Rttn cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rttn
Entrez Gene ID	246102
Full Name	rotatin
Synonyms	4921538A15Rik,AI666264,C530033I08Rik,D230040K24
General protein information	Preferred Names rotatin Names rotatin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18\|18 E4

mRNA and Protein(s)

mRNA	Protein	Name
XM_006526477.3	XP_006526540.1	rotatin isoform X1
XM_030250463.1	XP_030106323.1	rotatin isoform X1
XM_011247080.2	XP_011245382.1	rotatin isoform X2
NM_175542.3	NP_780751.2	rotatin
XM_011247080.3	XP_011245382.1	rotatin isoform X2

Pathways from BioSystems

Homologies

	RTTN	XP_001095442.1
Canis lupus familiaris (dog)	RTTN	XP_541060.2
Bos taurus (cattle)	RTTN	NP_001193136.1
Mus musculus (house mouse)	Rttn	NP_780751.2
Homo sapiens (human)	RTTN	NP_775901.3
Danio rerio (zebrafish)	rttn	XP_001920443.3
Xenopus tropicalis (tropical clawed frog)	rttn	XP_002939666.2
Gallus gallus (chicken)	RTTN	NP_001243472.1
Pan troglodytes (chimpanzee)	RTTN	XP_512171.3
Rattus norvegicus (Norway rat)	Rttn	NP_001163907.1

Gene Ontology
Bibliography

Related articles in PubMed

Nt mutation causing laterality defects associated with deletion of rotatin.
Chatterjee B, Richards K, Bucan M, Lo C
Mammalian genome : official journal of the International Mammalian Genome Society18(5)310-5(2007 May)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

Rotatin is a novel gene required for axial rotation and left-right specification in mouse embryos.
Faisst AM, Alvarez-Bolado G, Treichel D, Gruss P
Mechanisms of development113(1)15-28(2002 Apr)

Gene trap expression and mutational analysis for genes involved in the development of the mammalian nervous system.
Stoykova A, Chowdhury K, Bonaldo P, Torres M, Gruss P
Developmental dynamics : an official publication of the American Association of Anatomists212(2)198-213(1998 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

No turning (nt), a recessive lethal mutation causing left-right and axial patterning defects is likely elicited by deficiency of Rttn on chromosome 18.
Title: Nt mutation causing laterality defects associated with deletion of rotatin.

cloning and characterization of rotatin
Title: Rotatin is a novel gene required for axial rotation and left-right specification in mouse embryos.

The following Rttn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rttn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu54948	XM_030250463.1 Latest version!	Mus musculus rotatin (Rttn), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu74252	XM_011247080.3 Latest version!	Mus musculus rotatin (Rttn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu54948	XM_006526477.3 Latest version!	Mus musculus rotatin (Rttn), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu05549	NM_175542.3 Latest version!	Mus musculus rotatin (Rttn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1637.30 $2339.00
View Old Accession Versions >>
OMu74252	XM_011247080.2	Mus musculus rotatin (Rttn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rttn ( XM_030250463.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106323.1 Mus musculus rotatin (Rttn), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu54948
Clone ID Related Accession (Same CDS sequence)	XM_006526477.3 , XM_030250463.1
Accession Version	XM_030250463.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rotatin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAATAA GAGTTCTGGA ACTGCTTGCA GAGGACATGC TGCTCATTGG ACAAGCAATT 
TCATCAGAAA TCTGGGATGA TAACAGCCTT TTTGCTATCA ATGTGAAAGA GAAACTGCTC 
CAAGTGTTGG GGTCCCTTGG AGAAACCATG TGCTACCGTA AAACCAGTTT TAGCATGGAG 
CAAGCCGAGG CTGTACTCGT GCACCACAGA ATGGCCTTTG TCAGCATCTC ACTGTTCACA 
GTTCGACTGT TGCAACTTCT TCTCCCTGTT GAAAAGGCGA GTGAGTTTTT GCCAGAGTCC 
ATGTCAGCAG CATTATTTTT TGTCTCCTTG GACATGCCTA TTTCTTTGGA ATATCCAGAG 
ATTCATGACA CAGTTGTGGC TTATTTGGAA CAGCTGGATT CTGAAAATTA CAGCATTTAT 
AAGCGAACTG CAGAGGCTGT TTATTCAATT GAATGCACCT GTAACTTCCT ATCTGATGTG 
GGGAAAGAGG GAGAAAAGAA TCTTCTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTCTCCCCTA ATAAAGGAAA TCATCTGCAT CTGTTCAAAG 
ATCTGGAAAT CTGCACAGGC TAGTCCATTA CTCCAGGGAG AGAGTCAGAA GGTGTTTCTC 
CATATGCTGT CTCACCCATT GCCACAGGTG AAGGTGGAGA CTTACCAGTG CTGCCTGGAG 
ATTGTAAAGG AATGTTTAGG TGTCCAAAAT GTCACTAAAC CTGTGTCTTC ACTTTGTAGT 
GGAATTCATT TTCTGCTTCA TCCAAAAGTT CTGTATGAAA TCTCTGCATT TGGTATTCAA 
GAATCCAAAA ATGAGGTGAA TGCTGCTGCC AAGGCCATTC TGCTGTATCT GCTTCAGGGA 
CGATTGATGA TGACAGCTCT GACCTGGAAC AAGTTTATCG AATCTCTCGC TCCTGTCATT 
CCAGTGCTAC AGGGCTACGC TGATACAGAA GATCCCTTAG GCAACTGCAT TCTCCTTCTA 
AGCAAAGTGA GCTCTGAGGC AGGAGAAGGC GCTCTCCCCT GCACTGCCCG GCTGAAGTCT 
GTGCTACGGC TTTTGCTAGT AAAAAAACCT TCGGTTCGTT CACTAGCATT AAAACTTTTA 
GCATTTCATC TTACCAGTGA AGAAGGGGCT GATAAAAAGC GCCCGTTCAT AGATGCTGGC 
GTTCTCTCCA GAGTTACTAA TCTGTTTATT GTGAAAAAAC CTATTGACCT TAAGTTGGAT 
GACAGAAGAG AACTAGTTAT TAAGTTGGAG ACTGTTAAAA AGGTGTGTGA CATATTCACC 
TCAGACGATG TTGATCTTGT TTTAAGAAAG TCAGCTGCAG AGCAACTAGC TGTGATAATG 
CAAGATATTA AAATGCATAC AGTGGTGAAA AAGTTATGCT TAGTTGAGAA GATTATTGGA 
CATTTAAATG AATTTGTGGG TCAAGATGGT GAGGTTATAG AATGCTTGAT TCAGCCATGC 
CTCACACTCC TGAGGAAGGT TTTGTACGCT GACCCGGTTA TCCGTGTTTC CTTGTCACAG 
CAGGCCTCTC TCTTGACCCT ATTACTCAGA GTTTCCTTGA TATTTCATGA AGATTGTACT 
GTGGTGGCTG AAGTCGGGGC ATTGTTTTGT CTTCTATTGT TCGATGAAGT ATCAAGAATG 
GATACATGGT CTGATACTCT TTCTGATAAG CCTTCTTTGT CATCAGTCTT CAGTTTGCCG 
GTTTCAGTCT TTAGAAGGTA TCATCTACCA GTTCATGTGA CTGGCCACCA TGCTGTGAGT 
CCTTACTCAA TAGTTTTGCC ATTACCTACT GAATGTTTGA CCCTGAAGCC GGTGTCAGAT 
ATGCTGAGAA TAGCTTGGAA CCTGGCATGG TATCATGGGA GTGATAATCT ATTGAAACAA 
ATGACCTCTG AAGCTAAAAT TCAGGAATTT TTAGATACAC TGCAGCTGTC CACAGAAGAT 
ATCCTTGCAC TGAAGATCAC ATACACGGAT AGTGGGTTGA AGGACTGCCT TCAGTCCATC 
ATTCAGGCTG GAGGCCACAG GGATGTGAGG GCTGCTATCA CCAGGATGAG TTTTTATCTT 
TTGAATGACA AACTGTCTTT GAAGGATGAT ATTGGTCCAT GTGGCGTTAC TTTAAAATCC 
TTGGCTTGGC ACACTGTGCT GAACAGGTTT TTACAAGTAC TTCCTGCTTG CACTGAAGAT 
GAGAAACTGC TAATAGATAT CATACATTTT TTGAATAAGT TAATAAAGGA ACAAAGAAAA 
AACCCATCAA TTGAACTTTT AAACTGGATT CTGGAATTAC TTCTTAGGCA TAATCCAAGT 
CCACTACTAG ACCTGCTGGT TCTGACAGAT TCACATGCAG GAGAAGAAAC AGATGACGTC 
CGGACTGCTG TCAGACAGCA ACTCCAGAAA GAACTGATCA GTCTTCTCAA TTCCTTGTTA 
CTGGTTTTTA TGACAGTCTC TGACAGGAAA TGCTTGGAAC TGTTTTACGT TTTCCAAACA 
CAGCTGTCTC TGAAGCTACT CCAGTGCCTC AGAGTCACAG ATGCTCCCCA TTTCTATGGC 
TTACCTTCAT TGGAGAGGAC CTTAGCAGGG ATGGCTCACC TCACTGCCTT CCCTGGGTGG 
AGTGCACACT CTCCTCTTTC AAAGCCACTT GAGATTTGTG GGAAGTACTT GTCCGGTCTC 
CTGGAGGTCA TTACTTCCTT TTATGTTGAA CGGGGAGGAA ATGCTATGTC CTTCATGGGG 
AAAGGCGTTA CAAAGAGCAC AGGTCTTTGC TTACTTCACT TATCCCATGA GATGATAGTC 
CAGGCACCAG GCTCGGAGTG GATGCCGCTT TGGTTCTTGC CTTTGGGTAG TCACAGTGAA 
GAACATGCCC CTACTCAGCA AGGATTGGCC TGGCTAATTC CCTTATGGGT TGATAGAGAC 
CCAGAGGTGA GATTCACTTC ACTGGGGTTG GGATCAGCAC TGACTACTAT TGAAGCTGGC 
TGTGTGGCCT TGGCAAACAG TTGTCAGAAT ATTTCTGGTG GGCTCTGGGG AACCGTGGTG 
AACATTCTTC TGGATCAATC AGAATGCAGT CTGGTGCGCC GGGAGGCTGC ATTTATTCTT 
CAGAATCTCC TTGTAATTCC AATGCCTTCA GAAATTATAA AGGATTATAC TTGGCAGGGC 
CCCTGTGTCC ATGATGAGGA TTCTGGCCTG TCACTTTCAG GAAAACCTGC CCTTCAGGCT 
CTTTTATATC ACTGTCGTTT CTATGAACAT TTGACTCACA TGGTGAAACA TTGTTATGTA 
GGAAGATATA TCTTTGATTT TAATTTTTCT GCTTTTACTG AAACTTCAGA ATACAGTGAT 
TTAAATGGTT TAGACGACTC ATTCAAGTTT TGGAGGGCCC CTTCTGGGAC ATCTACTCAG 
GATCATGAGC CAAGCTCTCT TTCCACCTCA GAAACAATGG TGGCTGCCTC GGTGGTCAGT 
TCTGAGATTC AGCCCCTGCT GCCGTCAACA CTTCTTCCTG AAACTGCCCT TGACCAGTTT 
GTGACGCAAG GTCAACGTGA AATGAGACCA GTGCGACCTC GTGACTCATT GCTGTCTACT 
TCCCTGAACA GGCAGTATGT GTTTGTTACT CCGTCTCTGC TGTCAGCAGT CTGTAGCCTT 
TTAGACAACC TCTTGGCAGT TACTCCAAGA GATGCTGCAA ATGCTTTTCA ACAAGCTCAT 
CTCATAGAAC TCCTCTGTAG CACGGTGGAT GCTTCCCTCA TAGAAATGTG CATCCAAGAA 
CTCAAGACCC CACTGCCTTT ACCATCTGCT GTTGAACATA TTCAGGCTCA GGCTTCCTTT 
TTCTTGGAAT ACCTATCCTC TTTGTCCCAG CTCTTGAAGT CCTGTCTGTT GGTAGAGCCT 
AAACTTGTGA CACAAGATGA GCTCTTGAAA CCTCTTATCA CTAATGTCAT CCGAGTTCTC 
ATTGTGTGTC CCAAAGATGT ATTAGATGTA CAGTTAGTGT TGGCTTTTTA CCGCACATGG 
ACGCATTTAT TTGACCTTCT GGCCACACTA CTGAGGAAAG CTGGTGCTGT CTCCCTTGTT 
CCTCTTACCT CAGCTCTTGC CAAGCATTGG GAGGCAGTGA CTGATACACT GTGTAGATGT 
GTGGGCCTCT CATTCAAGTG CCCTGCCCTG ACTATTGCCA GCCTCCAGTT CCTGTCTGTT 
CTCTTGGCTG AGGAAGAAAA GAGGCGTGTC CAGGACAAGG ACAAAACAAA TGAGGGCCAG 
GCCCCAACAG TGGCCTTACT TCTTGATGGA ACTCAGGGAA GCCTTAGCTC TTCAGAGCGA 
CTTAATGAAA CAATTCTTCA GTGCTATGAA GGAATATCCC CCAAAGATGT CCTGAAAAGA 
GTTGCTGCAA ATGCCCTGTT GTCACTTCTG GCTGTTAGCA GAAGAGCGCA GAGGCATGCT 
TTGAGATCTG ATCTCATAGA AAACTGTATG GAGCAGATGA AGCACATCAA TGCACAACTG 
AACTTAGATT TGCTGAGACC TGGGAAAGCA GTACTGAAAA AAAAGGAGGA TGGCTTCATT 
AAAGAATTAA GCATCACCAT GCAGCTCCTA AGAAACTGCC TTTATCAAAA TGAAGAATGT 
AAAGTGGCAG CTCTTGAAGC ACGTCTTGTG CCTGTATTGC ACTCTCTCTG GCCTTGGCTT 
TTGATGGAAG ATTCATTGAT GCAAATTGCC TTGCAGTTGC TTTGTGTCTA TACTGCAAAT 
TTTCCAGCTG GCTGCAGTTC CCTTTGTTGG TCAAATTATG GACAGTGTAT TCAAACTGCA 
CACAGAGGCA CCCCCAGTAG CTCCCTGATA CTGTGTATCC TGAAACTGGC TTCTCAGATG 
CCTGCAGAGA ACACCACCAT TCAGCAGTTG GTTTTTATGT TTCTTTCAAA CTTGGCCTTA 
TCTCATGACT GCAAGGGAGT AATTCAAAAG AGCAACTTTC TACAGCACTT CTTGTCCCTG 
ACATTGCCTA AAGGAGGAAA CCAACATCTC AGCACTGTGG CCATTCTGTG GTTGAAGTTG 
CTCCTGAATA TGTCATTTGG AGAAGATGGA CAGCAAATGA TCCTGAGGCT AGATGGCTGC 
TTAGACTTAC TCATAGAGAT GAGCAAGTAC AAGCACAAGA GCAGCCCTCA CATGCCTCTG 
CTGATCTTTC ATAACATTTG CTTCAGTCCT GCTAATAAGC CTAAAATCCT GGCTAATGAA 
AAAGTTGTTA CTCTTCTGGC TGCCTGTTTA GAAAGTGAGA ATCAAACTGC CCAGAGGATT 
GGAGCAGCTT CACTTTGGGC TCTAACTTAC AATTACCAAA AGGCAAAAGC AACTTTGAAA 
AATCCATCAA TAAAAAGAAA AGTAGATGAA GCATATTCCA TAGCAAAGAG AACTCTCTCA 
AACTCAGAAG AAAACCCTCT GAATTCCTAC TATTTGAAAT GCCTTGAAAA TCTAGTACAA 
CTGCTTAACT ATTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106323.1
CDS	1566..7043
Translation	MIIRVLELLAEDMLLIGQAISSEIWDDNSLFAINVKEKLLQVLGSLGETMCYRKTSFSMEQAEAVLVHHRMAFVSISLFTVRLLQLLLPVEKASEFLPESMSAALFFVSLDMPISLEYPEIHDTVVAYLEQLDSENYSIYKRTAEAVYSIECTCNFLSDVGKEGEKNLLELVELADQALRSFSYHQHSPLIKEIICICSKIWKSAQASPLLQGESQKVFLHMLSHPLPQVKVETYQCCLEIVKECLGVQNVTKPVSSLCSGIHFLLHPKVLYEISAFGIQESKNEVNAAAKAILLYLLQGRLMMTALTWNKFIESLAPVIPVLQGYADTEDPLGNCILLLSKVSSEAGEGALPCTARLKSVLRLLLVKKPSVRSLALKLLAFHLTSEEGADKKRPFIDAGVLSRVTNLFIVKKPIDLKLDDRRELVIKLETVKKVCDIFTSDDVDLVLRKSAAEQLAVIMQDIKMHTVVKKLCLVEKIIGHLNEFVGQDGEVIECLIQPCLTLLRKVLYADPVIRVSLSQQASLLTLLLRVSLIFHEDCTVVAEVGALFCLLLFDEVSRMDTWSDTLSDKPSLSSVFSLPVSVFRRYHLPVHVTGHHAVSPYSIVLPLPTECLTLKPVSDMLRIAWNLAWYHGSDNLLKQMTSEAKIQEFLDTLQLSTEDILALKITYTDSGLKDCLQSIIQAGGHRDVRAAITRMSFYLLNDKLSLKDDIGPCGVTLKSLAWHTVLNRFLQVLPACTEDEKLLIDIIHFLNKLIKEQRKNPSIELLNWILELLLRHNPSPLLDLLVLTDSHAGEETDDVRTAVRQQLQKELISLLNSLLLVFMTVSDRKCLELFYVFQTQLSLKLLQCLRVTDAPHFYGLPSLERTLAGMAHLTAFPGWSAHSPLSKPLEICGKYLSGLLEVITSFYVERGGNAMSFMGKGVTKSTGLCLLHLSHEMIVQAPGSEWMPLWFLPLGSHSEEHAPTQQGLAWLIPLWVDRDPEVRFTSLGLGSALTTIEAGCVALANSCQNISGGLWGTVVNILLDQSECSLVRREAAFILQNLLVIPMPSEIIKDYTWQGPCVHDEDSGLSLSGKPALQALLYHCRFYEHLTHMVKHCYVGRYIFDFNFSAFTETSEYSDLNGLDDSFKFWRAPSGTSTQDHEPSSLSTSETMVAASVVSSEIQPLLPSTLLPETALDQFVTQGQREMRPVRPRDSLLSTSLNRQYVFVTPSLLSAVCSLLDNLLAVTPRDAANAFQQAHLIELLCSTVDASLIEMCIQELKTPLPLPSAVEHIQAQASFFLEYLSSLSQLLKSCLLVEPKLVTQDELLKPLITNVIRVLIVCPKDVLDVQLVLAFYRTWTHLFDLLATLLRKAGAVSLVPLTSALAKHWEAVTDTLCRCVGLSFKCPALTIASLQFLSVLLAEEEKRRVQDKDKTNEGQAPTVALLLDGTQGSLSSSERLNETILQCYEGISPKDVLKRVAANALLSLLAVSRRAQRHALRSDLIENCMEQMKHINAQLNLDLLRPGKAVLKKKEDGFIKELSITMQLLRNCLYQNEECKVAALEARLVPVLHSLWPWLLMEDSLMQIALQLLCVYTANFPAGCSSLCWSNYGQCIQTAHRGTPSSSLILCILKLASQMPAENTTIQQLVFMFLSNLALSHDCKGVIQKSNFLQHFLSLTLPKGGNQHLSTVAILWLKLLLNMSFGEDGQQMILRLDGCLDLLIEMSKYKHKSSPHMPLLIFHNICFSPANKPKILANEKVVTLLAACLESENQTAQRIGAASLWALTYNYQKAKATLKNPSIKRKVDEAYSIAKRTLSNSEENPLNSYYLKCLENLVQLLNYS

Target ORF information:

RefSeq Version	XM_030250463.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus rotatin (Rttn), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250463.1

ORF Insert Sequence:

ATGATAATAA GAGTTCTGGA ACTGCTTGCA GAGGACATGC TGCTCATTGG ACAAGCAATT 
TCATCAGAAA TCTGGGATGA TAACAGCCTT TTTGCTATCA ATGTGAAAGA GAAACTGCTC 
CAAGTGTTGG GGTCCCTTGG AGAAACCATG TGCTACCGTA AAACCAGTTT TAGCATGGAG 
CAAGCCGAGG CTGTACTCGT GCACCACAGA ATGGCCTTTG TCAGCATCTC ACTGTTCACA 
GTTCGACTGT TGCAACTTCT TCTCCCTGTT GAAAAGGCGA GTGAGTTTTT GCCAGAGTCC 
ATGTCAGCAG CATTATTTTT TGTCTCCTTG GACATGCCTA TTTCTTTGGA ATATCCAGAG 
ATTCATGACA CAGTTGTGGC TTATTTGGAA CAGCTGGATT CTGAAAATTA CAGCATTTAT 
AAGCGAACTG CAGAGGCTGT TTATTCAATT GAATGCACCT GTAACTTCCT ATCTGATGTG 
GGGAAAGAGG GAGAAAAGAA TCTTCTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTCTCCCCTA ATAAAGGAAA TCATCTGCAT CTGTTCAAAG 
ATCTGGAAAT CTGCACAGGC TAGTCCATTA CTCCAGGGAG AGAGTCAGAA GGTGTTTCTC 
CATATGCTGT CTCACCCATT GCCACAGGTG AAGGTGGAGA CTTACCAGTG CTGCCTGGAG 
ATTGTAAAGG AATGTTTAGG TGTCCAAAAT GTCACTAAAC CTGTGTCTTC ACTTTGTAGT 
GGAATTCATT TTCTGCTTCA TCCAAAAGTT CTGTATGAAA TCTCTGCATT TGGTATTCAA 
GAATCCAAAA ATGAGGTGAA TGCTGCTGCC AAGGCCATTC TGCTGTATCT GCTTCAGGGA 
CGATTGATGA TGACAGCTCT GACCTGGAAC AAGTTTATCG AATCTCTCGC TCCTGTCATT 
CCAGTGCTAC AGGGCTACGC TGATACAGAA GATCCCTTAG GCAACTGCAT TCTCCTTCTA 
AGCAAAGTGA GCTCTGAGGC AGGAGAAGGC GCTCTCCCCT GCACTGCCCG GCTGAAGTCT 
GTGCTACGGC TTTTGCTAGT AAAAAAACCT TCGGTTCGTT CACTAGCATT AAAACTTTTA 
GCATTTCATC TTACCAGTGA AGAAGGGGCT GATAAAAAGC GCCCGTTCAT AGATGCTGGC 
GTTCTCTCCA GAGTTACTAA TCTGTTTATT GTGAAAAAAC CTATTGACCT TAAGTTGGAT 
GACAGAAGAG AACTAGTTAT TAAGTTGGAG ACTGTTAAAA AGGTGTGTGA CATATTCACC 
TCAGACGATG TTGATCTTGT TTTAAGAAAG TCAGCTGCAG AGCAACTAGC TGTGATAATG 
CAAGATATTA AAATGCATAC AGTGGTGAAA AAGTTATGCT TAGTTGAGAA GATTATTGGA 
CATTTAAATG AATTTGTGGG TCAAGATGGT GAGGTTATAG AATGCTTGAT TCAGCCATGC 
CTCACACTCC TGAGGAAGGT TTTGTACGCT GACCCGGTTA TCCGTGTTTC CTTGTCACAG 
CAGGCCTCTC TCTTGACCCT ATTACTCAGA GTTTCCTTGA TATTTCATGA AGATTGTACT 
GTGGTGGCTG AAGTCGGGGC ATTGTTTTGT CTTCTATTGT TCGATGAAGT ATCAAGAATG 
GATACATGGT CTGATACTCT TTCTGATAAG CCTTCTTTGT CATCAGTCTT CAGTTTGCCG 
GTTTCAGTCT TTAGAAGGTA TCATCTACCA GTTCATGTGA CTGGCCACCA TGCTGTGAGT 
CCTTACTCAA TAGTTTTGCC ATTACCTACT GAATGTTTGA CCCTGAAGCC GGTGTCAGAT 
ATGCTGAGAA TAGCTTGGAA CCTGGCATGG TATCATGGGA GTGATAATCT ATTGAAACAA 
ATGACCTCTG AAGCTAAAAT TCAGGAATTT TTAGATACAC TGCAGCTGTC CACAGAAGAT 
ATCCTTGCAC TGAAGATCAC ATACACGGAT AGTGGGTTGA AGGACTGCCT TCAGTCCATC 
ATTCAGGCTG GAGGCCACAG GGATGTGAGG GCTGCTATCA CCAGGATGAG TTTTTATCTT 
TTGAATGACA AACTGTCTTT GAAGGATGAT ATTGGTCCAT GTGGCGTTAC TTTAAAATCC 
TTGGCTTGGC ACACTGTGCT GAACAGGTTT TTACAAGTAC TTCCTGCTTG CACTGAAGAT 
GAGAAACTGC TAATAGATAT CATACATTTT TTGAATAAGT TAATAAAGGA ACAAAGAAAA 
AACCCATCAA TTGAACTTTT AAACTGGATT CTGGAATTAC TTCTTAGGCA TAATCCAAGT 
CCACTACTAG ACCTGCTGGT TCTGACAGAT TCACATGCAG GAGAAGAAAC AGATGACGTC 
CGGACTGCTG TCAGACAGCA ACTCCAGAAA GAACTGATCA GTCTTCTCAA TTCCTTGTTA 
CTGGTTTTTA TGACAGTCTC TGACAGGAAA TGCTTGGAAC TGTTTTACGT TTTCCAAACA 
CAGCTGTCTC TGAAGCTACT CCAGTGCCTC AGAGTCACAG ATGCTCCCCA TTTCTATGGC 
TTACCTTCAT TGGAGAGGAC CTTAGCAGGG ATGGCTCACC TCACTGCCTT CCCTGGGTGG 
AGTGCACACT CTCCTCTTTC AAAGCCACTT GAGATTTGTG GGAAGTACTT GTCCGGTCTC 
CTGGAGGTCA TTACTTCCTT TTATGTTGAA CGGGGAGGAA ATGCTATGTC CTTCATGGGG 
AAAGGCGTTA CAAAGAGCAC AGGTCTTTGC TTACTTCACT TATCCCATGA GATGATAGTC 
CAGGCACCAG GCTCGGAGTG GATGCCGCTT TGGTTCTTGC CTTTGGGTAG TCACAGTGAA 
GAACATGCCC CTACTCAGCA AGGATTGGCC TGGCTAATTC CCTTATGGGT TGATAGAGAC 
CCAGAGGTGA GATTCACTTC ACTGGGGTTG GGATCAGCAC TGACTACTAT TGAAGCTGGC 
TGTGTGGCCT TGGCAAACAG TTGTCAGAAT ATTTCTGGTG GGCTCTGGGG AACCGTGGTG 
AACATTCTTC TGGATCAATC AGAATGCAGT CTGGTGCGCC GGGAGGCTGC ATTTATTCTT 
CAGAATCTCC TTGTAATTCC AATGCCTTCA GAAATTATAA AGGATTATAC TTGGCAGGGC 
CCCTGTGTCC ATGATGAGGA TTCTGGCCTG TCACTTTCAG GAAAACCTGC CCTTCAGGCT 
CTTTTATATC ACTGTCGTTT CTATGAACAT TTGACTCACA TGGTGAAACA TTGTTATGTA 
GGAAGATATA TCTTTGATTT TAATTTTTCT GCTTTTACTG AAACTTCAGA ATACAGTGAT 
TTAAATGGTT TAGACGACTC ATTCAAGTTT TGGAGGGCCC CTTCTGGGAC ATCTACTCAG 
GATCATGAGC CAAGCTCTCT TTCCACCTCA GAAACAATGG TGGCTGCCTC GGTGGTCAGT 
TCTGAGATTC AGCCCCTGCT GCCGTCAACA CTTCTTCCTG AAACTGCCCT TGACCAGTTT 
GTGACGCAAG GTCAACGTGA AATGAGACCA GTGCGACCTC GTGACTCATT GCTGTCTACT 
TCCCTGAACA GGCAGTATGT GTTTGTTACT CCGTCTCTGC TGTCAGCAGT CTGTAGCCTT 
TTAGACAACC TCTTGGCAGT TACTCCAAGA GATGCTGCAA ATGCTTTTCA ACAAGCTCAT 
CTCATAGAAC TCCTCTGTAG CACGGTGGAT GCTTCCCTCA TAGAAATGTG CATCCAAGAA 
CTCAAGACCC CACTGCCTTT ACCATCTGCT GTTGAACATA TTCAGGCTCA GGCTTCCTTT 
TTCTTGGAAT ACCTATCCTC TTTGTCCCAG CTCTTGAAGT CCTGTCTGTT GGTAGAGCCT 
AAACTTGTGA CACAAGATGA GCTCTTGAAA CCTCTTATCA CTAATGTCAT CCGAGTTCTC 
ATTGTGTGTC CCAAAGATGT ATTAGATGTA CAGTTAGTGT TGGCTTTTTA CCGCACATGG 
ACGCATTTAT TTGACCTTCT GGCCACACTA CTGAGGAAAG CTGGTGCTGT CTCCCTTGTT 
CCTCTTACCT CAGCTCTTGC CAAGCATTGG GAGGCAGTGA CTGATACACT GTGTAGATGT 
GTGGGCCTCT CATTCAAGTG CCCTGCCCTG ACTATTGCCA GCCTCCAGTT CCTGTCTGTT 
CTCTTGGCTG AGGAAGAAAA GAGGCGTGTC CAGGACAAGG ACAAAACAAA TGAGGGCCAG 
GCCCCAACAG TGGCCTTACT TCTTGATGGA ACTCAGGGAA GCCTTAGCTC TTCAGAGCGA 
CTTAATGAAA CAATTCTTCA GTGCTATGAA GGAATATCCC CCAAAGATGT CCTGAAAAGA 
GTTGCTGCAA ATGCCCTGTT GTCACTTCTG GCTGTTAGCA GAAGAGCGCA GAGGCATGCT 
TTGAGATCTG ATCTCATAGA AAACTGTATG GAGCAGATGA AGCACATCAA TGCACAACTG 
AACTTAGATT TGCTGAGACC TGGGAAAGCA GTACTGAAAA AAAAGGAGGA TGGCTTCATT 
AAAGAATTAA GCATCACCAT GCAGCTCCTA AGAAACTGCC TTTATCAAAA TGAAGAATGT 
AAAGTGGCAG CTCTTGAAGC ACGTCTTGTG CCTGTATTGC ACTCTCTCTG GCCTTGGCTT 
TTGATGGAAG ATTCATTGAT GCAAATTGCC TTGCAGTTGC TTTGTGTCTA TACTGCAAAT 
TTTCCAGCTG GCTGCAGTTC CCTTTGTTGG TCAAATTATG GACAGTGTAT TCAAACTGCA 
CACAGAGGCA CCCCCAGTAG CTCCCTGATA CTGTGTATCC TGAAACTGGC TTCTCAGATG 
CCTGCAGAGA ACACCACCAT TCAGCAGTTG GTTTTTATGT TTCTTTCAAA CTTGGCCTTA 
TCTCATGACT GCAAGGGAGT AATTCAAAAG AGCAACTTTC TACAGCACTT CTTGTCCCTG 
ACATTGCCTA AAGGAGGAAA CCAACATCTC AGCACTGTGG CCATTCTGTG GTTGAAGTTG 
CTCCTGAATA TGTCATTTGG AGAAGATGGA CAGCAAATGA TCCTGAGGCT AGATGGCTGC 
TTAGACTTAC TCATAGAGAT GAGCAAGTAC AAGCACAAGA GCAGCCCTCA CATGCCTCTG 
CTGATCTTTC ATAACATTTG CTTCAGTCCT GCTAATAAGC CTAAAATCCT GGCTAATGAA 
AAAGTTGTTA CTCTTCTGGC TGCCTGTTTA GAAAGTGAGA ATCAAACTGC CCAGAGGATT 
GGAGCAGCTT CACTTTGGGC TCTAACTTAC AATTACCAAA AGGCAAAAGC AACTTTGAAA 
AATCCATCAA TAAAAAGAAA AGTAGATGAA GCATATTCCA TAGCAAAGAG AACTCTCTCA 
AACTCAGAAG AAAACCCTCT GAATTCCTAC TATTTGAAAT GCCTTGAAAA TCTAGTACAA 
CTGCTTAACT ATTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rttn ( XM_011247080.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245382.1 Mus musculus rotatin (Rttn), transcript variant X4, mRNA.

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CloneID	OMu74252
Clone ID Related Accession (Same CDS sequence)	XM_011247080.2 , XM_011247080.3
Accession Version	XM_011247080.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rotatin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011247080.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTCACC ATGCCTGGCT GCAAGACTGG AAATCTGCAC AGGCTAGTCC ATTACTCCAG 
GGAGAGAGTC AGAAGGTGTT TCTCCATATG CTGTCTCACC CATTGCCACA GGTGAAGGTG 
GAGACTTACC AGTGCTGCCT GGAGATTGTA AAGGAATGTT TAGGTGTCCA AAATGTCACT 
AAACCTGTGT CTTCACTTTG TAGTGGAATT CATTTTCTGC TTCATCCAAA AGTTCTGTAT 
GAAATCTCTG CATTTGGTAT TCAAGAATCC AAAAATGAGG TGAATGCTGC TGCCAAGGCC 
ATTCTGCTGT ATCTGCTTCA GGGACGATTG ATGATGACAG CTCTGACCTG GAACAAGTTT 
ATCGAATCTC TCGCTCCTGT CATTCCAGTG CTACAGGGCT ACGCTGATAC AGAAGATCCC 
TTAGGCAACT GCATTCTCCT TCTAAGCAAA GTGAGCTCTG AGGCAGGAGA AGGCGCTCTC 
CCCTGCACTG CCCGGCTGAA GTCTGTGCTA CGGCTTTTGC TAGTAAAAAA ACCTTCGGTT 
CGTTCACTAG CATTAAAACT TTTAGCATTT CATCTTACCA GTGAAGAAGG GGCTGATAAA 
AAGCGCCCGT TCATAGATGC TGGCGTTCTC TCCAGAGTTA CTAATCTGTT TATTGTGAAA 
AAACCTATTG ACCTTAAGTT GGATGACAGA AGAGAACTAG TTATTAAGTT GGAGACTGTT 
AAAAAGGTGT GTGACATATT CACCTCAGAC GATGTTGATC TTGTTTTAAG AAAGTCAGCT 
GCAGAGCAAC TAGCTGTGAT AATGCAAGAT ATTAAAATGC ATACAGTGGT GAAAAAGTTA 
TGCTTAGTTG AGAAGATTAT TGGACATTTA AATGAATTTG TGGGTCAAGA TGGTGAGGTT 
ATAGAATGCT TGATTCAGCC ATGCCTCACA CTCCTGAGGA AGGTTTTGTA CGCTGACCCG 
GTTATCCGTG TTTCCTTGTC ACAGCAGGCC TCTCTCTTGA CCCTATTACT CAGAGTTTCC 
TTGATATTTC ATGAAGATTG TACTGTGGTG GCTGAAGTCG GGGCATTGTT TTGTCTTCTA 
TTGTTCGATG AAGTATCAAG AATGGATACA TGGTCTGATA CTCTTTCTGA TAAGCCTTCT 
TTGTCATCAG TCTTCAGTTT GCCGGTTTCA GTCTTTAGAA GGTATCATCT ACCAGTTCAT 
GTGACTGGCC ACCATGCTGT GAGTCCTTAC TCAATAGTTT TGCCATTACC TACTGAATGT 
TTGACCCTGA AGCCGGTGTC AGATATGCTG AGAATAGCTT GGAACCTGGC ATGGTATCAT 
GGGAGTGATA ATCTATTGAA ACAAATGACC TCTGAAGCTA AAATTCAGGA ATTTTTAGAT 
ACACTGCAGC TGTCCACAGA AGATATCCTT GCACTGAAGA TCACATACAC GGATAGTGGG 
TTGAAGGACT GCCTTCAGTC CATCATTCAG GCTGGAGGCC ACAGGGATGT GAGGGCTGCT 
ATCACCAGGA TGAGTTTTTA TCTTTTGAAT GACAAACTGT CTTTGAAGGA TGATATTGGT 
CCATGTGGCG TTACTTTAAA ATCCTTGGCT TGGCACACTG TGCTGAACAG GTTTTTACAA 
GTACTTCCTG CTTGCACTGA AGATGAGAAA CTGCTAATAG ATATCATACA TTTTTTGAAT 
AAGTTAATAA AGGAACAAAG AAAAAACCCA TCAATTGAAC TTTTAAACTG GATTCTGGAA 
TTACTTCTTA GGCATAATCC AAGTCCACTA CTAGACCTGC TGGTTCTGAC AGATTCACAT 
GCAGGAGAAG AAACAGATGA CGTCCGGACT GCTGTCAGAC AGCAACTCCA GAAAGAACTG 
ATCAGTCTTC TCAATTCCTT GTTACTGGTT TTTATGACAG TCTCTGACAG GAAATGCTTG 
GAACTGTTTT ACGTTTTCCA AACACAGCTG TCTCTGAAGC TACTCCAGTG CCTCAGAGTC 
ACAGATGCTC CCCATTTCTA TGGCTTACCT TCATTGGAGA GGACCTTAGC AGGGATGGCT 
CACCTCACTG CCTTCCCTGG GTGGAGTGCA CACTCTCCTC TTTCAAAGCC ACTTGAGATT 
TGTGGGAAGT ACTTGTCCGG TCTCCTGGAG GTCATTACTT CCTTTTATGT TGAACGGGGA 
GGAAATGCTA TGTCCTTCAT GGGGAAAGGC GTTACAAAGA GCACAGGTCT TTGCTTACTT 
CACTTATCCC ATGAGATGAT AGTCCAGGCA CCAGGCTCGG AGTGGATGCC GCTTTGGTTC 
TTGCCTTTGG GTAGTCACAG TGAAGAACAT GCCCCTACTC AGCAAGGATT GGCCTGGCTA 
ATTCCCTTAT GGGTTGATAG AGACCCAGAG GTGAGATTCA CTTCACTGGG GTTGGGATCA 
GCACTGACTA CTATTGAAGC TGGCTGTGTG GCCTTGGCAA ACAGTTGTCA GAATATTTCT 
GGTGGGCTCT GGGGAACCGT GGTGAACATT CTTCTGGATC AATCAGAATG CAGTCTGGTG 
CGCCGGGAGG CTGCATTTAT TCTTCAGAAT CTCCTTGTAA TTCCAATGCC TTCAGAAATT 
ATAAAGGATT ATACTTGGCA GGGCCCCTGT GTCCATGATG AGGATTCTGG CCTGTCACTT 
TCAGGAAAAC CTGCCCTTCA GGCTCTTTTA TATCACTGTC GTTTCTATGA ACATTTGACT 
CACATGGTGA AACATTGTTA TGTAGGAAGA TATATCTTTG ATTTTAATTT TTCTGCTTTT 
ACTGAAACTT CAGAATACAG TGATTTAAAT GGTTTAGACG ACTCATTCAA GTTTTGGAGG 
GCCCCTTCTG GGACATCTAC TCAGGATCAT GAGCCAAGCT CTCTTTCCAC CTCAGAAACA 
ATGGTGGCTG CCTCGGTGGT CAGTTCTGAG ATTCAGCCCC TGCTGCCGTC AACACTTCTT 
CCTGAAACTG CCCTTGACCA GTTTGTGACG CAAGGTCAAC GTGAAATGAG ACCAGTGCGA 
CCTCGTGACT CATTGCTGTC TACTTCCCTG AACAGGCAGT ATGTGTTTGT TACTCCGTCT 
CTGCTGTCAG CAGTCTGTAG CCTTTTAGAC AACCTCTTGG CAGTTACTCC AAGAGATGCT 
GCAAATGCTT TTCAACAAGC TCATCTCATA GAACTCCTCT GTAGCACGGT GGATGCTTCC 
CTCATAGAAA TGTGCATCCA AGAACTCAAG ACCCCACTGC CTTTACCATC TGCTGTTGAA 
CATATTCAGG CTCAGGCTTC CTTTTTCTTG GAATACCTAT CCTCTTTGTC CCAGCTCTTG 
AAGTCCTGTC TGTTGGTAGA GCCTAAACTT GTGACACAAG ATGAGCTCTT GAAACCTCTT 
ATCACTAATG TCATCCGAGT TCTCATTGTG TGTCCCAAAG ATGTATTAGA TGTACAGTTA 
GTGTTGGCTT TTTACCGCAC ATGGACGCAT TTATTTGACC TTCTGGCCAC ACTACTGAGG 
AAAGCTGGTG CTGTCTCCCT TGTTCCTCTT ACCTCAGCTC TTGCCAAGCA TTGGGAGGCA 
GTGACTGATA CACTGTGTAG ATGTGTGGGC CTCTCATTCA AGTGCCCTGC CCTGACTATT 
GCCAGCCTCC AGTTCCTGTC TGTTCTCTTG GCTGAGGAAG AAAAGAGGCG TGTCCAGGAC 
AAGGACAAAA CAAATGAGGG CCAGGCCCCA ACAGTGGCCT TACTTCTTGA TGGAACTCAG 
GGAAGCCTTA GCTCTTCAGA GCGACTTAAT GAAACAATTC TTCAGTGCTA TGAAGGAATA 
TCCCCCAAAG ATGTCCTGAA AAGAGTTGCT GCAAATGCCC TGTTGTCACT TCTGGCTGTT 
AGCAGAAGAG CGCAGAGGCA TGCTTTGAGA TCTGATCTCA TAGAAAACTG TATGGAGCAG 
ATGAAGCACA TCAATGCACA ACTGAACTTA GATTTGCTGA GACCTGGGAA AGCAGTACTG 
AAAAAAAAGG AGGATGGCTT CATTAAAGAA TTAAGCATCA CCATGCAGCT CCTAAGAAAC 
TGCCTTTATC AAAATGAAGA ATGTAAAGTG GCAGCTCTTG AAGCACGTCT TGTGCCTGTA 
TTGCACTCTC TCTGGCCTTG GCTTTTGATG GAAGATTCAT TGATGCAAAT TGCCTTGCAG 
TTGCTTTGTG TCTATACTGC AAATTTTCCA GCTGGCTGCA GTTCCCTTTG TTGGTCAAAT 
TATGGACAGT GTATTCAAAC TGCACACAGA GGCACCCCCA GTAGCTCCCT GATACTGTGT 
ATCCTGAAAC TGGCTTCTCA GATGCCTGCA GAGAACACCA CCATTCAGCA GTTGGTTTTT 
ATGTTTCTTT CAAACTTGGC CTTATCTCAT GACTGCAAGG GAGTAATTCA AAAGAGCAAC 
TTTCTACAGC ACTTCTTGTC CCTGACATTG CCTAAAGGAG GAAACCAACA TCTCAGCACT 
GTGGCCATTC TGTGGTTGAA GTTGCTCCTG AATATGTCAT TTGGAGAAGA TGGACAGCAA 
ATGATCCTGA GGCTAGATGG CTGCTTAGAC TTACTCATAG AGATGAGCAA GTACAAGCAC 
AAGAGCAGCC CTCACATGCC TCTGCTGATC TTTCATAACA TTTGCTTCAG TCCTGCTAAT 
AAGCCTAAAA TCCTGGCTAA TGAAAAAGTT GTTACTCTTC TGGCTGCCTG TTTAGAAAGT 
GAGAATCAAA CTGCCCAGAG GATTGGAGCA GCTTCACTTT GGGCTCTAAC TTACAATTAC 
CAAAAGGCAA AAGCAACTTT GAAAAATCCA TCAATAAAAA GAAAAGTAGA TGAAGCATAT 
TCCATAGCAA AGAGAACTCT CTCAAACTCA GAAGAAAACC CTCTGAATTC CTACTATTTG 
AAATGCCTTG AAAATCTAGT ACAACTGCTT AACTATTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245382.1
CDS	1280..6181
Translation	MCHHAWLQDWKSAQASPLLQGESQKVFLHMLSHPLPQVKVETYQCCLEIVKECLGVQNVTKPVSSLCSGIHFLLHPKVLYEISAFGIQESKNEVNAAAKAILLYLLQGRLMMTALTWNKFIESLAPVIPVLQGYADTEDPLGNCILLLSKVSSEAGEGALPCTARLKSVLRLLLVKKPSVRSLALKLLAFHLTSEEGADKKRPFIDAGVLSRVTNLFIVKKPIDLKLDDRRELVIKLETVKKVCDIFTSDDVDLVLRKSAAEQLAVIMQDIKMHTVVKKLCLVEKIIGHLNEFVGQDGEVIECLIQPCLTLLRKVLYADPVIRVSLSQQASLLTLLLRVSLIFHEDCTVVAEVGALFCLLLFDEVSRMDTWSDTLSDKPSLSSVFSLPVSVFRRYHLPVHVTGHHAVSPYSIVLPLPTECLTLKPVSDMLRIAWNLAWYHGSDNLLKQMTSEAKIQEFLDTLQLSTEDILALKITYTDSGLKDCLQSIIQAGGHRDVRAAITRMSFYLLNDKLSLKDDIGPCGVTLKSLAWHTVLNRFLQVLPACTEDEKLLIDIIHFLNKLIKEQRKNPSIELLNWILELLLRHNPSPLLDLLVLTDSHAGEETDDVRTAVRQQLQKELISLLNSLLLVFMTVSDRKCLELFYVFQTQLSLKLLQCLRVTDAPHFYGLPSLERTLAGMAHLTAFPGWSAHSPLSKPLEICGKYLSGLLEVITSFYVERGGNAMSFMGKGVTKSTGLCLLHLSHEMIVQAPGSEWMPLWFLPLGSHSEEHAPTQQGLAWLIPLWVDRDPEVRFTSLGLGSALTTIEAGCVALANSCQNISGGLWGTVVNILLDQSECSLVRREAAFILQNLLVIPMPSEIIKDYTWQGPCVHDEDSGLSLSGKPALQALLYHCRFYEHLTHMVKHCYVGRYIFDFNFSAFTETSEYSDLNGLDDSFKFWRAPSGTSTQDHEPSSLSTSETMVAASVVSSEIQPLLPSTLLPETALDQFVTQGQREMRPVRPRDSLLSTSLNRQYVFVTPSLLSAVCSLLDNLLAVTPRDAANAFQQAHLIELLCSTVDASLIEMCIQELKTPLPLPSAVEHIQAQASFFLEYLSSLSQLLKSCLLVEPKLVTQDELLKPLITNVIRVLIVCPKDVLDVQLVLAFYRTWTHLFDLLATLLRKAGAVSLVPLTSALAKHWEAVTDTLCRCVGLSFKCPALTIASLQFLSVLLAEEEKRRVQDKDKTNEGQAPTVALLLDGTQGSLSSSERLNETILQCYEGISPKDVLKRVAANALLSLLAVSRRAQRHALRSDLIENCMEQMKHINAQLNLDLLRPGKAVLKKKEDGFIKELSITMQLLRNCLYQNEECKVAALEARLVPVLHSLWPWLLMEDSLMQIALQLLCVYTANFPAGCSSLCWSNYGQCIQTAHRGTPSSSLILCILKLASQMPAENTTIQQLVFMFLSNLALSHDCKGVIQKSNFLQHFLSLTLPKGGNQHLSTVAILWLKLLLNMSFGEDGQQMILRLDGCLDLLIEMSKYKHKSSPHMPLLIFHNICFSPANKPKILANEKVVTLLAACLESENQTAQRIGAASLWALTYNYQKAKATLKNPSIKRKVDEAYSIAKRTLSNSEENPLNSYYLKCLENLVQLLNYS

Target ORF information:

RefSeq Version	XM_011247080.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus rotatin (Rttn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247080.3

ORF Insert Sequence:

ATGTGTCACC ATGCCTGGCT GCAAGACTGG AAATCTGCAC AGGCTAGTCC ATTACTCCAG 
GGAGAGAGTC AGAAGGTGTT TCTCCATATG CTGTCTCACC CATTGCCACA GGTGAAGGTG 
GAGACTTACC AGTGCTGCCT GGAGATTGTA AAGGAATGTT TAGGTGTCCA AAATGTCACT 
AAACCTGTGT CTTCACTTTG TAGTGGAATT CATTTTCTGC TTCATCCAAA AGTTCTGTAT 
GAAATCTCTG CATTTGGTAT TCAAGAATCC AAAAATGAGG TGAATGCTGC TGCCAAGGCC 
ATTCTGCTGT ATCTGCTTCA GGGACGATTG ATGATGACAG CTCTGACCTG GAACAAGTTT 
ATCGAATCTC TCGCTCCTGT CATTCCAGTG CTACAGGGCT ACGCTGATAC AGAAGATCCC 
TTAGGCAACT GCATTCTCCT TCTAAGCAAA GTGAGCTCTG AGGCAGGAGA AGGCGCTCTC 
CCCTGCACTG CCCGGCTGAA GTCTGTGCTA CGGCTTTTGC TAGTAAAAAA ACCTTCGGTT 
CGTTCACTAG CATTAAAACT TTTAGCATTT CATCTTACCA GTGAAGAAGG GGCTGATAAA 
AAGCGCCCGT TCATAGATGC TGGCGTTCTC TCCAGAGTTA CTAATCTGTT TATTGTGAAA 
AAACCTATTG ACCTTAAGTT GGATGACAGA AGAGAACTAG TTATTAAGTT GGAGACTGTT 
AAAAAGGTGT GTGACATATT CACCTCAGAC GATGTTGATC TTGTTTTAAG AAAGTCAGCT 
GCAGAGCAAC TAGCTGTGAT AATGCAAGAT ATTAAAATGC ATACAGTGGT GAAAAAGTTA 
TGCTTAGTTG AGAAGATTAT TGGACATTTA AATGAATTTG TGGGTCAAGA TGGTGAGGTT 
ATAGAATGCT TGATTCAGCC ATGCCTCACA CTCCTGAGGA AGGTTTTGTA CGCTGACCCG 
GTTATCCGTG TTTCCTTGTC ACAGCAGGCC TCTCTCTTGA CCCTATTACT CAGAGTTTCC 
TTGATATTTC ATGAAGATTG TACTGTGGTG GCTGAAGTCG GGGCATTGTT TTGTCTTCTA 
TTGTTCGATG AAGTATCAAG AATGGATACA TGGTCTGATA CTCTTTCTGA TAAGCCTTCT 
TTGTCATCAG TCTTCAGTTT GCCGGTTTCA GTCTTTAGAA GGTATCATCT ACCAGTTCAT 
GTGACTGGCC ACCATGCTGT GAGTCCTTAC TCAATAGTTT TGCCATTACC TACTGAATGT 
TTGACCCTGA AGCCGGTGTC AGATATGCTG AGAATAGCTT GGAACCTGGC ATGGTATCAT 
GGGAGTGATA ATCTATTGAA ACAAATGACC TCTGAAGCTA AAATTCAGGA ATTTTTAGAT 
ACACTGCAGC TGTCCACAGA AGATATCCTT GCACTGAAGA TCACATACAC GGATAGTGGG 
TTGAAGGACT GCCTTCAGTC CATCATTCAG GCTGGAGGCC ACAGGGATGT GAGGGCTGCT 
ATCACCAGGA TGAGTTTTTA TCTTTTGAAT GACAAACTGT CTTTGAAGGA TGATATTGGT 
CCATGTGGCG TTACTTTAAA ATCCTTGGCT TGGCACACTG TGCTGAACAG GTTTTTACAA 
GTACTTCCTG CTTGCACTGA AGATGAGAAA CTGCTAATAG ATATCATACA TTTTTTGAAT 
AAGTTAATAA AGGAACAAAG AAAAAACCCA TCAATTGAAC TTTTAAACTG GATTCTGGAA 
TTACTTCTTA GGCATAATCC AAGTCCACTA CTAGACCTGC TGGTTCTGAC AGATTCACAT 
GCAGGAGAAG AAACAGATGA CGTCCGGACT GCTGTCAGAC AGCAACTCCA GAAAGAACTG 
ATCAGTCTTC TCAATTCCTT GTTACTGGTT TTTATGACAG TCTCTGACAG GAAATGCTTG 
GAACTGTTTT ACGTTTTCCA AACACAGCTG TCTCTGAAGC TACTCCAGTG CCTCAGAGTC 
ACAGATGCTC CCCATTTCTA TGGCTTACCT TCATTGGAGA GGACCTTAGC AGGGATGGCT 
CACCTCACTG CCTTCCCTGG GTGGAGTGCA CACTCTCCTC TTTCAAAGCC ACTTGAGATT 
TGTGGGAAGT ACTTGTCCGG TCTCCTGGAG GTCATTACTT CCTTTTATGT TGAACGGGGA 
GGAAATGCTA TGTCCTTCAT GGGGAAAGGC GTTACAAAGA GCACAGGTCT TTGCTTACTT 
CACTTATCCC ATGAGATGAT AGTCCAGGCA CCAGGCTCGG AGTGGATGCC GCTTTGGTTC 
TTGCCTTTGG GTAGTCACAG TGAAGAACAT GCCCCTACTC AGCAAGGATT GGCCTGGCTA 
ATTCCCTTAT GGGTTGATAG AGACCCAGAG GTGAGATTCA CTTCACTGGG GTTGGGATCA 
GCACTGACTA CTATTGAAGC TGGCTGTGTG GCCTTGGCAA ACAGTTGTCA GAATATTTCT 
GGTGGGCTCT GGGGAACCGT GGTGAACATT CTTCTGGATC AATCAGAATG CAGTCTGGTG 
CGCCGGGAGG CTGCATTTAT TCTTCAGAAT CTCCTTGTAA TTCCAATGCC TTCAGAAATT 
ATAAAGGATT ATACTTGGCA GGGCCCCTGT GTCCATGATG AGGATTCTGG CCTGTCACTT 
TCAGGAAAAC CTGCCCTTCA GGCTCTTTTA TATCACTGTC GTTTCTATGA ACATTTGACT 
CACATGGTGA AACATTGTTA TGTAGGAAGA TATATCTTTG ATTTTAATTT TTCTGCTTTT 
ACTGAAACTT CAGAATACAG TGATTTAAAT GGTTTAGACG ACTCATTCAA GTTTTGGAGG 
GCCCCTTCTG GGACATCTAC TCAGGATCAT GAGCCAAGCT CTCTTTCCAC CTCAGAAACA 
ATGGTGGCTG CCTCGGTGGT CAGTTCTGAG ATTCAGCCCC TGCTGCCGTC AACACTTCTT 
CCTGAAACTG CCCTTGACCA GTTTGTGACG CAAGGTCAAC GTGAAATGAG ACCAGTGCGA 
CCTCGTGACT CATTGCTGTC TACTTCCCTG AACAGGCAGT ATGTGTTTGT TACTCCGTCT 
CTGCTGTCAG CAGTCTGTAG CCTTTTAGAC AACCTCTTGG CAGTTACTCC AAGAGATGCT 
GCAAATGCTT TTCAACAAGC TCATCTCATA GAACTCCTCT GTAGCACGGT GGATGCTTCC 
CTCATAGAAA TGTGCATCCA AGAACTCAAG ACCCCACTGC CTTTACCATC TGCTGTTGAA 
CATATTCAGG CTCAGGCTTC CTTTTTCTTG GAATACCTAT CCTCTTTGTC CCAGCTCTTG 
AAGTCCTGTC TGTTGGTAGA GCCTAAACTT GTGACACAAG ATGAGCTCTT GAAACCTCTT 
ATCACTAATG TCATCCGAGT TCTCATTGTG TGTCCCAAAG ATGTATTAGA TGTACAGTTA 
GTGTTGGCTT TTTACCGCAC ATGGACGCAT TTATTTGACC TTCTGGCCAC ACTACTGAGG 
AAAGCTGGTG CTGTCTCCCT TGTTCCTCTT ACCTCAGCTC TTGCCAAGCA TTGGGAGGCA 
GTGACTGATA CACTGTGTAG ATGTGTGGGC CTCTCATTCA AGTGCCCTGC CCTGACTATT 
GCCAGCCTCC AGTTCCTGTC TGTTCTCTTG GCTGAGGAAG AAAAGAGGCG TGTCCAGGAC 
AAGGACAAAA CAAATGAGGG CCAGGCCCCA ACAGTGGCCT TACTTCTTGA TGGAACTCAG 
GGAAGCCTTA GCTCTTCAGA GCGACTTAAT GAAACAATTC TTCAGTGCTA TGAAGGAATA 
TCCCCCAAAG ATGTCCTGAA AAGAGTTGCT GCAAATGCCC TGTTGTCACT TCTGGCTGTT 
AGCAGAAGAG CGCAGAGGCA TGCTTTGAGA TCTGATCTCA TAGAAAACTG TATGGAGCAG 
ATGAAGCACA TCAATGCACA ACTGAACTTA GATTTGCTGA GACCTGGGAA AGCAGTACTG 
AAAAAAAAGG AGGATGGCTT CATTAAAGAA TTAAGCATCA CCATGCAGCT CCTAAGAAAC 
TGCCTTTATC AAAATGAAGA ATGTAAAGTG GCAGCTCTTG AAGCACGTCT TGTGCCTGTA 
TTGCACTCTC TCTGGCCTTG GCTTTTGATG GAAGATTCAT TGATGCAAAT TGCCTTGCAG 
TTGCTTTGTG TCTATACTGC AAATTTTCCA GCTGGCTGCA GTTCCCTTTG TTGGTCAAAT 
TATGGACAGT GTATTCAAAC TGCACACAGA GGCACCCCCA GTAGCTCCCT GATACTGTGT 
ATCCTGAAAC TGGCTTCTCA GATGCCTGCA GAGAACACCA CCATTCAGCA GTTGGTTTTT 
ATGTTTCTTT CAAACTTGGC CTTATCTCAT GACTGCAAGG GAGTAATTCA AAAGAGCAAC 
TTTCTACAGC ACTTCTTGTC CCTGACATTG CCTAAAGGAG GAAACCAACA TCTCAGCACT 
GTGGCCATTC TGTGGTTGAA GTTGCTCCTG AATATGTCAT TTGGAGAAGA TGGACAGCAA 
ATGATCCTGA GGCTAGATGG CTGCTTAGAC TTACTCATAG AGATGAGCAA GTACAAGCAC 
AAGAGCAGCC CTCACATGCC TCTGCTGATC TTTCATAACA TTTGCTTCAG TCCTGCTAAT 
AAGCCTAAAA TCCTGGCTAA TGAAAAAGTT GTTACTCTTC TGGCTGCCTG TTTAGAAAGT 
GAGAATCAAA CTGCCCAGAG GATTGGAGCA GCTTCACTTT GGGCTCTAAC TTACAATTAC 
CAAAAGGCAA AAGCAACTTT GAAAAATCCA TCAATAAAAA GAAAAGTAGA TGAAGCATAT 
TCCATAGCAA AGAGAACTCT CTCAAACTCA GAAGAAAACC CTCTGAATTC CTACTATTTG 
AAATGCCTTG AAAATCTAGT ACAACTGCTT AACTATTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rttn ( XM_006526477.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526540.1 Mus musculus rotatin (Rttn), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu54948
Clone ID Related Accession (Same CDS sequence)	XM_006526477.3 , XM_030250463.1
Accession Version	XM_006526477.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rotatin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526477.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAATAA GAGTTCTGGA ACTGCTTGCA GAGGACATGC TGCTCATTGG ACAAGCAATT 
TCATCAGAAA TCTGGGATGA TAACAGCCTT TTTGCTATCA ATGTGAAAGA GAAACTGCTC 
CAAGTGTTGG GGTCCCTTGG AGAAACCATG TGCTACCGTA AAACCAGTTT TAGCATGGAG 
CAAGCCGAGG CTGTACTCGT GCACCACAGA ATGGCCTTTG TCAGCATCTC ACTGTTCACA 
GTTCGACTGT TGCAACTTCT TCTCCCTGTT GAAAAGGCGA GTGAGTTTTT GCCAGAGTCC 
ATGTCAGCAG CATTATTTTT TGTCTCCTTG GACATGCCTA TTTCTTTGGA ATATCCAGAG 
ATTCATGACA CAGTTGTGGC TTATTTGGAA CAGCTGGATT CTGAAAATTA CAGCATTTAT 
AAGCGAACTG CAGAGGCTGT TTATTCAATT GAATGCACCT GTAACTTCCT ATCTGATGTG 
GGGAAAGAGG GAGAAAAGAA TCTTCTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTCTCCCCTA ATAAAGGAAA TCATCTGCAT CTGTTCAAAG 
ATCTGGAAAT CTGCACAGGC TAGTCCATTA CTCCAGGGAG AGAGTCAGAA GGTGTTTCTC 
CATATGCTGT CTCACCCATT GCCACAGGTG AAGGTGGAGA CTTACCAGTG CTGCCTGGAG 
ATTGTAAAGG AATGTTTAGG TGTCCAAAAT GTCACTAAAC CTGTGTCTTC ACTTTGTAGT 
GGAATTCATT TTCTGCTTCA TCCAAAAGTT CTGTATGAAA TCTCTGCATT TGGTATTCAA 
GAATCCAAAA ATGAGGTGAA TGCTGCTGCC AAGGCCATTC TGCTGTATCT GCTTCAGGGA 
CGATTGATGA TGACAGCTCT GACCTGGAAC AAGTTTATCG AATCTCTCGC TCCTGTCATT 
CCAGTGCTAC AGGGCTACGC TGATACAGAA GATCCCTTAG GCAACTGCAT TCTCCTTCTA 
AGCAAAGTGA GCTCTGAGGC AGGAGAAGGC GCTCTCCCCT GCACTGCCCG GCTGAAGTCT 
GTGCTACGGC TTTTGCTAGT AAAAAAACCT TCGGTTCGTT CACTAGCATT AAAACTTTTA 
GCATTTCATC TTACCAGTGA AGAAGGGGCT GATAAAAAGC GCCCGTTCAT AGATGCTGGC 
GTTCTCTCCA GAGTTACTAA TCTGTTTATT GTGAAAAAAC CTATTGACCT TAAGTTGGAT 
GACAGAAGAG AACTAGTTAT TAAGTTGGAG ACTGTTAAAA AGGTGTGTGA CATATTCACC 
TCAGACGATG TTGATCTTGT TTTAAGAAAG TCAGCTGCAG AGCAACTAGC TGTGATAATG 
CAAGATATTA AAATGCATAC AGTGGTGAAA AAGTTATGCT TAGTTGAGAA GATTATTGGA 
CATTTAAATG AATTTGTGGG TCAAGATGGT GAGGTTATAG AATGCTTGAT TCAGCCATGC 
CTCACACTCC TGAGGAAGGT TTTGTACGCT GACCCGGTTA TCCGTGTTTC CTTGTCACAG 
CAGGCCTCTC TCTTGACCCT ATTACTCAGA GTTTCCTTGA TATTTCATGA AGATTGTACT 
GTGGTGGCTG AAGTCGGGGC ATTGTTTTGT CTTCTATTGT TCGATGAAGT ATCAAGAATG 
GATACATGGT CTGATACTCT TTCTGATAAG CCTTCTTTGT CATCAGTCTT CAGTTTGCCG 
GTTTCAGTCT TTAGAAGGTA TCATCTACCA GTTCATGTGA CTGGCCACCA TGCTGTGAGT 
CCTTACTCAA TAGTTTTGCC ATTACCTACT GAATGTTTGA CCCTGAAGCC GGTGTCAGAT 
ATGCTGAGAA TAGCTTGGAA CCTGGCATGG TATCATGGGA GTGATAATCT ATTGAAACAA 
ATGACCTCTG AAGCTAAAAT TCAGGAATTT TTAGATACAC TGCAGCTGTC CACAGAAGAT 
ATCCTTGCAC TGAAGATCAC ATACACGGAT AGTGGGTTGA AGGACTGCCT TCAGTCCATC 
ATTCAGGCTG GAGGCCACAG GGATGTGAGG GCTGCTATCA CCAGGATGAG TTTTTATCTT 
TTGAATGACA AACTGTCTTT GAAGGATGAT ATTGGTCCAT GTGGCGTTAC TTTAAAATCC 
TTGGCTTGGC ACACTGTGCT GAACAGGTTT TTACAAGTAC TTCCTGCTTG CACTGAAGAT 
GAGAAACTGC TAATAGATAT CATACATTTT TTGAATAAGT TAATAAAGGA ACAAAGAAAA 
AACCCATCAA TTGAACTTTT AAACTGGATT CTGGAATTAC TTCTTAGGCA TAATCCAAGT 
CCACTACTAG ACCTGCTGGT TCTGACAGAT TCACATGCAG GAGAAGAAAC AGATGACGTC 
CGGACTGCTG TCAGACAGCA ACTCCAGAAA GAACTGATCA GTCTTCTCAA TTCCTTGTTA 
CTGGTTTTTA TGACAGTCTC TGACAGGAAA TGCTTGGAAC TGTTTTACGT TTTCCAAACA 
CAGCTGTCTC TGAAGCTACT CCAGTGCCTC AGAGTCACAG ATGCTCCCCA TTTCTATGGC 
TTACCTTCAT TGGAGAGGAC CTTAGCAGGG ATGGCTCACC TCACTGCCTT CCCTGGGTGG 
AGTGCACACT CTCCTCTTTC AAAGCCACTT GAGATTTGTG GGAAGTACTT GTCCGGTCTC 
CTGGAGGTCA TTACTTCCTT TTATGTTGAA CGGGGAGGAA ATGCTATGTC CTTCATGGGG 
AAAGGCGTTA CAAAGAGCAC AGGTCTTTGC TTACTTCACT TATCCCATGA GATGATAGTC 
CAGGCACCAG GCTCGGAGTG GATGCCGCTT TGGTTCTTGC CTTTGGGTAG TCACAGTGAA 
GAACATGCCC CTACTCAGCA AGGATTGGCC TGGCTAATTC CCTTATGGGT TGATAGAGAC 
CCAGAGGTGA GATTCACTTC ACTGGGGTTG GGATCAGCAC TGACTACTAT TGAAGCTGGC 
TGTGTGGCCT TGGCAAACAG TTGTCAGAAT ATTTCTGGTG GGCTCTGGGG AACCGTGGTG 
AACATTCTTC TGGATCAATC AGAATGCAGT CTGGTGCGCC GGGAGGCTGC ATTTATTCTT 
CAGAATCTCC TTGTAATTCC AATGCCTTCA GAAATTATAA AGGATTATAC TTGGCAGGGC 
CCCTGTGTCC ATGATGAGGA TTCTGGCCTG TCACTTTCAG GAAAACCTGC CCTTCAGGCT 
CTTTTATATC ACTGTCGTTT CTATGAACAT TTGACTCACA TGGTGAAACA TTGTTATGTA 
GGAAGATATA TCTTTGATTT TAATTTTTCT GCTTTTACTG AAACTTCAGA ATACAGTGAT 
TTAAATGGTT TAGACGACTC ATTCAAGTTT TGGAGGGCCC CTTCTGGGAC ATCTACTCAG 
GATCATGAGC CAAGCTCTCT TTCCACCTCA GAAACAATGG TGGCTGCCTC GGTGGTCAGT 
TCTGAGATTC AGCCCCTGCT GCCGTCAACA CTTCTTCCTG AAACTGCCCT TGACCAGTTT 
GTGACGCAAG GTCAACGTGA AATGAGACCA GTGCGACCTC GTGACTCATT GCTGTCTACT 
TCCCTGAACA GGCAGTATGT GTTTGTTACT CCGTCTCTGC TGTCAGCAGT CTGTAGCCTT 
TTAGACAACC TCTTGGCAGT TACTCCAAGA GATGCTGCAA ATGCTTTTCA ACAAGCTCAT 
CTCATAGAAC TCCTCTGTAG CACGGTGGAT GCTTCCCTCA TAGAAATGTG CATCCAAGAA 
CTCAAGACCC CACTGCCTTT ACCATCTGCT GTTGAACATA TTCAGGCTCA GGCTTCCTTT 
TTCTTGGAAT ACCTATCCTC TTTGTCCCAG CTCTTGAAGT CCTGTCTGTT GGTAGAGCCT 
AAACTTGTGA CACAAGATGA GCTCTTGAAA CCTCTTATCA CTAATGTCAT CCGAGTTCTC 
ATTGTGTGTC CCAAAGATGT ATTAGATGTA CAGTTAGTGT TGGCTTTTTA CCGCACATGG 
ACGCATTTAT TTGACCTTCT GGCCACACTA CTGAGGAAAG CTGGTGCTGT CTCCCTTGTT 
CCTCTTACCT CAGCTCTTGC CAAGCATTGG GAGGCAGTGA CTGATACACT GTGTAGATGT 
GTGGGCCTCT CATTCAAGTG CCCTGCCCTG ACTATTGCCA GCCTCCAGTT CCTGTCTGTT 
CTCTTGGCTG AGGAAGAAAA GAGGCGTGTC CAGGACAAGG ACAAAACAAA TGAGGGCCAG 
GCCCCAACAG TGGCCTTACT TCTTGATGGA ACTCAGGGAA GCCTTAGCTC TTCAGAGCGA 
CTTAATGAAA CAATTCTTCA GTGCTATGAA GGAATATCCC CCAAAGATGT CCTGAAAAGA 
GTTGCTGCAA ATGCCCTGTT GTCACTTCTG GCTGTTAGCA GAAGAGCGCA GAGGCATGCT 
TTGAGATCTG ATCTCATAGA AAACTGTATG GAGCAGATGA AGCACATCAA TGCACAACTG 
AACTTAGATT TGCTGAGACC TGGGAAAGCA GTACTGAAAA AAAAGGAGGA TGGCTTCATT 
AAAGAATTAA GCATCACCAT GCAGCTCCTA AGAAACTGCC TTTATCAAAA TGAAGAATGT 
AAAGTGGCAG CTCTTGAAGC ACGTCTTGTG CCTGTATTGC ACTCTCTCTG GCCTTGGCTT 
TTGATGGAAG ATTCATTGAT GCAAATTGCC TTGCAGTTGC TTTGTGTCTA TACTGCAAAT 
TTTCCAGCTG GCTGCAGTTC CCTTTGTTGG TCAAATTATG GACAGTGTAT TCAAACTGCA 
CACAGAGGCA CCCCCAGTAG CTCCCTGATA CTGTGTATCC TGAAACTGGC TTCTCAGATG 
CCTGCAGAGA ACACCACCAT TCAGCAGTTG GTTTTTATGT TTCTTTCAAA CTTGGCCTTA 
TCTCATGACT GCAAGGGAGT AATTCAAAAG AGCAACTTTC TACAGCACTT CTTGTCCCTG 
ACATTGCCTA AAGGAGGAAA CCAACATCTC AGCACTGTGG CCATTCTGTG GTTGAAGTTG 
CTCCTGAATA TGTCATTTGG AGAAGATGGA CAGCAAATGA TCCTGAGGCT AGATGGCTGC 
TTAGACTTAC TCATAGAGAT GAGCAAGTAC AAGCACAAGA GCAGCCCTCA CATGCCTCTG 
CTGATCTTTC ATAACATTTG CTTCAGTCCT GCTAATAAGC CTAAAATCCT GGCTAATGAA 
AAAGTTGTTA CTCTTCTGGC TGCCTGTTTA GAAAGTGAGA ATCAAACTGC CCAGAGGATT 
GGAGCAGCTT CACTTTGGGC TCTAACTTAC AATTACCAAA AGGCAAAAGC AACTTTGAAA 
AATCCATCAA TAAAAAGAAA AGTAGATGAA GCATATTCCA TAGCAAAGAG AACTCTCTCA 
AACTCAGAAG AAAACCCTCT GAATTCCTAC TATTTGAAAT GCCTTGAAAA TCTAGTACAA 
CTGCTTAACT ATTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526540.1
CDS	1050..6527
Translation	MIIRVLELLAEDMLLIGQAISSEIWDDNSLFAINVKEKLLQVLGSLGETMCYRKTSFSMEQAEAVLVHHRMAFVSISLFTVRLLQLLLPVEKASEFLPESMSAALFFVSLDMPISLEYPEIHDTVVAYLEQLDSENYSIYKRTAEAVYSIECTCNFLSDVGKEGEKNLLELVELADQALRSFSYHQHSPLIKEIICICSKIWKSAQASPLLQGESQKVFLHMLSHPLPQVKVETYQCCLEIVKECLGVQNVTKPVSSLCSGIHFLLHPKVLYEISAFGIQESKNEVNAAAKAILLYLLQGRLMMTALTWNKFIESLAPVIPVLQGYADTEDPLGNCILLLSKVSSEAGEGALPCTARLKSVLRLLLVKKPSVRSLALKLLAFHLTSEEGADKKRPFIDAGVLSRVTNLFIVKKPIDLKLDDRRELVIKLETVKKVCDIFTSDDVDLVLRKSAAEQLAVIMQDIKMHTVVKKLCLVEKIIGHLNEFVGQDGEVIECLIQPCLTLLRKVLYADPVIRVSLSQQASLLTLLLRVSLIFHEDCTVVAEVGALFCLLLFDEVSRMDTWSDTLSDKPSLSSVFSLPVSVFRRYHLPVHVTGHHAVSPYSIVLPLPTECLTLKPVSDMLRIAWNLAWYHGSDNLLKQMTSEAKIQEFLDTLQLSTEDILALKITYTDSGLKDCLQSIIQAGGHRDVRAAITRMSFYLLNDKLSLKDDIGPCGVTLKSLAWHTVLNRFLQVLPACTEDEKLLIDIIHFLNKLIKEQRKNPSIELLNWILELLLRHNPSPLLDLLVLTDSHAGEETDDVRTAVRQQLQKELISLLNSLLLVFMTVSDRKCLELFYVFQTQLSLKLLQCLRVTDAPHFYGLPSLERTLAGMAHLTAFPGWSAHSPLSKPLEICGKYLSGLLEVITSFYVERGGNAMSFMGKGVTKSTGLCLLHLSHEMIVQAPGSEWMPLWFLPLGSHSEEHAPTQQGLAWLIPLWVDRDPEVRFTSLGLGSALTTIEAGCVALANSCQNISGGLWGTVVNILLDQSECSLVRREAAFILQNLLVIPMPSEIIKDYTWQGPCVHDEDSGLSLSGKPALQALLYHCRFYEHLTHMVKHCYVGRYIFDFNFSAFTETSEYSDLNGLDDSFKFWRAPSGTSTQDHEPSSLSTSETMVAASVVSSEIQPLLPSTLLPETALDQFVTQGQREMRPVRPRDSLLSTSLNRQYVFVTPSLLSAVCSLLDNLLAVTPRDAANAFQQAHLIELLCSTVDASLIEMCIQELKTPLPLPSAVEHIQAQASFFLEYLSSLSQLLKSCLLVEPKLVTQDELLKPLITNVIRVLIVCPKDVLDVQLVLAFYRTWTHLFDLLATLLRKAGAVSLVPLTSALAKHWEAVTDTLCRCVGLSFKCPALTIASLQFLSVLLAEEEKRRVQDKDKTNEGQAPTVALLLDGTQGSLSSSERLNETILQCYEGISPKDVLKRVAANALLSLLAVSRRAQRHALRSDLIENCMEQMKHINAQLNLDLLRPGKAVLKKKEDGFIKELSITMQLLRNCLYQNEECKVAALEARLVPVLHSLWPWLLMEDSLMQIALQLLCVYTANFPAGCSSLCWSNYGQCIQTAHRGTPSSSLILCILKLASQMPAENTTIQQLVFMFLSNLALSHDCKGVIQKSNFLQHFLSLTLPKGGNQHLSTVAILWLKLLLNMSFGEDGQQMILRLDGCLDLLIEMSKYKHKSSPHMPLLIFHNICFSPANKPKILANEKVVTLLAACLESENQTAQRIGAASLWALTYNYQKAKATLKNPSIKRKVDEAYSIAKRTLSNSEENPLNSYYLKCLENLVQLLNYS

Target ORF information:

RefSeq Version	XM_006526477.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus rotatin (Rttn), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526477.3

ORF Insert Sequence:

ATGATAATAA GAGTTCTGGA ACTGCTTGCA GAGGACATGC TGCTCATTGG ACAAGCAATT 
TCATCAGAAA TCTGGGATGA TAACAGCCTT TTTGCTATCA ATGTGAAAGA GAAACTGCTC 
CAAGTGTTGG GGTCCCTTGG AGAAACCATG TGCTACCGTA AAACCAGTTT TAGCATGGAG 
CAAGCCGAGG CTGTACTCGT GCACCACAGA ATGGCCTTTG TCAGCATCTC ACTGTTCACA 
GTTCGACTGT TGCAACTTCT TCTCCCTGTT GAAAAGGCGA GTGAGTTTTT GCCAGAGTCC 
ATGTCAGCAG CATTATTTTT TGTCTCCTTG GACATGCCTA TTTCTTTGGA ATATCCAGAG 
ATTCATGACA CAGTTGTGGC TTATTTGGAA CAGCTGGATT CTGAAAATTA CAGCATTTAT 
AAGCGAACTG CAGAGGCTGT TTATTCAATT GAATGCACCT GTAACTTCCT ATCTGATGTG 
GGGAAAGAGG GAGAAAAGAA TCTTCTAGAA TTAGTGGAGC TGGCAGACCA AGCCTTGCGT 
AGCTTTTCCT ATCATCAGCA TTCTCCCCTA ATAAAGGAAA TCATCTGCAT CTGTTCAAAG 
ATCTGGAAAT CTGCACAGGC TAGTCCATTA CTCCAGGGAG AGAGTCAGAA GGTGTTTCTC 
CATATGCTGT CTCACCCATT GCCACAGGTG AAGGTGGAGA CTTACCAGTG CTGCCTGGAG 
ATTGTAAAGG AATGTTTAGG TGTCCAAAAT GTCACTAAAC CTGTGTCTTC ACTTTGTAGT 
GGAATTCATT TTCTGCTTCA TCCAAAAGTT CTGTATGAAA TCTCTGCATT TGGTATTCAA 
GAATCCAAAA ATGAGGTGAA TGCTGCTGCC AAGGCCATTC TGCTGTATCT GCTTCAGGGA 
CGATTGATGA TGACAGCTCT GACCTGGAAC AAGTTTATCG AATCTCTCGC TCCTGTCATT 
CCAGTGCTAC AGGGCTACGC TGATACAGAA GATCCCTTAG GCAACTGCAT TCTCCTTCTA 
AGCAAAGTGA GCTCTGAGGC AGGAGAAGGC GCTCTCCCCT GCACTGCCCG GCTGAAGTCT 
GTGCTACGGC TTTTGCTAGT AAAAAAACCT TCGGTTCGTT CACTAGCATT AAAACTTTTA 
GCATTTCATC TTACCAGTGA AGAAGGGGCT GATAAAAAGC GCCCGTTCAT AGATGCTGGC 
GTTCTCTCCA GAGTTACTAA TCTGTTTATT GTGAAAAAAC CTATTGACCT TAAGTTGGAT 
GACAGAAGAG AACTAGTTAT TAAGTTGGAG ACTGTTAAAA AGGTGTGTGA CATATTCACC 
TCAGACGATG TTGATCTTGT TTTAAGAAAG TCAGCTGCAG AGCAACTAGC TGTGATAATG 
CAAGATATTA AAATGCATAC AGTGGTGAAA AAGTTATGCT TAGTTGAGAA GATTATTGGA 
CATTTAAATG AATTTGTGGG TCAAGATGGT GAGGTTATAG AATGCTTGAT TCAGCCATGC 
CTCACACTCC TGAGGAAGGT TTTGTACGCT GACCCGGTTA TCCGTGTTTC CTTGTCACAG 
CAGGCCTCTC TCTTGACCCT ATTACTCAGA GTTTCCTTGA TATTTCATGA AGATTGTACT 
GTGGTGGCTG AAGTCGGGGC ATTGTTTTGT CTTCTATTGT TCGATGAAGT ATCAAGAATG 
GATACATGGT CTGATACTCT TTCTGATAAG CCTTCTTTGT CATCAGTCTT CAGTTTGCCG 
GTTTCAGTCT TTAGAAGGTA TCATCTACCA GTTCATGTGA CTGGCCACCA TGCTGTGAGT 
CCTTACTCAA TAGTTTTGCC ATTACCTACT GAATGTTTGA CCCTGAAGCC GGTGTCAGAT 
ATGCTGAGAA TAGCTTGGAA CCTGGCATGG TATCATGGGA GTGATAATCT ATTGAAACAA 
ATGACCTCTG AAGCTAAAAT TCAGGAATTT TTAGATACAC TGCAGCTGTC CACAGAAGAT 
ATCCTTGCAC TGAAGATCAC ATACACGGAT AGTGGGTTGA AGGACTGCCT TCAGTCCATC 
ATTCAGGCTG GAGGCCACAG GGATGTGAGG GCTGCTATCA CCAGGATGAG TTTTTATCTT 
TTGAATGACA AACTGTCTTT GAAGGATGAT ATTGGTCCAT GTGGCGTTAC TTTAAAATCC 
TTGGCTTGGC ACACTGTGCT GAACAGGTTT TTACAAGTAC TTCCTGCTTG CACTGAAGAT 
GAGAAACTGC TAATAGATAT CATACATTTT TTGAATAAGT TAATAAAGGA ACAAAGAAAA 
AACCCATCAA TTGAACTTTT AAACTGGATT CTGGAATTAC TTCTTAGGCA TAATCCAAGT 
CCACTACTAG ACCTGCTGGT TCTGACAGAT TCACATGCAG GAGAAGAAAC AGATGACGTC 
CGGACTGCTG TCAGACAGCA ACTCCAGAAA GAACTGATCA GTCTTCTCAA TTCCTTGTTA 
CTGGTTTTTA TGACAGTCTC TGACAGGAAA TGCTTGGAAC TGTTTTACGT TTTCCAAACA 
CAGCTGTCTC TGAAGCTACT CCAGTGCCTC AGAGTCACAG ATGCTCCCCA TTTCTATGGC 
TTACCTTCAT TGGAGAGGAC CTTAGCAGGG ATGGCTCACC TCACTGCCTT CCCTGGGTGG 
AGTGCACACT CTCCTCTTTC AAAGCCACTT GAGATTTGTG GGAAGTACTT GTCCGGTCTC 
CTGGAGGTCA TTACTTCCTT TTATGTTGAA CGGGGAGGAA ATGCTATGTC CTTCATGGGG 
AAAGGCGTTA CAAAGAGCAC AGGTCTTTGC TTACTTCACT TATCCCATGA GATGATAGTC 
CAGGCACCAG GCTCGGAGTG GATGCCGCTT TGGTTCTTGC CTTTGGGTAG TCACAGTGAA 
GAACATGCCC CTACTCAGCA AGGATTGGCC TGGCTAATTC CCTTATGGGT TGATAGAGAC 
CCAGAGGTGA GATTCACTTC ACTGGGGTTG GGATCAGCAC TGACTACTAT TGAAGCTGGC 
TGTGTGGCCT TGGCAAACAG TTGTCAGAAT ATTTCTGGTG GGCTCTGGGG AACCGTGGTG 
AACATTCTTC TGGATCAATC AGAATGCAGT CTGGTGCGCC GGGAGGCTGC ATTTATTCTT 
CAGAATCTCC TTGTAATTCC AATGCCTTCA GAAATTATAA AGGATTATAC TTGGCAGGGC 
CCCTGTGTCC ATGATGAGGA TTCTGGCCTG TCACTTTCAG GAAAACCTGC CCTTCAGGCT 
CTTTTATATC ACTGTCGTTT CTATGAACAT TTGACTCACA TGGTGAAACA TTGTTATGTA 
GGAAGATATA TCTTTGATTT TAATTTTTCT GCTTTTACTG AAACTTCAGA ATACAGTGAT 
TTAAATGGTT TAGACGACTC ATTCAAGTTT TGGAGGGCCC CTTCTGGGAC ATCTACTCAG 
GATCATGAGC CAAGCTCTCT TTCCACCTCA GAAACAATGG TGGCTGCCTC GGTGGTCAGT 
TCTGAGATTC AGCCCCTGCT GCCGTCAACA CTTCTTCCTG AAACTGCCCT TGACCAGTTT 
GTGACGCAAG GTCAACGTGA AATGAGACCA GTGCGACCTC GTGACTCATT GCTGTCTACT 
TCCCTGAACA GGCAGTATGT GTTTGTTACT CCGTCTCTGC TGTCAGCAGT CTGTAGCCTT 
TTAGACAACC TCTTGGCAGT TACTCCAAGA GATGCTGCAA ATGCTTTTCA ACAAGCTCAT 
CTCATAGAAC TCCTCTGTAG CACGGTGGAT GCTTCCCTCA TAGAAATGTG CATCCAAGAA 
CTCAAGACCC CACTGCCTTT ACCATCTGCT GTTGAACATA TTCAGGCTCA GGCTTCCTTT 
TTCTTGGAAT ACCTATCCTC TTTGTCCCAG CTCTTGAAGT CCTGTCTGTT GGTAGAGCCT 
AAACTTGTGA CACAAGATGA GCTCTTGAAA CCTCTTATCA CTAATGTCAT CCGAGTTCTC 
ATTGTGTGTC CCAAAGATGT ATTAGATGTA CAGTTAGTGT TGGCTTTTTA CCGCACATGG 
ACGCATTTAT TTGACCTTCT GGCCACACTA CTGAGGAAAG CTGGTGCTGT CTCCCTTGTT 
CCTCTTACCT CAGCTCTTGC CAAGCATTGG GAGGCAGTGA CTGATACACT GTGTAGATGT 
GTGGGCCTCT CATTCAAGTG CCCTGCCCTG ACTATTGCCA GCCTCCAGTT CCTGTCTGTT 
CTCTTGGCTG AGGAAGAAAA GAGGCGTGTC CAGGACAAGG ACAAAACAAA TGAGGGCCAG 
GCCCCAACAG TGGCCTTACT TCTTGATGGA ACTCAGGGAA GCCTTAGCTC TTCAGAGCGA 
CTTAATGAAA CAATTCTTCA GTGCTATGAA GGAATATCCC CCAAAGATGT CCTGAAAAGA 
GTTGCTGCAA ATGCCCTGTT GTCACTTCTG GCTGTTAGCA GAAGAGCGCA GAGGCATGCT 
TTGAGATCTG ATCTCATAGA AAACTGTATG GAGCAGATGA AGCACATCAA TGCACAACTG 
AACTTAGATT TGCTGAGACC TGGGAAAGCA GTACTGAAAA AAAAGGAGGA TGGCTTCATT 
AAAGAATTAA GCATCACCAT GCAGCTCCTA AGAAACTGCC TTTATCAAAA TGAAGAATGT 
AAAGTGGCAG CTCTTGAAGC ACGTCTTGTG CCTGTATTGC ACTCTCTCTG GCCTTGGCTT 
TTGATGGAAG ATTCATTGAT GCAAATTGCC TTGCAGTTGC TTTGTGTCTA TACTGCAAAT 
TTTCCAGCTG GCTGCAGTTC CCTTTGTTGG TCAAATTATG GACAGTGTAT TCAAACTGCA 
CACAGAGGCA CCCCCAGTAG CTCCCTGATA CTGTGTATCC TGAAACTGGC TTCTCAGATG 
CCTGCAGAGA ACACCACCAT TCAGCAGTTG GTTTTTATGT TTCTTTCAAA CTTGGCCTTA 
TCTCATGACT GCAAGGGAGT AATTCAAAAG AGCAACTTTC TACAGCACTT CTTGTCCCTG 
ACATTGCCTA AAGGAGGAAA CCAACATCTC AGCACTGTGG CCATTCTGTG GTTGAAGTTG 
CTCCTGAATA TGTCATTTGG AGAAGATGGA CAGCAAATGA TCCTGAGGCT AGATGGCTGC 
TTAGACTTAC TCATAGAGAT GAGCAAGTAC AAGCACAAGA GCAGCCCTCA CATGCCTCTG 
CTGATCTTTC ATAACATTTG CTTCAGTCCT GCTAATAAGC CTAAAATCCT GGCTAATGAA 
AAAGTTGTTA CTCTTCTGGC TGCCTGTTTA GAAAGTGAGA ATCAAACTGC CCAGAGGATT 
GGAGCAGCTT CACTTTGGGC TCTAACTTAC AATTACCAAA AGGCAAAAGC AACTTTGAAA 
AATCCATCAA TAAAAAGAAA AGTAGATGAA GCATATTCCA TAGCAAAGAG AACTCTCTCA 
AACTCAGAAG AAAACCCTCT GAATTCCTAC TATTTGAAAT GCCTTGAAAA TCTAGTACAA 
CTGCTTAACT ATTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rttn ( XM_011247080.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245382.1 Mus musculus rotatin (Rttn), transcript variant X4, mRNA.

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CloneID	OMu74252
Clone ID Related Accession (Same CDS sequence)	XM_011247080.2 , XM_011247080.3
Accession Version	XM_011247080.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rotatin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247080.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTCACC ATGCCTGGCT GCAAGACTGG AAATCTGCAC AGGCTAGTCC ATTACTCCAG 
GGAGAGAGTC AGAAGGTGTT TCTCCATATG CTGTCTCACC CATTGCCACA GGTGAAGGTG 
GAGACTTACC AGTGCTGCCT GGAGATTGTA AAGGAATGTT TAGGTGTCCA AAATGTCACT 
AAACCTGTGT CTTCACTTTG TAGTGGAATT CATTTTCTGC TTCATCCAAA AGTTCTGTAT 
GAAATCTCTG CATTTGGTAT TCAAGAATCC AAAAATGAGG TGAATGCTGC TGCCAAGGCC 
ATTCTGCTGT ATCTGCTTCA GGGACGATTG ATGATGACAG CTCTGACCTG GAACAAGTTT 
ATCGAATCTC TCGCTCCTGT CATTCCAGTG CTACAGGGCT ACGCTGATAC AGAAGATCCC 
TTAGGCAACT GCATTCTCCT TCTAAGCAAA GTGAGCTCTG AGGCAGGAGA AGGCGCTCTC 
CCCTGCACTG CCCGGCTGAA GTCTGTGCTA CGGCTTTTGC TAGTAAAAAA ACCTTCGGTT 
CGTTCACTAG CATTAAAACT TTTAGCATTT CATCTTACCA GTGAAGAAGG GGCTGATAAA 
AAGCGCCCGT TCATAGATGC TGGCGTTCTC TCCAGAGTTA CTAATCTGTT TATTGTGAAA 
AAACCTATTG ACCTTAAGTT GGATGACAGA AGAGAACTAG TTATTAAGTT GGAGACTGTT 
AAAAAGGTGT GTGACATATT CACCTCAGAC GATGTTGATC TTGTTTTAAG AAAGTCAGCT 
GCAGAGCAAC TAGCTGTGAT AATGCAAGAT ATTAAAATGC ATACAGTGGT GAAAAAGTTA 
TGCTTAGTTG AGAAGATTAT TGGACATTTA AATGAATTTG TGGGTCAAGA TGGTGAGGTT 
ATAGAATGCT TGATTCAGCC ATGCCTCACA CTCCTGAGGA AGGTTTTGTA CGCTGACCCG 
GTTATCCGTG TTTCCTTGTC ACAGCAGGCC TCTCTCTTGA CCCTATTACT CAGAGTTTCC 
TTGATATTTC ATGAAGATTG TACTGTGGTG GCTGAAGTCG GGGCATTGTT TTGTCTTCTA 
TTGTTCGATG AAGTATCAAG AATGGATACA TGGTCTGATA CTCTTTCTGA TAAGCCTTCT 
TTGTCATCAG TCTTCAGTTT GCCGGTTTCA GTCTTTAGAA GGTATCATCT ACCAGTTCAT 
GTGACTGGCC ACCATGCTGT GAGTCCTTAC TCAATAGTTT TGCCATTACC TACTGAATGT 
TTGACCCTGA AGCCGGTGTC AGATATGCTG AGAATAGCTT GGAACCTGGC ATGGTATCAT 
GGGAGTGATA ATCTATTGAA ACAAATGACC TCTGAAGCTA AAATTCAGGA ATTTTTAGAT 
ACACTGCAGC TGTCCACAGA AGATATCCTT GCACTGAAGA TCACATACAC GGATAGTGGG 
TTGAAGGACT GCCTTCAGTC CATCATTCAG GCTGGAGGCC ACAGGGATGT GAGGGCTGCT 
ATCACCAGGA TGAGTTTTTA TCTTTTGAAT GACAAACTGT CTTTGAAGGA TGATATTGGT 
CCATGTGGCG TTACTTTAAA ATCCTTGGCT TGGCACACTG TGCTGAACAG GTTTTTACAA 
GTACTTCCTG CTTGCACTGA AGATGAGAAA CTGCTAATAG ATATCATACA TTTTTTGAAT 
AAGTTAATAA AGGAACAAAG AAAAAACCCA TCAATTGAAC TTTTAAACTG GATTCTGGAA 
TTACTTCTTA GGCATAATCC AAGTCCACTA CTAGACCTGC TGGTTCTGAC AGATTCACAT 
GCAGGAGAAG AAACAGATGA CGTCCGGACT GCTGTCAGAC AGCAACTCCA GAAAGAACTG 
ATCAGTCTTC TCAATTCCTT GTTACTGGTT TTTATGACAG TCTCTGACAG GAAATGCTTG 
GAACTGTTTT ACGTTTTCCA AACACAGCTG TCTCTGAAGC TACTCCAGTG CCTCAGAGTC 
ACAGATGCTC CCCATTTCTA TGGCTTACCT TCATTGGAGA GGACCTTAGC AGGGATGGCT 
CACCTCACTG CCTTCCCTGG GTGGAGTGCA CACTCTCCTC TTTCAAAGCC ACTTGAGATT 
TGTGGGAAGT ACTTGTCCGG TCTCCTGGAG GTCATTACTT CCTTTTATGT TGAACGGGGA 
GGAAATGCTA TGTCCTTCAT GGGGAAAGGC GTTACAAAGA GCACAGGTCT TTGCTTACTT 
CACTTATCCC ATGAGATGAT AGTCCAGGCA CCAGGCTCGG AGTGGATGCC GCTTTGGTTC 
TTGCCTTTGG GTAGTCACAG TGAAGAACAT GCCCCTACTC AGCAAGGATT GGCCTGGCTA 
ATTCCCTTAT GGGTTGATAG AGACCCAGAG GTGAGATTCA CTTCACTGGG GTTGGGATCA 
GCACTGACTA CTATTGAAGC TGGCTGTGTG GCCTTGGCAA ACAGTTGTCA GAATATTTCT 
GGTGGGCTCT GGGGAACCGT GGTGAACATT CTTCTGGATC AATCAGAATG CAGTCTGGTG 
CGCCGGGAGG CTGCATTTAT TCTTCAGAAT CTCCTTGTAA TTCCAATGCC TTCAGAAATT 
ATAAAGGATT ATACTTGGCA GGGCCCCTGT GTCCATGATG AGGATTCTGG CCTGTCACTT 
TCAGGAAAAC CTGCCCTTCA GGCTCTTTTA TATCACTGTC GTTTCTATGA ACATTTGACT 
CACATGGTGA AACATTGTTA TGTAGGAAGA TATATCTTTG ATTTTAATTT TTCTGCTTTT 
ACTGAAACTT CAGAATACAG TGATTTAAAT GGTTTAGACG ACTCATTCAA GTTTTGGAGG 
GCCCCTTCTG GGACATCTAC TCAGGATCAT GAGCCAAGCT CTCTTTCCAC CTCAGAAACA 
ATGGTGGCTG CCTCGGTGGT CAGTTCTGAG ATTCAGCCCC TGCTGCCGTC AACACTTCTT 
CCTGAAACTG CCCTTGACCA GTTTGTGACG CAAGGTCAAC GTGAAATGAG ACCAGTGCGA 
CCTCGTGACT CATTGCTGTC TACTTCCCTG AACAGGCAGT ATGTGTTTGT TACTCCGTCT 
CTGCTGTCAG CAGTCTGTAG CCTTTTAGAC AACCTCTTGG CAGTTACTCC AAGAGATGCT 
GCAAATGCTT TTCAACAAGC TCATCTCATA GAACTCCTCT GTAGCACGGT GGATGCTTCC 
CTCATAGAAA TGTGCATCCA AGAACTCAAG ACCCCACTGC CTTTACCATC TGCTGTTGAA 
CATATTCAGG CTCAGGCTTC CTTTTTCTTG GAATACCTAT CCTCTTTGTC CCAGCTCTTG 
AAGTCCTGTC TGTTGGTAGA GCCTAAACTT GTGACACAAG ATGAGCTCTT GAAACCTCTT 
ATCACTAATG TCATCCGAGT TCTCATTGTG TGTCCCAAAG ATGTATTAGA TGTACAGTTA 
GTGTTGGCTT TTTACCGCAC ATGGACGCAT TTATTTGACC TTCTGGCCAC ACTACTGAGG 
AAAGCTGGTG CTGTCTCCCT TGTTCCTCTT ACCTCAGCTC TTGCCAAGCA TTGGGAGGCA 
GTGACTGATA CACTGTGTAG ATGTGTGGGC CTCTCATTCA AGTGCCCTGC CCTGACTATT 
GCCAGCCTCC AGTTCCTGTC TGTTCTCTTG GCTGAGGAAG AAAAGAGGCG TGTCCAGGAC 
AAGGACAAAA CAAATGAGGG CCAGGCCCCA ACAGTGGCCT TACTTCTTGA TGGAACTCAG 
GGAAGCCTTA GCTCTTCAGA GCGACTTAAT GAAACAATTC TTCAGTGCTA TGAAGGAATA 
TCCCCCAAAG ATGTCCTGAA AAGAGTTGCT GCAAATGCCC TGTTGTCACT TCTGGCTGTT 
AGCAGAAGAG CGCAGAGGCA TGCTTTGAGA TCTGATCTCA TAGAAAACTG TATGGAGCAG 
ATGAAGCACA TCAATGCACA ACTGAACTTA GATTTGCTGA GACCTGGGAA AGCAGTACTG 
AAAAAAAAGG AGGATGGCTT CATTAAAGAA TTAAGCATCA CCATGCAGCT CCTAAGAAAC 
TGCCTTTATC AAAATGAAGA ATGTAAAGTG GCAGCTCTTG AAGCACGTCT TGTGCCTGTA 
TTGCACTCTC TCTGGCCTTG GCTTTTGATG GAAGATTCAT TGATGCAAAT TGCCTTGCAG 
TTGCTTTGTG TCTATACTGC AAATTTTCCA GCTGGCTGCA GTTCCCTTTG TTGGTCAAAT 
TATGGACAGT GTATTCAAAC TGCACACAGA GGCACCCCCA GTAGCTCCCT GATACTGTGT 
ATCCTGAAAC TGGCTTCTCA GATGCCTGCA GAGAACACCA CCATTCAGCA GTTGGTTTTT 
ATGTTTCTTT CAAACTTGGC CTTATCTCAT GACTGCAAGG GAGTAATTCA AAAGAGCAAC 
TTTCTACAGC ACTTCTTGTC CCTGACATTG CCTAAAGGAG GAAACCAACA TCTCAGCACT 
GTGGCCATTC TGTGGTTGAA GTTGCTCCTG AATATGTCAT TTGGAGAAGA TGGACAGCAA 
ATGATCCTGA GGCTAGATGG CTGCTTAGAC TTACTCATAG AGATGAGCAA GTACAAGCAC 
AAGAGCAGCC CTCACATGCC TCTGCTGATC TTTCATAACA TTTGCTTCAG TCCTGCTAAT 
AAGCCTAAAA TCCTGGCTAA TGAAAAAGTT GTTACTCTTC TGGCTGCCTG TTTAGAAAGT 
GAGAATCAAA CTGCCCAGAG GATTGGAGCA GCTTCACTTT GGGCTCTAAC TTACAATTAC 
CAAAAGGCAA AAGCAACTTT GAAAAATCCA TCAATAAAAA GAAAAGTAGA TGAAGCATAT 
TCCATAGCAA AGAGAACTCT CTCAAACTCA GAAGAAAACC CTCTGAATTC CTACTATTTG 
AAATGCCTTG AAAATCTAGT ACAACTGCTT AACTATTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245382.1
CDS	831..5732
Translation	MCHHAWLQDWKSAQASPLLQGESQKVFLHMLSHPLPQVKVETYQCCLEIVKECLGVQNVTKPVSSLCSGIHFLLHPKVLYEISAFGIQESKNEVNAAAKAILLYLLQGRLMMTALTWNKFIESLAPVIPVLQGYADTEDPLGNCILLLSKVSSEAGEGALPCTARLKSVLRLLLVKKPSVRSLALKLLAFHLTSEEGADKKRPFIDAGVLSRVTNLFIVKKPIDLKLDDRRELVIKLETVKKVCDIFTSDDVDLVLRKSAAEQLAVIMQDIKMHTVVKKLCLVEKIIGHLNEFVGQDGEVIECLIQPCLTLLRKVLYADPVIRVSLSQQASLLTLLLRVSLIFHEDCTVVAEVGALFCLLLFDEVSRMDTWSDTLSDKPSLSSVFSLPVSVFRRYHLPVHVTGHHAVSPYSIVLPLPTECLTLKPVSDMLRIAWNLAWYHGSDNLLKQMTSEAKIQEFLDTLQLSTEDILALKITYTDSGLKDCLQSIIQAGGHRDVRAAITRMSFYLLNDKLSLKDDIGPCGVTLKSLAWHTVLNRFLQVLPACTEDEKLLIDIIHFLNKLIKEQRKNPSIELLNWILELLLRHNPSPLLDLLVLTDSHAGEETDDVRTAVRQQLQKELISLLNSLLLVFMTVSDRKCLELFYVFQTQLSLKLLQCLRVTDAPHFYGLPSLERTLAGMAHLTAFPGWSAHSPLSKPLEICGKYLSGLLEVITSFYVERGGNAMSFMGKGVTKSTGLCLLHLSHEMIVQAPGSEWMPLWFLPLGSHSEEHAPTQQGLAWLIPLWVDRDPEVRFTSLGLGSALTTIEAGCVALANSCQNISGGLWGTVVNILLDQSECSLVRREAAFILQNLLVIPMPSEIIKDYTWQGPCVHDEDSGLSLSGKPALQALLYHCRFYEHLTHMVKHCYVGRYIFDFNFSAFTETSEYSDLNGLDDSFKFWRAPSGTSTQDHEPSSLSTSETMVAASVVSSEIQPLLPSTLLPETALDQFVTQGQREMRPVRPRDSLLSTSLNRQYVFVTPSLLSAVCSLLDNLLAVTPRDAANAFQQAHLIELLCSTVDASLIEMCIQELKTPLPLPSAVEHIQAQASFFLEYLSSLSQLLKSCLLVEPKLVTQDELLKPLITNVIRVLIVCPKDVLDVQLVLAFYRTWTHLFDLLATLLRKAGAVSLVPLTSALAKHWEAVTDTLCRCVGLSFKCPALTIASLQFLSVLLAEEEKRRVQDKDKTNEGQAPTVALLLDGTQGSLSSSERLNETILQCYEGISPKDVLKRVAANALLSLLAVSRRAQRHALRSDLIENCMEQMKHINAQLNLDLLRPGKAVLKKKEDGFIKELSITMQLLRNCLYQNEECKVAALEARLVPVLHSLWPWLLMEDSLMQIALQLLCVYTANFPAGCSSLCWSNYGQCIQTAHRGTPSSSLILCILKLASQMPAENTTIQQLVFMFLSNLALSHDCKGVIQKSNFLQHFLSLTLPKGGNQHLSTVAILWLKLLLNMSFGEDGQQMILRLDGCLDLLIEMSKYKHKSSPHMPLLIFHNICFSPANKPKILANEKVVTLLAACLESENQTAQRIGAASLWALTYNYQKAKATLKNPSIKRKVDEAYSIAKRTLSNSEENPLNSYYLKCLENLVQLLNYS

Target ORF information:

RefSeq Version	XM_011247080.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus rotatin (Rttn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247080.2

ORF Insert Sequence:

ATGTGTCACC ATGCCTGGCT GCAAGACTGG AAATCTGCAC AGGCTAGTCC ATTACTCCAG 
GGAGAGAGTC AGAAGGTGTT TCTCCATATG CTGTCTCACC CATTGCCACA GGTGAAGGTG 
GAGACTTACC AGTGCTGCCT GGAGATTGTA AAGGAATGTT TAGGTGTCCA AAATGTCACT 
AAACCTGTGT CTTCACTTTG TAGTGGAATT CATTTTCTGC TTCATCCAAA AGTTCTGTAT 
GAAATCTCTG CATTTGGTAT TCAAGAATCC AAAAATGAGG TGAATGCTGC TGCCAAGGCC 
ATTCTGCTGT ATCTGCTTCA GGGACGATTG ATGATGACAG CTCTGACCTG GAACAAGTTT 
ATCGAATCTC TCGCTCCTGT CATTCCAGTG CTACAGGGCT ACGCTGATAC AGAAGATCCC 
TTAGGCAACT GCATTCTCCT TCTAAGCAAA GTGAGCTCTG AGGCAGGAGA AGGCGCTCTC 
CCCTGCACTG CCCGGCTGAA GTCTGTGCTA CGGCTTTTGC TAGTAAAAAA ACCTTCGGTT 
CGTTCACTAG CATTAAAACT TTTAGCATTT CATCTTACCA GTGAAGAAGG GGCTGATAAA 
AAGCGCCCGT TCATAGATGC TGGCGTTCTC TCCAGAGTTA CTAATCTGTT TATTGTGAAA 
AAACCTATTG ACCTTAAGTT GGATGACAGA AGAGAACTAG TTATTAAGTT GGAGACTGTT 
AAAAAGGTGT GTGACATATT CACCTCAGAC GATGTTGATC TTGTTTTAAG AAAGTCAGCT 
GCAGAGCAAC TAGCTGTGAT AATGCAAGAT ATTAAAATGC ATACAGTGGT GAAAAAGTTA 
TGCTTAGTTG AGAAGATTAT TGGACATTTA AATGAATTTG TGGGTCAAGA TGGTGAGGTT 
ATAGAATGCT TGATTCAGCC ATGCCTCACA CTCCTGAGGA AGGTTTTGTA CGCTGACCCG 
GTTATCCGTG TTTCCTTGTC ACAGCAGGCC TCTCTCTTGA CCCTATTACT CAGAGTTTCC 
TTGATATTTC ATGAAGATTG TACTGTGGTG GCTGAAGTCG GGGCATTGTT TTGTCTTCTA 
TTGTTCGATG AAGTATCAAG AATGGATACA TGGTCTGATA CTCTTTCTGA TAAGCCTTCT 
TTGTCATCAG TCTTCAGTTT GCCGGTTTCA GTCTTTAGAA GGTATCATCT ACCAGTTCAT 
GTGACTGGCC ACCATGCTGT GAGTCCTTAC TCAATAGTTT TGCCATTACC TACTGAATGT 
TTGACCCTGA AGCCGGTGTC AGATATGCTG AGAATAGCTT GGAACCTGGC ATGGTATCAT 
GGGAGTGATA ATCTATTGAA ACAAATGACC TCTGAAGCTA AAATTCAGGA ATTTTTAGAT 
ACACTGCAGC TGTCCACAGA AGATATCCTT GCACTGAAGA TCACATACAC GGATAGTGGG 
TTGAAGGACT GCCTTCAGTC CATCATTCAG GCTGGAGGCC ACAGGGATGT GAGGGCTGCT 
ATCACCAGGA TGAGTTTTTA TCTTTTGAAT GACAAACTGT CTTTGAAGGA TGATATTGGT 
CCATGTGGCG TTACTTTAAA ATCCTTGGCT TGGCACACTG TGCTGAACAG GTTTTTACAA 
GTACTTCCTG CTTGCACTGA AGATGAGAAA CTGCTAATAG ATATCATACA TTTTTTGAAT 
AAGTTAATAA AGGAACAAAG AAAAAACCCA TCAATTGAAC TTTTAAACTG GATTCTGGAA 
TTACTTCTTA GGCATAATCC AAGTCCACTA CTAGACCTGC TGGTTCTGAC AGATTCACAT 
GCAGGAGAAG AAACAGATGA CGTCCGGACT GCTGTCAGAC AGCAACTCCA GAAAGAACTG 
ATCAGTCTTC TCAATTCCTT GTTACTGGTT TTTATGACAG TCTCTGACAG GAAATGCTTG 
GAACTGTTTT ACGTTTTCCA AACACAGCTG TCTCTGAAGC TACTCCAGTG CCTCAGAGTC 
ACAGATGCTC CCCATTTCTA TGGCTTACCT TCATTGGAGA GGACCTTAGC AGGGATGGCT 
CACCTCACTG CCTTCCCTGG GTGGAGTGCA CACTCTCCTC TTTCAAAGCC ACTTGAGATT 
TGTGGGAAGT ACTTGTCCGG TCTCCTGGAG GTCATTACTT CCTTTTATGT TGAACGGGGA 
GGAAATGCTA TGTCCTTCAT GGGGAAAGGC GTTACAAAGA GCACAGGTCT TTGCTTACTT 
CACTTATCCC ATGAGATGAT AGTCCAGGCA CCAGGCTCGG AGTGGATGCC GCTTTGGTTC 
TTGCCTTTGG GTAGTCACAG TGAAGAACAT GCCCCTACTC AGCAAGGATT GGCCTGGCTA 
ATTCCCTTAT GGGTTGATAG AGACCCAGAG GTGAGATTCA CTTCACTGGG GTTGGGATCA 
GCACTGACTA CTATTGAAGC TGGCTGTGTG GCCTTGGCAA ACAGTTGTCA GAATATTTCT 
GGTGGGCTCT GGGGAACCGT GGTGAACATT CTTCTGGATC AATCAGAATG CAGTCTGGTG 
CGCCGGGAGG CTGCATTTAT TCTTCAGAAT CTCCTTGTAA TTCCAATGCC TTCAGAAATT 
ATAAAGGATT ATACTTGGCA GGGCCCCTGT GTCCATGATG AGGATTCTGG CCTGTCACTT 
TCAGGAAAAC CTGCCCTTCA GGCTCTTTTA TATCACTGTC GTTTCTATGA ACATTTGACT 
CACATGGTGA AACATTGTTA TGTAGGAAGA TATATCTTTG ATTTTAATTT TTCTGCTTTT 
ACTGAAACTT CAGAATACAG TGATTTAAAT GGTTTAGACG ACTCATTCAA GTTTTGGAGG 
GCCCCTTCTG GGACATCTAC TCAGGATCAT GAGCCAAGCT CTCTTTCCAC CTCAGAAACA 
ATGGTGGCTG CCTCGGTGGT CAGTTCTGAG ATTCAGCCCC TGCTGCCGTC AACACTTCTT 
CCTGAAACTG CCCTTGACCA GTTTGTGACG CAAGGTCAAC GTGAAATGAG ACCAGTGCGA 
CCTCGTGACT CATTGCTGTC TACTTCCCTG AACAGGCAGT ATGTGTTTGT TACTCCGTCT 
CTGCTGTCAG CAGTCTGTAG CCTTTTAGAC AACCTCTTGG CAGTTACTCC AAGAGATGCT 
GCAAATGCTT TTCAACAAGC TCATCTCATA GAACTCCTCT GTAGCACGGT GGATGCTTCC 
CTCATAGAAA TGTGCATCCA AGAACTCAAG ACCCCACTGC CTTTACCATC TGCTGTTGAA 
CATATTCAGG CTCAGGCTTC CTTTTTCTTG GAATACCTAT CCTCTTTGTC CCAGCTCTTG 
AAGTCCTGTC TGTTGGTAGA GCCTAAACTT GTGACACAAG ATGAGCTCTT GAAACCTCTT 
ATCACTAATG TCATCCGAGT TCTCATTGTG TGTCCCAAAG ATGTATTAGA TGTACAGTTA 
GTGTTGGCTT TTTACCGCAC ATGGACGCAT TTATTTGACC TTCTGGCCAC ACTACTGAGG 
AAAGCTGGTG CTGTCTCCCT TGTTCCTCTT ACCTCAGCTC TTGCCAAGCA TTGGGAGGCA 
GTGACTGATA CACTGTGTAG ATGTGTGGGC CTCTCATTCA AGTGCCCTGC CCTGACTATT 
GCCAGCCTCC AGTTCCTGTC TGTTCTCTTG GCTGAGGAAG AAAAGAGGCG TGTCCAGGAC 
AAGGACAAAA CAAATGAGGG CCAGGCCCCA ACAGTGGCCT TACTTCTTGA TGGAACTCAG 
GGAAGCCTTA GCTCTTCAGA GCGACTTAAT GAAACAATTC TTCAGTGCTA TGAAGGAATA 
TCCCCCAAAG ATGTCCTGAA AAGAGTTGCT GCAAATGCCC TGTTGTCACT TCTGGCTGTT 
AGCAGAAGAG CGCAGAGGCA TGCTTTGAGA TCTGATCTCA TAGAAAACTG TATGGAGCAG 
ATGAAGCACA TCAATGCACA ACTGAACTTA GATTTGCTGA GACCTGGGAA AGCAGTACTG 
AAAAAAAAGG AGGATGGCTT CATTAAAGAA TTAAGCATCA CCATGCAGCT CCTAAGAAAC 
TGCCTTTATC AAAATGAAGA ATGTAAAGTG GCAGCTCTTG AAGCACGTCT TGTGCCTGTA 
TTGCACTCTC TCTGGCCTTG GCTTTTGATG GAAGATTCAT TGATGCAAAT TGCCTTGCAG 
TTGCTTTGTG TCTATACTGC AAATTTTCCA GCTGGCTGCA GTTCCCTTTG TTGGTCAAAT 
TATGGACAGT GTATTCAAAC TGCACACAGA GGCACCCCCA GTAGCTCCCT GATACTGTGT 
ATCCTGAAAC TGGCTTCTCA GATGCCTGCA GAGAACACCA CCATTCAGCA GTTGGTTTTT 
ATGTTTCTTT CAAACTTGGC CTTATCTCAT GACTGCAAGG GAGTAATTCA AAAGAGCAAC 
TTTCTACAGC ACTTCTTGTC CCTGACATTG CCTAAAGGAG GAAACCAACA TCTCAGCACT 
GTGGCCATTC TGTGGTTGAA GTTGCTCCTG AATATGTCAT TTGGAGAAGA TGGACAGCAA 
ATGATCCTGA GGCTAGATGG CTGCTTAGAC TTACTCATAG AGATGAGCAA GTACAAGCAC 
AAGAGCAGCC CTCACATGCC TCTGCTGATC TTTCATAACA TTTGCTTCAG TCCTGCTAAT 
AAGCCTAAAA TCCTGGCTAA TGAAAAAGTT GTTACTCTTC TGGCTGCCTG TTTAGAAAGT 
GAGAATCAAA CTGCCCAGAG GATTGGAGCA GCTTCACTTT GGGCTCTAAC TTACAATTAC 
CAAAAGGCAA AAGCAACTTT GAAAAATCCA TCAATAAAAA GAAAAGTAGA TGAAGCATAT 
TCCATAGCAA AGAGAACTCT CTCAAACTCA GAAGAAAACC CTCTGAATTC CTACTATTTG 
AAATGCCTTG AAAATCTAGT ACAACTGCTT AACTATTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rttn ( NM_175542.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_780751.2 Mus musculus rotatin (Rttn), mRNA.

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CloneID	OMu05549
Clone ID Related Accession (Same CDS sequence)	NM_175542.3
Accession Version	NM_175542.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Mus musculus(house mouse)
Product	rotatin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF265232.1 and BC023916.1. On Oct 17, 2003 this sequence version replaced NM_175542.2. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTTCTGG CGGGGCTCAT CAGGAAGCTC GGCCATCAGC TGGCGGAGAT CAGGGAACGC 
GCCCTCAAAA GTATCCTGTG CAAGATTGAG CACAACTTGA TCTGCTATGC TGACTTGATT 
CAGGAAAGGT TGCTTTTTCT CCATTTGCTC GAATGGTTCA ATTTCCCATC TGTCCCAATG 
AAAGAGGAGG TTCTGAACTT GTTAAGCAGA TTGGTTAAGT ATTCTCCAGC CGTCCAACAC 
TTGATTGACC TTGGTGCAGT AGAGTTTTTA TCTAAGCTCC GTCCAAATGT GGAGCCAGTA 
ATACAGGCTG AGATTGATGG CATTCTCGAT GGACTTTTCA TTCTTCCTTC AGAAGTTCCT 
GCACTGTGTT CTTCCAGTTA CCACACTGAT CAAATTGAAT TGTCACAACA ACCTGAAGTC 
TTAACAGGAT ATTTTCCCCA AGACAAAAGT AATTTCCAGC AGATGGAAGT ACCACCAGGG 
CCAGCGGGAA ACCAGGCTGT GAAGTGGCTG AGGTTCTCTG TGTTTCCTTG GCTACCCCTG 
ACCACAACAG ACAGACATGT TCTCTCTTCC AATGAAAGCT CTTTGAGAAG TAGTAACCAC 
ACTCTGATCT GGAACACCTG TGAGCTTCTG AAGGACGTGA TCATGCAAGA CTTTCCTGCT 
GAGATTTTTC TTCAGAGGCC AAAGATTGTT CAGGGCCTTT TGTGTCTGGT GAAGCTGGCC 
TTCGGAGGAG ATGGGAAGCA TCGTCTAGCA TTACAGTCAG TGTCCTGTTT GCAGCAGCTG 
TGCACAAGCT TAAGAAACAG ACTCAACTTT TACAGAGACC CCAACTTTTT CTCCAGTAGA 
CAAGACACAG TCTCCCAGAA TTCTTCTTTG TCTTACTGTC ATGAAGCAAG AGGTCCTTAC 
CATTCCCCAA ACCCATCCCC GGGAAGCAGT AGCTCTCGGC CTTCTGTGGT TGGGCGCACA 
GGCCAGCGAC CCCGAGGAGA TGGTCAGGAC TGGGATGCAG TGTCTTCCAG TGGAAGTAGT 
AGTCACACGC ATGTGAACTC TAGGATATCT GTGCATTCTC CTTTGGACGT GGCACCCGTG 
GATCTGCCGG AGCTGGAAGC AGAGGACACA CTGGAGCTGC AGTTCCAACA GCTTAGTCTT 
CCTCAGTTCT GCATCTCCAC CCTGGAGGCA GCAGCTCCTC TGCTGAGAAC AGGCAGCAGA 
GAAATGATAA TAAGAGTTCT GGAACTGCTT GCAGAGGACA TGCTGCTCAT TGGACAAGCA 
ATTTCATCAG AAATCTGGGA TGATAACAGC CTTTTTGCTA TCAATGTGAA AGAGAAACTG 
CTCCAAGTGT TGGGGTCCCT TGGAGAAACC ATGTGCTACC GTAAAACCAG TTTTAGCATG 
GAGCAAGCCG AGGCTGTACT CGTGCACCAC AGAATGGCCT TTGTCAGCAT CTCACTGTTC 
ACAGTTCGAC TGTTGCAACT TCTTCTCCCT GTTGAAAAGG CGAGTGAGTT TTTGCCAGAG 
TCCATGTCAG CAGCATTATT TTTTGTCTCC TTGGACATGC CTATTTCTTT GGAATATCCA 
GAGATTCATG ACACAGTTGT GGCTTATTTG GAACAGCTGG ATTCTGAAAA TTACAGCATT 
TATAAGCGAA CTGCAGAGGC TGTTTATTCA ATTGAATGCA CCTGTAACTT CCTATCTGAT 
GTGGGGAAAG AGGGAGAAAA GAATCTTCTA GAATTAGTGG AGCTGGCAGA CCAAGCCTTG 
CGTAGCTTTT CCTATCATCA GCATTCTCCC CTAATAAAGG AAATCATCTG CATCTGTTCA 
AAGATCTGGA AATCTGCACA GGCTAGTCCA TTACTCCAGG GAGAGAGTCA GAAGGTGTTT 
CTCCATATGC TGTCTCACCC ATTGCCACAG GTGAAGGTGG AGACTTACCA GTGCTGCCTG 
GAGATTGTAA AGGAATGTTT AGGTGTCCAA AATGTCACTA AACCTGTGTC TTCACTTTGT 
AGTGGAATTC ATTTTCTGCT TCATCCAAAA GTTCTGTATG AAATCTCTGC ATTTGGTATT 
CAAGAATCCA AAAATGAGGT GAATGCTGCT GCCAAGGCCA TTCTGCTGTA TCTGCTTCAG 
GGACGATTGA TGATGACAGC TCTGACCTGG AACAAGTTTA TCGAATCTCT CGCTCCTGTC 
ATTCCAGTGC TACAGGGCTA CGCTGATACA GAAGATCCCT TAGGCAACTG CATTCTCCTT 
CTAAGCAAAG TGAGCTCTGA GGCAGGAGAA GGCGCTCTCC CCTGCACTGC CCGGCTGAAG 
TCTGTGCTAC GGCTTTTGCT AGTAAAAAAA CCTTCGGTTC GTTCACTAGC ATTAAAACTT 
TTAGCATTTC ATCTTACCAG TGAAGAAGGG GCTGATAAAA AGCGCCCGTT CATAGATGCT 
GGCGTTCTCT CCAGAGTTAC TAATCTGTTT ATTGTGAAAA AACCTATTGA CCTTAAGTTG 
GATGACAGAA GAGAACTAGT TATTAAGTTG GAGACTGTTA AAAAGGTGTG TGACATATTC 
ACCTCAGACG ATGTTGATCT TGTTTTAAGA AAGTCAGCTG CAGAGCAACT AGCTGTGATA 
ATGCAAGATA TTAAAATGCA TACAGTGGTG AAAAAGTTAT GCTTAGTTGA GAAGATTATT 
GGACATTTAA ATGAATTTGT GGGTCAAGAT GGTGAGGTTA TAGAATGCTT GATTCAGCCA 
TGCCTCACAC TCCTGAGGAA GGTTTTGTAC GCTGACCCGG TTATCCGTGT TTCCTTGTCA 
CAGCAGGCCT CTCTCTTGAC CCTATTACTC AGAGTTTCCT TGATATTTCA TGAAGATTGT 
ACTGTGGTGG CTGAAGTCGG GGCATTGTTT TGTCTTCTAT TGTTCGATGA AGTATCAAGA 
ATGGATACAT GGTCTGATAC TCTTTCTGAT AAGCCTTCTT TGTCATCAGT CTTCAGTTTG 
CCGGTTTCAG TCTTTAGAAG GTATCATCTA CCAGTTCATG TGACTGGCCA CCATGCTGTG 
AGTCCTTACT CAATAGTTTT GCCATTACCT ACTGAATGTT TGACCCTGAA GCCGGTGTCA 
GATATGCTGA GAATAGCTTG GAACCTGGCA TGGTATCATG GGAGTGATAA TCTATTGAAA 
CAAATGACCT CTGAAGCTAA AATTCAGGAA TTTTTAGATA CACTGCAGCT GTCCACAGAA 
GATATCCTTG CACTGAAGAT CACATACACG GATAGTGGGT TGAAGGACTG CCTTCAGTCC 
ATCATTCAGG CTGGAGGCCA CAGGGATGTG AGGGCTGCTA TCACCAGGAT GAGTTTTTAT 
CTTTTGAATG ACAAACTGTC TTTGAAGGAT GATATTGGTC CATGTGGCGT TACTTTAAAA 
TCCTTGGCTT GGCACACTGT GCTGAACAGG TTTTTACAAG TACTTCCTGC TTGCACTGAA 
GATGAGAAAC TGCTAATAGA TATCATACAT TTTTTGAATA AGTTAATAAA GGAACAAAGA 
AAAAACCCAT CAATTGAACT TTTAAACTGG ATTCTGGAAT TACTTCTTAG GCATAATCCA 
AGTCCACTAC TAGACCTGCT GGTTCTGACA GATTCACATG CAGGAGAAGA AACAGATGAC 
GTCCGGACTG CTGTCAGACA GCAACTCCAG AAAGAACTGA TCAGTCTTCT CAATTCCTTG 
TTACTGGTTT TTATGACAGT CTCTGACAGG AAATGCTTGG AACTGTTTTA CGTTTTCCAA 
ACACAGCTGT CTCTGAAGCT ACTCCAGTGC CTCAGAGTCA CAGATGCTCC CCATTTCTAT 
GGCTTACCTT CATTGGAGAG GACCTTAGCA GGGATGGCTC ACCTCACTGC CTTCCCTGGG 
TGGAGTGCAC ACTCTCCTCT TTCAAAGCCA CTTGAGATTT GTGGGAAGTA CTTGTCCGGT 
CTCCTGGAGG TCATTACTTC CTTTTATGTT GAACGGGGAG GAAATGCTAT GTCCTTCATG 
GGGAAAGGCG TTACAAAGAG CACAGGTCTT TGCTTACTTC ACTTATCCCA TGAGATGATA 
GTCCAGGCAC CAGGCTCGGA GTGGATGCCG CTTTGGTTCT TGCCTTTGGG TAGTCACAGT 
GAAGAACATG CCCCTACTCA GCAAGGATTG GCCTGGCTAA TTCCCTTATG GGTTGATAGA 
GACCCAGAGG TGAGATTCAC TTCACTGGGG TTGGGATCAG CACTGACTAC TATTGAAGCT 
GGCTGTGTGG CCTTGGCAAA CAGTTGTCAG AATATTTCTG GTGGGCTCTG GGGAACCGTG 
GTGAACATTC TTCTGGATCA ATCAGAATGC AGTCTGGTGC GCCGGGAGGC TGCATTTATT 
CTTCAGAATC TCCTTGTAAT TCCAATGCCT TCAGAAATTA TAAAGGATTA TACTTGGCAG 
GGCCCCTGTG TCCATGATGA GGATTCTGGC CTGTCACTTT CAGGAAAACC TGCCCTTCAG 
GCTCTTTTAT ATCACTGTCG TTTCTATGAA CATTTGACTC ACATGGTGAA ACATTGTTAT 
GTAGGAAGAT ATATCTTTGA TTTTAATTTT TCTGCTTTTA CTGAAACTTC AGAATACAGT 
GATTTAAATG GTTTAGACGA CTCATTCAAG TTTTGGAGGG CCCCTTCTGG GACATCTACT 
CAGGATCATG AGCCAAGCTC TCTTTCCACC TCAGAAACAA TGGTGGCTGC CTCGGTGGTC 
AGTTCTGAGA TTCAGCCCCT GCTGCCGTCA ACACTTCTTC CTGAAACTGC CCTTGACCAG 
TTTGTGACGC AAGGTCAACG TGAAATGAGA CCAGTGCGAC CTCGTGACTC ATTGCTGTCT 
ACTTCCCTGA ACAGGCAGTA TGTGTTTGTT ACTCCGTCTC TGCTGTCAGC AGTCTGTAGC 
CTTTTAGACA ACCTCTTGGC AGTTACTCCA AGAGATGCTG CAAATGCTTT TCAACAAGCT 
CATCTCATAG AACTCCTCTG TAGCACGGTG GATGCTTCCC TCATAGAAAT GTGCATCCAA 
GAACTCAAGA CCCCACTGCC TTTACCATCT GCTGTTGAAC ATATTCAGGC TCAGGCTTCC 
TTTTTCTTGG AATACCTATC CTCTTTGTCC CAGCTCTTGA AGTCCTGTCT GTTGGTAGAG 
CCTAAACTTG TGACACAAGA TGAGCTCTTG AAACCTCTTA TCACTAATGT CATCCGAGTT 
CTCATTGTGT GTCCCAAAGA TGTATTAGAT GTACAGTTAG TGTTGGCTTT TTACCGCACA 
TGGACGCATT TATTTGACCT TCTGGCCACA CTACTGAGGA AAGCTGGTGC TGTCTCCCTT 
GTTCCTCTTA CCTCAGCTCT TGCCAAGCAT TGGGAGGCAG TGACTGATAC ACTGTGTAGA 
TGTGTGGGCC TCTCATTCAA GTGCCCTGCC CTGACTATTG CCAGCCTCCA GTTCCTGTCT 
GTTCTCTTGG CTGAGGAAGA AAAGAGGCGT GTCCAGGACA AGGACAAAAC AAATGAGGGC 
CAGGCCCCAA CAGTGGCCTT ACTTCTTGAT GGAACTCAGG GAAGCCTTAG CTCTTCAGAG 
CGACTTAATG AAACAATTCT TCAGTGCTAT GAAGGAATAT CCCCCAAAGA TGTCCTGAAA 
AGAGTTGCTG CAAATGCCCT GTTGTCACTT CTGGCTGTTA GCAGAAGAGC GCAGAGGCAT 
GCTTTGAGAT CTGATCTCAT AGAAAACTGT ATGGAGCAGA TGAAGCACAT CAATGCACAA 
CTGAACTTAG ATTTGCTGAG ACCTGGGAAA GCAGTACTGA AAAAAAAGGA GGATGGCTTC 
ATTAAAGAAT TAAGCATCAC CATGCAGCTC CTAAGAAACT GCCTTTATCA AAATGAAGAA 
TGTAAAGTGG CAGCTCTTGA AGCACGTCTT GTGCCTGTAT TGCACTCTCT CTGGCCTTGG 
CTTTTGATGG AAGATTCATT GATGCAAATT GCCTTGCAGT TGCTTTGTGT CTATACTGCA 
AATTTTCCAG CTGGCTGCAG TTCCCTTTGT TGGTCAAATT ATGGACAGTG TATTCAAACT 
GCACACAGAG GCACCCCCAG TAGCTCCCTG ATACTGTGTA TCCTGAAACT GGCTTCTCAG 
ATGCCTGCAG AGAACACCAC CATTCAGCAG TTGGTTTTTA TGTTTCTTTC AAACTTGGCC 
TTATCTCATG ACTGCAAGGG AGTAATTCAA AAGAGCAACT TTCTACAGCA CTTCTTGTCC 
CTGACATTGC CTAAAGGAGG AAACCAACAT CTCAGCACTG TGGCCATTCT GTGGTTGAAG 
TTGCTCCTGA ATATGTCATT TGGAGAAGAT GGACAGCAAA TGATCCTGAG GCTAGATGGC 
TGCTTAGACT TACTCATAGA GATGAGCAAG TACAAGCACA AGAGCAGCCC TCACATGCCT 
CTGCTGATCT TTCATAACAT TTGCTTCAGT CCTGCTAATA AGCCTAAAAT CCTGGCTAAT 
GAAAAAGTTG TTACTCTTCT GGCTGCCTGT TTAGAAAGTG AGAATCAAAC TGCCCAGAGG 
ATTGGAGCAG CTTCACTTTG GGCTCTAACT TACAATTACC AAAAGGCAAA AGCAACTTTG 
AAAAATCCAT CAATAAAAAG AAAAGTAGAT GAAGCATATT CCATAGCAAA GAGAACTCTC 
TCAAACTCAG AAGAAAACCC TCTGAATTCC TACTATTTGA AATGCCTTGA AAATCTAGTA 
CAACTGCTTA ACTATTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_780751.2
CDS	259..6939
Translation	MVLAGLIRKLGHQLAEIRERALKSILCKIEHNLICYADLIQERLLFLHLLEWFNFPSVPMKEEVLNLLSRLVKYSPAVQHLIDLGAVEFLSKLRPNVEPVIQAEIDGILDGLFILPSEVPALCSSSYHTDQIELSQQPEVLTGYFPQDKSNFQQMEVPPGPAGNQAVKWLRFSVFPWLPLTTTDRHVLSSNESSLRSSNHTLIWNTCELLKDVIMQDFPAEIFLQRPKIVQGLLCLVKLAFGGDGKHRLALQSVSCLQQLCTSLRNRLNFYRDPNFFSSRQDTVSQNSSLSYCHEARGPYHSPNPSPGSSSSRPSVVGRTGQRPRGDGQDWDAVSSSGSSSHTHVNSRISVHSPLDVAPVDLPELEAEDTLELQFQQLSLPQFCISTLEAAAPLLRTGSREMIIRVLELLAEDMLLIGQAISSEIWDDNSLFAINVKEKLLQVLGSLGETMCYRKTSFSMEQAEAVLVHHRMAFVSISLFTVRLLQLLLPVEKASEFLPESMSAALFFVSLDMPISLEYPEIHDTVVAYLEQLDSENYSIYKRTAEAVYSIECTCNFLSDVGKEGEKNLLELVELADQALRSFSYHQHSPLIKEIICICSKIWKSAQASPLLQGESQKVFLHMLSHPLPQVKVETYQCCLEIVKECLGVQNVTKPVSSLCSGIHFLLHPKVLYEISAFGIQESKNEVNAAAKAILLYLLQGRLMMTALTWNKFIESLAPVIPVLQGYADTEDPLGNCILLLSKVSSEAGEGALPCTARLKSVLRLLLVKKPSVRSLALKLLAFHLTSEEGADKKRPFIDAGVLSRVTNLFIVKKPIDLKLDDRRELVIKLETVKKVCDIFTSDDVDLVLRKSAAEQLAVIMQDIKMHTVVKKLCLVEKIIGHLNEFVGQDGEVIECLIQPCLTLLRKVLYADPVIRVSLSQQASLLTLLLRVSLIFHEDCTVVAEVGALFCLLLFDEVSRMDTWSDTLSDKPSLSSVFSLPVSVFRRYHLPVHVTGHHAVSPYSIVLPLPTECLTLKPVSDMLRIAWNLAWYHGSDNLLKQMTSEAKIQEFLDTLQLSTEDILALKITYTDSGLKDCLQSIIQAGGHRDVRAAITRMSFYLLNDKLSLKDDIGPCGVTLKSLAWHTVLNRFLQVLPACTEDEKLLIDIIHFLNKLIKEQRKNPSIELLNWILELLLRHNPSPLLDLLVLTDSHAGEETDDVRTAVRQQLQKELISLLNSLLLVFMTVSDRKCLELFYVFQTQLSLKLLQCLRVTDAPHFYGLPSLERTLAGMAHLTAFPGWSAHSPLSKPLEICGKYLSGLLEVITSFYVERGGNAMSFMGKGVTKSTGLCLLHLSHEMIVQAPGSEWMPLWFLPLGSHSEEHAPTQQGLAWLIPLWVDRDPEVRFTSLGLGSALTTIEAGCVALANSCQNISGGLWGTVVNILLDQSECSLVRREAAFILQNLLVIPMPSEIIKDYTWQGPCVHDEDSGLSLSGKPALQALLYHCRFYEHLTHMVKHCYVGRYIFDFNFSAFTETSEYSDLNGLDDSFKFWRAPSGTSTQDHEPSSLSTSETMVAASVVSSEIQPLLPSTLLPETALDQFVTQGQREMRPVRPRDSLLSTSLNRQYVFVTPSLLSAVCSLLDNLLAVTPRDAANAFQQAHLIELLCSTVDASLIEMCIQELKTPLPLPSAVEHIQAQASFFLEYLSSLSQLLKSCLLVEPKLVTQDELLKPLITNVIRVLIVCPKDVLDVQLVLAFYRTWTHLFDLLATLLRKAGAVSLVPLTSALAKHWEAVTDTLCRCVGLSFKCPALTIASLQFLSVLLAEEEKRRVQDKDKTNEGQAPTVALLLDGTQGSLSSSERLNETILQCYEGISPKDVLKRVAANALLSLLAVSRRAQRHALRSDLIENCMEQMKHINAQLNLDLLRPGKAVLKKKEDGFIKELSITMQLLRNCLYQNEECKVAALEARLVPVLHSLWPWLLMEDSLMQIALQLLCVYTANFPAGCSSLCWSNYGQCIQTAHRGTPSSSLILCILKLASQMPAENTTIQQLVFMFLSNLALSHDCKGVIQKSNFLQHFLSLTLPKGGNQHLSTVAILWLKLLLNMSFGEDGQQMILRLDGCLDLLIEMSKYKHKSSPHMPLLIFHNICFSPANKPKILANEKVVTLLAACLESENQTAQRIGAASLWALTYNYQKAKATLKNPSIKRKVDEAYSIAKRTLSNSEENPLNSYYLKCLENLVQLLNYS

Target ORF information:

RefSeq Version	NM_175542.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus rotatin (Rttn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175542.3

ORF Insert Sequence:

ATGGTTCTGG CGGGGCTCAT CAGGAAGCTC GGCCATCAGC TGGCGGAGAT CAGGGAACGC 
GCCCTCAAAA GTATCCTGTG CAAGATTGAG CACAACTTGA TCTGCTATGC TGACTTGATT 
CAGGAAAGGT TGCTTTTTCT CCATTTGCTC GAATGGTTCA ATTTCCCATC TGTCCCAATG 
AAAGAGGAGG TTCTGAACTT GTTAAGCAGA TTGGTTAAGT ATTCTCCAGC CGTCCAACAC 
TTGATTGACC TTGGTGCAGT AGAGTTTTTA TCTAAGCTCC GTCCAAATGT GGAGCCAGTA 
ATACAGGCTG AGATTGATGG CATTCTCGAT GGACTTTTCA TTCTTCCTTC AGAAGTTCCT 
GCACTGTGTT CTTCCAGTTA CCACACTGAT CAAATTGAAT TGTCACAACA ACCTGAAGTC 
TTAACAGGAT ATTTTCCCCA AGACAAAAGT AATTTCCAGC AGATGGAAGT ACCACCAGGG 
CCAGCGGGAA ACCAGGCTGT GAAGTGGCTG AGGTTCTCTG TGTTTCCTTG GCTACCCCTG 
ACCACAACAG ACAGACATGT TCTCTCTTCC AATGAAAGCT CTTTGAGAAG TAGTAACCAC 
ACTCTGATCT GGAACACCTG TGAGCTTCTG AAGGACGTGA TCATGCAAGA CTTTCCTGCT 
GAGATTTTTC TTCAGAGGCC AAAGATTGTT CAGGGCCTTT TGTGTCTGGT GAAGCTGGCC 
TTCGGAGGAG ATGGGAAGCA TCGTCTAGCA TTACAGTCAG TGTCCTGTTT GCAGCAGCTG 
TGCACAAGCT TAAGAAACAG ACTCAACTTT TACAGAGACC CCAACTTTTT CTCCAGTAGA 
CAAGACACAG TCTCCCAGAA TTCTTCTTTG TCTTACTGTC ATGAAGCAAG AGGTCCTTAC 
CATTCCCCAA ACCCATCCCC GGGAAGCAGT AGCTCTCGGC CTTCTGTGGT TGGGCGCACA 
GGCCAGCGAC CCCGAGGAGA TGGTCAGGAC TGGGATGCAG TGTCTTCCAG TGGAAGTAGT 
AGTCACACGC ATGTGAACTC TAGGATATCT GTGCATTCTC CTTTGGACGT GGCACCCGTG 
GATCTGCCGG AGCTGGAAGC AGAGGACACA CTGGAGCTGC AGTTCCAACA GCTTAGTCTT 
CCTCAGTTCT GCATCTCCAC CCTGGAGGCA GCAGCTCCTC TGCTGAGAAC AGGCAGCAGA 
GAAATGATAA TAAGAGTTCT GGAACTGCTT GCAGAGGACA TGCTGCTCAT TGGACAAGCA 
ATTTCATCAG AAATCTGGGA TGATAACAGC CTTTTTGCTA TCAATGTGAA AGAGAAACTG 
CTCCAAGTGT TGGGGTCCCT TGGAGAAACC ATGTGCTACC GTAAAACCAG TTTTAGCATG 
GAGCAAGCCG AGGCTGTACT CGTGCACCAC AGAATGGCCT TTGTCAGCAT CTCACTGTTC 
ACAGTTCGAC TGTTGCAACT TCTTCTCCCT GTTGAAAAGG CGAGTGAGTT TTTGCCAGAG 
TCCATGTCAG CAGCATTATT TTTTGTCTCC TTGGACATGC CTATTTCTTT GGAATATCCA 
GAGATTCATG ACACAGTTGT GGCTTATTTG GAACAGCTGG ATTCTGAAAA TTACAGCATT 
TATAAGCGAA CTGCAGAGGC TGTTTATTCA ATTGAATGCA CCTGTAACTT CCTATCTGAT 
GTGGGGAAAG AGGGAGAAAA GAATCTTCTA GAATTAGTGG AGCTGGCAGA CCAAGCCTTG 
CGTAGCTTTT CCTATCATCA GCATTCTCCC CTAATAAAGG AAATCATCTG CATCTGTTCA 
AAGATCTGGA AATCTGCACA GGCTAGTCCA TTACTCCAGG GAGAGAGTCA GAAGGTGTTT 
CTCCATATGC TGTCTCACCC ATTGCCACAG GTGAAGGTGG AGACTTACCA GTGCTGCCTG 
GAGATTGTAA AGGAATGTTT AGGTGTCCAA AATGTCACTA AACCTGTGTC TTCACTTTGT 
AGTGGAATTC ATTTTCTGCT TCATCCAAAA GTTCTGTATG AAATCTCTGC ATTTGGTATT 
CAAGAATCCA AAAATGAGGT GAATGCTGCT GCCAAGGCCA TTCTGCTGTA TCTGCTTCAG 
GGACGATTGA TGATGACAGC TCTGACCTGG AACAAGTTTA TCGAATCTCT CGCTCCTGTC 
ATTCCAGTGC TACAGGGCTA CGCTGATACA GAAGATCCCT TAGGCAACTG CATTCTCCTT 
CTAAGCAAAG TGAGCTCTGA GGCAGGAGAA GGCGCTCTCC CCTGCACTGC CCGGCTGAAG 
TCTGTGCTAC GGCTTTTGCT AGTAAAAAAA CCTTCGGTTC GTTCACTAGC ATTAAAACTT 
TTAGCATTTC ATCTTACCAG TGAAGAAGGG GCTGATAAAA AGCGCCCGTT CATAGATGCT 
GGCGTTCTCT CCAGAGTTAC TAATCTGTTT ATTGTGAAAA AACCTATTGA CCTTAAGTTG 
GATGACAGAA GAGAACTAGT TATTAAGTTG GAGACTGTTA AAAAGGTGTG TGACATATTC 
ACCTCAGACG ATGTTGATCT TGTTTTAAGA AAGTCAGCTG CAGAGCAACT AGCTGTGATA 
ATGCAAGATA TTAAAATGCA TACAGTGGTG AAAAAGTTAT GCTTAGTTGA GAAGATTATT 
GGACATTTAA ATGAATTTGT GGGTCAAGAT GGTGAGGTTA TAGAATGCTT GATTCAGCCA 
TGCCTCACAC TCCTGAGGAA GGTTTTGTAC GCTGACCCGG TTATCCGTGT TTCCTTGTCA 
CAGCAGGCCT CTCTCTTGAC CCTATTACTC AGAGTTTCCT TGATATTTCA TGAAGATTGT 
ACTGTGGTGG CTGAAGTCGG GGCATTGTTT TGTCTTCTAT TGTTCGATGA AGTATCAAGA 
ATGGATACAT GGTCTGATAC TCTTTCTGAT AAGCCTTCTT TGTCATCAGT CTTCAGTTTG 
CCGGTTTCAG TCTTTAGAAG GTATCATCTA CCAGTTCATG TGACTGGCCA CCATGCTGTG 
AGTCCTTACT CAATAGTTTT GCCATTACCT ACTGAATGTT TGACCCTGAA GCCGGTGTCA 
GATATGCTGA GAATAGCTTG GAACCTGGCA TGGTATCATG GGAGTGATAA TCTATTGAAA 
CAAATGACCT CTGAAGCTAA AATTCAGGAA TTTTTAGATA CACTGCAGCT GTCCACAGAA 
GATATCCTTG CACTGAAGAT CACATACACG GATAGTGGGT TGAAGGACTG CCTTCAGTCC 
ATCATTCAGG CTGGAGGCCA CAGGGATGTG AGGGCTGCTA TCACCAGGAT GAGTTTTTAT 
CTTTTGAATG ACAAACTGTC TTTGAAGGAT GATATTGGTC CATGTGGCGT TACTTTAAAA 
TCCTTGGCTT GGCACACTGT GCTGAACAGG TTTTTACAAG TACTTCCTGC TTGCACTGAA 
GATGAGAAAC TGCTAATAGA TATCATACAT TTTTTGAATA AGTTAATAAA GGAACAAAGA 
AAAAACCCAT CAATTGAACT TTTAAACTGG ATTCTGGAAT TACTTCTTAG GCATAATCCA 
AGTCCACTAC TAGACCTGCT GGTTCTGACA GATTCACATG CAGGAGAAGA AACAGATGAC 
GTCCGGACTG CTGTCAGACA GCAACTCCAG AAAGAACTGA TCAGTCTTCT CAATTCCTTG 
TTACTGGTTT TTATGACAGT CTCTGACAGG AAATGCTTGG AACTGTTTTA CGTTTTCCAA 
ACACAGCTGT CTCTGAAGCT ACTCCAGTGC CTCAGAGTCA CAGATGCTCC CCATTTCTAT 
GGCTTACCTT CATTGGAGAG GACCTTAGCA GGGATGGCTC ACCTCACTGC CTTCCCTGGG 
TGGAGTGCAC ACTCTCCTCT TTCAAAGCCA CTTGAGATTT GTGGGAAGTA CTTGTCCGGT 
CTCCTGGAGG TCATTACTTC CTTTTATGTT GAACGGGGAG GAAATGCTAT GTCCTTCATG 
GGGAAAGGCG TTACAAAGAG CACAGGTCTT TGCTTACTTC ACTTATCCCA TGAGATGATA 
GTCCAGGCAC CAGGCTCGGA GTGGATGCCG CTTTGGTTCT TGCCTTTGGG TAGTCACAGT 
GAAGAACATG CCCCTACTCA GCAAGGATTG GCCTGGCTAA TTCCCTTATG GGTTGATAGA 
GACCCAGAGG TGAGATTCAC TTCACTGGGG TTGGGATCAG CACTGACTAC TATTGAAGCT 
GGCTGTGTGG CCTTGGCAAA CAGTTGTCAG AATATTTCTG GTGGGCTCTG GGGAACCGTG 
GTGAACATTC TTCTGGATCA ATCAGAATGC AGTCTGGTGC GCCGGGAGGC TGCATTTATT 
CTTCAGAATC TCCTTGTAAT TCCAATGCCT TCAGAAATTA TAAAGGATTA TACTTGGCAG 
GGCCCCTGTG TCCATGATGA GGATTCTGGC CTGTCACTTT CAGGAAAACC TGCCCTTCAG 
GCTCTTTTAT ATCACTGTCG TTTCTATGAA CATTTGACTC ACATGGTGAA ACATTGTTAT 
GTAGGAAGAT ATATCTTTGA TTTTAATTTT TCTGCTTTTA CTGAAACTTC AGAATACAGT 
GATTTAAATG GTTTAGACGA CTCATTCAAG TTTTGGAGGG CCCCTTCTGG GACATCTACT 
CAGGATCATG AGCCAAGCTC TCTTTCCACC TCAGAAACAA TGGTGGCTGC CTCGGTGGTC 
AGTTCTGAGA TTCAGCCCCT GCTGCCGTCA ACACTTCTTC CTGAAACTGC CCTTGACCAG 
TTTGTGACGC AAGGTCAACG TGAAATGAGA CCAGTGCGAC CTCGTGACTC ATTGCTGTCT 
ACTTCCCTGA ACAGGCAGTA TGTGTTTGTT ACTCCGTCTC TGCTGTCAGC AGTCTGTAGC 
CTTTTAGACA ACCTCTTGGC AGTTACTCCA AGAGATGCTG CAAATGCTTT TCAACAAGCT 
CATCTCATAG AACTCCTCTG TAGCACGGTG GATGCTTCCC TCATAGAAAT GTGCATCCAA 
GAACTCAAGA CCCCACTGCC TTTACCATCT GCTGTTGAAC ATATTCAGGC TCAGGCTTCC 
TTTTTCTTGG AATACCTATC CTCTTTGTCC CAGCTCTTGA AGTCCTGTCT GTTGGTAGAG 
CCTAAACTTG TGACACAAGA TGAGCTCTTG AAACCTCTTA TCACTAATGT CATCCGAGTT 
CTCATTGTGT GTCCCAAAGA TGTATTAGAT GTACAGTTAG TGTTGGCTTT TTACCGCACA 
TGGACGCATT TATTTGACCT TCTGGCCACA CTACTGAGGA AAGCTGGTGC TGTCTCCCTT 
GTTCCTCTTA CCTCAGCTCT TGCCAAGCAT TGGGAGGCAG TGACTGATAC ACTGTGTAGA 
TGTGTGGGCC TCTCATTCAA GTGCCCTGCC CTGACTATTG CCAGCCTCCA GTTCCTGTCT 
GTTCTCTTGG CTGAGGAAGA AAAGAGGCGT GTCCAGGACA AGGACAAAAC AAATGAGGGC 
CAGGCCCCAA CAGTGGCCTT ACTTCTTGAT GGAACTCAGG GAAGCCTTAG CTCTTCAGAG 
CGACTTAATG AAACAATTCT TCAGTGCTAT GAAGGAATAT CCCCCAAAGA TGTCCTGAAA 
AGAGTTGCTG CAAATGCCCT GTTGTCACTT CTGGCTGTTA GCAGAAGAGC GCAGAGGCAT 
GCTTTGAGAT CTGATCTCAT AGAAAACTGT ATGGAGCAGA TGAAGCACAT CAATGCACAA 
CTGAACTTAG ATTTGCTGAG ACCTGGGAAA GCAGTACTGA AAAAAAAGGA GGATGGCTTC 
ATTAAAGAAT TAAGCATCAC CATGCAGCTC CTAAGAAACT GCCTTTATCA AAATGAAGAA 
TGTAAAGTGG CAGCTCTTGA AGCACGTCTT GTGCCTGTAT TGCACTCTCT CTGGCCTTGG 
CTTTTGATGG AAGATTCATT GATGCAAATT GCCTTGCAGT TGCTTTGTGT CTATACTGCA 
AATTTTCCAG CTGGCTGCAG TTCCCTTTGT TGGTCAAATT ATGGACAGTG TATTCAAACT 
GCACACAGAG GCACCCCCAG TAGCTCCCTG ATACTGTGTA TCCTGAAACT GGCTTCTCAG 
ATGCCTGCAG AGAACACCAC CATTCAGCAG TTGGTTTTTA TGTTTCTTTC AAACTTGGCC 
TTATCTCATG ACTGCAAGGG AGTAATTCAA AAGAGCAACT TTCTACAGCA CTTCTTGTCC 
CTGACATTGC CTAAAGGAGG AAACCAACAT CTCAGCACTG TGGCCATTCT GTGGTTGAAG 
TTGCTCCTGA ATATGTCATT TGGAGAAGAT GGACAGCAAA TGATCCTGAG GCTAGATGGC 
TGCTTAGACT TACTCATAGA GATGAGCAAG TACAAGCACA AGAGCAGCCC TCACATGCCT 
CTGCTGATCT TTCATAACAT TTGCTTCAGT CCTGCTAATA AGCCTAAAAT CCTGGCTAAT 
GAAAAAGTTG TTACTCTTCT GGCTGCCTGT TTAGAAAGTG AGAATCAAAC TGCCCAGAGG 
ATTGGAGCAG CTTCACTTTG GGCTCTAACT TACAATTACC AAAAGGCAAA AGCAACTTTG 
AAAAATCCAT CAATAAAAAG AAAAGTAGAT GAAGCATATT CCATAGCAAA GAGAACTCTC 
TCAAACTCAG AAGAAAACCC TCTGAATTCC TACTATTTGA AATGCCTTGA AAATCTAGTA 
CAACTGCTTA ACTATTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nt mutation causing laterality defects associated with deletion of rotatin.
Mammalian genome : official journal of the International Mammalian Genome Society18(5)310-5(2007 May)
Chatterjee B,Richards K,Bucan M,Lo C

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

Rotatin is a novel gene required for axial rotation and left-right specification in mouse embryos.
Mechanisms of development113(1)15-28(2002 Apr)
Faisst AM,Alvarez-Bolado G,Treichel D,Gruss P

Gene trap expression and mutational analysis for genes involved in the development of the mammalian nervous system.
Developmental dynamics : an official publication of the American Association of Anatomists212(2)198-213(1998 Jun)
Stoykova A,Chowdhury K,Bonaldo P,Torres M,Gruss P

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Rttn cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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