Klhl21 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Klhl21
Entrez Gene ID	242785
Full Name	kelch-like 21
Synonyms	1810045K06Rik,D330008A20,mKIAA0469
General protein information	Preferred Names kelch-like 21 Names kelch-like protein 21
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001033352.3	NP_001028524.1	kelch-like protein 21

Pathways from BioSystems

Homologies

Bos taurus (cattle)	KLHL21	NP_001070279.1
Homo sapiens (human)	KLHL21	NP_055666.2
	KLHL21	XP_001094118.1
Canis lupus familiaris (dog)	KLHL21	XP_546754.2
Danio rerio (zebrafish)	klhl21	NP_996964.1
Xenopus tropicalis (tropical clawed frog)	klhl21	XP_004916251.1
Pan troglodytes (chimpanzee)	KLHL21	XP_514342.2
Mus musculus (house mouse)	Klhl21	NP_001028524.1
Rattus norvegicus (Norway rat)	Klhl21	NP_001101466.1
Gallus gallus (chicken)	KLHL21	XP_003642565.1

Gene Ontology
Bibliography

Related articles in PubMed

Squamous cell carcinoma-related oncogene (SCCRO) neddylates Cul3 protein to selectively promote midbody localization and activity of Cul3
Huang G, Kaufman AJ, Xu K, Manova K, Singh B
The Journal of biological chemistry292(37)15254-15265(2017 09)

Kelch-like Protein 21 (KLHL21) Targets I?B Kinase-? to Regulate Nuclear Factor ?-Light Chain Enhancer of Activated B Cells (NF-?B) Signaling Negatively.
Mei ZZ, Chen XY, Hu SW, Wang N, Ou XL, Wang J, Luo HH, Liu J, Jiang Y
The Journal of biological chemistry291(35)18176-89(2016 08)

Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, Kikuno R, Ohara R, Inamoto S, Koseki H, Hiraoka S, Saga Y, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes10(4)167-80(2003 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data (including data from studies using cells cultured from knockout mice) suggest that Sccro neddylates Cul3 with Nedd8, promoting Cul3/Klhl21 complex formation and localization of Cul3 during telophase. (Sccro = squamous cell carcinoma-related oncogene; Cul3 = cullin 3; Nedd8 = neural precursor cell expressed, developmentally down-regulated protein 8; Klhl21 = kelch-like protein 21)
Title: Squamous cell carcinoma-related oncogene (SCCRO) neddylates Cul3 protein to selectively promote midbody localization and activity of Cul3

data clearly demonstrate that KLHL21 negatively regulates TNFalpha-activated NF-kappaB signaling via targeting IKKbeta, providing new insight into the mechanisms underlying NF-kappaB regulation in cells.
Title: Kelch-like Protein 21 (KLHL21) Targets I?B Kinase-? to Regulate Nuclear Factor ?-Light Chain Enhancer of Activated B Cells (NF-?B) Signaling Negatively.

The following Klhl21 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Klhl21 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu12625	NM_001033352.3 Latest version!	Mus musculus kelch-like 21 (Klhl21), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$349.30 $499.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Klhl21 ( NM_001033352.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001028524.1 Mus musculus kelch-like 21 (Klhl21), mRNA.

Price & Availability↑

CloneID	OMu12625
Clone ID Related Accession (Same CDS sequence)	NM_001033352.3
Accession Version	NM_001033352.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	kelch-like protein 21
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL611927.21. On May 2, 2007 this sequence version replaced NM_001033352.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK154493.1, BC060201.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCGGC CGGCGCCTTT GGCTGTGCTG CCCTTCTCCG ATCCCGCGCA CGCCCTAAGC 
CTGTTGCGCG GCCTCAGTCA ACTGCGCGCC GAGCGCAAGT TCCTGGATGT GACCCTGGAG 
GCGGCGGGCG GCCGCGACTT CCCCGCGCAC CGCGCCGTGC TGGCGGCCGC TAGTCCCTAC 
TTCCGCGCCA TGTTCGCGGG CCAGCTGCGC GAGAGCCGCG CCGAGCGCGT GCGCCTGCAC 
GGGGTGCCGC CCGACATGCT GCAGCTGCTC CTGGACTTCA GCTACACCGG CAGAGTGGCG 
GTGAGCGGCG ACAACGCCGA GCCGCTGCTG CGCGCCGCCG ACCTGCTGCA GTTCCCCGCC 
GTGAAGGAGG CGTGCGGCGC CTTCCTGCAG CAGCAGCTCG ACCTGGCCAA CTGCCTGGAC 
ATGCAGGACT TCGCCGAGGC CTTCAGCTGC TCGGGGCTGG CGAGCGCTGC GCAACGCTTC 
ATCCTGCGCC ACGTGGGCGA ACTGGGCGCC GAGCAACTGG AGAGGCTGCC GCTGGCGCGC 
CTGCTGCGCT ACCTGCGCGA CGACGGGCTG TGCGTGCCCA AGGAGGAGGC GGCCTACCAG 
CTGGCGCTGC GCTGGGTGCG CGCGGACCCG CCGCGCCGCG CGCCGCACTG GCCGCAGTTG 
CTGGAGGCTG TGCGCCTGCC CTTCGTGCGC CGCTTCTACC TGCTGGCACA CGTGGAGGCC 
GAGCCACTGG TGGCGCGCTG CCCACCTTGC CTGCGCCTGC TGCGCGAGGC CCGCGACTTC 
CAGGCGGCTC GCTACGACCG GCACGACCGC GGGCCCTGCC CCCGCATGCG TCCGCGCCCG 
TCCACCGGCC TGGCCGAGAT CCTGGTGCTG GTGGGAGGTT GTGACCAGGA CTGCGACGAG 
CTGGTTACGG TCGACTGCTA CAATCCGCAG ACCGGCCAGT GGCGTTACCT GGCCGAGTTC 
CCCGATCACT TAGGTGGGGG CTATAGTATC GTGGCTCTGG GCAACGACAT CTACGTGACG 
GGCGGGTCTG ATGGCTCCCG GCTCTATGAC TGCGTGTGGA GATACAATTC GAGTGTGAAC 
GAGTGGACGG AGGTGGCACC CATGCTCAAG GCCCGAGAGT ACCACAGCTC CTCAGTGCTA 
GACGGCCTTC TGTATGTGGT GGCAGCCGAC AGCACGGAGC GCTATGACCA TGCCACCGAC 
TCCTGGGAGG CACTGCAGCC CATGACCTAC CCCATGGACA ACTGCTCTAC CACTGCCTGC 
CGAGGCCGAC TCTATGCCAT TGGCTCCCTG GCAGGCAAGG AGACCATGGT GATACAGTGC 
TATGACCCAG ACACTGATCT GTGGTCGCTG GTGAACTGTG GCCAGCTCCC ACCGTGGTCC 
TTTGCGCCCA AGACTGTGAC TTTGAATGGA CTCATGTACT TCGTCAGGGA TGACTCTGCC 
GAGGTGGACG TCTACAACCC CACTAAAGAC GAATGGGACA AGATCCCATC CATGAATCAG 
GTACACGTTG GGGGCAGCCT GGCCGTCCTG GGAGGCAAAC TCTATGTCTC TGGGGGGTAC 
GACAACACCT TTGAACTCTC GGACGTGGTG GAGGCATATG ACCCAGAGAC CCGGGCCTGG 
AGTGTGGTGG GGCGGCTTCC AGAGCCCACC TTCTGGCACG GCAGCGTGAG CATCTTCCGC 
CAGTTCATGC CACAGACACC GGCAGGGGGG CGTGGCTTCG AGCTGAACAG TGGTAGCAAT 
GACGTGGATG CAGGCTACCA CAGGCTGCCA CAGAACCCCG AGGAGCTGCA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001028524.1
CDS	53..1846
Translation	MERPAPLAVLPFSDPAHALSLLRGLSQLRAERKFLDVTLEAAGGRDFPAHRAVLAAASPYFRAMFAGQLRESRAERVRLHGVPPDMLQLLLDFSYTGRVAVSGDNAEPLLRAADLLQFPAVKEACGAFLQQQLDLANCLDMQDFAEAFSCSGLASAAQRFILRHVGELGAEQLERLPLARLLRYLRDDGLCVPKEEAAYQLALRWVRADPPRRAPHWPQLLEAVRLPFVRRFYLLAHVEAEPLVARCPPCLRLLREARDFQAARYDRHDRGPCPRMRPRPSTGLAEILVLVGGCDQDCDELVTVDCYNPQTGQWRYLAEFPDHLGGGYSIVALGNDIYVTGGSDGSRLYDCVWRYNSSVNEWTEVAPMLKAREYHSSSVLDGLLYVVAADSTERYDHATDSWEALQPMTYPMDNCSTTACRGRLYAIGSLAGKETMVIQCYDPDTDLWSLVNCGQLPPWSFAPKTVTLNGLMYFVRDDSAEVDVYNPTKDEWDKIPSMNQVHVGGSLAVLGGKLYVSGGYDNTFELSDVVEAYDPETRAWSVVGRLPEPTFWHGSVSIFRQFMPQTPAGGRGFELNSGSNDVDAGYHRLPQNPEELH

Target ORF information:

RefSeq Version	NM_001033352.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kelch-like 21 (Klhl21), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033352.3

ORF Insert Sequence:

ATGGAGCGGC CGGCGCCTTT GGCTGTGCTG CCCTTCTCCG ATCCCGCGCA CGCCCTAAGC 
CTGTTGCGCG GCCTCAGTCA ACTGCGCGCC GAGCGCAAGT TCCTGGATGT GACCCTGGAG 
GCGGCGGGCG GCCGCGACTT CCCCGCGCAC CGCGCCGTGC TGGCGGCCGC TAGTCCCTAC 
TTCCGCGCCA TGTTCGCGGG CCAGCTGCGC GAGAGCCGCG CCGAGCGCGT GCGCCTGCAC 
GGGGTGCCGC CCGACATGCT GCAGCTGCTC CTGGACTTCA GCTACACCGG CAGAGTGGCG 
GTGAGCGGCG ACAACGCCGA GCCGCTGCTG CGCGCCGCCG ACCTGCTGCA GTTCCCCGCC 
GTGAAGGAGG CGTGCGGCGC CTTCCTGCAG CAGCAGCTCG ACCTGGCCAA CTGCCTGGAC 
ATGCAGGACT TCGCCGAGGC CTTCAGCTGC TCGGGGCTGG CGAGCGCTGC GCAACGCTTC 
ATCCTGCGCC ACGTGGGCGA ACTGGGCGCC GAGCAACTGG AGAGGCTGCC GCTGGCGCGC 
CTGCTGCGCT ACCTGCGCGA CGACGGGCTG TGCGTGCCCA AGGAGGAGGC GGCCTACCAG 
CTGGCGCTGC GCTGGGTGCG CGCGGACCCG CCGCGCCGCG CGCCGCACTG GCCGCAGTTG 
CTGGAGGCTG TGCGCCTGCC CTTCGTGCGC CGCTTCTACC TGCTGGCACA CGTGGAGGCC 
GAGCCACTGG TGGCGCGCTG CCCACCTTGC CTGCGCCTGC TGCGCGAGGC CCGCGACTTC 
CAGGCGGCTC GCTACGACCG GCACGACCGC GGGCCCTGCC CCCGCATGCG TCCGCGCCCG 
TCCACCGGCC TGGCCGAGAT CCTGGTGCTG GTGGGAGGTT GTGACCAGGA CTGCGACGAG 
CTGGTTACGG TCGACTGCTA CAATCCGCAG ACCGGCCAGT GGCGTTACCT GGCCGAGTTC 
CCCGATCACT TAGGTGGGGG CTATAGTATC GTGGCTCTGG GCAACGACAT CTACGTGACG 
GGCGGGTCTG ATGGCTCCCG GCTCTATGAC TGCGTGTGGA GATACAATTC GAGTGTGAAC 
GAGTGGACGG AGGTGGCACC CATGCTCAAG GCCCGAGAGT ACCACAGCTC CTCAGTGCTA 
GACGGCCTTC TGTATGTGGT GGCAGCCGAC AGCACGGAGC GCTATGACCA TGCCACCGAC 
TCCTGGGAGG CACTGCAGCC CATGACCTAC CCCATGGACA ACTGCTCTAC CACTGCCTGC 
CGAGGCCGAC TCTATGCCAT TGGCTCCCTG GCAGGCAAGG AGACCATGGT GATACAGTGC 
TATGACCCAG ACACTGATCT GTGGTCGCTG GTGAACTGTG GCCAGCTCCC ACCGTGGTCC 
TTTGCGCCCA AGACTGTGAC TTTGAATGGA CTCATGTACT TCGTCAGGGA TGACTCTGCC 
GAGGTGGACG TCTACAACCC CACTAAAGAC GAATGGGACA AGATCCCATC CATGAATCAG 
GTACACGTTG GGGGCAGCCT GGCCGTCCTG GGAGGCAAAC TCTATGTCTC TGGGGGGTAC 
GACAACACCT TTGAACTCTC GGACGTGGTG GAGGCATATG ACCCAGAGAC CCGGGCCTGG 
AGTGTGGTGG GGCGGCTTCC AGAGCCCACC TTCTGGCACG GCAGCGTGAG CATCTTCCGC 
CAGTTCATGC CACAGACACC GGCAGGGGGG CGTGGCTTCG AGCTGAACAG TGGTAGCAAT 
GACGTGGATG CAGGCTACCA CAGGCTGCCA CAGAACCCCG AGGAGCTGCA TTAG