Neu2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Neu2
Entrez Gene ID	23956
Full Name	neuraminidase 2
Synonyms	MBS,MSS,MTS
General protein information	Preferred Names neuraminidase 2 Names sialidase-2 N-acetyl-alpha-neuraminidase 2 brain sialidase cystolic sialidase cytosolic sialidase murine thymic sialidase skeletal muscle sialidase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 D

mRNA and Protein(s)

mRNA	Protein	Name
NM_001160165.1	NP_001153637.1	sialidase-2 isoform 3
XM_006529524.4	XP_006529587.1	sialidase-2 isoform X1
NM_001160163.1	NP_001153635.1	sialidase-2 isoform 1
NM_015750.3	NP_056565.1	sialidase-2 isoform 4
XM_006529524.3	XP_006529587.1	sialidase-2 isoform X1
NM_001160164.1	NP_001153636.1	sialidase-2 isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	NEU2	NP_005374.2
Pan troglodytes (chimpanzee)	NEU2	XP_003309574.1
Canis lupus familiaris (dog)	NEU2	XP_005635919.1
Bos taurus (cattle)	NEU2	XP_003585975.1
Gallus gallus (chicken)	NEU2	XP_004936983.1
Mus musculus (house mouse)	Neu2	NP_001153637.1
Rattus norvegicus (Norway rat)	Neu2	NP_058826.2
	NEU2	XP_001114628.1

Gene Ontology
Bibliography

Related articles in PubMed

Possible association of Neu2 with plasma membrane fraction from mouse thymus exhibited sialidase activity with fetuin at pH 7.0 but not at pH 4.5.
Kijimoto-Ochiai S, Doi N, Fujii M, Go S, Kabayama K, Moriya S, Miyagi T, Koda T
Microbiology and immunology57(8)569-82(2013 Aug)

The Gne M712T mouse as a model for human glomerulopathy.
Kakani S, Yardeni T, Poling J, Ciccone C, Niethamer T, Klootwijk ED, Manoli I, Darvish D, Hoogstraten-Miller S, Zerfas P, Tian E, Ten Hagen KG, Kopp JB, Gahl WA, Huizing M
The American journal of pathology180(4)1431-40(2012 Apr)

Specific expression of Neu2 type B in mouse thymus and the existence of a membrane-bound form in COS cells.
Koda T, Kijimoto-Ochiai S, Uemura S, Inokuchi J
Biochemical and biophysical research communications387(4)729-35(2009 Oct)

Developmental change of sialidase neu4 expression in murine brain and its involvement in the regulation of neuronal cell differentiation.
Shiozaki K, Koseki K, Yamaguchi K, Shiozaki M, Narimatsu H, Miyagi T
The Journal of biological chemistry284(32)21157-64(2009 Aug)

The cytosolic sialidase Neu2 is degraded by autophagy during myoblast atrophy.
Rossi S, Stoppani E, Martinet W, Bonetto A, Costelli P, Giuliani R, Colombo F, Preti A, Marchesini S, Fanzani A
Biochimica et biophysica acta1790(8)817-28(2009 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Neu2 from thymus exhibited sialidase activity with fetuin at pH 7.0.
Title: Possible association of Neu2 with plasma membrane fraction from mouse thymus exhibited sialidase activity with fetuin at pH 7.0 but not at pH 4.5.

30-40% of B and C forms of Neu2 activity was located in the crude membrane-fraction, and hydrolyzed ganglioside effectively, while both soluble fraction showed particular behavior with substrate specificity.
Title: Specific expression of Neu2 type B in mouse thymus and the existence of a membrane-bound form in COS cells.

the lack of soluble sialidase activity in the SM/J mouse thymus is due to the thymus-specific low expression level of the Neu2 gene
Title: Low expression of Neu2 sialidase in the thymus of SM/J mice-existence of neuraminidase positive cells "Neu-medullocyte" in the murine thymus.

Our data demonstrate that IGF-1-induced myoblast differentiation and hypertrophy are driven, at least in part, by Neu2 upregulation and further support the significant role of cytosolic sialidase in myoblasts.
Title: Insulin-like growth factor 1 signaling regulates cytosolic sialidase Neu2 expression during myoblast differentiation and hypertrophy.

The following Neu2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Neu2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15847	NM_001160165.1 Latest version!	Mus musculus neuraminidase 2 (Neu2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu15859	NM_001160164.1 Latest version!	Mus musculus neuraminidase 2 (Neu2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu15859	NM_015750.3 Latest version!	Mus musculus neuraminidase 2 (Neu2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu15985	NM_001160163.1 Latest version!	Mus musculus neuraminidase 2 (Neu2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu15859	XM_006529524.4 Latest version!	Mus musculus neuraminidase 2 (Neu2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu15859	XM_006529524.3	Mus musculus neuraminidase 2 (Neu2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Neu2 ( NM_001160165.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001153637.1 Mus musculus neuraminidase 2 (Neu2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu15847
Clone ID Related Accession (Same CDS sequence)	NM_001160165.1
Accession Version	NM_001160165.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	sialidase-2 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY088515.1, AB048604.1 and AC102564.7. Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region but maintains the reading frame, compared to variant 1. The resulting protein (isoform 3) contains a shorter and distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB048604.1, AF139059.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849383 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGATC TCAGGCCCAT GGCGACCTGC CCTGTCCTGC AGAAGGAGAC ACTGTTCCGC 
ACAGGCGTCC ATGCTTACAG AATCCCTGCT CTGCTCTACC TGAAGAAGCA GAAGACCCTG 
CTGGCCTTTG CGGAAAAGCG AGCCAGCAAG ACGGATGAGC ACGCAGAGTT GATTGTCCTG 
AGAAGAGGAA GCTACAACGA AGCCACCAAC CGTGTCAAGT GGCAGCCTGA GGAAGTGGTG 
ACCCAAGCCC AGCTGGAAGG CCACCGCTCC ATGAATCCAT GTCCCTTGTA TGACAAGCAA 
ACAAAGACCC TCTTCCTTTT CTTCATCGCT GTCCCTGGGC GTGTATCAGA ACATCATCAG 
CTCCACACTA AGGTTAATGT CACACGGCTG TGCTGTGTCA GCAGCACTGA CCATGGGAGG 
ACCTGGAGCC CCATCCAGGA CCTCACAGAG ACCACCATTG GCAGCACTCA TCAGGAATGG 
GCCACATTTG CTGTGGGTCC TGGGCATTGT CTGCAGCTGC GGAACCCAGC TGGGAGCCTG 
CTGGTACCTG CTTATGCCTA CCGGAAACTG CACCCTGCTC AGAAGCCTAC CCCCTTTGCC 
TTCTGCTTCA TCAGCCTTGA CCATGGGCAC ACATGGAAAC TAGGCAACTT TGTGGCTGAA 
AACTCACTGG AGTGCCAGGT GGCTGAGGTT GGCACTGGAG CTCAGAGGAT GGTATATCTC 
AATGCTAGGA GCTTCCTGGG AGCCAGGGTC CAGGCACAAA GTCCTAATGA TGGTCTGGAT 
TTCCAGGACA ACCGGGTAGT GAGTAAGCTT GTAGAGCCCC CCCACGGGTG TCATGGAAGT 
GTGGTTGCCT TCCACAACCC CATCTCTAAG CCACATGCCT TAGACACATG GCTTCTTTAT 
ACACACCCTA CAGACTCCAG GAATAGAACC AACCTGGGTG TGTACCTAAA CCAGATGCCA 
CTAGATCCCA CAGCCTGGTC AGAGCCCACC CTGCTGGCCA TGGGCATCTG TGCCTACTCA 
GACTTACAGA ACATGGGGCA AGGCCCTGAT GGCTCCCCAC AGTTTGGGTG TCTGTATGAA 
TCAGGTAACT ATGAAGAGAT CATTTTCCTC ATATTCACCC TGAAGCAAGC TTTCCCCACT 
GTATTTGATG CCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153637.1
CDS	222..1379
Translation	MEDLRPMATCPVLQKETLFRTGVHAYRIPALLYLKKQKTLLAFAEKRASKTDEHAELIVLRRGSYNEATNRVKWQPEEVVTQAQLEGHRSMNPCPLYDKQTKTLFLFFIAVPGRVSEHHQLHTKVNVTRLCCVSSTDHGRTWSPIQDLTETTIGSTHQEWATFAVGPGHCLQLRNPAGSLLVPAYAYRKLHPAQKPTPFAFCFISLDHGHTWKLGNFVAENSLECQVAEVGTGAQRMVYLNARSFLGARVQAQSPNDGLDFQDNRVVSKLVEPPHGCHGSVVAFHNPISKPHALDTWLLYTHPTDSRNRTNLGVYLNQMPLDPTAWSEPTLLAMGICAYSDLQNMGQGPDGSPQFGCLYESGNYEEIIFLIFTLKQAFPTVFDAQ

Target ORF information:

RefSeq Version	NM_001160165.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neuraminidase 2 (Neu2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160165.1

ORF Insert Sequence:

ATGGAAGATC TCAGGCCCAT GGCGACCTGC CCTGTCCTGC AGAAGGAGAC ACTGTTCCGC 
ACAGGCGTCC ATGCTTACAG AATCCCTGCT CTGCTCTACC TGAAGAAGCA GAAGACCCTG 
CTGGCCTTTG CGGAAAAGCG AGCCAGCAAG ACGGATGAGC ACGCAGAGTT GATTGTCCTG 
AGAAGAGGAA GCTACAACGA AGCCACCAAC CGTGTCAAGT GGCAGCCTGA GGAAGTGGTG 
ACCCAAGCCC AGCTGGAAGG CCACCGCTCC ATGAATCCAT GTCCCTTGTA TGACAAGCAA 
ACAAAGACCC TCTTCCTTTT CTTCATCGCT GTCCCTGGGC GTGTATCAGA ACATCATCAG 
CTCCACACTA AGGTTAATGT CACACGGCTG TGCTGTGTCA GCAGCACTGA CCATGGGAGG 
ACCTGGAGCC CCATCCAGGA CCTCACAGAG ACCACCATTG GCAGCACTCA TCAGGAATGG 
GCCACATTTG CTGTGGGTCC TGGGCATTGT CTGCAGCTGC GGAACCCAGC TGGGAGCCTG 
CTGGTACCTG CTTATGCCTA CCGGAAACTG CACCCTGCTC AGAAGCCTAC CCCCTTTGCC 
TTCTGCTTCA TCAGCCTTGA CCATGGGCAC ACATGGAAAC TAGGCAACTT TGTGGCTGAA 
AACTCACTGG AGTGCCAGGT GGCTGAGGTT GGCACTGGAG CTCAGAGGAT GGTATATCTC 
AATGCTAGGA GCTTCCTGGG AGCCAGGGTC CAGGCACAAA GTCCTAATGA TGGTCTGGAT 
TTCCAGGACA ACCGGGTAGT GAGTAAGCTT GTAGAGCCCC CCCACGGGTG TCATGGAAGT 
GTGGTTGCCT TCCACAACCC CATCTCTAAG CCACATGCCT TAGACACATG GCTTCTTTAT 
ACACACCCTA CAGACTCCAG GAATAGAACC AACCTGGGTG TGTACCTAAA CCAGATGCCA 
CTAGATCCCA CAGCCTGGTC AGAGCCCACC CTGCTGGCCA TGGGCATCTG TGCCTACTCA 
GACTTACAGA ACATGGGGCA AGGCCCTGAT GGCTCCCCAC AGTTTGGGTG TCTGTATGAA 
TCAGGTAACT ATGAAGAGAT CATTTTCCTC ATATTCACCC TGAAGCAAGC TTTCCCCACT 
GTATTTGATG CCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Neu2 ( NM_015750.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_056565.1 Mus musculus neuraminidase 2 (Neu2), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu15859
Clone ID Related Accession (Same CDS sequence)	XM_006529524.4 , NM_015750.3 , XM_006529524.3 , NM_001160164.1
Accession Version	NM_015750.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	sialidase-2 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB028023.1 and AC102564.7. On May 14, 2009 this sequence version replaced NM_015750.2. Transcript Variant: This variant (4) differs in the 5' UTR and 5' coding region but maintains the reading frame, compared to variant 1. The resulting protein (isoform 4) contains a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB028023.1, BC105657.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849376 [ECO:0000348] ##Evidence-Data-END##

ATGGCGACCT GCCCTGTCCT GCAGAAGGAG ACACTGTTCC GCACAGGCGT CCATGCTTAC 
AGAATCCCTG CTCTGCTCTA CCTGAAGAAG CAGAAGACCC TGCTGGCCTT TGCGGAAAAG 
CGAGCCAGCA AGACGGATGA GCACGCAGAG TTGATTGTCC TGAGAAGAGG AAGCTACAAC 
GAAGCCACCA ACCGTGTCAA GTGGCAGCCT GAGGAAGTGG TGACCCAAGC CCAGCTGGAA 
GGCCACCGCT CCATGAATCC ATGTCCCTTG TATGACAAGC AAACAAAGAC CCTCTTCCTT 
TTCTTCATCG CTGTCCCTGG GCGTGTATCA GAACATCATC AGCTCCACAC TAAGGTTAAT 
GTCACACGGC TGTGCTGTGT CAGCAGCACT GACCATGGGA GGACCTGGAG CCCCATCCAG 
GACCTCACAG AGACCACCAT TGGCAGCACT CATCAGGAAT GGGCCACATT TGCTGTGGGT 
CCTGGGCATT GTCTGCAGCT GCGGAACCCA GCTGGGAGCC TGCTGGTACC TGCTTATGCC 
TACCGGAAAC TGCACCCTGC TCAGAAGCCT ACCCCCTTTG CCTTCTGCTT CATCAGCCTT 
GACCATGGGC ACACATGGAA ACTAGGCAAC TTTGTGGCTG AAAACTCACT GGAGTGCCAG 
GTGGCTGAGG TTGGCACTGG AGCTCAGAGG ATGGTATATC TCAATGCTAG GAGCTTCCTG 
GGAGCCAGGG TCCAGGCACA AAGTCCTAAT GATGGTCTGG ATTTCCAGGA CAACCGGGTA 
GTGAGTAAGC TTGTAGAGCC CCCCCACGGG TGTCATGGAA GTGTGGTTGC CTTCCACAAC 
CCCATCTCTA AGCCACATGC CTTAGACACA TGGCTTCTTT ATACACACCC TACAGACTCC 
AGGAATAGAA CCAACCTGGG TGTGTACCTA AACCAGATGC CACTAGATCC CACAGCCTGG 
TCAGAGCCCA CCCTGCTGGC CATGGGCATC TGTGCCTACT CAGACTTACA GAACATGGGG 
CAAGGCCCTG ATGGCTCCCC ACAGTTTGGG TGTCTGTATG AATCAGGTAA CTATGAAGAG 
ATCATTTTCC TCATATTCAC CCTGAAGCAA GCTTTCCCCA CTGTATTTGA TGCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056565.1
CDS	80..1219
Translation	MATCPVLQKETLFRTGVHAYRIPALLYLKKQKTLLAFAEKRASKTDEHAELIVLRRGSYNEATNRVKWQPEEVVTQAQLEGHRSMNPCPLYDKQTKTLFLFFIAVPGRVSEHHQLHTKVNVTRLCCVSSTDHGRTWSPIQDLTETTIGSTHQEWATFAVGPGHCLQLRNPAGSLLVPAYAYRKLHPAQKPTPFAFCFISLDHGHTWKLGNFVAENSLECQVAEVGTGAQRMVYLNARSFLGARVQAQSPNDGLDFQDNRVVSKLVEPPHGCHGSVVAFHNPISKPHALDTWLLYTHPTDSRNRTNLGVYLNQMPLDPTAWSEPTLLAMGICAYSDLQNMGQGPDGSPQFGCLYESGNYEEIIFLIFTLKQAFPTVFDAQ

Target ORF information:

RefSeq Version	NM_015750.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus neuraminidase 2 (Neu2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015750.3

ORF Insert Sequence:

ATGGCGACCT GCCCTGTCCT GCAGAAGGAG ACACTGTTCC GCACAGGCGT CCATGCTTAC 
AGAATCCCTG CTCTGCTCTA CCTGAAGAAG CAGAAGACCC TGCTGGCCTT TGCGGAAAAG 
CGAGCCAGCA AGACGGATGA GCACGCAGAG TTGATTGTCC TGAGAAGAGG AAGCTACAAC 
GAAGCCACCA ACCGTGTCAA GTGGCAGCCT GAGGAAGTGG TGACCCAAGC CCAGCTGGAA 
GGCCACCGCT CCATGAATCC ATGTCCCTTG TATGACAAGC AAACAAAGAC CCTCTTCCTT 
TTCTTCATCG CTGTCCCTGG GCGTGTATCA GAACATCATC AGCTCCACAC TAAGGTTAAT 
GTCACACGGC TGTGCTGTGT CAGCAGCACT GACCATGGGA GGACCTGGAG CCCCATCCAG 
GACCTCACAG AGACCACCAT TGGCAGCACT CATCAGGAAT GGGCCACATT TGCTGTGGGT 
CCTGGGCATT GTCTGCAGCT GCGGAACCCA GCTGGGAGCC TGCTGGTACC TGCTTATGCC 
TACCGGAAAC TGCACCCTGC TCAGAAGCCT ACCCCCTTTG CCTTCTGCTT CATCAGCCTT 
GACCATGGGC ACACATGGAA ACTAGGCAAC TTTGTGGCTG AAAACTCACT GGAGTGCCAG 
GTGGCTGAGG TTGGCACTGG AGCTCAGAGG ATGGTATATC TCAATGCTAG GAGCTTCCTG 
GGAGCCAGGG TCCAGGCACA AAGTCCTAAT GATGGTCTGG ATTTCCAGGA CAACCGGGTA 
GTGAGTAAGC TTGTAGAGCC CCCCCACGGG TGTCATGGAA GTGTGGTTGC CTTCCACAAC 
CCCATCTCTA AGCCACATGC CTTAGACACA TGGCTTCTTT ATACACACCC TACAGACTCC 
AGGAATAGAA CCAACCTGGG TGTGTACCTA AACCAGATGC CACTAGATCC CACAGCCTGG 
TCAGAGCCCA CCCTGCTGGC CATGGGCATC TGTGCCTACT CAGACTTACA GAACATGGGG 
CAAGGCCCTG ATGGCTCCCC ACAGTTTGGG TGTCTGTATG AATCAGGTAA CTATGAAGAG 
ATCATTTTCC TCATATTCAC CCTGAAGCAA GCTTTCCCCA CTGTATTTGA TGCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Neu2 ( NM_001160164.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001153636.1 Mus musculus neuraminidase 2 (Neu2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu15859
Clone ID Related Accession (Same CDS sequence)	XM_006529524.4 , NM_015750.3 , XM_006529524.3 , NM_001160164.1
Accession Version	NM_001160164.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Mus musculus(house mouse)
Product	sialidase-2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK145284.1, AB048604.1 and AC102564.7. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region and uses a downstream start codon compared to variant 1. Isoform 2 has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK145284.1, BY026109.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849376 [ECO:0000348] ##Evidence-Data-END##

ATGGCGACCT GCCCTGTCCT GCAGAAGGAG ACACTGTTCC GCACAGGCGT CCATGCTTAC 
AGAATCCCTG CTCTGCTCTA CCTGAAGAAG CAGAAGACCC TGCTGGCCTT TGCGGAAAAG 
CGAGCCAGCA AGACGGATGA GCACGCAGAG TTGATTGTCC TGAGAAGAGG AAGCTACAAC 
GAAGCCACCA ACCGTGTCAA GTGGCAGCCT GAGGAAGTGG TGACCCAAGC CCAGCTGGAA 
GGCCACCGCT CCATGAATCC ATGTCCCTTG TATGACAAGC AAACAAAGAC CCTCTTCCTT 
TTCTTCATCG CTGTCCCTGG GCGTGTATCA GAACATCATC AGCTCCACAC TAAGGTTAAT 
GTCACACGGC TGTGCTGTGT CAGCAGCACT GACCATGGGA GGACCTGGAG CCCCATCCAG 
GACCTCACAG AGACCACCAT TGGCAGCACT CATCAGGAAT GGGCCACATT TGCTGTGGGT 
CCTGGGCATT GTCTGCAGCT GCGGAACCCA GCTGGGAGCC TGCTGGTACC TGCTTATGCC 
TACCGGAAAC TGCACCCTGC TCAGAAGCCT ACCCCCTTTG CCTTCTGCTT CATCAGCCTT 
GACCATGGGC ACACATGGAA ACTAGGCAAC TTTGTGGCTG AAAACTCACT GGAGTGCCAG 
GTGGCTGAGG TTGGCACTGG AGCTCAGAGG ATGGTATATC TCAATGCTAG GAGCTTCCTG 
GGAGCCAGGG TCCAGGCACA AAGTCCTAAT GATGGTCTGG ATTTCCAGGA CAACCGGGTA 
GTGAGTAAGC TTGTAGAGCC CCCCCACGGG TGTCATGGAA GTGTGGTTGC CTTCCACAAC 
CCCATCTCTA AGCCACATGC CTTAGACACA TGGCTTCTTT ATACACACCC TACAGACTCC 
AGGAATAGAA CCAACCTGGG TGTGTACCTA AACCAGATGC CACTAGATCC CACAGCCTGG 
TCAGAGCCCA CCCTGCTGGC CATGGGCATC TGTGCCTACT CAGACTTACA GAACATGGGG 
CAAGGCCCTG ATGGCTCCCC ACAGTTTGGG TGTCTGTATG AATCAGGTAA CTATGAAGAG 
ATCATTTTCC TCATATTCAC CCTGAAGCAA GCTTTCCCCA CTGTATTTGA TGCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153636.1
CDS	112..1251
Translation	MATCPVLQKETLFRTGVHAYRIPALLYLKKQKTLLAFAEKRASKTDEHAELIVLRRGSYNEATNRVKWQPEEVVTQAQLEGHRSMNPCPLYDKQTKTLFLFFIAVPGRVSEHHQLHTKVNVTRLCCVSSTDHGRTWSPIQDLTETTIGSTHQEWATFAVGPGHCLQLRNPAGSLLVPAYAYRKLHPAQKPTPFAFCFISLDHGHTWKLGNFVAENSLECQVAEVGTGAQRMVYLNARSFLGARVQAQSPNDGLDFQDNRVVSKLVEPPHGCHGSVVAFHNPISKPHALDTWLLYTHPTDSRNRTNLGVYLNQMPLDPTAWSEPTLLAMGICAYSDLQNMGQGPDGSPQFGCLYESGNYEEIIFLIFTLKQAFPTVFDAQ

Target ORF information:

RefSeq Version	NM_001160164.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neuraminidase 2 (Neu2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160164.1

ORF Insert Sequence:

ATGGCGACCT GCCCTGTCCT GCAGAAGGAG ACACTGTTCC GCACAGGCGT CCATGCTTAC 
AGAATCCCTG CTCTGCTCTA CCTGAAGAAG CAGAAGACCC TGCTGGCCTT TGCGGAAAAG 
CGAGCCAGCA AGACGGATGA GCACGCAGAG TTGATTGTCC TGAGAAGAGG AAGCTACAAC 
GAAGCCACCA ACCGTGTCAA GTGGCAGCCT GAGGAAGTGG TGACCCAAGC CCAGCTGGAA 
GGCCACCGCT CCATGAATCC ATGTCCCTTG TATGACAAGC AAACAAAGAC CCTCTTCCTT 
TTCTTCATCG CTGTCCCTGG GCGTGTATCA GAACATCATC AGCTCCACAC TAAGGTTAAT 
GTCACACGGC TGTGCTGTGT CAGCAGCACT GACCATGGGA GGACCTGGAG CCCCATCCAG 
GACCTCACAG AGACCACCAT TGGCAGCACT CATCAGGAAT GGGCCACATT TGCTGTGGGT 
CCTGGGCATT GTCTGCAGCT GCGGAACCCA GCTGGGAGCC TGCTGGTACC TGCTTATGCC 
TACCGGAAAC TGCACCCTGC TCAGAAGCCT ACCCCCTTTG CCTTCTGCTT CATCAGCCTT 
GACCATGGGC ACACATGGAA ACTAGGCAAC TTTGTGGCTG AAAACTCACT GGAGTGCCAG 
GTGGCTGAGG TTGGCACTGG AGCTCAGAGG ATGGTATATC TCAATGCTAG GAGCTTCCTG 
GGAGCCAGGG TCCAGGCACA AAGTCCTAAT GATGGTCTGG ATTTCCAGGA CAACCGGGTA 
GTGAGTAAGC TTGTAGAGCC CCCCCACGGG TGTCATGGAA GTGTGGTTGC CTTCCACAAC 
CCCATCTCTA AGCCACATGC CTTAGACACA TGGCTTCTTT ATACACACCC TACAGACTCC 
AGGAATAGAA CCAACCTGGG TGTGTACCTA AACCAGATGC CACTAGATCC CACAGCCTGG 
TCAGAGCCCA CCCTGCTGGC CATGGGCATC TGTGCCTACT CAGACTTACA GAACATGGGG 
CAAGGCCCTG ATGGCTCCCC ACAGTTTGGG TGTCTGTATG AATCAGGTAA CTATGAAGAG 
ATCATTTTCC TCATATTCAC CCTGAAGCAA GCTTTCCCCA CTGTATTTGA TGCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Neu2 ( NM_001160163.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001153635.1 Mus musculus neuraminidase 2 (Neu2), transcript variant 1, mRNA.

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CloneID	OMu15985
Clone ID Related Accession (Same CDS sequence)	NM_001160163.1
Accession Version	NM_001160163.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	sialidase-2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK145284.1, FJ613504.1, AC102564.7 and AB028023.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FJ613504.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849376 [ECO:0000348] ##Evidence-Data-END##

ATGACAGTCC AACCTTCTCC ATGGTTTTCC GATCTCAGGC CCATGGCGAC CTGCCCTGTC 
CTGCAGAAGG AGACACTGTT CCGCACAGGC GTCCATGCTT ACAGAATCCC TGCTCTGCTC 
TACCTGAAGA AGCAGAAGAC CCTGCTGGCC TTTGCGGAAA AGCGAGCCAG CAAGACGGAT 
GAGCACGCAG AGTTGATTGT CCTGAGAAGA GGAAGCTACA ACGAAGCCAC CAACCGTGTC 
AAGTGGCAGC CTGAGGAAGT GGTGACCCAA GCCCAGCTGG AAGGCCACCG CTCCATGAAT 
CCATGTCCCT TGTATGACAA GCAAACAAAG ACCCTCTTCC TTTTCTTCAT CGCTGTCCCT 
GGGCGTGTAT CAGAACATCA TCAGCTCCAC ACTAAGGTTA ATGTCACACG GCTGTGCTGT 
GTCAGCAGCA CTGACCATGG GAGGACCTGG AGCCCCATCC AGGACCTCAC AGAGACCACC 
ATTGGCAGCA CTCATCAGGA ATGGGCCACA TTTGCTGTGG GTCCTGGGCA TTGTCTGCAG 
CTGCGGAACC CAGCTGGGAG CCTGCTGGTA CCTGCTTATG CCTACCGGAA ACTGCACCCT 
GCTCAGAAGC CTACCCCCTT TGCCTTCTGC TTCATCAGCC TTGACCATGG GCACACATGG 
AAACTAGGCA ACTTTGTGGC TGAAAACTCA CTGGAGTGCC AGGTGGCTGA GGTTGGCACT 
GGAGCTCAGA GGATGGTATA TCTCAATGCT AGGAGCTTCC TGGGAGCCAG GGTCCAGGCA 
CAAAGTCCTA ATGATGGTCT GGATTTCCAG GACAACCGGG TAGTGAGTAA GCTTGTAGAG 
CCCCCCCACG GGTGTCATGG AAGTGTGGTT GCCTTCCACA ACCCCATCTC TAAGCCACAT 
GCCTTAGACA CATGGCTTCT TTATACACAC CCTACAGACT CCAGGAATAG AACCAACCTG 
GGTGTGTACC TAAACCAGAT GCCACTAGAT CCCACAGCCT GGTCAGAGCC CACCCTGCTG 
GCCATGGGCA TCTGTGCCTA CTCAGACTTA CAGAACATGG GGCAAGGCCC TGATGGCTCC 
CCACAGTTTG GGTGTCTGTA TGAATCAGGT AACTATGAAG AGATCATTTT CCTCATATTC 
ACCCTGAAGC AAGCTTTCCC CACTGTATTT GATGCCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153635.1
CDS	172..1353
Translation	MTVQPSPWFSDLRPMATCPVLQKETLFRTGVHAYRIPALLYLKKQKTLLAFAEKRASKTDEHAELIVLRRGSYNEATNRVKWQPEEVVTQAQLEGHRSMNPCPLYDKQTKTLFLFFIAVPGRVSEHHQLHTKVNVTRLCCVSSTDHGRTWSPIQDLTETTIGSTHQEWATFAVGPGHCLQLRNPAGSLLVPAYAYRKLHPAQKPTPFAFCFISLDHGHTWKLGNFVAENSLECQVAEVGTGAQRMVYLNARSFLGARVQAQSPNDGLDFQDNRVVSKLVEPPHGCHGSVVAFHNPISKPHALDTWLLYTHPTDSRNRTNLGVYLNQMPLDPTAWSEPTLLAMGICAYSDLQNMGQGPDGSPQFGCLYESGNYEEIIFLIFTLKQAFPTVFDAQ

Target ORF information:

RefSeq Version	NM_001160163.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neuraminidase 2 (Neu2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160163.1

ORF Insert Sequence:

ATGACAGTCC AACCTTCTCC ATGGTTTTCC GATCTCAGGC CCATGGCGAC CTGCCCTGTC 
CTGCAGAAGG AGACACTGTT CCGCACAGGC GTCCATGCTT ACAGAATCCC TGCTCTGCTC 
TACCTGAAGA AGCAGAAGAC CCTGCTGGCC TTTGCGGAAA AGCGAGCCAG CAAGACGGAT 
GAGCACGCAG AGTTGATTGT CCTGAGAAGA GGAAGCTACA ACGAAGCCAC CAACCGTGTC 
AAGTGGCAGC CTGAGGAAGT GGTGACCCAA GCCCAGCTGG AAGGCCACCG CTCCATGAAT 
CCATGTCCCT TGTATGACAA GCAAACAAAG ACCCTCTTCC TTTTCTTCAT CGCTGTCCCT 
GGGCGTGTAT CAGAACATCA TCAGCTCCAC ACTAAGGTTA ATGTCACACG GCTGTGCTGT 
GTCAGCAGCA CTGACCATGG GAGGACCTGG AGCCCCATCC AGGACCTCAC AGAGACCACC 
ATTGGCAGCA CTCATCAGGA ATGGGCCACA TTTGCTGTGG GTCCTGGGCA TTGTCTGCAG 
CTGCGGAACC CAGCTGGGAG CCTGCTGGTA CCTGCTTATG CCTACCGGAA ACTGCACCCT 
GCTCAGAAGC CTACCCCCTT TGCCTTCTGC TTCATCAGCC TTGACCATGG GCACACATGG 
AAACTAGGCA ACTTTGTGGC TGAAAACTCA CTGGAGTGCC AGGTGGCTGA GGTTGGCACT 
GGAGCTCAGA GGATGGTATA TCTCAATGCT AGGAGCTTCC TGGGAGCCAG GGTCCAGGCA 
CAAAGTCCTA ATGATGGTCT GGATTTCCAG GACAACCGGG TAGTGAGTAA GCTTGTAGAG 
CCCCCCCACG GGTGTCATGG AAGTGTGGTT GCCTTCCACA ACCCCATCTC TAAGCCACAT 
GCCTTAGACA CATGGCTTCT TTATACACAC CCTACAGACT CCAGGAATAG AACCAACCTG 
GGTGTGTACC TAAACCAGAT GCCACTAGAT CCCACAGCCT GGTCAGAGCC CACCCTGCTG 
GCCATGGGCA TCTGTGCCTA CTCAGACTTA CAGAACATGG GGCAAGGCCC TGATGGCTCC 
CCACAGTTTG GGTGTCTGTA TGAATCAGGT AACTATGAAG AGATCATTTT CCTCATATTC 
ACCCTGAAGC AAGCTTTCCC CACTGTATTT GATGCCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Neu2 ( XM_006529524.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529587.1 Mus musculus neuraminidase 2 (Neu2), transcript variant X1, mRNA.

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CloneID	OMu15859
Clone ID Related Accession (Same CDS sequence)	XM_006529524.4 , NM_015750.3 , XM_006529524.3 , NM_001160164.1
Accession Version	XM_006529524.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	sialidase-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529524.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCT GCCCTGTCCT GCAGAAGGAG ACACTGTTCC GCACAGGCGT CCATGCTTAC 
AGAATCCCTG CTCTGCTCTA CCTGAAGAAG CAGAAGACCC TGCTGGCCTT TGCGGAAAAG 
CGAGCCAGCA AGACGGATGA GCACGCAGAG TTGATTGTCC TGAGAAGAGG AAGCTACAAC 
GAAGCCACCA ACCGTGTCAA GTGGCAGCCT GAGGAAGTGG TGACCCAAGC CCAGCTGGAA 
GGCCACCGCT CCATGAATCC ATGTCCCTTG TATGACAAGC AAACAAAGAC CCTCTTCCTT 
TTCTTCATCG CTGTCCCTGG GCGTGTATCA GAACATCATC AGCTCCACAC TAAGGTTAAT 
GTCACACGGC TGTGCTGTGT CAGCAGCACT GACCATGGGA GGACCTGGAG CCCCATCCAG 
GACCTCACAG AGACCACCAT TGGCAGCACT CATCAGGAAT GGGCCACATT TGCTGTGGGT 
CCTGGGCATT GTCTGCAGCT GCGGAACCCA GCTGGGAGCC TGCTGGTACC TGCTTATGCC 
TACCGGAAAC TGCACCCTGC TCAGAAGCCT ACCCCCTTTG CCTTCTGCTT CATCAGCCTT 
GACCATGGGC ACACATGGAA ACTAGGCAAC TTTGTGGCTG AAAACTCACT GGAGTGCCAG 
GTGGCTGAGG TTGGCACTGG AGCTCAGAGG ATGGTATATC TCAATGCTAG GAGCTTCCTG 
GGAGCCAGGG TCCAGGCACA AAGTCCTAAT GATGGTCTGG ATTTCCAGGA CAACCGGGTA 
GTGAGTAAGC TTGTAGAGCC CCCCCACGGG TGTCATGGAA GTGTGGTTGC CTTCCACAAC 
CCCATCTCTA AGCCACATGC CTTAGACACA TGGCTTCTTT ATACACACCC TACAGACTCC 
AGGAATAGAA CCAACCTGGG TGTGTACCTA AACCAGATGC CACTAGATCC CACAGCCTGG 
TCAGAGCCCA CCCTGCTGGC CATGGGCATC TGTGCCTACT CAGACTTACA GAACATGGGG 
CAAGGCCCTG ATGGCTCCCC ACAGTTTGGG TGTCTGTATG AATCAGGTAA CTATGAAGAG 
ATCATTTTCC TCATATTCAC CCTGAAGCAA GCTTTCCCCA CTGTATTTGA TGCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529587.1
CDS	102..1241
Translation	MATCPVLQKETLFRTGVHAYRIPALLYLKKQKTLLAFAEKRASKTDEHAELIVLRRGSYNEATNRVKWQPEEVVTQAQLEGHRSMNPCPLYDKQTKTLFLFFIAVPGRVSEHHQLHTKVNVTRLCCVSSTDHGRTWSPIQDLTETTIGSTHQEWATFAVGPGHCLQLRNPAGSLLVPAYAYRKLHPAQKPTPFAFCFISLDHGHTWKLGNFVAENSLECQVAEVGTGAQRMVYLNARSFLGARVQAQSPNDGLDFQDNRVVSKLVEPPHGCHGSVVAFHNPISKPHALDTWLLYTHPTDSRNRTNLGVYLNQMPLDPTAWSEPTLLAMGICAYSDLQNMGQGPDGSPQFGCLYESGNYEEIIFLIFTLKQAFPTVFDAQ

Target ORF information:

RefSeq Version	XM_006529524.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus neuraminidase 2 (Neu2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529524.3

ORF Insert Sequence:

ATGGCGACCT GCCCTGTCCT GCAGAAGGAG ACACTGTTCC GCACAGGCGT CCATGCTTAC 
AGAATCCCTG CTCTGCTCTA CCTGAAGAAG CAGAAGACCC TGCTGGCCTT TGCGGAAAAG 
CGAGCCAGCA AGACGGATGA GCACGCAGAG TTGATTGTCC TGAGAAGAGG AAGCTACAAC 
GAAGCCACCA ACCGTGTCAA GTGGCAGCCT GAGGAAGTGG TGACCCAAGC CCAGCTGGAA 
GGCCACCGCT CCATGAATCC ATGTCCCTTG TATGACAAGC AAACAAAGAC CCTCTTCCTT 
TTCTTCATCG CTGTCCCTGG GCGTGTATCA GAACATCATC AGCTCCACAC TAAGGTTAAT 
GTCACACGGC TGTGCTGTGT CAGCAGCACT GACCATGGGA GGACCTGGAG CCCCATCCAG 
GACCTCACAG AGACCACCAT TGGCAGCACT CATCAGGAAT GGGCCACATT TGCTGTGGGT 
CCTGGGCATT GTCTGCAGCT GCGGAACCCA GCTGGGAGCC TGCTGGTACC TGCTTATGCC 
TACCGGAAAC TGCACCCTGC TCAGAAGCCT ACCCCCTTTG CCTTCTGCTT CATCAGCCTT 
GACCATGGGC ACACATGGAA ACTAGGCAAC TTTGTGGCTG AAAACTCACT GGAGTGCCAG 
GTGGCTGAGG TTGGCACTGG AGCTCAGAGG ATGGTATATC TCAATGCTAG GAGCTTCCTG 
GGAGCCAGGG TCCAGGCACA AAGTCCTAAT GATGGTCTGG ATTTCCAGGA CAACCGGGTA 
GTGAGTAAGC TTGTAGAGCC CCCCCACGGG TGTCATGGAA GTGTGGTTGC CTTCCACAAC 
CCCATCTCTA AGCCACATGC CTTAGACACA TGGCTTCTTT ATACACACCC TACAGACTCC 
AGGAATAGAA CCAACCTGGG TGTGTACCTA AACCAGATGC CACTAGATCC CACAGCCTGG 
TCAGAGCCCA CCCTGCTGGC CATGGGCATC TGTGCCTACT CAGACTTACA GAACATGGGG 
CAAGGCCCTG ATGGCTCCCC ACAGTTTGGG TGTCTGTATG AATCAGGTAA CTATGAAGAG 
ATCATTTTCC TCATATTCAC CCTGAAGCAA GCTTTCCCCA CTGTATTTGA TGCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Neu2 ( XM_006529524.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529587.1 Mus musculus neuraminidase 2 (Neu2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu15859
Clone ID Related Accession (Same CDS sequence)	XM_006529524.4 , NM_015750.3 , XM_006529524.3 , NM_001160164.1
Accession Version	XM_006529524.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	sialidase-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529524.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCT GCCCTGTCCT GCAGAAGGAG ACACTGTTCC GCACAGGCGT CCATGCTTAC 
AGAATCCCTG CTCTGCTCTA CCTGAAGAAG CAGAAGACCC TGCTGGCCTT TGCGGAAAAG 
CGAGCCAGCA AGACGGATGA GCACGCAGAG TTGATTGTCC TGAGAAGAGG AAGCTACAAC 
GAAGCCACCA ACCGTGTCAA GTGGCAGCCT GAGGAAGTGG TGACCCAAGC CCAGCTGGAA 
GGCCACCGCT CCATGAATCC ATGTCCCTTG TATGACAAGC AAACAAAGAC CCTCTTCCTT 
TTCTTCATCG CTGTCCCTGG GCGTGTATCA GAACATCATC AGCTCCACAC TAAGGTTAAT 
GTCACACGGC TGTGCTGTGT CAGCAGCACT GACCATGGGA GGACCTGGAG CCCCATCCAG 
GACCTCACAG AGACCACCAT TGGCAGCACT CATCAGGAAT GGGCCACATT TGCTGTGGGT 
CCTGGGCATT GTCTGCAGCT GCGGAACCCA GCTGGGAGCC TGCTGGTACC TGCTTATGCC 
TACCGGAAAC TGCACCCTGC TCAGAAGCCT ACCCCCTTTG CCTTCTGCTT CATCAGCCTT 
GACCATGGGC ACACATGGAA ACTAGGCAAC TTTGTGGCTG AAAACTCACT GGAGTGCCAG 
GTGGCTGAGG TTGGCACTGG AGCTCAGAGG ATGGTATATC TCAATGCTAG GAGCTTCCTG 
GGAGCCAGGG TCCAGGCACA AAGTCCTAAT GATGGTCTGG ATTTCCAGGA CAACCGGGTA 
GTGAGTAAGC TTGTAGAGCC CCCCCACGGG TGTCATGGAA GTGTGGTTGC CTTCCACAAC 
CCCATCTCTA AGCCACATGC CTTAGACACA TGGCTTCTTT ATACACACCC TACAGACTCC 
AGGAATAGAA CCAACCTGGG TGTGTACCTA AACCAGATGC CACTAGATCC CACAGCCTGG 
TCAGAGCCCA CCCTGCTGGC CATGGGCATC TGTGCCTACT CAGACTTACA GAACATGGGG 
CAAGGCCCTG ATGGCTCCCC ACAGTTTGGG TGTCTGTATG AATCAGGTAA CTATGAAGAG 
ATCATTTTCC TCATATTCAC CCTGAAGCAA GCTTTCCCCA CTGTATTTGA TGCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529587.1
CDS	97..1236
Translation	MATCPVLQKETLFRTGVHAYRIPALLYLKKQKTLLAFAEKRASKTDEHAELIVLRRGSYNEATNRVKWQPEEVVTQAQLEGHRSMNPCPLYDKQTKTLFLFFIAVPGRVSEHHQLHTKVNVTRLCCVSSTDHGRTWSPIQDLTETTIGSTHQEWATFAVGPGHCLQLRNPAGSLLVPAYAYRKLHPAQKPTPFAFCFISLDHGHTWKLGNFVAENSLECQVAEVGTGAQRMVYLNARSFLGARVQAQSPNDGLDFQDNRVVSKLVEPPHGCHGSVVAFHNPISKPHALDTWLLYTHPTDSRNRTNLGVYLNQMPLDPTAWSEPTLLAMGICAYSDLQNMGQGPDGSPQFGCLYESGNYEEIIFLIFTLKQAFPTVFDAQ

Target ORF information:

RefSeq Version	XM_006529524.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus neuraminidase 2 (Neu2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529524.4

ORF Insert Sequence:

ATGGCGACCT GCCCTGTCCT GCAGAAGGAG ACACTGTTCC GCACAGGCGT CCATGCTTAC 
AGAATCCCTG CTCTGCTCTA CCTGAAGAAG CAGAAGACCC TGCTGGCCTT TGCGGAAAAG 
CGAGCCAGCA AGACGGATGA GCACGCAGAG TTGATTGTCC TGAGAAGAGG AAGCTACAAC 
GAAGCCACCA ACCGTGTCAA GTGGCAGCCT GAGGAAGTGG TGACCCAAGC CCAGCTGGAA 
GGCCACCGCT CCATGAATCC ATGTCCCTTG TATGACAAGC AAACAAAGAC CCTCTTCCTT 
TTCTTCATCG CTGTCCCTGG GCGTGTATCA GAACATCATC AGCTCCACAC TAAGGTTAAT 
GTCACACGGC TGTGCTGTGT CAGCAGCACT GACCATGGGA GGACCTGGAG CCCCATCCAG 
GACCTCACAG AGACCACCAT TGGCAGCACT CATCAGGAAT GGGCCACATT TGCTGTGGGT 
CCTGGGCATT GTCTGCAGCT GCGGAACCCA GCTGGGAGCC TGCTGGTACC TGCTTATGCC 
TACCGGAAAC TGCACCCTGC TCAGAAGCCT ACCCCCTTTG CCTTCTGCTT CATCAGCCTT 
GACCATGGGC ACACATGGAA ACTAGGCAAC TTTGTGGCTG AAAACTCACT GGAGTGCCAG 
GTGGCTGAGG TTGGCACTGG AGCTCAGAGG ATGGTATATC TCAATGCTAG GAGCTTCCTG 
GGAGCCAGGG TCCAGGCACA AAGTCCTAAT GATGGTCTGG ATTTCCAGGA CAACCGGGTA 
GTGAGTAAGC TTGTAGAGCC CCCCCACGGG TGTCATGGAA GTGTGGTTGC CTTCCACAAC 
CCCATCTCTA AGCCACATGC CTTAGACACA TGGCTTCTTT ATACACACCC TACAGACTCC 
AGGAATAGAA CCAACCTGGG TGTGTACCTA AACCAGATGC CACTAGATCC CACAGCCTGG 
TCAGAGCCCA CCCTGCTGGC CATGGGCATC TGTGCCTACT CAGACTTACA GAACATGGGG 
CAAGGCCCTG ATGGCTCCCC ACAGTTTGGG TGTCTGTATG AATCAGGTAA CTATGAAGAG 
ATCATTTTCC TCATATTCAC CCTGAAGCAA GCTTTCCCCA CTGTATTTGA TGCCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Possible association of Neu2 with plasma membrane fraction from mouse thymus exhibited sialidase activity with fetuin at pH 7.0 but not at pH 4.5.
Microbiology and immunology57(8)569-82(2013 Aug)
Kijimoto-Ochiai S,Doi N,Fujii M,Go S,Kabayama K,Moriya S,Miyagi T,Koda T

The Gne M712T mouse as a model for human glomerulopathy.
The American journal of pathology180(4)1431-40(2012 Apr)
Kakani S,Yardeni T,Poling J,Ciccone C,Niethamer T,Klootwijk ED,Manoli I,Darvish D,Hoogstraten-Miller S,Zerfas P,Tian E,Ten Hagen KG,Kopp JB,Gahl WA,Huizing M

Specific expression of Neu2 type B in mouse thymus and the existence of a membrane-bound form in COS cells.
Biochemical and biophysical research communications387(4)729-35(2009 Oct)
Koda T,Kijimoto-Ochiai S,Uemura S,Inokuchi J

Developmental change of sialidase neu4 expression in murine brain and its involvement in the regulation of neuronal cell differentiation.
The Journal of biological chemistry284(32)21157-64(2009 Aug)
Shiozaki K,Koseki K,Yamaguchi K,Shiozaki M,Narimatsu H,Miyagi T

The cytosolic sialidase Neu2 is degraded by autophagy during myoblast atrophy.
Biochimica et biophysica acta1790(8)817-28(2009 Aug)
Rossi S,Stoppani E,Martinet W,Bonetto A,Costelli P,Giuliani R,Colombo F,Preti A,Marchesini S,Fanzani A

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Neu2 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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