Ube2a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ube2a
Entrez Gene ID	22209
Full Name	ubiquitin-conjugating enzyme E2A
Synonyms	HHR6A,HR6A,Mhr6a
General protein information	Preferred Names ubiquitin-conjugating enzyme E2A Names ubiquitin-conjugating enzyme E2 A E2 ubiquitin-conjugating enzyme A RAD6 homolog A ubiquitin carrier protein A ubiquitin-conjugating enzyme E2A (RAD6 homolog) ubiquitin-conjugating enzyme HR6A ubiquitin-protein ligase A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X\|X A3.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001313696.1	NP_001300625.1	ubiquitin-conjugating enzyme E2 A isoform 2
NM_019668.4	NP_062642.1	ubiquitin-conjugating enzyme E2 A isoform 1

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os07g0166800	NP_001058973.1
Xenopus tropicalis (tropical clawed frog)	ube2a	NP_001004868.1
Canis lupus familiaris (dog)	UBE2A	XP_549217.3
Gallus gallus (chicken)	UBE2A	NP_990196.1
Caenorhabditis elegans (roundworm)	ubc-1	NP_500480.1
Homo sapiens (human)	UBE2A	NP_003327.2
Pan troglodytes (chimpanzee)	UBE2A	XP_003954121.1
	UBE2A	XP_001082047.1
Mus musculus (house mouse)	Ube2a	NP_062642.1
Rattus norvegicus (Norway rat)	Ube2a	NP_001013955.1
Oryza sativa (rice)	Os03g0791800	NP_001051522.1
Oryza sativa (rice)	Os05g0182500	NP_001054819.1
Danio rerio (zebrafish)	ube2a	NP_958430.1
Arabidopsis thaliana (thale cress)	UBC1	NP_563951.1
Arabidopsis thaliana (thale cress)	UBC2	NP_565289.1

Gene Ontology
Bibliography

Related articles in PubMed

Towards Functional Annotation of the Preimplantation Transcriptome: An RNAi Screen in Mammalian Embryos.
Cui W, Dai X, Marcho C, Han Z, Zhang K, Tremblay KD, Mager J
Scientific reports637396(2016 Nov)

An essential role for UBE2A/HR6A in learning and memory and mGLUR-dependent long-term depression.
Bruinsma CF, Savelberg SM, Kool MJ, Jolfaei MA, Van Woerden GM, Baarends WM, Elgersma Y
Human molecular genetics25(1)1-8(2016 Jan)

DNA damage-specific deubiquitination regulates Rad18 functions to suppress mutagenesis.
Zeman MK, Lin JR, Freire R, Cimprich KA
The Journal of cell biology206(2)183-97(2014 Jul)

RNF168 forms a functional complex with RAD6 during the DNA damage response.
Liu C, Wang D, Wu J, Keller J, Ma T, Yu X
Journal of cell science126(Pt 9)2042-51(2013 May)

Biochemical and genetic studies of UBR3, a ubiquitin ligase with a function in olfactory and other sensory systems.
Tasaki T, Sohr R, Xia Z, Hellweg R, H?rtnagl H, Varshavsky A, Kwon YT
The Journal of biological chemistry282(25)18510-20(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results indicate a causal role of UBE2A in learning and mGLUR-dependent long-term depression
Title: An essential role for UBE2A/HR6A in learning and memory and mGLUR-dependent long-term depression.

RNF168, in complex with RAD6A or RAD6B, is activated in the DNA-damage-induced protein ubiquitination cascade.
Title: RNF168 forms a functional complex with RAD6 during the DNA damage response.

absence of mHR6A in oocytes prevents development beyond the embryonic two-cell stage
Title: The ubiquitin-conjugating DNA repair enzyme HR6A is a maternal factor essential for early embryonic development in mice.

signaling through Notch modulates the turnover of E2A proteins
Title: Notch-induced E2A ubiquitination and degradation are controlled by MAP kinase activities.

Disruption of pre-TCR expression accelerates lymphomagenesis in E2A-deficient mice.
Title: Disruption of pre-TCR expression accelerates lymphomagenesis in E2A-deficient mice.

The following Ube2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ube2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu69002	NM_001313696.1 Latest version!	Mus musculus ubiquitin-conjugating enzyme E2A (Ube2a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu11438	NM_019668.4 Latest version!	Mus musculus ubiquitin-conjugating enzyme E2A (Ube2a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ube2a ( NM_001313696.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001300625.1 Mus musculus ubiquitin-conjugating enzyme E2A (Ube2a), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu69002
Clone ID Related Accession (Same CDS sequence)	NM_001313696.1
Accession Version	NM_001313696.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	ubiquitin-conjugating enzyme E2 A isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AA013850.1, AK164074.1, AK163966.1 and BE994531.1. Transcript Variant: This variant (2) uses an alternate in-frame splice junction in the 3' end compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK163966.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGACCC CGGCCCGGCG CCGCCTCATG AGGGACTTCA AGAGGTTACA GGAAGATCCT 
CCGGCCGGAG TGAGCGGGGC TCCGTCGGAG AACAACATAA TGGTTTGGAA TGCGGTCATT 
TTTGGGCCCG AAGGGACCCC GTTTGAGGAT GGAACATTTA AGCTTACAAT TGAATTCACT 
GAAGAGTATC CTAATAAGCC ACCTACTGTT AGATTTGTCT CTAAGATGTT CCATCCTAAC 
GATGGTAGTA TATGTCTGGA TATACTTCAG AACCGTTGGA GTCCAACCTA TGATGTGTCT 
TCCATTTTAA CGTCCATACA GTCTCTATTG GATGAACCTA ATCCCAATAG TCCAGCAAAC 
AGCCAGGCTG CCCAGCTGTA CCAGGAGAAC AAGCGGGAAT ATGAAAAGCG TGTTTCTGCA 
ATAGTAGAAC AAAGCTGGCG CGACTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300625.1
CDS	192..641
Translation	MSTPARRRLMRDFKRLQEDPPAGVSGAPSENNIMVWNAVIFGPEGTPFEDGTFKLTIEFTEEYPNKPPTVRFVSKMFHPNDGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWRDC

Target ORF information:

RefSeq Version	NM_001313696.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-conjugating enzyme E2A (Ube2a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313696.1

ORF Insert Sequence:

ATGTCGACCC CGGCCCGGCG CCGCCTCATG AGGGACTTCA AGAGGTTACA GGAAGATCCT 
CCGGCCGGAG TGAGCGGGGC TCCGTCGGAG AACAACATAA TGGTTTGGAA TGCGGTCATT 
TTTGGGCCCG AAGGGACCCC GTTTGAGGAT GGAACATTTA AGCTTACAAT TGAATTCACT 
GAAGAGTATC CTAATAAGCC ACCTACTGTT AGATTTGTCT CTAAGATGTT CCATCCTAAC 
GATGGTAGTA TATGTCTGGA TATACTTCAG AACCGTTGGA GTCCAACCTA TGATGTGTCT 
TCCATTTTAA CGTCCATACA GTCTCTATTG GATGAACCTA ATCCCAATAG TCCAGCAAAC 
AGCCAGGCTG CCCAGCTGTA CCAGGAGAAC AAGCGGGAAT ATGAAAAGCG TGTTTCTGCA 
ATAGTAGAAC AAAGCTGGCG CGACTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2a ( NM_019668.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062642.1 Mus musculus ubiquitin-conjugating enzyme E2A (Ube2a), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu11438
Clone ID Related Accession (Same CDS sequence)	NM_019668.4
Accession Version	NM_019668.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	ubiquitin-conjugating enzyme E2 A isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AA013850.1, AK164074.1 and BE994531.1. On Aug 27, 2015 this sequence version replaced NM_019668.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC026053.1, AK133723.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGACCC CGGCCCGGCG CCGCCTCATG AGGGACTTCA AGAGGTTACA GGAAGATCCT 
CCGGCCGGAG TGAGCGGGGC TCCGTCGGAG AACAACATAA TGGTTTGGAA TGCGGTCATT 
TTTGGGCCCG AAGGGACCCC GTTTGAGGAT GGAACATTTA AGCTTACAAT TGAATTCACT 
GAAGAGTATC CTAATAAGCC ACCTACTGTT AGATTTGTCT CTAAGATGTT CCATCCTAAC 
GTCTATGCAG ATGGTAGTAT ATGTCTGGAT ATACTTCAGA ACCGTTGGAG TCCAACCTAT 
GATGTGTCTT CCATTTTAAC GTCCATACAG TCTCTATTGG ATGAACCTAA TCCCAATAGT 
CCAGCAAACA GCCAGGCTGC CCAGCTGTAC CAGGAGAACA AGCGGGAATA TGAAAAGCGT 
GTTTCTGCAA TAGTAGAACA AAGCTGGCGC GACTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062642.1
CDS	192..650
Translation	MSTPARRRLMRDFKRLQEDPPAGVSGAPSENNIMVWNAVIFGPEGTPFEDGTFKLTIEFTEEYPNKPPTVRFVSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWRDC

Target ORF information:

RefSeq Version	NM_019668.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-conjugating enzyme E2A (Ube2a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019668.4

ORF Insert Sequence:

ATGTCGACCC CGGCCCGGCG CCGCCTCATG AGGGACTTCA AGAGGTTACA GGAAGATCCT 
CCGGCCGGAG TGAGCGGGGC TCCGTCGGAG AACAACATAA TGGTTTGGAA TGCGGTCATT 
TTTGGGCCCG AAGGGACCCC GTTTGAGGAT GGAACATTTA AGCTTACAAT TGAATTCACT 
GAAGAGTATC CTAATAAGCC ACCTACTGTT AGATTTGTCT CTAAGATGTT CCATCCTAAC 
GTCTATGCAG ATGGTAGTAT ATGTCTGGAT ATACTTCAGA ACCGTTGGAG TCCAACCTAT 
GATGTGTCTT CCATTTTAAC GTCCATACAG TCTCTATTGG ATGAACCTAA TCCCAATAGT 
CCAGCAAACA GCCAGGCTGC CCAGCTGTAC CAGGAGAACA AGCGGGAATA TGAAAAGCGT 
GTTTCTGCAA TAGTAGAACA AAGCTGGCGC GACTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Towards Functional Annotation of the Preimplantation Transcriptome: An RNAi Screen in Mammalian Embryos.
Scientific reports637396(2016 Nov)
Cui W,Dai X,Marcho C,Han Z,Zhang K,Tremblay KD,Mager J

An essential role for UBE2A/HR6A in learning and memory and mGLUR-dependent long-term depression.
Human molecular genetics25(1)1-8(2016 Jan)
Bruinsma CF,Savelberg SM,Kool MJ,Jolfaei MA,Van Woerden GM,Baarends WM,Elgersma Y

DNA damage-specific deubiquitination regulates Rad18 functions to suppress mutagenesis.
The Journal of cell biology206(2)183-97(2014 Jul)
Zeman MK,Lin JR,Freire R,Cimprich KA

RNF168 forms a functional complex with RAD6 during the DNA damage response.
Journal of cell science126(Pt 9)2042-51(2013 May)
Liu C,Wang D,Wu J,Keller J,Ma T,Yu X

Biochemical and genetic studies of UBR3, a ubiquitin ligase with a function in olfactory and other sensory systems.
The Journal of biological chemistry282(25)18510-20(2007 Jun)
Tasaki T,Sohr R,Xia Z,Hellweg R,H?rtnagl H,Varshavsky A,Kwon YT