Rnf103 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rnf103
Entrez Gene ID	22644
Full Name	ring finger protein 103
Synonyms	AW146237,AW212918,Zfp103,kf-1
General protein information	Preferred Names ring finger protein 103 Names E3 ubiquitin-protein ligase RNF103 RING-type E3 ubiquitin transferase RNF103 zinc finger protein 103
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 C1\|6 32.16 cM
Summary	This gene encodes a member of the RING finger family of E3 ubiquitin-protein ligases. These proteins catalyze the transfer of the ubiquitin protein from a ubiquitin E2 enzyme to a protein substrate. Homozygous knockout mice for this gene exhibit enhanced anxiety-like behavior. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015].

mRNA and Protein(s)

mRNA	Protein	Name
XM_030255320.1	XP_030111180.1	E3 ubiquitin-protein ligase RNF103 isoform X1
NM_001308303.1	NP_001295232.1	E3 ubiquitin-protein ligase RNF103 isoform 2
NM_009543.3	NP_033569.2	E3 ubiquitin-protein ligase RNF103 isoform 1
XM_011241291.2	XP_011239593.1	E3 ubiquitin-protein ligase RNF103 isoform X1
XM_006505926.3	XP_006505989.1	E3 ubiquitin-protein ligase RNF103 isoform X2
XM_006505926.4	XP_006505989.1	E3 ubiquitin-protein ligase RNF103 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	RNF103	NP_005658.1
Bos taurus (cattle)	RNF103	NP_001178337.1
	RNF103	XP_001092425.1
Gallus gallus (chicken)	RNF103	NP_001026340.1
Xenopus tropicalis (tropical clawed frog)	rnf103	XP_002937319.1
Pan troglodytes (chimpanzee)	RNF103	XP_515598.3
Mus musculus (house mouse)	Rnf103	NP_033569.2
Rattus norvegicus (Norway rat)	Rnf103	NP_445890.2
Danio rerio (zebrafish)	rnf103	NP_001004600.1
Canis lupus familiaris (dog)	RNF103	XP_532971.2

Gene Ontology
Bibliography

Related articles in PubMed

KF-1 ubiquitin ligase: anxiety suppressor model.
Hashimoto-Gotoh T, Iwabe N, Tsujimura A, Nakagawa M, Marunaka Y
Cell biochemistry and biophysics60(1-2)69-75(2011 Jun)

KF-1 Ubiquitin Ligase: An Anxiety Suppressor.
Hashimoto-Gotoh T, Iwabe N, Tsujimura A, Takao K, Miyakawa T
Frontiers in neuroscience3(1)15-24(2009 May)

Mice lacking the kf-1 gene exhibit increased anxiety- but not despair-like behavior.
Tsujimura A, Matsuki M, Takao K, Yamanishi K, Miyakawa T, Hashimoto-Gotoh T
Frontiers in behavioral neuroscience24(2008)

Identification of a novel gene with RING-H2 finger motif induced after chronic antidepressant treatment in rat brain.
Yamada M, Yamada M, Yamazaki S, Takahashi K, Nishioka G, Kudo K, Ozawa H, Yamada S, Kiuchi Y, Kamijima K, Higuchi T, Momose K
Biochemical and biophysical research communications278(1)150-7(2000 Nov)

Cloning of human and mouse cDNAs encoding novel zinc finger proteins expressed in cerebellum and hippocampus.
Yasojima K, Tsujimura A, Mizuno T, Shigeyoshi Y, Inazawa J, Kikuno R, Kuma K, Ohkubo K, Hosokawa Y, Ibata Y, Abe T, Miyata T, Matsubara K, Nakajima K, Hashimoto-Gotoh T
Biochemical and biophysical research communications231(2)481-7(1997 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies indicate that the kf-1 null mice should be useful in elucidating the molecular mechanisms of the anxiety regulation.
Title: KF-1 ubiquitin ligase: anxiety suppressor model.

The following Rnf103 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rnf103 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu69073	NM_001308303.1 Latest version!	Mus musculus ring finger protein 103 (Rnf103), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu69073	XM_011241291.2 Latest version!	Mus musculus ring finger protein 103 (Rnf103), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu37141	XM_006505926.4 Latest version!	Mus musculus ring finger protein 103 (Rnf103), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu04371	NM_009543.3 Latest version!	Mus musculus ring finger protein 103 (Rnf103), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$398.30 $569.00
OMu69073	XM_030255320.1 Latest version!	Mus musculus ring finger protein 103 (Rnf103), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu37141	XM_006505926.3	Mus musculus ring finger protein 103 (Rnf103), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rnf103 ( NM_001308303.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001295232.1 Mus musculus ring finger protein 103 (Rnf103), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu69073
Clone ID Related Accession (Same CDS sequence)	XM_030255320.1 , NM_001308303.1 , XM_011241291.2
Accession Version	NM_001308303.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase RNF103 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK134602.1, AK168644.1 and AI849515.1. Transcript Variant: This variant (2) uses an alternate splice site in its 3' terminal exon, resulting in a distinct 5' UTR and the use of a downstream in-frame start site, compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK168644.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGTGC CACAAACAAG TACATCTAAA GGGAAAGTCA TGCTTAAAGA GTACAGTGGA 
CGCAAGATTG AAGTAGAGCA CATTTTTAAG TGGATAACTG CCCATGCAGC TTCTCGGATC 
AAAACTATAT ATAATGTGGA GCATTTGAAG GAAGAATGGA ATAAAAGTGA TCAATACTGG 
GTAAAAATAT ACCTATTTGC AAACCTTGAC CAACCGCCAG CTTTCTTCTC TGCATTAAGT 
ATAAAATTTA CTGGAAGAGT TGAGTTTATT TTTGTTAATG TGGAAAATTG GAACAACAAG 
AGTTATATGA CAGATATTGG TATTTATAAC ATGCCATCAT ACATACTTAG AACTCCTGAA 
GGAATTTATA GATACGGAAA CCACACAGGT GAATTTATAT CCCTTCAGGC CATGGATTCA 
TTTCTGCGCT CATTACAACC TGAAGTAAAT GATCTGTTTG TTTTGAGTTT GGTTCTAGTT 
AATCTTATGG CTTGGATGGA CTTATTTATT ACACAAGGAG CAACCATCAA GCGATTTGTG 
GTTCTCATAA GCACTTTAGG GACATACAAT TCCCTATTAA TTATTTCTTG GCTACCTGTG 
TTGGGCTTTC TACAGCTCCC TTACTTAGAT AGCTTTTATG ACTATAGTTT AAGATTGCTG 
CGGTATTCTA ATACAACCAC ACTGGCTTCG TGGGTAAGGG CAGACTGGAT GTTTTACACT 
TCACACCCAG CCCTGTTTCT CAGTACATAC CTTGGACATG GTTTGCTAAT TGATTACTTT 
GAGAAGAAGA GACGTCGCAG CAACAATGAT GAAGTTAATG CGAATAATTT AGAATGGTTA 
TCAAGTCTGT GGGACTGGTA CACCAGCTAC CTCTTCCACC CGATTGCTTC TTTTCAGAAC 
TTTCCTGTAG ACTCTGATTG GGACGAAGAC CCTGACTTAT TCTTGGAACG GTTAGCTTTC 
CCTGACCTTT GGCTTCACCC TCTGATACCA ACTGATTATA TTAAAAACTT ACCAATGTGG 
CGATTTAAAT GTCTTGGGGT TCAGTCTGAA GAAGAAATGT CAGAGAGTTC TCAAGACACT 
GAAAATGACT CGGATAGTGA CAACATGGAC ACTTTTAGTA GTAGTAAGGA TATATTTGAA 
GATAAACAAA GCGTTGTTCA CAGTTCTCCA GGAAGAACAA GTCACTGTGA TACTGAGGCT 
TGTTCATGTG CCAATAAATG TGAGAGCAGC CCATGTGAAA GGAAGAGGAG GTCGTATGGA 
TCACATAATA CTGATGAAGA TATGGAACCG GACTGGTTAA CTTGGCCTGC GGGTACGCTG 
CACTGTACTG AATGTGTTGT TTGCCTTGAG AATTTTGAAA ATGGATGTTT GCTAATGGGG 
TTGCCTTGTG GTCACGTGTT TCACCAGAAT TGCATTGTTA TGTGGTTGGC TGGGGGCCGA 
CACTGTTGCC CTGTTTGTCG TTGGCCTTCA TATAAGAAAA AGCAGCCATA TGCACAACAA 
CAGCCGTTGT CAAATGATGT TCCATCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001295232.1
CDS	1492..3021
Translation	MSVPQTSTSKGKVMLKEYSGRKIEVEHIFKWITAHAASRIKTIYNVEHLKEEWNKSDQYWVKIYLFANLDQPPAFFSALSIKFTGRVEFIFVNVENWNNKSYMTDIGIYNMPSYILRTPEGIYRYGNHTGEFISLQAMDSFLRSLQPEVNDLFVLSLVLVNLMAWMDLFITQGATIKRFVVLISTLGTYNSLLIISWLPVLGFLQLPYLDSFYDYSLRLLRYSNTTTLASWVRADWMFYTSHPALFLSTYLGHGLLIDYFEKKRRRSNNDEVNANNLEWLSSLWDWYTSYLFHPIASFQNFPVDSDWDEDPDLFLERLAFPDLWLHPLIPTDYIKNLPMWRFKCLGVQSEEEMSESSQDTENDSDSDNMDTFSSSKDIFEDKQSVVHSSPGRTSHCDTEACSCANKCESSPCERKRRSYGSHNTDEDMEPDWLTWPAGTLHCTECVVCLENFENGCLLMGLPCGHVFHQNCIVMWLAGGRHCCPVCRWPSYKKKQPYAQQQPLSNDVPS

Target ORF information:

RefSeq Version	NM_001308303.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger protein 103 (Rnf103), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308303.1

ORF Insert Sequence:

ATGTCTGTGC CACAAACAAG TACATCTAAA GGGAAAGTCA TGCTTAAAGA GTACAGTGGA 
CGCAAGATTG AAGTAGAGCA CATTTTTAAG TGGATAACTG CCCATGCAGC TTCTCGGATC 
AAAACTATAT ATAATGTGGA GCATTTGAAG GAAGAATGGA ATAAAAGTGA TCAATACTGG 
GTAAAAATAT ACCTATTTGC AAACCTTGAC CAACCGCCAG CTTTCTTCTC TGCATTAAGT 
ATAAAATTTA CTGGAAGAGT TGAGTTTATT TTTGTTAATG TGGAAAATTG GAACAACAAG 
AGTTATATGA CAGATATTGG TATTTATAAC ATGCCATCAT ACATACTTAG AACTCCTGAA 
GGAATTTATA GATACGGAAA CCACACAGGT GAATTTATAT CCCTTCAGGC CATGGATTCA 
TTTCTGCGCT CATTACAACC TGAAGTAAAT GATCTGTTTG TTTTGAGTTT GGTTCTAGTT 
AATCTTATGG CTTGGATGGA CTTATTTATT ACACAAGGAG CAACCATCAA GCGATTTGTG 
GTTCTCATAA GCACTTTAGG GACATACAAT TCCCTATTAA TTATTTCTTG GCTACCTGTG 
TTGGGCTTTC TACAGCTCCC TTACTTAGAT AGCTTTTATG ACTATAGTTT AAGATTGCTG 
CGGTATTCTA ATACAACCAC ACTGGCTTCG TGGGTAAGGG CAGACTGGAT GTTTTACACT 
TCACACCCAG CCCTGTTTCT CAGTACATAC CTTGGACATG GTTTGCTAAT TGATTACTTT 
GAGAAGAAGA GACGTCGCAG CAACAATGAT GAAGTTAATG CGAATAATTT AGAATGGTTA 
TCAAGTCTGT GGGACTGGTA CACCAGCTAC CTCTTCCACC CGATTGCTTC TTTTCAGAAC 
TTTCCTGTAG ACTCTGATTG GGACGAAGAC CCTGACTTAT TCTTGGAACG GTTAGCTTTC 
CCTGACCTTT GGCTTCACCC TCTGATACCA ACTGATTATA TTAAAAACTT ACCAATGTGG 
CGATTTAAAT GTCTTGGGGT TCAGTCTGAA GAAGAAATGT CAGAGAGTTC TCAAGACACT 
GAAAATGACT CGGATAGTGA CAACATGGAC ACTTTTAGTA GTAGTAAGGA TATATTTGAA 
GATAAACAAA GCGTTGTTCA CAGTTCTCCA GGAAGAACAA GTCACTGTGA TACTGAGGCT 
TGTTCATGTG CCAATAAATG TGAGAGCAGC CCATGTGAAA GGAAGAGGAG GTCGTATGGA 
TCACATAATA CTGATGAAGA TATGGAACCG GACTGGTTAA CTTGGCCTGC GGGTACGCTG 
CACTGTACTG AATGTGTTGT TTGCCTTGAG AATTTTGAAA ATGGATGTTT GCTAATGGGG 
TTGCCTTGTG GTCACGTGTT TCACCAGAAT TGCATTGTTA TGTGGTTGGC TGGGGGCCGA 
CACTGTTGCC CTGTTTGTCG TTGGCCTTCA TATAAGAAAA AGCAGCCATA TGCACAACAA 
CAGCCGTTGT CAAATGATGT TCCATCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnf103 ( XM_006505926.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006505989.1 Mus musculus ring finger protein 103 (Rnf103), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu37141
Clone ID Related Accession (Same CDS sequence)	XM_006505926.3 , XM_006505926.4
Accession Version	XM_006505926.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase RNF103 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006505926.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTGA AGCTGTTTTT CTTGCTCCTG TATTTCCTGG TCCTGTTCGT CCTGGCCAGG 
TTTTTTGAGG CCATTGTGTG GTACGAGACT GGCATCTTTG CTACTCAGCT GGTGGATCCG 
GTGGCATTGA GCTTCAAGAA GCTGAAGACC ATTCTGGAGT GTCGAGGGCT GGGCTACTCC 
GGGCTGCCGG AGAAGAAAGA TGTTCGGGAG CTGGTGGAGA AGTCAGGTGA CTTGATGGAA 
GGTGAACTCT ATTCTGCTCT TAAGGAAGAA GAGGCATCTG AGTCTGTTTC TAGTACCAAT 
TTCAGTGGTG AAATGCATTT CTATGAGCTT GTGGAAGACA CAAAAGATGG CATCTGGCTG 
GTTCAGGTCA TAGCAAATGA CAGAAGTCCT TTGGTGGGTA AAATCCACTG GGAGAAAATG 
GTGAAAAAAG TGTCAAGATT TGGAATACGG ACAGGCACTT TCAACTGTTC CAGTGATCCC 
AGAGGTGACA AATGTGATAT CAAGAACTTG CTGGGGACCA AAAGGAGTAG AGTCACTGAC 
CTTGTCCATA AATGTACACA GGTACTGCAG AAGGAGAGGC TGGGTGCGTT CCACACTCAT 
CATGTCTGTG CCACAAACAA GTACATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006505989.1
CDS	787..1416
Translation	MWLKLFFLLLYFLVLFVLARFFEAIVWYETGIFATQLVDPVALSFKKLKTILECRGLGYSGLPEKKDVRELVEKSGDLMEGELYSALKEEEASESVSSTNFSGEMHFYELVEDTKDGIWLVQVIANDRSPLVGKIHWEKMVKKVSRFGIRTGTFNCSSDPRGDKCDIKNLLGTKRSRVTDLVHKCTQVLQKERLGAFHTHHVCATNKYI

Target ORF information:

RefSeq Version	XM_006505926.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger protein 103 (Rnf103), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006505926.3

ORF Insert Sequence:

ATGTGGCTGA AGCTGTTTTT CTTGCTCCTG TATTTCCTGG TCCTGTTCGT CCTGGCCAGG 
TTTTTTGAGG CCATTGTGTG GTACGAGACT GGCATCTTTG CTACTCAGCT GGTGGATCCG 
GTGGCATTGA GCTTCAAGAA GCTGAAGACC ATTCTGGAGT GTCGAGGGCT GGGCTACTCC 
GGGCTGCCGG AGAAGAAAGA TGTTCGGGAG CTGGTGGAGA AGTCAGGTGA CTTGATGGAA 
GGTGAACTCT ATTCTGCTCT TAAGGAAGAA GAGGCATCTG AGTCTGTTTC TAGTACCAAT 
TTCAGTGGTG AAATGCATTT CTATGAGCTT GTGGAAGACA CAAAAGATGG CATCTGGCTG 
GTTCAGGTCA TAGCAAATGA CAGAAGTCCT TTGGTGGGTA AAATCCACTG GGAGAAAATG 
GTGAAAAAAG TGTCAAGATT TGGAATACGG ACAGGCACTT TCAACTGTTC CAGTGATCCC 
AGAGGTGACA AATGTGATAT CAAGAACTTG CTGGGGACCA AAAGGAGTAG AGTCACTGAC 
CTTGTCCATA AATGTACACA GGTACTGCAG AAGGAGAGGC TGGGTGCGTT CCACACTCAT 
CATGTCTGTG CCACAAACAA GTACATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnf103 ( XM_011241291.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011239593.1 Mus musculus ring finger protein 103 (Rnf103), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu69073
Clone ID Related Accession (Same CDS sequence)	XM_030255320.1 , NM_001308303.1 , XM_011241291.2
Accession Version	XM_011241291.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase RNF103 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011241291.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTGC CACAAACAAG TACATCTAAA GGGAAAGTCA TGCTTAAAGA GTACAGTGGA 
CGCAAGATTG AAGTAGAGCA CATTTTTAAG TGGATAACTG CCCATGCAGC TTCTCGGATC 
AAAACTATAT ATAATGTGGA GCATTTGAAG GAAGAATGGA ATAAAAGTGA TCAATACTGG 
GTAAAAATAT ACCTATTTGC AAACCTTGAC CAACCGCCAG CTTTCTTCTC TGCATTAAGT 
ATAAAATTTA CTGGAAGAGT TGAGTTTATT TTTGTTAATG TGGAAAATTG GAACAACAAG 
AGTTATATGA CAGATATTGG TATTTATAAC ATGCCATCAT ACATACTTAG AACTCCTGAA 
GGAATTTATA GATACGGAAA CCACACAGGT GAATTTATAT CCCTTCAGGC CATGGATTCA 
TTTCTGCGCT CATTACAACC TGAAGTAAAT GATCTGTTTG TTTTGAGTTT GGTTCTAGTT 
AATCTTATGG CTTGGATGGA CTTATTTATT ACACAAGGAG CAACCATCAA GCGATTTGTG 
GTTCTCATAA GCACTTTAGG GACATACAAT TCCCTATTAA TTATTTCTTG GCTACCTGTG 
TTGGGCTTTC TACAGCTCCC TTACTTAGAT AGCTTTTATG ACTATAGTTT AAGATTGCTG 
CGGTATTCTA ATACAACCAC ACTGGCTTCG TGGGTAAGGG CAGACTGGAT GTTTTACACT 
TCACACCCAG CCCTGTTTCT CAGTACATAC CTTGGACATG GTTTGCTAAT TGATTACTTT 
GAGAAGAAGA GACGTCGCAG CAACAATGAT GAAGTTAATG CGAATAATTT AGAATGGTTA 
TCAAGTCTGT GGGACTGGTA CACCAGCTAC CTCTTCCACC CGATTGCTTC TTTTCAGAAC 
TTTCCTGTAG ACTCTGATTG GGACGAAGAC CCTGACTTAT TCTTGGAACG GTTAGCTTTC 
CCTGACCTTT GGCTTCACCC TCTGATACCA ACTGATTATA TTAAAAACTT ACCAATGTGG 
CGATTTAAAT GTCTTGGGGT TCAGTCTGAA GAAGAAATGT CAGAGAGTTC TCAAGACACT 
GAAAATGACT CGGATAGTGA CAACATGGAC ACTTTTAGTA GTAGTAAGGA TATATTTGAA 
GATAAACAAA GCGTTGTTCA CAGTTCTCCA GGAAGAACAA GTCACTGTGA TACTGAGGCT 
TGTTCATGTG CCAATAAATG TGAGAGCAGC CCATGTGAAA GGAAGAGGAG GTCGTATGGA 
TCACATAATA CTGATGAAGA TATGGAACCG GACTGGTTAA CTTGGCCTGC GGGTACGCTG 
CACTGTACTG AATGTGTTGT TTGCCTTGAG AATTTTGAAA ATGGATGTTT GCTAATGGGG 
TTGCCTTGTG GTCACGTGTT TCACCAGAAT TGCATTGTTA TGTGGTTGGC TGGGGGCCGA 
CACTGTTGCC CTGTTTGTCG TTGGCCTTCA TATAAGAAAA AGCAGCCATA TGCACAACAA 
CAGCCGTTGT CAAATGATGT TCCATCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011239593.1
CDS	60..1589
Translation	MSVPQTSTSKGKVMLKEYSGRKIEVEHIFKWITAHAASRIKTIYNVEHLKEEWNKSDQYWVKIYLFANLDQPPAFFSALSIKFTGRVEFIFVNVENWNNKSYMTDIGIYNMPSYILRTPEGIYRYGNHTGEFISLQAMDSFLRSLQPEVNDLFVLSLVLVNLMAWMDLFITQGATIKRFVVLISTLGTYNSLLIISWLPVLGFLQLPYLDSFYDYSLRLLRYSNTTTLASWVRADWMFYTSHPALFLSTYLGHGLLIDYFEKKRRRSNNDEVNANNLEWLSSLWDWYTSYLFHPIASFQNFPVDSDWDEDPDLFLERLAFPDLWLHPLIPTDYIKNLPMWRFKCLGVQSEEEMSESSQDTENDSDSDNMDTFSSSKDIFEDKQSVVHSSPGRTSHCDTEACSCANKCESSPCERKRRSYGSHNTDEDMEPDWLTWPAGTLHCTECVVCLENFENGCLLMGLPCGHVFHQNCIVMWLAGGRHCCPVCRWPSYKKKQPYAQQQPLSNDVPS

Target ORF information:

RefSeq Version	XM_011241291.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger protein 103 (Rnf103), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241291.2

ORF Insert Sequence:

ATGTCTGTGC CACAAACAAG TACATCTAAA GGGAAAGTCA TGCTTAAAGA GTACAGTGGA 
CGCAAGATTG AAGTAGAGCA CATTTTTAAG TGGATAACTG CCCATGCAGC TTCTCGGATC 
AAAACTATAT ATAATGTGGA GCATTTGAAG GAAGAATGGA ATAAAAGTGA TCAATACTGG 
GTAAAAATAT ACCTATTTGC AAACCTTGAC CAACCGCCAG CTTTCTTCTC TGCATTAAGT 
ATAAAATTTA CTGGAAGAGT TGAGTTTATT TTTGTTAATG TGGAAAATTG GAACAACAAG 
AGTTATATGA CAGATATTGG TATTTATAAC ATGCCATCAT ACATACTTAG AACTCCTGAA 
GGAATTTATA GATACGGAAA CCACACAGGT GAATTTATAT CCCTTCAGGC CATGGATTCA 
TTTCTGCGCT CATTACAACC TGAAGTAAAT GATCTGTTTG TTTTGAGTTT GGTTCTAGTT 
AATCTTATGG CTTGGATGGA CTTATTTATT ACACAAGGAG CAACCATCAA GCGATTTGTG 
GTTCTCATAA GCACTTTAGG GACATACAAT TCCCTATTAA TTATTTCTTG GCTACCTGTG 
TTGGGCTTTC TACAGCTCCC TTACTTAGAT AGCTTTTATG ACTATAGTTT AAGATTGCTG 
CGGTATTCTA ATACAACCAC ACTGGCTTCG TGGGTAAGGG CAGACTGGAT GTTTTACACT 
TCACACCCAG CCCTGTTTCT CAGTACATAC CTTGGACATG GTTTGCTAAT TGATTACTTT 
GAGAAGAAGA GACGTCGCAG CAACAATGAT GAAGTTAATG CGAATAATTT AGAATGGTTA 
TCAAGTCTGT GGGACTGGTA CACCAGCTAC CTCTTCCACC CGATTGCTTC TTTTCAGAAC 
TTTCCTGTAG ACTCTGATTG GGACGAAGAC CCTGACTTAT TCTTGGAACG GTTAGCTTTC 
CCTGACCTTT GGCTTCACCC TCTGATACCA ACTGATTATA TTAAAAACTT ACCAATGTGG 
CGATTTAAAT GTCTTGGGGT TCAGTCTGAA GAAGAAATGT CAGAGAGTTC TCAAGACACT 
GAAAATGACT CGGATAGTGA CAACATGGAC ACTTTTAGTA GTAGTAAGGA TATATTTGAA 
GATAAACAAA GCGTTGTTCA CAGTTCTCCA GGAAGAACAA GTCACTGTGA TACTGAGGCT 
TGTTCATGTG CCAATAAATG TGAGAGCAGC CCATGTGAAA GGAAGAGGAG GTCGTATGGA 
TCACATAATA CTGATGAAGA TATGGAACCG GACTGGTTAA CTTGGCCTGC GGGTACGCTG 
CACTGTACTG AATGTGTTGT TTGCCTTGAG AATTTTGAAA ATGGATGTTT GCTAATGGGG 
TTGCCTTGTG GTCACGTGTT TCACCAGAAT TGCATTGTTA TGTGGTTGGC TGGGGGCCGA 
CACTGTTGCC CTGTTTGTCG TTGGCCTTCA TATAAGAAAA AGCAGCCATA TGCACAACAA 
CAGCCGTTGT CAAATGATGT TCCATCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnf103 ( XM_006505926.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006505989.1 Mus musculus ring finger protein 103 (Rnf103), transcript variant X2, mRNA.

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CloneID	OMu37141
Clone ID Related Accession (Same CDS sequence)	XM_006505926.3 , XM_006505926.4
Accession Version	XM_006505926.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase RNF103 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006505926.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTGA AGCTGTTTTT CTTGCTCCTG TATTTCCTGG TCCTGTTCGT CCTGGCCAGG 
TTTTTTGAGG CCATTGTGTG GTACGAGACT GGCATCTTTG CTACTCAGCT GGTGGATCCG 
GTGGCATTGA GCTTCAAGAA GCTGAAGACC ATTCTGGAGT GTCGAGGGCT GGGCTACTCC 
GGGCTGCCGG AGAAGAAAGA TGTTCGGGAG CTGGTGGAGA AGTCAGGTGA CTTGATGGAA 
GGTGAACTCT ATTCTGCTCT TAAGGAAGAA GAGGCATCTG AGTCTGTTTC TAGTACCAAT 
TTCAGTGGTG AAATGCATTT CTATGAGCTT GTGGAAGACA CAAAAGATGG CATCTGGCTG 
GTTCAGGTCA TAGCAAATGA CAGAAGTCCT TTGGTGGGTA AAATCCACTG GGAGAAAATG 
GTGAAAAAAG TGTCAAGATT TGGAATACGG ACAGGCACTT TCAACTGTTC CAGTGATCCC 
AGAGGTGACA AATGTGATAT CAAGAACTTG CTGGGGACCA AAAGGAGTAG AGTCACTGAC 
CTTGTCCATA AATGTACACA GGTACTGCAG AAGGAGAGGC TGGGTGCGTT CCACACTCAT 
CATGTCTGTG CCACAAACAA GTACATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006505989.1
CDS	820..1449
Translation	MWLKLFFLLLYFLVLFVLARFFEAIVWYETGIFATQLVDPVALSFKKLKTILECRGLGYSGLPEKKDVRELVEKSGDLMEGELYSALKEEEASESVSSTNFSGEMHFYELVEDTKDGIWLVQVIANDRSPLVGKIHWEKMVKKVSRFGIRTGTFNCSSDPRGDKCDIKNLLGTKRSRVTDLVHKCTQVLQKERLGAFHTHHVCATNKYI

Target ORF information:

RefSeq Version	XM_006505926.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger protein 103 (Rnf103), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006505926.4

ORF Insert Sequence:

ATGTGGCTGA AGCTGTTTTT CTTGCTCCTG TATTTCCTGG TCCTGTTCGT CCTGGCCAGG 
TTTTTTGAGG CCATTGTGTG GTACGAGACT GGCATCTTTG CTACTCAGCT GGTGGATCCG 
GTGGCATTGA GCTTCAAGAA GCTGAAGACC ATTCTGGAGT GTCGAGGGCT GGGCTACTCC 
GGGCTGCCGG AGAAGAAAGA TGTTCGGGAG CTGGTGGAGA AGTCAGGTGA CTTGATGGAA 
GGTGAACTCT ATTCTGCTCT TAAGGAAGAA GAGGCATCTG AGTCTGTTTC TAGTACCAAT 
TTCAGTGGTG AAATGCATTT CTATGAGCTT GTGGAAGACA CAAAAGATGG CATCTGGCTG 
GTTCAGGTCA TAGCAAATGA CAGAAGTCCT TTGGTGGGTA AAATCCACTG GGAGAAAATG 
GTGAAAAAAG TGTCAAGATT TGGAATACGG ACAGGCACTT TCAACTGTTC CAGTGATCCC 
AGAGGTGACA AATGTGATAT CAAGAACTTG CTGGGGACCA AAAGGAGTAG AGTCACTGAC 
CTTGTCCATA AATGTACACA GGTACTGCAG AAGGAGAGGC TGGGTGCGTT CCACACTCAT 
CATGTCTGTG CCACAAACAA GTACATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnf103 ( NM_009543.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033569.2 Mus musculus ring finger protein 103 (Rnf103), transcript variant 1, mRNA.

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CloneID	OMu04371
Clone ID Related Accession (Same CDS sequence)	NM_009543.3
Accession Version	NM_009543.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase RNF103 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK134602.1, AK146898.1, BC137624.1 and AI849515.1. On Apr 28, 2015 this sequence version replaced NM_009543.2. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: D76445.1, AK146898.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGCTGA AGCTGTTTTT CTTGCTCCTG TATTTCCTGG TCCTGTTCGT CCTGGCCAGG 
TTTTTTGAGG CCATTGTGTG GTACGAGACT GGCATCTTTG CTACTCAGCT GGTGGATCCG 
GTGGCATTGA GCTTCAAGAA GCTGAAGACC ATTCTGGAGT GTCGAGGGCT GGGCTACTCC 
GGGCTGCCGG AGAAGAAAGA TGTTCGGGAG CTGGTGGAGA AGTCAGGTGA CTTGATGGAA 
GGTGAACTCT ATTCTGCTCT TAAGGAAGAA GAGGCATCTG AGTCTGTTTC TAGTACCAAT 
TTCAGTGGTG AAATGCATTT CTATGAGCTT GTGGAAGACA CAAAAGATGG CATCTGGCTG 
GTTCAGGTCA TAGCAAATGA CAGAAGTCCT TTGGTGGGTA AAATCCACTG GGAGAAAATG 
GTGAAAAAAG TGTCAAGATT TGGAATACGG ACAGGCACTT TCAACTGTTC CAGTGATCCC 
AGGTACTGCA GAAGGAGAGG CTGGGTGCGT TCCACACTCA TCATGTCTGT GCCACAAACA 
AGTACATCTA AAGGGAAAGT CATGCTTAAA GAGTACAGTG GACGCAAGAT TGAAGTAGAG 
CACATTTTTA AGTGGATAAC TGCCCATGCA GCTTCTCGGA TCAAAACTAT ATATAATGTG 
GAGCATTTGA AGGAAGAATG GAATAAAAGT GATCAATACT GGGTAAAAAT ATACCTATTT 
GCAAACCTTG ACCAACCGCC AGCTTTCTTC TCTGCATTAA GTATAAAATT TACTGGAAGA 
GTTGAGTTTA TTTTTGTTAA TGTGGAAAAT TGGAACAACA AGAGTTATAT GACAGATATT 
GGTATTTATA ACATGCCATC ATACATACTT AGAACTCCTG AAGGAATTTA TAGATACGGA 
AACCACACAG GTGAATTTAT ATCCCTTCAG GCCATGGATT CATTTCTGCG CTCATTACAA 
CCTGAAGTAA ATGATCTGTT TGTTTTGAGT TTGGTTCTAG TTAATCTTAT GGCTTGGATG 
GACTTATTTA TTACACAAGG AGCAACCATC AAGCGATTTG TGGTTCTCAT AAGCACTTTA 
GGGACATACA ATTCCCTATT AATTATTTCT TGGCTACCTG TGTTGGGCTT TCTACAGCTC 
CCTTACTTAG ATAGCTTTTA TGACTATAGT TTAAGATTGC TGCGGTATTC TAATACAACC 
ACACTGGCTT CGTGGGTAAG GGCAGACTGG ATGTTTTACA CTTCACACCC AGCCCTGTTT 
CTCAGTACAT ACCTTGGACA TGGTTTGCTA ATTGATTACT TTGAGAAGAA GAGACGTCGC 
AGCAACAATG ATGAAGTTAA TGCGAATAAT TTAGAATGGT TATCAAGTCT GTGGGACTGG 
TACACCAGCT ACCTCTTCCA CCCGATTGCT TCTTTTCAGA ACTTTCCTGT AGACTCTGAT 
TGGGACGAAG ACCCTGACTT ATTCTTGGAA CGGTTAGCTT TCCCTGACCT TTGGCTTCAC 
CCTCTGATAC CAACTGATTA TATTAAAAAC TTACCAATGT GGCGATTTAA ATGTCTTGGG 
GTTCAGTCTG AAGAAGAAAT GTCAGAGAGT TCTCAAGACA CTGAAAATGA CTCGGATAGT 
GACAACATGG ACACTTTTAG TAGTAGTAAG GATATATTTG AAGATAAACA AAGCGTTGTT 
CACAGTTCTC CAGGAAGAAC AAGTCACTGT GATACTGAGG CTTGTTCATG TGCCAATAAA 
TGTGAGAGCA GCCCATGTGA AAGGAAGAGG AGGTCGTATG GATCACATAA TACTGATGAA 
GATATGGAAC CGGACTGGTT AACTTGGCCT GCGGGTACGC TGCACTGTAC TGAATGTGTT 
GTTTGCCTTG AGAATTTTGA AAATGGATGT TTGCTAATGG GGTTGCCTTG TGGTCACGTG 
TTTCACCAGA ATTGCATTGT TATGTGGTTG GCTGGGGGCC GACACTGTTG CCCTGTTTGT 
CGTTGGCCTT CATATAAGAA AAAGCAGCCA TATGCACAAC AACAGCCGTT GTCAAATGAT 
GTTCCATCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033569.2
CDS	787..2838
Translation	MWLKLFFLLLYFLVLFVLARFFEAIVWYETGIFATQLVDPVALSFKKLKTILECRGLGYSGLPEKKDVRELVEKSGDLMEGELYSALKEEEASESVSSTNFSGEMHFYELVEDTKDGIWLVQVIANDRSPLVGKIHWEKMVKKVSRFGIRTGTFNCSSDPRYCRRRGWVRSTLIMSVPQTSTSKGKVMLKEYSGRKIEVEHIFKWITAHAASRIKTIYNVEHLKEEWNKSDQYWVKIYLFANLDQPPAFFSALSIKFTGRVEFIFVNVENWNNKSYMTDIGIYNMPSYILRTPEGIYRYGNHTGEFISLQAMDSFLRSLQPEVNDLFVLSLVLVNLMAWMDLFITQGATIKRFVVLISTLGTYNSLLIISWLPVLGFLQLPYLDSFYDYSLRLLRYSNTTTLASWVRADWMFYTSHPALFLSTYLGHGLLIDYFEKKRRRSNNDEVNANNLEWLSSLWDWYTSYLFHPIASFQNFPVDSDWDEDPDLFLERLAFPDLWLHPLIPTDYIKNLPMWRFKCLGVQSEEEMSESSQDTENDSDSDNMDTFSSSKDIFEDKQSVVHSSPGRTSHCDTEACSCANKCESSPCERKRRSYGSHNTDEDMEPDWLTWPAGTLHCTECVVCLENFENGCLLMGLPCGHVFHQNCIVMWLAGGRHCCPVCRWPSYKKKQPYAQQQPLSNDVPS

Target ORF information:

RefSeq Version	NM_009543.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger protein 103 (Rnf103), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009543.3

ORF Insert Sequence:

ATGTGGCTGA AGCTGTTTTT CTTGCTCCTG TATTTCCTGG TCCTGTTCGT CCTGGCCAGG 
TTTTTTGAGG CCATTGTGTG GTACGAGACT GGCATCTTTG CTACTCAGCT GGTGGATCCG 
GTGGCATTGA GCTTCAAGAA GCTGAAGACC ATTCTGGAGT GTCGAGGGCT GGGCTACTCC 
GGGCTGCCGG AGAAGAAAGA TGTTCGGGAG CTGGTGGAGA AGTCAGGTGA CTTGATGGAA 
GGTGAACTCT ATTCTGCTCT TAAGGAAGAA GAGGCATCTG AGTCTGTTTC TAGTACCAAT 
TTCAGTGGTG AAATGCATTT CTATGAGCTT GTGGAAGACA CAAAAGATGG CATCTGGCTG 
GTTCAGGTCA TAGCAAATGA CAGAAGTCCT TTGGTGGGTA AAATCCACTG GGAGAAAATG 
GTGAAAAAAG TGTCAAGATT TGGAATACGG ACAGGCACTT TCAACTGTTC CAGTGATCCC 
AGGTACTGCA GAAGGAGAGG CTGGGTGCGT TCCACACTCA TCATGTCTGT GCCACAAACA 
AGTACATCTA AAGGGAAAGT CATGCTTAAA GAGTACAGTG GACGCAAGAT TGAAGTAGAG 
CACATTTTTA AGTGGATAAC TGCCCATGCA GCTTCTCGGA TCAAAACTAT ATATAATGTG 
GAGCATTTGA AGGAAGAATG GAATAAAAGT GATCAATACT GGGTAAAAAT ATACCTATTT 
GCAAACCTTG ACCAACCGCC AGCTTTCTTC TCTGCATTAA GTATAAAATT TACTGGAAGA 
GTTGAGTTTA TTTTTGTTAA TGTGGAAAAT TGGAACAACA AGAGTTATAT GACAGATATT 
GGTATTTATA ACATGCCATC ATACATACTT AGAACTCCTG AAGGAATTTA TAGATACGGA 
AACCACACAG GTGAATTTAT ATCCCTTCAG GCCATGGATT CATTTCTGCG CTCATTACAA 
CCTGAAGTAA ATGATCTGTT TGTTTTGAGT TTGGTTCTAG TTAATCTTAT GGCTTGGATG 
GACTTATTTA TTACACAAGG AGCAACCATC AAGCGATTTG TGGTTCTCAT AAGCACTTTA 
GGGACATACA ATTCCCTATT AATTATTTCT TGGCTACCTG TGTTGGGCTT TCTACAGCTC 
CCTTACTTAG ATAGCTTTTA TGACTATAGT TTAAGATTGC TGCGGTATTC TAATACAACC 
ACACTGGCTT CGTGGGTAAG GGCAGACTGG ATGTTTTACA CTTCACACCC AGCCCTGTTT 
CTCAGTACAT ACCTTGGACA TGGTTTGCTA ATTGATTACT TTGAGAAGAA GAGACGTCGC 
AGCAACAATG ATGAAGTTAA TGCGAATAAT TTAGAATGGT TATCAAGTCT GTGGGACTGG 
TACACCAGCT ACCTCTTCCA CCCGATTGCT TCTTTTCAGA ACTTTCCTGT AGACTCTGAT 
TGGGACGAAG ACCCTGACTT ATTCTTGGAA CGGTTAGCTT TCCCTGACCT TTGGCTTCAC 
CCTCTGATAC CAACTGATTA TATTAAAAAC TTACCAATGT GGCGATTTAA ATGTCTTGGG 
GTTCAGTCTG AAGAAGAAAT GTCAGAGAGT TCTCAAGACA CTGAAAATGA CTCGGATAGT 
GACAACATGG ACACTTTTAG TAGTAGTAAG GATATATTTG AAGATAAACA AAGCGTTGTT 
CACAGTTCTC CAGGAAGAAC AAGTCACTGT GATACTGAGG CTTGTTCATG TGCCAATAAA 
TGTGAGAGCA GCCCATGTGA AAGGAAGAGG AGGTCGTATG GATCACATAA TACTGATGAA 
GATATGGAAC CGGACTGGTT AACTTGGCCT GCGGGTACGC TGCACTGTAC TGAATGTGTT 
GTTTGCCTTG AGAATTTTGA AAATGGATGT TTGCTAATGG GGTTGCCTTG TGGTCACGTG 
TTTCACCAGA ATTGCATTGT TATGTGGTTG GCTGGGGGCC GACACTGTTG CCCTGTTTGT 
CGTTGGCCTT CATATAAGAA AAAGCAGCCA TATGCACAAC AACAGCCGTT GTCAAATGAT 
GTTCCATCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnf103 ( XM_030255320.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030111180.1 Mus musculus ring finger protein 103 (Rnf103), transcript variant X1, mRNA.

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CloneID	OMu69073
Clone ID Related Accession (Same CDS sequence)	XM_030255320.1 , NM_001308303.1 , XM_011241291.2
Accession Version	XM_030255320.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase RNF103 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTGC CACAAACAAG TACATCTAAA GGGAAAGTCA TGCTTAAAGA GTACAGTGGA 
CGCAAGATTG AAGTAGAGCA CATTTTTAAG TGGATAACTG CCCATGCAGC TTCTCGGATC 
AAAACTATAT ATAATGTGGA GCATTTGAAG GAAGAATGGA ATAAAAGTGA TCAATACTGG 
GTAAAAATAT ACCTATTTGC AAACCTTGAC CAACCGCCAG CTTTCTTCTC TGCATTAAGT 
ATAAAATTTA CTGGAAGAGT TGAGTTTATT TTTGTTAATG TGGAAAATTG GAACAACAAG 
AGTTATATGA CAGATATTGG TATTTATAAC ATGCCATCAT ACATACTTAG AACTCCTGAA 
GGAATTTATA GATACGGAAA CCACACAGGT GAATTTATAT CCCTTCAGGC CATGGATTCA 
TTTCTGCGCT CATTACAACC TGAAGTAAAT GATCTGTTTG TTTTGAGTTT GGTTCTAGTT 
AATCTTATGG CTTGGATGGA CTTATTTATT ACACAAGGAG CAACCATCAA GCGATTTGTG 
GTTCTCATAA GCACTTTAGG GACATACAAT TCCCTATTAA TTATTTCTTG GCTACCTGTG 
TTGGGCTTTC TACAGCTCCC TTACTTAGAT AGCTTTTATG ACTATAGTTT AAGATTGCTG 
CGGTATTCTA ATACAACCAC ACTGGCTTCG TGGGTAAGGG CAGACTGGAT GTTTTACACT 
TCACACCCAG CCCTGTTTCT CAGTACATAC CTTGGACATG GTTTGCTAAT TGATTACTTT 
GAGAAGAAGA GACGTCGCAG CAACAATGAT GAAGTTAATG CGAATAATTT AGAATGGTTA 
TCAAGTCTGT GGGACTGGTA CACCAGCTAC CTCTTCCACC CGATTGCTTC TTTTCAGAAC 
TTTCCTGTAG ACTCTGATTG GGACGAAGAC CCTGACTTAT TCTTGGAACG GTTAGCTTTC 
CCTGACCTTT GGCTTCACCC TCTGATACCA ACTGATTATA TTAAAAACTT ACCAATGTGG 
CGATTTAAAT GTCTTGGGGT TCAGTCTGAA GAAGAAATGT CAGAGAGTTC TCAAGACACT 
GAAAATGACT CGGATAGTGA CAACATGGAC ACTTTTAGTA GTAGTAAGGA TATATTTGAA 
GATAAACAAA GCGTTGTTCA CAGTTCTCCA GGAAGAACAA GTCACTGTGA TACTGAGGCT 
TGTTCATGTG CCAATAAATG TGAGAGCAGC CCATGTGAAA GGAAGAGGAG GTCGTATGGA 
TCACATAATA CTGATGAAGA TATGGAACCG GACTGGTTAA CTTGGCCTGC GGGTACGCTG 
CACTGTACTG AATGTGTTGT TTGCCTTGAG AATTTTGAAA ATGGATGTTT GCTAATGGGG 
TTGCCTTGTG GTCACGTGTT TCACCAGAAT TGCATTGTTA TGTGGTTGGC TGGGGGCCGA 
CACTGTTGCC CTGTTTGTCG TTGGCCTTCA TATAAGAAAA AGCAGCCATA TGCACAACAA 
CAGCCGTTGT CAAATGATGT TCCATCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030111180.1
CDS	376..1905
Translation	MSVPQTSTSKGKVMLKEYSGRKIEVEHIFKWITAHAASRIKTIYNVEHLKEEWNKSDQYWVKIYLFANLDQPPAFFSALSIKFTGRVEFIFVNVENWNNKSYMTDIGIYNMPSYILRTPEGIYRYGNHTGEFISLQAMDSFLRSLQPEVNDLFVLSLVLVNLMAWMDLFITQGATIKRFVVLISTLGTYNSLLIISWLPVLGFLQLPYLDSFYDYSLRLLRYSNTTTLASWVRADWMFYTSHPALFLSTYLGHGLLIDYFEKKRRRSNNDEVNANNLEWLSSLWDWYTSYLFHPIASFQNFPVDSDWDEDPDLFLERLAFPDLWLHPLIPTDYIKNLPMWRFKCLGVQSEEEMSESSQDTENDSDSDNMDTFSSSKDIFEDKQSVVHSSPGRTSHCDTEACSCANKCESSPCERKRRSYGSHNTDEDMEPDWLTWPAGTLHCTECVVCLENFENGCLLMGLPCGHVFHQNCIVMWLAGGRHCCPVCRWPSYKKKQPYAQQQPLSNDVPS

Target ORF information:

RefSeq Version	XM_030255320.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ring finger protein 103 (Rnf103), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030255320.1

ORF Insert Sequence:

ATGTCTGTGC CACAAACAAG TACATCTAAA GGGAAAGTCA TGCTTAAAGA GTACAGTGGA 
CGCAAGATTG AAGTAGAGCA CATTTTTAAG TGGATAACTG CCCATGCAGC TTCTCGGATC 
AAAACTATAT ATAATGTGGA GCATTTGAAG GAAGAATGGA ATAAAAGTGA TCAATACTGG 
GTAAAAATAT ACCTATTTGC AAACCTTGAC CAACCGCCAG CTTTCTTCTC TGCATTAAGT 
ATAAAATTTA CTGGAAGAGT TGAGTTTATT TTTGTTAATG TGGAAAATTG GAACAACAAG 
AGTTATATGA CAGATATTGG TATTTATAAC ATGCCATCAT ACATACTTAG AACTCCTGAA 
GGAATTTATA GATACGGAAA CCACACAGGT GAATTTATAT CCCTTCAGGC CATGGATTCA 
TTTCTGCGCT CATTACAACC TGAAGTAAAT GATCTGTTTG TTTTGAGTTT GGTTCTAGTT 
AATCTTATGG CTTGGATGGA CTTATTTATT ACACAAGGAG CAACCATCAA GCGATTTGTG 
GTTCTCATAA GCACTTTAGG GACATACAAT TCCCTATTAA TTATTTCTTG GCTACCTGTG 
TTGGGCTTTC TACAGCTCCC TTACTTAGAT AGCTTTTATG ACTATAGTTT AAGATTGCTG 
CGGTATTCTA ATACAACCAC ACTGGCTTCG TGGGTAAGGG CAGACTGGAT GTTTTACACT 
TCACACCCAG CCCTGTTTCT CAGTACATAC CTTGGACATG GTTTGCTAAT TGATTACTTT 
GAGAAGAAGA GACGTCGCAG CAACAATGAT GAAGTTAATG CGAATAATTT AGAATGGTTA 
TCAAGTCTGT GGGACTGGTA CACCAGCTAC CTCTTCCACC CGATTGCTTC TTTTCAGAAC 
TTTCCTGTAG ACTCTGATTG GGACGAAGAC CCTGACTTAT TCTTGGAACG GTTAGCTTTC 
CCTGACCTTT GGCTTCACCC TCTGATACCA ACTGATTATA TTAAAAACTT ACCAATGTGG 
CGATTTAAAT GTCTTGGGGT TCAGTCTGAA GAAGAAATGT CAGAGAGTTC TCAAGACACT 
GAAAATGACT CGGATAGTGA CAACATGGAC ACTTTTAGTA GTAGTAAGGA TATATTTGAA 
GATAAACAAA GCGTTGTTCA CAGTTCTCCA GGAAGAACAA GTCACTGTGA TACTGAGGCT 
TGTTCATGTG CCAATAAATG TGAGAGCAGC CCATGTGAAA GGAAGAGGAG GTCGTATGGA 
TCACATAATA CTGATGAAGA TATGGAACCG GACTGGTTAA CTTGGCCTGC GGGTACGCTG 
CACTGTACTG AATGTGTTGT TTGCCTTGAG AATTTTGAAA ATGGATGTTT GCTAATGGGG 
TTGCCTTGTG GTCACGTGTT TCACCAGAAT TGCATTGTTA TGTGGTTGGC TGGGGGCCGA 
CACTGTTGCC CTGTTTGTCG TTGGCCTTCA TATAAGAAAA AGCAGCCATA TGCACAACAA 
CAGCCGTTGT CAAATGATGT TCCATCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

KF-1 ubiquitin ligase: anxiety suppressor model.
Cell biochemistry and biophysics60(1-2)69-75(2011 Jun)
Hashimoto-Gotoh T,Iwabe N,Tsujimura A,Nakagawa M,Marunaka Y

KF-1 Ubiquitin Ligase: An Anxiety Suppressor.
Frontiers in neuroscience3(1)15-24(2009 May)
Hashimoto-Gotoh T,Iwabe N,Tsujimura A,Takao K,Miyakawa T

Mice lacking the kf-1 gene exhibit increased anxiety- but not despair-like behavior.
Frontiers in behavioral neuroscience24(2008)
Tsujimura A,Matsuki M,Takao K,Yamanishi K,Miyakawa T,Hashimoto-Gotoh T

Identification of a novel gene with RING-H2 finger motif induced after chronic antidepressant treatment in rat brain.
Biochemical and biophysical research communications278(1)150-7(2000 Nov)
Yamada M,Yamada M,Yamazaki S,Takahashi K,Nishioka G,Kudo K,Ozawa H,Yamada S,Kiuchi Y,Kamijima K,Higuchi T,Momose K

Cloning of human and mouse cDNAs encoding novel zinc finger proteins expressed in cerebellum and hippocampus.
Biochemical and biophysical research communications231(2)481-7(1997 Feb)
Yasojima K,Tsujimura A,Mizuno T,Shigeyoshi Y,Inazawa J,Kikuno R,Kuma K,Ohkubo K,Hosokawa Y,Ibata Y,Abe T,Miyata T,Matsubara K,Nakajima K,Hashimoto-Gotoh T

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Rnf103 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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