Sra1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sra1
Entrez Gene ID	24068
Full Name	steroid receptor RNA activator 1
Synonyms	AA959952,Sra,Srap,Straa1,Strra1
General protein information	Preferred Names steroid receptor RNA activator 1 Names steroid receptor RNA activator 1 sra1 steroid receptor RNA activator protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18\|18 B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001164406.1	NP_001157878.1	steroid receptor RNA activator 1 isoform b
NM_025291.3	NP_079567.2	steroid receptor RNA activator 1 isoform a

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SRA1	XP_001137717.2
	SRA1	XP_001086694.2
Homo sapiens (human)	SRA1	NP_001030312.2
Danio rerio (zebrafish)	sra1	NP_001002047.1
Mus musculus (house mouse)	Sra1	NP_079567.2
Canis lupus familiaris (dog)	SRA1	XP_005617340.1
Rattus norvegicus (Norway rat)	Sra1	NP_899158.3
Xenopus tropicalis (tropical clawed frog)	sra1	NP_001107371.1
Bos taurus (cattle)	SRA1	NP_001073110.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

VISFATIN PROMOTES FOAM CELL FORMATION BY DYSREGULATING CD36, SRA, ABCA1, AND ABCG1 EXPRESSION IN RAW264.7 MACROPHAGES.
Lin YT, Jian DY, Kwok CF, Ho LT, Juan CC
Shock (Augusta, Ga.)45(4)460-8(2016 Apr)

Scavenger receptor SRA attenuates microglia activation and protects neuroinflammatory injury in intracerebral hemorrhage.
Yang Z, Zhong S, Liu Y, Shen H, Yuan B
Journal of neuroimmunology278232-8(2015 Jan)

SRA gene knockout protects against diet-induced obesity and improves glucose tolerance.
Liu S, Sheng L, Miao H, Saunders TL, MacDougald OA, Koenig RJ, Xu B
The Journal of biological chemistry289(19)13000-9(2014 May)

RNA helicase Ddx5 and the noncoding RNA SRA act as coactivators in the Notch signaling pathway.
Jung C, Mittler G, Oswald F, Borggrefe T
Biochimica et biophysica acta1833(5)1180-9(2013 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Visfatin upregulated CD36 and SRA expression and downregulated ABCA1 and ABCG1 expression, subsequently increased ox-LDL uptake and decreased cholesterol efflux, and finally promoted foam cell formation via the PI3K- and ERK-dependent pathways.
Title: VISFATIN PROMOTES FOAM CELL FORMATION BY DYSREGULATING CD36, SRA, ABCA1, AND ABCG1 EXPRESSION IN RAW264.7 MACROPHAGES.

The results of this study demonstrated that SRA could attenuate microglia mediated inflammation injury in ICH. In addition, SRA mediated negative feedback mechanism in neuroimmune homeostasis.
Title: Scavenger receptor SRA attenuates microglia activation and protects neuroinflammatory injury in intracerebral hemorrhage.

these data are the first to indicate a functional role for SRA in adipose tissue biology and glucose homeostasis in vivo.
Title: SRA gene knockout protects against diet-induced obesity and improves glucose tolerance.

Dax1 augmentation of LRH-1-mediated Oct4 activation is dependent upon steroid receptor RNA activator
Title: Dax1 up-regulates Oct4 expression in mouse embryonic stem cells via LRH-1 and SRA.

DEAD-box RNA helicase p68 (DDX5) and its associated noncoding RNA, steroid receptor RNA activator (SRA), form a complex with CTCF that is essential for insulator function
Title: Mediation of CTCF transcriptional insulation by DEAD-box RNA-binding protein p68 and steroid receptor RNA activator SRA.

The following Sra1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sra1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu00212	NM_025291.3 Latest version!	Mus musculus steroid receptor RNA activator 1 (Sra1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu09787	NM_001164406.1 Latest version!	Mus musculus steroid receptor RNA activator 1 (Sra1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sra1 ( NM_025291.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079567.2 Mus musculus steroid receptor RNA activator 1 (Sra1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu00212
Clone ID Related Accession (Same CDS sequence)	NM_025291.3
Accession Version	NM_025291.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	steroid receptor RNA activator 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY097874.1, AK010375.1 and BQ748094.1. On Sep 2, 2009 this sequence version replaced NM_025291.2. Transcript Variant: This variant (1) encodes the longer isoform (a). CCDS Note: The coding region has been updated to extend the N-terminus to one that is also supported by available conservation data. The use of an alternative upstream start codon would result in a protein that is 12 aa longer. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK007302.1, DV053615.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGCGCT GCCCCGCTGG CGGTGCGGAA GTGGAGATGG CGGAGCTGTA CGTGAAGCCC 
GGCAACAAGG AACGCGGCTG GAACGACCCG CCACAATTCT CCTACGGGCT CCAGACTCAG 
ACTGGTGGAC CCAAACGCAC TCCCCTTACT AAGAGGGTCG CGGCCCCACA GGATGGATCC 
CCTAGAGCCC CAGAAACTTC TGGACCACCT CCAGTGGATC ATCCACCTCC TTCAAGTAAG 
GCTTCCAGGC CTCCGCCCAT GGGGAGCTGT CCTGCTACTG GTGTGGAGCC CCCAAGTTCC 
CCAGTCATTG AGTCTGAAAC TCTGATAGAA GACGTGCTGA GACCTCTGGA ACAGGCATTG 
GAGGACTGCC ATGGTCACAC AAAGAAACAG GTATGTGATG ATATCAGCCG ACGCTTGGCG 
CTGCTTCGAG AACAGTGGGC TGGAGGGAAG TTGTCAATAC CTGTAAAGAA GAGGATGGCA 
CTGCTAGTGC AAGAACTTTT ACATCACCAG TGGGATGCAG CAGATGACAT TCACCGATCA 
CTCATGGTTG ACCATGTGAC TGAGGTCAGT CAGTGGATGG TGGGAGTAAA AAGATTAATT 
GCAGAAAAGA AGAGTCTATC TTCAGAGGAG ACCAAAGAAG AGAAATTTAC AGTGGAACCT 
GAGAACCAGA CAATACCAGG CTTCCAACAG CCATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079567.2
CDS	4..702
Translation	MMRCPAGGAEVEMAELYVKPGNKERGWNDPPQFSYGLQTQTGGPKRTPLTKRVAAPQDGSPRAPETSGPPPVDHPPPSSKASRPPPMGSCPATGVEPPSSPVIESETLIEDVLRPLEQALEDCHGHTKKQVCDDISRRLALLREQWAGGKLSIPVKKRMALLVQELLHHQWDAADDIHRSLMVDHVTEVSQWMVGVKRLIAEKKSLSSEETKEEKFTVEPENQTIPGFQQPS

Target ORF information:

RefSeq Version	NM_025291.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus steroid receptor RNA activator 1 (Sra1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025291.3

ORF Insert Sequence:

ATGATGCGCT GCCCCGCTGG CGGTGCGGAA GTGGAGATGG CGGAGCTGTA CGTGAAGCCC 
GGCAACAAGG AACGCGGCTG GAACGACCCG CCACAATTCT CCTACGGGCT CCAGACTCAG 
ACTGGTGGAC CCAAACGCAC TCCCCTTACT AAGAGGGTCG CGGCCCCACA GGATGGATCC 
CCTAGAGCCC CAGAAACTTC TGGACCACCT CCAGTGGATC ATCCACCTCC TTCAAGTAAG 
GCTTCCAGGC CTCCGCCCAT GGGGAGCTGT CCTGCTACTG GTGTGGAGCC CCCAAGTTCC 
CCAGTCATTG AGTCTGAAAC TCTGATAGAA GACGTGCTGA GACCTCTGGA ACAGGCATTG 
GAGGACTGCC ATGGTCACAC AAAGAAACAG GTATGTGATG ATATCAGCCG ACGCTTGGCG 
CTGCTTCGAG AACAGTGGGC TGGAGGGAAG TTGTCAATAC CTGTAAAGAA GAGGATGGCA 
CTGCTAGTGC AAGAACTTTT ACATCACCAG TGGGATGCAG CAGATGACAT TCACCGATCA 
CTCATGGTTG ACCATGTGAC TGAGGTCAGT CAGTGGATGG TGGGAGTAAA AAGATTAATT 
GCAGAAAAGA AGAGTCTATC TTCAGAGGAG ACCAAAGAAG AGAAATTTAC AGTGGAACCT 
GAGAACCAGA CAATACCAGG CTTCCAACAG CCATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sra1 ( NM_001164406.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001157878.1 Mus musculus steroid receptor RNA activator 1 (Sra1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu09787
Clone ID Related Accession (Same CDS sequence)	NM_001164406.1
Accession Version	NM_001164406.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	steroid receptor RNA activator 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY097874.1, AK010375.1 and BQ748094.1. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. It encodes isoform b, which has a shorter and distinct C-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CF584304.1, BG087662.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGCGCT GCCCCGCTGG CGGTGCGGAA GTGGAGATGG CGGAGCTGTA CGTGAAGCCC 
GGCAACAAGG AACGCGGCTG GAACGACCCG CCACAATTCT CCTACGGGCT CCAGACTCAG 
ACTGGTGGAC CCAAACGCAC TCCCCTTACT AAGAGGGTCG CGGCCCCACA GGATGGATCC 
CCTAGAGCCC CAGAAACTTC TGGACCACCT CCAGTGGATC ATCCACCTCC TTCAAGTAAG 
GCTTCCAGGC CTCCGCCCAT GGGGAGCTGT CCTGCTACTG GTGTGGAGCC CCCAAGTTCC 
CCAGTCATTG AGTCTGAAAC TCTGATAGAA GACGTGCTGA GACCTCTGGA ACAGGCATTG 
GAGGACTGCC ATGGTCACAC AAAGAAACAG AACTTTTACA TCACCAGTGG GATGCAGCAG 
ATGACATTCA CCGATCACTC ATGGTTGACC ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157878.1
CDS	4..459
Translation	MMRCPAGGAEVEMAELYVKPGNKERGWNDPPQFSYGLQTQTGGPKRTPLTKRVAAPQDGSPRAPETSGPPPVDHPPPSSKASRPPPMGSCPATGVEPPSSPVIESETLIEDVLRPLEQALEDCHGHTKKQNFYITSGMQQMTFTDHSWLTM

Target ORF information:

RefSeq Version	NM_001164406.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus steroid receptor RNA activator 1 (Sra1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164406.1

ORF Insert Sequence:

ATGATGCGCT GCCCCGCTGG CGGTGCGGAA GTGGAGATGG CGGAGCTGTA CGTGAAGCCC 
GGCAACAAGG AACGCGGCTG GAACGACCCG CCACAATTCT CCTACGGGCT CCAGACTCAG 
ACTGGTGGAC CCAAACGCAC TCCCCTTACT AAGAGGGTCG CGGCCCCACA GGATGGATCC 
CCTAGAGCCC CAGAAACTTC TGGACCACCT CCAGTGGATC ATCCACCTCC TTCAAGTAAG 
GCTTCCAGGC CTCCGCCCAT GGGGAGCTGT CCTGCTACTG GTGTGGAGCC CCCAAGTTCC 
CCAGTCATTG AGTCTGAAAC TCTGATAGAA GACGTGCTGA GACCTCTGGA ACAGGCATTG 
GAGGACTGCC ATGGTCACAC AAAGAAACAG AACTTTTACA TCACCAGTGG GATGCAGCAG 
ATGACATTCA CCGATCACTC ATGGTTGACC ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

VISFATIN PROMOTES FOAM CELL FORMATION BY DYSREGULATING CD36, SRA, ABCA1, AND ABCG1 EXPRESSION IN RAW264.7 MACROPHAGES.
Shock (Augusta, Ga.)45(4)460-8(2016 Apr)
Lin YT,Jian DY,Kwok CF,Ho LT,Juan CC

Scavenger receptor SRA attenuates microglia activation and protects neuroinflammatory injury in intracerebral hemorrhage.
Journal of neuroimmunology278232-8(2015 Jan)
Yang Z,Zhong S,Liu Y,Shen H,Yuan B

SRA gene knockout protects against diet-induced obesity and improves glucose tolerance.
The Journal of biological chemistry289(19)13000-9(2014 May)
Liu S,Sheng L,Miao H,Saunders TL,MacDougald OA,Koenig RJ,Xu B

RNA helicase Ddx5 and the noncoding RNA SRA act as coactivators in the Notch signaling pathway.
Biochimica et biophysica acta1833(5)1180-9(2013 May)
Jung C,Mittler G,Oswald F,Borggrefe T