Hhat cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hhat
Entrez Gene ID	226861
Full Name	hedgehog acyltransferase
Synonyms	2810432O22Rik,AI462858,AP-2CRE,Skn,Tg(TFAP2A-cre)1Will
General protein information	Preferred Names hedgehog acyltransferase Names protein-cysteine N-palmitoyltransferase HHAT skinny hedgehog protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 H6

mRNA and Protein(s)

mRNA	Protein	Name
XM_017320220.2	XP_017175709.1	protein-cysteine N-palmitoyltransferase HHAT isoform X2
XM_017320221.2	XP_017175710.1	protein-cysteine N-palmitoyltransferase HHAT isoform X3
XM_017320220.1	XP_017175709.1	protein-cysteine N-palmitoyltransferase HHAT isoform X2
XM_017320221.1	XP_017175710.1	protein-cysteine N-palmitoyltransferase HHAT isoform X3
XM_011238934.3	XP_011237236.1	protein-cysteine N-palmitoyltransferase HHAT isoform X1
XM_011238934.2	XP_011237236.1	protein-cysteine N-palmitoyltransferase HHAT isoform X1
NM_144881.4	NP_659130.2	protein-cysteine N-palmitoyltransferase HHAT precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	HHAT	XP_537140.3
Caenorhabditis elegans (roundworm)	hhat-1	NP_001022489.1
Pan troglodytes (chimpanzee)	HHAT	XP_001169380.1
	LOC712333	XP_001109445.2
Bos taurus (cattle)	HHAT	XP_002694341.2
Mus musculus (house mouse)	Hhat	NP_659130.2
Gallus gallus (chicken)	HHAT	XP_001234561.1
Drosophila melanogaster (fruit fly)	rasp	NP_523898.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002602	XP_312335.5
Caenorhabditis elegans (roundworm)	hhat-2	NP_001255840.1
Xenopus tropicalis (tropical clawed frog)	hhat	XP_002934723.2
Homo sapiens (human)	HHAT	NP_001164051.1
Rattus norvegicus (Norway rat)	Hhat	XP_006250560.1
Danio rerio (zebrafish)	hhat	NP_001108362.1

Gene Ontology
Bibliography

Related articles in PubMed

Hydronephrosis in the Wnt5a-ablated kidney is caused by an abnormal ureter-bladder connection.
Yun K, Perantoni AO
Differentiation; research in biological diversity941-7(2017 Mar - Apr)

Ventral neural patterning in the absence of a Shh activity gradient from the floorplate.
Iulianella A, Sakai D, Kurosaka H, Trainor PA
Developmental dynamics : an official publication of the American Association of Anatomists247(1)170-184(2018 Jan)

Cranial nerve development requires co-ordinated Shh and canonical Wnt signaling.
Kurosaka H, Trainor PA, Leroux-Berger M, Iulianella A
PloS one10(3)e0120821(2015)

Loss of function mutation in the palmitoyl-transferase HHAT leads to syndromic 46,XY disorder of sex development by impeding Hedgehog protein palmitoylation and signaling.
Callier P, Calvel P, Matevossian A, Makrythanasis P, Bernard P, Kurosaka H, Vannier A, Thauvin-Robinet C, Borel C, Mazaud-Guittot S, Rolland A, Desdoits-Lethimonier C, Guipponi M, Zimmermann C, St?vant I, Kuhne F, Conne B, Santoni F, Lambert S, Huet F, Mugneret F, Jaruzelska J, Faivre L, Wilhelm D, J?gou B, Trainor PA, Resh MD, Antonarakis SE, Nef S
PLoS genetics10(5)e1004340(2014 May)

Disrupting hedgehog and WNT signaling interactions promotes cleft lip pathogenesis.
Kurosaka H, Iulianella A, Williams T, Trainor PA
The Journal of clinical investigation124(4)1660-71(2014 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mutations in Hedgehog acyltransferase (Hhat) perturb Hedgehog signaling, resulting in severe acrania-holoprosencephaly-agnathia craniofacial defects.
Title: Mutations in Hedgehog acyltransferase (Hhat) perturb Hedgehog signaling, resulting in severe acrania-holoprosencephaly-agnathia craniofacial defects.

The following Hhat gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hhat cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu21722	NM_144881.4 Latest version!	Mus musculus hedgehog acyltransferase (Hhat), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu77107	XM_017320220.2 Latest version!	Mus musculus hedgehog acyltransferase (Hhat), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu77108	XM_017320221.2 Latest version!	Mus musculus hedgehog acyltransferase (Hhat), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu73314	XM_011238934.3 Latest version!	Mus musculus hedgehog acyltransferase (Hhat), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu77107	XM_017320220.1	Mus musculus hedgehog acyltransferase (Hhat), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu77108	XM_017320221.1	Mus musculus hedgehog acyltransferase (Hhat), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu73314	XM_011238934.2	Mus musculus hedgehog acyltransferase (Hhat), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hhat ( NM_144881.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_659130.2 Mus musculus hedgehog acyltransferase (Hhat), mRNA.

Price & Availability↑

CloneID	OMu21722
Clone ID Related Accession (Same CDS sequence)	NM_144881.4
Accession Version	NM_144881.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Mus musculus(house mouse)
Product	protein-cysteine N-palmitoyltransferase HHAT precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY087228.1 and AK028230.1. On May 16, 2009 this sequence version replaced NM_144881.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK028230.1, AK172126.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCCCG GGTGGGAACT GACTCTCTGC CTGCTTGTTT CCTTGGGTTT CCACTTCCGC 
TCTTTCTATG AAGTTTACAA AGTCTCCAGA GAGCATGAAG AGGAACTGGA TCAAGAATTT 
GAGCTGGAGA TGGACACTCT ATTTGGAGGA CTGAAGAAGG ACCCGACTGA TTTTGAGTGG 
AACTTCTGGA TGGAATGGGG GAAGCGGAGG CTGGTGTGGC TCTTCATTGG CCATATGGCT 
GTGTCTCAGT TGGCCACGTT ACTCACAAAG AAGCACAGAC CCTGGATTGT CATGGTCTAT 
GGAATGTGGG CCTGCTGGTG TGTGCTGGGG GCGCCAGGCG TGGTCATGGT CCTTCTCCAC 
AGCACCATCG CGTTCTGCGT GGCTCAGTTC CGATCCGTGC TCCTGTCCTG GCTGTGTTCT 
CTCCTTCTGC TCTCCACCCT AAGGCTGCAA AGTGTGGAGG AAGTTAAGAG AAGGTGGTAC 
AAGACAGAGA ACGAGTATTA CCTGCTCCAG TTTACGCTCA CTGTTCGCTG CCTGTACTAC 
ACCAGCTTCA GCCTGGAGCT GTGCCGGCAG CCTCCGTCTG CACAACCTAC TCCATCTGCG 
CAAGGTGCCT CCCATTCCTA CCCGTGGCTG CTGACCTACG TCTTCTATTA TCCCGTCTTC 
CACAACGGGC CCATCCTTAA CTTCCCGGAG TTCTTCAGAC AGATGCAGCA GCCAGAGCTC 
AACTCTCTGC AGCACAGCCT CTGCATTGTA GCCAAGGGCC TGGGTCGTCT GCTCTGCTGG 
TGGTGGCTGG CTGAACTGAT GGTACACCTC ATGTACATGC ATGCCCTCTA CAGCAGCGCC 
CCCCTCCTGG AGAGCGTTTC CTGCTGGACC TTAGGAGGAC TGGCGCTGGC CCAAGTGCTC 
TTCTTCTATG TGAAGTACCT GGTGCTCTTT GGTGTCCCGG CTCTGCTCAT GCGCCTGGAT 
GGACTCACGC CACCACCTCT TCCCCGCTGC GTGAGCACCA TGTTCAGTTT TACCGGGATG 
TGGAGGTATT TTGATGTTGG ACTACACAAT TTCTTAATCA GGTATGTGTA CATCCCACTG 
GGCGGGTCCC AGCATGGCCT GTTGGGGACA CTGCTTTCTA CTGCAACAAC GTTTGCGTTC 
GTGAGCTACT GGCATGGTAG CTATGAGGAC CTCTGGTGCT GGGCAGCGCT CAACTGGCTG 
GGAGTCACTG TGGAGAGTGG GGTCCGGAGG CTGCTAGAGA CACCCTGTGT CCGGGAGACT 
TTGGCCCGGC ACCTCTCCCC ACAAGCCCAC CATCGACTGC ACGCTCTGCT TGCCGCCTGC 
TCTACCTCCA TGCTCATCCT CTTCAACCTG GTGTTCCTCG GGGGAATTCA AGTTGGGAAA 
ACCTACTGGA ACAGGATCTT CCTGCAAGGG TGGCCATGGG TGACTCTGTC TGTCCTGGGA 
TTCCTGTACT GCTATTCTCA CGTGGACATT GCTTGGGCTC AGACATACAC AGTTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_659130.2
CDS	280..1779
Translation	MLPGWELTLCLLVSLGFHFRSFYEVYKVSREHEEELDQEFELEMDTLFGGLKKDPTDFEWNFWMEWGKRRLVWLFIGHMAVSQLATLLTKKHRPWIVMVYGMWACWCVLGAPGVVMVLLHSTIAFCVAQFRSVLLSWLCSLLLLSTLRLQSVEEVKRRWYKTENEYYLLQFTLTVRCLYYTSFSLELCRQPPSAQPTPSAQGASHSYPWLLTYVFYYPVFHNGPILNFPEFFRQMQQPELNSLQHSLCIVAKGLGRLLCWWWLAELMVHLMYMHALYSSAPLLESVSCWTLGGLALAQVLFFYVKYLVLFGVPALLMRLDGLTPPPLPRCVSTMFSFTGMWRYFDVGLHNFLIRYVYIPLGGSQHGLLGTLLSTATTFAFVSYWHGSYEDLWCWAALNWLGVTVESGVRRLLETPCVRETLARHLSPQAHHRLHALLAACSTSMLILFNLVFLGGIQVGKTYWNRIFLQGWPWVTLSVLGFLYCYSHVDIAWAQTYTVL

Target ORF information:

RefSeq Version	NM_144881.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus hedgehog acyltransferase (Hhat), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144881.4

ORF Insert Sequence:

ATGCTGCCCG GGTGGGAACT GACTCTCTGC CTGCTTGTTT CCTTGGGTTT CCACTTCCGC 
TCTTTCTATG AAGTTTACAA AGTCTCCAGA GAGCATGAAG AGGAACTGGA TCAAGAATTT 
GAGCTGGAGA TGGACACTCT ATTTGGAGGA CTGAAGAAGG ACCCGACTGA TTTTGAGTGG 
AACTTCTGGA TGGAATGGGG GAAGCGGAGG CTGGTGTGGC TCTTCATTGG CCATATGGCT 
GTGTCTCAGT TGGCCACGTT ACTCACAAAG AAGCACAGAC CCTGGATTGT CATGGTCTAT 
GGAATGTGGG CCTGCTGGTG TGTGCTGGGG GCGCCAGGCG TGGTCATGGT CCTTCTCCAC 
AGCACCATCG CGTTCTGCGT GGCTCAGTTC CGATCCGTGC TCCTGTCCTG GCTGTGTTCT 
CTCCTTCTGC TCTCCACCCT AAGGCTGCAA AGTGTGGAGG AAGTTAAGAG AAGGTGGTAC 
AAGACAGAGA ACGAGTATTA CCTGCTCCAG TTTACGCTCA CTGTTCGCTG CCTGTACTAC 
ACCAGCTTCA GCCTGGAGCT GTGCCGGCAG CCTCCGTCTG CACAACCTAC TCCATCTGCG 
CAAGGTGCCT CCCATTCCTA CCCGTGGCTG CTGACCTACG TCTTCTATTA TCCCGTCTTC 
CACAACGGGC CCATCCTTAA CTTCCCGGAG TTCTTCAGAC AGATGCAGCA GCCAGAGCTC 
AACTCTCTGC AGCACAGCCT CTGCATTGTA GCCAAGGGCC TGGGTCGTCT GCTCTGCTGG 
TGGTGGCTGG CTGAACTGAT GGTACACCTC ATGTACATGC ATGCCCTCTA CAGCAGCGCC 
CCCCTCCTGG AGAGCGTTTC CTGCTGGACC TTAGGAGGAC TGGCGCTGGC CCAAGTGCTC 
TTCTTCTATG TGAAGTACCT GGTGCTCTTT GGTGTCCCGG CTCTGCTCAT GCGCCTGGAT 
GGACTCACGC CACCACCTCT TCCCCGCTGC GTGAGCACCA TGTTCAGTTT TACCGGGATG 
TGGAGGTATT TTGATGTTGG ACTACACAAT TTCTTAATCA GGTATGTGTA CATCCCACTG 
GGCGGGTCCC AGCATGGCCT GTTGGGGACA CTGCTTTCTA CTGCAACAAC GTTTGCGTTC 
GTGAGCTACT GGCATGGTAG CTATGAGGAC CTCTGGTGCT GGGCAGCGCT CAACTGGCTG 
GGAGTCACTG TGGAGAGTGG GGTCCGGAGG CTGCTAGAGA CACCCTGTGT CCGGGAGACT 
TTGGCCCGGC ACCTCTCCCC ACAAGCCCAC CATCGACTGC ACGCTCTGCT TGCCGCCTGC 
TCTACCTCCA TGCTCATCCT CTTCAACCTG GTGTTCCTCG GGGGAATTCA AGTTGGGAAA 
ACCTACTGGA ACAGGATCTT CCTGCAAGGG TGGCCATGGG TGACTCTGTC TGTCCTGGGA 
TTCCTGTACT GCTATTCTCA CGTGGACATT GCTTGGGCTC AGACATACAC AGTTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hhat ( XM_011238934.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237236.1 Mus musculus hedgehog acyltransferase (Hhat), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu73314
Clone ID Related Accession (Same CDS sequence)	XM_011238934.3 , XM_011238934.2
Accession Version	XM_011238934.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein-cysteine N-palmitoyltransferase HHAT isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238934.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCTG AGAGGCAGCT CAGTGGCAGA GCTATATCTG TGCCTAAGTG GGCGGCGGCC 
CTGGGTTCAA TCCCTTACAC TGGAGACTGG GGTTCCACAG TCCTTAGGGA CAGAGGCAGG 
ATCTGGCCCT GTGACTGTGT ATTTCTGACC TGCTATCTCA GGCTGATCAG GGAACAAGAA 
CGGGAGCTAG ATTGTTTTCC TGGGGGTTGG GGCGAGGAGT CCCAGCTGAC TGGGAAGTCA 
GAAGTGAAGG GATGCTCATC GCCGAGCCAC ACTGGCACTG TGGTGGCCCT CCCATTCGCA 
CACAGACCCT GGATTGTCAT GGTCTATGGA ATGTGGGCCT GCTGGTGTGT GCTGGGGGCG 
CCAGGCGTGG TCATGGTCCT TCTCCACAGC ACCATCGCGT TCTGCGTGGC TCAGTTCCGA 
TCCGTGCTCC TGTCCTGGCT GTGTTCTCTC CTTCTGCTCT CCACCCTAAG GCTGCAAAGT 
GTGGAGGAAG TTAAGAGAAG GTGGTACAAG ACAGAGAACG AGTATTACCT GCTCCAGTTT 
ACGCTCACTG TTCGCTGCCT GTACTACACC AGCTTCAGCC TGGAGCTGTG CCGGCAGCCT 
CCGTCTGCAC AACCTACTCC ATCTGCGCAA GGTGCCTCCC ATTCCTACCC GTGGCTGCTG 
ACCTACGTCT TCTATTATCC CGTCTTCCAC AACGGGCCCA TCCTTAACTT CCCGGAGTTC 
TTCAGACAGA TGCAGCAGCC AGAGCTCAAC TCTCTGCAGC ACAGCCTCTG CATTGTAGCC 
AAGGGCCTGG GTCGTCTGCT CTGCTGGTGG TGGCTGGCTG AACTGATGGT ACACCTCATG 
TACATGCATG CCCTCTACAG CAGCGCCCCC CTCCTGGAGA GCGTTTCCTG CTGGACCTTA 
GGAGGACTGG CGCTGGCCCA AGTGCTCTTC TTCTATGTGA AGTACCTGGT GCTCTTTGGT 
GTCCCGGCTC TGCTCATGCG CCTGGATGGA CTCACGCCAC CACCTCTTCC CCGCTGCGTG 
AGCACCATGT TCAGTTTTAC CGGGATGTGG AGGTATTTTG ATGTTGGACT ACACAATTTC 
TTAATCAGGT ATGTGTACAT CCCACTGGGC GGGTCCCAGC ATGGCCTGTT GGGGACACTG 
CTTTCTACTG CAACAACGTT TGCGTTCGTG AGCTACTGGC ATGGTAGCTA TGAGGACCTC 
TGGTGCTGGG CAGCGCTCAA CTGGCTGGGA GTCACTGTGG AGAGTGGGGT CCGGAGGCTG 
CTAGAGACAC CCTGTGTCCG GGAGACTTTG GCCCGGCACC TCTCCCCACA AGCCCACCAT 
CGACTGCACG CTCTGCTTGC CGCCTGCTCT ACCTCCATGC TCATCCTCTT CAACCTGGTG 
TTCCTCGGGG GAATTCAAGT TGGGAAAACC TACTGGAACA GGATCTTCCT GCAAGGGTGG 
CCATGGGTGA CTCTGTCTGT CCTGGGATTC CTGTACTGCT ATTCTCACGT GGACATTGCT 
TGGGCTCAGA CATACACAGT TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237236.1
CDS	109..1635
Translation	MQAERQLSGRAISVPKWAAALGSIPYTGDWGSTVLRDRGRIWPCDCVFLTCYLRLIREQERELDCFPGGWGEESQLTGKSEVKGCSSPSHTGTVVALPFAHRPWIVMVYGMWACWCVLGAPGVVMVLLHSTIAFCVAQFRSVLLSWLCSLLLLSTLRLQSVEEVKRRWYKTENEYYLLQFTLTVRCLYYTSFSLELCRQPPSAQPTPSAQGASHSYPWLLTYVFYYPVFHNGPILNFPEFFRQMQQPELNSLQHSLCIVAKGLGRLLCWWWLAELMVHLMYMHALYSSAPLLESVSCWTLGGLALAQVLFFYVKYLVLFGVPALLMRLDGLTPPPLPRCVSTMFSFTGMWRYFDVGLHNFLIRYVYIPLGGSQHGLLGTLLSTATTFAFVSYWHGSYEDLWCWAALNWLGVTVESGVRRLLETPCVRETLARHLSPQAHHRLHALLAACSTSMLILFNLVFLGGIQVGKTYWNRIFLQGWPWVTLSVLGFLYCYSHVDIAWAQTYTVL

Target ORF information:

RefSeq Version	XM_011238934.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus hedgehog acyltransferase (Hhat), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238934.2

ORF Insert Sequence:

ATGCAGGCTG AGAGGCAGCT CAGTGGCAGA GCTATATCTG TGCCTAAGTG GGCGGCGGCC 
CTGGGTTCAA TCCCTTACAC TGGAGACTGG GGTTCCACAG TCCTTAGGGA CAGAGGCAGG 
ATCTGGCCCT GTGACTGTGT ATTTCTGACC TGCTATCTCA GGCTGATCAG GGAACAAGAA 
CGGGAGCTAG ATTGTTTTCC TGGGGGTTGG GGCGAGGAGT CCCAGCTGAC TGGGAAGTCA 
GAAGTGAAGG GATGCTCATC GCCGAGCCAC ACTGGCACTG TGGTGGCCCT CCCATTCGCA 
CACAGACCCT GGATTGTCAT GGTCTATGGA ATGTGGGCCT GCTGGTGTGT GCTGGGGGCG 
CCAGGCGTGG TCATGGTCCT TCTCCACAGC ACCATCGCGT TCTGCGTGGC TCAGTTCCGA 
TCCGTGCTCC TGTCCTGGCT GTGTTCTCTC CTTCTGCTCT CCACCCTAAG GCTGCAAAGT 
GTGGAGGAAG TTAAGAGAAG GTGGTACAAG ACAGAGAACG AGTATTACCT GCTCCAGTTT 
ACGCTCACTG TTCGCTGCCT GTACTACACC AGCTTCAGCC TGGAGCTGTG CCGGCAGCCT 
CCGTCTGCAC AACCTACTCC ATCTGCGCAA GGTGCCTCCC ATTCCTACCC GTGGCTGCTG 
ACCTACGTCT TCTATTATCC CGTCTTCCAC AACGGGCCCA TCCTTAACTT CCCGGAGTTC 
TTCAGACAGA TGCAGCAGCC AGAGCTCAAC TCTCTGCAGC ACAGCCTCTG CATTGTAGCC 
AAGGGCCTGG GTCGTCTGCT CTGCTGGTGG TGGCTGGCTG AACTGATGGT ACACCTCATG 
TACATGCATG CCCTCTACAG CAGCGCCCCC CTCCTGGAGA GCGTTTCCTG CTGGACCTTA 
GGAGGACTGG CGCTGGCCCA AGTGCTCTTC TTCTATGTGA AGTACCTGGT GCTCTTTGGT 
GTCCCGGCTC TGCTCATGCG CCTGGATGGA CTCACGCCAC CACCTCTTCC CCGCTGCGTG 
AGCACCATGT TCAGTTTTAC CGGGATGTGG AGGTATTTTG ATGTTGGACT ACACAATTTC 
TTAATCAGGT ATGTGTACAT CCCACTGGGC GGGTCCCAGC ATGGCCTGTT GGGGACACTG 
CTTTCTACTG CAACAACGTT TGCGTTCGTG AGCTACTGGC ATGGTAGCTA TGAGGACCTC 
TGGTGCTGGG CAGCGCTCAA CTGGCTGGGA GTCACTGTGG AGAGTGGGGT CCGGAGGCTG 
CTAGAGACAC CCTGTGTCCG GGAGACTTTG GCCCGGCACC TCTCCCCACA AGCCCACCAT 
CGACTGCACG CTCTGCTTGC CGCCTGCTCT ACCTCCATGC TCATCCTCTT CAACCTGGTG 
TTCCTCGGGG GAATTCAAGT TGGGAAAACC TACTGGAACA GGATCTTCCT GCAAGGGTGG 
CCATGGGTGA CTCTGTCTGT CCTGGGATTC CTGTACTGCT ATTCTCACGT GGACATTGCT 
TGGGCTCAGA CATACACAGT TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hhat ( XM_017320220.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175709.1 Mus musculus hedgehog acyltransferase (Hhat), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu77107
Clone ID Related Accession (Same CDS sequence)	XM_017320220.2 , XM_017320220.1
Accession Version	XM_017320220.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1209bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein-cysteine N-palmitoyltransferase HHAT isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017320220.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTATG GAATGTGGGC CTGCTGGTGT GTGCTGGGGG CGCCAGGCGT GGTCATGGTC 
CTTCTCCACA GCACCATCGC GTTCTGCGTG GCTCAGTTCC GATCCGTGCT CCTGTCCTGG 
CTGTGTTCTC TCCTTCTGCT CTCCACCCTA AGGCTGCAAA GTGTGGAGGA AGTTAAGAGA 
AGGTGGTACA AGACAGAGAA CGAGTATTAC CTGCTCCAGT TTACGCTCAC TGTTCGCTGC 
CTGTACTACA CCAGCTTCAG CCTGGAGCTG TGCCGGCAGC CTCCGTCTGC ACAACCTACT 
CCATCTGCGC AAGGTGCCTC CCATTCCTAC CCGTGGCTGC TGACCTACGT CTTCTATTAT 
CCCGTCTTCC ACAACGGGCC CATCCTTAAC TTCCCGGAGT TCTTCAGACA GATGCAGCAG 
CCAGAGCTCA ACTCTCTGCA GCACAGCCTC TGCATTGTAG CCAAGGGCCT GGGTCGTCTG 
CTCTGCTGGT GGTGGCTGGC TGAACTGATG GTACACCTCA TGTACATGCA TGCCCTCTAC 
AGCAGCGCCC CCCTCCTGGA GAGCGTTTCC TGCTGGACCT TAGGAGGACT GGCGCTGGCC 
CAAGTGCTCT TCTTCTATGT GAAGTACCTG GTGCTCTTTG GTGTCCCGGC TCTGCTCATG 
CGCCTGGATG GACTCACGCC ACCACCTCTT CCCCGCTGCG TGAGCACCAT GTTCAGTTTT 
ACCGGGATGT GGAGGTATTT TGATGTTGGA CTACACAATT TCTTAATCAG GTATGTGTAC 
ATCCCACTGG GCGGGTCCCA GCATGGCCTG TTGGGGACAC TGCTTTCTAC TGCAACAACG 
TTTGCGTTCG TGAGCTACTG GCATGGTAGC TATGAGGACC TCTGGTGCTG GGCAGCGCTC 
AACTGGCTGG GAGTCACTGT GGAGAGTGGG GTCCGGAGGC TGCTAGAGAC ACCCTGTGTC 
CGGGAGACTT TGGCCCGGCA CCTCTCCCCA CAAGCCCACC ATCGACTGCA CGCTCTGCTT 
GCCGCCTGCT CTACCTCCAT GCTCATCCTC TTCAACCTGG TGTTCCTCGG GGGAATTCAA 
GTTGGGAAAA CCTACTGGAA CAGGATCTTC CTGCAAGGGT GGCCATGGGT GACTCTGTCT 
GTCCTGGGAT TCCTGTACTG CTATTCTCAC GTGGACATTG CTTGGGCTCA GACATACACA 
GTTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175709.1
CDS	175..1383
Translation	MVYGMWACWCVLGAPGVVMVLLHSTIAFCVAQFRSVLLSWLCSLLLLSTLRLQSVEEVKRRWYKTENEYYLLQFTLTVRCLYYTSFSLELCRQPPSAQPTPSAQGASHSYPWLLTYVFYYPVFHNGPILNFPEFFRQMQQPELNSLQHSLCIVAKGLGRLLCWWWLAELMVHLMYMHALYSSAPLLESVSCWTLGGLALAQVLFFYVKYLVLFGVPALLMRLDGLTPPPLPRCVSTMFSFTGMWRYFDVGLHNFLIRYVYIPLGGSQHGLLGTLLSTATTFAFVSYWHGSYEDLWCWAALNWLGVTVESGVRRLLETPCVRETLARHLSPQAHHRLHALLAACSTSMLILFNLVFLGGIQVGKTYWNRIFLQGWPWVTLSVLGFLYCYSHVDIAWAQTYTVL

Target ORF information:

RefSeq Version	XM_017320220.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus hedgehog acyltransferase (Hhat), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320220.2

ORF Insert Sequence:

ATGGTCTATG GAATGTGGGC CTGCTGGTGT GTGCTGGGGG CGCCAGGCGT GGTCATGGTC 
CTTCTCCACA GCACCATCGC GTTCTGCGTG GCTCAGTTCC GATCCGTGCT CCTGTCCTGG 
CTGTGTTCTC TCCTTCTGCT CTCCACCCTA AGGCTGCAAA GTGTGGAGGA AGTTAAGAGA 
AGGTGGTACA AGACAGAGAA CGAGTATTAC CTGCTCCAGT TTACGCTCAC TGTTCGCTGC 
CTGTACTACA CCAGCTTCAG CCTGGAGCTG TGCCGGCAGC CTCCGTCTGC ACAACCTACT 
CCATCTGCGC AAGGTGCCTC CCATTCCTAC CCGTGGCTGC TGACCTACGT CTTCTATTAT 
CCCGTCTTCC ACAACGGGCC CATCCTTAAC TTCCCGGAGT TCTTCAGACA GATGCAGCAG 
CCAGAGCTCA ACTCTCTGCA GCACAGCCTC TGCATTGTAG CCAAGGGCCT GGGTCGTCTG 
CTCTGCTGGT GGTGGCTGGC TGAACTGATG GTACACCTCA TGTACATGCA TGCCCTCTAC 
AGCAGCGCCC CCCTCCTGGA GAGCGTTTCC TGCTGGACCT TAGGAGGACT GGCGCTGGCC 
CAAGTGCTCT TCTTCTATGT GAAGTACCTG GTGCTCTTTG GTGTCCCGGC TCTGCTCATG 
CGCCTGGATG GACTCACGCC ACCACCTCTT CCCCGCTGCG TGAGCACCAT GTTCAGTTTT 
ACCGGGATGT GGAGGTATTT TGATGTTGGA CTACACAATT TCTTAATCAG GTATGTGTAC 
ATCCCACTGG GCGGGTCCCA GCATGGCCTG TTGGGGACAC TGCTTTCTAC TGCAACAACG 
TTTGCGTTCG TGAGCTACTG GCATGGTAGC TATGAGGACC TCTGGTGCTG GGCAGCGCTC 
AACTGGCTGG GAGTCACTGT GGAGAGTGGG GTCCGGAGGC TGCTAGAGAC ACCCTGTGTC 
CGGGAGACTT TGGCCCGGCA CCTCTCCCCA CAAGCCCACC ATCGACTGCA CGCTCTGCTT 
GCCGCCTGCT CTACCTCCAT GCTCATCCTC TTCAACCTGG TGTTCCTCGG GGGAATTCAA 
GTTGGGAAAA CCTACTGGAA CAGGATCTTC CTGCAAGGGT GGCCATGGGT GACTCTGTCT 
GTCCTGGGAT TCCTGTACTG CTATTCTCAC GTGGACATTG CTTGGGCTCA GACATACACA 
GTTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hhat ( XM_017320220.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175709.1 Mus musculus hedgehog acyltransferase (Hhat), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu77107
Clone ID Related Accession (Same CDS sequence)	XM_017320220.2 , XM_017320220.1
Accession Version	XM_017320220.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1209bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein-cysteine N-palmitoyltransferase HHAT isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTATG GAATGTGGGC CTGCTGGTGT GTGCTGGGGG CGCCAGGCGT GGTCATGGTC 
CTTCTCCACA GCACCATCGC GTTCTGCGTG GCTCAGTTCC GATCCGTGCT CCTGTCCTGG 
CTGTGTTCTC TCCTTCTGCT CTCCACCCTA AGGCTGCAAA GTGTGGAGGA AGTTAAGAGA 
AGGTGGTACA AGACAGAGAA CGAGTATTAC CTGCTCCAGT TTACGCTCAC TGTTCGCTGC 
CTGTACTACA CCAGCTTCAG CCTGGAGCTG TGCCGGCAGC CTCCGTCTGC ACAACCTACT 
CCATCTGCGC AAGGTGCCTC CCATTCCTAC CCGTGGCTGC TGACCTACGT CTTCTATTAT 
CCCGTCTTCC ACAACGGGCC CATCCTTAAC TTCCCGGAGT TCTTCAGACA GATGCAGCAG 
CCAGAGCTCA ACTCTCTGCA GCACAGCCTC TGCATTGTAG CCAAGGGCCT GGGTCGTCTG 
CTCTGCTGGT GGTGGCTGGC TGAACTGATG GTACACCTCA TGTACATGCA TGCCCTCTAC 
AGCAGCGCCC CCCTCCTGGA GAGCGTTTCC TGCTGGACCT TAGGAGGACT GGCGCTGGCC 
CAAGTGCTCT TCTTCTATGT GAAGTACCTG GTGCTCTTTG GTGTCCCGGC TCTGCTCATG 
CGCCTGGATG GACTCACGCC ACCACCTCTT CCCCGCTGCG TGAGCACCAT GTTCAGTTTT 
ACCGGGATGT GGAGGTATTT TGATGTTGGA CTACACAATT TCTTAATCAG GTATGTGTAC 
ATCCCACTGG GCGGGTCCCA GCATGGCCTG TTGGGGACAC TGCTTTCTAC TGCAACAACG 
TTTGCGTTCG TGAGCTACTG GCATGGTAGC TATGAGGACC TCTGGTGCTG GGCAGCGCTC 
AACTGGCTGG GAGTCACTGT GGAGAGTGGG GTCCGGAGGC TGCTAGAGAC ACCCTGTGTC 
CGGGAGACTT TGGCCCGGCA CCTCTCCCCA CAAGCCCACC ATCGACTGCA CGCTCTGCTT 
GCCGCCTGCT CTACCTCCAT GCTCATCCTC TTCAACCTGG TGTTCCTCGG GGGAATTCAA 
GTTGGGAAAA CCTACTGGAA CAGGATCTTC CTGCAAGGGT GGCCATGGGT GACTCTGTCT 
GTCCTGGGAT TCCTGTACTG CTATTCTCAC GTGGACATTG CTTGGGCTCA GACATACACA 
GTTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175709.1
CDS	177..1385
Translation	MVYGMWACWCVLGAPGVVMVLLHSTIAFCVAQFRSVLLSWLCSLLLLSTLRLQSVEEVKRRWYKTENEYYLLQFTLTVRCLYYTSFSLELCRQPPSAQPTPSAQGASHSYPWLLTYVFYYPVFHNGPILNFPEFFRQMQQPELNSLQHSLCIVAKGLGRLLCWWWLAELMVHLMYMHALYSSAPLLESVSCWTLGGLALAQVLFFYVKYLVLFGVPALLMRLDGLTPPPLPRCVSTMFSFTGMWRYFDVGLHNFLIRYVYIPLGGSQHGLLGTLLSTATTFAFVSYWHGSYEDLWCWAALNWLGVTVESGVRRLLETPCVRETLARHLSPQAHHRLHALLAACSTSMLILFNLVFLGGIQVGKTYWNRIFLQGWPWVTLSVLGFLYCYSHVDIAWAQTYTVL

Target ORF information:

RefSeq Version	XM_017320220.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus hedgehog acyltransferase (Hhat), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320220.1

ORF Insert Sequence:

ATGGTCTATG GAATGTGGGC CTGCTGGTGT GTGCTGGGGG CGCCAGGCGT GGTCATGGTC 
CTTCTCCACA GCACCATCGC GTTCTGCGTG GCTCAGTTCC GATCCGTGCT CCTGTCCTGG 
CTGTGTTCTC TCCTTCTGCT CTCCACCCTA AGGCTGCAAA GTGTGGAGGA AGTTAAGAGA 
AGGTGGTACA AGACAGAGAA CGAGTATTAC CTGCTCCAGT TTACGCTCAC TGTTCGCTGC 
CTGTACTACA CCAGCTTCAG CCTGGAGCTG TGCCGGCAGC CTCCGTCTGC ACAACCTACT 
CCATCTGCGC AAGGTGCCTC CCATTCCTAC CCGTGGCTGC TGACCTACGT CTTCTATTAT 
CCCGTCTTCC ACAACGGGCC CATCCTTAAC TTCCCGGAGT TCTTCAGACA GATGCAGCAG 
CCAGAGCTCA ACTCTCTGCA GCACAGCCTC TGCATTGTAG CCAAGGGCCT GGGTCGTCTG 
CTCTGCTGGT GGTGGCTGGC TGAACTGATG GTACACCTCA TGTACATGCA TGCCCTCTAC 
AGCAGCGCCC CCCTCCTGGA GAGCGTTTCC TGCTGGACCT TAGGAGGACT GGCGCTGGCC 
CAAGTGCTCT TCTTCTATGT GAAGTACCTG GTGCTCTTTG GTGTCCCGGC TCTGCTCATG 
CGCCTGGATG GACTCACGCC ACCACCTCTT CCCCGCTGCG TGAGCACCAT GTTCAGTTTT 
ACCGGGATGT GGAGGTATTT TGATGTTGGA CTACACAATT TCTTAATCAG GTATGTGTAC 
ATCCCACTGG GCGGGTCCCA GCATGGCCTG TTGGGGACAC TGCTTTCTAC TGCAACAACG 
TTTGCGTTCG TGAGCTACTG GCATGGTAGC TATGAGGACC TCTGGTGCTG GGCAGCGCTC 
AACTGGCTGG GAGTCACTGT GGAGAGTGGG GTCCGGAGGC TGCTAGAGAC ACCCTGTGTC 
CGGGAGACTT TGGCCCGGCA CCTCTCCCCA CAAGCCCACC ATCGACTGCA CGCTCTGCTT 
GCCGCCTGCT CTACCTCCAT GCTCATCCTC TTCAACCTGG TGTTCCTCGG GGGAATTCAA 
GTTGGGAAAA CCTACTGGAA CAGGATCTTC CTGCAAGGGT GGCCATGGGT GACTCTGTCT 
GTCCTGGGAT TCCTGTACTG CTATTCTCAC GTGGACATTG CTTGGGCTCA GACATACACA 
GTTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hhat ( XM_017320221.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175710.1 Mus musculus hedgehog acyltransferase (Hhat), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu77108
Clone ID Related Accession (Same CDS sequence)	XM_017320221.2 , XM_017320221.1
Accession Version	XM_017320221.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein-cysteine N-palmitoyltransferase HHAT isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017320221.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCAGC CAGAGCTCAA CTCTCTGCAG CACAGCCTCT GCATTGTAGC CAAGGGCCTG 
GGTCGTCTGC TCTGCTGGTG GTGGCTGGCT GAACTGATGG TACACCTCAT GTACATGCAT 
GCCCTCTACA GCAGCGCCCC CCTCCTGGAG AGCGTTTCCT GCTGGACCTT AGGAGGACTG 
GCGCTGGCCC AAGTGCTCTT CTTCTATGTG AAGTACCTGG TGCTCTTTGG TGTCCCGGCT 
CTGCTCATGC GCCTGGATGG ACTCACGCCA CCACCTCTTC CCCGCTGCGT GAGCACCATG 
TTCAGTTTTA CCGGGATGTG GAGGTATTTT GATGTTGGAC TACACAATTT CTTAATCAGG 
TATGTGTACA TCCCACTGGG CGGGTCCCAG CATGGCCTGT TGGGGACACT GCTTTCTACT 
GCAACAACGT TTGCGTTCGT GAGCTACTGG CATGGTAGCT ATGAGGACCT CTGGTGCTGG 
GCAGCGCTCA ACTGGCTGGG AGTCACTGTG GAGAGTGGGG TCCGGAGGCT GCTAGAGACA 
CCCTGTGTCC GGGAGACTTT GGCCCGGCAC CTCTCCCCAC AAGCCCACCA TCGACTGCAC 
GCTCTGCTTG CCGCCTGCTC TACCTCCATG CTCATCCTCT TCAACCTGGT GTTCCTCGGG 
GGAATTCAAG TTGGGAAAAC CTACTGGAAC AGGATCTTCC TGCAAGGGTG GCCATGGGTG 
ACTCTGTCTG TCCTGGGATT CCTGTACTGC TATTCTCACG TGGACATTGC TTGGGCTCAG 
ACATACACAG TTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175710.1
CDS	81..878
Translation	MQQPELNSLQHSLCIVAKGLGRLLCWWWLAELMVHLMYMHALYSSAPLLESVSCWTLGGLALAQVLFFYVKYLVLFGVPALLMRLDGLTPPPLPRCVSTMFSFTGMWRYFDVGLHNFLIRYVYIPLGGSQHGLLGTLLSTATTFAFVSYWHGSYEDLWCWAALNWLGVTVESGVRRLLETPCVRETLARHLSPQAHHRLHALLAACSTSMLILFNLVFLGGIQVGKTYWNRIFLQGWPWVTLSVLGFLYCYSHVDIAWAQTYTVL

Target ORF information:

RefSeq Version	XM_017320221.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus hedgehog acyltransferase (Hhat), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320221.2

ORF Insert Sequence:

ATGCAGCAGC CAGAGCTCAA CTCTCTGCAG CACAGCCTCT GCATTGTAGC CAAGGGCCTG 
GGTCGTCTGC TCTGCTGGTG GTGGCTGGCT GAACTGATGG TACACCTCAT GTACATGCAT 
GCCCTCTACA GCAGCGCCCC CCTCCTGGAG AGCGTTTCCT GCTGGACCTT AGGAGGACTG 
GCGCTGGCCC AAGTGCTCTT CTTCTATGTG AAGTACCTGG TGCTCTTTGG TGTCCCGGCT 
CTGCTCATGC GCCTGGATGG ACTCACGCCA CCACCTCTTC CCCGCTGCGT GAGCACCATG 
TTCAGTTTTA CCGGGATGTG GAGGTATTTT GATGTTGGAC TACACAATTT CTTAATCAGG 
TATGTGTACA TCCCACTGGG CGGGTCCCAG CATGGCCTGT TGGGGACACT GCTTTCTACT 
GCAACAACGT TTGCGTTCGT GAGCTACTGG CATGGTAGCT ATGAGGACCT CTGGTGCTGG 
GCAGCGCTCA ACTGGCTGGG AGTCACTGTG GAGAGTGGGG TCCGGAGGCT GCTAGAGACA 
CCCTGTGTCC GGGAGACTTT GGCCCGGCAC CTCTCCCCAC AAGCCCACCA TCGACTGCAC 
GCTCTGCTTG CCGCCTGCTC TACCTCCATG CTCATCCTCT TCAACCTGGT GTTCCTCGGG 
GGAATTCAAG TTGGGAAAAC CTACTGGAAC AGGATCTTCC TGCAAGGGTG GCCATGGGTG 
ACTCTGTCTG TCCTGGGATT CCTGTACTGC TATTCTCACG TGGACATTGC TTGGGCTCAG 
ACATACACAG TTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hhat ( XM_017320221.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175710.1 Mus musculus hedgehog acyltransferase (Hhat), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu77108
Clone ID Related Accession (Same CDS sequence)	XM_017320221.2 , XM_017320221.1
Accession Version	XM_017320221.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein-cysteine N-palmitoyltransferase HHAT isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCAGC CAGAGCTCAA CTCTCTGCAG CACAGCCTCT GCATTGTAGC CAAGGGCCTG 
GGTCGTCTGC TCTGCTGGTG GTGGCTGGCT GAACTGATGG TACACCTCAT GTACATGCAT 
GCCCTCTACA GCAGCGCCCC CCTCCTGGAG AGCGTTTCCT GCTGGACCTT AGGAGGACTG 
GCGCTGGCCC AAGTGCTCTT CTTCTATGTG AAGTACCTGG TGCTCTTTGG TGTCCCGGCT 
CTGCTCATGC GCCTGGATGG ACTCACGCCA CCACCTCTTC CCCGCTGCGT GAGCACCATG 
TTCAGTTTTA CCGGGATGTG GAGGTATTTT GATGTTGGAC TACACAATTT CTTAATCAGG 
TATGTGTACA TCCCACTGGG CGGGTCCCAG CATGGCCTGT TGGGGACACT GCTTTCTACT 
GCAACAACGT TTGCGTTCGT GAGCTACTGG CATGGTAGCT ATGAGGACCT CTGGTGCTGG 
GCAGCGCTCA ACTGGCTGGG AGTCACTGTG GAGAGTGGGG TCCGGAGGCT GCTAGAGACA 
CCCTGTGTCC GGGAGACTTT GGCCCGGCAC CTCTCCCCAC AAGCCCACCA TCGACTGCAC 
GCTCTGCTTG CCGCCTGCTC TACCTCCATG CTCATCCTCT TCAACCTGGT GTTCCTCGGG 
GGAATTCAAG TTGGGAAAAC CTACTGGAAC AGGATCTTCC TGCAAGGGTG GCCATGGGTG 
ACTCTGTCTG TCCTGGGATT CCTGTACTGC TATTCTCACG TGGACATTGC TTGGGCTCAG 
ACATACACAG TTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175710.1
CDS	81..878
Translation	MQQPELNSLQHSLCIVAKGLGRLLCWWWLAELMVHLMYMHALYSSAPLLESVSCWTLGGLALAQVLFFYVKYLVLFGVPALLMRLDGLTPPPLPRCVSTMFSFTGMWRYFDVGLHNFLIRYVYIPLGGSQHGLLGTLLSTATTFAFVSYWHGSYEDLWCWAALNWLGVTVESGVRRLLETPCVRETLARHLSPQAHHRLHALLAACSTSMLILFNLVFLGGIQVGKTYWNRIFLQGWPWVTLSVLGFLYCYSHVDIAWAQTYTVL

Target ORF information:

RefSeq Version	XM_017320221.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus hedgehog acyltransferase (Hhat), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320221.1

ORF Insert Sequence:

ATGCAGCAGC CAGAGCTCAA CTCTCTGCAG CACAGCCTCT GCATTGTAGC CAAGGGCCTG 
GGTCGTCTGC TCTGCTGGTG GTGGCTGGCT GAACTGATGG TACACCTCAT GTACATGCAT 
GCCCTCTACA GCAGCGCCCC CCTCCTGGAG AGCGTTTCCT GCTGGACCTT AGGAGGACTG 
GCGCTGGCCC AAGTGCTCTT CTTCTATGTG AAGTACCTGG TGCTCTTTGG TGTCCCGGCT 
CTGCTCATGC GCCTGGATGG ACTCACGCCA CCACCTCTTC CCCGCTGCGT GAGCACCATG 
TTCAGTTTTA CCGGGATGTG GAGGTATTTT GATGTTGGAC TACACAATTT CTTAATCAGG 
TATGTGTACA TCCCACTGGG CGGGTCCCAG CATGGCCTGT TGGGGACACT GCTTTCTACT 
GCAACAACGT TTGCGTTCGT GAGCTACTGG CATGGTAGCT ATGAGGACCT CTGGTGCTGG 
GCAGCGCTCA ACTGGCTGGG AGTCACTGTG GAGAGTGGGG TCCGGAGGCT GCTAGAGACA 
CCCTGTGTCC GGGAGACTTT GGCCCGGCAC CTCTCCCCAC AAGCCCACCA TCGACTGCAC 
GCTCTGCTTG CCGCCTGCTC TACCTCCATG CTCATCCTCT TCAACCTGGT GTTCCTCGGG 
GGAATTCAAG TTGGGAAAAC CTACTGGAAC AGGATCTTCC TGCAAGGGTG GCCATGGGTG 
ACTCTGTCTG TCCTGGGATT CCTGTACTGC TATTCTCACG TGGACATTGC TTGGGCTCAG 
ACATACACAG TTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hhat ( XM_011238934.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237236.1 Mus musculus hedgehog acyltransferase (Hhat), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu73314
Clone ID Related Accession (Same CDS sequence)	XM_011238934.3 , XM_011238934.2
Accession Version	XM_011238934.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein-cysteine N-palmitoyltransferase HHAT isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238934.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCTG AGAGGCAGCT CAGTGGCAGA GCTATATCTG TGCCTAAGTG GGCGGCGGCC 
CTGGGTTCAA TCCCTTACAC TGGAGACTGG GGTTCCACAG TCCTTAGGGA CAGAGGCAGG 
ATCTGGCCCT GTGACTGTGT ATTTCTGACC TGCTATCTCA GGCTGATCAG GGAACAAGAA 
CGGGAGCTAG ATTGTTTTCC TGGGGGTTGG GGCGAGGAGT CCCAGCTGAC TGGGAAGTCA 
GAAGTGAAGG GATGCTCATC GCCGAGCCAC ACTGGCACTG TGGTGGCCCT CCCATTCGCA 
CACAGACCCT GGATTGTCAT GGTCTATGGA ATGTGGGCCT GCTGGTGTGT GCTGGGGGCG 
CCAGGCGTGG TCATGGTCCT TCTCCACAGC ACCATCGCGT TCTGCGTGGC TCAGTTCCGA 
TCCGTGCTCC TGTCCTGGCT GTGTTCTCTC CTTCTGCTCT CCACCCTAAG GCTGCAAAGT 
GTGGAGGAAG TTAAGAGAAG GTGGTACAAG ACAGAGAACG AGTATTACCT GCTCCAGTTT 
ACGCTCACTG TTCGCTGCCT GTACTACACC AGCTTCAGCC TGGAGCTGTG CCGGCAGCCT 
CCGTCTGCAC AACCTACTCC ATCTGCGCAA GGTGCCTCCC ATTCCTACCC GTGGCTGCTG 
ACCTACGTCT TCTATTATCC CGTCTTCCAC AACGGGCCCA TCCTTAACTT CCCGGAGTTC 
TTCAGACAGA TGCAGCAGCC AGAGCTCAAC TCTCTGCAGC ACAGCCTCTG CATTGTAGCC 
AAGGGCCTGG GTCGTCTGCT CTGCTGGTGG TGGCTGGCTG AACTGATGGT ACACCTCATG 
TACATGCATG CCCTCTACAG CAGCGCCCCC CTCCTGGAGA GCGTTTCCTG CTGGACCTTA 
GGAGGACTGG CGCTGGCCCA AGTGCTCTTC TTCTATGTGA AGTACCTGGT GCTCTTTGGT 
GTCCCGGCTC TGCTCATGCG CCTGGATGGA CTCACGCCAC CACCTCTTCC CCGCTGCGTG 
AGCACCATGT TCAGTTTTAC CGGGATGTGG AGGTATTTTG ATGTTGGACT ACACAATTTC 
TTAATCAGGT ATGTGTACAT CCCACTGGGC GGGTCCCAGC ATGGCCTGTT GGGGACACTG 
CTTTCTACTG CAACAACGTT TGCGTTCGTG AGCTACTGGC ATGGTAGCTA TGAGGACCTC 
TGGTGCTGGG CAGCGCTCAA CTGGCTGGGA GTCACTGTGG AGAGTGGGGT CCGGAGGCTG 
CTAGAGACAC CCTGTGTCCG GGAGACTTTG GCCCGGCACC TCTCCCCACA AGCCCACCAT 
CGACTGCACG CTCTGCTTGC CGCCTGCTCT ACCTCCATGC TCATCCTCTT CAACCTGGTG 
TTCCTCGGGG GAATTCAAGT TGGGAAAACC TACTGGAACA GGATCTTCCT GCAAGGGTGG 
CCATGGGTGA CTCTGTCTGT CCTGGGATTC CTGTACTGCT ATTCTCACGT GGACATTGCT 
TGGGCTCAGA CATACACAGT TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237236.1
CDS	99..1625
Translation	MQAERQLSGRAISVPKWAAALGSIPYTGDWGSTVLRDRGRIWPCDCVFLTCYLRLIREQERELDCFPGGWGEESQLTGKSEVKGCSSPSHTGTVVALPFAHRPWIVMVYGMWACWCVLGAPGVVMVLLHSTIAFCVAQFRSVLLSWLCSLLLLSTLRLQSVEEVKRRWYKTENEYYLLQFTLTVRCLYYTSFSLELCRQPPSAQPTPSAQGASHSYPWLLTYVFYYPVFHNGPILNFPEFFRQMQQPELNSLQHSLCIVAKGLGRLLCWWWLAELMVHLMYMHALYSSAPLLESVSCWTLGGLALAQVLFFYVKYLVLFGVPALLMRLDGLTPPPLPRCVSTMFSFTGMWRYFDVGLHNFLIRYVYIPLGGSQHGLLGTLLSTATTFAFVSYWHGSYEDLWCWAALNWLGVTVESGVRRLLETPCVRETLARHLSPQAHHRLHALLAACSTSMLILFNLVFLGGIQVGKTYWNRIFLQGWPWVTLSVLGFLYCYSHVDIAWAQTYTVL

Target ORF information:

RefSeq Version	XM_011238934.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus hedgehog acyltransferase (Hhat), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238934.3

ORF Insert Sequence:

ATGCAGGCTG AGAGGCAGCT CAGTGGCAGA GCTATATCTG TGCCTAAGTG GGCGGCGGCC 
CTGGGTTCAA TCCCTTACAC TGGAGACTGG GGTTCCACAG TCCTTAGGGA CAGAGGCAGG 
ATCTGGCCCT GTGACTGTGT ATTTCTGACC TGCTATCTCA GGCTGATCAG GGAACAAGAA 
CGGGAGCTAG ATTGTTTTCC TGGGGGTTGG GGCGAGGAGT CCCAGCTGAC TGGGAAGTCA 
GAAGTGAAGG GATGCTCATC GCCGAGCCAC ACTGGCACTG TGGTGGCCCT CCCATTCGCA 
CACAGACCCT GGATTGTCAT GGTCTATGGA ATGTGGGCCT GCTGGTGTGT GCTGGGGGCG 
CCAGGCGTGG TCATGGTCCT TCTCCACAGC ACCATCGCGT TCTGCGTGGC TCAGTTCCGA 
TCCGTGCTCC TGTCCTGGCT GTGTTCTCTC CTTCTGCTCT CCACCCTAAG GCTGCAAAGT 
GTGGAGGAAG TTAAGAGAAG GTGGTACAAG ACAGAGAACG AGTATTACCT GCTCCAGTTT 
ACGCTCACTG TTCGCTGCCT GTACTACACC AGCTTCAGCC TGGAGCTGTG CCGGCAGCCT 
CCGTCTGCAC AACCTACTCC ATCTGCGCAA GGTGCCTCCC ATTCCTACCC GTGGCTGCTG 
ACCTACGTCT TCTATTATCC CGTCTTCCAC AACGGGCCCA TCCTTAACTT CCCGGAGTTC 
TTCAGACAGA TGCAGCAGCC AGAGCTCAAC TCTCTGCAGC ACAGCCTCTG CATTGTAGCC 
AAGGGCCTGG GTCGTCTGCT CTGCTGGTGG TGGCTGGCTG AACTGATGGT ACACCTCATG 
TACATGCATG CCCTCTACAG CAGCGCCCCC CTCCTGGAGA GCGTTTCCTG CTGGACCTTA 
GGAGGACTGG CGCTGGCCCA AGTGCTCTTC TTCTATGTGA AGTACCTGGT GCTCTTTGGT 
GTCCCGGCTC TGCTCATGCG CCTGGATGGA CTCACGCCAC CACCTCTTCC CCGCTGCGTG 
AGCACCATGT TCAGTTTTAC CGGGATGTGG AGGTATTTTG ATGTTGGACT ACACAATTTC 
TTAATCAGGT ATGTGTACAT CCCACTGGGC GGGTCCCAGC ATGGCCTGTT GGGGACACTG 
CTTTCTACTG CAACAACGTT TGCGTTCGTG AGCTACTGGC ATGGTAGCTA TGAGGACCTC 
TGGTGCTGGG CAGCGCTCAA CTGGCTGGGA GTCACTGTGG AGAGTGGGGT CCGGAGGCTG 
CTAGAGACAC CCTGTGTCCG GGAGACTTTG GCCCGGCACC TCTCCCCACA AGCCCACCAT 
CGACTGCACG CTCTGCTTGC CGCCTGCTCT ACCTCCATGC TCATCCTCTT CAACCTGGTG 
TTCCTCGGGG GAATTCAAGT TGGGAAAACC TACTGGAACA GGATCTTCCT GCAAGGGTGG 
CCATGGGTGA CTCTGTCTGT CCTGGGATTC CTGTACTGCT ATTCTCACGT GGACATTGCT 
TGGGCTCAGA CATACACAGT TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hydronephrosis in the Wnt5a-ablated kidney is caused by an abnormal ureter-bladder connection.
Differentiation; research in biological diversity941-7(2017 Mar - Apr)
Yun K,Perantoni AO

Ventral neural patterning in the absence of a Shh activity gradient from the floorplate.
Developmental dynamics : an official publication of the American Association of Anatomists247(1)170-184(2018 Jan)
Iulianella A,Sakai D,Kurosaka H,Trainor PA

Cranial nerve development requires co-ordinated Shh and canonical Wnt signaling.
PloS one10(3)e0120821(2015)
Kurosaka H,Trainor PA,Leroux-Berger M,Iulianella A

Loss of function mutation in the palmitoyl-transferase HHAT leads to syndromic 46,XY disorder of sex development by impeding Hedgehog protein palmitoylation and signaling.
PLoS genetics10(5)e1004340(2014 May)
Callier P,Calvel P,Matevossian A,Makrythanasis P,Bernard P,Kurosaka H,Vannier A,Thauvin-Robinet C,Borel C,Mazaud-Guittot S,Rolland A,Desdoits-Lethimonier C,Guipponi M,Zimmermann C,St?vant I,Kuhne F,Conne B,Santoni F,Lambert S,Huet F,Mugneret F,Jaruzelska J,Faivre L,Wilhelm D,J?gou B,Trainor PA,Resh MD,Antonarakis SE,Nef S

Disrupting hedgehog and WNT signaling interactions promotes cleft lip pathogenesis.
The Journal of clinical investigation124(4)1660-71(2014 Apr)
Kurosaka H,Iulianella A,Williams T,Trainor PA

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Hhat cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム