Spg7 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spg7
Entrez Gene ID	234847
Full Name	SPG7, paraplegin matrix AAA peptidase subunit
Synonyms	AI452278,AU015315,Cmar,PGN
General protein information	Preferred Names SPG7, paraplegin matrix AAA peptidase subunit Names paraplegin cell adhesion regulator cell matrix adhesion regulator spastic paraplegia 7 homolog spastic paraplegia 7 protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_153176.4	NP_694816.3	paraplegin
NM_153176.5	NP_694816.3	paraplegin isoform 1 preproprotein
NM_001364435.1	NP_001351364.1	paraplegin isoform 2 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SPG7	XP_001140249.3
	SPG7	XP_002802648.1
Rattus norvegicus (Norway rat)	Spg7	NP_852053.1
Danio rerio (zebrafish)	spg7	XP_001923118.1
Gallus gallus (chicken)	SPG7	NP_001012545.1
Drosophila melanogaster (fruit fly)	CG2658	NP_570017.1
Caenorhabditis elegans (roundworm)	ppgn-1	NP_500191.3
Mus musculus (house mouse)	Spg7	NP_694816.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000560	XP_310523.5
Homo sapiens (human)	SPG7	NP_003110.1
Canis lupus familiaris (dog)	SPG7	XP_546777.3
Bos taurus (cattle)	SPG7	NP_001095492.1
Xenopus tropicalis (tropical clawed frog)	spg7	XP_002933705.1

Gene Ontology
Bibliography

Related articles in PubMed

CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs.
Schatton D, Pla-Martin D, Marx MC, Hansen H, Mourier A, Nemazanyy I, Pessia A, Zentis P, Corona T, Kondylis V, Barth E, Schauss AC, Velagapudi V, Rugarli EI
The Journal of cell biology216(3)675-693(2017 Mar)

Alternative splicing of Spg7, a gene involved in hereditary spastic paraplegia, encodes a variant of paraplegin targeted to the endoplasmic reticulum.
Mancuso G, Barth E, Crivello P, Rugarli EI
PloS one7(5)e36337(2012)

Autocatalytic processing of m-AAA protease subunits in mitochondria.
Koppen M, Bonn F, Ehses S, Langer T
Molecular biology of the cell20(19)4216-24(2009 Oct)

Genetic interaction between the m-AAA protease isoenzymes reveals novel roles in cerebellar degeneration.
Martinelli P, La Mattina V, Bernacchia A, Magnoni R, Cerri F, Cox G, Quattrini A, Casari G, Rugarli EI
Human molecular genetics18(11)2001-13(2009 Jun)

OPA1 processing reconstituted in yeast depends on the subunit composition of the m-AAA protease in mitochondria.
Duvezin-Caubet S, Koppen M, Wagener J, Zick M, Israel L, Bernacchia A, Jagasia R, Rugarli EI, Imhof A, Neupert W, Langer T, Reichert AS
Molecular biology of the cell18(9)3582-90(2007 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies indicate that both mouse and human SPG7 ESTs containing alternative first exons.
Title: Alternative splicing of Spg7, a gene involved in hereditary spastic paraplegia, encodes a variant of paraplegin targeted to the endoplasmic reticulum.

Data show that Afg3l1 or Afg3l2 are required for maturation of newly imported paraplegin subunits after their cleavage by MPP.
Title: Autocatalytic processing of m-AAA protease subunits in mitochondria.

Spg7 and Afg3l2 double mutants show an early-onset ataxic phenotype, indicating a role of the m-AAA proteases in cerebellar degeneration.
Title: Genetic interaction between the m-AAA protease isoenzymes reveals novel roles in cerebellar degeneration.

These results provide evidence for different substrate specificities of m-AAA proteases and reveal a striking evolutionary switch of proteases involved in the proteolytic processing of dynamin-like GTPases in mitochondria.
Title: OPA1 processing reconstituted in yeast depends on the subunit composition of the m-AAA protease in mitochondria.

The following Spg7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spg7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu84226	NM_001364435.1 Latest version!	Mus musculus SPG7, paraplegin matrix AAA peptidase subunit (Spg7), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu13086	NM_153176.5 Latest version!	Mus musculus SPG7, paraplegin matrix AAA peptidase subunit (Spg7), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
View Old Accession Versions >>
OMu13086	NM_153176.4	Mus musculus SPG7, paraplegin matrix AAA peptidase subunit (Spg7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spg7 ( NM_001364435.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001351364.1 Mus musculus SPG7, paraplegin matrix AAA peptidase subunit (Spg7), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu84226
Clone ID Related Accession (Same CDS sequence)	NM_001364435.1
Accession Version	NM_001364435.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	paraplegin isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC121819.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCG CCTTGCTGCT GCTTCGCGGG CTCCGCCCCG GCCCGGAGCC CCGGCCCCGA 
CGGCTGTGGG GCCTGCTCTC AGGCAGGGGC CCAGGCCTCT CGTCCGGGGC TGGGGCCAGG 
CGGCCGTACG CGGCCCGCGG GACTCCGGTT GGCCCGGCTG CGGCTGGAGG CCACGCGCCT 
CAGAGTTTGC TATTGCGAAT ACTGACCCCT AGCTTTGAAG GTATTAGCGG ATTGTTACTG 
AAACAGCATA TAGTTCCAAA TGCAGTCAGA CTGTGGCCGC TTTCAGGTAG CACTTTGTAT 
TTTAACACCT CAAGGATGAA GCAGAAGAAT AAAGACAATG ATAAACCCAA AGGCAAGACC 
CCTGAAGATG ATGAAGAAGA GAAGAGACGA AAAGAGCGGG AAGACCAGAT GTACCGAGAG 
AGGCTGCGCA CCCTGTTCAT CATTGCTCTT GTCATGAGCC TGCTGAACTC CCTCAGTACA 
AGTGGGGGCA GTATTTCCTG GGCTGACTTT GTCAACGAGA TGCTGGCTAA AGGCGAGGTG 
CAGCGTGTGC AGGTGGTGCC CGAGAGTGAT GTGGTGGAAG TCTATCTGCA TCCTGGAGCT 
GTGGTGTTTG GGCGGCCTCG GCTGGCCCTG ATGTATCGGA TGCAGGTTGC AAACATCGAC 
AAATTTGAAG AGAAGCTTCG AGCAGCCGAA GATGAACTGA ACATTGAGAG CAAGGACAGG 
ATTCCCGTGT CCTACAAGCG GACAGGATTC TTTGGGAATG CCCTCTACGC CCTGGGGATG 
ACAGCCGTGG GCTTGGCCAT CCTGTGGTAT GTTTTCAGAC TGGCGGGGAT GACCGGAAGG 
GAAGGCGGAT TCAGTGCTTT TAATCAGCTT AAGATGGCAC GTTTCACCAT TGTGGACGGG 
AAGACAGGGA AAGGAGTCAG CTTCCAAGAT GTGGCAGGAA TGCATGAAGC CAAGCTGGAA 
GTCCGAGAAT TTGTGGATTA TCTGAAGAGC CCAGAGCGTT TCCTTCAGCT CGGTGCCAAG 
GTTCCAAAGG GTGCCCTGTT GCTGGGGCCC CCTGGCTGTG GGAAGACGCT GTTGGCCAAG 
GCAGTAGCCA CGGAGGCTCA GGTGCCCTTT TTAGCAATGG CTGGCCCAGA GTTTGTGGAG 
GTGATTGGAG GCCTGGGAGC TGCCCGAGTG CGAAGCCTCT TCAAGGAGGC ACGAGCCAGG 
GCCCCTTGCA TAGTGTACAT TGATGAGATC GATGCTGTGG GAAAGAAGCG CTCCACCTCC 
ATGTCTGGGT TCTCCAACAC GGAAGAGGAG CAGACCCTCA ACCAGCTCCT GGTGGAGATG 
GACGGAATGG GCACCACAGA CCATGTCATC GTCTTGGCAT CCACCAATCG AGCTGATGTT 
CTGGACAATG CTCTGATGAG GCCTGGGCGG CTCGACAGGC ATGTCTTCAT TGATCTTCCC 
ACGCTCCAGG AGAGGCGGGA GATTTTCGAG CAGCACCTGA AAGGCCTGAA GTTGACCCAG 
CCCAGCAGTT TTTACTCCCA GCGGCTGGCA GAGCTGACAC CTGGATTCAG TGGCGCCGAC 
ATCGCCAACA TCTGCAACGA GGCCGCTCTG CACGCTGCAC GCGAGGGGCA CACGTCCGTG 
CACACGTTCA ACTTTGAGTA TGCCGTGGAG CGCGTCATTG CTGGTACTGC TAAAAAGAGT 
AAGATCCTCT CCAAGGAGGA GCAGAGAGTG GTCGCCTTCC ATGAGTCTGG CCATGCCTTG 
GTTGGTTGGC TGCTGGAGCA CACAGAGGCT GTGATGAAGG TCTCCATCGC ACCTCGGACA 
AACGCTGCTT TGGGCTTCTC TCAGATGCTC CCTCGGGACC AGTACCTCTT CACCAAGGAG 
CAGCTGTTTG AGCGCATGTG CATGGCCCTA GGTGGCCGTG CAGCTGAAGC CATCTCATTC 
AGCAGGGTCA CCTCTGGAAG CAAAGCTGCT GGTGGCCAAA GCCTACAGAC ACACCGAGAA 
GGTGCTGCTA GACAACCTGG ACAAGCTGCA GGCGTTGGCG AATGCCCTTC TGGAAAAGGA 
AGTGATAAAC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001351364.1
CDS	20..2074
Translation	MAAALLLLRGLRPGPEPRPRRLWGLLSGRGPGLSSGAGARRPYAARGTPVGPAAAGGHAPQSLLLRILTPSFEGISGLLLKQHIVPNAVRLWPLSGSTLYFNTSRMKQKNKDNDKPKGKTPEDDEEEKRRKEREDQMYRERLRTLFIIALVMSLLNSLSTSGGSISWADFVNEMLAKGEVQRVQVVPESDVVEVYLHPGAVVFGRPRLALMYRMQVANIDKFEEKLRAAEDELNIESKDRIPVSYKRTGFFGNALYALGMTAVGLAILWYVFRLAGMTGREGGFSAFNQLKMARFTIVDGKTGKGVSFQDVAGMHEAKLEVREFVDYLKSPERFLQLGAKVPKGALLLGPPGCGKTLLAKAVATEAQVPFLAMAGPEFVEVIGGLGAARVRSLFKEARARAPCIVYIDEIDAVGKKRSTSMSGFSNTEEEQTLNQLLVEMDGMGTTDHVIVLASTNRADVLDNALMRPGRLDRHVFIDLPTLQERREIFEQHLKGLKLTQPSSFYSQRLAELTPGFSGADIANICNEAALHAAREGHTSVHTFNFEYAVERVIAGTAKKSKILSKEEQRVVAFHESGHALVGWLLEHTEAVMKVSIAPRTNAALGFSQMLPRDQYLFTKEQLFERMCMALGGRAAEAISFSRVTSGSKAAGGQSLQTHREGAARQPGQAAGVGECPSGKGSDKL

Target ORF information:

RefSeq Version	NM_001364435.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPG7, paraplegin matrix AAA peptidase subunit (Spg7), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001364435.1

ORF Insert Sequence:

ATGGCGGCCG CCTTGCTGCT GCTTCGCGGG CTCCGCCCCG GCCCGGAGCC CCGGCCCCGA 
CGGCTGTGGG GCCTGCTCTC AGGCAGGGGC CCAGGCCTCT CGTCCGGGGC TGGGGCCAGG 
CGGCCGTACG CGGCCCGCGG GACTCCGGTT GGCCCGGCTG CGGCTGGAGG CCACGCGCCT 
CAGAGTTTGC TATTGCGAAT ACTGACCCCT AGCTTTGAAG GTATTAGCGG ATTGTTACTG 
AAACAGCATA TAGTTCCAAA TGCAGTCAGA CTGTGGCCGC TTTCAGGTAG CACTTTGTAT 
TTTAACACCT CAAGGATGAA GCAGAAGAAT AAAGACAATG ATAAACCCAA AGGCAAGACC 
CCTGAAGATG ATGAAGAAGA GAAGAGACGA AAAGAGCGGG AAGACCAGAT GTACCGAGAG 
AGGCTGCGCA CCCTGTTCAT CATTGCTCTT GTCATGAGCC TGCTGAACTC CCTCAGTACA 
AGTGGGGGCA GTATTTCCTG GGCTGACTTT GTCAACGAGA TGCTGGCTAA AGGCGAGGTG 
CAGCGTGTGC AGGTGGTGCC CGAGAGTGAT GTGGTGGAAG TCTATCTGCA TCCTGGAGCT 
GTGGTGTTTG GGCGGCCTCG GCTGGCCCTG ATGTATCGGA TGCAGGTTGC AAACATCGAC 
AAATTTGAAG AGAAGCTTCG AGCAGCCGAA GATGAACTGA ACATTGAGAG CAAGGACAGG 
ATTCCCGTGT CCTACAAGCG GACAGGATTC TTTGGGAATG CCCTCTACGC CCTGGGGATG 
ACAGCCGTGG GCTTGGCCAT CCTGTGGTAT GTTTTCAGAC TGGCGGGGAT GACCGGAAGG 
GAAGGCGGAT TCAGTGCTTT TAATCAGCTT AAGATGGCAC GTTTCACCAT TGTGGACGGG 
AAGACAGGGA AAGGAGTCAG CTTCCAAGAT GTGGCAGGAA TGCATGAAGC CAAGCTGGAA 
GTCCGAGAAT TTGTGGATTA TCTGAAGAGC CCAGAGCGTT TCCTTCAGCT CGGTGCCAAG 
GTTCCAAAGG GTGCCCTGTT GCTGGGGCCC CCTGGCTGTG GGAAGACGCT GTTGGCCAAG 
GCAGTAGCCA CGGAGGCTCA GGTGCCCTTT TTAGCAATGG CTGGCCCAGA GTTTGTGGAG 
GTGATTGGAG GCCTGGGAGC TGCCCGAGTG CGAAGCCTCT TCAAGGAGGC ACGAGCCAGG 
GCCCCTTGCA TAGTGTACAT TGATGAGATC GATGCTGTGG GAAAGAAGCG CTCCACCTCC 
ATGTCTGGGT TCTCCAACAC GGAAGAGGAG CAGACCCTCA ACCAGCTCCT GGTGGAGATG 
GACGGAATGG GCACCACAGA CCATGTCATC GTCTTGGCAT CCACCAATCG AGCTGATGTT 
CTGGACAATG CTCTGATGAG GCCTGGGCGG CTCGACAGGC ATGTCTTCAT TGATCTTCCC 
ACGCTCCAGG AGAGGCGGGA GATTTTCGAG CAGCACCTGA AAGGCCTGAA GTTGACCCAG 
CCCAGCAGTT TTTACTCCCA GCGGCTGGCA GAGCTGACAC CTGGATTCAG TGGCGCCGAC 
ATCGCCAACA TCTGCAACGA GGCCGCTCTG CACGCTGCAC GCGAGGGGCA CACGTCCGTG 
CACACGTTCA ACTTTGAGTA TGCCGTGGAG CGCGTCATTG CTGGTACTGC TAAAAAGAGT 
AAGATCCTCT CCAAGGAGGA GCAGAGAGTG GTCGCCTTCC ATGAGTCTGG CCATGCCTTG 
GTTGGTTGGC TGCTGGAGCA CACAGAGGCT GTGATGAAGG TCTCCATCGC ACCTCGGACA 
AACGCTGCTT TGGGCTTCTC TCAGATGCTC CCTCGGGACC AGTACCTCTT CACCAAGGAG 
CAGCTGTTTG AGCGCATGTG CATGGCCCTA GGTGGCCGTG CAGCTGAAGC CATCTCATTC 
AGCAGGGTCA CCTCTGGAAG CAAAGCTGCT GGTGGCCAAA GCCTACAGAC ACACCGAGAA 
GGTGCTGCTA GACAACCTGG ACAAGCTGCA GGCGTTGGCG AATGCCCTTC TGGAAAAGGA 
AGTGATAAAC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spg7 ( NM_153176.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_694816.3 Mus musculus SPG7, paraplegin matrix AAA peptidase subunit (Spg7), mRNA.

Price & Availability↑

CloneID	OMu13086
Clone ID Related Accession (Same CDS sequence)	NM_153176.4 , NM_153176.5
Accession Version	NM_153176.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Mus musculus(house mouse)
Product	paraplegin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK145540.1. On Jun 3, 2007 this sequence version replaced NM_153176.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK145540.1, AF512565.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGCGGCCG CCTTGCTGCT GCTTCGCGGG CTCCGCCCCG GCCCGGAGCC CCGGCCCCGA 
CGGCTGTGGG GCCTGCTCTC AGGCAGGGGC CCAGGCCTCT CGTCCGGGGC TGGGGCCAGG 
CGGCCGTACG CGGCCCGCGG GACTCCGGTT GGCCCGGCTG CGGCTGGAGG CCACGCGCCT 
CAGAGTTTGC TATTGCGAAT ACTGACCCCT AGCTTTGAAG GTATTAGCGG ATTGTTACTG 
AAACAGCATA TAGTTCCAAA TGCAGTCAGA CTGTGGCCGC TTTCAGGTAG CACTTTGTAT 
TTTAACACCT CAAGGATGAA GCAGAAGAAT AAAGACAATG ATAAACCCAA AGGCAAGACC 
CCTGAAGATG ATGAAGAAGA GAAGAGACGA AAAGAGCGGG AAGACCAGAT GTACCGAGAG 
AGGCTGCGCA CCCTGTTCAT CATTGCTCTT GTCATGAGCC TGCTGAACTC CCTCAGTACA 
AGTGGGGGCA GTATTTCCTG GGCTGACTTT GTCAACGAGA TGCTGGCTAA AGGCGAGGTG 
CAGCGTGTGC AGGTGGTGCC CGAGAGTGAT GTGGTGGAAG TCTATCTGCA TCCTGGAGCT 
GTGGTGTTTG GGCGGCCTCG GCTGGCCCTG ATGTATCGGA TGCAGGTTGC AAACATCGAC 
AAATTTGAAG AGAAGCTTCG AGCAGCCGAA GATGAACTGA ACATTGAGAG CAAGGACAGG 
ATTCCCGTGT CCTACAAGCG GACAGGATTC TTTGGGAATG CCCTCTACGC CCTGGGGATG 
ACAGCCGTGG GCTTGGCCAT CCTGTGGTAT GTTTTCAGAC TGGCGGGGAT GACCGGAAGG 
GAAGGCGGAT TCAGTGCTTT TAATCAGCTT AAGATGGCAC GTTTCACCAT TGTGGACGGG 
AAGACAGGGA AAGGAGTCAG CTTCCAAGAT GTGGCAGGAA TGCATGAAGC CAAGCTGGAA 
GTCCGAGAAT TTGTGGATTA TCTGAAGAGC CCAGAGCGTT TCCTTCAGCT CGGTGCCAAG 
GTTCCAAAGG GTGCCCTGTT GCTGGGGCCC CCTGGCTGTG GGAAGACGCT GTTGGCCAAG 
GCAGTAGCCA CGGAGGCTCA GGTGCCCTTT TTAGCAATGG CTGGCCCAGA GTTTGTGGAG 
GTGATTGGAG GCCTGGGAGC TGCCCGAGTG CGAAGCCTCT TCAAGGAGGC ACGAGCCAGG 
GCCCCTTGCA TAGTGTACAT TGATGAGATC GATGCTGTGG GAAAGAAGCG CTCCACCTCC 
ATGTCTGGGT TCTCCAACAC GGAAGAGGAG CAGACCCTCA ACCAGCTCCT GGTGGAGATG 
GACGGAATGG GCACCACAGA CCATGTCATC GTCTTGGCAT CCACCAATCG AGCTGATGTT 
CTGGACAATG CTCTGATGAG GCCTGGGCGG CTCGACAGGC ATGTCTTCAT TGATCTTCCC 
ACGCTCCAGG AGAGGCGGGA GATTTTCGAG CAGCACCTGA AAGGCCTGAA GTTGACCCAG 
CCCAGCAGTT TTTACTCCCA GCGGCTGGCA GAGCTGACAC CTGGATTCAG TGGCGCCGAC 
ATCGCCAACA TCTGCAACGA GGCCGCTCTG CACGCTGCAC GCGAGGGGCA CACGTCCGTG 
CACACGTTCA ACTTTGAGTA TGCCGTGGAG CGCGTCATTG CTGGTACTGC TAAAAAGAGT 
AAGATCCTCT CCAAGGAGGA GCAGAGAGTG GTCGCCTTCC ATGAGTCTGG CCATGCCTTG 
GTTGGTTGGC TGCTGGAGCA CACAGAGGCT GTGATGAAGG TCTCCATCGC ACCTCGGACA 
AACGCTGCTT TGGGCTTCTC TCAGATGCTC CCTCGGGACC AGTACCTCTT CACCAAGGAG 
CAGCTGTTTG AGCGCATGTG CATGGCCCTA GGTGGCCGTG CAGCTGAAGC CATCTCATTC 
AGCAGGGTCA CCTCTGGGGC CCAGGATGAT TTGAGGAAGG TTACCCGCAT TGCTTACTCC 
ATGGTGAAAC AGTTCGGGAT GGCTCCCAGC ATTGGGCCTG TATCCTTCCC CGAGGCACAA 
GAGGGCCTCA TGGGCATTGG ACGTCGTCCC TTCAGCCAGG GCCTCCAGCA GATGATGGAC 
CATGAAGCAA AGCTGCTGGT GGCCAAAGCC TACAGACACA CCGAGAAGGT GCTGCTAGAC 
AACCTGGACA AGCTGCAGGC GTTGGCGAAT GCCCTTCTGG AAAAGGAAGT GATAAACTAT 
GAGGACATTG AGGCGCTCAT TGGCCCGCCG CCCCATGGGC CAAAGAAAAT GATTGCACCA 
CAGAAATGGA TTGATGCTGA AAAGGAGAGA CAGGCCTCAG GGGAGGAGGA GGCTCCGGCT 
CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_694816.3
CDS	20..2365
Translation	MAAALLLLRGLRPGPEPRPRRLWGLLSGRGPGLSSGAGARRPYAARGTPVGPAAAGGHAPQSLLLRILTPSFEGISGLLLKQHIVPNAVRLWPLSGSTLYFNTSRMKQKNKDNDKPKGKTPEDDEEEKRRKEREDQMYRERLRTLFIIALVMSLLNSLSTSGGSISWADFVNEMLAKGEVQRVQVVPESDVVEVYLHPGAVVFGRPRLALMYRMQVANIDKFEEKLRAAEDELNIESKDRIPVSYKRTGFFGNALYALGMTAVGLAILWYVFRLAGMTGREGGFSAFNQLKMARFTIVDGKTGKGVSFQDVAGMHEAKLEVREFVDYLKSPERFLQLGAKVPKGALLLGPPGCGKTLLAKAVATEAQVPFLAMAGPEFVEVIGGLGAARVRSLFKEARARAPCIVYIDEIDAVGKKRSTSMSGFSNTEEEQTLNQLLVEMDGMGTTDHVIVLASTNRADVLDNALMRPGRLDRHVFIDLPTLQERREIFEQHLKGLKLTQPSSFYSQRLAELTPGFSGADIANICNEAALHAAREGHTSVHTFNFEYAVERVIAGTAKKSKILSKEEQRVVAFHESGHALVGWLLEHTEAVMKVSIAPRTNAALGFSQMLPRDQYLFTKEQLFERMCMALGGRAAEAISFSRVTSGAQDDLRKVTRIAYSMVKQFGMAPSIGPVSFPEAQEGLMGIGRRPFSQGLQQMMDHEAKLLVAKAYRHTEKVLLDNLDKLQALANALLEKEVINYEDIEALIGPPPHGPKKMIAPQKWIDAEKERQASGEEEAPAP

Target ORF information:

RefSeq Version	NM_153176.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPG7, paraplegin matrix AAA peptidase subunit (Spg7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153176.4

ORF Insert Sequence:

ATGGCGGCCG CCTTGCTGCT GCTTCGCGGG CTCCGCCCCG GCCCGGAGCC CCGGCCCCGA 
CGGCTGTGGG GCCTGCTCTC AGGCAGGGGC CCAGGCCTCT CGTCCGGGGC TGGGGCCAGG 
CGGCCGTACG CGGCCCGCGG GACTCCGGTT GGCCCGGCTG CGGCTGGAGG CCACGCGCCT 
CAGAGTTTGC TATTGCGAAT ACTGACCCCT AGCTTTGAAG GTATTAGCGG ATTGTTACTG 
AAACAGCATA TAGTTCCAAA TGCAGTCAGA CTGTGGCCGC TTTCAGGTAG CACTTTGTAT 
TTTAACACCT CAAGGATGAA GCAGAAGAAT AAAGACAATG ATAAACCCAA AGGCAAGACC 
CCTGAAGATG ATGAAGAAGA GAAGAGACGA AAAGAGCGGG AAGACCAGAT GTACCGAGAG 
AGGCTGCGCA CCCTGTTCAT CATTGCTCTT GTCATGAGCC TGCTGAACTC CCTCAGTACA 
AGTGGGGGCA GTATTTCCTG GGCTGACTTT GTCAACGAGA TGCTGGCTAA AGGCGAGGTG 
CAGCGTGTGC AGGTGGTGCC CGAGAGTGAT GTGGTGGAAG TCTATCTGCA TCCTGGAGCT 
GTGGTGTTTG GGCGGCCTCG GCTGGCCCTG ATGTATCGGA TGCAGGTTGC AAACATCGAC 
AAATTTGAAG AGAAGCTTCG AGCAGCCGAA GATGAACTGA ACATTGAGAG CAAGGACAGG 
ATTCCCGTGT CCTACAAGCG GACAGGATTC TTTGGGAATG CCCTCTACGC CCTGGGGATG 
ACAGCCGTGG GCTTGGCCAT CCTGTGGTAT GTTTTCAGAC TGGCGGGGAT GACCGGAAGG 
GAAGGCGGAT TCAGTGCTTT TAATCAGCTT AAGATGGCAC GTTTCACCAT TGTGGACGGG 
AAGACAGGGA AAGGAGTCAG CTTCCAAGAT GTGGCAGGAA TGCATGAAGC CAAGCTGGAA 
GTCCGAGAAT TTGTGGATTA TCTGAAGAGC CCAGAGCGTT TCCTTCAGCT CGGTGCCAAG 
GTTCCAAAGG GTGCCCTGTT GCTGGGGCCC CCTGGCTGTG GGAAGACGCT GTTGGCCAAG 
GCAGTAGCCA CGGAGGCTCA GGTGCCCTTT TTAGCAATGG CTGGCCCAGA GTTTGTGGAG 
GTGATTGGAG GCCTGGGAGC TGCCCGAGTG CGAAGCCTCT TCAAGGAGGC ACGAGCCAGG 
GCCCCTTGCA TAGTGTACAT TGATGAGATC GATGCTGTGG GAAAGAAGCG CTCCACCTCC 
ATGTCTGGGT TCTCCAACAC GGAAGAGGAG CAGACCCTCA ACCAGCTCCT GGTGGAGATG 
GACGGAATGG GCACCACAGA CCATGTCATC GTCTTGGCAT CCACCAATCG AGCTGATGTT 
CTGGACAATG CTCTGATGAG GCCTGGGCGG CTCGACAGGC ATGTCTTCAT TGATCTTCCC 
ACGCTCCAGG AGAGGCGGGA GATTTTCGAG CAGCACCTGA AAGGCCTGAA GTTGACCCAG 
CCCAGCAGTT TTTACTCCCA GCGGCTGGCA GAGCTGACAC CTGGATTCAG TGGCGCCGAC 
ATCGCCAACA TCTGCAACGA GGCCGCTCTG CACGCTGCAC GCGAGGGGCA CACGTCCGTG 
CACACGTTCA ACTTTGAGTA TGCCGTGGAG CGCGTCATTG CTGGTACTGC TAAAAAGAGT 
AAGATCCTCT CCAAGGAGGA GCAGAGAGTG GTCGCCTTCC ATGAGTCTGG CCATGCCTTG 
GTTGGTTGGC TGCTGGAGCA CACAGAGGCT GTGATGAAGG TCTCCATCGC ACCTCGGACA 
AACGCTGCTT TGGGCTTCTC TCAGATGCTC CCTCGGGACC AGTACCTCTT CACCAAGGAG 
CAGCTGTTTG AGCGCATGTG CATGGCCCTA GGTGGCCGTG CAGCTGAAGC CATCTCATTC 
AGCAGGGTCA CCTCTGGGGC CCAGGATGAT TTGAGGAAGG TTACCCGCAT TGCTTACTCC 
ATGGTGAAAC AGTTCGGGAT GGCTCCCAGC ATTGGGCCTG TATCCTTCCC CGAGGCACAA 
GAGGGCCTCA TGGGCATTGG ACGTCGTCCC TTCAGCCAGG GCCTCCAGCA GATGATGGAC 
CATGAAGCAA AGCTGCTGGT GGCCAAAGCC TACAGACACA CCGAGAAGGT GCTGCTAGAC 
AACCTGGACA AGCTGCAGGC GTTGGCGAAT GCCCTTCTGG AAAAGGAAGT GATAAACTAT 
GAGGACATTG AGGCGCTCAT TGGCCCGCCG CCCCATGGGC CAAAGAAAAT GATTGCACCA 
CAGAAATGGA TTGATGCTGA AAAGGAGAGA CAGGCCTCAG GGGAGGAGGA GGCTCCGGCT 
CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spg7 ( NM_153176.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_694816.3 Mus musculus SPG7, paraplegin matrix AAA peptidase subunit (Spg7), transcript variant 1, mRNA.

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CloneID	OMu13086
Clone ID Related Accession (Same CDS sequence)	NM_153176.4 , NM_153176.5
Accession Version	NM_153176.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	paraplegin isoform 1 preproprotein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK145540.1 and BP770238.1. On Jun 16, 2018 this sequence version replaced NM_153176.4. ##Evidence-Data-START## Transcript exon combination :: AF512565.1, AF547215.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCG CCTTGCTGCT GCTTCGCGGG CTCCGCCCCG GCCCGGAGCC CCGGCCCCGA 
CGGCTGTGGG GCCTGCTCTC AGGCAGGGGC CCAGGCCTCT CGTCCGGGGC TGGGGCCAGG 
CGGCCGTACG CGGCCCGCGG GACTCCGGTT GGCCCGGCTG CGGCTGGAGG CCACGCGCCT 
CAGAGTTTGC TATTGCGAAT ACTGACCCCT AGCTTTGAAG GTATTAGCGG ATTGTTACTG 
AAACAGCATA TAGTTCCAAA TGCAGTCAGA CTGTGGCCGC TTTCAGGTAG CACTTTGTAT 
TTTAACACCT CAAGGATGAA GCAGAAGAAT AAAGACAATG ATAAACCCAA AGGCAAGACC 
CCTGAAGATG ATGAAGAAGA GAAGAGACGA AAAGAGCGGG AAGACCAGAT GTACCGAGAG 
AGGCTGCGCA CCCTGTTCAT CATTGCTCTT GTCATGAGCC TGCTGAACTC CCTCAGTACA 
AGTGGGGGCA GTATTTCCTG GGCTGACTTT GTCAACGAGA TGCTGGCTAA AGGCGAGGTG 
CAGCGTGTGC AGGTGGTGCC CGAGAGTGAT GTGGTGGAAG TCTATCTGCA TCCTGGAGCT 
GTGGTGTTTG GGCGGCCTCG GCTGGCCCTG ATGTATCGGA TGCAGGTTGC AAACATCGAC 
AAATTTGAAG AGAAGCTTCG AGCAGCCGAA GATGAACTGA ACATTGAGAG CAAGGACAGG 
ATTCCCGTGT CCTACAAGCG GACAGGATTC TTTGGGAATG CCCTCTACGC CCTGGGGATG 
ACAGCCGTGG GCTTGGCCAT CCTGTGGTAT GTTTTCAGAC TGGCGGGGAT GACCGGAAGG 
GAAGGCGGAT TCAGTGCTTT TAATCAGCTT AAGATGGCAC GTTTCACCAT TGTGGACGGG 
AAGACAGGGA AAGGAGTCAG CTTCCAAGAT GTGGCAGGAA TGCATGAAGC CAAGCTGGAA 
GTCCGAGAAT TTGTGGATTA TCTGAAGAGC CCAGAGCGTT TCCTTCAGCT CGGTGCCAAG 
GTTCCAAAGG GTGCCCTGTT GCTGGGGCCC CCTGGCTGTG GGAAGACGCT GTTGGCCAAG 
GCAGTAGCCA CGGAGGCTCA GGTGCCCTTT TTAGCAATGG CTGGCCCAGA GTTTGTGGAG 
GTGATTGGAG GCCTGGGAGC TGCCCGAGTG CGAAGCCTCT TCAAGGAGGC ACGAGCCAGG 
GCCCCTTGCA TAGTGTACAT TGATGAGATC GATGCTGTGG GAAAGAAGCG CTCCACCTCC 
ATGTCTGGGT TCTCCAACAC GGAAGAGGAG CAGACCCTCA ACCAGCTCCT GGTGGAGATG 
GACGGAATGG GCACCACAGA CCATGTCATC GTCTTGGCAT CCACCAATCG AGCTGATGTT 
CTGGACAATG CTCTGATGAG GCCTGGGCGG CTCGACAGGC ATGTCTTCAT TGATCTTCCC 
ACGCTCCAGG AGAGGCGGGA GATTTTCGAG CAGCACCTGA AAGGCCTGAA GTTGACCCAG 
CCCAGCAGTT TTTACTCCCA GCGGCTGGCA GAGCTGACAC CTGGATTCAG TGGCGCCGAC 
ATCGCCAACA TCTGCAACGA GGCCGCTCTG CACGCTGCAC GCGAGGGGCA CACGTCCGTG 
CACACGTTCA ACTTTGAGTA TGCCGTGGAG CGCGTCATTG CTGGTACTGC TAAAAAGAGT 
AAGATCCTCT CCAAGGAGGA GCAGAGAGTG GTCGCCTTCC ATGAGTCTGG CCATGCCTTG 
GTTGGTTGGC TGCTGGAGCA CACAGAGGCT GTGATGAAGG TCTCCATCGC ACCTCGGACA 
AACGCTGCTT TGGGCTTCTC TCAGATGCTC CCTCGGGACC AGTACCTCTT CACCAAGGAG 
CAGCTGTTTG AGCGCATGTG CATGGCCCTA GGTGGCCGTG CAGCTGAAGC CATCTCATTC 
AGCAGGGTCA CCTCTGGGGC CCAGGATGAT TTGAGGAAGG TTACCCGCAT TGCTTACTCC 
ATGGTGAAAC AGTTCGGGAT GGCTCCCAGC ATTGGGCCTG TATCCTTCCC CGAGGCACAA 
GAGGGCCTCA TGGGCATTGG ACGTCGTCCC TTCAGCCAGG GCCTCCAGCA GATGATGGAC 
CATGAAGCAA AGCTGCTGGT GGCCAAAGCC TACAGACACA CCGAGAAGGT GCTGCTAGAC 
AACCTGGACA AGCTGCAGGC GTTGGCGAAT GCCCTTCTGG AAAAGGAAGT GATAAACTAT 
GAGGACATTG AGGCGCTCAT TGGCCCGCCG CCCCATGGGC CAAAGAAAAT GATTGCACCA 
CAGAAATGGA TTGATGCTGA AAAGGAGAGA CAGGCCTCAG GGGAGGAGGA GGCTCCGGCT 
CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_694816.3
CDS	20..2365
Translation	MAAALLLLRGLRPGPEPRPRRLWGLLSGRGPGLSSGAGARRPYAARGTPVGPAAAGGHAPQSLLLRILTPSFEGISGLLLKQHIVPNAVRLWPLSGSTLYFNTSRMKQKNKDNDKPKGKTPEDDEEEKRRKEREDQMYRERLRTLFIIALVMSLLNSLSTSGGSISWADFVNEMLAKGEVQRVQVVPESDVVEVYLHPGAVVFGRPRLALMYRMQVANIDKFEEKLRAAEDELNIESKDRIPVSYKRTGFFGNALYALGMTAVGLAILWYVFRLAGMTGREGGFSAFNQLKMARFTIVDGKTGKGVSFQDVAGMHEAKLEVREFVDYLKSPERFLQLGAKVPKGALLLGPPGCGKTLLAKAVATEAQVPFLAMAGPEFVEVIGGLGAARVRSLFKEARARAPCIVYIDEIDAVGKKRSTSMSGFSNTEEEQTLNQLLVEMDGMGTTDHVIVLASTNRADVLDNALMRPGRLDRHVFIDLPTLQERREIFEQHLKGLKLTQPSSFYSQRLAELTPGFSGADIANICNEAALHAAREGHTSVHTFNFEYAVERVIAGTAKKSKILSKEEQRVVAFHESGHALVGWLLEHTEAVMKVSIAPRTNAALGFSQMLPRDQYLFTKEQLFERMCMALGGRAAEAISFSRVTSGAQDDLRKVTRIAYSMVKQFGMAPSIGPVSFPEAQEGLMGIGRRPFSQGLQQMMDHEAKLLVAKAYRHTEKVLLDNLDKLQALANALLEKEVINYEDIEALIGPPPHGPKKMIAPQKWIDAEKERQASGEEEAPAP

Target ORF information:

RefSeq Version	NM_153176.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPG7, paraplegin matrix AAA peptidase subunit (Spg7), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153176.5

ORF Insert Sequence:

ATGGCGGCCG CCTTGCTGCT GCTTCGCGGG CTCCGCCCCG GCCCGGAGCC CCGGCCCCGA 
CGGCTGTGGG GCCTGCTCTC AGGCAGGGGC CCAGGCCTCT CGTCCGGGGC TGGGGCCAGG 
CGGCCGTACG CGGCCCGCGG GACTCCGGTT GGCCCGGCTG CGGCTGGAGG CCACGCGCCT 
CAGAGTTTGC TATTGCGAAT ACTGACCCCT AGCTTTGAAG GTATTAGCGG ATTGTTACTG 
AAACAGCATA TAGTTCCAAA TGCAGTCAGA CTGTGGCCGC TTTCAGGTAG CACTTTGTAT 
TTTAACACCT CAAGGATGAA GCAGAAGAAT AAAGACAATG ATAAACCCAA AGGCAAGACC 
CCTGAAGATG ATGAAGAAGA GAAGAGACGA AAAGAGCGGG AAGACCAGAT GTACCGAGAG 
AGGCTGCGCA CCCTGTTCAT CATTGCTCTT GTCATGAGCC TGCTGAACTC CCTCAGTACA 
AGTGGGGGCA GTATTTCCTG GGCTGACTTT GTCAACGAGA TGCTGGCTAA AGGCGAGGTG 
CAGCGTGTGC AGGTGGTGCC CGAGAGTGAT GTGGTGGAAG TCTATCTGCA TCCTGGAGCT 
GTGGTGTTTG GGCGGCCTCG GCTGGCCCTG ATGTATCGGA TGCAGGTTGC AAACATCGAC 
AAATTTGAAG AGAAGCTTCG AGCAGCCGAA GATGAACTGA ACATTGAGAG CAAGGACAGG 
ATTCCCGTGT CCTACAAGCG GACAGGATTC TTTGGGAATG CCCTCTACGC CCTGGGGATG 
ACAGCCGTGG GCTTGGCCAT CCTGTGGTAT GTTTTCAGAC TGGCGGGGAT GACCGGAAGG 
GAAGGCGGAT TCAGTGCTTT TAATCAGCTT AAGATGGCAC GTTTCACCAT TGTGGACGGG 
AAGACAGGGA AAGGAGTCAG CTTCCAAGAT GTGGCAGGAA TGCATGAAGC CAAGCTGGAA 
GTCCGAGAAT TTGTGGATTA TCTGAAGAGC CCAGAGCGTT TCCTTCAGCT CGGTGCCAAG 
GTTCCAAAGG GTGCCCTGTT GCTGGGGCCC CCTGGCTGTG GGAAGACGCT GTTGGCCAAG 
GCAGTAGCCA CGGAGGCTCA GGTGCCCTTT TTAGCAATGG CTGGCCCAGA GTTTGTGGAG 
GTGATTGGAG GCCTGGGAGC TGCCCGAGTG CGAAGCCTCT TCAAGGAGGC ACGAGCCAGG 
GCCCCTTGCA TAGTGTACAT TGATGAGATC GATGCTGTGG GAAAGAAGCG CTCCACCTCC 
ATGTCTGGGT TCTCCAACAC GGAAGAGGAG CAGACCCTCA ACCAGCTCCT GGTGGAGATG 
GACGGAATGG GCACCACAGA CCATGTCATC GTCTTGGCAT CCACCAATCG AGCTGATGTT 
CTGGACAATG CTCTGATGAG GCCTGGGCGG CTCGACAGGC ATGTCTTCAT TGATCTTCCC 
ACGCTCCAGG AGAGGCGGGA GATTTTCGAG CAGCACCTGA AAGGCCTGAA GTTGACCCAG 
CCCAGCAGTT TTTACTCCCA GCGGCTGGCA GAGCTGACAC CTGGATTCAG TGGCGCCGAC 
ATCGCCAACA TCTGCAACGA GGCCGCTCTG CACGCTGCAC GCGAGGGGCA CACGTCCGTG 
CACACGTTCA ACTTTGAGTA TGCCGTGGAG CGCGTCATTG CTGGTACTGC TAAAAAGAGT 
AAGATCCTCT CCAAGGAGGA GCAGAGAGTG GTCGCCTTCC ATGAGTCTGG CCATGCCTTG 
GTTGGTTGGC TGCTGGAGCA CACAGAGGCT GTGATGAAGG TCTCCATCGC ACCTCGGACA 
AACGCTGCTT TGGGCTTCTC TCAGATGCTC CCTCGGGACC AGTACCTCTT CACCAAGGAG 
CAGCTGTTTG AGCGCATGTG CATGGCCCTA GGTGGCCGTG CAGCTGAAGC CATCTCATTC 
AGCAGGGTCA CCTCTGGGGC CCAGGATGAT TTGAGGAAGG TTACCCGCAT TGCTTACTCC 
ATGGTGAAAC AGTTCGGGAT GGCTCCCAGC ATTGGGCCTG TATCCTTCCC CGAGGCACAA 
GAGGGCCTCA TGGGCATTGG ACGTCGTCCC TTCAGCCAGG GCCTCCAGCA GATGATGGAC 
CATGAAGCAA AGCTGCTGGT GGCCAAAGCC TACAGACACA CCGAGAAGGT GCTGCTAGAC 
AACCTGGACA AGCTGCAGGC GTTGGCGAAT GCCCTTCTGG AAAAGGAAGT GATAAACTAT 
GAGGACATTG AGGCGCTCAT TGGCCCGCCG CCCCATGGGC CAAAGAAAAT GATTGCACCA 
CAGAAATGGA TTGATGCTGA AAAGGAGAGA CAGGCCTCAG GGGAGGAGGA GGCTCCGGCT 
CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs.
The Journal of cell biology216(3)675-693(2017 Mar)
Schatton D,Pla-Martin D,Marx MC,Hansen H,Mourier A,Nemazanyy I,Pessia A,Zentis P,Corona T,Kondylis V,Barth E,Schauss AC,Velagapudi V,Rugarli EI

Alternative splicing of Spg7, a gene involved in hereditary spastic paraplegia, encodes a variant of paraplegin targeted to the endoplasmic reticulum.
PloS one7(5)e36337(2012)
Mancuso G,Barth E,Crivello P,Rugarli EI

Autocatalytic processing of m-AAA protease subunits in mitochondria.
Molecular biology of the cell20(19)4216-24(2009 Oct)
Koppen M,Bonn F,Ehses S,Langer T

Genetic interaction between the m-AAA protease isoenzymes reveals novel roles in cerebellar degeneration.
Human molecular genetics18(11)2001-13(2009 Jun)
Martinelli P,La Mattina V,Bernacchia A,Magnoni R,Cerri F,Cox G,Quattrini A,Casari G,Rugarli EI

OPA1 processing reconstituted in yeast depends on the subunit composition of the m-AAA protease in mitochondria.
Molecular biology of the cell18(9)3582-90(2007 Sep)
Duvezin-Caubet S,Koppen M,Wagener J,Zick M,Israel L,Bernacchia A,Jagasia R,Rugarli EI,Imhof A,Neupert W,Langer T,Reichert AS

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Spg7 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

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Eメール

電話

FAX

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