Prss50 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prss50
Entrez Gene ID	235631
Full Name	protease, serine 50
Synonyms	79H19C,Tsp50
General protein information	Preferred Names protease, serine 50 Names probable threonine protease PRSS50 serine protease 50 testes-specific protease 50 testis-specific protease-like protein 50
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9 F2\|9 60.79 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006512099.2	XP_006512162.1	probable threonine protease PRSS50 isoform X1
XM_006512099.3	XP_006512162.1	probable threonine protease PRSS50 isoform X1
NM_146227.4	NP_666339.2	probable threonine protease PRSS50 precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PRSS50	XP_002697138.2
Mus musculus (house mouse)	Prss50	NP_666339.2
Pan troglodytes (chimpanzee)	PRSS50	XP_003309820.1
Rattus norvegicus (Norway rat)	LOC100910205	XP_003750640.2
Homo sapiens (human)	PRSS50	NP_037402.1
Canis lupus familiaris (dog)	PRSS50	XP_005632682.1

Gene Ontology
Bibliography

Related articles in PubMed

The recently identified modifier of murine metastable epialleles, Rearranged L-Myc Fusion, is involved in maintaining epigenetic marks at CpG island shores and enhancers.
Harten SK, Oey H, Bourke LM, Bharti V, Isbel L, Daxinger L, Faou P, Robertson N, Matthews JM, Whitelaw E
BMC biology1321(2015 Mar)

Transcription factor BORIS (Brother of the Regulator of Imprinted Sites) directly induces expression of a cancer-testis antigen, TSP50, through regulated binding of BORIS to the promoter.
Kosaka-Suzuki N, Suzuki T, Pugacheva EM, Vostrov AA, Morse HC 3rd, Loukinov D, Lobanenkov V
The Journal of biological chemistry286(31)27378-88(2011 Aug)

Testes-specific protease 50 (TSP50) promotes cell proliferation through the activation of the nuclear factor ?B (NF-?B) signalling pathway.
Song ZB, Bao YL, Zhang Y, Mi XG, Wu P, Wu Y, Yu CL, Sun Y, Zheng LH, Huang YX, Liu B, Li YX
The Biochemical journal436(2)457-67(2011 Jun)

Knockdown of TSP50 inhibits cell proliferation and induces apoptosis in P19 cells.
Zhou L, Bao YL, Zhang Y, Wu Y, Yu CL, Huang YX, Sun Y, Zheng LH, Li YX
IUBMB life62(11)825-32(2010 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings indicate that BORIS-induced expression of TSP50 is governed by accessibility and binding of BORIS to the promoter
Title: Transcription factor BORIS (Brother of the Regulator of Imprinted Sites) directly induces expression of a cancer-testis antigen, TSP50, through regulated binding of BORIS to the promoter.

TSP50 promotes cell proliferation, at least partially, through activation of the NF-kappaB signalling pathway.
Title: Testes-specific protease 50 (TSP50) promotes cell proliferation through the activation of the nuclear factor ?B (NF-?B) signalling pathway.

results showed that downregulation of TSP50 expression in P19 embryonal carcinoma stem cells not only reduced cell proliferation, colony formation, and migration but also induced cell apoptosis
Title: Knockdown of TSP50 inhibits cell proliferation and induces apoptosis in P19 cells.

These findings suggested that DNA methylation might regulate the TSP50 and mTSP50 gene expressions in different types of tissues and spermatic cells.
Title: Differential methylation of TSP50 and mTSP50 genes in different types of human tissues and mouse spermatic cells.

TSP50 may perform a critical biologic function in mammalian spermatogenesis. It may be a new type of threonine protease whose linear catalytic structure is similar to many serine proteases, except for a Thr-for-Ser residual catalytic site substitution.
Title: Localization and expression of TSP50 protein in human and rodent testes.

The following Prss50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prss50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu43403	XM_006512099.3 Latest version!	Mus musculus protease, serine 50 (Prss50), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu12332	NM_146227.4 Latest version!	Mus musculus protease, serine 50 (Prss50), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
View Old Accession Versions >>
OMu43403	XM_006512099.2	Mus musculus protease, serine 50 (Prss50), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prss50 ( XM_006512099.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512162.1 Mus musculus protease, serine 50 (Prss50), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu43403
Clone ID Related Accession (Same CDS sequence)	XM_006512099.2 , XM_006512099.3
Accession Version	XM_006512099.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	probable threonine protease PRSS50 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006512099.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCGCC CGATGGAGGG CGCCATTGTG GAGGAACCTA GCGGCGCAAT GGAGCCCTGG 
TGCGGGGCAG AGGTCCGTGG GCAGGGCCCT CAGGGTCCCC GTGTGCCTGG GGCTTCCCGC 
TCCCGCTCCC GCGCCCTTCT CCTGCTGTTG CTGCTGCTGC TGCTGCTCCT GCCTCGGCGG 
CCGGCAGGCT ACTTGGCCGC AGGAGCATCC CCGGGGACGC TGTCCACCAC CGTCCCGACC 
GGACCCGGGG TCTCCTGTGG CTCCAGAGGC ATCTGTCCCT CAGGCAGGCT TCGCCTTCCT 
CGGCAAGCCC AGACTAACCA GACCACGACG GCTCCACCGA ACTCACAGAC CATGGCTCCA 
CTGAAAACGG TGGGGACTCT CGGCATGATG GACACTACTG GGTCCGTTCT TAAGACGGTT 
CATTCCAGCA ACCTCCCCTT CTGTGGCTCC TCCCACGAGC CAGACCCTAC TCTCAGGGAC 
CCAGAAGCCA TGACTCGGCG GTGGCCCTGG ATGGTCAGCG TGCAGGCTAA TGGCTCACAC 
ATCTGTGCTG GCATCCTTAT CGCTTCCCAG TGGGTGCTGA CCGTGGCCCA TTGCTTGAGC 
CAGAACCATG TTAACTACAT AGTGAGGGCG GGGAGCCCGT GGATTAATCA GACGGCAGGA 
ACCAGCTCAG ATGTGCCGGT CCATCGAGTC ATCATAAACC ATGGCTACCA ACCCAGACGG 
TACTGGTCAT GGGTTGGCCG GGCCCATGAC ATCGGCCTTC TCAAGCTCAA GTGGGGGCTC 
AAGTACAGTA AATACGTGTG GCCCATCTGC CTGCCTGGCC TGGATTACGT GGTGGAGGAC 
AGTTCTCTCT GCACTGTGAC AGGCTGGGGA TATCCCAGGG CTAACGGCAT CTGGCCCCAG 
TTCCAGTCCC TCCAGGAGAA GGAAGTCTCT ATCCTGAACA GCAAGAAATG TGATCATTTC 
TACCACAAGT TCTCCAGAAT CTCCTCTCTG GTTCGGATCA TCAACCCTCA GATGATCTGT 
GCCTCGGACA ACAACAGGGA GGAGTTCTGC TATGAGATAA CTGGCGAGCC CCTGGTCTGC 
TCTTCAGATG GCACATGGTA CCTGGTGGGA ATGATGAGCT GGGGCCCAGG CTGCAAGAAG 
AGCGAGGCCC CACCCATCTT TCTGCAGGTC TCCTACTACA GGCCCTGGAT CTGGGACCGG 
CTCAGTGGGG AGCCCCTGGC CCTTCCAGCC CCATCCAGGA CCTTGCTCCT GGCTTTCCTT 
CTGCTCCTCA TCCTTCTGGG CACACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512162.1
CDS	45..1334
Translation	MRRPMEGAIVEEPSGAMEPWCGAEVRGQGPQGPRVPGASRSRSRALLLLLLLLLLLLPRRPAGYLAAGASPGTLSTTVPTGPGVSCGSRGICPSGRLRLPRQAQTNQTTTAPPNSQTMAPLKTVGTLGMMDTTGSVLKTVHSSNLPFCGSSHEPDPTLRDPEAMTRRWPWMVSVQANGSHICAGILIASQWVLTVAHCLSQNHVNYIVRAGSPWINQTAGTSSDVPVHRVIINHGYQPRRYWSWVGRAHDIGLLKLKWGLKYSKYVWPICLPGLDYVVEDSSLCTVTGWGYPRANGIWPQFQSLQEKEVSILNSKKCDHFYHKFSRISSLVRIINPQMICASDNNREEFCYEITGEPLVCSSDGTWYLVGMMSWGPGCKKSEAPPIFLQVSYYRPWIWDRLSGEPLALPAPSRTLLLAFLLLLILLGTL

Target ORF information:

RefSeq Version	XM_006512099.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protease, serine 50 (Prss50), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512099.2

ORF Insert Sequence:

ATGAGGCGCC CGATGGAGGG CGCCATTGTG GAGGAACCTA GCGGCGCAAT GGAGCCCTGG 
TGCGGGGCAG AGGTCCGTGG GCAGGGCCCT CAGGGTCCCC GTGTGCCTGG GGCTTCCCGC 
TCCCGCTCCC GCGCCCTTCT CCTGCTGTTG CTGCTGCTGC TGCTGCTCCT GCCTCGGCGG 
CCGGCAGGCT ACTTGGCCGC AGGAGCATCC CCGGGGACGC TGTCCACCAC CGTCCCGACC 
GGACCCGGGG TCTCCTGTGG CTCCAGAGGC ATCTGTCCCT CAGGCAGGCT TCGCCTTCCT 
CGGCAAGCCC AGACTAACCA GACCACGACG GCTCCACCGA ACTCACAGAC CATGGCTCCA 
CTGAAAACGG TGGGGACTCT CGGCATGATG GACACTACTG GGTCCGTTCT TAAGACGGTT 
CATTCCAGCA ACCTCCCCTT CTGTGGCTCC TCCCACGAGC CAGACCCTAC TCTCAGGGAC 
CCAGAAGCCA TGACTCGGCG GTGGCCCTGG ATGGTCAGCG TGCAGGCTAA TGGCTCACAC 
ATCTGTGCTG GCATCCTTAT CGCTTCCCAG TGGGTGCTGA CCGTGGCCCA TTGCTTGAGC 
CAGAACCATG TTAACTACAT AGTGAGGGCG GGGAGCCCGT GGATTAATCA GACGGCAGGA 
ACCAGCTCAG ATGTGCCGGT CCATCGAGTC ATCATAAACC ATGGCTACCA ACCCAGACGG 
TACTGGTCAT GGGTTGGCCG GGCCCATGAC ATCGGCCTTC TCAAGCTCAA GTGGGGGCTC 
AAGTACAGTA AATACGTGTG GCCCATCTGC CTGCCTGGCC TGGATTACGT GGTGGAGGAC 
AGTTCTCTCT GCACTGTGAC AGGCTGGGGA TATCCCAGGG CTAACGGCAT CTGGCCCCAG 
TTCCAGTCCC TCCAGGAGAA GGAAGTCTCT ATCCTGAACA GCAAGAAATG TGATCATTTC 
TACCACAAGT TCTCCAGAAT CTCCTCTCTG GTTCGGATCA TCAACCCTCA GATGATCTGT 
GCCTCGGACA ACAACAGGGA GGAGTTCTGC TATGAGATAA CTGGCGAGCC CCTGGTCTGC 
TCTTCAGATG GCACATGGTA CCTGGTGGGA ATGATGAGCT GGGGCCCAGG CTGCAAGAAG 
AGCGAGGCCC CACCCATCTT TCTGCAGGTC TCCTACTACA GGCCCTGGAT CTGGGACCGG 
CTCAGTGGGG AGCCCCTGGC CCTTCCAGCC CCATCCAGGA CCTTGCTCCT GGCTTTCCTT 
CTGCTCCTCA TCCTTCTGGG CACACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prss50 ( XM_006512099.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512162.1 Mus musculus protease, serine 50 (Prss50), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu43403
Clone ID Related Accession (Same CDS sequence)	XM_006512099.2 , XM_006512099.3
Accession Version	XM_006512099.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	probable threonine protease PRSS50 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512099.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCGCC CGATGGAGGG CGCCATTGTG GAGGAACCTA GCGGCGCAAT GGAGCCCTGG 
TGCGGGGCAG AGGTCCGTGG GCAGGGCCCT CAGGGTCCCC GTGTGCCTGG GGCTTCCCGC 
TCCCGCTCCC GCGCCCTTCT CCTGCTGTTG CTGCTGCTGC TGCTGCTCCT GCCTCGGCGG 
CCGGCAGGCT ACTTGGCCGC AGGAGCATCC CCGGGGACGC TGTCCACCAC CGTCCCGACC 
GGACCCGGGG TCTCCTGTGG CTCCAGAGGC ATCTGTCCCT CAGGCAGGCT TCGCCTTCCT 
CGGCAAGCCC AGACTAACCA GACCACGACG GCTCCACCGA ACTCACAGAC CATGGCTCCA 
CTGAAAACGG TGGGGACTCT CGGCATGATG GACACTACTG GGTCCGTTCT TAAGACGGTT 
CATTCCAGCA ACCTCCCCTT CTGTGGCTCC TCCCACGAGC CAGACCCTAC TCTCAGGGAC 
CCAGAAGCCA TGACTCGGCG GTGGCCCTGG ATGGTCAGCG TGCAGGCTAA TGGCTCACAC 
ATCTGTGCTG GCATCCTTAT CGCTTCCCAG TGGGTGCTGA CCGTGGCCCA TTGCTTGAGC 
CAGAACCATG TTAACTACAT AGTGAGGGCG GGGAGCCCGT GGATTAATCA GACGGCAGGA 
ACCAGCTCAG ATGTGCCGGT CCATCGAGTC ATCATAAACC ATGGCTACCA ACCCAGACGG 
TACTGGTCAT GGGTTGGCCG GGCCCATGAC ATCGGCCTTC TCAAGCTCAA GTGGGGGCTC 
AAGTACAGTA AATACGTGTG GCCCATCTGC CTGCCTGGCC TGGATTACGT GGTGGAGGAC 
AGTTCTCTCT GCACTGTGAC AGGCTGGGGA TATCCCAGGG CTAACGGCAT CTGGCCCCAG 
TTCCAGTCCC TCCAGGAGAA GGAAGTCTCT ATCCTGAACA GCAAGAAATG TGATCATTTC 
TACCACAAGT TCTCCAGAAT CTCCTCTCTG GTTCGGATCA TCAACCCTCA GATGATCTGT 
GCCTCGGACA ACAACAGGGA GGAGTTCTGC TATGAGATAA CTGGCGAGCC CCTGGTCTGC 
TCTTCAGATG GCACATGGTA CCTGGTGGGA ATGATGAGCT GGGGCCCAGG CTGCAAGAAG 
AGCGAGGCCC CACCCATCTT TCTGCAGGTC TCCTACTACA GGCCCTGGAT CTGGGACCGG 
CTCAGTGGGG AGCCCCTGGC CCTTCCAGCC CCATCCAGGA CCTTGCTCCT GGCTTTCCTT 
CTGCTCCTCA TCCTTCTGGG CACACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512162.1
CDS	42..1331
Translation	MRRPMEGAIVEEPSGAMEPWCGAEVRGQGPQGPRVPGASRSRSRALLLLLLLLLLLLPRRPAGYLAAGASPGTLSTTVPTGPGVSCGSRGICPSGRLRLPRQAQTNQTTTAPPNSQTMAPLKTVGTLGMMDTTGSVLKTVHSSNLPFCGSSHEPDPTLRDPEAMTRRWPWMVSVQANGSHICAGILIASQWVLTVAHCLSQNHVNYIVRAGSPWINQTAGTSSDVPVHRVIINHGYQPRRYWSWVGRAHDIGLLKLKWGLKYSKYVWPICLPGLDYVVEDSSLCTVTGWGYPRANGIWPQFQSLQEKEVSILNSKKCDHFYHKFSRISSLVRIINPQMICASDNNREEFCYEITGEPLVCSSDGTWYLVGMMSWGPGCKKSEAPPIFLQVSYYRPWIWDRLSGEPLALPAPSRTLLLAFLLLLILLGTL

Target ORF information:

RefSeq Version	XM_006512099.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus protease, serine 50 (Prss50), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512099.3

ORF Insert Sequence:

ATGAGGCGCC CGATGGAGGG CGCCATTGTG GAGGAACCTA GCGGCGCAAT GGAGCCCTGG 
TGCGGGGCAG AGGTCCGTGG GCAGGGCCCT CAGGGTCCCC GTGTGCCTGG GGCTTCCCGC 
TCCCGCTCCC GCGCCCTTCT CCTGCTGTTG CTGCTGCTGC TGCTGCTCCT GCCTCGGCGG 
CCGGCAGGCT ACTTGGCCGC AGGAGCATCC CCGGGGACGC TGTCCACCAC CGTCCCGACC 
GGACCCGGGG TCTCCTGTGG CTCCAGAGGC ATCTGTCCCT CAGGCAGGCT TCGCCTTCCT 
CGGCAAGCCC AGACTAACCA GACCACGACG GCTCCACCGA ACTCACAGAC CATGGCTCCA 
CTGAAAACGG TGGGGACTCT CGGCATGATG GACACTACTG GGTCCGTTCT TAAGACGGTT 
CATTCCAGCA ACCTCCCCTT CTGTGGCTCC TCCCACGAGC CAGACCCTAC TCTCAGGGAC 
CCAGAAGCCA TGACTCGGCG GTGGCCCTGG ATGGTCAGCG TGCAGGCTAA TGGCTCACAC 
ATCTGTGCTG GCATCCTTAT CGCTTCCCAG TGGGTGCTGA CCGTGGCCCA TTGCTTGAGC 
CAGAACCATG TTAACTACAT AGTGAGGGCG GGGAGCCCGT GGATTAATCA GACGGCAGGA 
ACCAGCTCAG ATGTGCCGGT CCATCGAGTC ATCATAAACC ATGGCTACCA ACCCAGACGG 
TACTGGTCAT GGGTTGGCCG GGCCCATGAC ATCGGCCTTC TCAAGCTCAA GTGGGGGCTC 
AAGTACAGTA AATACGTGTG GCCCATCTGC CTGCCTGGCC TGGATTACGT GGTGGAGGAC 
AGTTCTCTCT GCACTGTGAC AGGCTGGGGA TATCCCAGGG CTAACGGCAT CTGGCCCCAG 
TTCCAGTCCC TCCAGGAGAA GGAAGTCTCT ATCCTGAACA GCAAGAAATG TGATCATTTC 
TACCACAAGT TCTCCAGAAT CTCCTCTCTG GTTCGGATCA TCAACCCTCA GATGATCTGT 
GCCTCGGACA ACAACAGGGA GGAGTTCTGC TATGAGATAA CTGGCGAGCC CCTGGTCTGC 
TCTTCAGATG GCACATGGTA CCTGGTGGGA ATGATGAGCT GGGGCCCAGG CTGCAAGAAG 
AGCGAGGCCC CACCCATCTT TCTGCAGGTC TCCTACTACA GGCCCTGGAT CTGGGACCGG 
CTCAGTGGGG AGCCCCTGGC CCTTCCAGCC CCATCCAGGA CCTTGCTCCT GGCTTTCCTT 
CTGCTCCTCA TCCTTCTGGG CACACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prss50 ( NM_146227.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666339.2 Mus musculus protease, serine 50 (Prss50), mRNA.

Price & Availability↑

CloneID	OMu12332
Clone ID Related Accession (Same CDS sequence)	NM_146227.4
Accession Version	NM_146227.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	probable threonine protease PRSS50 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC139378.4. On May 1, 2007 this sequence version replaced NM_146227.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK045168.1, BC150686.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN00849388 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCCCT GGTGCGGGGC AGAGGTCCGT GGGCAGGGCC CTCAGGGTCC CCGTGTGCCT 
GGGGCTTCCC GCTCCCGCTC CCGCGCCCTT CTCCTGCTGT TGCTGCTGCT GCTGCTGCTC 
CTGCCTCGGC GGCCGGCAGG TGAGCGCATC CGCCCCCGAC GCCCTCCCCG GCACGCCCAC 
CCGCGGCCAC CTCTGACTCG CTGGAGACCT TCCACAGGCT ACTTGGCCGC AGGAGCATCC 
CCGGGGACGC TGTCCACCAC CGTCCCGACC GGACCCGGGG TCTCCTGTGG CTCCAGAGGC 
ATCTGTCCCT CAGGCAGGCT TCGCCTTCCT CGGCAAGCCC AGACTAACCA GACCACGACG 
GCTCCACCGA ACTCACAGAC CATGGCTCCA CTGAAAACGG TGGGGACTCT CGGCATGATG 
GACACTACTG GGTCCGTTCT TAAGACGGTT CATTCCAGCA ACCTCCCCTT CTGTGGCTCC 
TCCCACGAGC CAGACCCTAC TCTCAGGGAC CCAGAAGCCA TGACTCGGCG GTGGCCCTGG 
ATGGTCAGCG TGCAGGCTAA TGGCTCACAC ATCTGTGCTG GCATCCTTAT CGCTTCCCAG 
TGGGTGCTGA CCGTGGCCCA TTGCTTGAGC CAGAACCATG TTAACTACAT AGTGAGGGCG 
GGGAGCCCGT GGATTAATCA GACGGCAGGA ACCAGCTCAG ATGTGCCGGT CCATCGAGTC 
ATCATAAACC ATGGCTACCA ACCCAGACGG TACTGGTCAT GGGTTGGCCG GGCCCATGAC 
ATCGGCCTTC TCAAGCTCAA GTGGGGGCTC AAGTACAGTA AATACGTGTG GCCCATCTGC 
CTGCCTGGCC TGGATTACGT GGTGGAGGAC AGTTCTCTCT GCACTGTGAC AGGCTGGGGA 
TATCCCAGGG CTAACGGCAT CTGGCCCCAG TTCCAGTCCC TCCAGGAGAA GGAAGTCTCT 
ATCCTGAACA GCAAGAAATG TGATCATTTC TACCACAAGT TCTCCAGAAT CTCCTCTCTG 
GTTCGGATCA TCAACCCTCA GATGATCTGT GCCTCGGACA ACAACAGGGA GGAGTTCTGC 
TATGAGATAA CTGGCGAGCC CCTGGTCTGC TCTTCAGATG GCACATGGTA CCTGGTGGGA 
ATGATGAGCT GGGGCCCAGG CTGCAAGAAG AGCGAGGCCC CACCCATCTT TCTGCAGGTC 
TCCTACTACA GGCCCTGGAT CTGGGACCGG CTCAGTGGGG AGCCCCTGGC CCTTCCAGCC 
CCATCCAGGA CCTTGCTCCT GGCTTTCCTT CTGCTCCTCA TCCTTCTGGG CACACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666339.2
CDS	25..1344
Translation	MEPWCGAEVRGQGPQGPRVPGASRSRSRALLLLLLLLLLLLPRRPAGERIRPRRPPRHAHPRPPLTRWRPSTGYLAAGASPGTLSTTVPTGPGVSCGSRGICPSGRLRLPRQAQTNQTTTAPPNSQTMAPLKTVGTLGMMDTTGSVLKTVHSSNLPFCGSSHEPDPTLRDPEAMTRRWPWMVSVQANGSHICAGILIASQWVLTVAHCLSQNHVNYIVRAGSPWINQTAGTSSDVPVHRVIINHGYQPRRYWSWVGRAHDIGLLKLKWGLKYSKYVWPICLPGLDYVVEDSSLCTVTGWGYPRANGIWPQFQSLQEKEVSILNSKKCDHFYHKFSRISSLVRIINPQMICASDNNREEFCYEITGEPLVCSSDGTWYLVGMMSWGPGCKKSEAPPIFLQVSYYRPWIWDRLSGEPLALPAPSRTLLLAFLLLLILLGTL

Target ORF information:

RefSeq Version	NM_146227.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus protease, serine 50 (Prss50), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146227.4

ORF Insert Sequence:

ATGGAGCCCT GGTGCGGGGC AGAGGTCCGT GGGCAGGGCC CTCAGGGTCC CCGTGTGCCT 
GGGGCTTCCC GCTCCCGCTC CCGCGCCCTT CTCCTGCTGT TGCTGCTGCT GCTGCTGCTC 
CTGCCTCGGC GGCCGGCAGG TGAGCGCATC CGCCCCCGAC GCCCTCCCCG GCACGCCCAC 
CCGCGGCCAC CTCTGACTCG CTGGAGACCT TCCACAGGCT ACTTGGCCGC AGGAGCATCC 
CCGGGGACGC TGTCCACCAC CGTCCCGACC GGACCCGGGG TCTCCTGTGG CTCCAGAGGC 
ATCTGTCCCT CAGGCAGGCT TCGCCTTCCT CGGCAAGCCC AGACTAACCA GACCACGACG 
GCTCCACCGA ACTCACAGAC CATGGCTCCA CTGAAAACGG TGGGGACTCT CGGCATGATG 
GACACTACTG GGTCCGTTCT TAAGACGGTT CATTCCAGCA ACCTCCCCTT CTGTGGCTCC 
TCCCACGAGC CAGACCCTAC TCTCAGGGAC CCAGAAGCCA TGACTCGGCG GTGGCCCTGG 
ATGGTCAGCG TGCAGGCTAA TGGCTCACAC ATCTGTGCTG GCATCCTTAT CGCTTCCCAG 
TGGGTGCTGA CCGTGGCCCA TTGCTTGAGC CAGAACCATG TTAACTACAT AGTGAGGGCG 
GGGAGCCCGT GGATTAATCA GACGGCAGGA ACCAGCTCAG ATGTGCCGGT CCATCGAGTC 
ATCATAAACC ATGGCTACCA ACCCAGACGG TACTGGTCAT GGGTTGGCCG GGCCCATGAC 
ATCGGCCTTC TCAAGCTCAA GTGGGGGCTC AAGTACAGTA AATACGTGTG GCCCATCTGC 
CTGCCTGGCC TGGATTACGT GGTGGAGGAC AGTTCTCTCT GCACTGTGAC AGGCTGGGGA 
TATCCCAGGG CTAACGGCAT CTGGCCCCAG TTCCAGTCCC TCCAGGAGAA GGAAGTCTCT 
ATCCTGAACA GCAAGAAATG TGATCATTTC TACCACAAGT TCTCCAGAAT CTCCTCTCTG 
GTTCGGATCA TCAACCCTCA GATGATCTGT GCCTCGGACA ACAACAGGGA GGAGTTCTGC 
TATGAGATAA CTGGCGAGCC CCTGGTCTGC TCTTCAGATG GCACATGGTA CCTGGTGGGA 
ATGATGAGCT GGGGCCCAGG CTGCAAGAAG AGCGAGGCCC CACCCATCTT TCTGCAGGTC 
TCCTACTACA GGCCCTGGAT CTGGGACCGG CTCAGTGGGG AGCCCCTGGC CCTTCCAGCC 
CCATCCAGGA CCTTGCTCCT GGCTTTCCTT CTGCTCCTCA TCCTTCTGGG CACACTGTGA