Phf20 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Phf20
Entrez Gene ID	228829
Full Name	PHD finger protein 20
Synonyms	6820402O20Rik
General protein information	Preferred Names PHD finger protein 20 Names PHD finger protein 20 hepatocellular carcinoma-associated antigen 58 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 H1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006499339.1	XP_006499402.1	PHD finger protein 20 isoform X3
XM_006499338.3	XP_006499401.1	PHD finger protein 20 isoform X2
NM_172674.2	NP_766262.2	PHD finger protein 20
XM_006499339.2	XP_006499402.1	PHD finger protein 20 isoform X3
XM_006499338.4	XP_006499401.1	PHD finger protein 20 isoform X2
XM_017317827.1	XP_017173316.1	PHD finger protein 20 isoform X1
XM_017317827.2	XP_017173316.1	PHD finger protein 20 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Phf20	NP_001101265.1
Gallus gallus (chicken)	PHF20	XP_004946939.1
	PHF20	XP_002798212.1
Bos taurus (cattle)	PHF20	NP_001161898.1
Mus musculus (house mouse)	Phf20	NP_766262.2
Homo sapiens (human)	PHF20	NP_057520.2
Pan troglodytes (chimpanzee)	PHF20	XP_001165874.2
Canis lupus familiaris (dog)	PHF20	XP_852250.1
Xenopus tropicalis (tropical clawed frog)	phf20	XP_002932906.1

Gene Ontology
Bibliography

Related articles in PubMed

An efficient method for generation of bi-allelic null mutant mouse embryonic stem cells and its application for investigating epigenetic modifiers.
Fisher CL, Marks H, Cho LT, Andrews R, Wormald S, Carroll T, Iyer V, Tate P, Rosen B, Stunnenberg HG, Fisher AG, Skarnes WC
Nucleic acids research45(21)e174(2017 Dec)

PHF20 positively regulates osteoblast differentiation via increasing the expression and activation of Runx2 with enrichment of H3K4me3.
Yang JW, Jeong BC, Park J, Koh JT
Scientific reports7(1)8060(2017 Aug)

Jmjd3 inhibits reprogramming by upregulating expression of INK4a/Arf and targeting PHF20 for ubiquitination.
Zhao W, Li Q, Ayers S, Gu Y, Shi Z, Zhu Q, Chen Y, Wang HY, Wang RF
Cell152(5)1037-50(2013 Feb)

Loss of the methyl lysine effector protein PHF20 impacts the expression of genes regulated by the lysine acetyltransferase MOF.
Badeaux AI, Yang Y, Cardenas K, Vemulapalli V, Chen K, Kusewitt D, Richie E, Li W, Bedford MT
The Journal of biological chemistry287(1)429-37(2012 Jan)

Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst.
Guo G, Huss M, Tong GQ, Wang C, Li Sun L, Clarke ND, Robson P
Developmental cell18(4)675-85(2010 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PHF20 is not required for maintaining the global H4K16 acetylation levels or locus specific histone acetylation but instead works downstream in transcriptional regulation of MOF target genes
Title: Loss of the methyl lysine effector protein PHF20 impacts the expression of genes regulated by the lysine acetyltransferase MOF.

The following Phf20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Phf20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu30368	XM_006499338.4 Latest version!	Mus musculus PHD finger protein 20 (Phf20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu30369	XM_006499339.2 Latest version!	Mus musculus PHD finger protein 20 (Phf20), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu80233	XM_017317827.2 Latest version!	Mus musculus PHD finger protein 20 (Phf20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu20847	NM_172674.2 Latest version!	Mus musculus PHD finger protein 20 (Phf20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$594.30 $849.00
View Old Accession Versions >>
OMu30369	XM_006499339.1	Mus musculus PHD finger protein 20 (Phf20), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu30368	XM_006499338.3	Mus musculus PHD finger protein 20 (Phf20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu80233	XM_017317827.1	Mus musculus PHD finger protein 20 (Phf20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Phf20 ( XM_006499339.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499402.1 Mus musculus PHD finger protein 20 (Phf20), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu30369
Clone ID Related Accession (Same CDS sequence)	XM_006499339.1 , XM_006499339.2
Accession Version	XM_006499339.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	PHD finger protein 20 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAAA ACACGATGAA AACAGAACCT GTTTCTCCTC TTGCTGAGGT GCAGGAAGTT 
TCAACTGTTG AAGTTCCAAA TACTTTGAAG AAAGTTGATG ACTCTGTGAC GTTGAATGTG 
CCAGCTGTGG ACCTAGACCA CAAGTTTCGA TGCAAGGTTC TGGACTGTTT GAAATTTTTC 
CGCAAGGCTA AATTGCTGCA CTATCATATG AAGTATTTCC ATGGGATGGA GAAGTCACCA 
GAGCCAGAGG AGGGCCCAGG GAAGACGCAT GTACAGACTC GGGGCTCTGC GGTGCCTGAC 
AAGACAAGCC AGGAGAGCCT AACCAGGAAG CGGGTCTCTG CCAGTTCCCC CACTGCAAAA 
GAGAAGGAAA AGACTAAAGA GAAGAAATTC AAAGAACTTG TGAGAGTGAA GCCAAAGAAG 
AAAAAGAAAA AGAAAAAGAA AACCAAGCCT GAATGTCCCT GCAGTGAGGA CATCAGTGAT 
ACTTCCCAGG AACCTTCTCC ACCCAAAACA TTTGCTGTCA CCAGGTGTGG GTCCTCACAC 
AAGCCTGGGG TCCATATGAG CCCGCAGCTC CATGGTTCAG ATAATGGAAA CCACAAAGGG 
AAATTGAAAA CCTGTGAGGA GGATAATTTG AGTGAGTCCT CTTCCGAGAG CTTTCTTTGG 
AGTGATGAGG AATATGGTCA AGATGTTGAT GTGACCACCA ACCCAGATGA GGAGCTTGAG 
GGCGACGACC GCTATGATTT TGAGGTGGTC CGCTGCATCT GTGAAGTGCA GGAGGAAAAT 
GACTTCATGA TTCAGTGTGA AGAGTGCCAG TGCTGGCAGC ACGGGGTCTG CATGGGCTTA 
CTGGAAGAAA ACGTGCCTGA GAAATACACC TGCTATGTTT GCCAAGACCC TCCAGGTCAG 
AGGCCTGGCT TCAAGTACTG GTATGACAAG GAGTGGCTGA GCCGGGGACA CATGCATGGT 
CTGGCATTTC TGGATCAGAA CTACTCCCAC CAGAATGCCA GGAAGATCGT GGCCACCCAC 
CAGCTGCTCG GGGACGTGCA GAGAGTGATC CAAGTGCTGC ACGGCCTGCA GCTCAAGATG 
AGCATTCTGC AAAGCAGAGA GCATCCTGAT CTGCAGCTAT GGTGCCAGCC CTGGAAACAG 
CACTCGGGGG AGGGAAGAGC ACATCCCAGA CACATCCACA TCACAGACGC CAGGAGCGAG 
GAGTCCCCAA GCTATAGAAC TTTGAATGGG GCGGTGGAGA AGCCATCGCC CCTGCCCCGA 
TCTGTGGAGG AGTCTTACAT CACCAGTGAG CATTGCTACC AGAAACCTCG CGCCTATTAT 
CCCGCTGTGG AGCAGAGGCT GGTCGTGGAG ACGAGAGGCT CTGCCCTTGA TGCTGCAGTC 
AGCCCCCTCT GTGAGAACGG CGACGACTCC CTCTCCCCTC GCCTAGGCTG GCCCATCGAC 
CAAGACAGGA GCAGAGGCGA CATAGATCCC AAACCCAGCT CCCCAAAGGT GAGAGAGTAC 
ATTTCCAAGA ATGTCTTGCC AGAAGAGACG CCTGCAAGGA AGCTGCTGGA CAGAGGTGGA 
GAAGGGCTGG TGAGCTCTCA GCACCAGTGG CAGTTCAACC TGCTCACACA TGTGGAGTCC 
CTGCAGGATG AGGTGACGCA CCGGATGGAC TCCATTGAGA AGGAGCTGGA CGTGCTGGAG 
AGCTGGCTGG ACTACACTGG GGAGCTGGAG CCCCCAGAGC CACTGGCCAG GCTTCCGCAG 
CTCAAGCACT GCATCAAGCA GCTGCTGACT GACCTGGGCA AGGTGCAGCA GATCGCCCTC 
TGCTGCTCGA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499402.1
CDS	1203..3017
Translation	MGENTMKTEPVSPLAEVQEVSTVEVPNTLKKVDDSVTLNVPAVDLDHKFRCKVLDCLKFFRKAKLLHYHMKYFHGMEKSPEPEEGPGKTHVQTRGSAVPDKTSQESLTRKRVSASSPTAKEKEKTKEKKFKELVRVKPKKKKKKKKKTKPECPCSEDISDTSQEPSPPKTFAVTRCGSSHKPGVHMSPQLHGSDNGNHKGKLKTCEEDNLSESSSESFLWSDEEYGQDVDVTTNPDEELEGDDRYDFEVVRCICEVQEENDFMIQCEECQCWQHGVCMGLLEENVPEKYTCYVCQDPPGQRPGFKYWYDKEWLSRGHMHGLAFLDQNYSHQNARKIVATHQLLGDVQRVIQVLHGLQLKMSILQSREHPDLQLWCQPWKQHSGEGRAHPRHIHITDARSEESPSYRTLNGAVEKPSPLPRSVEESYITSEHCYQKPRAYYPAVEQRLVVETRGSALDAAVSPLCENGDDSLSPRLGWPIDQDRSRGDIDPKPSSPKVREYISKNVLPEETPARKLLDRGGEGLVSSQHQWQFNLLTHVESLQDEVTHRMDSIEKELDVLESWLDYTGELEPPEPLARLPQLKHCIKQLLTDLGKVQQIALCCST

Target ORF information:

RefSeq Version	XM_006499339.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus PHD finger protein 20 (Phf20), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499339.1

ORF Insert Sequence:

ATGGGAGAAA ACACGATGAA AACAGAACCT GTTTCTCCTC TTGCTGAGGT GCAGGAAGTT 
TCAACTGTTG AAGTTCCAAA TACTTTGAAG AAAGTTGATG ACTCTGTGAC GTTGAATGTG 
CCAGCTGTGG ACCTAGACCA CAAGTTTCGA TGCAAGGTTC TGGACTGTTT GAAATTTTTC 
CGCAAGGCTA AATTGCTGCA CTATCATATG AAGTATTTCC ATGGGATGGA GAAGTCACCA 
GAGCCAGAGG AGGGCCCAGG GAAGACGCAT GTACAGACTC GGGGCTCTGC GGTGCCTGAC 
AAGACAAGCC AGGAGAGCCT AACCAGGAAG CGGGTCTCTG CCAGTTCCCC CACTGCAAAA 
GAGAAGGAAA AGACTAAAGA GAAGAAATTC AAAGAACTTG TGAGAGTGAA GCCAAAGAAG 
AAAAAGAAAA AGAAAAAGAA AACCAAGCCT GAATGTCCCT GCAGTGAGGA CATCAGTGAT 
ACTTCCCAGG AACCTTCTCC ACCCAAAACA TTTGCTGTCA CCAGGTGTGG GTCCTCACAC 
AAGCCTGGGG TCCATATGAG CCCGCAGCTC CATGGTTCAG ATAATGGAAA CCACAAAGGG 
AAATTGAAAA CCTGTGAGGA GGATAATTTG AGTGAGTCCT CTTCCGAGAG CTTTCTTTGG 
AGTGATGAGG AATATGGTCA AGATGTTGAT GTGACCACCA ACCCAGATGA GGAGCTTGAG 
GGCGACGACC GCTATGATTT TGAGGTGGTC CGCTGCATCT GTGAAGTGCA GGAGGAAAAT 
GACTTCATGA TTCAGTGTGA AGAGTGCCAG TGCTGGCAGC ACGGGGTCTG CATGGGCTTA 
CTGGAAGAAA ACGTGCCTGA GAAATACACC TGCTATGTTT GCCAAGACCC TCCAGGTCAG 
AGGCCTGGCT TCAAGTACTG GTATGACAAG GAGTGGCTGA GCCGGGGACA CATGCATGGT 
CTGGCATTTC TGGATCAGAA CTACTCCCAC CAGAATGCCA GGAAGATCGT GGCCACCCAC 
CAGCTGCTCG GGGACGTGCA GAGAGTGATC CAAGTGCTGC ACGGCCTGCA GCTCAAGATG 
AGCATTCTGC AAAGCAGAGA GCATCCTGAT CTGCAGCTAT GGTGCCAGCC CTGGAAACAG 
CACTCGGGGG AGGGAAGAGC ACATCCCAGA CACATCCACA TCACAGACGC CAGGAGCGAG 
GAGTCCCCAA GCTATAGAAC TTTGAATGGG GCGGTGGAGA AGCCATCGCC CCTGCCCCGA 
TCTGTGGAGG AGTCTTACAT CACCAGTGAG CATTGCTACC AGAAACCTCG CGCCTATTAT 
CCCGCTGTGG AGCAGAGGCT GGTCGTGGAG ACGAGAGGCT CTGCCCTTGA TGCTGCAGTC 
AGCCCCCTCT GTGAGAACGG CGACGACTCC CTCTCCCCTC GCCTAGGCTG GCCCATCGAC 
CAAGACAGGA GCAGAGGCGA CATAGATCCC AAACCCAGCT CCCCAAAGGT GAGAGAGTAC 
ATTTCCAAGA ATGTCTTGCC AGAAGAGACG CCTGCAAGGA AGCTGCTGGA CAGAGGTGGA 
GAAGGGCTGG TGAGCTCTCA GCACCAGTGG CAGTTCAACC TGCTCACACA TGTGGAGTCC 
CTGCAGGATG AGGTGACGCA CCGGATGGAC TCCATTGAGA AGGAGCTGGA CGTGCTGGAG 
AGCTGGCTGG ACTACACTGG GGAGCTGGAG CCCCCAGAGC CACTGGCCAG GCTTCCGCAG 
CTCAAGCACT GCATCAAGCA GCTGCTGACT GACCTGGGCA AGGTGCAGCA GATCGCCCTC 
TGCTGCTCGA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phf20 ( XM_006499338.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499401.1 Mus musculus PHD finger protein 20 (Phf20), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu30368
Clone ID Related Accession (Same CDS sequence)	XM_006499338.3 , XM_006499338.4
Accession Version	XM_006499338.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	PHD finger protein 20 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499338.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAAGC ACCCACCTAA CAGACGAGGA ATCAGCTTTG AAGTGGGAGC CCAGTTGGAA 
GCTCGGGACC GTTTAAAAAA CTGGTATCCA GCTCATATTG AAGACATTGA CTACGAAGAA 
GGGAGAGTTC TCATCCATTT CAAGCGTTGG AACCATCGTT ACGATGAGTG GTTTTGCTGG 
GACAGTCCTT ATCTGCGCCC TTTAGAGAAG ATCCAGCTGA GGAAAGAGGG TTTACATGAT 
GAGGATGGCT CTTCTGAATT TCAAATAAAC CAGCAAGTGC TTGCTTGCTG GTCTGACTGT 
CGATTTTATC CAGCCAGAGT CACTGCTGTG AACAAGGATG CTGTGGATTC AAACTCTCAA 
ACTTTGCAAC CAATAACATT GGAATTGAGA AGACGGAAAA TATCAAAACG AAGTGACACC 
CCATTAAAGC GTCCCAGACT CGACAAAAAT TCACCCCAGG AACAGTCAAA AAAACGCTCT 
GAAAATAGTG ACAAAGACTT ATCCAGGAGA CGGTCCTCCA GGCTGTCCAC TAATGGGACC 
CGTGAGATCC TAGATCCTGA CTCGATTGTA CCTGATCTGG TTCATACGGT TGATACAAAC 
CCTCTACCAG ACAAGTCACC CAGTGCCAAG GATTCTGCTG AAGGTCAGTT GAAGTCTCCA 
TTGGAAGCTG GCCAGGTCTC TTCTGCATTA ACTTGCCACC CCATTGGGGA TGGCCTGGGG 
GCAGCAGATT TGGAGTTGAA TTGCAAGTCA ATGGGAGAAA ACACGATGAA AACAGAACCT 
GTTTCTCCTC TTGCTGAGGT GCAGGAAGTT TCAACTGTTG AAGTTCCAAA TACTTTGAAG 
AAAGTTGATG ACTCTGTGAC GTTGAATGTG CCAGCTGTGG ACCTAGACCA CAAGTTTCGA 
TGCAAGGTTC TGGACTGTTT GAAATTTTTC CGCAAGGCTA AATTGCTGCA CTATCATATG 
AAGTATTTCC ATGGGATGGA GAAGTCACCA GAGCCAGAGG AGGGCCCAGG GAAGACGCAT 
GTACAGACTC GGGGCTCTGC GGTGCCTGAC AAGACAAGCC AGGAGAGCCT AACCAGGAAG 
CGGGTCTCTG CCAGTTCCCC CACTGCAAAA GAGAAGGAAA AGACTAAAGA GAAGAAATTC 
AAAGAACTTG TGAGAGTGAA GCCAAAGAAG AAAAAGAAAA AGAAAAAGAA AACCAAGCCT 
GAATGTCCCT GCAGTGAGGA CATCAGTGAT ACTTCCCAGG AACCTTCTCC ACCCAAAACA 
TTTGCTGTCA CCAGGTGTGG GTCCTCACAC AAGCCTGGGG TCCATATGAG CCCGCAGCTC 
CATGGTTCAG ATAATGGAAA CCACAAAGGG AAATTGAAAA CCTGTGAGGA GGATAATTTG 
AGTGAGTCCT CTTCCGAGAG CTTTCTTTGG AGTGATGAGG AATATGGTCA AGATGTTGAT 
GTGACCACCA ACCCAGATGA GGAGCTTGAG GGCGACGACC GCTATGATTT TGAGGTGGTC 
CGCTGCATCT GTGAAGTGCA GGAGGAAAAT GACTTCATGA TTCAGTGTGA AGAGTGCCAG 
TGCTGGCAGC ACGGGGTCTG CATGGGCTTA CTGGAAGAAA ACGTGCCTGA GAAATACACC 
TGCTATGTTT GCCAAGACCC TCCAGGTCAG AGGCCTGGCT TCAAGTACTG GTATGACAAG 
GAGTGGCTGA GCCGGGGACA CATGCATGGT CTGGCATTTC TGGATCAGAA CTACTCCCAC 
CAGAATGCCA GGAAGATCGT GGCCACCCAC CAGCTGCTCG GGGACGTGCA GAGAGTGATC 
CAAGTGCTGC ACGGCCTGCA GCTCAAGATG AGCATTCTGC AAAGCAGAGA GCATCCTGAT 
CTGCAGCTAT GGTGCCAGCC CTGGAAACAG CACTCGGGGG AGGGAAGAGC ACATCCCAGA 
CACATCCACA TCACAGACGC CAGGAGCGAG GAGTCCCCAA GCTATAGAAC TTTGAATGGG 
GCGGTGGAGA AGCCATCGCC CCTGCCCCGA TCTGTGGAGG AGTCTTACAT CACCAGTGAG 
CATTGCTACC AGAAACCTCG CGCCTATTAT CCCGCTGTGG AGCAGAGGCT GGTCGTGGAG 
ACGAGAGGCT CTGCCCTTGA TGCTGCAGTC AGCCCCCTCT GTGAGAACGG CGACGACTCC 
CTCTCCCCTC GCCTAGGCTG GCCCATCGAC CAAGACAGGA GCAGAGGCGA CATAGATCCC 
AAACCCAGCT CCCCAAAGGT GAGAGAGTAC ATTTCCAAGA ATGTCTTGCC AGAAGAGACG 
CCTGCAAGGA AGCTGCTGGA CAGAGGTGGA GAAGGGCTGG TGAGCTCTCA GCACCAGTGG 
CAGTTCAACC TGCTCACACA TGTGGAGTCC CTGCAGGATG AGGTGACGCA CCGGATGGAC 
TCCATTGAGA AGGAGCTGGA CGTGCTGGAG AGCTGGCTGG ACTACACTGG GGAGCTGGAG 
CCCCCAGAGC CACTGGCCAG GCTTCCGCAG CTCAAGCACT GCATCAAGCA GCTGCTGACT 
GACCTGGGCA AGGTGCAGCA GATCGCCCTC TGCTGCTCGA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499401.1
CDS	118..2682
Translation	MTKHPPNRRGISFEVGAQLEARDRLKNWYPAHIEDIDYEEGRVLIHFKRWNHRYDEWFCWDSPYLRPLEKIQLRKEGLHDEDGSSEFQINQQVLACWSDCRFYPARVTAVNKDAVDSNSQTLQPITLELRRRKISKRSDTPLKRPRLDKNSPQEQSKKRSENSDKDLSRRRSSRLSTNGTREILDPDSIVPDLVHTVDTNPLPDKSPSAKDSAEGQLKSPLEAGQVSSALTCHPIGDGLGAADLELNCKSMGENTMKTEPVSPLAEVQEVSTVEVPNTLKKVDDSVTLNVPAVDLDHKFRCKVLDCLKFFRKAKLLHYHMKYFHGMEKSPEPEEGPGKTHVQTRGSAVPDKTSQESLTRKRVSASSPTAKEKEKTKEKKFKELVRVKPKKKKKKKKKTKPECPCSEDISDTSQEPSPPKTFAVTRCGSSHKPGVHMSPQLHGSDNGNHKGKLKTCEEDNLSESSSESFLWSDEEYGQDVDVTTNPDEELEGDDRYDFEVVRCICEVQEENDFMIQCEECQCWQHGVCMGLLEENVPEKYTCYVCQDPPGQRPGFKYWYDKEWLSRGHMHGLAFLDQNYSHQNARKIVATHQLLGDVQRVIQVLHGLQLKMSILQSREHPDLQLWCQPWKQHSGEGRAHPRHIHITDARSEESPSYRTLNGAVEKPSPLPRSVEESYITSEHCYQKPRAYYPAVEQRLVVETRGSALDAAVSPLCENGDDSLSPRLGWPIDQDRSRGDIDPKPSSPKVREYISKNVLPEETPARKLLDRGGEGLVSSQHQWQFNLLTHVESLQDEVTHRMDSIEKELDVLESWLDYTGELEPPEPLARLPQLKHCIKQLLTDLGKVQQIALCCST

Target ORF information:

RefSeq Version	XM_006499338.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus PHD finger protein 20 (Phf20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499338.3

ORF Insert Sequence:

ATGACCAAGC ACCCACCTAA CAGACGAGGA ATCAGCTTTG AAGTGGGAGC CCAGTTGGAA 
GCTCGGGACC GTTTAAAAAA CTGGTATCCA GCTCATATTG AAGACATTGA CTACGAAGAA 
GGGAGAGTTC TCATCCATTT CAAGCGTTGG AACCATCGTT ACGATGAGTG GTTTTGCTGG 
GACAGTCCTT ATCTGCGCCC TTTAGAGAAG ATCCAGCTGA GGAAAGAGGG TTTACATGAT 
GAGGATGGCT CTTCTGAATT TCAAATAAAC CAGCAAGTGC TTGCTTGCTG GTCTGACTGT 
CGATTTTATC CAGCCAGAGT CACTGCTGTG AACAAGGATG CTGTGGATTC AAACTCTCAA 
ACTTTGCAAC CAATAACATT GGAATTGAGA AGACGGAAAA TATCAAAACG AAGTGACACC 
CCATTAAAGC GTCCCAGACT CGACAAAAAT TCACCCCAGG AACAGTCAAA AAAACGCTCT 
GAAAATAGTG ACAAAGACTT ATCCAGGAGA CGGTCCTCCA GGCTGTCCAC TAATGGGACC 
CGTGAGATCC TAGATCCTGA CTCGATTGTA CCTGATCTGG TTCATACGGT TGATACAAAC 
CCTCTACCAG ACAAGTCACC CAGTGCCAAG GATTCTGCTG AAGGTCAGTT GAAGTCTCCA 
TTGGAAGCTG GCCAGGTCTC TTCTGCATTA ACTTGCCACC CCATTGGGGA TGGCCTGGGG 
GCAGCAGATT TGGAGTTGAA TTGCAAGTCA ATGGGAGAAA ACACGATGAA AACAGAACCT 
GTTTCTCCTC TTGCTGAGGT GCAGGAAGTT TCAACTGTTG AAGTTCCAAA TACTTTGAAG 
AAAGTTGATG ACTCTGTGAC GTTGAATGTG CCAGCTGTGG ACCTAGACCA CAAGTTTCGA 
TGCAAGGTTC TGGACTGTTT GAAATTTTTC CGCAAGGCTA AATTGCTGCA CTATCATATG 
AAGTATTTCC ATGGGATGGA GAAGTCACCA GAGCCAGAGG AGGGCCCAGG GAAGACGCAT 
GTACAGACTC GGGGCTCTGC GGTGCCTGAC AAGACAAGCC AGGAGAGCCT AACCAGGAAG 
CGGGTCTCTG CCAGTTCCCC CACTGCAAAA GAGAAGGAAA AGACTAAAGA GAAGAAATTC 
AAAGAACTTG TGAGAGTGAA GCCAAAGAAG AAAAAGAAAA AGAAAAAGAA AACCAAGCCT 
GAATGTCCCT GCAGTGAGGA CATCAGTGAT ACTTCCCAGG AACCTTCTCC ACCCAAAACA 
TTTGCTGTCA CCAGGTGTGG GTCCTCACAC AAGCCTGGGG TCCATATGAG CCCGCAGCTC 
CATGGTTCAG ATAATGGAAA CCACAAAGGG AAATTGAAAA CCTGTGAGGA GGATAATTTG 
AGTGAGTCCT CTTCCGAGAG CTTTCTTTGG AGTGATGAGG AATATGGTCA AGATGTTGAT 
GTGACCACCA ACCCAGATGA GGAGCTTGAG GGCGACGACC GCTATGATTT TGAGGTGGTC 
CGCTGCATCT GTGAAGTGCA GGAGGAAAAT GACTTCATGA TTCAGTGTGA AGAGTGCCAG 
TGCTGGCAGC ACGGGGTCTG CATGGGCTTA CTGGAAGAAA ACGTGCCTGA GAAATACACC 
TGCTATGTTT GCCAAGACCC TCCAGGTCAG AGGCCTGGCT TCAAGTACTG GTATGACAAG 
GAGTGGCTGA GCCGGGGACA CATGCATGGT CTGGCATTTC TGGATCAGAA CTACTCCCAC 
CAGAATGCCA GGAAGATCGT GGCCACCCAC CAGCTGCTCG GGGACGTGCA GAGAGTGATC 
CAAGTGCTGC ACGGCCTGCA GCTCAAGATG AGCATTCTGC AAAGCAGAGA GCATCCTGAT 
CTGCAGCTAT GGTGCCAGCC CTGGAAACAG CACTCGGGGG AGGGAAGAGC ACATCCCAGA 
CACATCCACA TCACAGACGC CAGGAGCGAG GAGTCCCCAA GCTATAGAAC TTTGAATGGG 
GCGGTGGAGA AGCCATCGCC CCTGCCCCGA TCTGTGGAGG AGTCTTACAT CACCAGTGAG 
CATTGCTACC AGAAACCTCG CGCCTATTAT CCCGCTGTGG AGCAGAGGCT GGTCGTGGAG 
ACGAGAGGCT CTGCCCTTGA TGCTGCAGTC AGCCCCCTCT GTGAGAACGG CGACGACTCC 
CTCTCCCCTC GCCTAGGCTG GCCCATCGAC CAAGACAGGA GCAGAGGCGA CATAGATCCC 
AAACCCAGCT CCCCAAAGGT GAGAGAGTAC ATTTCCAAGA ATGTCTTGCC AGAAGAGACG 
CCTGCAAGGA AGCTGCTGGA CAGAGGTGGA GAAGGGCTGG TGAGCTCTCA GCACCAGTGG 
CAGTTCAACC TGCTCACACA TGTGGAGTCC CTGCAGGATG AGGTGACGCA CCGGATGGAC 
TCCATTGAGA AGGAGCTGGA CGTGCTGGAG AGCTGGCTGG ACTACACTGG GGAGCTGGAG 
CCCCCAGAGC CACTGGCCAG GCTTCCGCAG CTCAAGCACT GCATCAAGCA GCTGCTGACT 
GACCTGGGCA AGGTGCAGCA GATCGCCCTC TGCTGCTCGA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phf20 ( XM_006499338.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499401.1 Mus musculus PHD finger protein 20 (Phf20), transcript variant X2, mRNA.

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CloneID	OMu30368
Clone ID Related Accession (Same CDS sequence)	XM_006499338.3 , XM_006499338.4
Accession Version	XM_006499338.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	PHD finger protein 20 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499338.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAAGC ACCCACCTAA CAGACGAGGA ATCAGCTTTG AAGTGGGAGC CCAGTTGGAA 
GCTCGGGACC GTTTAAAAAA CTGGTATCCA GCTCATATTG AAGACATTGA CTACGAAGAA 
GGGAGAGTTC TCATCCATTT CAAGCGTTGG AACCATCGTT ACGATGAGTG GTTTTGCTGG 
GACAGTCCTT ATCTGCGCCC TTTAGAGAAG ATCCAGCTGA GGAAAGAGGG TTTACATGAT 
GAGGATGGCT CTTCTGAATT TCAAATAAAC CAGCAAGTGC TTGCTTGCTG GTCTGACTGT 
CGATTTTATC CAGCCAGAGT CACTGCTGTG AACAAGGATG CTGTGGATTC AAACTCTCAA 
ACTTTGCAAC CAATAACATT GGAATTGAGA AGACGGAAAA TATCAAAACG AAGTGACACC 
CCATTAAAGC GTCCCAGACT CGACAAAAAT TCACCCCAGG AACAGTCAAA AAAACGCTCT 
GAAAATAGTG ACAAAGACTT ATCCAGGAGA CGGTCCTCCA GGCTGTCCAC TAATGGGACC 
CGTGAGATCC TAGATCCTGA CTCGATTGTA CCTGATCTGG TTCATACGGT TGATACAAAC 
CCTCTACCAG ACAAGTCACC CAGTGCCAAG GATTCTGCTG AAGGTCAGTT GAAGTCTCCA 
TTGGAAGCTG GCCAGGTCTC TTCTGCATTA ACTTGCCACC CCATTGGGGA TGGCCTGGGG 
GCAGCAGATT TGGAGTTGAA TTGCAAGTCA ATGGGAGAAA ACACGATGAA AACAGAACCT 
GTTTCTCCTC TTGCTGAGGT GCAGGAAGTT TCAACTGTTG AAGTTCCAAA TACTTTGAAG 
AAAGTTGATG ACTCTGTGAC GTTGAATGTG CCAGCTGTGG ACCTAGACCA CAAGTTTCGA 
TGCAAGGTTC TGGACTGTTT GAAATTTTTC CGCAAGGCTA AATTGCTGCA CTATCATATG 
AAGTATTTCC ATGGGATGGA GAAGTCACCA GAGCCAGAGG AGGGCCCAGG GAAGACGCAT 
GTACAGACTC GGGGCTCTGC GGTGCCTGAC AAGACAAGCC AGGAGAGCCT AACCAGGAAG 
CGGGTCTCTG CCAGTTCCCC CACTGCAAAA GAGAAGGAAA AGACTAAAGA GAAGAAATTC 
AAAGAACTTG TGAGAGTGAA GCCAAAGAAG AAAAAGAAAA AGAAAAAGAA AACCAAGCCT 
GAATGTCCCT GCAGTGAGGA CATCAGTGAT ACTTCCCAGG AACCTTCTCC ACCCAAAACA 
TTTGCTGTCA CCAGGTGTGG GTCCTCACAC AAGCCTGGGG TCCATATGAG CCCGCAGCTC 
CATGGTTCAG ATAATGGAAA CCACAAAGGG AAATTGAAAA CCTGTGAGGA GGATAATTTG 
AGTGAGTCCT CTTCCGAGAG CTTTCTTTGG AGTGATGAGG AATATGGTCA AGATGTTGAT 
GTGACCACCA ACCCAGATGA GGAGCTTGAG GGCGACGACC GCTATGATTT TGAGGTGGTC 
CGCTGCATCT GTGAAGTGCA GGAGGAAAAT GACTTCATGA TTCAGTGTGA AGAGTGCCAG 
TGCTGGCAGC ACGGGGTCTG CATGGGCTTA CTGGAAGAAA ACGTGCCTGA GAAATACACC 
TGCTATGTTT GCCAAGACCC TCCAGGTCAG AGGCCTGGCT TCAAGTACTG GTATGACAAG 
GAGTGGCTGA GCCGGGGACA CATGCATGGT CTGGCATTTC TGGATCAGAA CTACTCCCAC 
CAGAATGCCA GGAAGATCGT GGCCACCCAC CAGCTGCTCG GGGACGTGCA GAGAGTGATC 
CAAGTGCTGC ACGGCCTGCA GCTCAAGATG AGCATTCTGC AAAGCAGAGA GCATCCTGAT 
CTGCAGCTAT GGTGCCAGCC CTGGAAACAG CACTCGGGGG AGGGAAGAGC ACATCCCAGA 
CACATCCACA TCACAGACGC CAGGAGCGAG GAGTCCCCAA GCTATAGAAC TTTGAATGGG 
GCGGTGGAGA AGCCATCGCC CCTGCCCCGA TCTGTGGAGG AGTCTTACAT CACCAGTGAG 
CATTGCTACC AGAAACCTCG CGCCTATTAT CCCGCTGTGG AGCAGAGGCT GGTCGTGGAG 
ACGAGAGGCT CTGCCCTTGA TGCTGCAGTC AGCCCCCTCT GTGAGAACGG CGACGACTCC 
CTCTCCCCTC GCCTAGGCTG GCCCATCGAC CAAGACAGGA GCAGAGGCGA CATAGATCCC 
AAACCCAGCT CCCCAAAGGT GAGAGAGTAC ATTTCCAAGA ATGTCTTGCC AGAAGAGACG 
CCTGCAAGGA AGCTGCTGGA CAGAGGTGGA GAAGGGCTGG TGAGCTCTCA GCACCAGTGG 
CAGTTCAACC TGCTCACACA TGTGGAGTCC CTGCAGGATG AGGTGACGCA CCGGATGGAC 
TCCATTGAGA AGGAGCTGGA CGTGCTGGAG AGCTGGCTGG ACTACACTGG GGAGCTGGAG 
CCCCCAGAGC CACTGGCCAG GCTTCCGCAG CTCAAGCACT GCATCAAGCA GCTGCTGACT 
GACCTGGGCA AGGTGCAGCA GATCGCCCTC TGCTGCTCGA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499401.1
CDS	166..2730
Translation	MTKHPPNRRGISFEVGAQLEARDRLKNWYPAHIEDIDYEEGRVLIHFKRWNHRYDEWFCWDSPYLRPLEKIQLRKEGLHDEDGSSEFQINQQVLACWSDCRFYPARVTAVNKDAVDSNSQTLQPITLELRRRKISKRSDTPLKRPRLDKNSPQEQSKKRSENSDKDLSRRRSSRLSTNGTREILDPDSIVPDLVHTVDTNPLPDKSPSAKDSAEGQLKSPLEAGQVSSALTCHPIGDGLGAADLELNCKSMGENTMKTEPVSPLAEVQEVSTVEVPNTLKKVDDSVTLNVPAVDLDHKFRCKVLDCLKFFRKAKLLHYHMKYFHGMEKSPEPEEGPGKTHVQTRGSAVPDKTSQESLTRKRVSASSPTAKEKEKTKEKKFKELVRVKPKKKKKKKKKTKPECPCSEDISDTSQEPSPPKTFAVTRCGSSHKPGVHMSPQLHGSDNGNHKGKLKTCEEDNLSESSSESFLWSDEEYGQDVDVTTNPDEELEGDDRYDFEVVRCICEVQEENDFMIQCEECQCWQHGVCMGLLEENVPEKYTCYVCQDPPGQRPGFKYWYDKEWLSRGHMHGLAFLDQNYSHQNARKIVATHQLLGDVQRVIQVLHGLQLKMSILQSREHPDLQLWCQPWKQHSGEGRAHPRHIHITDARSEESPSYRTLNGAVEKPSPLPRSVEESYITSEHCYQKPRAYYPAVEQRLVVETRGSALDAAVSPLCENGDDSLSPRLGWPIDQDRSRGDIDPKPSSPKVREYISKNVLPEETPARKLLDRGGEGLVSSQHQWQFNLLTHVESLQDEVTHRMDSIEKELDVLESWLDYTGELEPPEPLARLPQLKHCIKQLLTDLGKVQQIALCCST

Target ORF information:

RefSeq Version	XM_006499338.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus PHD finger protein 20 (Phf20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499338.4

ORF Insert Sequence:

ATGACCAAGC ACCCACCTAA CAGACGAGGA ATCAGCTTTG AAGTGGGAGC CCAGTTGGAA 
GCTCGGGACC GTTTAAAAAA CTGGTATCCA GCTCATATTG AAGACATTGA CTACGAAGAA 
GGGAGAGTTC TCATCCATTT CAAGCGTTGG AACCATCGTT ACGATGAGTG GTTTTGCTGG 
GACAGTCCTT ATCTGCGCCC TTTAGAGAAG ATCCAGCTGA GGAAAGAGGG TTTACATGAT 
GAGGATGGCT CTTCTGAATT TCAAATAAAC CAGCAAGTGC TTGCTTGCTG GTCTGACTGT 
CGATTTTATC CAGCCAGAGT CACTGCTGTG AACAAGGATG CTGTGGATTC AAACTCTCAA 
ACTTTGCAAC CAATAACATT GGAATTGAGA AGACGGAAAA TATCAAAACG AAGTGACACC 
CCATTAAAGC GTCCCAGACT CGACAAAAAT TCACCCCAGG AACAGTCAAA AAAACGCTCT 
GAAAATAGTG ACAAAGACTT ATCCAGGAGA CGGTCCTCCA GGCTGTCCAC TAATGGGACC 
CGTGAGATCC TAGATCCTGA CTCGATTGTA CCTGATCTGG TTCATACGGT TGATACAAAC 
CCTCTACCAG ACAAGTCACC CAGTGCCAAG GATTCTGCTG AAGGTCAGTT GAAGTCTCCA 
TTGGAAGCTG GCCAGGTCTC TTCTGCATTA ACTTGCCACC CCATTGGGGA TGGCCTGGGG 
GCAGCAGATT TGGAGTTGAA TTGCAAGTCA ATGGGAGAAA ACACGATGAA AACAGAACCT 
GTTTCTCCTC TTGCTGAGGT GCAGGAAGTT TCAACTGTTG AAGTTCCAAA TACTTTGAAG 
AAAGTTGATG ACTCTGTGAC GTTGAATGTG CCAGCTGTGG ACCTAGACCA CAAGTTTCGA 
TGCAAGGTTC TGGACTGTTT GAAATTTTTC CGCAAGGCTA AATTGCTGCA CTATCATATG 
AAGTATTTCC ATGGGATGGA GAAGTCACCA GAGCCAGAGG AGGGCCCAGG GAAGACGCAT 
GTACAGACTC GGGGCTCTGC GGTGCCTGAC AAGACAAGCC AGGAGAGCCT AACCAGGAAG 
CGGGTCTCTG CCAGTTCCCC CACTGCAAAA GAGAAGGAAA AGACTAAAGA GAAGAAATTC 
AAAGAACTTG TGAGAGTGAA GCCAAAGAAG AAAAAGAAAA AGAAAAAGAA AACCAAGCCT 
GAATGTCCCT GCAGTGAGGA CATCAGTGAT ACTTCCCAGG AACCTTCTCC ACCCAAAACA 
TTTGCTGTCA CCAGGTGTGG GTCCTCACAC AAGCCTGGGG TCCATATGAG CCCGCAGCTC 
CATGGTTCAG ATAATGGAAA CCACAAAGGG AAATTGAAAA CCTGTGAGGA GGATAATTTG 
AGTGAGTCCT CTTCCGAGAG CTTTCTTTGG AGTGATGAGG AATATGGTCA AGATGTTGAT 
GTGACCACCA ACCCAGATGA GGAGCTTGAG GGCGACGACC GCTATGATTT TGAGGTGGTC 
CGCTGCATCT GTGAAGTGCA GGAGGAAAAT GACTTCATGA TTCAGTGTGA AGAGTGCCAG 
TGCTGGCAGC ACGGGGTCTG CATGGGCTTA CTGGAAGAAA ACGTGCCTGA GAAATACACC 
TGCTATGTTT GCCAAGACCC TCCAGGTCAG AGGCCTGGCT TCAAGTACTG GTATGACAAG 
GAGTGGCTGA GCCGGGGACA CATGCATGGT CTGGCATTTC TGGATCAGAA CTACTCCCAC 
CAGAATGCCA GGAAGATCGT GGCCACCCAC CAGCTGCTCG GGGACGTGCA GAGAGTGATC 
CAAGTGCTGC ACGGCCTGCA GCTCAAGATG AGCATTCTGC AAAGCAGAGA GCATCCTGAT 
CTGCAGCTAT GGTGCCAGCC CTGGAAACAG CACTCGGGGG AGGGAAGAGC ACATCCCAGA 
CACATCCACA TCACAGACGC CAGGAGCGAG GAGTCCCCAA GCTATAGAAC TTTGAATGGG 
GCGGTGGAGA AGCCATCGCC CCTGCCCCGA TCTGTGGAGG AGTCTTACAT CACCAGTGAG 
CATTGCTACC AGAAACCTCG CGCCTATTAT CCCGCTGTGG AGCAGAGGCT GGTCGTGGAG 
ACGAGAGGCT CTGCCCTTGA TGCTGCAGTC AGCCCCCTCT GTGAGAACGG CGACGACTCC 
CTCTCCCCTC GCCTAGGCTG GCCCATCGAC CAAGACAGGA GCAGAGGCGA CATAGATCCC 
AAACCCAGCT CCCCAAAGGT GAGAGAGTAC ATTTCCAAGA ATGTCTTGCC AGAAGAGACG 
CCTGCAAGGA AGCTGCTGGA CAGAGGTGGA GAAGGGCTGG TGAGCTCTCA GCACCAGTGG 
CAGTTCAACC TGCTCACACA TGTGGAGTCC CTGCAGGATG AGGTGACGCA CCGGATGGAC 
TCCATTGAGA AGGAGCTGGA CGTGCTGGAG AGCTGGCTGG ACTACACTGG GGAGCTGGAG 
CCCCCAGAGC CACTGGCCAG GCTTCCGCAG CTCAAGCACT GCATCAAGCA GCTGCTGACT 
GACCTGGGCA AGGTGCAGCA GATCGCCCTC TGCTGCTCGA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phf20 ( XM_006499339.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499402.1 Mus musculus PHD finger protein 20 (Phf20), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu30369
Clone ID Related Accession (Same CDS sequence)	XM_006499339.1 , XM_006499339.2
Accession Version	XM_006499339.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	PHD finger protein 20 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499339.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAAA ACACGATGAA AACAGAACCT GTTTCTCCTC TTGCTGAGGT GCAGGAAGTT 
TCAACTGTTG AAGTTCCAAA TACTTTGAAG AAAGTTGATG ACTCTGTGAC GTTGAATGTG 
CCAGCTGTGG ACCTAGACCA CAAGTTTCGA TGCAAGGTTC TGGACTGTTT GAAATTTTTC 
CGCAAGGCTA AATTGCTGCA CTATCATATG AAGTATTTCC ATGGGATGGA GAAGTCACCA 
GAGCCAGAGG AGGGCCCAGG GAAGACGCAT GTACAGACTC GGGGCTCTGC GGTGCCTGAC 
AAGACAAGCC AGGAGAGCCT AACCAGGAAG CGGGTCTCTG CCAGTTCCCC CACTGCAAAA 
GAGAAGGAAA AGACTAAAGA GAAGAAATTC AAAGAACTTG TGAGAGTGAA GCCAAAGAAG 
AAAAAGAAAA AGAAAAAGAA AACCAAGCCT GAATGTCCCT GCAGTGAGGA CATCAGTGAT 
ACTTCCCAGG AACCTTCTCC ACCCAAAACA TTTGCTGTCA CCAGGTGTGG GTCCTCACAC 
AAGCCTGGGG TCCATATGAG CCCGCAGCTC CATGGTTCAG ATAATGGAAA CCACAAAGGG 
AAATTGAAAA CCTGTGAGGA GGATAATTTG AGTGAGTCCT CTTCCGAGAG CTTTCTTTGG 
AGTGATGAGG AATATGGTCA AGATGTTGAT GTGACCACCA ACCCAGATGA GGAGCTTGAG 
GGCGACGACC GCTATGATTT TGAGGTGGTC CGCTGCATCT GTGAAGTGCA GGAGGAAAAT 
GACTTCATGA TTCAGTGTGA AGAGTGCCAG TGCTGGCAGC ACGGGGTCTG CATGGGCTTA 
CTGGAAGAAA ACGTGCCTGA GAAATACACC TGCTATGTTT GCCAAGACCC TCCAGGTCAG 
AGGCCTGGCT TCAAGTACTG GTATGACAAG GAGTGGCTGA GCCGGGGACA CATGCATGGT 
CTGGCATTTC TGGATCAGAA CTACTCCCAC CAGAATGCCA GGAAGATCGT GGCCACCCAC 
CAGCTGCTCG GGGACGTGCA GAGAGTGATC CAAGTGCTGC ACGGCCTGCA GCTCAAGATG 
AGCATTCTGC AAAGCAGAGA GCATCCTGAT CTGCAGCTAT GGTGCCAGCC CTGGAAACAG 
CACTCGGGGG AGGGAAGAGC ACATCCCAGA CACATCCACA TCACAGACGC CAGGAGCGAG 
GAGTCCCCAA GCTATAGAAC TTTGAATGGG GCGGTGGAGA AGCCATCGCC CCTGCCCCGA 
TCTGTGGAGG AGTCTTACAT CACCAGTGAG CATTGCTACC AGAAACCTCG CGCCTATTAT 
CCCGCTGTGG AGCAGAGGCT GGTCGTGGAG ACGAGAGGCT CTGCCCTTGA TGCTGCAGTC 
AGCCCCCTCT GTGAGAACGG CGACGACTCC CTCTCCCCTC GCCTAGGCTG GCCCATCGAC 
CAAGACAGGA GCAGAGGCGA CATAGATCCC AAACCCAGCT CCCCAAAGGT GAGAGAGTAC 
ATTTCCAAGA ATGTCTTGCC AGAAGAGACG CCTGCAAGGA AGCTGCTGGA CAGAGGTGGA 
GAAGGGCTGG TGAGCTCTCA GCACCAGTGG CAGTTCAACC TGCTCACACA TGTGGAGTCC 
CTGCAGGATG AGGTGACGCA CCGGATGGAC TCCATTGAGA AGGAGCTGGA CGTGCTGGAG 
AGCTGGCTGG ACTACACTGG GGAGCTGGAG CCCCCAGAGC CACTGGCCAG GCTTCCGCAG 
CTCAAGCACT GCATCAAGCA GCTGCTGACT GACCTGGGCA AGGTGCAGCA GATCGCCCTC 
TGCTGCTCGA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499402.1
CDS	1251..3065
Translation	MGENTMKTEPVSPLAEVQEVSTVEVPNTLKKVDDSVTLNVPAVDLDHKFRCKVLDCLKFFRKAKLLHYHMKYFHGMEKSPEPEEGPGKTHVQTRGSAVPDKTSQESLTRKRVSASSPTAKEKEKTKEKKFKELVRVKPKKKKKKKKKTKPECPCSEDISDTSQEPSPPKTFAVTRCGSSHKPGVHMSPQLHGSDNGNHKGKLKTCEEDNLSESSSESFLWSDEEYGQDVDVTTNPDEELEGDDRYDFEVVRCICEVQEENDFMIQCEECQCWQHGVCMGLLEENVPEKYTCYVCQDPPGQRPGFKYWYDKEWLSRGHMHGLAFLDQNYSHQNARKIVATHQLLGDVQRVIQVLHGLQLKMSILQSREHPDLQLWCQPWKQHSGEGRAHPRHIHITDARSEESPSYRTLNGAVEKPSPLPRSVEESYITSEHCYQKPRAYYPAVEQRLVVETRGSALDAAVSPLCENGDDSLSPRLGWPIDQDRSRGDIDPKPSSPKVREYISKNVLPEETPARKLLDRGGEGLVSSQHQWQFNLLTHVESLQDEVTHRMDSIEKELDVLESWLDYTGELEPPEPLARLPQLKHCIKQLLTDLGKVQQIALCCST

Target ORF information:

RefSeq Version	XM_006499339.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus PHD finger protein 20 (Phf20), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499339.2

ORF Insert Sequence:

ATGGGAGAAA ACACGATGAA AACAGAACCT GTTTCTCCTC TTGCTGAGGT GCAGGAAGTT 
TCAACTGTTG AAGTTCCAAA TACTTTGAAG AAAGTTGATG ACTCTGTGAC GTTGAATGTG 
CCAGCTGTGG ACCTAGACCA CAAGTTTCGA TGCAAGGTTC TGGACTGTTT GAAATTTTTC 
CGCAAGGCTA AATTGCTGCA CTATCATATG AAGTATTTCC ATGGGATGGA GAAGTCACCA 
GAGCCAGAGG AGGGCCCAGG GAAGACGCAT GTACAGACTC GGGGCTCTGC GGTGCCTGAC 
AAGACAAGCC AGGAGAGCCT AACCAGGAAG CGGGTCTCTG CCAGTTCCCC CACTGCAAAA 
GAGAAGGAAA AGACTAAAGA GAAGAAATTC AAAGAACTTG TGAGAGTGAA GCCAAAGAAG 
AAAAAGAAAA AGAAAAAGAA AACCAAGCCT GAATGTCCCT GCAGTGAGGA CATCAGTGAT 
ACTTCCCAGG AACCTTCTCC ACCCAAAACA TTTGCTGTCA CCAGGTGTGG GTCCTCACAC 
AAGCCTGGGG TCCATATGAG CCCGCAGCTC CATGGTTCAG ATAATGGAAA CCACAAAGGG 
AAATTGAAAA CCTGTGAGGA GGATAATTTG AGTGAGTCCT CTTCCGAGAG CTTTCTTTGG 
AGTGATGAGG AATATGGTCA AGATGTTGAT GTGACCACCA ACCCAGATGA GGAGCTTGAG 
GGCGACGACC GCTATGATTT TGAGGTGGTC CGCTGCATCT GTGAAGTGCA GGAGGAAAAT 
GACTTCATGA TTCAGTGTGA AGAGTGCCAG TGCTGGCAGC ACGGGGTCTG CATGGGCTTA 
CTGGAAGAAA ACGTGCCTGA GAAATACACC TGCTATGTTT GCCAAGACCC TCCAGGTCAG 
AGGCCTGGCT TCAAGTACTG GTATGACAAG GAGTGGCTGA GCCGGGGACA CATGCATGGT 
CTGGCATTTC TGGATCAGAA CTACTCCCAC CAGAATGCCA GGAAGATCGT GGCCACCCAC 
CAGCTGCTCG GGGACGTGCA GAGAGTGATC CAAGTGCTGC ACGGCCTGCA GCTCAAGATG 
AGCATTCTGC AAAGCAGAGA GCATCCTGAT CTGCAGCTAT GGTGCCAGCC CTGGAAACAG 
CACTCGGGGG AGGGAAGAGC ACATCCCAGA CACATCCACA TCACAGACGC CAGGAGCGAG 
GAGTCCCCAA GCTATAGAAC TTTGAATGGG GCGGTGGAGA AGCCATCGCC CCTGCCCCGA 
TCTGTGGAGG AGTCTTACAT CACCAGTGAG CATTGCTACC AGAAACCTCG CGCCTATTAT 
CCCGCTGTGG AGCAGAGGCT GGTCGTGGAG ACGAGAGGCT CTGCCCTTGA TGCTGCAGTC 
AGCCCCCTCT GTGAGAACGG CGACGACTCC CTCTCCCCTC GCCTAGGCTG GCCCATCGAC 
CAAGACAGGA GCAGAGGCGA CATAGATCCC AAACCCAGCT CCCCAAAGGT GAGAGAGTAC 
ATTTCCAAGA ATGTCTTGCC AGAAGAGACG CCTGCAAGGA AGCTGCTGGA CAGAGGTGGA 
GAAGGGCTGG TGAGCTCTCA GCACCAGTGG CAGTTCAACC TGCTCACACA TGTGGAGTCC 
CTGCAGGATG AGGTGACGCA CCGGATGGAC TCCATTGAGA AGGAGCTGGA CGTGCTGGAG 
AGCTGGCTGG ACTACACTGG GGAGCTGGAG CCCCCAGAGC CACTGGCCAG GCTTCCGCAG 
CTCAAGCACT GCATCAAGCA GCTGCTGACT GACCTGGGCA AGGTGCAGCA GATCGCCCTC 
TGCTGCTCGA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phf20 ( XM_017317827.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173316.1 Mus musculus PHD finger protein 20 (Phf20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu80233
Clone ID Related Accession (Same CDS sequence)	XM_017317827.1 , XM_017317827.2
Accession Version	XM_017317827.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	PHD finger protein 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAAGC ACCCACCTAA CAGACGAGGA ATCAGCTTTG AAGTGGGAGC CCAGTTGGAA 
GCTCGGGACC GTTTAAAAAA CTGGTATCCA GCTCATATTG AAGACATTGA CTACGAAGAA 
GGGAGAGTTC TCATCCATTT CAAGCGTTGG AACCATCGTT ACGATGAGTG GTTTTGCTGG 
GACAGTCCTT ATCTGCGCCC TTTAGAGAAG ATCCAGCTGA GGAAAGAGGG TTTACATGAT 
GAGGATGGCT CTTCTAATAT TGTGGGTAAT GCTAGGCCTA AAGAAACAGA CCACAAAAGT 
CTTTCATCGT CTCCTGAGAA ACGAGAGAAG TTTAAAGAAC AGAGAAAAGT CACGGTCAAT 
GTGAAGAAAG ACAAAGTGGA AAAAGCCTTA AAGACAGAAA AGCGGCCCAA GCAACCTGAC 
AAAGAGGGAA AGCTGATCTG CTCAGAAAAA GGCAAAGTGT CAGAGAAAAG CCTTCCTAAG 
AACGAAAAGG AAGATAAGGA GAACATTTCC GAGAACGAGC GGGAGTACTC CGGGGATGCC 
CAGGTGGAAA AGAAGCCTGA GAAGGACCTT GTGAAGAACC CACAAGAGAA CCTGAAGGAG 
CCAAAAAGAA AACGAGGCAG ACCCCCTTCC ATAACTCCTA CGGCTGTGGA TTCAAACTCT 
CAAACTTTGC AACCAATAAC ATTGGAATTG AGAAGACGGA AAATATCAAA ACGAAGTGAC 
ACCCCATTAA AGCGTCCCAG ACTCGACAAA AATTCACCCC AGGAACAGTC AAAAAAACGC 
TCTGAAAATA GTGACAAAGA CTTATCCAGG AGACGGTCCT CCAGGCTGTC CACTAATGGG 
ACCCGTGAGA TCCTAGATCC TGACTCGATT GTACCTGATC TGGTTCATAC GGTTGATACA 
AACCCTCTAC CAGACAAGTC ACCCAGTGCC AAGGATTCTG CTGAAGGTCA GTTGAAGTCT 
CCATTGGAAG CTGGCCAGGT CTCTTCTGCA TTAACTTGCC ACCCCATTGG GGATGGCCTG 
GGGGCAGCAG ATTTGGAGTT GAATTGCAAG TCAATGGGAG AAAACACGAT GAAAACAGAA 
CCTGTTTCTC CTCTTGCTGA GGTGCAGGAA GTTTCAACTG TTGAAGTTCC AAATACTTTG 
AAGAAAGTTG ATGACTCTGT GACGTTGAAT GTGCCAGCTG TGGACCTAGA CCACAAGTTT 
CGATGCAAGG TTCTGGACTG TTTGAAATTT TTCCGCAAGG CTAAATTGCT GCACTATCAT 
ATGAAGTATT TCCATGGGAT GGAGAAGTCA CCAGAGCCAG AGGAGGGCCC AGGGAAGACG 
CATGTACAGA CTCGGGGCTC TGCGGTGCCT GACAAGACAA GCCAGGAGAG CCTAACCAGG 
AAGCGGGTCT CTGCCAGTTC CCCCACTGCA AAAGAGAAGG AAAAGACTAA AGAGAAGAAA 
TTCAAAGAAC TTGTGAGAGT GAAGCCAAAG AAGAAAAAGA AAAAGAAAAA GAAAACCAAG 
CCTGAATGTC CCTGCAGTGA GGACATCAGT GATACTTCCC AGGAACCTTC TCCACCCAAA 
ACATTTGCTG TCACCAGGTG TGGGTCCTCA CACAAGCCTG GGGTCCATAT GAGCCCGCAG 
CTCCATGGTT CAGATAATGG AAACCACAAA GGGAAATTGA AAACCTGTGA GGAGGATAAT 
TTGAGTGAGT CCTCTTCCGA GAGCTTTCTT TGGAGTGATG AGGAATATGG TCAAGATGTT 
GATGTGACCA CCAACCCAGA TGAGGAGCTT GAGGGCGACG ACCGCTATGA TTTTGAGGTG 
GTCCGCTGCA TCTGTGAAGT GCAGGAGGAA AATGACTTCA TGATTCAGTG TGAAGAGTGC 
CAGTGCTGGC AGCACGGGGT CTGCATGGGC TTACTGGAAG AAAACGTGCC TGAGAAATAC 
ACCTGCTATG TTTGCCAAGA CCCTCCAGGT CAGAGGCCTG GCTTCAAGTA CTGGTATGAC 
AAGGAGTGGC TGAGCCGGGG ACACATGCAT GGTCTGGCAT TTCTGGATCA GAACTACTCC 
CACCAGAATG CCAGGAAGAT CGTGGCCACC CACCAGCTGC TCGGGGACGT GCAGAGAGTG 
ATCCAAGTGC TGCACGGCCT GCAGCTCAAG ATGAGCATTC TGCAAAGCAG AGAGCATCCT 
GATCTGCAGC TATGGTGCCA GCCCTGGAAA CAGCACTCGG GGGAGGGAAG AGCACATCCC 
AGACACATCC ACATCACAGA CGCCAGGAGC GAGGAGTCCC CAAGCTATAG AACTTTGAAT 
GGGGCGGTGG AGAAGCCATC GCCCCTGCCC CGATCTGTGG AGGAGTCTTA CATCACCAGT 
GAGCATTGCT ACCAGAAACC TCGCGCCTAT TATCCCGCTG TGGAGCAGAG GCTGGTCGTG 
GAGACGAGAG GCTCTGCCCT TGATGCTGCA GTCAGCCCCC TCTGTGAGAA CGGCGACGAC 
TCCCTCTCCC CTCGCCTAGG CTGGCCCATC GACCAAGACA GGAGCAGAGG CGACATAGAT 
CCCAAACCCA GCTCCCCAAA GGTGAGAGAG TACATTTCCA AGAATGTCTT GCCAGAAGAG 
ACGCCTGCAA GGAAGCTGCT GGACAGAGGT GGAGAAGGGC TGGTGAGCTC TCAGCACCAG 
TGGCAGTTCA ACCTGCTCAC ACATGTGGAG TCCCTGCAGG ATGAGGTGAC GCACCGGATG 
GACTCCATTG AGAAGGAGCT GGACGTGCTG GAGAGCTGGC TGGACTACAC TGGGGAGCTG 
GAGCCCCCAG AGCCACTGGC CAGGCTTCCG CAGCTCAAGC ACTGCATCAA GCAGCTGCTG 
ACTGACCTGG GCAAGGTGCA GCAGATCGCC CTCTGCTGCT CGACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173316.1
CDS	118..2985
Translation	MTKHPPNRRGISFEVGAQLEARDRLKNWYPAHIEDIDYEEGRVLIHFKRWNHRYDEWFCWDSPYLRPLEKIQLRKEGLHDEDGSSNIVGNARPKETDHKSLSSSPEKREKFKEQRKVTVNVKKDKVEKALKTEKRPKQPDKEGKLICSEKGKVSEKSLPKNEKEDKENISENEREYSGDAQVEKKPEKDLVKNPQENLKEPKRKRGRPPSITPTAVDSNSQTLQPITLELRRRKISKRSDTPLKRPRLDKNSPQEQSKKRSENSDKDLSRRRSSRLSTNGTREILDPDSIVPDLVHTVDTNPLPDKSPSAKDSAEGQLKSPLEAGQVSSALTCHPIGDGLGAADLELNCKSMGENTMKTEPVSPLAEVQEVSTVEVPNTLKKVDDSVTLNVPAVDLDHKFRCKVLDCLKFFRKAKLLHYHMKYFHGMEKSPEPEEGPGKTHVQTRGSAVPDKTSQESLTRKRVSASSPTAKEKEKTKEKKFKELVRVKPKKKKKKKKKTKPECPCSEDISDTSQEPSPPKTFAVTRCGSSHKPGVHMSPQLHGSDNGNHKGKLKTCEEDNLSESSSESFLWSDEEYGQDVDVTTNPDEELEGDDRYDFEVVRCICEVQEENDFMIQCEECQCWQHGVCMGLLEENVPEKYTCYVCQDPPGQRPGFKYWYDKEWLSRGHMHGLAFLDQNYSHQNARKIVATHQLLGDVQRVIQVLHGLQLKMSILQSREHPDLQLWCQPWKQHSGEGRAHPRHIHITDARSEESPSYRTLNGAVEKPSPLPRSVEESYITSEHCYQKPRAYYPAVEQRLVVETRGSALDAAVSPLCENGDDSLSPRLGWPIDQDRSRGDIDPKPSSPKVREYISKNVLPEETPARKLLDRGGEGLVSSQHQWQFNLLTHVESLQDEVTHRMDSIEKELDVLESWLDYTGELEPPEPLARLPQLKHCIKQLLTDLGKVQQIALCCST

Target ORF information:

RefSeq Version	XM_017317827.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus PHD finger protein 20 (Phf20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317827.1

ORF Insert Sequence:

ATGACCAAGC ACCCACCTAA CAGACGAGGA ATCAGCTTTG AAGTGGGAGC CCAGTTGGAA 
GCTCGGGACC GTTTAAAAAA CTGGTATCCA GCTCATATTG AAGACATTGA CTACGAAGAA 
GGGAGAGTTC TCATCCATTT CAAGCGTTGG AACCATCGTT ACGATGAGTG GTTTTGCTGG 
GACAGTCCTT ATCTGCGCCC TTTAGAGAAG ATCCAGCTGA GGAAAGAGGG TTTACATGAT 
GAGGATGGCT CTTCTAATAT TGTGGGTAAT GCTAGGCCTA AAGAAACAGA CCACAAAAGT 
CTTTCATCGT CTCCTGAGAA ACGAGAGAAG TTTAAAGAAC AGAGAAAAGT CACGGTCAAT 
GTGAAGAAAG ACAAAGTGGA AAAAGCCTTA AAGACAGAAA AGCGGCCCAA GCAACCTGAC 
AAAGAGGGAA AGCTGATCTG CTCAGAAAAA GGCAAAGTGT CAGAGAAAAG CCTTCCTAAG 
AACGAAAAGG AAGATAAGGA GAACATTTCC GAGAACGAGC GGGAGTACTC CGGGGATGCC 
CAGGTGGAAA AGAAGCCTGA GAAGGACCTT GTGAAGAACC CACAAGAGAA CCTGAAGGAG 
CCAAAAAGAA AACGAGGCAG ACCCCCTTCC ATAACTCCTA CGGCTGTGGA TTCAAACTCT 
CAAACTTTGC AACCAATAAC ATTGGAATTG AGAAGACGGA AAATATCAAA ACGAAGTGAC 
ACCCCATTAA AGCGTCCCAG ACTCGACAAA AATTCACCCC AGGAACAGTC AAAAAAACGC 
TCTGAAAATA GTGACAAAGA CTTATCCAGG AGACGGTCCT CCAGGCTGTC CACTAATGGG 
ACCCGTGAGA TCCTAGATCC TGACTCGATT GTACCTGATC TGGTTCATAC GGTTGATACA 
AACCCTCTAC CAGACAAGTC ACCCAGTGCC AAGGATTCTG CTGAAGGTCA GTTGAAGTCT 
CCATTGGAAG CTGGCCAGGT CTCTTCTGCA TTAACTTGCC ACCCCATTGG GGATGGCCTG 
GGGGCAGCAG ATTTGGAGTT GAATTGCAAG TCAATGGGAG AAAACACGAT GAAAACAGAA 
CCTGTTTCTC CTCTTGCTGA GGTGCAGGAA GTTTCAACTG TTGAAGTTCC AAATACTTTG 
AAGAAAGTTG ATGACTCTGT GACGTTGAAT GTGCCAGCTG TGGACCTAGA CCACAAGTTT 
CGATGCAAGG TTCTGGACTG TTTGAAATTT TTCCGCAAGG CTAAATTGCT GCACTATCAT 
ATGAAGTATT TCCATGGGAT GGAGAAGTCA CCAGAGCCAG AGGAGGGCCC AGGGAAGACG 
CATGTACAGA CTCGGGGCTC TGCGGTGCCT GACAAGACAA GCCAGGAGAG CCTAACCAGG 
AAGCGGGTCT CTGCCAGTTC CCCCACTGCA AAAGAGAAGG AAAAGACTAA AGAGAAGAAA 
TTCAAAGAAC TTGTGAGAGT GAAGCCAAAG AAGAAAAAGA AAAAGAAAAA GAAAACCAAG 
CCTGAATGTC CCTGCAGTGA GGACATCAGT GATACTTCCC AGGAACCTTC TCCACCCAAA 
ACATTTGCTG TCACCAGGTG TGGGTCCTCA CACAAGCCTG GGGTCCATAT GAGCCCGCAG 
CTCCATGGTT CAGATAATGG AAACCACAAA GGGAAATTGA AAACCTGTGA GGAGGATAAT 
TTGAGTGAGT CCTCTTCCGA GAGCTTTCTT TGGAGTGATG AGGAATATGG TCAAGATGTT 
GATGTGACCA CCAACCCAGA TGAGGAGCTT GAGGGCGACG ACCGCTATGA TTTTGAGGTG 
GTCCGCTGCA TCTGTGAAGT GCAGGAGGAA AATGACTTCA TGATTCAGTG TGAAGAGTGC 
CAGTGCTGGC AGCACGGGGT CTGCATGGGC TTACTGGAAG AAAACGTGCC TGAGAAATAC 
ACCTGCTATG TTTGCCAAGA CCCTCCAGGT CAGAGGCCTG GCTTCAAGTA CTGGTATGAC 
AAGGAGTGGC TGAGCCGGGG ACACATGCAT GGTCTGGCAT TTCTGGATCA GAACTACTCC 
CACCAGAATG CCAGGAAGAT CGTGGCCACC CACCAGCTGC TCGGGGACGT GCAGAGAGTG 
ATCCAAGTGC TGCACGGCCT GCAGCTCAAG ATGAGCATTC TGCAAAGCAG AGAGCATCCT 
GATCTGCAGC TATGGTGCCA GCCCTGGAAA CAGCACTCGG GGGAGGGAAG AGCACATCCC 
AGACACATCC ACATCACAGA CGCCAGGAGC GAGGAGTCCC CAAGCTATAG AACTTTGAAT 
GGGGCGGTGG AGAAGCCATC GCCCCTGCCC CGATCTGTGG AGGAGTCTTA CATCACCAGT 
GAGCATTGCT ACCAGAAACC TCGCGCCTAT TATCCCGCTG TGGAGCAGAG GCTGGTCGTG 
GAGACGAGAG GCTCTGCCCT TGATGCTGCA GTCAGCCCCC TCTGTGAGAA CGGCGACGAC 
TCCCTCTCCC CTCGCCTAGG CTGGCCCATC GACCAAGACA GGAGCAGAGG CGACATAGAT 
CCCAAACCCA GCTCCCCAAA GGTGAGAGAG TACATTTCCA AGAATGTCTT GCCAGAAGAG 
ACGCCTGCAA GGAAGCTGCT GGACAGAGGT GGAGAAGGGC TGGTGAGCTC TCAGCACCAG 
TGGCAGTTCA ACCTGCTCAC ACATGTGGAG TCCCTGCAGG ATGAGGTGAC GCACCGGATG 
GACTCCATTG AGAAGGAGCT GGACGTGCTG GAGAGCTGGC TGGACTACAC TGGGGAGCTG 
GAGCCCCCAG AGCCACTGGC CAGGCTTCCG CAGCTCAAGC ACTGCATCAA GCAGCTGCTG 
ACTGACCTGG GCAAGGTGCA GCAGATCGCC CTCTGCTGCT CGACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phf20 ( XM_017317827.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173316.1 Mus musculus PHD finger protein 20 (Phf20), transcript variant X1, mRNA.

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CloneID	OMu80233
Clone ID Related Accession (Same CDS sequence)	XM_017317827.1 , XM_017317827.2
Accession Version	XM_017317827.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	PHD finger protein 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317827.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAAGC ACCCACCTAA CAGACGAGGA ATCAGCTTTG AAGTGGGAGC CCAGTTGGAA 
GCTCGGGACC GTTTAAAAAA CTGGTATCCA GCTCATATTG AAGACATTGA CTACGAAGAA 
GGGAGAGTTC TCATCCATTT CAAGCGTTGG AACCATCGTT ACGATGAGTG GTTTTGCTGG 
GACAGTCCTT ATCTGCGCCC TTTAGAGAAG ATCCAGCTGA GGAAAGAGGG TTTACATGAT 
GAGGATGGCT CTTCTAATAT TGTGGGTAAT GCTAGGCCTA AAGAAACAGA CCACAAAAGT 
CTTTCATCGT CTCCTGAGAA ACGAGAGAAG TTTAAAGAAC AGAGAAAAGT CACGGTCAAT 
GTGAAGAAAG ACAAAGTGGA AAAAGCCTTA AAGACAGAAA AGCGGCCCAA GCAACCTGAC 
AAAGAGGGAA AGCTGATCTG CTCAGAAAAA GGCAAAGTGT CAGAGAAAAG CCTTCCTAAG 
AACGAAAAGG AAGATAAGGA GAACATTTCC GAGAACGAGC GGGAGTACTC CGGGGATGCC 
CAGGTGGAAA AGAAGCCTGA GAAGGACCTT GTGAAGAACC CACAAGAGAA CCTGAAGGAG 
CCAAAAAGAA AACGAGGCAG ACCCCCTTCC ATAACTCCTA CGGCTGTGGA TTCAAACTCT 
CAAACTTTGC AACCAATAAC ATTGGAATTG AGAAGACGGA AAATATCAAA ACGAAGTGAC 
ACCCCATTAA AGCGTCCCAG ACTCGACAAA AATTCACCCC AGGAACAGTC AAAAAAACGC 
TCTGAAAATA GTGACAAAGA CTTATCCAGG AGACGGTCCT CCAGGCTGTC CACTAATGGG 
ACCCGTGAGA TCCTAGATCC TGACTCGATT GTACCTGATC TGGTTCATAC GGTTGATACA 
AACCCTCTAC CAGACAAGTC ACCCAGTGCC AAGGATTCTG CTGAAGGTCA GTTGAAGTCT 
CCATTGGAAG CTGGCCAGGT CTCTTCTGCA TTAACTTGCC ACCCCATTGG GGATGGCCTG 
GGGGCAGCAG ATTTGGAGTT GAATTGCAAG TCAATGGGAG AAAACACGAT GAAAACAGAA 
CCTGTTTCTC CTCTTGCTGA GGTGCAGGAA GTTTCAACTG TTGAAGTTCC AAATACTTTG 
AAGAAAGTTG ATGACTCTGT GACGTTGAAT GTGCCAGCTG TGGACCTAGA CCACAAGTTT 
CGATGCAAGG TTCTGGACTG TTTGAAATTT TTCCGCAAGG CTAAATTGCT GCACTATCAT 
ATGAAGTATT TCCATGGGAT GGAGAAGTCA CCAGAGCCAG AGGAGGGCCC AGGGAAGACG 
CATGTACAGA CTCGGGGCTC TGCGGTGCCT GACAAGACAA GCCAGGAGAG CCTAACCAGG 
AAGCGGGTCT CTGCCAGTTC CCCCACTGCA AAAGAGAAGG AAAAGACTAA AGAGAAGAAA 
TTCAAAGAAC TTGTGAGAGT GAAGCCAAAG AAGAAAAAGA AAAAGAAAAA GAAAACCAAG 
CCTGAATGTC CCTGCAGTGA GGACATCAGT GATACTTCCC AGGAACCTTC TCCACCCAAA 
ACATTTGCTG TCACCAGGTG TGGGTCCTCA CACAAGCCTG GGGTCCATAT GAGCCCGCAG 
CTCCATGGTT CAGATAATGG AAACCACAAA GGGAAATTGA AAACCTGTGA GGAGGATAAT 
TTGAGTGAGT CCTCTTCCGA GAGCTTTCTT TGGAGTGATG AGGAATATGG TCAAGATGTT 
GATGTGACCA CCAACCCAGA TGAGGAGCTT GAGGGCGACG ACCGCTATGA TTTTGAGGTG 
GTCCGCTGCA TCTGTGAAGT GCAGGAGGAA AATGACTTCA TGATTCAGTG TGAAGAGTGC 
CAGTGCTGGC AGCACGGGGT CTGCATGGGC TTACTGGAAG AAAACGTGCC TGAGAAATAC 
ACCTGCTATG TTTGCCAAGA CCCTCCAGGT CAGAGGCCTG GCTTCAAGTA CTGGTATGAC 
AAGGAGTGGC TGAGCCGGGG ACACATGCAT GGTCTGGCAT TTCTGGATCA GAACTACTCC 
CACCAGAATG CCAGGAAGAT CGTGGCCACC CACCAGCTGC TCGGGGACGT GCAGAGAGTG 
ATCCAAGTGC TGCACGGCCT GCAGCTCAAG ATGAGCATTC TGCAAAGCAG AGAGCATCCT 
GATCTGCAGC TATGGTGCCA GCCCTGGAAA CAGCACTCGG GGGAGGGAAG AGCACATCCC 
AGACACATCC ACATCACAGA CGCCAGGAGC GAGGAGTCCC CAAGCTATAG AACTTTGAAT 
GGGGCGGTGG AGAAGCCATC GCCCCTGCCC CGATCTGTGG AGGAGTCTTA CATCACCAGT 
GAGCATTGCT ACCAGAAACC TCGCGCCTAT TATCCCGCTG TGGAGCAGAG GCTGGTCGTG 
GAGACGAGAG GCTCTGCCCT TGATGCTGCA GTCAGCCCCC TCTGTGAGAA CGGCGACGAC 
TCCCTCTCCC CTCGCCTAGG CTGGCCCATC GACCAAGACA GGAGCAGAGG CGACATAGAT 
CCCAAACCCA GCTCCCCAAA GGTGAGAGAG TACATTTCCA AGAATGTCTT GCCAGAAGAG 
ACGCCTGCAA GGAAGCTGCT GGACAGAGGT GGAGAAGGGC TGGTGAGCTC TCAGCACCAG 
TGGCAGTTCA ACCTGCTCAC ACATGTGGAG TCCCTGCAGG ATGAGGTGAC GCACCGGATG 
GACTCCATTG AGAAGGAGCT GGACGTGCTG GAGAGCTGGC TGGACTACAC TGGGGAGCTG 
GAGCCCCCAG AGCCACTGGC CAGGCTTCCG CAGCTCAAGC ACTGCATCAA GCAGCTGCTG 
ACTGACCTGG GCAAGGTGCA GCAGATCGCC CTCTGCTGCT CGACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173316.1
CDS	118..2985
Translation	MTKHPPNRRGISFEVGAQLEARDRLKNWYPAHIEDIDYEEGRVLIHFKRWNHRYDEWFCWDSPYLRPLEKIQLRKEGLHDEDGSSNIVGNARPKETDHKSLSSSPEKREKFKEQRKVTVNVKKDKVEKALKTEKRPKQPDKEGKLICSEKGKVSEKSLPKNEKEDKENISENEREYSGDAQVEKKPEKDLVKNPQENLKEPKRKRGRPPSITPTAVDSNSQTLQPITLELRRRKISKRSDTPLKRPRLDKNSPQEQSKKRSENSDKDLSRRRSSRLSTNGTREILDPDSIVPDLVHTVDTNPLPDKSPSAKDSAEGQLKSPLEAGQVSSALTCHPIGDGLGAADLELNCKSMGENTMKTEPVSPLAEVQEVSTVEVPNTLKKVDDSVTLNVPAVDLDHKFRCKVLDCLKFFRKAKLLHYHMKYFHGMEKSPEPEEGPGKTHVQTRGSAVPDKTSQESLTRKRVSASSPTAKEKEKTKEKKFKELVRVKPKKKKKKKKKTKPECPCSEDISDTSQEPSPPKTFAVTRCGSSHKPGVHMSPQLHGSDNGNHKGKLKTCEEDNLSESSSESFLWSDEEYGQDVDVTTNPDEELEGDDRYDFEVVRCICEVQEENDFMIQCEECQCWQHGVCMGLLEENVPEKYTCYVCQDPPGQRPGFKYWYDKEWLSRGHMHGLAFLDQNYSHQNARKIVATHQLLGDVQRVIQVLHGLQLKMSILQSREHPDLQLWCQPWKQHSGEGRAHPRHIHITDARSEESPSYRTLNGAVEKPSPLPRSVEESYITSEHCYQKPRAYYPAVEQRLVVETRGSALDAAVSPLCENGDDSLSPRLGWPIDQDRSRGDIDPKPSSPKVREYISKNVLPEETPARKLLDRGGEGLVSSQHQWQFNLLTHVESLQDEVTHRMDSIEKELDVLESWLDYTGELEPPEPLARLPQLKHCIKQLLTDLGKVQQIALCCST

Target ORF information:

RefSeq Version	XM_017317827.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus PHD finger protein 20 (Phf20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317827.2

ORF Insert Sequence:

ATGACCAAGC ACCCACCTAA CAGACGAGGA ATCAGCTTTG AAGTGGGAGC CCAGTTGGAA 
GCTCGGGACC GTTTAAAAAA CTGGTATCCA GCTCATATTG AAGACATTGA CTACGAAGAA 
GGGAGAGTTC TCATCCATTT CAAGCGTTGG AACCATCGTT ACGATGAGTG GTTTTGCTGG 
GACAGTCCTT ATCTGCGCCC TTTAGAGAAG ATCCAGCTGA GGAAAGAGGG TTTACATGAT 
GAGGATGGCT CTTCTAATAT TGTGGGTAAT GCTAGGCCTA AAGAAACAGA CCACAAAAGT 
CTTTCATCGT CTCCTGAGAA ACGAGAGAAG TTTAAAGAAC AGAGAAAAGT CACGGTCAAT 
GTGAAGAAAG ACAAAGTGGA AAAAGCCTTA AAGACAGAAA AGCGGCCCAA GCAACCTGAC 
AAAGAGGGAA AGCTGATCTG CTCAGAAAAA GGCAAAGTGT CAGAGAAAAG CCTTCCTAAG 
AACGAAAAGG AAGATAAGGA GAACATTTCC GAGAACGAGC GGGAGTACTC CGGGGATGCC 
CAGGTGGAAA AGAAGCCTGA GAAGGACCTT GTGAAGAACC CACAAGAGAA CCTGAAGGAG 
CCAAAAAGAA AACGAGGCAG ACCCCCTTCC ATAACTCCTA CGGCTGTGGA TTCAAACTCT 
CAAACTTTGC AACCAATAAC ATTGGAATTG AGAAGACGGA AAATATCAAA ACGAAGTGAC 
ACCCCATTAA AGCGTCCCAG ACTCGACAAA AATTCACCCC AGGAACAGTC AAAAAAACGC 
TCTGAAAATA GTGACAAAGA CTTATCCAGG AGACGGTCCT CCAGGCTGTC CACTAATGGG 
ACCCGTGAGA TCCTAGATCC TGACTCGATT GTACCTGATC TGGTTCATAC GGTTGATACA 
AACCCTCTAC CAGACAAGTC ACCCAGTGCC AAGGATTCTG CTGAAGGTCA GTTGAAGTCT 
CCATTGGAAG CTGGCCAGGT CTCTTCTGCA TTAACTTGCC ACCCCATTGG GGATGGCCTG 
GGGGCAGCAG ATTTGGAGTT GAATTGCAAG TCAATGGGAG AAAACACGAT GAAAACAGAA 
CCTGTTTCTC CTCTTGCTGA GGTGCAGGAA GTTTCAACTG TTGAAGTTCC AAATACTTTG 
AAGAAAGTTG ATGACTCTGT GACGTTGAAT GTGCCAGCTG TGGACCTAGA CCACAAGTTT 
CGATGCAAGG TTCTGGACTG TTTGAAATTT TTCCGCAAGG CTAAATTGCT GCACTATCAT 
ATGAAGTATT TCCATGGGAT GGAGAAGTCA CCAGAGCCAG AGGAGGGCCC AGGGAAGACG 
CATGTACAGA CTCGGGGCTC TGCGGTGCCT GACAAGACAA GCCAGGAGAG CCTAACCAGG 
AAGCGGGTCT CTGCCAGTTC CCCCACTGCA AAAGAGAAGG AAAAGACTAA AGAGAAGAAA 
TTCAAAGAAC TTGTGAGAGT GAAGCCAAAG AAGAAAAAGA AAAAGAAAAA GAAAACCAAG 
CCTGAATGTC CCTGCAGTGA GGACATCAGT GATACTTCCC AGGAACCTTC TCCACCCAAA 
ACATTTGCTG TCACCAGGTG TGGGTCCTCA CACAAGCCTG GGGTCCATAT GAGCCCGCAG 
CTCCATGGTT CAGATAATGG AAACCACAAA GGGAAATTGA AAACCTGTGA GGAGGATAAT 
TTGAGTGAGT CCTCTTCCGA GAGCTTTCTT TGGAGTGATG AGGAATATGG TCAAGATGTT 
GATGTGACCA CCAACCCAGA TGAGGAGCTT GAGGGCGACG ACCGCTATGA TTTTGAGGTG 
GTCCGCTGCA TCTGTGAAGT GCAGGAGGAA AATGACTTCA TGATTCAGTG TGAAGAGTGC 
CAGTGCTGGC AGCACGGGGT CTGCATGGGC TTACTGGAAG AAAACGTGCC TGAGAAATAC 
ACCTGCTATG TTTGCCAAGA CCCTCCAGGT CAGAGGCCTG GCTTCAAGTA CTGGTATGAC 
AAGGAGTGGC TGAGCCGGGG ACACATGCAT GGTCTGGCAT TTCTGGATCA GAACTACTCC 
CACCAGAATG CCAGGAAGAT CGTGGCCACC CACCAGCTGC TCGGGGACGT GCAGAGAGTG 
ATCCAAGTGC TGCACGGCCT GCAGCTCAAG ATGAGCATTC TGCAAAGCAG AGAGCATCCT 
GATCTGCAGC TATGGTGCCA GCCCTGGAAA CAGCACTCGG GGGAGGGAAG AGCACATCCC 
AGACACATCC ACATCACAGA CGCCAGGAGC GAGGAGTCCC CAAGCTATAG AACTTTGAAT 
GGGGCGGTGG AGAAGCCATC GCCCCTGCCC CGATCTGTGG AGGAGTCTTA CATCACCAGT 
GAGCATTGCT ACCAGAAACC TCGCGCCTAT TATCCCGCTG TGGAGCAGAG GCTGGTCGTG 
GAGACGAGAG GCTCTGCCCT TGATGCTGCA GTCAGCCCCC TCTGTGAGAA CGGCGACGAC 
TCCCTCTCCC CTCGCCTAGG CTGGCCCATC GACCAAGACA GGAGCAGAGG CGACATAGAT 
CCCAAACCCA GCTCCCCAAA GGTGAGAGAG TACATTTCCA AGAATGTCTT GCCAGAAGAG 
ACGCCTGCAA GGAAGCTGCT GGACAGAGGT GGAGAAGGGC TGGTGAGCTC TCAGCACCAG 
TGGCAGTTCA ACCTGCTCAC ACATGTGGAG TCCCTGCAGG ATGAGGTGAC GCACCGGATG 
GACTCCATTG AGAAGGAGCT GGACGTGCTG GAGAGCTGGC TGGACTACAC TGGGGAGCTG 
GAGCCCCCAG AGCCACTGGC CAGGCTTCCG CAGCTCAAGC ACTGCATCAA GCAGCTGCTG 
ACTGACCTGG GCAAGGTGCA GCAGATCGCC CTCTGCTGCT CGACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phf20 ( NM_172674.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766262.2 Mus musculus PHD finger protein 20 (Phf20), mRNA.

Price & Availability↑

CloneID	OMu20847
Clone ID Related Accession (Same CDS sequence)	NM_172674.2
Accession Version	NM_172674.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	PHD finger protein 20
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ176261.1, AK045309.1, AK033017.1, AK035209.1 and BC086753.1. On or before Feb 7, 2007 this sequence version replaced XM_133187.8, XM_895086.2, XM_902654.2, XM_921093.2, XM_902647.2, XM_902658.2, XM_902645.2, XM_981080.1, XM_981121.1, XM_981157.1, XM_981001.1, XM_980964.1, XM_981043.1, XM_921032.2, XM_977098.1, NM_172674.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGACCAAGC ACCCACCTAA CAGACGAGGA ATCAGCTTTG AAGTGGGAGC CCAGTTGGAA 
GCTCGGGACC GTTTAAAAAA CTGGTATCCA GCTCATATTG AAGACATTGA CTACGAAGAA 
GGGAGAGTTC TCATCCATTT CAAGCGTTGG AACCATCGTT ACGATGAGTG GTTTTGCTGG 
GACAGTCCTT ATCTGCGCCC TTTAGAGAAG ATCCAGCTGA GGAAAGAGGG TTTACATGAT 
GAGGATGGCT CTTCTGAATT TCAAATAAAC CAGCAAGTGC TTGCTTGCTG GTCTGACTGT 
CGATTTTATC CAGCCAGAGT CACTGCTGTG AACAAGGATG GTACTTACAC TGTGAAATTT 
TATGATGGAG TAGTTCAAAC TGTCAAACAT ATTCATGTCA AAGCTTTTTC CAAAGATCAG 
AATATTGTGG GTAATGCTAG GCCTAAAGAA ACAGACCACA AAAGTCTTTC ATCGTCTCCT 
GAGAAACGAG AGAAGTTTAA AGAACAGAGA AAAGTCACGG TCAATGTGAA GAAAGACAAA 
GTGGAAAAAG CCTTAAAGAC AGAAAAGCGG CCCAAGCAAC CTGACAAAGA GGGAAAGCTG 
ATCTGCTCAG AAAAAGGCAA AGTGTCAGAG AAAAGCCTTC CTAAGAACGA AAAGGAAGAT 
AAGGAGAACA TTTCCGAGAA CGAGCGGGAG TACTCCGGGG ATGCCCAGGT GGAAAAGAAG 
CCTGAGAAGG ACCTTGTGAA GAACCCACAA GAGAACCTGA AGGAGCCAAA AAGAAAACGA 
GGCAGACCCC CTTCCATAAC TCCTACGGCT GTGGATTCAA ACTCTCAAAC TTTGCAACCA 
ATAACATTGG AATTGAGAAG ACGGAAAATA TCAAAACGAA GTGACACCCC ATTAAAGCGT 
CCCAGACTCG ACAAAAATTC ACCCCAGGAA CAGTCAAAAA AACGCTCTGA AAATAGTGAC 
AAAGACTTAT CCAGGAGACG GTCCTCCAGG CTGTCCACTA ATGGGACCCG TGAGATCCTA 
GATCCTGACT CGATTGTACC TGATCTGGTT CATACGGTTG ATACAAACCC TCTACCAGAC 
AAGTCACCCA GTGCCAAGGA TTCTGCTGAA GGTCAGTTGA AGTCTCCATT GGAAGCTGGC 
CAGGTCTCTT CTGCATTAAC TTGCCACCCC ATTGGGGATG GCCTGGGGGC AGCAGATTTG 
GAGTTGAATT GCAAGTCAAT GGGAGAAAAC ACGATGAAAA CAGAACCTGT TTCTCCTCTT 
GCTGAGGTGC AGGAAGTTTC AACTGTTGAA GTTCCAAATA CTTTGAAGAA AGTTGATGAC 
TCTGTGACGT TGAATGTGCC AGCTGTGGAC CTAGACCACA AGTTTCGATG CAAGGTTCTG 
GACTGTTTGA AATTTTTCCG CAAGGCTAAA TTGCTGCACT ATCATATGAA GTATTTCCAT 
GGGATGGAGA AGTCACCAGA GCCAGAGGAG GGCCCAGGGA AGACGCATGT ACAGACTCGG 
GGCTCTGCGG TGCCTGACAA GACAAGCCAG GAGAGCCTAA CCAGGAAGCG GGTCTCTGCC 
AGTTCCCCCA CTGCAAAAGA GAAGGAAAAG ACTAAAGAGA AGAAATTCAA AGAACTTGTG 
AGAGTGAAGC CAAAGAAGAA AAAGAAAAAG AAAAAGAAAA CCAAGCCTGA ATGTCCCTGC 
AGTGAGGACA TCAGTGATAC TTCCCAGGAA CCTTCTCCAC CCAAAACATT TGCTGTCACC 
AGGTGTGGGT CCTCACACAA GCCTGGGGTC CATATGAGCC CGCAGCTCCA TGGTTCAGAT 
AATGGAAACC ACAAAGGGAA ATTGAAAACC TGTGAGGAGG ATAATTTGAG TGAGTCCTCT 
TCCGAGAGCT TTCTTTGGAG TGATGAGGAA TATGGTCAAG ATGTTGATGT GACCACCAAC 
CCAGATGAGG AGCTTGAGGG CGACGACCGC TATGATTTTG AGGTGGTCCG CTGCATCTGT 
GAAGTGCAGG AGGAAAATGA CTTCATGATT CAGTGTGAAG AGTGCCAGTG CTGGCAGCAC 
GGGGTCTGCA TGGGCTTACT GGAAGAAAAC GTGCCTGAGA AATACACCTG CTATGTTTGC 
CAAGACCCTC CAGGTCAGAG GCCTGGCTTC AAGTACTGGT ATGACAAGGA GTGGCTGAGC 
CGGGGACACA TGCATGGTCT GGCATTTCTG GATCAGAACT ACTCCCACCA GAATGCCAGG 
AAGATCGTGG CCACCCACCA GCTGCTCGGG GACGTGCAGA GAGTGATCCA AGTGCTGCAC 
GGCCTGCAGC TCAAGATGAG CATTCTGCAA AGCAGAGAGC ATCCTGATCT GCAGCTATGG 
TGCCAGCCCT GGAAACAGCA CTCGGGGGAG GGAAGAGCAC ATCCCAGACA CATCCACATC 
ACAGACGCCA GGAGCGAGGA GTCCCCAAGC TATAGAACTT TGAATGGGGC GGTGGAGAAG 
CCATCGCCCC TGCCCCGATC TGTGGAGGAG TCTTACATCA CCAGTGAGCA TTGCTACCAG 
AAACCTCGCG CCTATTATCC CGCTGTGGAG CAGAGGCTGG TCGTGGAGAC GAGAGGCTCT 
GCCCTTGATG CTGCAGTCAG CCCCCTCTGT GAGAACGGCG ACGACTCCCT CTCCCCTCGC 
CTAGGCTGGC CCATCGACCA AGACAGGAGC AGAGGCGACA TAGATCCCAA ACCCAGCTCC 
CCAAAGGTGA GAGAGTACAT TTCCAAGAAT GTCTTGCCAG AAGAGACGCC TGCAAGGAAG 
CTGCTGGACA GAGGTGGAGA AGGGCTGGTG AGCTCTCAGC ACCAGTGGCA GTTCAACCTG 
CTCACACATG TGGAGTCCCT GCAGGATGAG GTGACGCACC GGATGGACTC CATTGAGAAG 
GAGCTGGACG TGCTGGAGAG CTGGCTGGAC TACACTGGGG AGCTGGAGCC CCCAGAGCCA 
CTGGCCAGGC TTCCGCAGCT CAAGCACTGC ATCAAGCAGC TGCTGACTGA CCTGGGCAAG 
GTGCAGCAGA TCGCCCTCTG CTGCTCGACA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766262.2
CDS	184..3216
Translation	MTKHPPNRRGISFEVGAQLEARDRLKNWYPAHIEDIDYEEGRVLIHFKRWNHRYDEWFCWDSPYLRPLEKIQLRKEGLHDEDGSSEFQINQQVLACWSDCRFYPARVTAVNKDGTYTVKFYDGVVQTVKHIHVKAFSKDQNIVGNARPKETDHKSLSSSPEKREKFKEQRKVTVNVKKDKVEKALKTEKRPKQPDKEGKLICSEKGKVSEKSLPKNEKEDKENISENEREYSGDAQVEKKPEKDLVKNPQENLKEPKRKRGRPPSITPTAVDSNSQTLQPITLELRRRKISKRSDTPLKRPRLDKNSPQEQSKKRSENSDKDLSRRRSSRLSTNGTREILDPDSIVPDLVHTVDTNPLPDKSPSAKDSAEGQLKSPLEAGQVSSALTCHPIGDGLGAADLELNCKSMGENTMKTEPVSPLAEVQEVSTVEVPNTLKKVDDSVTLNVPAVDLDHKFRCKVLDCLKFFRKAKLLHYHMKYFHGMEKSPEPEEGPGKTHVQTRGSAVPDKTSQESLTRKRVSASSPTAKEKEKTKEKKFKELVRVKPKKKKKKKKKTKPECPCSEDISDTSQEPSPPKTFAVTRCGSSHKPGVHMSPQLHGSDNGNHKGKLKTCEEDNLSESSSESFLWSDEEYGQDVDVTTNPDEELEGDDRYDFEVVRCICEVQEENDFMIQCEECQCWQHGVCMGLLEENVPEKYTCYVCQDPPGQRPGFKYWYDKEWLSRGHMHGLAFLDQNYSHQNARKIVATHQLLGDVQRVIQVLHGLQLKMSILQSREHPDLQLWCQPWKQHSGEGRAHPRHIHITDARSEESPSYRTLNGAVEKPSPLPRSVEESYITSEHCYQKPRAYYPAVEQRLVVETRGSALDAAVSPLCENGDDSLSPRLGWPIDQDRSRGDIDPKPSSPKVREYISKNVLPEETPARKLLDRGGEGLVSSQHQWQFNLLTHVESLQDEVTHRMDSIEKELDVLESWLDYTGELEPPEPLARLPQLKHCIKQLLTDLGKVQQIALCCST

Target ORF information:

RefSeq Version	NM_172674.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus PHD finger protein 20 (Phf20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172674.2

ORF Insert Sequence:

ATGACCAAGC ACCCACCTAA CAGACGAGGA ATCAGCTTTG AAGTGGGAGC CCAGTTGGAA 
GCTCGGGACC GTTTAAAAAA CTGGTATCCA GCTCATATTG AAGACATTGA CTACGAAGAA 
GGGAGAGTTC TCATCCATTT CAAGCGTTGG AACCATCGTT ACGATGAGTG GTTTTGCTGG 
GACAGTCCTT ATCTGCGCCC TTTAGAGAAG ATCCAGCTGA GGAAAGAGGG TTTACATGAT 
GAGGATGGCT CTTCTGAATT TCAAATAAAC CAGCAAGTGC TTGCTTGCTG GTCTGACTGT 
CGATTTTATC CAGCCAGAGT CACTGCTGTG AACAAGGATG GTACTTACAC TGTGAAATTT 
TATGATGGAG TAGTTCAAAC TGTCAAACAT ATTCATGTCA AAGCTTTTTC CAAAGATCAG 
AATATTGTGG GTAATGCTAG GCCTAAAGAA ACAGACCACA AAAGTCTTTC ATCGTCTCCT 
GAGAAACGAG AGAAGTTTAA AGAACAGAGA AAAGTCACGG TCAATGTGAA GAAAGACAAA 
GTGGAAAAAG CCTTAAAGAC AGAAAAGCGG CCCAAGCAAC CTGACAAAGA GGGAAAGCTG 
ATCTGCTCAG AAAAAGGCAA AGTGTCAGAG AAAAGCCTTC CTAAGAACGA AAAGGAAGAT 
AAGGAGAACA TTTCCGAGAA CGAGCGGGAG TACTCCGGGG ATGCCCAGGT GGAAAAGAAG 
CCTGAGAAGG ACCTTGTGAA GAACCCACAA GAGAACCTGA AGGAGCCAAA AAGAAAACGA 
GGCAGACCCC CTTCCATAAC TCCTACGGCT GTGGATTCAA ACTCTCAAAC TTTGCAACCA 
ATAACATTGG AATTGAGAAG ACGGAAAATA TCAAAACGAA GTGACACCCC ATTAAAGCGT 
CCCAGACTCG ACAAAAATTC ACCCCAGGAA CAGTCAAAAA AACGCTCTGA AAATAGTGAC 
AAAGACTTAT CCAGGAGACG GTCCTCCAGG CTGTCCACTA ATGGGACCCG TGAGATCCTA 
GATCCTGACT CGATTGTACC TGATCTGGTT CATACGGTTG ATACAAACCC TCTACCAGAC 
AAGTCACCCA GTGCCAAGGA TTCTGCTGAA GGTCAGTTGA AGTCTCCATT GGAAGCTGGC 
CAGGTCTCTT CTGCATTAAC TTGCCACCCC ATTGGGGATG GCCTGGGGGC AGCAGATTTG 
GAGTTGAATT GCAAGTCAAT GGGAGAAAAC ACGATGAAAA CAGAACCTGT TTCTCCTCTT 
GCTGAGGTGC AGGAAGTTTC AACTGTTGAA GTTCCAAATA CTTTGAAGAA AGTTGATGAC 
TCTGTGACGT TGAATGTGCC AGCTGTGGAC CTAGACCACA AGTTTCGATG CAAGGTTCTG 
GACTGTTTGA AATTTTTCCG CAAGGCTAAA TTGCTGCACT ATCATATGAA GTATTTCCAT 
GGGATGGAGA AGTCACCAGA GCCAGAGGAG GGCCCAGGGA AGACGCATGT ACAGACTCGG 
GGCTCTGCGG TGCCTGACAA GACAAGCCAG GAGAGCCTAA CCAGGAAGCG GGTCTCTGCC 
AGTTCCCCCA CTGCAAAAGA GAAGGAAAAG ACTAAAGAGA AGAAATTCAA AGAACTTGTG 
AGAGTGAAGC CAAAGAAGAA AAAGAAAAAG AAAAAGAAAA CCAAGCCTGA ATGTCCCTGC 
AGTGAGGACA TCAGTGATAC TTCCCAGGAA CCTTCTCCAC CCAAAACATT TGCTGTCACC 
AGGTGTGGGT CCTCACACAA GCCTGGGGTC CATATGAGCC CGCAGCTCCA TGGTTCAGAT 
AATGGAAACC ACAAAGGGAA ATTGAAAACC TGTGAGGAGG ATAATTTGAG TGAGTCCTCT 
TCCGAGAGCT TTCTTTGGAG TGATGAGGAA TATGGTCAAG ATGTTGATGT GACCACCAAC 
CCAGATGAGG AGCTTGAGGG CGACGACCGC TATGATTTTG AGGTGGTCCG CTGCATCTGT 
GAAGTGCAGG AGGAAAATGA CTTCATGATT CAGTGTGAAG AGTGCCAGTG CTGGCAGCAC 
GGGGTCTGCA TGGGCTTACT GGAAGAAAAC GTGCCTGAGA AATACACCTG CTATGTTTGC 
CAAGACCCTC CAGGTCAGAG GCCTGGCTTC AAGTACTGGT ATGACAAGGA GTGGCTGAGC 
CGGGGACACA TGCATGGTCT GGCATTTCTG GATCAGAACT ACTCCCACCA GAATGCCAGG 
AAGATCGTGG CCACCCACCA GCTGCTCGGG GACGTGCAGA GAGTGATCCA AGTGCTGCAC 
GGCCTGCAGC TCAAGATGAG CATTCTGCAA AGCAGAGAGC ATCCTGATCT GCAGCTATGG 
TGCCAGCCCT GGAAACAGCA CTCGGGGGAG GGAAGAGCAC ATCCCAGACA CATCCACATC 
ACAGACGCCA GGAGCGAGGA GTCCCCAAGC TATAGAACTT TGAATGGGGC GGTGGAGAAG 
CCATCGCCCC TGCCCCGATC TGTGGAGGAG TCTTACATCA CCAGTGAGCA TTGCTACCAG 
AAACCTCGCG CCTATTATCC CGCTGTGGAG CAGAGGCTGG TCGTGGAGAC GAGAGGCTCT 
GCCCTTGATG CTGCAGTCAG CCCCCTCTGT GAGAACGGCG ACGACTCCCT CTCCCCTCGC 
CTAGGCTGGC CCATCGACCA AGACAGGAGC AGAGGCGACA TAGATCCCAA ACCCAGCTCC 
CCAAAGGTGA GAGAGTACAT TTCCAAGAAT GTCTTGCCAG AAGAGACGCC TGCAAGGAAG 
CTGCTGGACA GAGGTGGAGA AGGGCTGGTG AGCTCTCAGC ACCAGTGGCA GTTCAACCTG 
CTCACACATG TGGAGTCCCT GCAGGATGAG GTGACGCACC GGATGGACTC CATTGAGAAG 
GAGCTGGACG TGCTGGAGAG CTGGCTGGAC TACACTGGGG AGCTGGAGCC CCCAGAGCCA 
CTGGCCAGGC TTCCGCAGCT CAAGCACTGC ATCAAGCAGC TGCTGACTGA CCTGGGCAAG 
GTGCAGCAGA TCGCCCTCTG CTGCTCGACA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An efficient method for generation of bi-allelic null mutant mouse embryonic stem cells and its application for investigating epigenetic modifiers.
Nucleic acids research45(21)e174(2017 Dec)
Fisher CL,Marks H,Cho LT,Andrews R,Wormald S,Carroll T,Iyer V,Tate P,Rosen B,Stunnenberg HG,Fisher AG,Skarnes WC

PHF20 positively regulates osteoblast differentiation via increasing the expression and activation of Runx2 with enrichment of H3K4me3.
Scientific reports7(1)8060(2017 Aug)
Yang JW,Jeong BC,Park J,Koh JT

Jmjd3 inhibits reprogramming by upregulating expression of INK4a/Arf and targeting PHF20 for ubiquitination.
Cell152(5)1037-50(2013 Feb)
Zhao W,Li Q,Ayers S,Gu Y,Shi Z,Zhu Q,Chen Y,Wang HY,Wang RF

Loss of the methyl lysine effector protein PHF20 impacts the expression of genes regulated by the lysine acetyltransferase MOF.
The Journal of biological chemistry287(1)429-37(2012 Jan)
Badeaux AI,Yang Y,Cardenas K,Vemulapalli V,Chen K,Kusewitt D,Richie E,Li W,Bedford MT

Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst.
Developmental cell18(4)675-85(2010 Apr)
Guo G,Huss M,Tong GQ,Wang C,Li Sun L,Clarke ND,Robson P

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Phf20 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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