Cyb5r4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cyb5r4
Entrez Gene ID	266690
Full Name	cytochrome b5 reductase 4
Synonyms	2810034J18Rik,B5+B5R,C79736,Cb5cb5r,Ncb5or,b5/b5r,b5b5r
General protein information	Preferred Names cytochrome b5 reductase 4 Names cytochrome b5 reductase 4 N-terminal cytochrome b5 and cytochrome b5 oxidoreductase domain-containing protein NADPH cytochrome B5 oxidoreductase cb5/cb5R cytochrome b-type NAD(P)H oxidoreductase flavohemoprotein b5/b5R
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 E3.1

mRNA and Protein(s)

mRNA	Protein	Name
XM_030244394.1	XP_030100254.1	cytochrome b5 reductase 4 isoform X3
NM_024195.2	NP_077157.2	cytochrome b5 reductase 4 isoform 1
XM_006511199.1	XP_006511262.1	cytochrome b5 reductase 4 isoform X2
NM_001359962.1	NP_001346891.1	cytochrome b5 reductase 4 isoform 2
XM_017313397.1	XP_017168886.1	cytochrome b5 reductase 4 isoform X1
XM_006511200.2	XP_006511263.1	cytochrome b5 reductase 4 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CYB5R4	XP_518614.3
Bos taurus (cattle)	CYB5R4	NP_001033248.1
Gallus gallus (chicken)	CYB5R4	XP_001233871.1
	CYB5R4	XP_001085835.1
Canis lupus familiaris (dog)	CYB5R4	XP_532219.3
Mus musculus (house mouse)	Cyb5r4	NP_077157.2
Rattus norvegicus (Norway rat)	Cyb5r4	NP_596918.3
Danio rerio (zebrafish)	cyb5r4	NP_001018496.1
Xenopus tropicalis (tropical clawed frog)	cyb5r4	NP_001016756.1
Drosophila melanogaster (fruit fly)	CG11257	NP_611419.1
Caenorhabditis elegans (roundworm)	CELE_Y52B11A.3	NP_001021784.2
Homo sapiens (human)	CYB5R4	NP_057314.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011314	XP_309336.4

Gene Ontology
Bibliography

Related articles in PubMed

NCB5OR Deficiency in the Cerebellum and Midbrain Leads to Dehydration and Alterations in Thirst Response, Fasted Feeding Behavior, and Voluntary Exercise in Mice.
Stroh MA, Winter MK, McCarson KE, Thyfault JP, Zhu H
Cerebellum (London, England)(2017 Sep)

Loss of NCB5OR in the cerebellum disturbs iron pathways, potentiates behavioral abnormalities, and exacerbates harmaline-induced tremor in mice.
Stroh MA, Winter MK, Swerdlow RH, McCarson KE, Zhu H
Metabolic brain disease31(4)951-64(2016 Aug)

Cytochrome b5 and epoxide hydrolase contribute to benzo[a]pyrene-DNA adduct formation catalyzed by cytochrome P450 1A1 under low NADPH:P450 oxidoreductase conditions.
Stiborov? M, Moserov? M, ?ern? V, Indra R, Dra??nsk? M, ?ulc M, Henderson CJ, Wolf CR, Schmeiser HH, Phillips DH, Frei E, Arlt VM
Toxicology3181-12(2014 Apr)

Development of diabetes in lean Ncb5or-null mice is associated with manifestations of endoplasmic reticulum and oxidative stress in beta cells.
Wang W, Guo Y, Xu M, Huang HH, Novikova L, Larade K, Jiang ZG, Thayer TC, Frontera JR, Aires D, Ding H, Turk J, Mathews CE, Bunn HF, Stehno-Bittel L, Zhu H
Biochimica et biophysica acta1812(11)1532-41(2011 Nov)

Ncb5or deficiency increases fatty acid catabolism and oxidative stress.
Xu M, Wang W, Frontera JR, Neely MC, Lu J, Aires D, Hsu FF, Turk J, Swerdlow RH, Carlson SE, Zhu H
The Journal of biological chemistry286(13)11141-54(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our findings suggest an essential role for NCB5OR in maintaining both iron homeostasis and the proper functioning of various locomotor pathways in the mouse cerebellum and midbrain.
Title: Loss of NCB5OR in the cerebellum disturbs iron pathways, potentiates behavioral abnormalities, and exacerbates harmaline-induced tremor in mice.

In livers of HRN mice Cyp1a1, cytochrome b5 and mEH can effectively activate BaP to DNA binding species, even in the presence of very low amounts of P450 oxidoreductase.
Title: Cytochrome b5 and epoxide hydrolase contribute to benzo[a]pyrene-DNA adduct formation catalyzed by cytochrome P450 1A1 under low NADPH:P450 oxidoreductase conditions.

FFA accumulation and catabolism and oxidative stress are major consequences of Ncb5or deficiency in liver.
Title: Ncb5or deficiency increases fatty acid catabolism and oxidative stress.

Study of the individual cytochrome b5 and cytochrome b5 reductase domains of Ncb5or reveals a unique heme pocket and a possible role of the CS domain.
Title: Study of the individual cytochrome b5 and cytochrome b5 reductase domains of Ncb5or reveals a unique heme pocket and a possible role of the CS domain.

Onset of hyperglycemia in the Chop(-/-);Ncb5or(-/-) mice was delayed two weeks beyond that observed in Chop(+/+);Ncb5or(-/-) mice.
Title: The flavoheme reductase Ncb5or protects cells against endoplasmic reticulum stress-induced lipotoxicity.

The following Cyb5r4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cyb5r4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu22447	NM_024195.2 Latest version!	Mus musculus cytochrome b5 reductase 4 (Cyb5r4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu42811	NM_001359962.1 Latest version!	Mus musculus cytochrome b5 reductase 4 (Cyb5r4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu42811	XM_006511199.1 Latest version!	Mus musculus cytochrome b5 reductase 4 (Cyb5r4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu42812	XM_006511200.2 Latest version!	Mus musculus cytochrome b5 reductase 4 (Cyb5r4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu90189	XM_030244394.1 Latest version!	Mus musculus cytochrome b5 reductase 4 (Cyb5r4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu82830	XM_017313397.1 Latest version!	Mus musculus cytochrome b5 reductase 4 (Cyb5r4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cyb5r4 ( NM_024195.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_077157.2 Mus musculus cytochrome b5 reductase 4 (Cyb5r4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu22447
Clone ID Related Accession (Same CDS sequence)	NM_024195.2
Accession Version	NM_024195.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	cytochrome b5 reductase 4 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK090159.1 and CO801035.1. On Jul 19, 2008 this sequence version replaced NM_024195.1. CCDS Note: The coding region has been updated to extend the N-terminus to one that is more supported by available conservation data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK090159.1, AK157312.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGAACG TCCCTTCCCA GGCTTTCCCG GCCCCCGGCT CCCAGCAGCG CGTCTCCTCC 
CAGGGGCGCA GCAAGGTACC TTTAAAACAA GGCAGGAGTC TTATGGATTG GATCCGACTG 
ACCAAGAGTG GAAAGGACTT AACAGGATTG AAGGGCGGGT TAATTGAAGT AACTGAAGAA 
GAGCTTAAGA AGCACAACAA GAAAGAAGAT TGTTGGATAT GCATAAGAGG GTTTGTTTAC 
AACGTCAGCC CTTATATGGA GTACCATCCG GGTGGGGAGG ATGAACTCAT GAGAGCCGCA 
GGAGCCGATG GAACAGATCT TTTTAATGAG GTTCATCGTT GGGTCAATTA TGAATCCATG 
CTGAAAGAAT GCCTGGTTGG CAGGATGGCT GTGAAGCCTG CTGTTCCAAA AGACTGTCAT 
GAAGGAAAGA GAGTCTTAAA TGGCATGCTT CCCAAGAGCC AAATGTCAGA CACACTGCCC 
AGGGATGTGA CAGACACACT GCCCAGGGAA GACCTCAGTT CTCCAAGCTA TGATTGGTTC 
CAAACAGAGT CTTCAGTCAC CATTGTTGTA TACACCAAAC AAAAGAATAT CAGTTTAGAC 
TCAGTAATCG TTGACCTCCA GGATGACTCC TTGAGAGCAG AAGCAGTCAT CAAGGATCAC 
TCTTACCTTG TACATGTTGG GCTAAGCCAT GAAGTTCAAG AAAATTTTTC TGTACGGGTT 
ATTGAAAATG TTGGAAAAAT AGAGATTGTC CTACAAAAGA AAGAAAGTGT TTCTTGGCAA 
TGTCTTGGTG ATCACCTGGA GAAACATGAT TCTTTCATTC CAAAGAAAGA TACAGGCTTG 
TACTACAGAC GGTGCCAGTT AATATCTAAG GAAGATGTCA CTCATGACAC GAGACTTTTC 
TGTCTGATGC TGCCTCCAAG TACTCACCTT CAGGTGCCAG TTGGACAACA TGTTTACCTC 
AAGCTCAGTG TCACAGGTGC AGAAATAGTA AAGCCATATA CACCCGTGTC TGATTCCTTA 
CTCTCAGATT TTAAAGAACC AGTCCTTTCC CCAAATAAAT ACATCTGCTT TTTGATCAAA 
ATCTATCCTG CTGGACTCTT CACACCAGAG CTTGATCGTC TTCAGATTGG AGATTTTATT 
TCTGTAAGCG GACCTGAAGG CGATTTTAAA GTATCCAAAC TCCAGGAAGT AGAAGATCTC 
TTTCTATTGG CAGCCGGAAC AGGATTCACA CCAATGGTCA CTGTACTGAA TTATGCTTTG 
TCTCACATGT CCAGTCTCAG GAAGGTGAAG TTGATGTTCT TCAATAAAAC AGAGGACGAT 
ATAATTTGGA GATGTCAGCT AGAGAAGTTG GCATTAAGAG AAAAAAGATT TGATGTTGAA 
TTTGTTCTCT CGGCACCTTC TCCAGAATGG AATGGCAAAC AGGGACACAT ATCACGAGCT 
CTTCTCTCTG AATTTTTGCA AAGAAGTTCA GAAAACTCTA GGGCCTTCCT TTGCATCTGC 
GGACCAACAC CATTTACTGA CGAGGGGATA AGGTTGCTGC ATGATCTTAA CTTTTCTGAT 
GATGAGATTC ACGGATTCAC CGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077157.2
CDS	151..1737
Translation	MLNVPSQAFPAPGSQQRVSSQGRSKVPLKQGRSLMDWIRLTKSGKDLTGLKGGLIEVTEEELKKHNKKEDCWICIRGFVYNVSPYMEYHPGGEDELMRAAGADGTDLFNEVHRWVNYESMLKECLVGRMAVKPAVPKDCHEGKRVLNGMLPKSQMSDTLPRDVTDTLPREDLSSPSYDWFQTESSVTIVVYTKQKNISLDSVIVDLQDDSLRAEAVIKDHSYLVHVGLSHEVQENFSVRVIENVGKIEIVLQKKESVSWQCLGDHLEKHDSFIPKKDTGLYYRRCQLISKEDVTHDTRLFCLMLPPSTHLQVPVGQHVYLKLSVTGAEIVKPYTPVSDSLLSDFKEPVLSPNKYICFLIKIYPAGLFTPELDRLQIGDFISVSGPEGDFKVSKLQEVEDLFLLAAGTGFTPMVTVLNYALSHMSSLRKVKLMFFNKTEDDIIWRCQLEKLALREKRFDVEFVLSAPSPEWNGKQGHISRALLSEFLQRSSENSRAFLCICGPTPFTDEGIRLLHDLNFSDDEIHGFTA

Target ORF information:

RefSeq Version	NM_024195.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytochrome b5 reductase 4 (Cyb5r4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024195.2

ORF Insert Sequence:

ATGCTGAACG TCCCTTCCCA GGCTTTCCCG GCCCCCGGCT CCCAGCAGCG CGTCTCCTCC 
CAGGGGCGCA GCAAGGTACC TTTAAAACAA GGCAGGAGTC TTATGGATTG GATCCGACTG 
ACCAAGAGTG GAAAGGACTT AACAGGATTG AAGGGCGGGT TAATTGAAGT AACTGAAGAA 
GAGCTTAAGA AGCACAACAA GAAAGAAGAT TGTTGGATAT GCATAAGAGG GTTTGTTTAC 
AACGTCAGCC CTTATATGGA GTACCATCCG GGTGGGGAGG ATGAACTCAT GAGAGCCGCA 
GGAGCCGATG GAACAGATCT TTTTAATGAG GTTCATCGTT GGGTCAATTA TGAATCCATG 
CTGAAAGAAT GCCTGGTTGG CAGGATGGCT GTGAAGCCTG CTGTTCCAAA AGACTGTCAT 
GAAGGAAAGA GAGTCTTAAA TGGCATGCTT CCCAAGAGCC AAATGTCAGA CACACTGCCC 
AGGGATGTGA CAGACACACT GCCCAGGGAA GACCTCAGTT CTCCAAGCTA TGATTGGTTC 
CAAACAGAGT CTTCAGTCAC CATTGTTGTA TACACCAAAC AAAAGAATAT CAGTTTAGAC 
TCAGTAATCG TTGACCTCCA GGATGACTCC TTGAGAGCAG AAGCAGTCAT CAAGGATCAC 
TCTTACCTTG TACATGTTGG GCTAAGCCAT GAAGTTCAAG AAAATTTTTC TGTACGGGTT 
ATTGAAAATG TTGGAAAAAT AGAGATTGTC CTACAAAAGA AAGAAAGTGT TTCTTGGCAA 
TGTCTTGGTG ATCACCTGGA GAAACATGAT TCTTTCATTC CAAAGAAAGA TACAGGCTTG 
TACTACAGAC GGTGCCAGTT AATATCTAAG GAAGATGTCA CTCATGACAC GAGACTTTTC 
TGTCTGATGC TGCCTCCAAG TACTCACCTT CAGGTGCCAG TTGGACAACA TGTTTACCTC 
AAGCTCAGTG TCACAGGTGC AGAAATAGTA AAGCCATATA CACCCGTGTC TGATTCCTTA 
CTCTCAGATT TTAAAGAACC AGTCCTTTCC CCAAATAAAT ACATCTGCTT TTTGATCAAA 
ATCTATCCTG CTGGACTCTT CACACCAGAG CTTGATCGTC TTCAGATTGG AGATTTTATT 
TCTGTAAGCG GACCTGAAGG CGATTTTAAA GTATCCAAAC TCCAGGAAGT AGAAGATCTC 
TTTCTATTGG CAGCCGGAAC AGGATTCACA CCAATGGTCA CTGTACTGAA TTATGCTTTG 
TCTCACATGT CCAGTCTCAG GAAGGTGAAG TTGATGTTCT TCAATAAAAC AGAGGACGAT 
ATAATTTGGA GATGTCAGCT AGAGAAGTTG GCATTAAGAG AAAAAAGATT TGATGTTGAA 
TTTGTTCTCT CGGCACCTTC TCCAGAATGG AATGGCAAAC AGGGACACAT ATCACGAGCT 
CTTCTCTCTG AATTTTTGCA AAGAAGTTCA GAAAACTCTA GGGCCTTCCT TTGCATCTGC 
GGACCAACAC CATTTACTGA CGAGGGGATA AGGTTGCTGC ATGATCTTAA CTTTTCTGAT 
GATGAGATTC ACGGATTCAC CGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cyb5r4 ( NM_001359962.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346891.1 Mus musculus cytochrome b5 reductase 4 (Cyb5r4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu42811
Clone ID Related Accession (Same CDS sequence)	XM_006511199.1 , NM_001359962.1
Accession Version	NM_001359962.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-01
Organism	Mus musculus(house mouse)
Product	cytochrome b5 reductase 4 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC156793.3 and CO801035.1. On Jan 20, 2018 this sequence version replaced XM_006511199.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.163428.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGAACG TCCCTTCCCA GGCTTTCCCG GCCCCCGGCT CCCAGCAGCG CGTCTCCTCC 
CAGGGGCGCA GCAAGGTACC TTTAAAACAA GGCAGGAGTC TTATGGATTG GATCCGACTG 
ACCAAGAGTG GAAAGGACTT AACAGGATTG AAGGGCGGGT TAATTGAAGT AACTGAAGAA 
GAGCTTAAGA AGCACAACAA GAAAGAAGAT TGTTGGATAT GCATAAGAGG GTTTGTTTAC 
AACGTCAGCC CTTATATGGA GTACCATCCG GGTGGGGAGG ATGAACTCAT GAGAGCCGCA 
GGAGCCGATG GAACAGATCT TTTTAATGAG GTTCATCGTT GGGTCAATTA TGAATCCATG 
CTGAAAGAAT GCCTGGTTGG CAGGATGGCT GTGAAGCCTG CTGTTCCAAA AGACTGTCAT 
GAAGGAAAGA GAGTCTTAAA TGGCATGCTT CCCAAGAGCC AAATGTCAGA CACACTGCCC 
AGGGATGTGA CAGACACACT GCCCAGGGAA GACCTCAGTT CTCCAAGCTA TGATTGGTTC 
CAAACAGAGT CTTCAGTCAC CATTGTTGTA TACACCAAAC AAAAGAATAT CAGTTTAGAC 
TCAGTAATCG TTGACCTCCA GGATGACTCC TTGAGAGCAG AAGCAGTCAT CAAGGATCAC 
TCTTACCTTG TACATGTTGG GCTAAGCCAT GAAGTTCAAG AAAATTTTTC TGTACGGGTT 
ATTGAAAATG TTGGAAAAAT AGAGATTGTC CTACAAAAGA AAGAAAGTGT TTCTTGGCAA 
TGTCTTGGTG ATCACCTGGA GAAACATGAT TCTTTCATTC CAAAGAAAGA TACAGGCTTG 
TACTACAGAC GGTGCCAGTT AATATCTAAG GAAGATGTCA CTCATGACAC GAGACTTTTC 
TGTCTGATGC TGCCTCCAAG TACTCACCTT CAGGTGCCAG TTGGACAACA TGTTTACCTC 
AAGCTCAGTG TCACAGGAGA TTTTATTTCT GTAAGCGGAC CTGAAGGCGA TTTTAAAGTA 
TCCAAACTCC AGGAAGTAGA AGATCTCTTT CTATTGGCAG CCGGAACAGG ATTCACACCA 
ATGGTCACTG TACTGAATTA TGCTTTGTCT CACATGTCCA GTCTCAGGAA GGTGAAGTTG 
ATGTTCTTCA ATAAAACAGA GGACGATATA ATTTGGAGAT GTCAGCTAGA GAAGTTGGCA 
TTAAGAGAAA AAAGATTTGA TGTTGAATTT GTTCTCTCGG CACCTTCTCC AGAATGGAAT 
GGCAAACAGG GACACATATC ACGAGCTCTT CTCTCTGAAT TTTTGCAAAG AAGTTCAGAA 
AACTCTAGGG CCTTCCTTTG CATCTGCGGA CCAACACCAT TTACTGACGA GGGGATAAGG 
TTGCTGCATG ATCTTAACTT TTCTGATGAT GAGATTCACG GATTCACCGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346891.1
CDS	151..1584
Translation	MLNVPSQAFPAPGSQQRVSSQGRSKVPLKQGRSLMDWIRLTKSGKDLTGLKGGLIEVTEEELKKHNKKEDCWICIRGFVYNVSPYMEYHPGGEDELMRAAGADGTDLFNEVHRWVNYESMLKECLVGRMAVKPAVPKDCHEGKRVLNGMLPKSQMSDTLPRDVTDTLPREDLSSPSYDWFQTESSVTIVVYTKQKNISLDSVIVDLQDDSLRAEAVIKDHSYLVHVGLSHEVQENFSVRVIENVGKIEIVLQKKESVSWQCLGDHLEKHDSFIPKKDTGLYYRRCQLISKEDVTHDTRLFCLMLPPSTHLQVPVGQHVYLKLSVTGDFISVSGPEGDFKVSKLQEVEDLFLLAAGTGFTPMVTVLNYALSHMSSLRKVKLMFFNKTEDDIIWRCQLEKLALREKRFDVEFVLSAPSPEWNGKQGHISRALLSEFLQRSSENSRAFLCICGPTPFTDEGIRLLHDLNFSDDEIHGFTA

Target ORF information:

RefSeq Version	NM_001359962.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytochrome b5 reductase 4 (Cyb5r4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359962.1

ORF Insert Sequence:

ATGCTGAACG TCCCTTCCCA GGCTTTCCCG GCCCCCGGCT CCCAGCAGCG CGTCTCCTCC 
CAGGGGCGCA GCAAGGTACC TTTAAAACAA GGCAGGAGTC TTATGGATTG GATCCGACTG 
ACCAAGAGTG GAAAGGACTT AACAGGATTG AAGGGCGGGT TAATTGAAGT AACTGAAGAA 
GAGCTTAAGA AGCACAACAA GAAAGAAGAT TGTTGGATAT GCATAAGAGG GTTTGTTTAC 
AACGTCAGCC CTTATATGGA GTACCATCCG GGTGGGGAGG ATGAACTCAT GAGAGCCGCA 
GGAGCCGATG GAACAGATCT TTTTAATGAG GTTCATCGTT GGGTCAATTA TGAATCCATG 
CTGAAAGAAT GCCTGGTTGG CAGGATGGCT GTGAAGCCTG CTGTTCCAAA AGACTGTCAT 
GAAGGAAAGA GAGTCTTAAA TGGCATGCTT CCCAAGAGCC AAATGTCAGA CACACTGCCC 
AGGGATGTGA CAGACACACT GCCCAGGGAA GACCTCAGTT CTCCAAGCTA TGATTGGTTC 
CAAACAGAGT CTTCAGTCAC CATTGTTGTA TACACCAAAC AAAAGAATAT CAGTTTAGAC 
TCAGTAATCG TTGACCTCCA GGATGACTCC TTGAGAGCAG AAGCAGTCAT CAAGGATCAC 
TCTTACCTTG TACATGTTGG GCTAAGCCAT GAAGTTCAAG AAAATTTTTC TGTACGGGTT 
ATTGAAAATG TTGGAAAAAT AGAGATTGTC CTACAAAAGA AAGAAAGTGT TTCTTGGCAA 
TGTCTTGGTG ATCACCTGGA GAAACATGAT TCTTTCATTC CAAAGAAAGA TACAGGCTTG 
TACTACAGAC GGTGCCAGTT AATATCTAAG GAAGATGTCA CTCATGACAC GAGACTTTTC 
TGTCTGATGC TGCCTCCAAG TACTCACCTT CAGGTGCCAG TTGGACAACA TGTTTACCTC 
AAGCTCAGTG TCACAGGAGA TTTTATTTCT GTAAGCGGAC CTGAAGGCGA TTTTAAAGTA 
TCCAAACTCC AGGAAGTAGA AGATCTCTTT CTATTGGCAG CCGGAACAGG ATTCACACCA 
ATGGTCACTG TACTGAATTA TGCTTTGTCT CACATGTCCA GTCTCAGGAA GGTGAAGTTG 
ATGTTCTTCA ATAAAACAGA GGACGATATA ATTTGGAGAT GTCAGCTAGA GAAGTTGGCA 
TTAAGAGAAA AAAGATTTGA TGTTGAATTT GTTCTCTCGG CACCTTCTCC AGAATGGAAT 
GGCAAACAGG GACACATATC ACGAGCTCTT CTCTCTGAAT TTTTGCAAAG AAGTTCAGAA 
AACTCTAGGG CCTTCCTTTG CATCTGCGGA CCAACACCAT TTACTGACGA GGGGATAAGG 
TTGCTGCATG ATCTTAACTT TTCTGATGAT GAGATTCACG GATTCACCGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cyb5r4 ( XM_006511199.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511262.1 Mus musculus cytochrome b5 reductase 4 (Cyb5r4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu42811
Clone ID Related Accession (Same CDS sequence)	XM_006511199.1 , NM_001359962.1
Accession Version	XM_006511199.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	cytochrome b5 reductase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAACG TCCCTTCCCA GGCTTTCCCG GCCCCCGGCT CCCAGCAGCG CGTCTCCTCC 
CAGGGGCGCA GCAAGGTACC TTTAAAACAA GGCAGGAGTC TTATGGATTG GATCCGACTG 
ACCAAGAGTG GAAAGGACTT AACAGGATTG AAGGGCGGGT TAATTGAAGT AACTGAAGAA 
GAGCTTAAGA AGCACAACAA GAAAGAAGAT TGTTGGATAT GCATAAGAGG GTTTGTTTAC 
AACGTCAGCC CTTATATGGA GTACCATCCG GGTGGGGAGG ATGAACTCAT GAGAGCCGCA 
GGAGCCGATG GAACAGATCT TTTTAATGAG GTTCATCGTT GGGTCAATTA TGAATCCATG 
CTGAAAGAAT GCCTGGTTGG CAGGATGGCT GTGAAGCCTG CTGTTCCAAA AGACTGTCAT 
GAAGGAAAGA GAGTCTTAAA TGGCATGCTT CCCAAGAGCC AAATGTCAGA CACACTGCCC 
AGGGATGTGA CAGACACACT GCCCAGGGAA GACCTCAGTT CTCCAAGCTA TGATTGGTTC 
CAAACAGAGT CTTCAGTCAC CATTGTTGTA TACACCAAAC AAAAGAATAT CAGTTTAGAC 
TCAGTAATCG TTGACCTCCA GGATGACTCC TTGAGAGCAG AAGCAGTCAT CAAGGATCAC 
TCTTACCTTG TACATGTTGG GCTAAGCCAT GAAGTTCAAG AAAATTTTTC TGTACGGGTT 
ATTGAAAATG TTGGAAAAAT AGAGATTGTC CTACAAAAGA AAGAAAGTGT TTCTTGGCAA 
TGTCTTGGTG ATCACCTGGA GAAACATGAT TCTTTCATTC CAAAGAAAGA TACAGGCTTG 
TACTACAGAC GGTGCCAGTT AATATCTAAG GAAGATGTCA CTCATGACAC GAGACTTTTC 
TGTCTGATGC TGCCTCCAAG TACTCACCTT CAGGTGCCAG TTGGACAACA TGTTTACCTC 
AAGCTCAGTG TCACAGGAGA TTTTATTTCT GTAAGCGGAC CTGAAGGCGA TTTTAAAGTA 
TCCAAACTCC AGGAAGTAGA AGATCTCTTT CTATTGGCAG CCGGAACAGG ATTCACACCA 
ATGGTCACTG TACTGAATTA TGCTTTGTCT CACATGTCCA GTCTCAGGAA GGTGAAGTTG 
ATGTTCTTCA ATAAAACAGA GGACGATATA ATTTGGAGAT GTCAGCTAGA GAAGTTGGCA 
TTAAGAGAAA AAAGATTTGA TGTTGAATTT GTTCTCTCGG CACCTTCTCC AGAATGGAAT 
GGCAAACAGG GACACATATC ACGAGCTCTT CTCTCTGAAT TTTTGCAAAG AAGTTCAGAA 
AACTCTAGGG CCTTCCTTTG CATCTGCGGA CCAACACCAT TTACTGACGA GGGGATAAGG 
TTGCTGCATG ATCTTAACTT TTCTGATGAT GAGATTCACG GATTCACCGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511262.1
CDS	172..1605
Translation	MLNVPSQAFPAPGSQQRVSSQGRSKVPLKQGRSLMDWIRLTKSGKDLTGLKGGLIEVTEEELKKHNKKEDCWICIRGFVYNVSPYMEYHPGGEDELMRAAGADGTDLFNEVHRWVNYESMLKECLVGRMAVKPAVPKDCHEGKRVLNGMLPKSQMSDTLPRDVTDTLPREDLSSPSYDWFQTESSVTIVVYTKQKNISLDSVIVDLQDDSLRAEAVIKDHSYLVHVGLSHEVQENFSVRVIENVGKIEIVLQKKESVSWQCLGDHLEKHDSFIPKKDTGLYYRRCQLISKEDVTHDTRLFCLMLPPSTHLQVPVGQHVYLKLSVTGDFISVSGPEGDFKVSKLQEVEDLFLLAAGTGFTPMVTVLNYALSHMSSLRKVKLMFFNKTEDDIIWRCQLEKLALREKRFDVEFVLSAPSPEWNGKQGHISRALLSEFLQRSSENSRAFLCICGPTPFTDEGIRLLHDLNFSDDEIHGFTA

Target ORF information:

RefSeq Version	XM_006511199.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytochrome b5 reductase 4 (Cyb5r4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511199.1

ORF Insert Sequence:

ATGCTGAACG TCCCTTCCCA GGCTTTCCCG GCCCCCGGCT CCCAGCAGCG CGTCTCCTCC 
CAGGGGCGCA GCAAGGTACC TTTAAAACAA GGCAGGAGTC TTATGGATTG GATCCGACTG 
ACCAAGAGTG GAAAGGACTT AACAGGATTG AAGGGCGGGT TAATTGAAGT AACTGAAGAA 
GAGCTTAAGA AGCACAACAA GAAAGAAGAT TGTTGGATAT GCATAAGAGG GTTTGTTTAC 
AACGTCAGCC CTTATATGGA GTACCATCCG GGTGGGGAGG ATGAACTCAT GAGAGCCGCA 
GGAGCCGATG GAACAGATCT TTTTAATGAG GTTCATCGTT GGGTCAATTA TGAATCCATG 
CTGAAAGAAT GCCTGGTTGG CAGGATGGCT GTGAAGCCTG CTGTTCCAAA AGACTGTCAT 
GAAGGAAAGA GAGTCTTAAA TGGCATGCTT CCCAAGAGCC AAATGTCAGA CACACTGCCC 
AGGGATGTGA CAGACACACT GCCCAGGGAA GACCTCAGTT CTCCAAGCTA TGATTGGTTC 
CAAACAGAGT CTTCAGTCAC CATTGTTGTA TACACCAAAC AAAAGAATAT CAGTTTAGAC 
TCAGTAATCG TTGACCTCCA GGATGACTCC TTGAGAGCAG AAGCAGTCAT CAAGGATCAC 
TCTTACCTTG TACATGTTGG GCTAAGCCAT GAAGTTCAAG AAAATTTTTC TGTACGGGTT 
ATTGAAAATG TTGGAAAAAT AGAGATTGTC CTACAAAAGA AAGAAAGTGT TTCTTGGCAA 
TGTCTTGGTG ATCACCTGGA GAAACATGAT TCTTTCATTC CAAAGAAAGA TACAGGCTTG 
TACTACAGAC GGTGCCAGTT AATATCTAAG GAAGATGTCA CTCATGACAC GAGACTTTTC 
TGTCTGATGC TGCCTCCAAG TACTCACCTT CAGGTGCCAG TTGGACAACA TGTTTACCTC 
AAGCTCAGTG TCACAGGAGA TTTTATTTCT GTAAGCGGAC CTGAAGGCGA TTTTAAAGTA 
TCCAAACTCC AGGAAGTAGA AGATCTCTTT CTATTGGCAG CCGGAACAGG ATTCACACCA 
ATGGTCACTG TACTGAATTA TGCTTTGTCT CACATGTCCA GTCTCAGGAA GGTGAAGTTG 
ATGTTCTTCA ATAAAACAGA GGACGATATA ATTTGGAGAT GTCAGCTAGA GAAGTTGGCA 
TTAAGAGAAA AAAGATTTGA TGTTGAATTT GTTCTCTCGG CACCTTCTCC AGAATGGAAT 
GGCAAACAGG GACACATATC ACGAGCTCTT CTCTCTGAAT TTTTGCAAAG AAGTTCAGAA 
AACTCTAGGG CCTTCCTTTG CATCTGCGGA CCAACACCAT TTACTGACGA GGGGATAAGG 
TTGCTGCATG ATCTTAACTT TTCTGATGAT GAGATTCACG GATTCACCGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cyb5r4 ( XM_006511200.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511263.1 Mus musculus cytochrome b5 reductase 4 (Cyb5r4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu42812
Clone ID Related Accession (Same CDS sequence)	XM_006511200.2
Accession Version	XM_006511200.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cytochrome b5 reductase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006511200.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAACG TCCCTTCCCA GGCTTTCCCG GCCCCCGGCT CCCAGCAGCG CGTCTCCTCC 
CAGGGGCGCA GCAAGGTACC TTTAAAACAA GGCAGGAGTC TTATGGATTG GATCCGACTG 
ACCAAGAGTG GAAAGGACTT AACAGGATTG AAGGGCGGGT TAATTGAAGT AACTGAAGAA 
GAGCTTAAGA AGCACAACAA GAAAGAAGAT TGTTGGATAT GCATAAGAGG GTTTGTTTAC 
AACGTCAGCC CTTATATGGA GTACCATCCG GGTGGGGAGG ATGAACTCAT GAGAGCCGCA 
GGAGCCGATG GAACAGATCT TTTTAATGAG GTTCATCGTT GGGTCAATTA TGAATCCATG 
CTGAAAGAAT GCCTGGTTGG CAGGATGGCT GTGAAGCCTG CTGTTCCAAA AGACTGTCAT 
GAAGGAAAGA GAGTCTTAAA TGGCATGCTT CCCAAGAGCC AAATGTCAGA CACACTGCCC 
AGGGATGTGA CAGACACACT GCCCAGGGAA GACCTCAGTT CTCCAAGCTA TGATTGGTTC 
CAAACAGAGT CTTCAGTCAC CATTGTTGTA TACACCAAAC AAAAGAATAT CAGTTTAGAC 
TCAGTAATCG TTGACCTCCA GGATGACTCC TTGAGAGCAG AAGCAGTCAT CAAGGATCAC 
TCTTACCTTG TACATGTTGG CTTGTACTAC AGACGGTGCC AGTTAATATC TAAGGAAGAT 
GTCACTCATG ACACGAGACT TTTCTGTCTG ATGCTGCCTC CAAGTACTCA CCTTCAGGTG 
CCAGTTGGAC AACATGTTTA CCTCAAGCTC AGTGTCACAG GTGCAGAAAT AGTAAAGCCA 
TATACACCCG TGTCTGATTC CTTACTCTCA GATTTTAAAG AACCAGTCCT TTCCCCAAAT 
AAATACATCT GCTTTTTGAT CAAAATCTAT CCTGCTGGAC TCTTCACACC AGAGCTTGAT 
CGTCTTCAGA TTGGAGATTT TATTTCTGTA AGCGGACCTG AAGGCGATTT TAAAGTATCC 
AAACTCCAGG AAGTAGAAGA TCTCTTTCTA TTGGCAGCCG GAACAGGATT CACACCAATG 
GTCACTGTAC TGAATTATGC TTTGTCTCAC ATGTCCAGTC TCAGGAAGGT GAAGTTGATG 
TTCTTCAATA AAACAGAGGA CGATATAATT TGGAGATGTC AGCTAGAGAA GTTGGCATTA 
AGAGAAAAAA GATTTGATGT TGAATTTGTT CTCTCGGCAC CTTCTCCAGA ATGGAATGGC 
AAACAGGGAC ACATATCACG AGCTCTTCTC TCTGAATTTT TGCAAAGAAG TTCAGAAAAC 
TCTAGGGCCT TCCTTTGCAT CTGCGGACCA ACACCATTTA CTGACGAGGG GATAAGGTTG 
CTGCATGATC TTAACTTTTC TGATGATGAG ATTCACGGAT TCACCGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511263.1
CDS	172..1602
Translation	MLNVPSQAFPAPGSQQRVSSQGRSKVPLKQGRSLMDWIRLTKSGKDLTGLKGGLIEVTEEELKKHNKKEDCWICIRGFVYNVSPYMEYHPGGEDELMRAAGADGTDLFNEVHRWVNYESMLKECLVGRMAVKPAVPKDCHEGKRVLNGMLPKSQMSDTLPRDVTDTLPREDLSSPSYDWFQTESSVTIVVYTKQKNISLDSVIVDLQDDSLRAEAVIKDHSYLVHVGLYYRRCQLISKEDVTHDTRLFCLMLPPSTHLQVPVGQHVYLKLSVTGAEIVKPYTPVSDSLLSDFKEPVLSPNKYICFLIKIYPAGLFTPELDRLQIGDFISVSGPEGDFKVSKLQEVEDLFLLAAGTGFTPMVTVLNYALSHMSSLRKVKLMFFNKTEDDIIWRCQLEKLALREKRFDVEFVLSAPSPEWNGKQGHISRALLSEFLQRSSENSRAFLCICGPTPFTDEGIRLLHDLNFSDDEIHGFTA

Target ORF information:

RefSeq Version	XM_006511200.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytochrome b5 reductase 4 (Cyb5r4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511200.2

ORF Insert Sequence:

ATGCTGAACG TCCCTTCCCA GGCTTTCCCG GCCCCCGGCT CCCAGCAGCG CGTCTCCTCC 
CAGGGGCGCA GCAAGGTACC TTTAAAACAA GGCAGGAGTC TTATGGATTG GATCCGACTG 
ACCAAGAGTG GAAAGGACTT AACAGGATTG AAGGGCGGGT TAATTGAAGT AACTGAAGAA 
GAGCTTAAGA AGCACAACAA GAAAGAAGAT TGTTGGATAT GCATAAGAGG GTTTGTTTAC 
AACGTCAGCC CTTATATGGA GTACCATCCG GGTGGGGAGG ATGAACTCAT GAGAGCCGCA 
GGAGCCGATG GAACAGATCT TTTTAATGAG GTTCATCGTT GGGTCAATTA TGAATCCATG 
CTGAAAGAAT GCCTGGTTGG CAGGATGGCT GTGAAGCCTG CTGTTCCAAA AGACTGTCAT 
GAAGGAAAGA GAGTCTTAAA TGGCATGCTT CCCAAGAGCC AAATGTCAGA CACACTGCCC 
AGGGATGTGA CAGACACACT GCCCAGGGAA GACCTCAGTT CTCCAAGCTA TGATTGGTTC 
CAAACAGAGT CTTCAGTCAC CATTGTTGTA TACACCAAAC AAAAGAATAT CAGTTTAGAC 
TCAGTAATCG TTGACCTCCA GGATGACTCC TTGAGAGCAG AAGCAGTCAT CAAGGATCAC 
TCTTACCTTG TACATGTTGG CTTGTACTAC AGACGGTGCC AGTTAATATC TAAGGAAGAT 
GTCACTCATG ACACGAGACT TTTCTGTCTG ATGCTGCCTC CAAGTACTCA CCTTCAGGTG 
CCAGTTGGAC AACATGTTTA CCTCAAGCTC AGTGTCACAG GTGCAGAAAT AGTAAAGCCA 
TATACACCCG TGTCTGATTC CTTACTCTCA GATTTTAAAG AACCAGTCCT TTCCCCAAAT 
AAATACATCT GCTTTTTGAT CAAAATCTAT CCTGCTGGAC TCTTCACACC AGAGCTTGAT 
CGTCTTCAGA TTGGAGATTT TATTTCTGTA AGCGGACCTG AAGGCGATTT TAAAGTATCC 
AAACTCCAGG AAGTAGAAGA TCTCTTTCTA TTGGCAGCCG GAACAGGATT CACACCAATG 
GTCACTGTAC TGAATTATGC TTTGTCTCAC ATGTCCAGTC TCAGGAAGGT GAAGTTGATG 
TTCTTCAATA AAACAGAGGA CGATATAATT TGGAGATGTC AGCTAGAGAA GTTGGCATTA 
AGAGAAAAAA GATTTGATGT TGAATTTGTT CTCTCGGCAC CTTCTCCAGA ATGGAATGGC 
AAACAGGGAC ACATATCACG AGCTCTTCTC TCTGAATTTT TGCAAAGAAG TTCAGAAAAC 
TCTAGGGCCT TCCTTTGCAT CTGCGGACCA ACACCATTTA CTGACGAGGG GATAAGGTTG 
CTGCATGATC TTAACTTTTC TGATGATGAG ATTCACGGAT TCACCGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cyb5r4 ( XM_030244394.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100254.1 Mus musculus cytochrome b5 reductase 4 (Cyb5r4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu90189
Clone ID Related Accession (Same CDS sequence)	XM_030244394.1
Accession Version	XM_030244394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cytochrome b5 reductase 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAACG TCCCTTCCCA GGCTTTCCCG GCCCCCGGCT CCCAGCAGCG CGTCTCCTCC 
CAGGGGCGCA GCAAGGTACC TTTAAAACAA GGCAGGAGTC TTATGGATTG GATCCGACTG 
ACCAAGAGTG GAAAGGACTT AACAGGATTG AAGGGCGGGT TAATTGAAGT AACTGAAGAA 
GAGCTTAAGA AGCACAACAA GAAAGAAGAT TGTTGGATAT GCATAAGAGG GTTTGTTTAC 
AACGTCAGCC CTTATATGGA GTACCATCCG GGTGGGGAGG ATGAACTCAT GAGAGCCGCA 
GGAGCCGATG GAACAGATCT TTTTAATGAG GTTCATCGTT GGGTCAATTA TGAATCCATG 
CTGAAAGAAT GCCTGGTTGG CAGGATGGCT GTGAAGCCTG CTGTTCCAAA AGACTGTCAT 
GAAGGAAAGA GAGTCTTAAA TGGCATGCTT CCCAAGAGCC AAATGTCAGA CACACTGCCC 
AGGGATGTGA CAGACACACT GCCCAGGGAA GACCTCAGTT CTCCAAGCTA TGATTGGTTC 
CAAACAGAGT CTTCAGTCAC CATTGTTGTA TACACCAAAC AAAAGAATAT CAGTTTAGAC 
TCAGTAATCG TTGACCTCCA GGATGACTCC TTGAGAGCAG AAGCAGTCAT CAAGGATCAC 
TCTTACCTTG TACATGTTGG CTTGTACTAC AGACGGTGCC AGTTAATATC TAAGGAAGAT 
GTCACTCATG ACACGAGACT TTTCTGTCTG ATGCTGCCTC CAAGTACTCA CCTTCAGGTG 
CCAGTTGGAC AACATGTTTA CCTCAAGCTC AGTGTCACAG GAGATTTTAT TTCTGTAAGC 
GGACCTGAAG GCGATTTTAA AGTATCCAAA CTCCAGGAAG TAGAAGATCT CTTTCTATTG 
GCAGCCGGAA CAGGATTCAC ACCAATGGTC ACTGTACTGA ATTATGCTTT GTCTCACATG 
TCCAGTCTCA GGAAGGTGAA GTTGATGTTC TTCAATAAAA CAGAGGACGA TATAATTTGG 
AGATGTCAGC TAGAGAAGTT GGCATTAAGA GAAAAAAGAT TTGATGTTGA ATTTGTTCTC 
TCGGCACCTT CTCCAGAATG GAATGGCAAA CAGGGACACA TATCACGAGC TCTTCTCTCT 
GAATTTTTGC AAAGAAGTTC AGAAAACTCT AGGGCCTTCC TTTGCATCTG CGGACCAACA 
CCATTTACTG ACGAGGGGAT AAGGTTGCTG CATGATCTTA ACTTTTCTGA TGATGAGATT 
CACGGATTCA CCGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100254.1
CDS	172..1449
Translation	MLNVPSQAFPAPGSQQRVSSQGRSKVPLKQGRSLMDWIRLTKSGKDLTGLKGGLIEVTEEELKKHNKKEDCWICIRGFVYNVSPYMEYHPGGEDELMRAAGADGTDLFNEVHRWVNYESMLKECLVGRMAVKPAVPKDCHEGKRVLNGMLPKSQMSDTLPRDVTDTLPREDLSSPSYDWFQTESSVTIVVYTKQKNISLDSVIVDLQDDSLRAEAVIKDHSYLVHVGLYYRRCQLISKEDVTHDTRLFCLMLPPSTHLQVPVGQHVYLKLSVTGDFISVSGPEGDFKVSKLQEVEDLFLLAAGTGFTPMVTVLNYALSHMSSLRKVKLMFFNKTEDDIIWRCQLEKLALREKRFDVEFVLSAPSPEWNGKQGHISRALLSEFLQRSSENSRAFLCICGPTPFTDEGIRLLHDLNFSDDEIHGFTA

Target ORF information:

RefSeq Version	XM_030244394.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytochrome b5 reductase 4 (Cyb5r4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244394.1

ORF Insert Sequence:

ATGCTGAACG TCCCTTCCCA GGCTTTCCCG GCCCCCGGCT CCCAGCAGCG CGTCTCCTCC 
CAGGGGCGCA GCAAGGTACC TTTAAAACAA GGCAGGAGTC TTATGGATTG GATCCGACTG 
ACCAAGAGTG GAAAGGACTT AACAGGATTG AAGGGCGGGT TAATTGAAGT AACTGAAGAA 
GAGCTTAAGA AGCACAACAA GAAAGAAGAT TGTTGGATAT GCATAAGAGG GTTTGTTTAC 
AACGTCAGCC CTTATATGGA GTACCATCCG GGTGGGGAGG ATGAACTCAT GAGAGCCGCA 
GGAGCCGATG GAACAGATCT TTTTAATGAG GTTCATCGTT GGGTCAATTA TGAATCCATG 
CTGAAAGAAT GCCTGGTTGG CAGGATGGCT GTGAAGCCTG CTGTTCCAAA AGACTGTCAT 
GAAGGAAAGA GAGTCTTAAA TGGCATGCTT CCCAAGAGCC AAATGTCAGA CACACTGCCC 
AGGGATGTGA CAGACACACT GCCCAGGGAA GACCTCAGTT CTCCAAGCTA TGATTGGTTC 
CAAACAGAGT CTTCAGTCAC CATTGTTGTA TACACCAAAC AAAAGAATAT CAGTTTAGAC 
TCAGTAATCG TTGACCTCCA GGATGACTCC TTGAGAGCAG AAGCAGTCAT CAAGGATCAC 
TCTTACCTTG TACATGTTGG CTTGTACTAC AGACGGTGCC AGTTAATATC TAAGGAAGAT 
GTCACTCATG ACACGAGACT TTTCTGTCTG ATGCTGCCTC CAAGTACTCA CCTTCAGGTG 
CCAGTTGGAC AACATGTTTA CCTCAAGCTC AGTGTCACAG GAGATTTTAT TTCTGTAAGC 
GGACCTGAAG GCGATTTTAA AGTATCCAAA CTCCAGGAAG TAGAAGATCT CTTTCTATTG 
GCAGCCGGAA CAGGATTCAC ACCAATGGTC ACTGTACTGA ATTATGCTTT GTCTCACATG 
TCCAGTCTCA GGAAGGTGAA GTTGATGTTC TTCAATAAAA CAGAGGACGA TATAATTTGG 
AGATGTCAGC TAGAGAAGTT GGCATTAAGA GAAAAAAGAT TTGATGTTGA ATTTGTTCTC 
TCGGCACCTT CTCCAGAATG GAATGGCAAA CAGGGACACA TATCACGAGC TCTTCTCTCT 
GAATTTTTGC AAAGAAGTTC AGAAAACTCT AGGGCCTTCC TTTGCATCTG CGGACCAACA 
CCATTTACTG ACGAGGGGAT AAGGTTGCTG CATGATCTTA ACTTTTCTGA TGATGAGATT 
CACGGATTCA CCGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cyb5r4 ( XM_017313397.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168886.1 Mus musculus cytochrome b5 reductase 4 (Cyb5r4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu82830
Clone ID Related Accession (Same CDS sequence)	XM_017313397.1
Accession Version	XM_017313397.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cytochrome b5 reductase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAACG TCCCTTCCCA GGCTTTCCCG GCCCCCGGCT CCCAGCAGCG CGTCTCCTCC 
CAGGGGCGCA GCAAGGTACC TTTAAAACAA GGCAGGAGTC TTATGGATTG GATCCGACTG 
ACCAAGAGTG GAAAGGACTT AACAGGATTG AAGGGCGGGT TAATTGAAGT AACTGAAGAA 
GAGCTTAAGA AGCACAACAA GAAAGAAGAT TGTTGGATAT GCATAAGAGG GTTTGTTTAC 
AACGTCAGCC CTTATATGGA GTACCATCCG GGTGGGGAGG ATGAACTCAT GAGAGCCGCA 
GGAGCCGATG GAACAGATCT TTTTAATGAG GTTCATCGTT GGGTCAATTA TGAATCCATG 
CTGAAAGAAT GCCTGGTTGG CAGGATGGCT GTGAAGCCTG CTGTTCCAAA AGACTGTCAT 
GAAGGAAAGA GAGTCTTAAA TGGCATGCTT CCCAAGAGCC AAATGTCAGA CACACTGCCC 
AGGGATGTGA CAGACACACT GCCCAGGGAA GACCTCAGTT CTCCAAGCTA TGATTGGTTC 
CAAACAGAGT CTTCAGTCAC CATTGTTGTA TACACCAAAC AAAAGAATAT CAGTTTAGAC 
TCAGTAATCG TTGACCTCCA GGATGACTCC TTGAGAGCAG AAGCAGTCAT CAAGGATCAC 
TCTTACCTTG TACATGTTGT ACGGGTTATT GAAAATGTTG GAAAAATAGA GATTGTCCTA 
CAAAAGAAAG AAAGTGTTTC TTGGCAATGT CTTGGTGATC ACCTGGAGAA ACATGATTCT 
TTCATTCCAA AGAAAGATAC AGGCTTGTAC TACAGACGGT GCCAGTTAAT ATCTAAGGAA 
GATGTCACTC ATGACACGAG ACTTTTCTGT CTGATGCTGC CTCCAAGTAC TCACCTTCAG 
GTGCCAGTTG GACAACATGT TTACCTCAAG CTCAGTGTCA CAGGTGCAGA AATAGTAAAG 
CCATATACAC CCGTGTCTGA TTCCTTACTC TCAGATTTTA AAGAACCAGT CCTTTCCCCA 
AATAAATACA TCTGCTTTTT GATCAAAATC TATCCTGCTG GACTCTTCAC ACCAGAGCTT 
GATCGTCTTC AGATTGGAGA TTTTATTTCT GTAAGCGGAC CTGAAGGCGA TTTTAAAGTA 
TCCAAACTCC AGGAAGTAGA AGATCTCTTT CTATTGGCAG CCGGAACAGG ATTCACACCA 
ATGGTCACTG TACTGAATTA TGCTTTGTCT CACATGTCCA GTCTCAGGAA GGTGAAGTTG 
ATGTTCTTCA ATAAAACAGA GGACGATATA ATTTGGAGAT GTCAGCTAGA GAAGTTGGCA 
TTAAGAGAAA AAAGATTTGA TGTTGAATTT GTTCTCTCGG CACCTTCTCC AGAATGGAAT 
GGCAAACAGG GACACATATC ACGAGCTCTT CTCTCTGAAT TTTTGCAAAG AAGTTCAGAA 
AACTCTAGGG CCTTCCTTTG CATCTGCGGA CCAACACCAT TTACTGACGA GGGGATAAGG 
TTGCTGCATG ATCTTAACTT TTCTGATGAT GAGATTCACG GATTCACCGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168886.1
CDS	172..1725
Translation	MLNVPSQAFPAPGSQQRVSSQGRSKVPLKQGRSLMDWIRLTKSGKDLTGLKGGLIEVTEEELKKHNKKEDCWICIRGFVYNVSPYMEYHPGGEDELMRAAGADGTDLFNEVHRWVNYESMLKECLVGRMAVKPAVPKDCHEGKRVLNGMLPKSQMSDTLPRDVTDTLPREDLSSPSYDWFQTESSVTIVVYTKQKNISLDSVIVDLQDDSLRAEAVIKDHSYLVHVVRVIENVGKIEIVLQKKESVSWQCLGDHLEKHDSFIPKKDTGLYYRRCQLISKEDVTHDTRLFCLMLPPSTHLQVPVGQHVYLKLSVTGAEIVKPYTPVSDSLLSDFKEPVLSPNKYICFLIKIYPAGLFTPELDRLQIGDFISVSGPEGDFKVSKLQEVEDLFLLAAGTGFTPMVTVLNYALSHMSSLRKVKLMFFNKTEDDIIWRCQLEKLALREKRFDVEFVLSAPSPEWNGKQGHISRALLSEFLQRSSENSRAFLCICGPTPFTDEGIRLLHDLNFSDDEIHGFTA

Target ORF information:

RefSeq Version	XM_017313397.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytochrome b5 reductase 4 (Cyb5r4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313397.1

ORF Insert Sequence:

ATGCTGAACG TCCCTTCCCA GGCTTTCCCG GCCCCCGGCT CCCAGCAGCG CGTCTCCTCC 
CAGGGGCGCA GCAAGGTACC TTTAAAACAA GGCAGGAGTC TTATGGATTG GATCCGACTG 
ACCAAGAGTG GAAAGGACTT AACAGGATTG AAGGGCGGGT TAATTGAAGT AACTGAAGAA 
GAGCTTAAGA AGCACAACAA GAAAGAAGAT TGTTGGATAT GCATAAGAGG GTTTGTTTAC 
AACGTCAGCC CTTATATGGA GTACCATCCG GGTGGGGAGG ATGAACTCAT GAGAGCCGCA 
GGAGCCGATG GAACAGATCT TTTTAATGAG GTTCATCGTT GGGTCAATTA TGAATCCATG 
CTGAAAGAAT GCCTGGTTGG CAGGATGGCT GTGAAGCCTG CTGTTCCAAA AGACTGTCAT 
GAAGGAAAGA GAGTCTTAAA TGGCATGCTT CCCAAGAGCC AAATGTCAGA CACACTGCCC 
AGGGATGTGA CAGACACACT GCCCAGGGAA GACCTCAGTT CTCCAAGCTA TGATTGGTTC 
CAAACAGAGT CTTCAGTCAC CATTGTTGTA TACACCAAAC AAAAGAATAT CAGTTTAGAC 
TCAGTAATCG TTGACCTCCA GGATGACTCC TTGAGAGCAG AAGCAGTCAT CAAGGATCAC 
TCTTACCTTG TACATGTTGT ACGGGTTATT GAAAATGTTG GAAAAATAGA GATTGTCCTA 
CAAAAGAAAG AAAGTGTTTC TTGGCAATGT CTTGGTGATC ACCTGGAGAA ACATGATTCT 
TTCATTCCAA AGAAAGATAC AGGCTTGTAC TACAGACGGT GCCAGTTAAT ATCTAAGGAA 
GATGTCACTC ATGACACGAG ACTTTTCTGT CTGATGCTGC CTCCAAGTAC TCACCTTCAG 
GTGCCAGTTG GACAACATGT TTACCTCAAG CTCAGTGTCA CAGGTGCAGA AATAGTAAAG 
CCATATACAC CCGTGTCTGA TTCCTTACTC TCAGATTTTA AAGAACCAGT CCTTTCCCCA 
AATAAATACA TCTGCTTTTT GATCAAAATC TATCCTGCTG GACTCTTCAC ACCAGAGCTT 
GATCGTCTTC AGATTGGAGA TTTTATTTCT GTAAGCGGAC CTGAAGGCGA TTTTAAAGTA 
TCCAAACTCC AGGAAGTAGA AGATCTCTTT CTATTGGCAG CCGGAACAGG ATTCACACCA 
ATGGTCACTG TACTGAATTA TGCTTTGTCT CACATGTCCA GTCTCAGGAA GGTGAAGTTG 
ATGTTCTTCA ATAAAACAGA GGACGATATA ATTTGGAGAT GTCAGCTAGA GAAGTTGGCA 
TTAAGAGAAA AAAGATTTGA TGTTGAATTT GTTCTCTCGG CACCTTCTCC AGAATGGAAT 
GGCAAACAGG GACACATATC ACGAGCTCTT CTCTCTGAAT TTTTGCAAAG AAGTTCAGAA 
AACTCTAGGG CCTTCCTTTG CATCTGCGGA CCAACACCAT TTACTGACGA GGGGATAAGG 
TTGCTGCATG ATCTTAACTT TTCTGATGAT GAGATTCACG GATTCACCGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NCB5OR Deficiency in the Cerebellum and Midbrain Leads to Dehydration and Alterations in Thirst Response, Fasted Feeding Behavior, and Voluntary Exercise in Mice.
Cerebellum (London, England)(2017 Sep)
Stroh MA,Winter MK,McCarson KE,Thyfault JP,Zhu H

Loss of NCB5OR in the cerebellum disturbs iron pathways, potentiates behavioral abnormalities, and exacerbates harmaline-induced tremor in mice.
Metabolic brain disease31(4)951-64(2016 Aug)
Stroh MA,Winter MK,Swerdlow RH,McCarson KE,Zhu H

Cytochrome b5 and epoxide hydrolase contribute to benzo[a]pyrene-DNA adduct formation catalyzed by cytochrome P450 1A1 under low NADPH:P450 oxidoreductase conditions.
Toxicology3181-12(2014 Apr)
Stiborov? M,Moserov? M,?ern? V,Indra R,Dra??nsk? M,?ulc M,Henderson CJ,Wolf CR,Schmeiser HH,Phillips DH,Frei E,Arlt VM

Development of diabetes in lean Ncb5or-null mice is associated with manifestations of endoplasmic reticulum and oxidative stress in beta cells.
Biochimica et biophysica acta1812(11)1532-41(2011 Nov)
Wang W,Guo Y,Xu M,Huang HH,Novikova L,Larade K,Jiang ZG,Thayer TC,Frontera JR,Aires D,Ding H,Turk J,Mathews CE,Bunn HF,Stehno-Bittel L,Zhu H

Ncb5or deficiency increases fatty acid catabolism and oxidative stress.
The Journal of biological chemistry286(13)11141-54(2011 Apr)
Xu M,Wang W,Frontera JR,Neely MC,Lu J,Aires D,Hsu FF,Turk J,Swerdlow RH,Carlson SE,Zhu H

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Cyb5r4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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