGTTTTTGCT GAGGACTGGA AATGTTTGTC TGAGAGTCCT 
CCTGTCCAAG AAGGCCCAGC GGCCCAGGCT ACCTTTGAGC CCAGCAAGCC ACTCTCCATA 
GCCCACAAAC ACCTGAGCAG GAAGAATGGG CTTTCTAGAC TCTGCCAGAG CCGAATGGCG 
CTCTCTGAGG ACAGGTGGAG CTCTTACTGT CTTTCATCAT TGGCTGCCCA AAACATCTGC 
ACAAGCAAAC TGCACTGCGC TGCTGCACCT GAGTATGCTG ACCCTACAGC TGGGCCACTG 
GGCAGTACTT CCTGCTGCTC CTTGCTGCGT GGCCTGGCTT CAGGATGCTC TGGGTCCCTG 
CTTTCGACCC CTGTGTGCAA CCCCAACAAG GCTGTCTTTA CCGTGGATGC CAAGACCACA 
GAGATTCTGG TTGCCAACGA CAAAGCCTGC AGCCTCCTAG GGTATAGCAG TCATGACCTG 
ATTGGCCAAA AGCTCGCCCA ATTCTTCCTC AAATCTGATT CCGAGGTGGT GGAAGCCCTC 
AGCGAGGAAC ATGTGGAGGC TGATGGCCAC GCTGCAGTGG TGTTCGGCAC AGTGGTGGAC 
ATCGTCAGCC GAATTGGGGA GAAGATTCCA GTCTCTGTGT GGATAAAGAG ATTGCAGCAA 
GATCGTGGCT TGTGCTGTGT AGTGGTTCTG GAGCCTGTGG AGAGAGTGTC AGCCTGGGTC 
GCCTTCCAGA GTGATGGAAC TATCACATCA TGCGACAGTC TCTTTGCTCA TCTCCATGGG 
TTCACATCTC CAAAGGATGT AGTTGGGCAG TGTGTCATAG ACTTGATTCC ATCCATGCAG 
CTCCCTCCTC CTGGCCAACA TATCCCAAAG AGTCTCAAGA TTCAAAGGTC TGTTGGGAGA 
GCCAGAGACG GCACCACCTT CCCTCTCAGT TTGAAGCTGA AATCCAAGCC CAGTGGAAGG 
GCTGTAGCTG ACAGTGAGGC GGCCTCTGAA CCAGGTTACC AGGCTTCTGT GTGGGTGTTC 
TGCACCATCA GTGGCCTCAT CACTCTGCTG CCAGATGGCA CAATTTATGG AGTCAACCAC 
AGCTTTGCAC TAATGCTGTT TGGTTATGGA AAGACAGAGC TCCTGGGCAA GAATATCACT 
TTCCTGATTC CTGGTTTCTA CCACTACATG GACCTCACAT ATGATAGTTC GGTACAGCTG 
CCAGATCTGG TCAATTGCCT CGACATTGGC AGAAAGAGTG GGCCTGGGGA GATGAACTCT 
GATGCCCAAC ACAACTGGGA ACTGGCCAGT GGGGCCCAGG GTCCAAGAAT TGACGTGGTG 
TTGGCACGGG ACCACATGCC ATCACAAGAT GAGACCCTGA AGCTGGTGGG AGGACAAGTC 
TCCTCTAGGA CTCAGACACG ATTGGAAACT GGATACAAGA TACTTCCTTC TTCTGCATGT 
CAGCCCTCAC TGGGGGTGGA CAGCAACCCA GAGGATGGTG AGCAGTCATT GCTCACAGAC 
CAGCAAAGCA TTCCAAAGAG AAACCTGCCA GCCCATGGTG GTCAGAACCA GCTAGACACC 
TCAGAAATAT CCCTACCAGT CCTTAAAGAA CATTTATTGT CTGAAATTCA GAAAAATATC 
TCAGAAGAAA GCCCACTAAC CCACAGGAAA TGGTTATCCA AGGTGCAACA AAACCCCACA 
AAAGGAAGCC TGCCCATCCA CGAGGAGCAG CTATTGTTTG CAGGCCAGCA TATCCATGTC 
TTGGGAAAGG AGGATCCTTC AGCAGCAGAG AGCTACAGGG AGAGTCTTTT AGAAGAAAGC 
AAGTCCAAAC CAGTGGATGC AAAACTGTTT GCTTCATGTG AAGATTCTGA GCCTCTAGTT 
TCAGTTAAGG ACAGGGGCAG CAGTGTGGAC ACGTGTAATC TGCATCAGGA AGCTCAGTTG 
GAGCTGATGG GTGTCAGCAG TCCTAACCCC TGGGCTGATG CCACCATGCC TGAGCCCCAC 
ACCACAGGGC AGATAGCAGG CGGGAGCCTT ACATATTGCC CACAGTACAG AAGCGAGTGG 
GCCTCACAGC AGCGAGGCCA GGACTCGGCT CCCAGCCCCT CTGGAATGGC GTGTGTCTTG 
CTTGGGACAC CCACTCTGGA TGAACCATGG CCAGGAGTGA GAAATGACAG AGAAGAGCTG 
CAGACTTGCT TGATTAAGGA ACAGTTATCC AAGTCAAGCT GTGAGGGAAA TCTGGGCATC 
TCCCGTGTGG AACTCGTTCC TGAAGAACAT CCTCCTTTCA CCGCCCCCGT GTCTTTCTGT 
GATCTGGGAG GCCGAGATCT ACACGCTAGC CGCTCAGGCA GCTCCTCAGC CTGCTATGCT 
TTGGCCACCG ACCTCCCTGG TGTCCTGGAG GCAGTGGAAG CTCAGGAGGC TGATGTGAAT 
TCTTATTCCT GGAACCTCAA GGAGCTCTTT CTCAAGGACC AGACAGACAG AACTCCATCA 
CATTGTTCCT GTACCACATC TGAACTCAGC GAGGCGCCAT CTCTTTCAGT GGTGGGGTCA 
GATTTAGATG TGGGTATTCT GCATAGGCAG ACTTCAGATA TTTTAGTTGA CCGAGAGATG 
TTGCTCCTTA CTGGGACCTA TTTTGACCTT AGTGAAGGCC AACGGTTCCA GGAGATGGGA 
GCAGGACATG ATCGAGCAGA ACTGTCTAAC ATTTCTTTGG TATCCTCTGA ACACTATGAA 
ACTAGTGACA TAGAAAGCCC AGGTTGTGAC CCTCCCTTAC CAGATCCTGG TCCCAACGAT 
ATGTGCCTAT CAGCAGAGAA GCCCAGGCCG AGTGCCCAGA TCACTTCCAC TCCTGTGGCA 
CGTGGGGCCA CCAGCCTGCA GCAGGAGATC CAAGAGGGCA TCTACTCTGG AAGCTGCTAC 
CACCGGGATG GCTTACAACT GAGTATACAG TTTGAAGTCA AGCGTGTGGA GCTCCAGGGC 
TCTGCGACCC TGTTCTGCTG CTGGCTGGTG AAGGACCTCT TCCATAGCCA CCGGGACTCA 
GCCACACGAA CCCGCCTGTT CCTTGCCAGT CTGCCTAGCT CTACCCACTC CATGCCTGAG 
CTGTCTGGAT CCAGCTTTGG AGAGGTGCTC AGAGCCAAGC CTTGGTTTGA GGAATCCCCA 
ACACCTGCAG AGCTAGAGGG GCTGGCGGCC TGTGAGGGCG AGTACGACTA TAAGTATAAC 
ACCATCAGCC CACTTGGCAG TGGGGCCTTT GGCTTTGTGT GGACTGCAGT GGAGAAGGAA 
TGCAACAAAG AGGTGGTGGT GAAGTTTATT AAGAAGGAGA AGGTTTTAGA GGATTGCTGG 
ATTGAGGATC CCAAACTTGG AAGAGTTACT TTAGAGATTG CAATTCTGTC CAAGGTGGAC 
CATGCCAACA TAATCAAGGT TCTGGATATA TTCGAAAACC AAGAGTTCTT CCAGCTGGTG 
ATGGAAAAGC ACGGCTCTGG CATGGACCTC TTTGCTTTCA TTGACCACCA TCCCTGCTTG 
GATGAGCCCC TGGCGAGTTT CATCTTTCGA CAACTAGTGT CTGCTGTAGG ATACCTGCAC 
TCCCAAGGCA TCATCCATAG AGACATCAAG GATGAGAACA TTGTGATTGC TGAGGACTTC 
ACAATTAAGC TGATAGATTT TGGCTCAGCT GCCTACTTAG AGAGGGGCAA ACTATTTTAT 
ACCTTTTGTG GAACAATCGA ATACTGTGCA CCTGAGGTTC TCATTGGAAA TCCCTACAGA 
GGGCCAGAGC TGGAGATGTG GTCTCTGGGG GTCACCCTGT ACACGCTCAT CTTCGAGGAG 
AATCCCTTCT GTGAGGTGGA GGAGACCATG GAGGCAGTTA TTCATCCCCC ATTCCTGGTT 
TCCCAAGAAC TTATGAGTCT TCTGTCTGGA CTGCTGCAGC CTTGCCCTGA GCAGCGGACC 
ACTTTGGAGA AGCTGATCAG GGACCCCTGG GTGACACAGC CTGTGAACCT TGCTAGCTAT 
ACTTGGGAAG AGGTGTGTAG GACCAACCAG CCAGAAAGTG GCCTGCTGTC AGCTGCAAGT 
CTGGAGATTG GGAGTAGGAG TCCAAGTGAA ATGGCTCAGA GAGAGGGTCT CTGTGGGCCT 
CCTGCTCCCA GGGAGACTCG TGGTGACCAG CACTGCTTGC ATCTTAAGGA CCCCTCTTTG 
CCAGTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529729.1
CDS	135..4286
Translation	MEDRGPPVFAEDWKCLSESPPVQEGPAAQATFEPSKPLSIAHKHLSRKNGLSRLCQSRMALSEDRWSSYCLSSLAAQNICTSKLHCAAAPEYADPTAGPLGSTSCCSLLRGLASGCSGSLLSTPVCNPNKAVFTVDAKTTEILVANDKACSLLGYSSHDLIGQKLAQFFLKSDSEVVEALSEEHVEADGHAAVVFGTVVDIVSRIGEKIPVSVWIKRLQQDRGLCCVVVLEPVERVSAWVAFQSDGTITSCDSLFAHLHGFTSPKDVVGQCVIDLIPSMQLPPPGQHIPKSLKIQRSVGRARDGTTFPLSLKLKSKPSGRAVADSEAASEPGYQASVWVFCTISGLITLLPDGTIYGVNHSFALMLFGYGKTELLGKNITFLIPGFYHYMDLTYDSSVQLPDLVNCLDIGRKSGPGEMNSDAQHNWELASGAQGPRIDVVLARDHMPSQDETLKLVGGQVSSRTQTRLETGYKILPSSACQPSLGVDSNPEDGEQSLLTDQQSIPKRNLPAHGGQNQLDTSEISLPVLKEHLLSEIQKNISEESPLTHRKWLSKVQQNPTKGSLPIHEEQLLFAGQHIHVLGKEDPSAAESYRESLLEESKSKPVDAKLFASCEDSEPLVSVKDRGSSVDTCNLHQEAQLELMGVSSPNPWADATMPEPHTTGQIAGGSLTYCPQYRSEWASQQRGQDSAPSPSGMACVLLGTPTLDEPWPGVRNDREELQTCLIKEQLSKSSCEGNLGISRVELVPEEHPPFTAPVSFCDLGGRDLHASRSGSSSACYALATDLPGVLEAVEAQEADVNSYSWNLKELFLKDQTDRTPSHCSCTTSELSEAPSLSVVGSDLDVGILHRQTSDILVDREMLLLTGTYFDLSEGQRFQEMGAGHDRAELSNISLVSSEHYETSDIESPGCDPPLPDPGPNDMCLSAEKPRPSAQITSTPVARGATSLQQEIQEGIYSGSCYHRDGLQLSIQFEVKRVELQGSATLFCCWLVKDLFHSHRDSATRTRLFLASLPSSTHSMPELSGSSFGEVLRAKPWFEESPTPAELEGLAACEGEYDYKYNTISPLGSGAFGFVWTAVEKECNKEVVVKFIKKEKVLEDCWIEDPKLGRVTLEIAILSKVDHANIIKVLDIFENQEFFQLVMEKHGSGMDLFAFIDHHPCLDEPLASFIFRQLVSAVGYLHSQGIIHRDIKDENIVIAEDFTIKLIDFGSAAYLERGKLFYTFCGTIEYCAPEVLIGNPYRGPELEMWSLGVTLYTLIFEENPFCEVEETMEAVIHPPFLVSQELMSLLSGLLQPCPEQRTTLEKLIRDPWVTQPVNLASYTWEEVCRTNQPESGLLSAASLEIGSRSPSEMAQREGLCGPPAPRETRGDQHCLHLKDPSLPVS

Target ORF information:

RefSeq Version	XM_006529666.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus PAS domain containing serine/threonine kinase (Pask), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529666.2

ORF Insert Sequence:

ATGGAGGACA GAGGTCCACC AGTTTTTGCT GAGGACTGGA AATGTTTGTC TGAGAGTCCT 
CCTGTCCAAG AAGGCCCAGC GGCCCAGGCT ACCTTTGAGC CCAGCAAGCC ACTCTCCATA 
GCCCACAAAC ACCTGAGCAG GAAGAATGGG CTTTCTAGAC TCTGCCAGAG CCGAATGGCG 
CTCTCTGAGG ACAGGTGGAG CTCTTACTGT CTTTCATCAT TGGCTGCCCA AAACATCTGC 
ACAAGCAAAC TGCACTGCGC TGCTGCACCT GAGTATGCTG ACCCTACAGC TGGGCCACTG 
GGCAGTACTT CCTGCTGCTC CTTGCTGCGT GGCCTGGCTT CAGGATGCTC TGGGTCCCTG 
CTTTCGACCC CTGTGTGCAA CCCCAACAAG GCTGTCTTTA CCGTGGATGC CAAGACCACA 
GAGATTCTGG TTGCCAACGA CAAAGCCTGC AGCCTCCTAG GGTATAGCAG TCATGACCTG 
ATTGGCCAAA AGCTCGCCCA ATTCTTCCTC AAATCTGATT CCGAGGTGGT GGAAGCCCTC 
AGCGAGGAAC ATGTGGAGGC TGATGGCCAC GCTGCAGTGG TGTTCGGCAC AGTGGTGGAC 
ATCGTCAGCC GAATTGGGGA GAAGATTCCA GTCTCTGTGT GGATAAAGAG ATTGCAGCAA 
GATCGTGGCT TGTGCTGTGT AGTGGTTCTG GAGCCTGTGG AGAGAGTGTC AGCCTGGGTC 
GCCTTCCAGA GTGATGGAAC TATCACATCA TGCGACAGTC TCTTTGCTCA TCTCCATGGG 
TTCACATCTC CAAAGGATGT AGTTGGGCAG TGTGTCATAG ACTTGATTCC ATCCATGCAG 
CTCCCTCCTC CTGGCCAACA TATCCCAAAG AGTCTCAAGA TTCAAAGGTC TGTTGGGAGA 
GCCAGAGACG GCACCACCTT CCCTCTCAGT TTGAAGCTGA AATCCAAGCC CAGTGGAAGG 
GCTGTAGCTG ACAGTGAGGC GGCCTCTGAA CCAGGTTACC AGGCTTCTGT GTGGGTGTTC 
TGCACCATCA GTGGCCTCAT CACTCTGCTG CCAGATGGCA CAATTTATGG AGTCAACCAC 
AGCTTTGCAC TAATGCTGTT TGGTTATGGA AAGACAGAGC TCCTGGGCAA GAATATCACT 
TTCCTGATTC CTGGTTTCTA CCACTACATG GACCTCACAT ATGATAGTTC GGTACAGCTG 
CCAGATCTGG TCAATTGCCT CGACATTGGC AGAAAGAGTG GGCCTGGGGA GATGAACTCT 
GATGCCCAAC ACAACTGGGA ACTGGCCAGT GGGGCCCAGG GTCCAAGAAT TGACGTGGTG 
TTGGCACGGG ACCACATGCC ATCACAAGAT GAGACCCTGA AGCTGGTGGG AGGACAAGTC 
TCCTCTAGGA CTCAGACACG ATTGGAAACT GGATACAAGA TACTTCCTTC TTCTGCATGT 
CAGCCCTCAC TGGGGGTGGA CAGCAACCCA GAGGATGGTG AGCAGTCATT GCTCACAGAC 
CAGCAAAGCA TTCCAAAGAG AAACCTGCCA GCCCATGGTG GTCAGAACCA GCTAGACACC 
TCAGAAATAT CCCTACCAGT CCTTAAAGAA CATTTATTGT CTGAAATTCA GAAAAATATC 
TCAGAAGAAA GCCCACTAAC CCACAGGAAA TGGTTATCCA AGGTGCAACA AAACCCCACA 
AAAGGAAGCC TGCCCATCCA CGAGGAGCAG CTATTGTTTG CAGGCCAGCA TATCCATGTC 
TTGGGAAAGG AGGATCCTTC AGCAGCAGAG AGCTACAGGG AGAGTCTTTT AGAAGAAAGC 
AAGTCCAAAC CAGTGGATGC AAAACTGTTT GCTTCATGTG AAGATTCTGA GCCTCTAGTT 
TCAGTTAAGG ACAGGGGCAG CAGTGTGGAC ACGTGTAATC TGCATCAGGA AGCTCAGTTG 
GAGCTGATGG GTGTCAGCAG TCCTAACCCC TGGGCTGATG CCACCATGCC TGAGCCCCAC 
ACCACAGGGC AGATAGCAGG CGGGAGCCTT ACATATTGCC CACAGTACAG AAGCGAGTGG 
GCCTCACAGC AGCGAGGCCA GGACTCGGCT CCCAGCCCCT CTGGAATGGC GTGTGTCTTG 
CTTGGGACAC CCACTCTGGA TGAACCATGG CCAGGAGTGA GAAATGACAG AGAAGAGCTG 
CAGACTTGCT TGATTAAGGA ACAGTTATCC AAGTCAAGCT GTGAGGGAAA TCTGGGCATC 
TCCCGTGTGG AACTCGTTCC TGAAGAACAT CCTCCTTTCA CCGCCCCCGT GTCTTTCTGT 
GATCTGGGAG GCCGAGATCT ACACGCTAGC CGCTCAGGCA GCTCCTCAGC CTGCTATGCT 
TTGGCCACCG ACCTCCCTGG TGTCCTGGAG GCAGTGGAAG CTCAGGAGGC TGATGTGAAT 
TCTTATTCCT GGAACCTCAA GGAGCTCTTT CTCAAGGACC AGACAGACAG AACTCCATCA 
CATTGTTCCT GTACCACATC TGAACTCAGC GAGGCGCCAT CTCTTTCAGT GGTGGGGTCA 
GATTTAGATG TGGGTATTCT GCATAGGCAG ACTTCAGATA TTTTAGTTGA CCGAGAGATG 
TTGCTCCTTA CTGGGACCTA TTTTGACCTT AGTGAAGGCC AACGGTTCCA GGAGATGGGA 
GCAGGACATG ATCGAGCAGA ACTGTCTAAC ATTTCTTTGG TATCCTCTGA ACACTATGAA 
ACTAGTGACA TAGAAAGCCC AGGTTGTGAC CCTCCCTTAC CAGATCCTGG TCCCAACGAT 
ATGTGCCTAT CAGCAGAGAA GCCCAGGCCG AGTGCCCAGA TCACTTCCAC TCCTGTGGCA 
CGTGGGGCCA CCAGCCTGCA GCAGGAGATC CAAGAGGGCA TCTACTCTGG AAGCTGCTAC 
CACCGGGATG GCTTACAACT GAGTATACAG TTTGAAGTCA AGCGTGTGGA GCTCCAGGGC 
TCTGCGACCC TGTTCTGCTG CTGGCTGGTG AAGGACCTCT TCCATAGCCA CCGGGACTCA 
GCCACACGAA CCCGCCTGTT CCTTGCCAGT CTGCCTAGCT CTACCCACTC CATGCCTGAG 
CTGTCTGGAT CCAGCTTTGG AGAGGTGCTC AGAGCCAAGC CTTGGTTTGA GGAATCCCCA 
ACACCTGCAG AGCTAGAGGG GCTGGCGGCC TGTGAGGGCG AGTACGACTA TAAGTATAAC 
ACCATCAGCC CACTTGGCAG TGGGGCCTTT GGCTTTGTGT GGACTGCAGT GGAGAAGGAA 
TGCAACAAAG AGGTGGTGGT GAAGTTTATT AAGAAGGAGA AGGTTTTAGA GGATTGCTGG 
ATTGAGGATC CCAAACTTGG AAGAGTTACT TTAGAGATTG CAATTCTGTC CAAGGTGGAC 
CATGCCAACA TAATCAAGGT TCTGGATATA TTCGAAAACC AAGAGTTCTT CCAGCTGGTG 
ATGGAAAAGC ACGGCTCTGG CATGGACCTC TTTGCTTTCA TTGACCACCA TCCCTGCTTG 
GATGAGCCCC TGGCGAGTTT CATCTTTCGA CAACTAGTGT CTGCTGTAGG ATACCTGCAC 
TCCCAAGGCA TCATCCATAG AGACATCAAG GATGAGAACA TTGTGATTGC TGAGGACTTC 
ACAATTAAGC TGATAGATTT TGGCTCAGCT GCCTACTTAG AGAGGGGCAA ACTATTTTAT 
ACCTTTTGTG GAACAATCGA ATACTGTGCA CCTGAGGTTC TCATTGGAAA TCCCTACAGA 
GGGCCAGAGC TGGAGATGTG GTCTCTGGGG GTCACCCTGT ACACGCTCAT CTTCGAGGAG 
AATCCCTTCT GTGAGGTGGA GGAGACCATG GAGGCAGTTA TTCATCCCCC ATTCCTGGTT 
TCCCAAGAAC TTATGAGTCT TCTGTCTGGA CTGCTGCAGC CTTGCCCTGA GCAGCGGACC 
ACTTTGGAGA AGCTGATCAG GGACCCCTGG GTGACACAGC CTGTGAACCT TGCTAGCTAT 
ACTTGGGAAG AGGTGTGTAG GACCAACCAG CCAGAAAGTG GCCTGCTGTC AGCTGCAAGT 
CTGGAGATTG GGAGTAGGAG TCCAAGTGAA ATGGCTCAGA GAGAGGGTCT CTGTGGGCCT 
CCTGCTCCCA GGGAGACTCG TGGTGACCAG CACTGCTTGC ATCTTAAGGA CCCCTCTTTG 
CCAGTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pask ( XM_006529665.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529728.1 Mus musculus PAS domain containing serine/threonine kinase (Pask), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu21506
Clone ID Related Accession (Same CDS sequence)	XM_006529665.2 , XM_006529665.4 , XM_006529666.2 , XM_006529666.3 , NM_080850.2 , XM_006529665.3 , XM_006529667.2 , XM_017321037.1
Accession Version	XM_006529665.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	PAS domain-containing serine/threonine-protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529665.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GAGGTCCACC AGTTTTTGCT GAGGACTGGA AATGTTTGTC TGAGAGTCCT 
CCTGTCCAAG AAGGCCCAGC GGCCCAGGCT ACCTTTGAGC CCAGCAAGCC ACTCTCCATA 
GCCCACAAAC ACCTGAGCAG GAAGAATGGG CTTTCTAGAC TCTGCCAGAG CCGAATGGCG 
CTCTCTGAGG ACAGGTGGAG CTCTTACTGT CTTTCATCAT TGGCTGCCCA AAACATCTGC 
ACAAGCAAAC TGCACTGCGC TGCTGCACCT GAGTATGCTG ACCCTACAGC TGGGCCACTG 
GGCAGTACTT CCTGCTGCTC CTTGCTGCGT GGCCTGGCTT CAGGATGCTC TGGGTCCCTG 
CTTTCGACCC CTGTGTGCAA CCCCAACAAG GCTGTCTTTA CCGTGGATGC CAAGACCACA 
GAGATTCTGG TTGCCAACGA CAAAGCCTGC AGCCTCCTAG GGTATAGCAG TCATGACCTG 
ATTGGCCAAA AGCTCGCCCA ATTCTTCCTC AAATCTGATT CCGAGGTGGT GGAAGCCCTC 
AGCGAGGAAC ATGTGGAGGC TGATGGCCAC GCTGCAGTGG TGTTCGGCAC AGTGGTGGAC 
ATCGTCAGCC GAATTGGGGA GAAGATTCCA GTCTCTGTGT GGATAAAGAG ATTGCAGCAA 
GATCGTGGCT TGTGCTGTGT AGTGGTTCTG GAGCCTGTGG AGAGAGTGTC AGCCTGGGTC 
GCCTTCCAGA GTGATGGAAC TATCACATCA TGCGACAGTC TCTTTGCTCA TCTCCATGGG 
TTCACATCTC CAAAGGATGT AGTTGGGCAG TGTGTCATAG ACTTGATTCC ATCCATGCAG 
CTCCCTCCTC CTGGCCAACA TATCCCAAAG AGTCTCAAGA TTCAAAGGTC TGTTGGGAGA 
GCCAGAGACG GCACCACCTT CCCTCTCAGT TTGAAGCTGA AATCCAAGCC CAGTGGAAGG 
GCTGTAGCTG ACAGTGAGGC GGCCTCTGAA CCAGGTTACC AGGCTTCTGT GTGGGTGTTC 
TGCACCATCA GTGGCCTCAT CACTCTGCTG CCAGATGGCA CAATTTATGG AGTCAACCAC 
AGCTTTGCAC TAATGCTGTT TGGTTATGGA AAGACAGAGC TCCTGGGCAA GAATATCACT 
TTCCTGATTC CTGGTTTCTA CCACTACATG GACCTCACAT ATGATAGTTC GGTACAGCTG 
CCAGATCTGG TCAATTGCCT CGACATTGGC AGAAAGAGTG GGCCTGGGGA GATGAACTCT 
GATGCCCAAC ACAACTGGGA ACTGGCCAGT GGGGCCCAGG GTCCAAGAAT TGACGTGGTG 
TTGGCACGGG ACCACATGCC ATCACAAGAT GAGACCCTGA AGCTGGTGGG AGGACAAGTC 
TCCTCTAGGA CTCAGACACG ATTGGAAACT GGATACAAGA TACTTCCTTC TTCTGCATGT 
CAGCCCTCAC TGGGGGTGGA CAGCAACCCA GAGGATGGTG AGCAGTCATT GCTCACAGAC 
CAGCAAAGCA TTCCAAAGAG AAACCTGCCA GCCCATGGTG GTCAGAACCA GCTAGACACC 
TCAGAAATAT CCCTACCAGT CCTTAAAGAA CATTTATTGT CTGAAATTCA GAAAAATATC 
TCAGAAGAAA GCCCACTAAC CCACAGGAAA TGGTTATCCA AGGTGCAACA AAACCCCACA 
AAAGGAAGCC TGCCCATCCA CGAGGAGCAG CTATTGTTTG CAGGCCAGCA TATCCATGTC 
TTGGGAAAGG AGGATCCTTC AGCAGCAGAG AGCTACAGGG AGAGTCTTTT AGAAGAAAGC 
AAGTCCAAAC CAGTGGATGC AAAACTGTTT GCTTCATGTG AAGATTCTGA GCCTCTAGTT 
TCAGTTAAGG ACAGGGGCAG CAGTGTGGAC ACGTGTAATC TGCATCAGGA AGCTCAGTTG 
GAGCTGATGG GTGTCAGCAG TCCTAACCCC TGGGCTGATG CCACCATGCC TGAGCCCCAC 
ACCACAGGGC AGATAGCAGG CGGGAGCCTT ACATATTGCC CACAGTACAG AAGCGAGTGG 
GCCTCACAGC AGCGAGGCCA GGACTCGGCT CCCAGCCCCT CTGGAATGGC GTGTGTCTTG 
CTTGGGACAC CCACTCTGGA TGAACCATGG CCAGGAGTGA GAAATGACAG AGAAGAGCTG 
CAGACTTGCT TGATTAAGGA ACAGTTATCC AAGTCAAGCT GTGAGGGAAA TCTGGGCATC 
TCCCGTGTGG AACTCGTTCC TGAAGAACAT CCTCCTTTCA CCGCCCCCGT GTCTTTCTGT 
GATCTGGGAG GCCGAGATCT ACACGCTAGC CGCTCAGGCA GCTCCTCAGC CTGCTATGCT 
TTGGCCACCG ACCTCCCTGG TGTCCTGGAG GCAGTGGAAG CTCAGGAGGC TGATGTGAAT 
TCTTATTCCT GGAACCTCAA GGAGCTCTTT CTCAAGGACC AGACAGACAG AACTCCATCA 
CATTGTTCCT GTACCACATC TGAACTCAGC GAGGCGCCAT CTCTTTCAGT GGTGGGGTCA 
GATTTAGATG TGGGTATTCT GCATAGGCAG ACTTCAGATA TTTTAGTTGA CCGAGAGATG 
TTGCTCCTTA CTGGGACCTA TTTTGACCTT AGTGAAGGCC AACGGTTCCA GGAGATGGGA 
GCAGGACATG ATCGAGCAGA ACTGTCTAAC ATTTCTTTGG TATCCTCTGA ACACTATGAA 
ACTAGTGACA TAGAAAGCCC AGGTTGTGAC CCTCCCTTAC CAGATCCTGG TCCCAACGAT 
ATGTGCCTAT CAGCAGAGAA GCCCAGGCCG AGTGCCCAGA TCACTTCCAC TCCTGTGGCA 
CGTGGGGCCA CCAGCCTGCA GCAGGAGATC CAAGAGGGCA TCTACTCTGG AAGCTGCTAC 
CACCGGGATG GCTTACAACT GAGTATACAG TTTGAAGTCA AGCGTGTGGA GCTCCAGGGC 
TCTGCGACCC TGTTCTGCTG CTGGCTGGTG AAGGACCTCT TCCATAGCCA CCGGGACTCA 
GCCACACGAA CCCGCCTGTT CCTTGCCAGT CTGCCTAGCT CTACCCACTC CATGCCTGAG 
CTGTCTGGAT CCAGCTTTGG AGAGGTGCTC AGAGCCAAGC CTTGGTTTGA GGAATCCCCA 
ACACCTGCAG AGCTAGAGGG GCTGGCGGCC TGTGAGGGCG AGTACGACTA TAAGTATAAC 
ACCATCAGCC CACTTGGCAG TGGGGCCTTT GGCTTTGTGT GGACTGCAGT GGAGAAGGAA 
TGCAACAAAG AGGTGGTGGT GAAGTTTATT AAGAAGGAGA AGGTTTTAGA GGATTGCTGG 
ATTGAGGATC CCAAACTTGG AAGAGTTACT TTAGAGATTG CAATTCTGTC CAAGGTGGAC 
CATGCCAACA TAATCAAGGT TCTGGATATA TTCGAAAACC AAGAGTTCTT CCAGCTGGTG 
ATGGAAAAGC ACGGCTCTGG CATGGACCTC TTTGCTTTCA TTGACCACCA TCCCTGCTTG 
GATGAGCCCC TGGCGAGTTT CATCTTTCGA CAACTAGTGT CTGCTGTAGG ATACCTGCAC 
TCCCAAGGCA TCATCCATAG AGACATCAAG GATGAGAACA TTGTGATTGC TGAGGACTTC 
ACAATTAAGC TGATAGATTT TGGCTCAGCT GCCTACTTAG AGAGGGGCAA ACTATTTTAT 
ACCTTTTGTG GAACAATCGA ATACTGTGCA CCTGAGGTTC TCATTGGAAA TCCCTACAGA 
GGGCCAGAGC TGGAGATGTG GTCTCTGGGG GTCACCCTGT ACACGCTCAT CTTCGAGGAG 
AATCCCTTCT GTGAGGTGGA GGAGACCATG GAGGCAGTTA TTCATCCCCC ATTCCTGGTT 
TCCCAAGAAC TTATGAGTCT TCTGTCTGGA CTGCTGCAGC CTTGCCCTGA GCAGCGGACC 
ACTTTGGAGA AGCTGATCAG GGACCCCTGG GTGACACAGC CTGTGAACCT TGCTAGCTAT 
ACTTGGGAAG AGGTGTGTAG GACCAACCAG CCAGAAAGTG GCCTGCTGTC AGCTGCAAGT 
CTGGAGATTG GGAGTAGGAG TCCAAGTGAA ATGGCTCAGA GAGAGGGTCT CTGTGGGCCT 
CCTGCTCCCA GGGAGACTCG TGGTGACCAG CACTGCTTGC ATCTTAAGGA CCCCTCTTTG 
CCAGTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529728.1
CDS	183..4334
Translation	MEDRGPPVFAEDWKCLSESPPVQEGPAAQATFEPSKPLSIAHKHLSRKNGLSRLCQSRMALSEDRWSSYCLSSLAAQNICTSKLHCAAAPEYADPTAGPLGSTSCCSLLRGLASGCSGSLLSTPVCNPNKAVFTVDAKTTEILVANDKACSLLGYSSHDLIGQKLAQFFLKSDSEVVEALSEEHVEADGHAAVVFGTVVDIVSRIGEKIPVSVWIKRLQQDRGLCCVVVLEPVERVSAWVAFQSDGTITSCDSLFAHLHGFTSPKDVVGQCVIDLIPSMQLPPPGQHIPKSLKIQRSVGRARDGTTFPLSLKLKSKPSGRAVADSEAASEPGYQASVWVFCTISGLITLLPDGTIYGVNHSFALMLFGYGKTELLGKNITFLIPGFYHYMDLTYDSSVQLPDLVNCLDIGRKSGPGEMNSDAQHNWELASGAQGPRIDVVLARDHMPSQDETLKLVGGQVSSRTQTRLETGYKILPSSACQPSLGVDSNPEDGEQSLLTDQQSIPKRNLPAHGGQNQLDTSEISLPVLKEHLLSEIQKNISEESPLTHRKWLSKVQQNPTKGSLPIHEEQLLFAGQHIHVLGKEDPSAAESYRESLLEESKSKPVDAKLFASCEDSEPLVSVKDRGSSVDTCNLHQEAQLELMGVSSPNPWADATMPEPHTTGQIAGGSLTYCPQYRSEWASQQRGQDSAPSPSGMACVLLGTPTLDEPWPGVRNDREELQTCLIKEQLSKSSCEGNLGISRVELVPEEHPPFTAPVSFCDLGGRDLHASRSGSSSACYALATDLPGVLEAVEAQEADVNSYSWNLKELFLKDQTDRTPSHCSCTTSELSEAPSLSVVGSDLDVGILHRQTSDILVDREMLLLTGTYFDLSEGQRFQEMGAGHDRAELSNISLVSSEHYETSDIESPGCDPPLPDPGPNDMCLSAEKPRPSAQITSTPVARGATSLQQEIQEGIYSGSCYHRDGLQLSIQFEVKRVELQGSATLFCCWLVKDLFHSHRDSATRTRLFLASLPSSTHSMPELSGSSFGEVLRAKPWFEESPTPAELEGLAACEGEYDYKYNTISPLGSGAFGFVWTAVEKECNKEVVVKFIKKEKVLEDCWIEDPKLGRVTLEIAILSKVDHANIIKVLDIFENQEFFQLVMEKHGSGMDLFAFIDHHPCLDEPLASFIFRQLVSAVGYLHSQGIIHRDIKDENIVIAEDFTIKLIDFGSAAYLERGKLFYTFCGTIEYCAPEVLIGNPYRGPELEMWSLGVTLYTLIFEENPFCEVEETMEAVIHPPFLVSQELMSLLSGLLQPCPEQRTTLEKLIRDPWVTQPVNLASYTWEEVCRTNQPESGLLSAASLEIGSRSPSEMAQREGLCGPPAPRETRGDQHCLHLKDPSLPVS

Target ORF information:

RefSeq Version	XM_006529665.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus PAS domain containing serine/threonine kinase (Pask), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529665.3

ORF Insert Sequence:

ATGGAGGACA GAGGTCCACC AGTTTTTGCT GAGGACTGGA AATGTTTGTC TGAGAGTCCT 
CCTGTCCAAG AAGGCCCAGC GGCCCAGGCT ACCTTTGAGC CCAGCAAGCC ACTCTCCATA 
GCCCACAAAC ACCTGAGCAG GAAGAATGGG CTTTCTAGAC TCTGCCAGAG CCGAATGGCG 
CTCTCTGAGG ACAGGTGGAG CTCTTACTGT CTTTCATCAT TGGCTGCCCA AAACATCTGC 
ACAAGCAAAC TGCACTGCGC TGCTGCACCT GAGTATGCTG ACCCTACAGC TGGGCCACTG 
GGCAGTACTT CCTGCTGCTC CTTGCTGCGT GGCCTGGCTT CAGGATGCTC TGGGTCCCTG 
CTTTCGACCC CTGTGTGCAA CCCCAACAAG GCTGTCTTTA CCGTGGATGC CAAGACCACA 
GAGATTCTGG TTGCCAACGA CAAAGCCTGC AGCCTCCTAG GGTATAGCAG TCATGACCTG 
ATTGGCCAAA AGCTCGCCCA ATTCTTCCTC AAATCTGATT CCGAGGTGGT GGAAGCCCTC 
AGCGAGGAAC ATGTGGAGGC TGATGGCCAC GCTGCAGTGG TGTTCGGCAC AGTGGTGGAC 
ATCGTCAGCC GAATTGGGGA GAAGATTCCA GTCTCTGTGT GGATAAAGAG ATTGCAGCAA 
GATCGTGGCT TGTGCTGTGT AGTGGTTCTG GAGCCTGTGG AGAGAGTGTC AGCCTGGGTC 
GCCTTCCAGA GTGATGGAAC TATCACATCA TGCGACAGTC TCTTTGCTCA TCTCCATGGG 
TTCACATCTC CAAAGGATGT AGTTGGGCAG TGTGTCATAG ACTTGATTCC ATCCATGCAG 
CTCCCTCCTC CTGGCCAACA TATCCCAAAG AGTCTCAAGA TTCAAAGGTC TGTTGGGAGA 
GCCAGAGACG GCACCACCTT CCCTCTCAGT TTGAAGCTGA AATCCAAGCC CAGTGGAAGG 
GCTGTAGCTG ACAGTGAGGC GGCCTCTGAA CCAGGTTACC AGGCTTCTGT GTGGGTGTTC 
TGCACCATCA GTGGCCTCAT CACTCTGCTG CCAGATGGCA CAATTTATGG AGTCAACCAC 
AGCTTTGCAC TAATGCTGTT TGGTTATGGA AAGACAGAGC TCCTGGGCAA GAATATCACT 
TTCCTGATTC CTGGTTTCTA CCACTACATG GACCTCACAT ATGATAGTTC GGTACAGCTG 
CCAGATCTGG TCAATTGCCT CGACATTGGC AGAAAGAGTG GGCCTGGGGA GATGAACTCT 
GATGCCCAAC ACAACTGGGA ACTGGCCAGT GGGGCCCAGG GTCCAAGAAT TGACGTGGTG 
TTGGCACGGG ACCACATGCC ATCACAAGAT GAGACCCTGA AGCTGGTGGG AGGACAAGTC 
TCCTCTAGGA CTCAGACACG ATTGGAAACT GGATACAAGA TACTTCCTTC TTCTGCATGT 
CAGCCCTCAC TGGGGGTGGA CAGCAACCCA GAGGATGGTG AGCAGTCATT GCTCACAGAC 
CAGCAAAGCA TTCCAAAGAG AAACCTGCCA GCCCATGGTG GTCAGAACCA GCTAGACACC 
TCAGAAATAT CCCTACCAGT CCTTAAAGAA CATTTATTGT CTGAAATTCA GAAAAATATC 
TCAGAAGAAA GCCCACTAAC CCACAGGAAA TGGTTATCCA AGGTGCAACA AAACCCCACA 
AAAGGAAGCC TGCCCATCCA CGAGGAGCAG CTATTGTTTG CAGGCCAGCA TATCCATGTC 
TTGGGAAAGG AGGATCCTTC AGCAGCAGAG AGCTACAGGG AGAGTCTTTT AGAAGAAAGC 
AAGTCCAAAC CAGTGGATGC AAAACTGTTT GCTTCATGTG AAGATTCTGA GCCTCTAGTT 
TCAGTTAAGG ACAGGGGCAG CAGTGTGGAC ACGTGTAATC TGCATCAGGA AGCTCAGTTG 
GAGCTGATGG GTGTCAGCAG TCCTAACCCC TGGGCTGATG CCACCATGCC TGAGCCCCAC 
ACCACAGGGC AGATAGCAGG CGGGAGCCTT ACATATTGCC CACAGTACAG AAGCGAGTGG 
GCCTCACAGC AGCGAGGCCA GGACTCGGCT CCCAGCCCCT CTGGAATGGC GTGTGTCTTG 
CTTGGGACAC CCACTCTGGA TGAACCATGG CCAGGAGTGA GAAATGACAG AGAAGAGCTG 
CAGACTTGCT TGATTAAGGA ACAGTTATCC AAGTCAAGCT GTGAGGGAAA TCTGGGCATC 
TCCCGTGTGG AACTCGTTCC TGAAGAACAT CCTCCTTTCA CCGCCCCCGT GTCTTTCTGT 
GATCTGGGAG GCCGAGATCT ACACGCTAGC CGCTCAGGCA GCTCCTCAGC CTGCTATGCT 
TTGGCCACCG ACCTCCCTGG TGTCCTGGAG GCAGTGGAAG CTCAGGAGGC TGATGTGAAT 
TCTTATTCCT GGAACCTCAA GGAGCTCTTT CTCAAGGACC AGACAGACAG AACTCCATCA 
CATTGTTCCT GTACCACATC TGAACTCAGC GAGGCGCCAT CTCTTTCAGT GGTGGGGTCA 
GATTTAGATG TGGGTATTCT GCATAGGCAG ACTTCAGATA TTTTAGTTGA CCGAGAGATG 
TTGCTCCTTA CTGGGACCTA TTTTGACCTT AGTGAAGGCC AACGGTTCCA GGAGATGGGA 
GCAGGACATG ATCGAGCAGA ACTGTCTAAC ATTTCTTTGG TATCCTCTGA ACACTATGAA 
ACTAGTGACA TAGAAAGCCC AGGTTGTGAC CCTCCCTTAC CAGATCCTGG TCCCAACGAT 
ATGTGCCTAT CAGCAGAGAA GCCCAGGCCG AGTGCCCAGA TCACTTCCAC TCCTGTGGCA 
CGTGGGGCCA CCAGCCTGCA GCAGGAGATC CAAGAGGGCA TCTACTCTGG AAGCTGCTAC 
CACCGGGATG GCTTACAACT GAGTATACAG TTTGAAGTCA AGCGTGTGGA GCTCCAGGGC 
TCTGCGACCC TGTTCTGCTG CTGGCTGGTG AAGGACCTCT TCCATAGCCA CCGGGACTCA 
GCCACACGAA CCCGCCTGTT CCTTGCCAGT CTGCCTAGCT CTACCCACTC CATGCCTGAG 
CTGTCTGGAT CCAGCTTTGG AGAGGTGCTC AGAGCCAAGC CTTGGTTTGA GGAATCCCCA 
ACACCTGCAG AGCTAGAGGG GCTGGCGGCC TGTGAGGGCG AGTACGACTA TAAGTATAAC 
ACCATCAGCC CACTTGGCAG TGGGGCCTTT GGCTTTGTGT GGACTGCAGT GGAGAAGGAA 
TGCAACAAAG AGGTGGTGGT GAAGTTTATT AAGAAGGAGA AGGTTTTAGA GGATTGCTGG 
ATTGAGGATC CCAAACTTGG AAGAGTTACT TTAGAGATTG CAATTCTGTC CAAGGTGGAC 
CATGCCAACA TAATCAAGGT TCTGGATATA TTCGAAAACC AAGAGTTCTT CCAGCTGGTG 
ATGGAAAAGC ACGGCTCTGG CATGGACCTC TTTGCTTTCA TTGACCACCA TCCCTGCTTG 
GATGAGCCCC TGGCGAGTTT CATCTTTCGA CAACTAGTGT CTGCTGTAGG ATACCTGCAC 
TCCCAAGGCA TCATCCATAG AGACATCAAG GATGAGAACA TTGTGATTGC TGAGGACTTC 
ACAATTAAGC TGATAGATTT TGGCTCAGCT GCCTACTTAG AGAGGGGCAA ACTATTTTAT 
ACCTTTTGTG GAACAATCGA ATACTGTGCA CCTGAGGTTC TCATTGGAAA TCCCTACAGA 
GGGCCAGAGC TGGAGATGTG GTCTCTGGGG GTCACCCTGT ACACGCTCAT CTTCGAGGAG 
AATCCCTTCT GTGAGGTGGA GGAGACCATG GAGGCAGTTA TTCATCCCCC ATTCCTGGTT 
TCCCAAGAAC TTATGAGTCT TCTGTCTGGA CTGCTGCAGC CTTGCCCTGA GCAGCGGACC 
ACTTTGGAGA AGCTGATCAG GGACCCCTGG GTGACACAGC CTGTGAACCT TGCTAGCTAT 
ACTTGGGAAG AGGTGTGTAG GACCAACCAG CCAGAAAGTG GCCTGCTGTC AGCTGCAAGT 
CTGGAGATTG GGAGTAGGAG TCCAAGTGAA ATGGCTCAGA GAGAGGGTCT CTGTGGGCCT 
CCTGCTCCCA GGGAGACTCG TGGTGACCAG CACTGCTTGC ATCTTAAGGA CCCCTCTTTG 
CCAGTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pask ( XM_006529667.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529730.1 Mus musculus PAS domain containing serine/threonine kinase (Pask), transcript variant X3, mRNA.

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CloneID	OMu21506
Clone ID Related Accession (Same CDS sequence)	XM_006529665.2 , XM_006529665.4 , XM_006529666.2 , XM_006529666.3 , NM_080850.2 , XM_006529665.3 , XM_006529667.2 , XM_017321037.1
Accession Version	XM_006529667.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	PAS domain-containing serine/threonine-protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006529667.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GAGGTCCACC AGTTTTTGCT GAGGACTGGA AATGTTTGTC TGAGAGTCCT 
CCTGTCCAAG AAGGCCCAGC GGCCCAGGCT ACCTTTGAGC CCAGCAAGCC ACTCTCCATA 
GCCCACAAAC ACCTGAGCAG GAAGAATGGG CTTTCTAGAC TCTGCCAGAG CCGAATGGCG 
CTCTCTGAGG ACAGGTGGAG CTCTTACTGT CTTTCATCAT TGGCTGCCCA AAACATCTGC 
ACAAGCAAAC TGCACTGCGC TGCTGCACCT GAGTATGCTG ACCCTACAGC TGGGCCACTG 
GGCAGTACTT CCTGCTGCTC CTTGCTGCGT GGCCTGGCTT CAGGATGCTC TGGGTCCCTG 
CTTTCGACCC CTGTGTGCAA CCCCAACAAG GCTGTCTTTA CCGTGGATGC CAAGACCACA 
GAGATTCTGG TTGCCAACGA CAAAGCCTGC AGCCTCCTAG GGTATAGCAG TCATGACCTG 
ATTGGCCAAA AGCTCGCCCA ATTCTTCCTC AAATCTGATT CCGAGGTGGT GGAAGCCCTC 
AGCGAGGAAC ATGTGGAGGC TGATGGCCAC GCTGCAGTGG TGTTCGGCAC AGTGGTGGAC 
ATCGTCAGCC GAATTGGGGA GAAGATTCCA GTCTCTGTGT GGATAAAGAG ATTGCAGCAA 
GATCGTGGCT TGTGCTGTGT AGTGGTTCTG GAGCCTGTGG AGAGAGTGTC AGCCTGGGTC 
GCCTTCCAGA GTGATGGAAC TATCACATCA TGCGACAGTC TCTTTGCTCA TCTCCATGGG 
TTCACATCTC CAAAGGATGT AGTTGGGCAG TGTGTCATAG ACTTGATTCC ATCCATGCAG 
CTCCCTCCTC CTGGCCAACA TATCCCAAAG AGTCTCAAGA TTCAAAGGTC TGTTGGGAGA 
GCCAGAGACG GCACCACCTT CCCTCTCAGT TTGAAGCTGA AATCCAAGCC CAGTGGAAGG 
GCTGTAGCTG ACAGTGAGGC GGCCTCTGAA CCAGGTTACC AGGCTTCTGT GTGGGTGTTC 
TGCACCATCA GTGGCCTCAT CACTCTGCTG CCAGATGGCA CAATTTATGG AGTCAACCAC 
AGCTTTGCAC TAATGCTGTT TGGTTATGGA AAGACAGAGC TCCTGGGCAA GAATATCACT 
TTCCTGATTC CTGGTTTCTA CCACTACATG GACCTCACAT ATGATAGTTC GGTACAGCTG 
CCAGATCTGG TCAATTGCCT CGACATTGGC AGAAAGAGTG GGCCTGGGGA GATGAACTCT 
GATGCCCAAC ACAACTGGGA ACTGGCCAGT GGGGCCCAGG GTCCAAGAAT TGACGTGGTG 
TTGGCACGGG ACCACATGCC ATCACAAGAT GAGACCCTGA AGCTGGTGGG AGGACAAGTC 
TCCTCTAGGA CTCAGACACG ATTGGAAACT GGATACAAGA TACTTCCTTC TTCTGCATGT 
CAGCCCTCAC TGGGGGTGGA CAGCAACCCA GAGGATGGTG AGCAGTCATT GCTCACAGAC 
CAGCAAAGCA TTCCAAAGAG AAACCTGCCA GCCCATGGTG GTCAGAACCA GCTAGACACC 
TCAGAAATAT CCCTACCAGT CCTTAAAGAA CATTTATTGT CTGAAATTCA GAAAAATATC 
TCAGAAGAAA GCCCACTAAC CCACAGGAAA TGGTTATCCA AGGTGCAACA AAACCCCACA 
AAAGGAAGCC TGCCCATCCA CGAGGAGCAG CTATTGTTTG CAGGCCAGCA TATCCATGTC 
TTGGGAAAGG AGGATCCTTC AGCAGCAGAG AGCTACAGGG AGAGTCTTTT AGAAGAAAGC 
AAGTCCAAAC CAGTGGATGC AAAACTGTTT GCTTCATGTG AAGATTCTGA GCCTCTAGTT 
TCAGTTAAGG ACAGGGGCAG CAGTGTGGAC ACGTGTAATC TGCATCAGGA AGCTCAGTTG 
GAGCTGATGG GTGTCAGCAG TCCTAACCCC TGGGCTGATG CCACCATGCC TGAGCCCCAC 
ACCACAGGGC AGATAGCAGG CGGGAGCCTT ACATATTGCC CACAGTACAG AAGCGAGTGG 
GCCTCACAGC AGCGAGGCCA GGACTCGGCT CCCAGCCCCT CTGGAATGGC GTGTGTCTTG 
CTTGGGACAC CCACTCTGGA TGAACCATGG CCAGGAGTGA GAAATGACAG AGAAGAGCTG 
CAGACTTGCT TGATTAAGGA ACAGTTATCC AAGTCAAGCT GTGAGGGAAA TCTGGGCATC 
TCCCGTGTGG AACTCGTTCC TGAAGAACAT CCTCCTTTCA CCGCCCCCGT GTCTTTCTGT 
GATCTGGGAG GCCGAGATCT ACACGCTAGC CGCTCAGGCA GCTCCTCAGC CTGCTATGCT 
TTGGCCACCG ACCTCCCTGG TGTCCTGGAG GCAGTGGAAG CTCAGGAGGC TGATGTGAAT 
TCTTATTCCT GGAACCTCAA GGAGCTCTTT CTCAAGGACC AGACAGACAG AACTCCATCA 
CATTGTTCCT GTACCACATC TGAACTCAGC GAGGCGCCAT CTCTTTCAGT GGTGGGGTCA 
GATTTAGATG TGGGTATTCT GCATAGGCAG ACTTCAGATA TTTTAGTTGA CCGAGAGATG 
TTGCTCCTTA CTGGGACCTA TTTTGACCTT AGTGAAGGCC AACGGTTCCA GGAGATGGGA 
GCAGGACATG ATCGAGCAGA ACTGTCTAAC ATTTCTTTGG TATCCTCTGA ACACTATGAA 
ACTAGTGACA TAGAAAGCCC AGGTTGTGAC CCTCCCTTAC CAGATCCTGG TCCCAACGAT 
ATGTGCCTAT CAGCAGAGAA GCCCAGGCCG AGTGCCCAGA TCACTTCCAC TCCTGTGGCA 
CGTGGGGCCA CCAGCCTGCA GCAGGAGATC CAAGAGGGCA TCTACTCTGG AAGCTGCTAC 
CACCGGGATG GCTTACAACT GAGTATACAG TTTGAAGTCA AGCGTGTGGA GCTCCAGGGC 
TCTGCGACCC TGTTCTGCTG CTGGCTGGTG AAGGACCTCT TCCATAGCCA CCGGGACTCA 
GCCACACGAA CCCGCCTGTT CCTTGCCAGT CTGCCTAGCT CTACCCACTC CATGCCTGAG 
CTGTCTGGAT CCAGCTTTGG AGAGGTGCTC AGAGCCAAGC CTTGGTTTGA GGAATCCCCA 
ACACCTGCAG AGCTAGAGGG GCTGGCGGCC TGTGAGGGCG AGTACGACTA TAAGTATAAC 
ACCATCAGCC CACTTGGCAG TGGGGCCTTT GGCTTTGTGT GGACTGCAGT GGAGAAGGAA 
TGCAACAAAG AGGTGGTGGT GAAGTTTATT AAGAAGGAGA AGGTTTTAGA GGATTGCTGG 
ATTGAGGATC CCAAACTTGG AAGAGTTACT TTAGAGATTG CAATTCTGTC CAAGGTGGAC 
CATGCCAACA TAATCAAGGT TCTGGATATA TTCGAAAACC AAGAGTTCTT CCAGCTGGTG 
ATGGAAAAGC ACGGCTCTGG CATGGACCTC TTTGCTTTCA TTGACCACCA TCCCTGCTTG 
GATGAGCCCC TGGCGAGTTT CATCTTTCGA CAACTAGTGT CTGCTGTAGG ATACCTGCAC 
TCCCAAGGCA TCATCCATAG AGACATCAAG GATGAGAACA TTGTGATTGC TGAGGACTTC 
ACAATTAAGC TGATAGATTT TGGCTCAGCT GCCTACTTAG AGAGGGGCAA ACTATTTTAT 
ACCTTTTGTG GAACAATCGA ATACTGTGCA CCTGAGGTTC TCATTGGAAA TCCCTACAGA 
GGGCCAGAGC TGGAGATGTG GTCTCTGGGG GTCACCCTGT ACACGCTCAT CTTCGAGGAG 
AATCCCTTCT GTGAGGTGGA GGAGACCATG GAGGCAGTTA TTCATCCCCC ATTCCTGGTT 
TCCCAAGAAC TTATGAGTCT TCTGTCTGGA CTGCTGCAGC CTTGCCCTGA GCAGCGGACC 
ACTTTGGAGA AGCTGATCAG GGACCCCTGG GTGACACAGC CTGTGAACCT TGCTAGCTAT 
ACTTGGGAAG AGGTGTGTAG GACCAACCAG CCAGAAAGTG GCCTGCTGTC AGCTGCAAGT 
CTGGAGATTG GGAGTAGGAG TCCAAGTGAA ATGGCTCAGA GAGAGGGTCT CTGTGGGCCT 
CCTGCTCCCA GGGAGACTCG TGGTGACCAG CACTGCTTGC ATCTTAAGGA CCCCTCTTTG 
CCAGTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529730.1
CDS	128..4279
Translation	MEDRGPPVFAEDWKCLSESPPVQEGPAAQATFEPSKPLSIAHKHLSRKNGLSRLCQSRMALSEDRWSSYCLSSLAAQNICTSKLHCAAAPEYADPTAGPLGSTSCCSLLRGLASGCSGSLLSTPVCNPNKAVFTVDAKTTEILVANDKACSLLGYSSHDLIGQKLAQFFLKSDSEVVEALSEEHVEADGHAAVVFGTVVDIVSRIGEKIPVSVWIKRLQQDRGLCCVVVLEPVERVSAWVAFQSDGTITSCDSLFAHLHGFTSPKDVVGQCVIDLIPSMQLPPPGQHIPKSLKIQRSVGRARDGTTFPLSLKLKSKPSGRAVADSEAASEPGYQASVWVFCTISGLITLLPDGTIYGVNHSFALMLFGYGKTELLGKNITFLIPGFYHYMDLTYDSSVQLPDLVNCLDIGRKSGPGEMNSDAQHNWELASGAQGPRIDVVLARDHMPSQDETLKLVGGQVSSRTQTRLETGYKILPSSACQPSLGVDSNPEDGEQSLLTDQQSIPKRNLPAHGGQNQLDTSEISLPVLKEHLLSEIQKNISEESPLTHRKWLSKVQQNPTKGSLPIHEEQLLFAGQHIHVLGKEDPSAAESYRESLLEESKSKPVDAKLFASCEDSEPLVSVKDRGSSVDTCNLHQEAQLELMGVSSPNPWADATMPEPHTTGQIAGGSLTYCPQYRSEWASQQRGQDSAPSPSGMACVLLGTPTLDEPWPGVRNDREELQTCLIKEQLSKSSCEGNLGISRVELVPEEHPPFTAPVSFCDLGGRDLHASRSGSSSACYALATDLPGVLEAVEAQEADVNSYSWNLKELFLKDQTDRTPSHCSCTTSELSEAPSLSVVGSDLDVGILHRQTSDILVDREMLLLTGTYFDLSEGQRFQEMGAGHDRAELSNISLVSSEHYETSDIESPGCDPPLPDPGPNDMCLSAEKPRPSAQITSTPVARGATSLQQEIQEGIYSGSCYHRDGLQLSIQFEVKRVELQGSATLFCCWLVKDLFHSHRDSATRTRLFLASLPSSTHSMPELSGSSFGEVLRAKPWFEESPTPAELEGLAACEGEYDYKYNTISPLGSGAFGFVWTAVEKECNKEVVVKFIKKEKVLEDCWIEDPKLGRVTLEIAILSKVDHANIIKVLDIFENQEFFQLVMEKHGSGMDLFAFIDHHPCLDEPLASFIFRQLVSAVGYLHSQGIIHRDIKDENIVIAEDFTIKLIDFGSAAYLERGKLFYTFCGTIEYCAPEVLIGNPYRGPELEMWSLGVTLYTLIFEENPFCEVEETMEAVIHPPFLVSQELMSLLSGLLQPCPEQRTTLEKLIRDPWVTQPVNLASYTWEEVCRTNQPESGLLSAASLEIGSRSPSEMAQREGLCGPPAPRETRGDQHCLHLKDPSLPVS

Target ORF information:

RefSeq Version	XM_006529667.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus PAS domain containing serine/threonine kinase (Pask), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529667.2

ORF Insert Sequence:

ATGGAGGACA GAGGTCCACC AGTTTTTGCT GAGGACTGGA AATGTTTGTC TGAGAGTCCT 
CCTGTCCAAG AAGGCCCAGC GGCCCAGGCT ACCTTTGAGC CCAGCAAGCC ACTCTCCATA 
GCCCACAAAC ACCTGAGCAG GAAGAATGGG CTTTCTAGAC TCTGCCAGAG CCGAATGGCG 
CTCTCTGAGG ACAGGTGGAG CTCTTACTGT CTTTCATCAT TGGCTGCCCA AAACATCTGC 
ACAAGCAAAC TGCACTGCGC TGCTGCACCT GAGTATGCTG ACCCTACAGC TGGGCCACTG 
GGCAGTACTT CCTGCTGCTC CTTGCTGCGT GGCCTGGCTT CAGGATGCTC TGGGTCCCTG 
CTTTCGACCC CTGTGTGCAA CCCCAACAAG GCTGTCTTTA CCGTGGATGC CAAGACCACA 
GAGATTCTGG TTGCCAACGA CAAAGCCTGC AGCCTCCTAG GGTATAGCAG TCATGACCTG 
ATTGGCCAAA AGCTCGCCCA ATTCTTCCTC AAATCTGATT CCGAGGTGGT GGAAGCCCTC 
AGCGAGGAAC ATGTGGAGGC TGATGGCCAC GCTGCAGTGG TGTTCGGCAC AGTGGTGGAC 
ATCGTCAGCC GAATTGGGGA GAAGATTCCA GTCTCTGTGT GGATAAAGAG ATTGCAGCAA 
GATCGTGGCT TGTGCTGTGT AGTGGTTCTG GAGCCTGTGG AGAGAGTGTC AGCCTGGGTC 
GCCTTCCAGA GTGATGGAAC TATCACATCA TGCGACAGTC TCTTTGCTCA TCTCCATGGG 
TTCACATCTC CAAAGGATGT AGTTGGGCAG TGTGTCATAG ACTTGATTCC ATCCATGCAG 
CTCCCTCCTC CTGGCCAACA TATCCCAAAG AGTCTCAAGA TTCAAAGGTC TGTTGGGAGA 
GCCAGAGACG GCACCACCTT CCCTCTCAGT TTGAAGCTGA AATCCAAGCC CAGTGGAAGG 
GCTGTAGCTG ACAGTGAGGC GGCCTCTGAA CCAGGTTACC AGGCTTCTGT GTGGGTGTTC 
TGCACCATCA GTGGCCTCAT CACTCTGCTG CCAGATGGCA CAATTTATGG AGTCAACCAC 
AGCTTTGCAC TAATGCTGTT TGGTTATGGA AAGACAGAGC TCCTGGGCAA GAATATCACT 
TTCCTGATTC CTGGTTTCTA CCACTACATG GACCTCACAT ATGATAGTTC GGTACAGCTG 
CCAGATCTGG TCAATTGCCT CGACATTGGC AGAAAGAGTG GGCCTGGGGA GATGAACTCT 
GATGCCCAAC ACAACTGGGA ACTGGCCAGT GGGGCCCAGG GTCCAAGAAT TGACGTGGTG 
TTGGCACGGG ACCACATGCC ATCACAAGAT GAGACCCTGA AGCTGGTGGG AGGACAAGTC 
TCCTCTAGGA CTCAGACACG ATTGGAAACT GGATACAAGA TACTTCCTTC TTCTGCATGT 
CAGCCCTCAC TGGGGGTGGA CAGCAACCCA GAGGATGGTG AGCAGTCATT GCTCACAGAC 
CAGCAAAGCA TTCCAAAGAG AAACCTGCCA GCCCATGGTG GTCAGAACCA GCTAGACACC 
TCAGAAATAT CCCTACCAGT CCTTAAAGAA CATTTATTGT CTGAAATTCA GAAAAATATC 
TCAGAAGAAA GCCCACTAAC CCACAGGAAA TGGTTATCCA AGGTGCAACA AAACCCCACA 
AAAGGAAGCC TGCCCATCCA CGAGGAGCAG CTATTGTTTG CAGGCCAGCA TATCCATGTC 
TTGGGAAAGG AGGATCCTTC AGCAGCAGAG AGCTACAGGG AGAGTCTTTT AGAAGAAAGC 
AAGTCCAAAC CAGTGGATGC AAAACTGTTT GCTTCATGTG AAGATTCTGA GCCTCTAGTT 
TCAGTTAAGG ACAGGGGCAG CAGTGTGGAC ACGTGTAATC TGCATCAGGA AGCTCAGTTG 
GAGCTGATGG GTGTCAGCAG TCCTAACCCC TGGGCTGATG CCACCATGCC TGAGCCCCAC 
ACCACAGGGC AGATAGCAGG CGGGAGCCTT ACATATTGCC CACAGTACAG AAGCGAGTGG 
GCCTCACAGC AGCGAGGCCA GGACTCGGCT CCCAGCCCCT CTGGAATGGC GTGTGTCTTG 
CTTGGGACAC CCACTCTGGA TGAACCATGG CCAGGAGTGA GAAATGACAG AGAAGAGCTG 
CAGACTTGCT TGATTAAGGA ACAGTTATCC AAGTCAAGCT GTGAGGGAAA TCTGGGCATC 
TCCCGTGTGG AACTCGTTCC TGAAGAACAT CCTCCTTTCA CCGCCCCCGT GTCTTTCTGT 
GATCTGGGAG GCCGAGATCT ACACGCTAGC CGCTCAGGCA GCTCCTCAGC CTGCTATGCT 
TTGGCCACCG ACCTCCCTGG TGTCCTGGAG GCAGTGGAAG CTCAGGAGGC TGATGTGAAT 
TCTTATTCCT GGAACCTCAA GGAGCTCTTT CTCAAGGACC AGACAGACAG AACTCCATCA 
CATTGTTCCT GTACCACATC TGAACTCAGC GAGGCGCCAT CTCTTTCAGT GGTGGGGTCA 
GATTTAGATG TGGGTATTCT GCATAGGCAG ACTTCAGATA TTTTAGTTGA CCGAGAGATG 
TTGCTCCTTA CTGGGACCTA TTTTGACCTT AGTGAAGGCC AACGGTTCCA GGAGATGGGA 
GCAGGACATG ATCGAGCAGA ACTGTCTAAC ATTTCTTTGG TATCCTCTGA ACACTATGAA 
ACTAGTGACA TAGAAAGCCC AGGTTGTGAC CCTCCCTTAC CAGATCCTGG TCCCAACGAT 
ATGTGCCTAT CAGCAGAGAA GCCCAGGCCG AGTGCCCAGA TCACTTCCAC TCCTGTGGCA 
CGTGGGGCCA CCAGCCTGCA GCAGGAGATC CAAGAGGGCA TCTACTCTGG AAGCTGCTAC 
CACCGGGATG GCTTACAACT GAGTATACAG TTTGAAGTCA AGCGTGTGGA GCTCCAGGGC 
TCTGCGACCC TGTTCTGCTG CTGGCTGGTG AAGGACCTCT TCCATAGCCA CCGGGACTCA 
GCCACACGAA CCCGCCTGTT CCTTGCCAGT CTGCCTAGCT CTACCCACTC CATGCCTGAG 
CTGTCTGGAT CCAGCTTTGG AGAGGTGCTC AGAGCCAAGC CTTGGTTTGA GGAATCCCCA 
ACACCTGCAG AGCTAGAGGG GCTGGCGGCC TGTGAGGGCG AGTACGACTA TAAGTATAAC 
ACCATCAGCC CACTTGGCAG TGGGGCCTTT GGCTTTGTGT GGACTGCAGT GGAGAAGGAA 
TGCAACAAAG AGGTGGTGGT GAAGTTTATT AAGAAGGAGA AGGTTTTAGA GGATTGCTGG 
ATTGAGGATC CCAAACTTGG AAGAGTTACT TTAGAGATTG CAATTCTGTC CAAGGTGGAC 
CATGCCAACA TAATCAAGGT TCTGGATATA TTCGAAAACC AAGAGTTCTT CCAGCTGGTG 
ATGGAAAAGC ACGGCTCTGG CATGGACCTC TTTGCTTTCA TTGACCACCA TCCCTGCTTG 
GATGAGCCCC TGGCGAGTTT CATCTTTCGA CAACTAGTGT CTGCTGTAGG ATACCTGCAC 
TCCCAAGGCA TCATCCATAG AGACATCAAG GATGAGAACA TTGTGATTGC TGAGGACTTC 
ACAATTAAGC TGATAGATTT TGGCTCAGCT GCCTACTTAG AGAGGGGCAA ACTATTTTAT 
ACCTTTTGTG GAACAATCGA ATACTGTGCA CCTGAGGTTC TCATTGGAAA TCCCTACAGA 
GGGCCAGAGC TGGAGATGTG GTCTCTGGGG GTCACCCTGT ACACGCTCAT CTTCGAGGAG 
AATCCCTTCT GTGAGGTGGA GGAGACCATG GAGGCAGTTA TTCATCCCCC ATTCCTGGTT 
TCCCAAGAAC TTATGAGTCT TCTGTCTGGA CTGCTGCAGC CTTGCCCTGA GCAGCGGACC 
ACTTTGGAGA AGCTGATCAG GGACCCCTGG GTGACACAGC CTGTGAACCT TGCTAGCTAT 
ACTTGGGAAG AGGTGTGTAG GACCAACCAG CCAGAAAGTG GCCTGCTGTC AGCTGCAAGT 
CTGGAGATTG GGAGTAGGAG TCCAAGTGAA ATGGCTCAGA GAGAGGGTCT CTGTGGGCCT 
CCTGCTCCCA GGGAGACTCG TGGTGACCAG CACTGCTTGC ATCTTAAGGA CCCCTCTTTG 
CCAGTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pask ( XM_017321037.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176526.1 Mus musculus PAS domain containing serine/threonine kinase (Pask), transcript variant X4, mRNA.

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CloneID	OMu21506
Clone ID Related Accession (Same CDS sequence)	XM_006529665.2 , XM_006529665.4 , XM_006529666.2 , XM_006529666.3 , NM_080850.2 , XM_006529665.3 , XM_006529667.2 , XM_017321037.1
Accession Version	XM_017321037.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	PAS domain-containing serine/threonine-protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GAGGTCCACC AGTTTTTGCT GAGGACTGGA AATGTTTGTC TGAGAGTCCT 
CCTGTCCAAG AAGGCCCAGC GGCCCAGGCT ACCTTTGAGC CCAGCAAGCC ACTCTCCATA 
GCCCACAAAC ACCTGAGCAG GAAGAATGGG CTTTCTAGAC TCTGCCAGAG CCGAATGGCG 
CTCTCTGAGG ACAGGTGGAG CTCTTACTGT CTTTCATCAT TGGCTGCCCA AAACATCTGC 
ACAAGCAAAC TGCACTGCGC TGCTGCACCT GAGTATGCTG ACCCTACAGC TGGGCCACTG 
GGCAGTACTT CCTGCTGCTC CTTGCTGCGT GGCCTGGCTT CAGGATGCTC TGGGTCCCTG 
CTTTCGACCC CTGTGTGCAA CCCCAACAAG GCTGTCTTTA CCGTGGATGC CAAGACCACA 
GAGATTCTGG TTGCCAACGA CAAAGCCTGC AGCCTCCTAG GGTATAGCAG TCATGACCTG 
ATTGGCCAAA AGCTCGCCCA ATTCTTCCTC AAATCTGATT CCGAGGTGGT GGAAGCCCTC 
AGCGAGGAAC ATGTGGAGGC TGATGGCCAC GCTGCAGTGG TGTTCGGCAC AGTGGTGGAC 
ATCGTCAGCC GAATTGGGGA GAAGATTCCA GTCTCTGTGT GGATAAAGAG ATTGCAGCAA 
GATCGTGGCT TGTGCTGTGT AGTGGTTCTG GAGCCTGTGG AGAGAGTGTC AGCCTGGGTC 
GCCTTCCAGA GTGATGGAAC TATCACATCA TGCGACAGTC TCTTTGCTCA TCTCCATGGG 
TTCACATCTC CAAAGGATGT AGTTGGGCAG TGTGTCATAG ACTTGATTCC ATCCATGCAG 
CTCCCTCCTC CTGGCCAACA TATCCCAAAG AGTCTCAAGA TTCAAAGGTC TGTTGGGAGA 
GCCAGAGACG GCACCACCTT CCCTCTCAGT TTGAAGCTGA AATCCAAGCC CAGTGGAAGG 
GCTGTAGCTG ACAGTGAGGC GGCCTCTGAA CCAGGTTACC AGGCTTCTGT GTGGGTGTTC 
TGCACCATCA GTGGCCTCAT CACTCTGCTG CCAGATGGCA CAATTTATGG AGTCAACCAC 
AGCTTTGCAC TAATGCTGTT TGGTTATGGA AAGACAGAGC TCCTGGGCAA GAATATCACT 
TTCCTGATTC CTGGTTTCTA CCACTACATG GACCTCACAT ATGATAGTTC GGTACAGCTG 
CCAGATCTGG TCAATTGCCT CGACATTGGC AGAAAGAGTG GGCCTGGGGA GATGAACTCT 
GATGCCCAAC ACAACTGGGA ACTGGCCAGT GGGGCCCAGG GTCCAAGAAT TGACGTGGTG 
TTGGCACGGG ACCACATGCC ATCACAAGAT GAGACCCTGA AGCTGGTGGG AGGACAAGTC 
TCCTCTAGGA CTCAGACACG ATTGGAAACT GGATACAAGA TACTTCCTTC TTCTGCATGT 
CAGCCCTCAC TGGGGGTGGA CAGCAACCCA GAGGATGGTG AGCAGTCATT GCTCACAGAC 
CAGCAAAGCA TTCCAAAGAG AAACCTGCCA GCCCATGGTG GTCAGAACCA GCTAGACACC 
TCAGAAATAT CCCTACCAGT CCTTAAAGAA CATTTATTGT CTGAAATTCA GAAAAATATC 
TCAGAAGAAA GCCCACTAAC CCACAGGAAA TGGTTATCCA AGGTGCAACA AAACCCCACA 
AAAGGAAGCC TGCCCATCCA CGAGGAGCAG CTATTGTTTG CAGGCCAGCA TATCCATGTC 
TTGGGAAAGG AGGATCCTTC AGCAGCAGAG AGCTACAGGG AGAGTCTTTT AGAAGAAAGC 
AAGTCCAAAC CAGTGGATGC AAAACTGTTT GCTTCATGTG AAGATTCTGA GCCTCTAGTT 
TCAGTTAAGG ACAGGGGCAG CAGTGTGGAC ACGTGTAATC TGCATCAGGA AGCTCAGTTG 
GAGCTGATGG GTGTCAGCAG TCCTAACCCC TGGGCTGATG CCACCATGCC TGAGCCCCAC 
ACCACAGGGC AGATAGCAGG CGGGAGCCTT ACATATTGCC CACAGTACAG AAGCGAGTGG 
GCCTCACAGC AGCGAGGCCA GGACTCGGCT CCCAGCCCCT CTGGAATGGC GTGTGTCTTG 
CTTGGGACAC CCACTCTGGA TGAACCATGG CCAGGAGTGA GAAATGACAG AGAAGAGCTG 
CAGACTTGCT TGATTAAGGA ACAGTTATCC AAGTCAAGCT GTGAGGGAAA TCTGGGCATC 
TCCCGTGTGG AACTCGTTCC TGAAGAACAT CCTCCTTTCA CCGCCCCCGT GTCTTTCTGT 
GATCTGGGAG GCCGAGATCT ACACGCTAGC CGCTCAGGCA GCTCCTCAGC CTGCTATGCT 
TTGGCCACCG ACCTCCCTGG TGTCCTGGAG GCAGTGGAAG CTCAGGAGGC TGATGTGAAT 
TCTTATTCCT GGAACCTCAA GGAGCTCTTT CTCAAGGACC AGACAGACAG AACTCCATCA 
CATTGTTCCT GTACCACATC TGAACTCAGC GAGGCGCCAT CTCTTTCAGT GGTGGGGTCA 
GATTTAGATG TGGGTATTCT GCATAGGCAG ACTTCAGATA TTTTAGTTGA CCGAGAGATG 
TTGCTCCTTA CTGGGACCTA TTTTGACCTT AGTGAAGGCC AACGGTTCCA GGAGATGGGA 
GCAGGACATG ATCGAGCAGA ACTGTCTAAC ATTTCTTTGG TATCCTCTGA ACACTATGAA 
ACTAGTGACA TAGAAAGCCC AGGTTGTGAC CCTCCCTTAC CAGATCCTGG TCCCAACGAT 
ATGTGCCTAT CAGCAGAGAA GCCCAGGCCG AGTGCCCAGA TCACTTCCAC TCCTGTGGCA 
CGTGGGGCCA CCAGCCTGCA GCAGGAGATC CAAGAGGGCA TCTACTCTGG AAGCTGCTAC 
CACCGGGATG GCTTACAACT GAGTATACAG TTTGAAGTCA AGCGTGTGGA GCTCCAGGGC 
TCTGCGACCC TGTTCTGCTG CTGGCTGGTG AAGGACCTCT TCCATAGCCA CCGGGACTCA 
GCCACACGAA CCCGCCTGTT CCTTGCCAGT CTGCCTAGCT CTACCCACTC CATGCCTGAG 
CTGTCTGGAT CCAGCTTTGG AGAGGTGCTC AGAGCCAAGC CTTGGTTTGA GGAATCCCCA 
ACACCTGCAG AGCTAGAGGG GCTGGCGGCC TGTGAGGGCG AGTACGACTA TAAGTATAAC 
ACCATCAGCC CACTTGGCAG TGGGGCCTTT GGCTTTGTGT GGACTGCAGT GGAGAAGGAA 
TGCAACAAAG AGGTGGTGGT GAAGTTTATT AAGAAGGAGA AGGTTTTAGA GGATTGCTGG 
ATTGAGGATC CCAAACTTGG AAGAGTTACT TTAGAGATTG CAATTCTGTC CAAGGTGGAC 
CATGCCAACA TAATCAAGGT TCTGGATATA TTCGAAAACC AAGAGTTCTT CCAGCTGGTG 
ATGGAAAAGC ACGGCTCTGG CATGGACCTC TTTGCTTTCA TTGACCACCA TCCCTGCTTG 
GATGAGCCCC TGGCGAGTTT CATCTTTCGA CAACTAGTGT CTGCTGTAGG ATACCTGCAC 
TCCCAAGGCA TCATCCATAG AGACATCAAG GATGAGAACA TTGTGATTGC TGAGGACTTC 
ACAATTAAGC TGATAGATTT TGGCTCAGCT GCCTACTTAG AGAGGGGCAA ACTATTTTAT 
ACCTTTTGTG GAACAATCGA ATACTGTGCA CCTGAGGTTC TCATTGGAAA TCCCTACAGA 
GGGCCAGAGC TGGAGATGTG GTCTCTGGGG GTCACCCTGT ACACGCTCAT CTTCGAGGAG 
AATCCCTTCT GTGAGGTGGA GGAGACCATG GAGGCAGTTA TTCATCCCCC ATTCCTGGTT 
TCCCAAGAAC TTATGAGTCT TCTGTCTGGA CTGCTGCAGC CTTGCCCTGA GCAGCGGACC 
ACTTTGGAGA AGCTGATCAG GGACCCCTGG GTGACACAGC CTGTGAACCT TGCTAGCTAT 
ACTTGGGAAG AGGTGTGTAG GACCAACCAG CCAGAAAGTG GCCTGCTGTC AGCTGCAAGT 
CTGGAGATTG GGAGTAGGAG TCCAAGTGAA ATGGCTCAGA GAGAGGGTCT CTGTGGGCCT 
CCTGCTCCCA GGGAGACTCG TGGTGACCAG CACTGCTTGC ATCTTAAGGA CCCCTCTTTG 
CCAGTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176526.1
CDS	135..4286
Translation	MEDRGPPVFAEDWKCLSESPPVQEGPAAQATFEPSKPLSIAHKHLSRKNGLSRLCQSRMALSEDRWSSYCLSSLAAQNICTSKLHCAAAPEYADPTAGPLGSTSCCSLLRGLASGCSGSLLSTPVCNPNKAVFTVDAKTTEILVANDKACSLLGYSSHDLIGQKLAQFFLKSDSEVVEALSEEHVEADGHAAVVFGTVVDIVSRIGEKIPVSVWIKRLQQDRGLCCVVVLEPVERVSAWVAFQSDGTITSCDSLFAHLHGFTSPKDVVGQCVIDLIPSMQLPPPGQHIPKSLKIQRSVGRARDGTTFPLSLKLKSKPSGRAVADSEAASEPGYQASVWVFCTISGLITLLPDGTIYGVNHSFALMLFGYGKTELLGKNITFLIPGFYHYMDLTYDSSVQLPDLVNCLDIGRKSGPGEMNSDAQHNWELASGAQGPRIDVVLARDHMPSQDETLKLVGGQVSSRTQTRLETGYKILPSSACQPSLGVDSNPEDGEQSLLTDQQSIPKRNLPAHGGQNQLDTSEISLPVLKEHLLSEIQKNISEESPLTHRKWLSKVQQNPTKGSLPIHEEQLLFAGQHIHVLGKEDPSAAESYRESLLEESKSKPVDAKLFASCEDSEPLVSVKDRGSSVDTCNLHQEAQLELMGVSSPNPWADATMPEPHTTGQIAGGSLTYCPQYRSEWASQQRGQDSAPSPSGMACVLLGTPTLDEPWPGVRNDREELQTCLIKEQLSKSSCEGNLGISRVELVPEEHPPFTAPVSFCDLGGRDLHASRSGSSSACYALATDLPGVLEAVEAQEADVNSYSWNLKELFLKDQTDRTPSHCSCTTSELSEAPSLSVVGSDLDVGILHRQTSDILVDREMLLLTGTYFDLSEGQRFQEMGAGHDRAELSNISLVSSEHYETSDIESPGCDPPLPDPGPNDMCLSAEKPRPSAQITSTPVARGATSLQQEIQEGIYSGSCYHRDGLQLSIQFEVKRVELQGSATLFCCWLVKDLFHSHRDSATRTRLFLASLPSSTHSMPELSGSSFGEVLRAKPWFEESPTPAELEGLAACEGEYDYKYNTISPLGSGAFGFVWTAVEKECNKEVVVKFIKKEKVLEDCWIEDPKLGRVTLEIAILSKVDHANIIKVLDIFENQEFFQLVMEKHGSGMDLFAFIDHHPCLDEPLASFIFRQLVSAVGYLHSQGIIHRDIKDENIVIAEDFTIKLIDFGSAAYLERGKLFYTFCGTIEYCAPEVLIGNPYRGPELEMWSLGVTLYTLIFEENPFCEVEETMEAVIHPPFLVSQELMSLLSGLLQPCPEQRTTLEKLIRDPWVTQPVNLASYTWEEVCRTNQPESGLLSAASLEIGSRSPSEMAQREGLCGPPAPRETRGDQHCLHLKDPSLPVS

Target ORF information:

RefSeq Version	XM_017321037.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus PAS domain containing serine/threonine kinase (Pask), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321037.1

ORF Insert Sequence:

ATGGAGGACA GAGGTCCACC AGTTTTTGCT GAGGACTGGA AATGTTTGTC TGAGAGTCCT 
CCTGTCCAAG AAGGCCCAGC GGCCCAGGCT ACCTTTGAGC CCAGCAAGCC ACTCTCCATA 
GCCCACAAAC ACCTGAGCAG GAAGAATGGG CTTTCTAGAC TCTGCCAGAG CCGAATGGCG 
CTCTCTGAGG ACAGGTGGAG CTCTTACTGT CTTTCATCAT TGGCTGCCCA AAACATCTGC 
ACAAGCAAAC TGCACTGCGC TGCTGCACCT GAGTATGCTG ACCCTACAGC TGGGCCACTG 
GGCAGTACTT CCTGCTGCTC CTTGCTGCGT GGCCTGGCTT CAGGATGCTC TGGGTCCCTG 
CTTTCGACCC CTGTGTGCAA CCCCAACAAG GCTGTCTTTA CCGTGGATGC CAAGACCACA 
GAGATTCTGG TTGCCAACGA CAAAGCCTGC AGCCTCCTAG GGTATAGCAG TCATGACCTG 
ATTGGCCAAA AGCTCGCCCA ATTCTTCCTC AAATCTGATT CCGAGGTGGT GGAAGCCCTC 
AGCGAGGAAC ATGTGGAGGC TGATGGCCAC GCTGCAGTGG TGTTCGGCAC AGTGGTGGAC 
ATCGTCAGCC GAATTGGGGA GAAGATTCCA GTCTCTGTGT GGATAAAGAG ATTGCAGCAA 
GATCGTGGCT TGTGCTGTGT AGTGGTTCTG GAGCCTGTGG AGAGAGTGTC AGCCTGGGTC 
GCCTTCCAGA GTGATGGAAC TATCACATCA TGCGACAGTC TCTTTGCTCA TCTCCATGGG 
TTCACATCTC CAAAGGATGT AGTTGGGCAG TGTGTCATAG ACTTGATTCC ATCCATGCAG 
CTCCCTCCTC CTGGCCAACA TATCCCAAAG AGTCTCAAGA TTCAAAGGTC TGTTGGGAGA 
GCCAGAGACG GCACCACCTT CCCTCTCAGT TTGAAGCTGA AATCCAAGCC CAGTGGAAGG 
GCTGTAGCTG ACAGTGAGGC GGCCTCTGAA CCAGGTTACC AGGCTTCTGT GTGGGTGTTC 
TGCACCATCA GTGGCCTCAT CACTCTGCTG CCAGATGGCA CAATTTATGG AGTCAACCAC 
AGCTTTGCAC TAATGCTGTT TGGTTATGGA AAGACAGAGC TCCTGGGCAA GAATATCACT 
TTCCTGATTC CTGGTTTCTA CCACTACATG GACCTCACAT ATGATAGTTC GGTACAGCTG 
CCAGATCTGG TCAATTGCCT CGACATTGGC AGAAAGAGTG GGCCTGGGGA GATGAACTCT 
GATGCCCAAC ACAACTGGGA ACTGGCCAGT GGGGCCCAGG GTCCAAGAAT TGACGTGGTG 
TTGGCACGGG ACCACATGCC ATCACAAGAT GAGACCCTGA AGCTGGTGGG AGGACAAGTC 
TCCTCTAGGA CTCAGACACG ATTGGAAACT GGATACAAGA TACTTCCTTC TTCTGCATGT 
CAGCCCTCAC TGGGGGTGGA CAGCAACCCA GAGGATGGTG AGCAGTCATT GCTCACAGAC 
CAGCAAAGCA TTCCAAAGAG AAACCTGCCA GCCCATGGTG GTCAGAACCA GCTAGACACC 
TCAGAAATAT CCCTACCAGT CCTTAAAGAA CATTTATTGT CTGAAATTCA GAAAAATATC 
TCAGAAGAAA GCCCACTAAC CCACAGGAAA TGGTTATCCA AGGTGCAACA AAACCCCACA 
AAAGGAAGCC TGCCCATCCA CGAGGAGCAG CTATTGTTTG CAGGCCAGCA TATCCATGTC 
TTGGGAAAGG AGGATCCTTC AGCAGCAGAG AGCTACAGGG AGAGTCTTTT AGAAGAAAGC 
AAGTCCAAAC CAGTGGATGC AAAACTGTTT GCTTCATGTG AAGATTCTGA GCCTCTAGTT 
TCAGTTAAGG ACAGGGGCAG CAGTGTGGAC ACGTGTAATC TGCATCAGGA AGCTCAGTTG 
GAGCTGATGG GTGTCAGCAG TCCTAACCCC TGGGCTGATG CCACCATGCC TGAGCCCCAC 
ACCACAGGGC AGATAGCAGG CGGGAGCCTT ACATATTGCC CACAGTACAG AAGCGAGTGG 
GCCTCACAGC AGCGAGGCCA GGACTCGGCT CCCAGCCCCT CTGGAATGGC GTGTGTCTTG 
CTTGGGACAC CCACTCTGGA TGAACCATGG CCAGGAGTGA GAAATGACAG AGAAGAGCTG 
CAGACTTGCT TGATTAAGGA ACAGTTATCC AAGTCAAGCT GTGAGGGAAA TCTGGGCATC 
TCCCGTGTGG AACTCGTTCC TGAAGAACAT CCTCCTTTCA CCGCCCCCGT GTCTTTCTGT 
GATCTGGGAG GCCGAGATCT ACACGCTAGC CGCTCAGGCA GCTCCTCAGC CTGCTATGCT 
TTGGCCACCG ACCTCCCTGG TGTCCTGGAG GCAGTGGAAG CTCAGGAGGC TGATGTGAAT 
TCTTATTCCT GGAACCTCAA GGAGCTCTTT CTCAAGGACC AGACAGACAG AACTCCATCA 
CATTGTTCCT GTACCACATC TGAACTCAGC GAGGCGCCAT CTCTTTCAGT GGTGGGGTCA 
GATTTAGATG TGGGTATTCT GCATAGGCAG ACTTCAGATA TTTTAGTTGA CCGAGAGATG 
TTGCTCCTTA CTGGGACCTA TTTTGACCTT AGTGAAGGCC AACGGTTCCA GGAGATGGGA 
GCAGGACATG ATCGAGCAGA ACTGTCTAAC ATTTCTTTGG TATCCTCTGA ACACTATGAA 
ACTAGTGACA TAGAAAGCCC AGGTTGTGAC CCTCCCTTAC CAGATCCTGG TCCCAACGAT 
ATGTGCCTAT CAGCAGAGAA GCCCAGGCCG AGTGCCCAGA TCACTTCCAC TCCTGTGGCA 
CGTGGGGCCA CCAGCCTGCA GCAGGAGATC CAAGAGGGCA TCTACTCTGG AAGCTGCTAC 
CACCGGGATG GCTTACAACT GAGTATACAG TTTGAAGTCA AGCGTGTGGA GCTCCAGGGC 
TCTGCGACCC TGTTCTGCTG CTGGCTGGTG AAGGACCTCT TCCATAGCCA CCGGGACTCA 
GCCACACGAA CCCGCCTGTT CCTTGCCAGT CTGCCTAGCT CTACCCACTC CATGCCTGAG 
CTGTCTGGAT CCAGCTTTGG AGAGGTGCTC AGAGCCAAGC CTTGGTTTGA GGAATCCCCA 
ACACCTGCAG AGCTAGAGGG GCTGGCGGCC TGTGAGGGCG AGTACGACTA TAAGTATAAC 
ACCATCAGCC CACTTGGCAG TGGGGCCTTT GGCTTTGTGT GGACTGCAGT GGAGAAGGAA 
TGCAACAAAG AGGTGGTGGT GAAGTTTATT AAGAAGGAGA AGGTTTTAGA GGATTGCTGG 
ATTGAGGATC CCAAACTTGG AAGAGTTACT TTAGAGATTG CAATTCTGTC CAAGGTGGAC 
CATGCCAACA TAATCAAGGT TCTGGATATA TTCGAAAACC AAGAGTTCTT CCAGCTGGTG 
ATGGAAAAGC ACGGCTCTGG CATGGACCTC TTTGCTTTCA TTGACCACCA TCCCTGCTTG 
GATGAGCCCC TGGCGAGTTT CATCTTTCGA CAACTAGTGT CTGCTGTAGG ATACCTGCAC 
TCCCAAGGCA TCATCCATAG AGACATCAAG GATGAGAACA TTGTGATTGC TGAGGACTTC 
ACAATTAAGC TGATAGATTT TGGCTCAGCT GCCTACTTAG AGAGGGGCAA ACTATTTTAT 
ACCTTTTGTG GAACAATCGA ATACTGTGCA CCTGAGGTTC TCATTGGAAA TCCCTACAGA 
GGGCCAGAGC TGGAGATGTG GTCTCTGGGG GTCACCCTGT ACACGCTCAT CTTCGAGGAG 
AATCCCTTCT GTGAGGTGGA GGAGACCATG GAGGCAGTTA TTCATCCCCC ATTCCTGGTT 
TCCCAAGAAC TTATGAGTCT TCTGTCTGGA CTGCTGCAGC CTTGCCCTGA GCAGCGGACC 
ACTTTGGAGA AGCTGATCAG GGACCCCTGG GTGACACAGC CTGTGAACCT TGCTAGCTAT 
ACTTGGGAAG AGGTGTGTAG GACCAACCAG CCAGAAAGTG GCCTGCTGTC AGCTGCAAGT 
CTGGAGATTG GGAGTAGGAG TCCAAGTGAA ATGGCTCAGA GAGAGGGTCT CTGTGGGCCT 
CCTGCTCCCA GGGAGACTCG TGGTGACCAG CACTGCTTGC ATCTTAAGGA CCCCTCTTTG 
CCAGTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cell type-specific deletion in mice reveals roles for PAS kinase in insulin and glucagon production.
Diabetologia59(9)1938-47(2016 Sep)
Semplici F,Mondragon A,Macintyre B,Madeyski-Bengston K,Persson-Kry A,Barr S,Ramne A,Marley A,McGinty J,French P,Soedling H,Yokosuka R,Gaitan J,Lang J,Migrenne-Li S,Philippe E,Herrera PL,Magnan C,da Silva Xavier G,Rutter GA

Pask integrates hormonal signaling with histone modification via Wdr5 phosphorylation to drive myogenesis.
eLife5(2016 Sep)
Kikani CK,Wu X,Paul L,Sabic H,Shen Z,Shakya A,Keefe A,Villanueva C,Kardon G,Graves B,Tantin D,Rutter J

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03-6811-6572

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03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

Pask cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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