ORF » Species Summary » Mus musculus » Nae1 cDNA ORF clone

Nae1 cDNA ORF clone, Mus musculus(house mouse)

The following Nae1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nae1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu15888 NM_144931.3
Latest version!		 Mus musculus NEDD8 activating enzyme E1 subunit 1 (Nae1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $321.30
$459.00
OMu41369 XM_006530884.3
Latest version!		 Mus musculus NEDD8 activating enzyme E1 subunit 1 (Nae1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OMu41369 XM_006530884.2 Mus musculus NEDD8 activating enzyme E1 subunit 1 (Nae1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    Nae1 ( XM_006530884.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530947.1 Mus musculus NEDD8 activating enzyme E1 subunit 1 (Nae1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OMu41369
    Clone ID Related Accession (Same CDS sequence) XM_006530884.3 , XM_006530884.2
    Accession Version XM_006530884.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1533bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product NEDD8-activating enzyme E1 regulatory subunit isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006530884.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGCGCAGC CAGGGAAGAT ACTCAAGGAG CAAAAGTACG ACCGGCAGCT GAGGCTGTGG 
    GGTGATCATG GACAAGAAGC TTTGGAATCT GCTCATGTTT GCTTAATAAA TGCAACGGCT 
    ACAGGAACTG AAATTCTTAA AAACTTGGTG CTGCCAGGTA TTGGATCATT TACAATTATT 
    GATGGAAATC TGGTCAGCGG AGAAGATGCT GGAAACAACT TTTTCCTTCA AAAAAGCAGT 
    ATTGGCAAGA GTCCAGAAAA CCTTCTAGAC AATGATCCTT CATTTTTCTG TAGGTTTACT 
    ATTGTGGTTG CAACTCAGCT TCTTGAAAGT ACATTGCTGC GTTTAGCAGA TGTTCTCTGG 
    AATTCCCAGA TCCCTCTTTT GATCTGTAGG ACATATGGAC TAGTTGGTTA TATGAGGATT 
    ATTATAAAAG AACACCCAGT AATAGAATCT CATCCAGATA ATGCATTGGA GGATCTACGA 
    CTAGATAAGC CATTTCCTGA ACTGAGAGAA CATCTTCAGT CTTATGACTT GGACCATATG 
    GAAAAAAAGG ACCATAGCCA TACTCCATGG ATTGTGATCA TAGCTAAATA TTTAGCACAG 
    TGGTATAATG AAACAAATGG ACGGATTCCT AAAAGTTATA AAGAAAAAGA GGACTTCAGA 
    GATTTGATAA GACAAGGGAT TTTAAAGAAT GAAAATGGAG CTCCAGAAGA CGAAGAGAAT 
    TTTGAAGAAG CCATCAAAAA TGTGAACACA GCACTAAATA CAACTCAGAT CCCAAGCAGT 
    ATTGAAGATA TATTTAATGA TGATCGATGC ATAAATATCA CCAAACAGAC ACCAACTTTT 
    TGGATTCTAG CTCGTGCCTT AAAGGAATTT GTGGCCAAGG AGGGTCAAGG AAATTTACCT 
    GTCCGAGGCA CAATTCCTGA TATGATTGCA GATTCAAACA AATACATAAA ACTGCAGAAT 
    GTTTACCGAG AGAAAGCAAA GAAAGATGCT GCTGCTGTAG GCAATCACGT TGCCAAATTG 
    CTTCAGTCTG TTGGCCAGGC ACCAGAGTCC ATTTCAGAGA AAGAATTAAA ATTGCTTTGC 
    AGCAATTCTG CATTTCTTCG AGTGGTAAGA TGTCGATCTT TAGCTGAAGA GTATGGCTTG 
    GATACAGTCA ACAAAGATGA AATTATTTCT AGCATGGACA ATCCGGATAA TGAGATAGTC 
    CTGTACCTGA TGTTGCGGGC TGTTGATCGA TTTCATAAAC AGCATGGCAG ATACCCAGGA 
    GTATCTAACT ATCAAGTTGA AGAAGATATA GGGAAGTTGA AGTCCTGTCT TACTGGCTTC 
    CTTCAGGAAT ATGGGCTGTC TGTAATGGTG AAAGACGATT ATGTCCATGA ATTTTGCCGG 
    TACGGAGCTG CTGAGCCACA CACCATTGCT GCGTTCCTGG GAGGAGCTGC TGCTCAAGAA 
    GTTATCAAAA TAATCACCAA ACAATTCGTA ATTTTTAATA ATACTTATAT TTATAGTGGT 
    ATGTCACAAA CTTCTGCAAC TTTCCAGTTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006530947.1
    CDS92..1624
    Translation

    Target ORF information:

    RefSeq Version XM_006530884.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus NEDD8 activating enzyme E1 subunit 1 (Nae1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_006530884.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGCGCAGC CAGGGAAGAT ACTCAAGGAG CAAAAGTACG ACCGGCAGCT GAGGCTGTGG 
    GGTGATCATG GACAAGAAGC TTTGGAATCT GCTCATGTTT GCTTAATAAA TGCAACGGCT 
    ACAGGAACTG AAATTCTTAA AAACTTGGTG CTGCCAGGTA TTGGATCATT TACAATTATT 
    GATGGAAATC TGGTCAGCGG AGAAGATGCT GGAAACAACT TTTTCCTTCA AAAAAGCAGT 
    ATTGGCAAGA GTCCAGAAAA CCTTCTAGAC AATGATCCTT CATTTTTCTG TAGGTTTACT 
    ATTGTGGTTG CAACTCAGCT TCTTGAAAGT ACATTGCTGC GTTTAGCAGA TGTTCTCTGG 
    AATTCCCAGA TCCCTCTTTT GATCTGTAGG ACATATGGAC TAGTTGGTTA TATGAGGATT 
    ATTATAAAAG AACACCCAGT AATAGAATCT CATCCAGATA ATGCATTGGA GGATCTACGA 
    CTAGATAAGC CATTTCCTGA ACTGAGAGAA CATCTTCAGT CTTATGACTT GGACCATATG 
    GAAAAAAAGG ACCATAGCCA TACTCCATGG ATTGTGATCA TAGCTAAATA TTTAGCACAG 
    TGGTATAATG AAACAAATGG ACGGATTCCT AAAAGTTATA AAGAAAAAGA GGACTTCAGA 
    GATTTGATAA GACAAGGGAT TTTAAAGAAT GAAAATGGAG CTCCAGAAGA CGAAGAGAAT 
    TTTGAAGAAG CCATCAAAAA TGTGAACACA GCACTAAATA CAACTCAGAT CCCAAGCAGT 
    ATTGAAGATA TATTTAATGA TGATCGATGC ATAAATATCA CCAAACAGAC ACCAACTTTT 
    TGGATTCTAG CTCGTGCCTT AAAGGAATTT GTGGCCAAGG AGGGTCAAGG AAATTTACCT 
    GTCCGAGGCA CAATTCCTGA TATGATTGCA GATTCAAACA AATACATAAA ACTGCAGAAT 
    GTTTACCGAG AGAAAGCAAA GAAAGATGCT GCTGCTGTAG GCAATCACGT TGCCAAATTG 
    CTTCAGTCTG TTGGCCAGGC ACCAGAGTCC ATTTCAGAGA AAGAATTAAA ATTGCTTTGC 
    AGCAATTCTG CATTTCTTCG AGTGGTAAGA TGTCGATCTT TAGCTGAAGA GTATGGCTTG 
    GATACAGTCA ACAAAGATGA AATTATTTCT AGCATGGACA ATCCGGATAA TGAGATAGTC 
    CTGTACCTGA TGTTGCGGGC TGTTGATCGA TTTCATAAAC AGCATGGCAG ATACCCAGGA 
    GTATCTAACT ATCAAGTTGA AGAAGATATA GGGAAGTTGA AGTCCTGTCT TACTGGCTTC 
    CTTCAGGAAT ATGGGCTGTC TGTAATGGTG AAAGACGATT ATGTCCATGA ATTTTGCCGG 
    TACGGAGCTG CTGAGCCACA CACCATTGCT GCGTTCCTGG GAGGAGCTGC TGCTCAAGAA 
    GTTATCAAAA TAATCACCAA ACAATTCGTA ATTTTTAATA ATACTTATAT TTATAGTGGT 
    ATGTCACAAA CTTCTGCAAC TTTCCAGTTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Nae1 ( NM_144931.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_659180.1 Mus musculus NEDD8 activating enzyme E1 subunit 1 (Nae1), mRNA.
    Price & Availability↑
    CloneID OMu15888
    Clone ID Related Accession (Same CDS sequence) NM_144931.3
    Accession Version NM_144931.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-18
    Organism Mus musculus(house mouse)
    Product NEDD8-activating enzyme E1 regulatory subunit
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC130543.3. On Aug 22, 2009 this sequence version replaced NM_144931.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC023680.1, AK146202.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGCGCAGC CAGGGAAGAT ACTCAAGGAG CAAAAGTACG ACCGGCAGCT GAGGCTGTGG 
    GGTGATCATG GACAAGAAGC TTTGGAATCT GCTCATGTTT GCTTAATAAA TGCAACGGCT 
    ACAGGAACTG AAATTCTTAA AAACTTGGTG CTGCCAGGTA TTGGATCATT TACAATTATT 
    GATGGAAATC TGGTCAGCGG AGAAGATGCT GGAAACAACT TTTTCCTTCA AAAAAGCAGT 
    ATTGGCAAGA ACCGAGCTCA AGCTGCCATG GAATTCTTAC AAGAACTAAA TAGCGATGTC 
    TCTGGAAGTT TTGTGGAAGA GAGTCCAGAA AACCTTCTAG ACAATGATCC TTCATTTTTC 
    TGTAGGTTTA CTATTGTGGT TGCAACTCAG CTTCTTGAAA GTACATTGCT GCGTTTAGCA 
    GATGTTCTCT GGAATTCCCA GATCCCTCTT TTGATCTGTA GGACATATGG ACTAGTTGGT 
    TATATGAGGA TTATTATAAA AGAACACCCA GTAATAGAAT CTCATCCAGA TAATGCATTG 
    GAGGATCTAC GACTAGATAA GCCATTTCCT GAACTGAGAG AACATCTTCA GTCTTATGAC 
    TTGGACCATA TGGAAAAAAA GGACCATAGC CATACTCCAT GGATTGTGAT CATAGCTAAA 
    TATTTAGCAC AGTGGTATAA TGAAACAAAT GGACGGATTC CTAAAAGTTA TAAAGAAAAA 
    GAGGACTTCA GAGATTTGAT AAGACAAGGG ATTTTAAAGA ATGAAAATGG AGCTCCAGAA 
    GACGAAGAGA ATTTTGAAGA AGCCATCAAA AATGTGAACA CAGCACTAAA TACAACTCAG 
    ATCCCAAGCA GTATTGAAGA TATATTTAAT GATGATCGAT GCATAAATAT CACCAAACAG 
    ACACCAACTT TTTGGATTCT AGCTCGTGCC TTAAAGGAAT TTGTGGCCAA GGAGGGTCAA 
    GGAAATTTAC CTGTCCGAGG CACAATTCCT GATATGATTG CAGATTCAAA CAAATACATA 
    AAACTGCAGA ATGTTTACCG AGAGAAAGCA AAGAAAGATG CTGCTGCTGT AGGCAATCAC 
    GTTGCCAAAT TGCTTCAGTC TGTTGGCCAG GCACCAGAGT CCATTTCAGA GAAAGAATTA 
    AAATTGCTTT GCAGCAATTC TGCATTTCTT CGAGTGGTAA GATGTCGATC TTTAGCTGAA 
    GAGTATGGCT TGGATACAGT CAACAAAGAT GAAATTATTT CTAGCATGGA CAATCCGGAT 
    AATGAGATAG TCCTGTACCT GATGTTGCGG GCTGTTGATC GATTTCATAA ACAGCATGGC 
    AGATACCCAG GAGTATCTAA CTATCAAGTT GAAGAAGATA TAGGGAAGTT GAAGTCCTGT 
    CTTACTGGCT TCCTTCAGGA ATATGGGCTG TCTGTAATGG TGAAAGACGA TTATGTCCAT 
    GAATTTTGCC GGTACGGAGC TGCTGAGCCA CACACCATTG CTGCGTTCCT GGGAGGAGCT 
    GCTGCTCAAG AAGTTATCAA AATAATCACC AAACAATTCG TAATTTTTAA TAATACTTAT 
    ATTTATAGTG GTATGTCACA AACTTCTGCA ACTTTCCAGT TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_659180.1
    CDS74..1678
    Translation

    Target ORF information:

    RefSeq Version NM_144931.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus NEDD8 activating enzyme E1 subunit 1 (Nae1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_144931.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGCGCAGC CAGGGAAGAT ACTCAAGGAG CAAAAGTACG ACCGGCAGCT GAGGCTGTGG 
    GGTGATCATG GACAAGAAGC TTTGGAATCT GCTCATGTTT GCTTAATAAA TGCAACGGCT 
    ACAGGAACTG AAATTCTTAA AAACTTGGTG CTGCCAGGTA TTGGATCATT TACAATTATT 
    GATGGAAATC TGGTCAGCGG AGAAGATGCT GGAAACAACT TTTTCCTTCA AAAAAGCAGT 
    ATTGGCAAGA ACCGAGCTCA AGCTGCCATG GAATTCTTAC AAGAACTAAA TAGCGATGTC 
    TCTGGAAGTT TTGTGGAAGA GAGTCCAGAA AACCTTCTAG ACAATGATCC TTCATTTTTC 
    TGTAGGTTTA CTATTGTGGT TGCAACTCAG CTTCTTGAAA GTACATTGCT GCGTTTAGCA 
    GATGTTCTCT GGAATTCCCA GATCCCTCTT TTGATCTGTA GGACATATGG ACTAGTTGGT 
    TATATGAGGA TTATTATAAA AGAACACCCA GTAATAGAAT CTCATCCAGA TAATGCATTG 
    GAGGATCTAC GACTAGATAA GCCATTTCCT GAACTGAGAG AACATCTTCA GTCTTATGAC 
    TTGGACCATA TGGAAAAAAA GGACCATAGC CATACTCCAT GGATTGTGAT CATAGCTAAA 
    TATTTAGCAC AGTGGTATAA TGAAACAAAT GGACGGATTC CTAAAAGTTA TAAAGAAAAA 
    GAGGACTTCA GAGATTTGAT AAGACAAGGG ATTTTAAAGA ATGAAAATGG AGCTCCAGAA 
    GACGAAGAGA ATTTTGAAGA AGCCATCAAA AATGTGAACA CAGCACTAAA TACAACTCAG 
    ATCCCAAGCA GTATTGAAGA TATATTTAAT GATGATCGAT GCATAAATAT CACCAAACAG 
    ACACCAACTT TTTGGATTCT AGCTCGTGCC TTAAAGGAAT TTGTGGCCAA GGAGGGTCAA 
    GGAAATTTAC CTGTCCGAGG CACAATTCCT GATATGATTG CAGATTCAAA CAAATACATA 
    AAACTGCAGA ATGTTTACCG AGAGAAAGCA AAGAAAGATG CTGCTGCTGT AGGCAATCAC 
    GTTGCCAAAT TGCTTCAGTC TGTTGGCCAG GCACCAGAGT CCATTTCAGA GAAAGAATTA 
    AAATTGCTTT GCAGCAATTC TGCATTTCTT CGAGTGGTAA GATGTCGATC TTTAGCTGAA 
    GAGTATGGCT TGGATACAGT CAACAAAGAT GAAATTATTT CTAGCATGGA CAATCCGGAT 
    AATGAGATAG TCCTGTACCT GATGTTGCGG GCTGTTGATC GATTTCATAA ACAGCATGGC 
    AGATACCCAG GAGTATCTAA CTATCAAGTT GAAGAAGATA TAGGGAAGTT GAAGTCCTGT 
    CTTACTGGCT TCCTTCAGGA ATATGGGCTG TCTGTAATGG TGAAAGACGA TTATGTCCAT 
    GAATTTTGCC GGTACGGAGC TGCTGAGCCA CACACCATTG CTGCGTTCCT GGGAGGAGCT 
    GCTGCTCAAG AAGTTATCAA AATAATCACC AAACAATTCG TAATTTTTAA TAATACTTAT 
    ATTTATAGTG GTATGTCACA AACTTCTGCA ACTTTCCAGT TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Nae1 ( XM_006530884.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530947.1 Mus musculus NEDD8 activating enzyme E1 subunit 1 (Nae1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OMu41369
    Clone ID Related Accession (Same CDS sequence) XM_006530884.3 , XM_006530884.2
    Accession Version XM_006530884.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1533bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NEDD8-activating enzyme E1 regulatory subunit isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530884.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGCGCAGC CAGGGAAGAT ACTCAAGGAG CAAAAGTACG ACCGGCAGCT GAGGCTGTGG 
    GGTGATCATG GACAAGAAGC TTTGGAATCT GCTCATGTTT GCTTAATAAA TGCAACGGCT 
    ACAGGAACTG AAATTCTTAA AAACTTGGTG CTGCCAGGTA TTGGATCATT TACAATTATT 
    GATGGAAATC TGGTCAGCGG AGAAGATGCT GGAAACAACT TTTTCCTTCA AAAAAGCAGT 
    ATTGGCAAGA GTCCAGAAAA CCTTCTAGAC AATGATCCTT CATTTTTCTG TAGGTTTACT 
    ATTGTGGTTG CAACTCAGCT TCTTGAAAGT ACATTGCTGC GTTTAGCAGA TGTTCTCTGG 
    AATTCCCAGA TCCCTCTTTT GATCTGTAGG ACATATGGAC TAGTTGGTTA TATGAGGATT 
    ATTATAAAAG AACACCCAGT AATAGAATCT CATCCAGATA ATGCATTGGA GGATCTACGA 
    CTAGATAAGC CATTTCCTGA ACTGAGAGAA CATCTTCAGT CTTATGACTT GGACCATATG 
    GAAAAAAAGG ACCATAGCCA TACTCCATGG ATTGTGATCA TAGCTAAATA TTTAGCACAG 
    TGGTATAATG AAACAAATGG ACGGATTCCT AAAAGTTATA AAGAAAAAGA GGACTTCAGA 
    GATTTGATAA GACAAGGGAT TTTAAAGAAT GAAAATGGAG CTCCAGAAGA CGAAGAGAAT 
    TTTGAAGAAG CCATCAAAAA TGTGAACACA GCACTAAATA CAACTCAGAT CCCAAGCAGT 
    ATTGAAGATA TATTTAATGA TGATCGATGC ATAAATATCA CCAAACAGAC ACCAACTTTT 
    TGGATTCTAG CTCGTGCCTT AAAGGAATTT GTGGCCAAGG AGGGTCAAGG AAATTTACCT 
    GTCCGAGGCA CAATTCCTGA TATGATTGCA GATTCAAACA AATACATAAA ACTGCAGAAT 
    GTTTACCGAG AGAAAGCAAA GAAAGATGCT GCTGCTGTAG GCAATCACGT TGCCAAATTG 
    CTTCAGTCTG TTGGCCAGGC ACCAGAGTCC ATTTCAGAGA AAGAATTAAA ATTGCTTTGC 
    AGCAATTCTG CATTTCTTCG AGTGGTAAGA TGTCGATCTT TAGCTGAAGA GTATGGCTTG 
    GATACAGTCA ACAAAGATGA AATTATTTCT AGCATGGACA ATCCGGATAA TGAGATAGTC 
    CTGTACCTGA TGTTGCGGGC TGTTGATCGA TTTCATAAAC AGCATGGCAG ATACCCAGGA 
    GTATCTAACT ATCAAGTTGA AGAAGATATA GGGAAGTTGA AGTCCTGTCT TACTGGCTTC 
    CTTCAGGAAT ATGGGCTGTC TGTAATGGTG AAAGACGATT ATGTCCATGA ATTTTGCCGG 
    TACGGAGCTG CTGAGCCACA CACCATTGCT GCGTTCCTGG GAGGAGCTGC TGCTCAAGAA 
    GTTATCAAAA TAATCACCAA ACAATTCGTA ATTTTTAATA ATACTTATAT TTATAGTGGT 
    ATGTCACAAA CTTCTGCAAC TTTCCAGTTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006530947.1
    CDS93..1625
    Translation

    Target ORF information:

    RefSeq Version XM_006530884.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus NEDD8 activating enzyme E1 subunit 1 (Nae1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_006530884.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGCGCAGC CAGGGAAGAT ACTCAAGGAG CAAAAGTACG ACCGGCAGCT GAGGCTGTGG 
    GGTGATCATG GACAAGAAGC TTTGGAATCT GCTCATGTTT GCTTAATAAA TGCAACGGCT 
    ACAGGAACTG AAATTCTTAA AAACTTGGTG CTGCCAGGTA TTGGATCATT TACAATTATT 
    GATGGAAATC TGGTCAGCGG AGAAGATGCT GGAAACAACT TTTTCCTTCA AAAAAGCAGT 
    ATTGGCAAGA GTCCAGAAAA CCTTCTAGAC AATGATCCTT CATTTTTCTG TAGGTTTACT 
    ATTGTGGTTG CAACTCAGCT TCTTGAAAGT ACATTGCTGC GTTTAGCAGA TGTTCTCTGG 
    AATTCCCAGA TCCCTCTTTT GATCTGTAGG ACATATGGAC TAGTTGGTTA TATGAGGATT 
    ATTATAAAAG AACACCCAGT AATAGAATCT CATCCAGATA ATGCATTGGA GGATCTACGA 
    CTAGATAAGC CATTTCCTGA ACTGAGAGAA CATCTTCAGT CTTATGACTT GGACCATATG 
    GAAAAAAAGG ACCATAGCCA TACTCCATGG ATTGTGATCA TAGCTAAATA TTTAGCACAG 
    TGGTATAATG AAACAAATGG ACGGATTCCT AAAAGTTATA AAGAAAAAGA GGACTTCAGA 
    GATTTGATAA GACAAGGGAT TTTAAAGAAT GAAAATGGAG CTCCAGAAGA CGAAGAGAAT 
    TTTGAAGAAG CCATCAAAAA TGTGAACACA GCACTAAATA CAACTCAGAT CCCAAGCAGT 
    ATTGAAGATA TATTTAATGA TGATCGATGC ATAAATATCA CCAAACAGAC ACCAACTTTT 
    TGGATTCTAG CTCGTGCCTT AAAGGAATTT GTGGCCAAGG AGGGTCAAGG AAATTTACCT 
    GTCCGAGGCA CAATTCCTGA TATGATTGCA GATTCAAACA AATACATAAA ACTGCAGAAT 
    GTTTACCGAG AGAAAGCAAA GAAAGATGCT GCTGCTGTAG GCAATCACGT TGCCAAATTG 
    CTTCAGTCTG TTGGCCAGGC ACCAGAGTCC ATTTCAGAGA AAGAATTAAA ATTGCTTTGC 
    AGCAATTCTG CATTTCTTCG AGTGGTAAGA TGTCGATCTT TAGCTGAAGA GTATGGCTTG 
    GATACAGTCA ACAAAGATGA AATTATTTCT AGCATGGACA ATCCGGATAA TGAGATAGTC 
    CTGTACCTGA TGTTGCGGGC TGTTGATCGA TTTCATAAAC AGCATGGCAG ATACCCAGGA 
    GTATCTAACT ATCAAGTTGA AGAAGATATA GGGAAGTTGA AGTCCTGTCT TACTGGCTTC 
    CTTCAGGAAT ATGGGCTGTC TGTAATGGTG AAAGACGATT ATGTCCATGA ATTTTGCCGG 
    TACGGAGCTG CTGAGCCACA CACCATTGCT GCGTTCCTGG GAGGAGCTGC TGCTCAAGAA 
    GTTATCAAAA TAATCACCAA ACAATTCGTA ATTTTTAATA ATACTTATAT TTATAGTGGT 
    ATGTCACAAA CTTCTGCAAC TTTCCAGTTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    High-throughput discovery of novel developmental phenotypes.
    Nature537(7621)508-514(2016 09)
    Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA


    Neddylation inhibition impairs spine development, destabilizes synapses and deteriorates cognition.
    Nature neuroscience18(2)239-51(2015 Feb)
    Vogl AM,Brockmann MM,Giusti SA,Maccarrone G,Vercelli CA,Bauder CA,Richter JS,Roselli F,Hafner AS,Dedic N,Wotjak CT,Vogt-Weisenhorn DM,Choquet D,Turck CW,Stein V,Deussing JM,Refojo D


    Amyloid precursor protein binding protein-1 modulates cell cycle progression in fetal neural stem cells.
    PloS one5(12)e14203(2010 Dec)
    Joo Y,Ha S,Hong BH,Kim Ja,Chang KA,Liew H,Kim S,Sun W,Kim JH,Chong YH,Suh YH,Kim HS


    The mechanism of poly-NEDD8 chain formation in vitro.
    Biochemical and biophysical research communications381(3)443-7(2009 Apr)
    Ohki Y,Funatsu N,Konishi N,Chiba T


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