Erfe cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Erfe
Entrez Gene ID	227358
Full Name	erythroferrone
Synonyms	4832406C22,Fam132b,myonectin
General protein information	Preferred Names erythroferrone Names erythroferrone complement C1q tumor necrosis factor-related protein 15 family with sequence similarity 132, member B protein FAM132B
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 D

mRNA and Protein(s)

mRNA	Protein	Name
XM_006529485.3	XP_006529548.1	erythroferrone isoform X2
XM_006529485.4	XP_006529548.1	erythroferrone isoform X2
XM_006529487.3	XP_006529550.1	erythroferrone isoform X3
XM_030253764.1	XP_030109624.1	erythroferrone isoform X2
XM_006529484.3	XP_006529547.1	erythroferrone isoform X1
XM_006529484.4	XP_006529547.1	erythroferrone isoform X1
NM_173395.2	NP_775571.2	erythroferrone precursor
XM_006529487.4	XP_006529550.1	erythroferrone isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FAM132B	XP_003950054.1
Homo sapiens (human)	FAM132B	XP_006712968.1
	LOC706226	XP_001094581.2
Bos taurus (cattle)	FAM132B	XP_005205094.1
Rattus norvegicus (Norway rat)	Fam132b	XP_001060107.2
Mus musculus (house mouse)	Fam132b	NP_775571.2

Gene Ontology
Bibliography

Related articles in PubMed

Hepcidin is regulated by promoter-associated histone acetylation and HDAC3.
Pasricha SR, Lim PJ, Duarte TL, Casu C, Oosterhuis D, Mleczko-Sanecka K, Suciu M, Da Silva AR, Al-Hourani K, Arezes J, McHugh K, Gooding S, Frost JN, Wray K, Santos A, Porto G, Repapi E, Gray N, Draper SJ, Ashley N, Soilleux E, Olinga P, Muckenthaler MU, Hughes JR, Rivella S, Milne TA, Armitage AE, Drakesmith H
Nature communications8(1)403(2017 09)

Effect of erythropoietin administration on proteins participating in iron homeostasis in Tmprss6-mutated mask mice.
Fr?dlov? J, Rychtar??kov? Z, Gurieva I, Vokurka M, Truksa J, Krijt J
PloS one12(10)e0186844(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Erythroferrone and matriptase-2 independently regulate hepcidin expression.
Title: Erythroferrone and matriptase-2 independently regulate hepcidin expression.

The lack of effect of erythropoietin on hepcidin expression in mask mice can not be explained by changes in erythroferrone synthesis, as splenic erythroferrone content increased after erythropoietin administration in both C57BL/6 and mask mice.
Title: Effect of erythropoietin administration on proteins participating in iron homeostasis in Tmprss6-mutated mask mice.

Erfe(-/-) mice failed to adequately suppress hepcidin expression after infection with Plasmodium berghei K173. Importantly, the sustained production of hepcidin allowed by erythroferrone ablation was associated with decreased parasitemia, providing further evidence that transient iron restriction could be beneficial in the treatment of malaria.
Title: Erythroferrone contributes to hepcidin repression in a mouse model of malarial anemia.

The here presented data unearthed EPO-dependent erythroferrone expression acts as an erythropoiesis-driven regulator of iron metabolism under phenylhydrazine-induced hemolytic anemia.
Title: EPO-dependent induction of erythroferrone drives hepcidin suppression and systematic iron absorption under phenylhydrazine-induced hemolytic anemia.

Erythroferrone contributes to hepcidin suppression and iron overload in a mouse model of beta-thalassemia.
Title: Erythroferrone contributes to hepcidin suppression and iron overload in a mouse model of ?-thalassemia.

The following Erfe gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Erfe cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18621	NM_173395.2 Latest version!	Mus musculus erythroferrone (Erfe), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OMu28164	XM_006529484.4 Latest version!	Mus musculus erythroferrone (Erfe), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu28165	XM_030253764.1 Latest version!	Mus musculus erythroferrone (Erfe), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu28165	XM_006529485.4 Latest version!	Mus musculus erythroferrone (Erfe), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu28166	XM_006529487.4 Latest version!	Mus musculus erythroferrone (Erfe), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu28165	XM_006529485.3	Mus musculus family with sequence similarity 132, member B (Fam132b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
OMu28164	XM_006529484.3	Mus musculus family with sequence similarity 132, member B (Fam132b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
OMu28166	XM_006529487.3	Mus musculus family with sequence similarity 132, member B (Fam132b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Erfe ( NM_173395.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_775571.2 Mus musculus erythroferrone (Erfe), mRNA.

Price & Availability↑

CloneID	OMu18621
Clone ID Related Accession (Same CDS sequence)	NM_173395.2
Accession Version	NM_173395.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Mus musculus(house mouse)
Product	erythroferrone precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC056923.1. On Jan 27, 2007 this sequence version replaced NM_173395.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC056923.1, AK029264.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849377 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTCGA CCCGCCGCCC CGTCGGAGCT CGCACGCTGC TCGCCTGCGC CAGCCTACTC 
GCCGCCATGG GCCTCGGTGT CCCTGAGTCC GCGGAGCCCG TGGGGACTCA TGCACGCCCG 
CAGCCGCCCG GGGCCGAGCT GCCCGCCCCG CCAGCCAACA GCCCGCCGGA ACCCACCATT 
GCGCATGCAC ACAGTGTGGA TCCCCGGGAT GCTTGGATGC TGTTCGTCAA GCAGAGTGAC 
AAGGGGATCA ACAGTAAGAG GAGGAGCAAA GCCAGGAGGC TGAAGCTTGG CCTGCCAGGA 
CCCCCAGGGC CACCAGGTCC TCAGGGCCCC CCAGGCCCCT TTATCCCATC TGAGGTTCTG 
CTGAAGGAGT TCCAGCTGTT GCTGAAAGGC GCAGTACGGC AGCGAGAGAG CCATCTGGAG 
CACTGCACCA GGGATCTCAC TACACCAGCC TCGGGTAGCC CTTCCCGTGT CCCAGCCGCC 
CAGGAGCTTG ATAGCCAGGA CCCAGGGGCA TTGTTAGCTC TGCTGGCTGC GACCTTGGCC 
CAGGGCCCGC GGGCACCACG TGTGGAGGCC GCATTCCACT GTCGCTTGCG CCGGGATGTG 
CAGGTGGATC GGCGTGCGTT GCACGAGCTT GGGATCTACT ACCTGCCCGA AGTTGAGGGA 
GCCTTCCACC GGGGCCCAGG CTTGAATCTG ACCAGCGGCC AGTACACCGC ACCTGTGGCT 
GGCTTCTATG CGCTTGCTGC CACTCTGCAC GTGGCACTCA CCGAGCAGCC AAGAAAGGGA 
CCAACACGAC CCCGGGATCG TCTGCGCCTG CTGATCTGCA TCCAGTCTCT CTGCCAGCAC 
AATGCCTCCC TGGAGACTGT GATGGGGCTG GAGAACAGCA GCGAGCTCTT CACCATCTCA 
GTAAATGGTG TCCTCTATCT ACAGGCAGGA CACTACACTT CTGTCTTCTT GGACAATGCC 
AGCGGCTCCT CCCTCACGGT ACGCAGTGGC TCTCACTTCA GTGCTATCCT CCTGGGCCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775571.2
CDS	52..1074
Translation	MASTRRPVGARTLLACASLLAAMGLGVPESAEPVGTHARPQPPGAELPAPPANSPPEPTIAHAHSVDPRDAWMLFVKQSDKGINSKRRSKARRLKLGLPGPPGPPGPQGPPGPFIPSEVLLKEFQLLLKGAVRQRESHLEHCTRDLTTPASGSPSRVPAAQELDSQDPGALLALLAATLAQGPRAPRVEAAFHCRLRRDVQVDRRALHELGIYYLPEVEGAFHRGPGLNLTSGQYTAPVAGFYALAATLHVALTEQPRKGPTRPRDRLRLLICIQSLCQHNASLETVMGLENSSELFTISVNGVLYLQAGHYTSVFLDNASGSSLTVRSGSHFSAILLGL

Target ORF information:

RefSeq Version	NM_173395.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus erythroferrone (Erfe), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173395.2

ORF Insert Sequence:

ATGGCCTCGA CCCGCCGCCC CGTCGGAGCT CGCACGCTGC TCGCCTGCGC CAGCCTACTC 
GCCGCCATGG GCCTCGGTGT CCCTGAGTCC GCGGAGCCCG TGGGGACTCA TGCACGCCCG 
CAGCCGCCCG GGGCCGAGCT GCCCGCCCCG CCAGCCAACA GCCCGCCGGA ACCCACCATT 
GCGCATGCAC ACAGTGTGGA TCCCCGGGAT GCTTGGATGC TGTTCGTCAA GCAGAGTGAC 
AAGGGGATCA ACAGTAAGAG GAGGAGCAAA GCCAGGAGGC TGAAGCTTGG CCTGCCAGGA 
CCCCCAGGGC CACCAGGTCC TCAGGGCCCC CCAGGCCCCT TTATCCCATC TGAGGTTCTG 
CTGAAGGAGT TCCAGCTGTT GCTGAAAGGC GCAGTACGGC AGCGAGAGAG CCATCTGGAG 
CACTGCACCA GGGATCTCAC TACACCAGCC TCGGGTAGCC CTTCCCGTGT CCCAGCCGCC 
CAGGAGCTTG ATAGCCAGGA CCCAGGGGCA TTGTTAGCTC TGCTGGCTGC GACCTTGGCC 
CAGGGCCCGC GGGCACCACG TGTGGAGGCC GCATTCCACT GTCGCTTGCG CCGGGATGTG 
CAGGTGGATC GGCGTGCGTT GCACGAGCTT GGGATCTACT ACCTGCCCGA AGTTGAGGGA 
GCCTTCCACC GGGGCCCAGG CTTGAATCTG ACCAGCGGCC AGTACACCGC ACCTGTGGCT 
GGCTTCTATG CGCTTGCTGC CACTCTGCAC GTGGCACTCA CCGAGCAGCC AAGAAAGGGA 
CCAACACGAC CCCGGGATCG TCTGCGCCTG CTGATCTGCA TCCAGTCTCT CTGCCAGCAC 
AATGCCTCCC TGGAGACTGT GATGGGGCTG GAGAACAGCA GCGAGCTCTT CACCATCTCA 
GTAAATGGTG TCCTCTATCT ACAGGCAGGA CACTACACTT CTGTCTTCTT GGACAATGCC 
AGCGGCTCCT CCCTCACGGT ACGCAGTGGC TCTCACTTCA GTGCTATCCT CCTGGGCCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Erfe ( XM_006529484.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529547.1 Mus musculus erythroferrone (Erfe), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu28164
Clone ID Related Accession (Same CDS sequence)	XM_006529484.3 , XM_006529484.4
Accession Version	XM_006529484.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	erythroferrone isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529484.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGA CCCGCCGCCC CGTCGGAGCT CGCACGCTGC TCGCCTGCGC CAGCCTACTC 
GCCGCCATGG GCCTCGGTGT CCCTGAGTCC GCGGAGCCCG TGGGGACTCA TGCACGCCCG 
CAGCCGCCCG GGGCCGAGCT GCCCGCCCCG CCAGCCAACA GCCCGCCGGA ACCCACCATT 
GCGCATGCAC ACAGTGTGGA TCCCCGGGAT GCTTGGATGC TGTTCGTCAA GCAGAGTGAC 
AAGGGGATCA ACAGTAAGAG GAGGAGCAAA GCCAGGAGGC TGAAGCTTGG CCTGCCAGGA 
CCCCCAGGGC CACCAGGTCC TCAGGGCCCC CCAGGCCCCT TTATCCCATC TGAGGTTCTG 
CTGAAGGAGT TCCAGCTGTT GCTGAAAGGC GCAGTACGGC AGCGAGAGAG CCATCTGGAG 
CACTGCACCA GGGATCTCAC TACACCAGCC TCGGGTAGCC CTTCCCGTGT CCCAGCCGCC 
CAGGAGCTTG ATAGCCAGGA CCCAGGGGCA TTGTTAGCTC TGCTGGCTGC GACCTTGGCC 
CAGGGCCCGC GGGCACCACG TGTGGAGGCC GCATTCCACT GTCGCTTGCG CCGGGATGTG 
CAGGTGGATC GGCGTGCGTT GCACGAGCTT GGGATCTACT ACCTGCCCGA AGTTGAGGGA 
GCCTTCCACC GGGGCCCAGG CTTGAATCTG ACCAGCGGCC AGTACACCGC ACCTGTGGCT 
GGCTTCTATG CGCTTGCTGC CACTCTGCAC GTGGCACTCA CCGAGCAGCC AAGAAAGGGA 
CCAACACGAC CCCGGGATCG TCTGCGCCTG CTGATCTGCA TCCAGTCTCT CTGCCAGCAC 
AATGCCTCCC TGGAGACTGT GATGGGGCTG GAGAACAGCA GCGAGCTCTT CACCATCTCA 
GTAAATGGTG TCCTCTATCT ACAGGCAGGA CACTACACTT CTGTCTTCTT GGACAATGCC 
AGCGGCTCCT CCCTCACGCT GACACCAGGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529547.1
CDS	143..1135
Translation	MASTRRPVGARTLLACASLLAAMGLGVPESAEPVGTHARPQPPGAELPAPPANSPPEPTIAHAHSVDPRDAWMLFVKQSDKGINSKRRSKARRLKLGLPGPPGPPGPQGPPGPFIPSEVLLKEFQLLLKGAVRQRESHLEHCTRDLTTPASGSPSRVPAAQELDSQDPGALLALLAATLAQGPRAPRVEAAFHCRLRRDVQVDRRALHELGIYYLPEVEGAFHRGPGLNLTSGQYTAPVAGFYALAATLHVALTEQPRKGPTRPRDRLRLLICIQSLCQHNASLETVMGLENSSELFTISVNGVLYLQAGHYTSVFLDNASGSSLTLTPG

Target ORF information:

RefSeq Version	XM_006529484.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus erythroferrone (Erfe), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529484.4

ORF Insert Sequence:

ATGGCCTCGA CCCGCCGCCC CGTCGGAGCT CGCACGCTGC TCGCCTGCGC CAGCCTACTC 
GCCGCCATGG GCCTCGGTGT CCCTGAGTCC GCGGAGCCCG TGGGGACTCA TGCACGCCCG 
CAGCCGCCCG GGGCCGAGCT GCCCGCCCCG CCAGCCAACA GCCCGCCGGA ACCCACCATT 
GCGCATGCAC ACAGTGTGGA TCCCCGGGAT GCTTGGATGC TGTTCGTCAA GCAGAGTGAC 
AAGGGGATCA ACAGTAAGAG GAGGAGCAAA GCCAGGAGGC TGAAGCTTGG CCTGCCAGGA 
CCCCCAGGGC CACCAGGTCC TCAGGGCCCC CCAGGCCCCT TTATCCCATC TGAGGTTCTG 
CTGAAGGAGT TCCAGCTGTT GCTGAAAGGC GCAGTACGGC AGCGAGAGAG CCATCTGGAG 
CACTGCACCA GGGATCTCAC TACACCAGCC TCGGGTAGCC CTTCCCGTGT CCCAGCCGCC 
CAGGAGCTTG ATAGCCAGGA CCCAGGGGCA TTGTTAGCTC TGCTGGCTGC GACCTTGGCC 
CAGGGCCCGC GGGCACCACG TGTGGAGGCC GCATTCCACT GTCGCTTGCG CCGGGATGTG 
CAGGTGGATC GGCGTGCGTT GCACGAGCTT GGGATCTACT ACCTGCCCGA AGTTGAGGGA 
GCCTTCCACC GGGGCCCAGG CTTGAATCTG ACCAGCGGCC AGTACACCGC ACCTGTGGCT 
GGCTTCTATG CGCTTGCTGC CACTCTGCAC GTGGCACTCA CCGAGCAGCC AAGAAAGGGA 
CCAACACGAC CCCGGGATCG TCTGCGCCTG CTGATCTGCA TCCAGTCTCT CTGCCAGCAC 
AATGCCTCCC TGGAGACTGT GATGGGGCTG GAGAACAGCA GCGAGCTCTT CACCATCTCA 
GTAAATGGTG TCCTCTATCT ACAGGCAGGA CACTACACTT CTGTCTTCTT GGACAATGCC 
AGCGGCTCCT CCCTCACGCT GACACCAGGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Erfe ( XM_006529485.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529548.1 Mus musculus erythroferrone (Erfe), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu28165
Clone ID Related Accession (Same CDS sequence)	XM_006529485.3 , XM_006529485.4 , XM_030253764.1
Accession Version	XM_006529485.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	erythroferrone isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529485.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGA CCCGCCGCCC CGTCGGAGCT CGCACGCTGC TCGCCTGCGC CAGCCTACTC 
GCCGCCATGG GCCTCGGTGT CCCTGAGTCC GCGGAGCCCG TGGGGACTCA TGCACGCCCG 
CAGCCGCCCG GGGCCGAGCT GCCCGCCCCG CCAGCCAACA GCCCGCCGGA ACCCACCATT 
GCGCATGCAC ACAGTGTGGA TCCCCGGGAT GCTTGGATGC TGTTCGTCAA GCAGAGTGAC 
AAGGGGATCA ACAGTAAGAG GAGGAGCAAA GCCAGGAGGC TGAAGCTTGG CCTGCCAGGA 
CCCCCAGGGC CACCAGGTCC TCAGGGCCCC CCAGGCCCCT TTATCCCATC TGAGGTTCTG 
CTGAAGGAGT TCCAGCTGTT GCTGAAAGGC GCAGTACGGC AGCGAGAGAG CCATCTGGAG 
CACTGCACCA GGGATCTCAC TACACCAGCC TCGGGTAGCC CTTCCCGTGT CCCAGCCGCC 
CAGGAGCTTG ATAGCCAGGA CCCAGGGGCA TTGTTAGCTC TGCTGGCTGC GACCTTGGCC 
CAGGGCCCGC GGGCACCACG TGTGGAGGCC GCATTCCACT GTCGCTTGCG CCGGGATGTG 
CAGGTGGATC GGCGTGCGTT GCACGAGCTT GGGATCTACT ACCTGCCCGA AGTTGAGGGA 
GCCTTCCACC GGGGCCCAGG CTTGAATCTG ACCAGCGGCC AGTACACCGC ACCTGTGGCT 
GGCTTCTATG CGCTTGCTGC CACTCTGCAC CACTCACCGA GCAGCCAAGA AAGGGACCAA 
CACGACCCCG GGATCGTCTG CGCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529548.1
CDS	169..978
Translation	MASTRRPVGARTLLACASLLAAMGLGVPESAEPVGTHARPQPPGAELPAPPANSPPEPTIAHAHSVDPRDAWMLFVKQSDKGINSKRRSKARRLKLGLPGPPGPPGPQGPPGPFIPSEVLLKEFQLLLKGAVRQRESHLEHCTRDLTTPASGSPSRVPAAQELDSQDPGALLALLAATLAQGPRAPRVEAAFHCRLRRDVQVDRRALHELGIYYLPEVEGAFHRGPGLNLTSGQYTAPVAGFYALAATLHHSPSSQERDQHDPGIVCAC

Target ORF information:

RefSeq Version	XM_006529485.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus erythroferrone (Erfe), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529485.4

ORF Insert Sequence:

ATGGCCTCGA CCCGCCGCCC CGTCGGAGCT CGCACGCTGC TCGCCTGCGC CAGCCTACTC 
GCCGCCATGG GCCTCGGTGT CCCTGAGTCC GCGGAGCCCG TGGGGACTCA TGCACGCCCG 
CAGCCGCCCG GGGCCGAGCT GCCCGCCCCG CCAGCCAACA GCCCGCCGGA ACCCACCATT 
GCGCATGCAC ACAGTGTGGA TCCCCGGGAT GCTTGGATGC TGTTCGTCAA GCAGAGTGAC 
AAGGGGATCA ACAGTAAGAG GAGGAGCAAA GCCAGGAGGC TGAAGCTTGG CCTGCCAGGA 
CCCCCAGGGC CACCAGGTCC TCAGGGCCCC CCAGGCCCCT TTATCCCATC TGAGGTTCTG 
CTGAAGGAGT TCCAGCTGTT GCTGAAAGGC GCAGTACGGC AGCGAGAGAG CCATCTGGAG 
CACTGCACCA GGGATCTCAC TACACCAGCC TCGGGTAGCC CTTCCCGTGT CCCAGCCGCC 
CAGGAGCTTG ATAGCCAGGA CCCAGGGGCA TTGTTAGCTC TGCTGGCTGC GACCTTGGCC 
CAGGGCCCGC GGGCACCACG TGTGGAGGCC GCATTCCACT GTCGCTTGCG CCGGGATGTG 
CAGGTGGATC GGCGTGCGTT GCACGAGCTT GGGATCTACT ACCTGCCCGA AGTTGAGGGA 
GCCTTCCACC GGGGCCCAGG CTTGAATCTG ACCAGCGGCC AGTACACCGC ACCTGTGGCT 
GGCTTCTATG CGCTTGCTGC CACTCTGCAC CACTCACCGA GCAGCCAAGA AAGGGACCAA 
CACGACCCCG GGATCGTCTG CGCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Erfe ( XM_030253764.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109624.1 Mus musculus erythroferrone (Erfe), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu28165
Clone ID Related Accession (Same CDS sequence)	XM_006529485.3 , XM_006529485.4 , XM_030253764.1
Accession Version	XM_030253764.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	erythroferrone isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGA CCCGCCGCCC CGTCGGAGCT CGCACGCTGC TCGCCTGCGC CAGCCTACTC 
GCCGCCATGG GCCTCGGTGT CCCTGAGTCC GCGGAGCCCG TGGGGACTCA TGCACGCCCG 
CAGCCGCCCG GGGCCGAGCT GCCCGCCCCG CCAGCCAACA GCCCGCCGGA ACCCACCATT 
GCGCATGCAC ACAGTGTGGA TCCCCGGGAT GCTTGGATGC TGTTCGTCAA GCAGAGTGAC 
AAGGGGATCA ACAGTAAGAG GAGGAGCAAA GCCAGGAGGC TGAAGCTTGG CCTGCCAGGA 
CCCCCAGGGC CACCAGGTCC TCAGGGCCCC CCAGGCCCCT TTATCCCATC TGAGGTTCTG 
CTGAAGGAGT TCCAGCTGTT GCTGAAAGGC GCAGTACGGC AGCGAGAGAG CCATCTGGAG 
CACTGCACCA GGGATCTCAC TACACCAGCC TCGGGTAGCC CTTCCCGTGT CCCAGCCGCC 
CAGGAGCTTG ATAGCCAGGA CCCAGGGGCA TTGTTAGCTC TGCTGGCTGC GACCTTGGCC 
CAGGGCCCGC GGGCACCACG TGTGGAGGCC GCATTCCACT GTCGCTTGCG CCGGGATGTG 
CAGGTGGATC GGCGTGCGTT GCACGAGCTT GGGATCTACT ACCTGCCCGA AGTTGAGGGA 
GCCTTCCACC GGGGCCCAGG CTTGAATCTG ACCAGCGGCC AGTACACCGC ACCTGTGGCT 
GGCTTCTATG CGCTTGCTGC CACTCTGCAC CACTCACCGA GCAGCCAAGA AAGGGACCAA 
CACGACCCCG GGATCGTCTG CGCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109624.1
CDS	172..981
Translation	MASTRRPVGARTLLACASLLAAMGLGVPESAEPVGTHARPQPPGAELPAPPANSPPEPTIAHAHSVDPRDAWMLFVKQSDKGINSKRRSKARRLKLGLPGPPGPPGPQGPPGPFIPSEVLLKEFQLLLKGAVRQRESHLEHCTRDLTTPASGSPSRVPAAQELDSQDPGALLALLAATLAQGPRAPRVEAAFHCRLRRDVQVDRRALHELGIYYLPEVEGAFHRGPGLNLTSGQYTAPVAGFYALAATLHHSPSSQERDQHDPGIVCAC

Target ORF information:

RefSeq Version	XM_030253764.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus erythroferrone (Erfe), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253764.1

ORF Insert Sequence:

ATGGCCTCGA CCCGCCGCCC CGTCGGAGCT CGCACGCTGC TCGCCTGCGC CAGCCTACTC 
GCCGCCATGG GCCTCGGTGT CCCTGAGTCC GCGGAGCCCG TGGGGACTCA TGCACGCCCG 
CAGCCGCCCG GGGCCGAGCT GCCCGCCCCG CCAGCCAACA GCCCGCCGGA ACCCACCATT 
GCGCATGCAC ACAGTGTGGA TCCCCGGGAT GCTTGGATGC TGTTCGTCAA GCAGAGTGAC 
AAGGGGATCA ACAGTAAGAG GAGGAGCAAA GCCAGGAGGC TGAAGCTTGG CCTGCCAGGA 
CCCCCAGGGC CACCAGGTCC TCAGGGCCCC CCAGGCCCCT TTATCCCATC TGAGGTTCTG 
CTGAAGGAGT TCCAGCTGTT GCTGAAAGGC GCAGTACGGC AGCGAGAGAG CCATCTGGAG 
CACTGCACCA GGGATCTCAC TACACCAGCC TCGGGTAGCC CTTCCCGTGT CCCAGCCGCC 
CAGGAGCTTG ATAGCCAGGA CCCAGGGGCA TTGTTAGCTC TGCTGGCTGC GACCTTGGCC 
CAGGGCCCGC GGGCACCACG TGTGGAGGCC GCATTCCACT GTCGCTTGCG CCGGGATGTG 
CAGGTGGATC GGCGTGCGTT GCACGAGCTT GGGATCTACT ACCTGCCCGA AGTTGAGGGA 
GCCTTCCACC GGGGCCCAGG CTTGAATCTG ACCAGCGGCC AGTACACCGC ACCTGTGGCT 
GGCTTCTATG CGCTTGCTGC CACTCTGCAC CACTCACCGA GCAGCCAAGA AAGGGACCAA 
CACGACCCCG GGATCGTCTG CGCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Erfe ( XM_006529487.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529550.1 Mus musculus erythroferrone (Erfe), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu28166
Clone ID Related Accession (Same CDS sequence)	XM_006529487.3 , XM_006529487.4
Accession Version	XM_006529487.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	erythroferrone isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529487.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGA CCCGCCGCCC CGTCGGAGCT CGCACGCTGC TCGCCTGCGC CAGCCTACTC 
GCCGCCATGG GCCTCGGTGT CCCTGAGTCC GCGGAGCCCG TGGGGACTCA TGCACGCCCG 
CAGCCGCCCG GGGCCGAGCT GCCCGCCCCG CCAGCCAACA GCCCGCCGGA ACCCACCATT 
GCGCATGCAC ACAGTGTGGA TCCCCGGGAT GCTTGGATGC TGTTCGTCAA GCAGAGTGAC 
AAGGGGATCA ACAGTAAGAG GAGGAGCAAA GCCAGGAGGC TGAAGCTTGG CCTGCCAGGA 
CCCCCAGGGC CACCAGGTCC TCAGGGCCCC CCAGGCCCCT TTATCCCATC TGAGGTTCTG 
CTGAAGGAGT TCCAGCTGTT GCTGAAAGGC GCAGTACGGC AGCGAGAGAG CCATCTGGAG 
CACTGCACCA GGGATCTCAC TACACCAGCC TCGGGTAGCC CTTCCCGTGT CCCAGCCGCC 
CAGGAGCTTG ATAGCCAGGA CCCAGGGGCA TTGTTAGCTC TGCTGGCTGC GACCTTGGCC 
CAGGGCCCGC GGGCACCACG TGTGGAGGCC GCATTCCACT GTCGCTTGCG CCGGGATGTG 
CAGGTGGATC GGCGTGCGTT GCACGAGCTT GGGATCTACT ACCTGCACTC ACCGAGCAGC 
CAAGAAAGGG ACCAACACGA CCCCGGGATC GTCTGCGCCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529550.1
CDS	177..881
Translation	MASTRRPVGARTLLACASLLAAMGLGVPESAEPVGTHARPQPPGAELPAPPANSPPEPTIAHAHSVDPRDAWMLFVKQSDKGINSKRRSKARRLKLGLPGPPGPPGPQGPPGPFIPSEVLLKEFQLLLKGAVRQRESHLEHCTRDLTTPASGSPSRVPAAQELDSQDPGALLALLAATLAQGPRAPRVEAAFHCRLRRDVQVDRRALHELGIYYLHSPSSQERDQHDPGIVCAC

Target ORF information:

RefSeq Version	XM_006529487.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus erythroferrone (Erfe), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529487.4

ORF Insert Sequence:

ATGGCCTCGA CCCGCCGCCC CGTCGGAGCT CGCACGCTGC TCGCCTGCGC CAGCCTACTC 
GCCGCCATGG GCCTCGGTGT CCCTGAGTCC GCGGAGCCCG TGGGGACTCA TGCACGCCCG 
CAGCCGCCCG GGGCCGAGCT GCCCGCCCCG CCAGCCAACA GCCCGCCGGA ACCCACCATT 
GCGCATGCAC ACAGTGTGGA TCCCCGGGAT GCTTGGATGC TGTTCGTCAA GCAGAGTGAC 
AAGGGGATCA ACAGTAAGAG GAGGAGCAAA GCCAGGAGGC TGAAGCTTGG CCTGCCAGGA 
CCCCCAGGGC CACCAGGTCC TCAGGGCCCC CCAGGCCCCT TTATCCCATC TGAGGTTCTG 
CTGAAGGAGT TCCAGCTGTT GCTGAAAGGC GCAGTACGGC AGCGAGAGAG CCATCTGGAG 
CACTGCACCA GGGATCTCAC TACACCAGCC TCGGGTAGCC CTTCCCGTGT CCCAGCCGCC 
CAGGAGCTTG ATAGCCAGGA CCCAGGGGCA TTGTTAGCTC TGCTGGCTGC GACCTTGGCC 
CAGGGCCCGC GGGCACCACG TGTGGAGGCC GCATTCCACT GTCGCTTGCG CCGGGATGTG 
CAGGTGGATC GGCGTGCGTT GCACGAGCTT GGGATCTACT ACCTGCACTC ACCGAGCAGC 
CAAGAAAGGG ACCAACACGA CCCCGGGATC GTCTGCGCCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Erfe ( XM_006529484.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529547.1 Mus musculus family with sequence similarity 132, member B (Fam132b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu28164
Clone ID Related Accession (Same CDS sequence)	XM_006529484.3 , XM_006529484.4
Accession Version	XM_006529484.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	erythroferrone isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529484.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGA CCCGCCGCCC CGTCGGAGCT CGCACGCTGC TCGCCTGCGC CAGCCTACTC 
GCCGCCATGG GCCTCGGTGT CCCTGAGTCC GCGGAGCCCG TGGGGACTCA TGCACGCCCG 
CAGCCGCCCG GGGCCGAGCT GCCCGCCCCG CCAGCCAACA GCCCGCCGGA ACCCACCATT 
GCGCATGCAC ACAGTGTGGA TCCCCGGGAT GCTTGGATGC TGTTCGTCAA GCAGAGTGAC 
AAGGGGATCA ACAGTAAGAG GAGGAGCAAA GCCAGGAGGC TGAAGCTTGG CCTGCCAGGA 
CCCCCAGGGC CACCAGGTCC TCAGGGCCCC CCAGGCCCCT TTATCCCATC TGAGGTTCTG 
CTGAAGGAGT TCCAGCTGTT GCTGAAAGGC GCAGTACGGC AGCGAGAGAG CCATCTGGAG 
CACTGCACCA GGGATCTCAC TACACCAGCC TCGGGTAGCC CTTCCCGTGT CCCAGCCGCC 
CAGGAGCTTG ATAGCCAGGA CCCAGGGGCA TTGTTAGCTC TGCTGGCTGC GACCTTGGCC 
CAGGGCCCGC GGGCACCACG TGTGGAGGCC GCATTCCACT GTCGCTTGCG CCGGGATGTG 
CAGGTGGATC GGCGTGCGTT GCACGAGCTT GGGATCTACT ACCTGCCCGA AGTTGAGGGA 
GCCTTCCACC GGGGCCCAGG CTTGAATCTG ACCAGCGGCC AGTACACCGC ACCTGTGGCT 
GGCTTCTATG CGCTTGCTGC CACTCTGCAC GTGGCACTCA CCGAGCAGCC AAGAAAGGGA 
CCAACACGAC CCCGGGATCG TCTGCGCCTG CTGATCTGCA TCCAGTCTCT CTGCCAGCAC 
AATGCCTCCC TGGAGACTGT GATGGGGCTG GAGAACAGCA GCGAGCTCTT CACCATCTCA 
GTAAATGGTG TCCTCTATCT ACAGGCAGGA CACTACACTT CTGTCTTCTT GGACAATGCC 
AGCGGCTCCT CCCTCACGCT GACACCAGGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529547.1
CDS	317..1309
Translation	MASTRRPVGARTLLACASLLAAMGLGVPESAEPVGTHARPQPPGAELPAPPANSPPEPTIAHAHSVDPRDAWMLFVKQSDKGINSKRRSKARRLKLGLPGPPGPPGPQGPPGPFIPSEVLLKEFQLLLKGAVRQRESHLEHCTRDLTTPASGSPSRVPAAQELDSQDPGALLALLAATLAQGPRAPRVEAAFHCRLRRDVQVDRRALHELGIYYLPEVEGAFHRGPGLNLTSGQYTAPVAGFYALAATLHVALTEQPRKGPTRPRDRLRLLICIQSLCQHNASLETVMGLENSSELFTISVNGVLYLQAGHYTSVFLDNASGSSLTLTPG

Target ORF information:

RefSeq Version	XM_006529484.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus family with sequence similarity 132, member B (Fam132b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529484.3

ORF Insert Sequence:

ATGGCCTCGA CCCGCCGCCC CGTCGGAGCT CGCACGCTGC TCGCCTGCGC CAGCCTACTC 
GCCGCCATGG GCCTCGGTGT CCCTGAGTCC GCGGAGCCCG TGGGGACTCA TGCACGCCCG 
CAGCCGCCCG GGGCCGAGCT GCCCGCCCCG CCAGCCAACA GCCCGCCGGA ACCCACCATT 
GCGCATGCAC ACAGTGTGGA TCCCCGGGAT GCTTGGATGC TGTTCGTCAA GCAGAGTGAC 
AAGGGGATCA ACAGTAAGAG GAGGAGCAAA GCCAGGAGGC TGAAGCTTGG CCTGCCAGGA 
CCCCCAGGGC CACCAGGTCC TCAGGGCCCC CCAGGCCCCT TTATCCCATC TGAGGTTCTG 
CTGAAGGAGT TCCAGCTGTT GCTGAAAGGC GCAGTACGGC AGCGAGAGAG CCATCTGGAG 
CACTGCACCA GGGATCTCAC TACACCAGCC TCGGGTAGCC CTTCCCGTGT CCCAGCCGCC 
CAGGAGCTTG ATAGCCAGGA CCCAGGGGCA TTGTTAGCTC TGCTGGCTGC GACCTTGGCC 
CAGGGCCCGC GGGCACCACG TGTGGAGGCC GCATTCCACT GTCGCTTGCG CCGGGATGTG 
CAGGTGGATC GGCGTGCGTT GCACGAGCTT GGGATCTACT ACCTGCCCGA AGTTGAGGGA 
GCCTTCCACC GGGGCCCAGG CTTGAATCTG ACCAGCGGCC AGTACACCGC ACCTGTGGCT 
GGCTTCTATG CGCTTGCTGC CACTCTGCAC GTGGCACTCA CCGAGCAGCC AAGAAAGGGA 
CCAACACGAC CCCGGGATCG TCTGCGCCTG CTGATCTGCA TCCAGTCTCT CTGCCAGCAC 
AATGCCTCCC TGGAGACTGT GATGGGGCTG GAGAACAGCA GCGAGCTCTT CACCATCTCA 
GTAAATGGTG TCCTCTATCT ACAGGCAGGA CACTACACTT CTGTCTTCTT GGACAATGCC 
AGCGGCTCCT CCCTCACGCT GACACCAGGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Erfe ( XM_006529485.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529548.1 Mus musculus family with sequence similarity 132, member B (Fam132b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu28165
Clone ID Related Accession (Same CDS sequence)	XM_006529485.3 , XM_006529485.4 , XM_030253764.1
Accession Version	XM_006529485.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	erythroferrone isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529485.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGA CCCGCCGCCC CGTCGGAGCT CGCACGCTGC TCGCCTGCGC CAGCCTACTC 
GCCGCCATGG GCCTCGGTGT CCCTGAGTCC GCGGAGCCCG TGGGGACTCA TGCACGCCCG 
CAGCCGCCCG GGGCCGAGCT GCCCGCCCCG CCAGCCAACA GCCCGCCGGA ACCCACCATT 
GCGCATGCAC ACAGTGTGGA TCCCCGGGAT GCTTGGATGC TGTTCGTCAA GCAGAGTGAC 
AAGGGGATCA ACAGTAAGAG GAGGAGCAAA GCCAGGAGGC TGAAGCTTGG CCTGCCAGGA 
CCCCCAGGGC CACCAGGTCC TCAGGGCCCC CCAGGCCCCT TTATCCCATC TGAGGTTCTG 
CTGAAGGAGT TCCAGCTGTT GCTGAAAGGC GCAGTACGGC AGCGAGAGAG CCATCTGGAG 
CACTGCACCA GGGATCTCAC TACACCAGCC TCGGGTAGCC CTTCCCGTGT CCCAGCCGCC 
CAGGAGCTTG ATAGCCAGGA CCCAGGGGCA TTGTTAGCTC TGCTGGCTGC GACCTTGGCC 
CAGGGCCCGC GGGCACCACG TGTGGAGGCC GCATTCCACT GTCGCTTGCG CCGGGATGTG 
CAGGTGGATC GGCGTGCGTT GCACGAGCTT GGGATCTACT ACCTGCCCGA AGTTGAGGGA 
GCCTTCCACC GGGGCCCAGG CTTGAATCTG ACCAGCGGCC AGTACACCGC ACCTGTGGCT 
GGCTTCTATG CGCTTGCTGC CACTCTGCAC CACTCACCGA GCAGCCAAGA AAGGGACCAA 
CACGACCCCG GGATCGTCTG CGCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529548.1
CDS	319..1128
Translation	MASTRRPVGARTLLACASLLAAMGLGVPESAEPVGTHARPQPPGAELPAPPANSPPEPTIAHAHSVDPRDAWMLFVKQSDKGINSKRRSKARRLKLGLPGPPGPPGPQGPPGPFIPSEVLLKEFQLLLKGAVRQRESHLEHCTRDLTTPASGSPSRVPAAQELDSQDPGALLALLAATLAQGPRAPRVEAAFHCRLRRDVQVDRRALHELGIYYLPEVEGAFHRGPGLNLTSGQYTAPVAGFYALAATLHHSPSSQERDQHDPGIVCAC

Target ORF information:

RefSeq Version	XM_006529485.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus family with sequence similarity 132, member B (Fam132b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529485.3

ORF Insert Sequence:

ATGGCCTCGA CCCGCCGCCC CGTCGGAGCT CGCACGCTGC TCGCCTGCGC CAGCCTACTC 
GCCGCCATGG GCCTCGGTGT CCCTGAGTCC GCGGAGCCCG TGGGGACTCA TGCACGCCCG 
CAGCCGCCCG GGGCCGAGCT GCCCGCCCCG CCAGCCAACA GCCCGCCGGA ACCCACCATT 
GCGCATGCAC ACAGTGTGGA TCCCCGGGAT GCTTGGATGC TGTTCGTCAA GCAGAGTGAC 
AAGGGGATCA ACAGTAAGAG GAGGAGCAAA GCCAGGAGGC TGAAGCTTGG CCTGCCAGGA 
CCCCCAGGGC CACCAGGTCC TCAGGGCCCC CCAGGCCCCT TTATCCCATC TGAGGTTCTG 
CTGAAGGAGT TCCAGCTGTT GCTGAAAGGC GCAGTACGGC AGCGAGAGAG CCATCTGGAG 
CACTGCACCA GGGATCTCAC TACACCAGCC TCGGGTAGCC CTTCCCGTGT CCCAGCCGCC 
CAGGAGCTTG ATAGCCAGGA CCCAGGGGCA TTGTTAGCTC TGCTGGCTGC GACCTTGGCC 
CAGGGCCCGC GGGCACCACG TGTGGAGGCC GCATTCCACT GTCGCTTGCG CCGGGATGTG 
CAGGTGGATC GGCGTGCGTT GCACGAGCTT GGGATCTACT ACCTGCCCGA AGTTGAGGGA 
GCCTTCCACC GGGGCCCAGG CTTGAATCTG ACCAGCGGCC AGTACACCGC ACCTGTGGCT 
GGCTTCTATG CGCTTGCTGC CACTCTGCAC CACTCACCGA GCAGCCAAGA AAGGGACCAA 
CACGACCCCG GGATCGTCTG CGCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Erfe ( XM_006529487.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529550.1 Mus musculus family with sequence similarity 132, member B (Fam132b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu28166
Clone ID Related Accession (Same CDS sequence)	XM_006529487.3 , XM_006529487.4
Accession Version	XM_006529487.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	erythroferrone isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529487.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGA CCCGCCGCCC CGTCGGAGCT CGCACGCTGC TCGCCTGCGC CAGCCTACTC 
GCCGCCATGG GCCTCGGTGT CCCTGAGTCC GCGGAGCCCG TGGGGACTCA TGCACGCCCG 
CAGCCGCCCG GGGCCGAGCT GCCCGCCCCG CCAGCCAACA GCCCGCCGGA ACCCACCATT 
GCGCATGCAC ACAGTGTGGA TCCCCGGGAT GCTTGGATGC TGTTCGTCAA GCAGAGTGAC 
AAGGGGATCA ACAGTAAGAG GAGGAGCAAA GCCAGGAGGC TGAAGCTTGG CCTGCCAGGA 
CCCCCAGGGC CACCAGGTCC TCAGGGCCCC CCAGGCCCCT TTATCCCATC TGAGGTTCTG 
CTGAAGGAGT TCCAGCTGTT GCTGAAAGGC GCAGTACGGC AGCGAGAGAG CCATCTGGAG 
CACTGCACCA GGGATCTCAC TACACCAGCC TCGGGTAGCC CTTCCCGTGT CCCAGCCGCC 
CAGGAGCTTG ATAGCCAGGA CCCAGGGGCA TTGTTAGCTC TGCTGGCTGC GACCTTGGCC 
CAGGGCCCGC GGGCACCACG TGTGGAGGCC GCATTCCACT GTCGCTTGCG CCGGGATGTG 
CAGGTGGATC GGCGTGCGTT GCACGAGCTT GGGATCTACT ACCTGCACTC ACCGAGCAGC 
CAAGAAAGGG ACCAACACGA CCCCGGGATC GTCTGCGCCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529550.1
CDS	321..1025
Translation	MASTRRPVGARTLLACASLLAAMGLGVPESAEPVGTHARPQPPGAELPAPPANSPPEPTIAHAHSVDPRDAWMLFVKQSDKGINSKRRSKARRLKLGLPGPPGPPGPQGPPGPFIPSEVLLKEFQLLLKGAVRQRESHLEHCTRDLTTPASGSPSRVPAAQELDSQDPGALLALLAATLAQGPRAPRVEAAFHCRLRRDVQVDRRALHELGIYYLHSPSSQERDQHDPGIVCAC

Target ORF information:

RefSeq Version	XM_006529487.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus family with sequence similarity 132, member B (Fam132b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529487.3

ORF Insert Sequence:

ATGGCCTCGA CCCGCCGCCC CGTCGGAGCT CGCACGCTGC TCGCCTGCGC CAGCCTACTC 
GCCGCCATGG GCCTCGGTGT CCCTGAGTCC GCGGAGCCCG TGGGGACTCA TGCACGCCCG 
CAGCCGCCCG GGGCCGAGCT GCCCGCCCCG CCAGCCAACA GCCCGCCGGA ACCCACCATT 
GCGCATGCAC ACAGTGTGGA TCCCCGGGAT GCTTGGATGC TGTTCGTCAA GCAGAGTGAC 
AAGGGGATCA ACAGTAAGAG GAGGAGCAAA GCCAGGAGGC TGAAGCTTGG CCTGCCAGGA 
CCCCCAGGGC CACCAGGTCC TCAGGGCCCC CCAGGCCCCT TTATCCCATC TGAGGTTCTG 
CTGAAGGAGT TCCAGCTGTT GCTGAAAGGC GCAGTACGGC AGCGAGAGAG CCATCTGGAG 
CACTGCACCA GGGATCTCAC TACACCAGCC TCGGGTAGCC CTTCCCGTGT CCCAGCCGCC 
CAGGAGCTTG ATAGCCAGGA CCCAGGGGCA TTGTTAGCTC TGCTGGCTGC GACCTTGGCC 
CAGGGCCCGC GGGCACCACG TGTGGAGGCC GCATTCCACT GTCGCTTGCG CCGGGATGTG 
CAGGTGGATC GGCGTGCGTT GCACGAGCTT GGGATCTACT ACCTGCACTC ACCGAGCAGC 
CAAGAAAGGG ACCAACACGA CCCCGGGATC GTCTGCGCCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hepcidin is regulated by promoter-associated histone acetylation and HDAC3.
Nature communications8(1)403(2017 09)
Pasricha SR,Lim PJ,Duarte TL,Casu C,Oosterhuis D,Mleczko-Sanecka K,Suciu M,Da Silva AR,Al-Hourani K,Arezes J,McHugh K,Gooding S,Frost JN,Wray K,Santos A,Porto G,Repapi E,Gray N,Draper SJ,Ashley N,Soilleux E,Olinga P,Muckenthaler MU,Hughes JR,Rivella S,Milne TA,Armitage AE,Drakesmith H

Effect of erythropoietin administration on proteins participating in iron homeostasis in Tmprss6-mutated mask mice.
PloS one12(10)e0186844(2017)
Fr?dlov? J,Rychtar??kov? Z,Gurieva I,Vokurka M,Truksa J,Krijt J

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Erfe cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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