Zfp281 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Zfp281
Entrez Gene ID	226442
Full Name	zinc finger protein 281
Synonyms	Znf281
General protein information	Preferred Names zinc finger protein 281 Names zinc finger protein 281
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 E4

mRNA and Protein(s)

mRNA	Protein	Name
NM_177643.4	NP_808311.1	zinc finger protein 281
NM_001160251.1	NP_001153723.1	zinc finger protein 281

Pathways from BioSystems

Homologies

	ZNF281	XP_001110006.2
Mus musculus (house mouse)	Zfp281	NP_001153723.1
Danio rerio (zebrafish)	zgc:113026	NP_001012505.2
Danio rerio (zebrafish)	LOC100005963	XP_001922388.3
Xenopus tropicalis (tropical clawed frog)	znf281	XP_002935748.1
Homo sapiens (human)	ZNF281	NP_036614.1
Pan troglodytes (chimpanzee)	ZNF281	XP_525015.3
Bos taurus (cattle)	ZNF281	XP_002694282.2
Rattus norvegicus (Norway rat)	Zfp281	NP_001012030.1
Gallus gallus (chicken)	ZNF281	XP_004943166.1
Canis lupus familiaris (dog)	ZNF281	XP_005622332.1

Gene Ontology
Bibliography

Related articles in PubMed

Striking a balance: regulation of transposable elements by Zfp281 and Mll2 in mouse embryonic stem cells.
Dai Q, Shen Y, Wang Y, Wang X, Francisco JC, Luo Z, Lin C
Nucleic acids research45(21)12301-12310(2017 Dec)

Zfp281 is essential for mouse epiblast maturation through transcriptional and epigenetic control of Nodal signaling.
Huang X, Balmer S, Yang F, Fidalgo M, Li D, Guallar D, Hadjantonakis AK, Wang J
eLife6(2017 Nov)

A permissive chromatin state regulated by ZFP281-AFF3 in controlling the imprinted Meg3 polycistron.
Wang Y, Shen Y, Dai Q, Yang Q, Zhang Y, Wang X, Xie W, Luo Z, Lin C
Nucleic acids research45(3)1177-1185(2017 02)

Zfp281 Coordinates Opposing Functions of Tet1 and Tet2 in Pluripotent States.
Fidalgo M, Huang X, Guallar D, Sanchez-Priego C, Valdes VJ, Saunders A, Ding J, Wu WS, Clavel C, Wang J
Cell stem cell19(3)355-69(2016 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

an important role of Zfp281 in restricting the expression of F type of young L1s in mouse embryonic stem cells.
Title: Striking a balance: regulation of transposable elements by Zfp281 and Mll2 in mouse embryonic stem cells.

AFF3 is associated with the Kruppel-like zinc finger protein ZFP281 in embryonic stem (ES) cells. ZFP281 recruits AFF3 to the Meg3 enhancer within the imprinted Dlk1-Dio3 locus, thus regulating the allele-specific expression of the Meg3 polycistron.
Title: A permissive chromatin state regulated by ZFP281-AFF3 in controlling the imprinted Meg3 polycistron.

Zfp281 interacts with Tet1, but not Tet2, and its direct transcriptional target, miR-302/367, to negatively regulate Tet2 expression to establish and maintain primed pluripotency.
Title: Zfp281 Coordinates Opposing Functions of Tet1 and Tet2 in Pluripotent States.

involvement of ZNF281 in the cellular response to genotoxic stress through the control exercised on the expression of genes that act in different repair mechanisms
Title: ZNF281 contributes to the DNA damage response by controlling the expression of XRCC2 and XRCC4.

Zpf281 mediates Nanog autorepression through recruitment of the NuRD complex and hibits somatic cell reprogramming.
Title: Zfp281 mediates Nanog autorepression through recruitment of the NuRD complex and inhibits somatic cell reprogramming.

The following Zfp281 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Zfp281 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu06591	NM_001160251.1 Latest version!	Mus musculus zinc finger protein 281 (Zfp281), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OMu06591	NM_177643.4 Latest version!	Mus musculus zinc finger protein 281 (Zfp281), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Zfp281 ( NM_177643.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_808311.1 Mus musculus zinc finger protein 281 (Zfp281), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu06591
Clone ID Related Accession (Same CDS sequence)	NM_177643.4 , NM_001160251.1
Accession Version	NM_177643.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Mus musculus(house mouse)
Product	zinc finger protein 281
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AV581959.1, AK142218.1 and AC126606.16. On May 16, 2009 this sequence version replaced NM_177643.3. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AI785752.1, BC003243.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAATCG GCAGCGGGTT CCTTAGCGGC GGCGGCGGTC CCAGCAGTAG CGGTGGTAGC 
GGCTCCGGCG GCAGCTCCGG CAGCGCCAGC GGCGGCAGCG GCGGCGGCAG GAGAGCAGAG 
ATGGAGCCCA CCTTCCCCCA GAGTATGGTT ATGTTCAACC ACCGGCTTCC CCCGGTCACC 
AGCTTCACCC GGCCGGCGGG GACGGCCGCC CCTCCCCCGC AGTGCGTGTT ATCCTCCTCT 
ACCTCCGCAG CCCCGGCCGC TGAGCCCCCC CCTCCGCCAG CCCCGGACAT GACTTTCAAG 
AAGGAGCCGG CGGCGTCAGC CGCGGCCTTC CCTTCGCAGA GGACCTCCTG GGGATTCTTG 
CAGTCCTTGG TGAGCATCAA GCAGGAGAAA CCTGCGGATC CCGAGGAGCA GCCGTCCCAC 
CACCACCATC ACCACCACCA CTATGGGGGG CTGTTCGCTG GGGCTGAAGA GCGATCACCA 
GGCCTAGGAG GAGGGGAAGG GGGGAGCCAC GGCGTCATCC AAGACCTCAG TCTTCTGCAC 
CAGCATGCCC AGCAGCAACC GGCCCAGCAC CACCGCGATG TATTACTGAG CAGCGGTAGC 
AGGACTGATG AGCACGGCAA CCAGGAGCCA AAGCAGGACG CTAACGTCAA AAAGGCAAAG 
AGGCCAAAGC CAGAATCTCA GGGAATCAAA GCCAAGAGGA AGCCAAGTGC ATCTTCCAAA 
CCTTTGGTTG GAGAGGGAGA AGGTGCCGTC CTGTCCCCAA GTCAGAAACC TCATATCTGT 
GATCACTGTA GTGCTGCTTT CAGGAGCTCC TACCACCTGC GGAGACATGT CCTCATCCAC 
ACGGGCGAAA GGCCTTTCCA GTGCAGCCAG TGTAGTATGG GTTTCATCCA GAAATACCTA 
CTGCAGAGAC ACGAGAAAAT TCACAGCAGA GAGAAACCCT TTGGGTGCGA TCAGTGCAGC 
ATGAAGTTTA TTCAGAAGTA CCATATGGAG AGACACAAGA GGACACATAG TGGAGAAAAG 
CCATACAAGT GTGACACTTG CCAACAGTAT TTTTCAAGGA CTGACAGATT GTTGAAGCAC 
AGGCGCACGT GTGGTGAAGC CATAGCAAAA GGAGCCGCTA GTGCAGAACC TGGGTCATCA 
AACCATAACA GTATGGGTAA CCTGGCTGTG TTGTCTCAGG GAAATACAAG TTCCTCAAGG 
AGAAAGTCGA AGTCGAAAAG CATAGCTATT GAAAATAAGG AACACAAGAC TGGCAAAACA 
AATGAATCAC AAATGTCAAA CAATATAAAC ATGCAGAGTT ATTCAGTAGA AATGCCTACT 
GTGTCTACCA GTGGGAGCAT AATTGGCACT GGAATAGATG AACTGCAGAA AAGGGTGCCA 
AAATTGATCT TTAAGAAAGG AAGCAGAAAG AATGCAGATA AAAGCTACCT TAATTTTGTG 
TCACCGTTAC CAGACGTAGT TGGGCAGAAA TCCTTGTCTG GTAAGCCAGG TGGCTCACTT 
GGCATCGTAT CGAATAATAG TGTGGAGACC ATTAGTCTTC TCCAAAGTAC AAGTGGCAAG 
CAAGGCCCGA TAAGTAGTAA TTATGATGAT GCCATGCAGT TTTCAAAGAA GAGAAGATAC 
CTACCAACTG CCAGCAGCAA CAGTGCCTTT TCTATCAATG TAGGACACAT GGTCTCCCAG 
CAGTCAGTCA TTCAGTCTGC AGGGGTCAGT GTTTTGGACA ATGAGGCGCC ATTGTCACTT 
ATTGACTCCT CAGCTCTAAA TGCTGAAATT AAGTCTTGTC ACGACAAGTC TGGAATTCCT 
GATGAGGTTT TACAAAGTAT TTTGGATCAG TACTCTGGCA AATCAGAAAC ACAGAAGGAG 
GATCCTTTCA ATTTAACAGA ACCACGAGTG GATTTACACA CCTCAGGAGA ACACTCTGAA 
TTGGTTCAAG AAGAAAATTT GAGCCCAGGC ACCCAAACAC CTTCAAATGA TAAAACAAGC 
ATGTTGCAAG AATACTCCAA ATACCTCCAA CAGGCTTTCG AAAAATCCAC TAATGCAGGT 
TTTACTCTTG GACACGGTTT CCAATTTGTC AGCTTGTCTT CACCTCTCCA CAACCACACT 
TTGTTTCCAG AAAAACAGAT ATACACTACA TCTCCTTTGG AGTGTGGTTT TGGCCAGTCT 
GTTACCTCAG TGCTGCCATC TTCGTTGCCA AAGCCTCCTT TTGGGATGTT GTTTGGATCT 
CAACCAGGTC TTTATTTATC TGCTTTGGAT GCTACACATC AGCAGTTGAC ACCTTCCCAG 
GAGCTGGATG ATCTGATAGA TTCTCAGAAG AACTTAGAGA CATCATCAGC CTTCCAGTCC 
TCATCTCAGA AATTGACTAG CCAGAAGGAA CAACAGAAAA ATTTAGAGTC CTCAACGAGC 
TTTCAGATTC CATCTCAGGA GTTAGCTAGC CAGATTGATC CTCAGAAAGA CATAGAGCCT 
AGAACAACGT ACCAGATTGA GAACTTTGCA CAAGCATTCG GTTCTCAGTT TAAGTCGGGC 
AGCAGGGTGC CAATGACCTT TATCACTAAC TCTAATGGAG AAGTGGACCA TAGAGTAAGG 
ACTTCAGTGT CAGATTTCTC AGGGTATACA AATATGATGT CTGATGTAAG TGAGCCATGT 
AGTACAAGAG TAAAGACACC CACCAGCCAG AGTTACAGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808311.1
CDS	166..2847
Translation	MKIGSGFLSGGGGPSSSGGSGSGGSSGSASGGSGGGRRAEMEPTFPQSMVMFNHRLPPVTSFTRPAGTAAPPPQCVLSSSTSAAPAAEPPPPPAPDMTFKKEPAASAAAFPSQRTSWGFLQSLVSIKQEKPADPEEQPSHHHHHHHHYGGLFAGAEERSPGLGGGEGGSHGVIQDLSLLHQHAQQQPAQHHRDVLLSSGSRTDEHGNQEPKQDANVKKAKRPKPESQGIKAKRKPSASSKPLVGEGEGAVLSPSQKPHICDHCSAAFRSSYHLRRHVLIHTGERPFQCSQCSMGFIQKYLLQRHEKIHSREKPFGCDQCSMKFIQKYHMERHKRTHSGEKPYKCDTCQQYFSRTDRLLKHRRTCGEAIAKGAASAEPGSSNHNSMGNLAVLSQGNTSSSRRKSKSKSIAIENKEHKTGKTNESQMSNNINMQSYSVEMPTVSTSGSIIGTGIDELQKRVPKLIFKKGSRKNADKSYLNFVSPLPDVVGQKSLSGKPGGSLGIVSNNSVETISLLQSTSGKQGPISSNYDDAMQFSKKRRYLPTASSNSAFSINVGHMVSQQSVIQSAGVSVLDNEAPLSLIDSSALNAEIKSCHDKSGIPDEVLQSILDQYSGKSETQKEDPFNLTEPRVDLHTSGEHSELVQEENLSPGTQTPSNDKTSMLQEYSKYLQQAFEKSTNAGFTLGHGFQFVSLSSPLHNHTLFPEKQIYTTSPLECGFGQSVTSVLPSSLPKPPFGMLFGSQPGLYLSALDATHQQLTPSQELDDLIDSQKNLETSSAFQSSSQKLTSQKEQQKNLESSTSFQIPSQELASQIDPQKDIEPRTTYQIENFAQAFGSQFKSGSRVPMTFITNSNGEVDHRVRTSVSDFSGYTNMMSDVSEPCSTRVKTPTSQSYR

Target ORF information:

RefSeq Version	NM_177643.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus zinc finger protein 281 (Zfp281), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177643.4

ORF Insert Sequence:

ATGAAAATCG GCAGCGGGTT CCTTAGCGGC GGCGGCGGTC CCAGCAGTAG CGGTGGTAGC 
GGCTCCGGCG GCAGCTCCGG CAGCGCCAGC GGCGGCAGCG GCGGCGGCAG GAGAGCAGAG 
ATGGAGCCCA CCTTCCCCCA GAGTATGGTT ATGTTCAACC ACCGGCTTCC CCCGGTCACC 
AGCTTCACCC GGCCGGCGGG GACGGCCGCC CCTCCCCCGC AGTGCGTGTT ATCCTCCTCT 
ACCTCCGCAG CCCCGGCCGC TGAGCCCCCC CCTCCGCCAG CCCCGGACAT GACTTTCAAG 
AAGGAGCCGG CGGCGTCAGC CGCGGCCTTC CCTTCGCAGA GGACCTCCTG GGGATTCTTG 
CAGTCCTTGG TGAGCATCAA GCAGGAGAAA CCTGCGGATC CCGAGGAGCA GCCGTCCCAC 
CACCACCATC ACCACCACCA CTATGGGGGG CTGTTCGCTG GGGCTGAAGA GCGATCACCA 
GGCCTAGGAG GAGGGGAAGG GGGGAGCCAC GGCGTCATCC AAGACCTCAG TCTTCTGCAC 
CAGCATGCCC AGCAGCAACC GGCCCAGCAC CACCGCGATG TATTACTGAG CAGCGGTAGC 
AGGACTGATG AGCACGGCAA CCAGGAGCCA AAGCAGGACG CTAACGTCAA AAAGGCAAAG 
AGGCCAAAGC CAGAATCTCA GGGAATCAAA GCCAAGAGGA AGCCAAGTGC ATCTTCCAAA 
CCTTTGGTTG GAGAGGGAGA AGGTGCCGTC CTGTCCCCAA GTCAGAAACC TCATATCTGT 
GATCACTGTA GTGCTGCTTT CAGGAGCTCC TACCACCTGC GGAGACATGT CCTCATCCAC 
ACGGGCGAAA GGCCTTTCCA GTGCAGCCAG TGTAGTATGG GTTTCATCCA GAAATACCTA 
CTGCAGAGAC ACGAGAAAAT TCACAGCAGA GAGAAACCCT TTGGGTGCGA TCAGTGCAGC 
ATGAAGTTTA TTCAGAAGTA CCATATGGAG AGACACAAGA GGACACATAG TGGAGAAAAG 
CCATACAAGT GTGACACTTG CCAACAGTAT TTTTCAAGGA CTGACAGATT GTTGAAGCAC 
AGGCGCACGT GTGGTGAAGC CATAGCAAAA GGAGCCGCTA GTGCAGAACC TGGGTCATCA 
AACCATAACA GTATGGGTAA CCTGGCTGTG TTGTCTCAGG GAAATACAAG TTCCTCAAGG 
AGAAAGTCGA AGTCGAAAAG CATAGCTATT GAAAATAAGG AACACAAGAC TGGCAAAACA 
AATGAATCAC AAATGTCAAA CAATATAAAC ATGCAGAGTT ATTCAGTAGA AATGCCTACT 
GTGTCTACCA GTGGGAGCAT AATTGGCACT GGAATAGATG AACTGCAGAA AAGGGTGCCA 
AAATTGATCT TTAAGAAAGG AAGCAGAAAG AATGCAGATA AAAGCTACCT TAATTTTGTG 
TCACCGTTAC CAGACGTAGT TGGGCAGAAA TCCTTGTCTG GTAAGCCAGG TGGCTCACTT 
GGCATCGTAT CGAATAATAG TGTGGAGACC ATTAGTCTTC TCCAAAGTAC AAGTGGCAAG 
CAAGGCCCGA TAAGTAGTAA TTATGATGAT GCCATGCAGT TTTCAAAGAA GAGAAGATAC 
CTACCAACTG CCAGCAGCAA CAGTGCCTTT TCTATCAATG TAGGACACAT GGTCTCCCAG 
CAGTCAGTCA TTCAGTCTGC AGGGGTCAGT GTTTTGGACA ATGAGGCGCC ATTGTCACTT 
ATTGACTCCT CAGCTCTAAA TGCTGAAATT AAGTCTTGTC ACGACAAGTC TGGAATTCCT 
GATGAGGTTT TACAAAGTAT TTTGGATCAG TACTCTGGCA AATCAGAAAC ACAGAAGGAG 
GATCCTTTCA ATTTAACAGA ACCACGAGTG GATTTACACA CCTCAGGAGA ACACTCTGAA 
TTGGTTCAAG AAGAAAATTT GAGCCCAGGC ACCCAAACAC CTTCAAATGA TAAAACAAGC 
ATGTTGCAAG AATACTCCAA ATACCTCCAA CAGGCTTTCG AAAAATCCAC TAATGCAGGT 
TTTACTCTTG GACACGGTTT CCAATTTGTC AGCTTGTCTT CACCTCTCCA CAACCACACT 
TTGTTTCCAG AAAAACAGAT ATACACTACA TCTCCTTTGG AGTGTGGTTT TGGCCAGTCT 
GTTACCTCAG TGCTGCCATC TTCGTTGCCA AAGCCTCCTT TTGGGATGTT GTTTGGATCT 
CAACCAGGTC TTTATTTATC TGCTTTGGAT GCTACACATC AGCAGTTGAC ACCTTCCCAG 
GAGCTGGATG ATCTGATAGA TTCTCAGAAG AACTTAGAGA CATCATCAGC CTTCCAGTCC 
TCATCTCAGA AATTGACTAG CCAGAAGGAA CAACAGAAAA ATTTAGAGTC CTCAACGAGC 
TTTCAGATTC CATCTCAGGA GTTAGCTAGC CAGATTGATC CTCAGAAAGA CATAGAGCCT 
AGAACAACGT ACCAGATTGA GAACTTTGCA CAAGCATTCG GTTCTCAGTT TAAGTCGGGC 
AGCAGGGTGC CAATGACCTT TATCACTAAC TCTAATGGAG AAGTGGACCA TAGAGTAAGG 
ACTTCAGTGT CAGATTTCTC AGGGTATACA AATATGATGT CTGATGTAAG TGAGCCATGT 
AGTACAAGAG TAAAGACACC CACCAGCCAG AGTTACAGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Zfp281 ( NM_001160251.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001153723.1 Mus musculus zinc finger protein 281 (Zfp281), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu06591
Clone ID Related Accession (Same CDS sequence)	NM_177643.4 , NM_001160251.1
Accession Version	NM_001160251.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Mus musculus(house mouse)
Product	zinc finger protein 281
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AV581959.1, BC062153.1 and AC126606.16. Transcript Variant: This variant (1) represents the longer transcript. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC062153.1, BQ925711.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAATCG GCAGCGGGTT CCTTAGCGGC GGCGGCGGTC CCAGCAGTAG CGGTGGTAGC 
GGCTCCGGCG GCAGCTCCGG CAGCGCCAGC GGCGGCAGCG GCGGCGGCAG GAGAGCAGAG 
ATGGAGCCCA CCTTCCCCCA GAGTATGGTT ATGTTCAACC ACCGGCTTCC CCCGGTCACC 
AGCTTCACCC GGCCGGCGGG GACGGCCGCC CCTCCCCCGC AGTGCGTGTT ATCCTCCTCT 
ACCTCCGCAG CCCCGGCCGC TGAGCCCCCC CCTCCGCCAG CCCCGGACAT GACTTTCAAG 
AAGGAGCCGG CGGCGTCAGC CGCGGCCTTC CCTTCGCAGA GGACCTCCTG GGGATTCTTG 
CAGTCCTTGG TGAGCATCAA GCAGGAGAAA CCTGCGGATC CCGAGGAGCA GCCGTCCCAC 
CACCACCATC ACCACCACCA CTATGGGGGG CTGTTCGCTG GGGCTGAAGA GCGATCACCA 
GGCCTAGGAG GAGGGGAAGG GGGGAGCCAC GGCGTCATCC AAGACCTCAG TCTTCTGCAC 
CAGCATGCCC AGCAGCAACC GGCCCAGCAC CACCGCGATG TATTACTGAG CAGCGGTAGC 
AGGACTGATG AGCACGGCAA CCAGGAGCCA AAGCAGGACG CTAACGTCAA AAAGGCAAAG 
AGGCCAAAGC CAGAATCTCA GGGAATCAAA GCCAAGAGGA AGCCAAGTGC ATCTTCCAAA 
CCTTTGGTTG GAGAGGGAGA AGGTGCCGTC CTGTCCCCAA GTCAGAAACC TCATATCTGT 
GATCACTGTA GTGCTGCTTT CAGGAGCTCC TACCACCTGC GGAGACATGT CCTCATCCAC 
ACGGGCGAAA GGCCTTTCCA GTGCAGCCAG TGTAGTATGG GTTTCATCCA GAAATACCTA 
CTGCAGAGAC ACGAGAAAAT TCACAGCAGA GAGAAACCCT TTGGGTGCGA TCAGTGCAGC 
ATGAAGTTTA TTCAGAAGTA CCATATGGAG AGACACAAGA GGACACATAG TGGAGAAAAG 
CCATACAAGT GTGACACTTG CCAACAGTAT TTTTCAAGGA CTGACAGATT GTTGAAGCAC 
AGGCGCACGT GTGGTGAAGC CATAGCAAAA GGAGCCGCTA GTGCAGAACC TGGGTCATCA 
AACCATAACA GTATGGGTAA CCTGGCTGTG TTGTCTCAGG GAAATACAAG TTCCTCAAGG 
AGAAAGTCGA AGTCGAAAAG CATAGCTATT GAAAATAAGG AACACAAGAC TGGCAAAACA 
AATGAATCAC AAATGTCAAA CAATATAAAC ATGCAGAGTT ATTCAGTAGA AATGCCTACT 
GTGTCTACCA GTGGGAGCAT AATTGGCACT GGAATAGATG AACTGCAGAA AAGGGTGCCA 
AAATTGATCT TTAAGAAAGG AAGCAGAAAG AATGCAGATA AAAGCTACCT TAATTTTGTG 
TCACCGTTAC CAGACGTAGT TGGGCAGAAA TCCTTGTCTG GTAAGCCAGG TGGCTCACTT 
GGCATCGTAT CGAATAATAG TGTGGAGACC ATTAGTCTTC TCCAAAGTAC AAGTGGCAAG 
CAAGGCCCGA TAAGTAGTAA TTATGATGAT GCCATGCAGT TTTCAAAGAA GAGAAGATAC 
CTACCAACTG CCAGCAGCAA CAGTGCCTTT TCTATCAATG TAGGACACAT GGTCTCCCAG 
CAGTCAGTCA TTCAGTCTGC AGGGGTCAGT GTTTTGGACA ATGAGGCGCC ATTGTCACTT 
ATTGACTCCT CAGCTCTAAA TGCTGAAATT AAGTCTTGTC ACGACAAGTC TGGAATTCCT 
GATGAGGTTT TACAAAGTAT TTTGGATCAG TACTCTGGCA AATCAGAAAC ACAGAAGGAG 
GATCCTTTCA ATTTAACAGA ACCACGAGTG GATTTACACA CCTCAGGAGA ACACTCTGAA 
TTGGTTCAAG AAGAAAATTT GAGCCCAGGC ACCCAAACAC CTTCAAATGA TAAAACAAGC 
ATGTTGCAAG AATACTCCAA ATACCTCCAA CAGGCTTTCG AAAAATCCAC TAATGCAGGT 
TTTACTCTTG GACACGGTTT CCAATTTGTC AGCTTGTCTT CACCTCTCCA CAACCACACT 
TTGTTTCCAG AAAAACAGAT ATACACTACA TCTCCTTTGG AGTGTGGTTT TGGCCAGTCT 
GTTACCTCAG TGCTGCCATC TTCGTTGCCA AAGCCTCCTT TTGGGATGTT GTTTGGATCT 
CAACCAGGTC TTTATTTATC TGCTTTGGAT GCTACACATC AGCAGTTGAC ACCTTCCCAG 
GAGCTGGATG ATCTGATAGA TTCTCAGAAG AACTTAGAGA CATCATCAGC CTTCCAGTCC 
TCATCTCAGA AATTGACTAG CCAGAAGGAA CAACAGAAAA ATTTAGAGTC CTCAACGAGC 
TTTCAGATTC CATCTCAGGA GTTAGCTAGC CAGATTGATC CTCAGAAAGA CATAGAGCCT 
AGAACAACGT ACCAGATTGA GAACTTTGCA CAAGCATTCG GTTCTCAGTT TAAGTCGGGC 
AGCAGGGTGC CAATGACCTT TATCACTAAC TCTAATGGAG AAGTGGACCA TAGAGTAAGG 
ACTTCAGTGT CAGATTTCTC AGGGTATACA AATATGATGT CTGATGTAAG TGAGCCATGT 
AGTACAAGAG TAAAGACACC CACCAGCCAG AGTTACAGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153723.1
CDS	170..2851
Translation	MKIGSGFLSGGGGPSSSGGSGSGGSSGSASGGSGGGRRAEMEPTFPQSMVMFNHRLPPVTSFTRPAGTAAPPPQCVLSSSTSAAPAAEPPPPPAPDMTFKKEPAASAAAFPSQRTSWGFLQSLVSIKQEKPADPEEQPSHHHHHHHHYGGLFAGAEERSPGLGGGEGGSHGVIQDLSLLHQHAQQQPAQHHRDVLLSSGSRTDEHGNQEPKQDANVKKAKRPKPESQGIKAKRKPSASSKPLVGEGEGAVLSPSQKPHICDHCSAAFRSSYHLRRHVLIHTGERPFQCSQCSMGFIQKYLLQRHEKIHSREKPFGCDQCSMKFIQKYHMERHKRTHSGEKPYKCDTCQQYFSRTDRLLKHRRTCGEAIAKGAASAEPGSSNHNSMGNLAVLSQGNTSSSRRKSKSKSIAIENKEHKTGKTNESQMSNNINMQSYSVEMPTVSTSGSIIGTGIDELQKRVPKLIFKKGSRKNADKSYLNFVSPLPDVVGQKSLSGKPGGSLGIVSNNSVETISLLQSTSGKQGPISSNYDDAMQFSKKRRYLPTASSNSAFSINVGHMVSQQSVIQSAGVSVLDNEAPLSLIDSSALNAEIKSCHDKSGIPDEVLQSILDQYSGKSETQKEDPFNLTEPRVDLHTSGEHSELVQEENLSPGTQTPSNDKTSMLQEYSKYLQQAFEKSTNAGFTLGHGFQFVSLSSPLHNHTLFPEKQIYTTSPLECGFGQSVTSVLPSSLPKPPFGMLFGSQPGLYLSALDATHQQLTPSQELDDLIDSQKNLETSSAFQSSSQKLTSQKEQQKNLESSTSFQIPSQELASQIDPQKDIEPRTTYQIENFAQAFGSQFKSGSRVPMTFITNSNGEVDHRVRTSVSDFSGYTNMMSDVSEPCSTRVKTPTSQSYR

Target ORF information:

RefSeq Version	NM_001160251.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus zinc finger protein 281 (Zfp281), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160251.1

ORF Insert Sequence:

ATGAAAATCG GCAGCGGGTT CCTTAGCGGC GGCGGCGGTC CCAGCAGTAG CGGTGGTAGC 
GGCTCCGGCG GCAGCTCCGG CAGCGCCAGC GGCGGCAGCG GCGGCGGCAG GAGAGCAGAG 
ATGGAGCCCA CCTTCCCCCA GAGTATGGTT ATGTTCAACC ACCGGCTTCC CCCGGTCACC 
AGCTTCACCC GGCCGGCGGG GACGGCCGCC CCTCCCCCGC AGTGCGTGTT ATCCTCCTCT 
ACCTCCGCAG CCCCGGCCGC TGAGCCCCCC CCTCCGCCAG CCCCGGACAT GACTTTCAAG 
AAGGAGCCGG CGGCGTCAGC CGCGGCCTTC CCTTCGCAGA GGACCTCCTG GGGATTCTTG 
CAGTCCTTGG TGAGCATCAA GCAGGAGAAA CCTGCGGATC CCGAGGAGCA GCCGTCCCAC 
CACCACCATC ACCACCACCA CTATGGGGGG CTGTTCGCTG GGGCTGAAGA GCGATCACCA 
GGCCTAGGAG GAGGGGAAGG GGGGAGCCAC GGCGTCATCC AAGACCTCAG TCTTCTGCAC 
CAGCATGCCC AGCAGCAACC GGCCCAGCAC CACCGCGATG TATTACTGAG CAGCGGTAGC 
AGGACTGATG AGCACGGCAA CCAGGAGCCA AAGCAGGACG CTAACGTCAA AAAGGCAAAG 
AGGCCAAAGC CAGAATCTCA GGGAATCAAA GCCAAGAGGA AGCCAAGTGC ATCTTCCAAA 
CCTTTGGTTG GAGAGGGAGA AGGTGCCGTC CTGTCCCCAA GTCAGAAACC TCATATCTGT 
GATCACTGTA GTGCTGCTTT CAGGAGCTCC TACCACCTGC GGAGACATGT CCTCATCCAC 
ACGGGCGAAA GGCCTTTCCA GTGCAGCCAG TGTAGTATGG GTTTCATCCA GAAATACCTA 
CTGCAGAGAC ACGAGAAAAT TCACAGCAGA GAGAAACCCT TTGGGTGCGA TCAGTGCAGC 
ATGAAGTTTA TTCAGAAGTA CCATATGGAG AGACACAAGA GGACACATAG TGGAGAAAAG 
CCATACAAGT GTGACACTTG CCAACAGTAT TTTTCAAGGA CTGACAGATT GTTGAAGCAC 
AGGCGCACGT GTGGTGAAGC CATAGCAAAA GGAGCCGCTA GTGCAGAACC TGGGTCATCA 
AACCATAACA GTATGGGTAA CCTGGCTGTG TTGTCTCAGG GAAATACAAG TTCCTCAAGG 
AGAAAGTCGA AGTCGAAAAG CATAGCTATT GAAAATAAGG AACACAAGAC TGGCAAAACA 
AATGAATCAC AAATGTCAAA CAATATAAAC ATGCAGAGTT ATTCAGTAGA AATGCCTACT 
GTGTCTACCA GTGGGAGCAT AATTGGCACT GGAATAGATG AACTGCAGAA AAGGGTGCCA 
AAATTGATCT TTAAGAAAGG AAGCAGAAAG AATGCAGATA AAAGCTACCT TAATTTTGTG 
TCACCGTTAC CAGACGTAGT TGGGCAGAAA TCCTTGTCTG GTAAGCCAGG TGGCTCACTT 
GGCATCGTAT CGAATAATAG TGTGGAGACC ATTAGTCTTC TCCAAAGTAC AAGTGGCAAG 
CAAGGCCCGA TAAGTAGTAA TTATGATGAT GCCATGCAGT TTTCAAAGAA GAGAAGATAC 
CTACCAACTG CCAGCAGCAA CAGTGCCTTT TCTATCAATG TAGGACACAT GGTCTCCCAG 
CAGTCAGTCA TTCAGTCTGC AGGGGTCAGT GTTTTGGACA ATGAGGCGCC ATTGTCACTT 
ATTGACTCCT CAGCTCTAAA TGCTGAAATT AAGTCTTGTC ACGACAAGTC TGGAATTCCT 
GATGAGGTTT TACAAAGTAT TTTGGATCAG TACTCTGGCA AATCAGAAAC ACAGAAGGAG 
GATCCTTTCA ATTTAACAGA ACCACGAGTG GATTTACACA CCTCAGGAGA ACACTCTGAA 
TTGGTTCAAG AAGAAAATTT GAGCCCAGGC ACCCAAACAC CTTCAAATGA TAAAACAAGC 
ATGTTGCAAG AATACTCCAA ATACCTCCAA CAGGCTTTCG AAAAATCCAC TAATGCAGGT 
TTTACTCTTG GACACGGTTT CCAATTTGTC AGCTTGTCTT CACCTCTCCA CAACCACACT 
TTGTTTCCAG AAAAACAGAT ATACACTACA TCTCCTTTGG AGTGTGGTTT TGGCCAGTCT 
GTTACCTCAG TGCTGCCATC TTCGTTGCCA AAGCCTCCTT TTGGGATGTT GTTTGGATCT 
CAACCAGGTC TTTATTTATC TGCTTTGGAT GCTACACATC AGCAGTTGAC ACCTTCCCAG 
GAGCTGGATG ATCTGATAGA TTCTCAGAAG AACTTAGAGA CATCATCAGC CTTCCAGTCC 
TCATCTCAGA AATTGACTAG CCAGAAGGAA CAACAGAAAA ATTTAGAGTC CTCAACGAGC 
TTTCAGATTC CATCTCAGGA GTTAGCTAGC CAGATTGATC CTCAGAAAGA CATAGAGCCT 
AGAACAACGT ACCAGATTGA GAACTTTGCA CAAGCATTCG GTTCTCAGTT TAAGTCGGGC 
AGCAGGGTGC CAATGACCTT TATCACTAAC TCTAATGGAG AAGTGGACCA TAGAGTAAGG 
ACTTCAGTGT CAGATTTCTC AGGGTATACA AATATGATGT CTGATGTAAG TGAGCCATGT 
AGTACAAGAG TAAAGACACC CACCAGCCAG AGTTACAGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Striking a balance: regulation of transposable elements by Zfp281 and Mll2 in mouse embryonic stem cells.
Nucleic acids research45(21)12301-12310(2017 Dec)
Dai Q,Shen Y,Wang Y,Wang X,Francisco JC,Luo Z,Lin C

Zfp281 is essential for mouse epiblast maturation through transcriptional and epigenetic control of Nodal signaling.
eLife6(2017 Nov)
Huang X,Balmer S,Yang F,Fidalgo M,Li D,Guallar D,Hadjantonakis AK,Wang J

A permissive chromatin state regulated by ZFP281-AFF3 in controlling the imprinted Meg3 polycistron.
Nucleic acids research45(3)1177-1185(2017 02)
Wang Y,Shen Y,Dai Q,Yang Q,Zhang Y,Wang X,Xie W,Luo Z,Lin C

Zfp281 Coordinates Opposing Functions of Tet1 and Tet2 in Pluripotent States.
Cell stem cell19(3)355-69(2016 Sep)
Fidalgo M,Huang X,Guallar D,Sanchez-Priego C,Valdes VJ,Saunders A,Ding J,Wu WS,Clavel C,Wang J

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Zfp281 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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