Mrgprb2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mrgprb2
Entrez Gene ID	243979
Full Name	MAS-related GPR, member B2
Synonyms	4833406I20Rik,Mgrg14
General protein information	Preferred Names MAS-related GPR, member B2 Names mas-related G-protein coupled receptor member B2 Mas-related G protein-coupled receptor g14
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 B4

mRNA and Protein(s)

mRNA	Protein	Name
NM_175531.4	NP_780740.2	mas-related G-protein coupled receptor member B2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Mrgprb2	NP_780740.2
Pan troglodytes (chimpanzee)	MRGPRX1	XP_001173536.1
	MRGPRX1	NP_001035707.1
Homo sapiens (human)	MRGPRX1	NP_671732.3
Rattus norvegicus (Norway rat)	Mrgprb2	XP_577804.2

Gene Ontology
Bibliography

Related articles in PubMed

Different activation signals induce distinct mast cell degranulation strategies.
Gaudenzio N, Sibilano R, Marichal T, Starkl P, Reber LL, Cenac N, McNeil BD, Dong X, Hernandez JD, Sagi-Eisenberg R, Hammel I, Roers A, Valitutti S, Tsai M, Espinosa E, Galli SJ
The Journal of clinical investigation126(10)3981-3998(2016 Oct)

Identification of a mast-cell-specific receptor crucial for pseudo-allergic drug reactions.
McNeil BD, Pundir P, Meeker S, Han L, Undem BJ, Kulka M, Dong X
Nature519(7542)237-41(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

basic secretagogues activate mouse mast cells in vitro and in vivo through a single receptor, Mrgprb2, the orthologue of the human G-protein-coupled receptor MRGPRX2
Title: Identification of a mast-cell-specific receptor crucial for pseudo-allergic drug reactions.

The following Mrgprb2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mrgprb2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22581	NM_175531.4 Latest version!	Mus musculus MAS-related GPR, member B2 (Mrgprb2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mrgprb2 ( NM_175531.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_780740.2 Mus musculus MAS-related GPR, member B2 (Mrgprb2), mRNA.

Price & Availability↑

CloneID	OMu22581
Clone ID Related Accession (Same CDS sequence)	NM_175531.4
Accession Version	NM_175531.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	mas-related G-protein coupled receptor member B2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK029369.1, BC107391.2 and BX519265.1. On May 1, 2009 this sequence version replaced NM_175531.3. ##Evidence-Data-START## Transcript exon combination :: AK029369.1, BC107391.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN01164133 [ECO:0000348] ##Evidence-Data-END##

ATGAGTGGAG ATTTCCTAAT CAAGAATCTA AGCACCTCAG CCTGGAAAAC GAACATCACA 
GTGCTGAATG GAAGCTACTA CATCGATACT TCAGTTTGTG TCACCAGGAA CCAAGCCATG 
ATTTTGCTTT CCATCATCAT TTCCCTGGTT GGGATGGGAC TAAATGCCAT AGTGCTGTGG 
TTCCTGGGCA TCCGTATGCA CACGAATGCC TTCACTGTCT ACATTCTCAA CCTGGCTATG 
GCTGACTTTC TTTACCTGTG CTCTCAGTTT GTAATTTGTC TTCTTATTGC CTTTTATATC 
TTCTACTCAA TTGACATCAA CATCCCTTTG GTTCTTTATG TTGTGCCAAT ATTTGCTTAT 
CTTTCAGGTC TGAGCATTCT CAGCACCATT AGCATTGAGC GCTGCTTGTC TGTAATATGG 
CCCATTTGGT ATCGCTGTAA ACGTCCAAGA CACACATCAG CTATCACATG TTTTGTGCTT 
TGGGTTATGT CCTTATTGTT GGGTCTCCTG GAAGGGAAGG CATGTGGCTT ACTGTTTAAT 
AGCTTTGACT CTTATTGGTG TGAAACATTT GATGTTATCA CTAATATATG GTCAGTTGTT 
TTTTTTGGTG TTCTCTGTGG GTCTAGCCTC ACCCTGCTTG TCAGGATCTT CTGTGGCTCA 
CAGCGAATTC CTATGACCAG GCTGTATGTG ACTATTACAC TCACAGTCTT GGTCTTCCTG 
ATCTTTGGTC TTCCCTTTGG GATCTATTGG ATACTCTATC AGTGGATTAG CAATTTTTAT 
TATGTTGAAA TTTGTAATTT TTATCTTGAG ATACTATTCC TATCCTGTGT TAACAGCTGT 
ATGAACCCCA TCATTTATTT CCTTGTTGGC TCCATTAGGC ACCGAAGGTT CAGGCGGAAG 
ACTCTCAAGC TACTTCTGCA GAGAGCCATG CAAGACACCC CTGAGGAGGA ACAAAGTGGA 
AATAAGAGTT CTTCAGAACA CCCTGAAGAA CTGGAAACTG TTCAGAGCTG CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_780740.2
CDS	67..1083
Translation	MSGDFLIKNLSTSAWKTNITVLNGSYYIDTSVCVTRNQAMILLSIIISLVGMGLNAIVLWFLGIRMHTNAFTVYILNLAMADFLYLCSQFVICLLIAFYIFYSIDINIPLVLYVVPIFAYLSGLSILSTISIERCLSVIWPIWYRCKRPRHTSAITCFVLWVMSLLLGLLEGKACGLLFNSFDSYWCETFDVITNIWSVVFFGVLCGSSLTLLVRIFCGSQRIPMTRLYVTITLTVLVFLIFGLPFGIYWILYQWISNFYYVEICNFYLEILFLSCVNSCMNPIIYFLVGSIRHRRFRRKTLKLLLQRAMQDTPEEEQSGNKSSSEHPEELETVQSCS

Target ORF information:

RefSeq Version	NM_175531.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus MAS-related GPR, member B2 (Mrgprb2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175531.4

ORF Insert Sequence:

ATGAGTGGAG ATTTCCTAAT CAAGAATCTA AGCACCTCAG CCTGGAAAAC GAACATCACA 
GTGCTGAATG GAAGCTACTA CATCGATACT TCAGTTTGTG TCACCAGGAA CCAAGCCATG 
ATTTTGCTTT CCATCATCAT TTCCCTGGTT GGGATGGGAC TAAATGCCAT AGTGCTGTGG 
TTCCTGGGCA TCCGTATGCA CACGAATGCC TTCACTGTCT ACATTCTCAA CCTGGCTATG 
GCTGACTTTC TTTACCTGTG CTCTCAGTTT GTAATTTGTC TTCTTATTGC CTTTTATATC 
TTCTACTCAA TTGACATCAA CATCCCTTTG GTTCTTTATG TTGTGCCAAT ATTTGCTTAT 
CTTTCAGGTC TGAGCATTCT CAGCACCATT AGCATTGAGC GCTGCTTGTC TGTAATATGG 
CCCATTTGGT ATCGCTGTAA ACGTCCAAGA CACACATCAG CTATCACATG TTTTGTGCTT 
TGGGTTATGT CCTTATTGTT GGGTCTCCTG GAAGGGAAGG CATGTGGCTT ACTGTTTAAT 
AGCTTTGACT CTTATTGGTG TGAAACATTT GATGTTATCA CTAATATATG GTCAGTTGTT 
TTTTTTGGTG TTCTCTGTGG GTCTAGCCTC ACCCTGCTTG TCAGGATCTT CTGTGGCTCA 
CAGCGAATTC CTATGACCAG GCTGTATGTG ACTATTACAC TCACAGTCTT GGTCTTCCTG 
ATCTTTGGTC TTCCCTTTGG GATCTATTGG ATACTCTATC AGTGGATTAG CAATTTTTAT 
TATGTTGAAA TTTGTAATTT TTATCTTGAG ATACTATTCC TATCCTGTGT TAACAGCTGT 
ATGAACCCCA TCATTTATTT CCTTGTTGGC TCCATTAGGC ACCGAAGGTT CAGGCGGAAG 
ACTCTCAAGC TACTTCTGCA GAGAGCCATG CAAGACACCC CTGAGGAGGA ACAAAGTGGA 
AATAAGAGTT CTTCAGAACA CCCTGAAGAA CTGGAAACTG TTCAGAGCTG CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Different activation signals induce distinct mast cell degranulation strategies.
The Journal of clinical investigation126(10)3981-3998(2016 Oct)
Gaudenzio N,Sibilano R,Marichal T,Starkl P,Reber LL,Cenac N,McNeil BD,Dong X,Hernandez JD,Sagi-Eisenberg R,Hammel I,Roers A,Valitutti S,Tsai M,Espinosa E,Galli SJ

Identification of a mast-cell-specific receptor crucial for pseudo-allergic drug reactions.
Nature519(7542)237-41(2015 Mar)
McNeil BD,Pundir P,Meeker S,Han L,Undem BJ,Kulka M,Dong X