Tpt1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tpt1
Entrez Gene ID	22070
Full Name	tumor protein, translationally-controlled 1
Synonyms	TCTP,Trt,p21,p23
General protein information	Preferred Names tumor protein, translationally-controlled 1 Names translationally-controlled tumor protein 21 kDa polypeptide under translational control fortilin translationally regulated transcript (21 kDa)
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14 D3\|14 40.29 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_009429.3	NP_033455.1	translationally-controlled tumor protein

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TPT1	NP_001014410.1
Arabidopsis thaliana (thale cress)	AT3G05540	NP_187205.4
Caenorhabditis elegans (roundworm)	tct-1	NP_492767.1
Canis lupus familiaris (dog)	TPT1	XP_005633960.1
Mus musculus (house mouse)	Tpt1	NP_033455.1
Eremothecium gossypii	AGOS_ABR075C	NP_983021.2
Schizosaccharomyces pombe (fission yeast)	SPAC1F12.02c	NP_594328.1
Magnaporthe oryzae (rice blast fungus)	MGG_06249	XP_003717344.1
Neurospora crassa	NCU06464	XP_957355.1
Arabidopsis thaliana (thale cress)	TCTP	NP_188286.1
Xenopus tropicalis (tropical clawed frog)	tpt1	NP_001008074.1
Homo sapiens (human)	TPT1	NP_001273201.1
	TPT1	XP_002802053.1
Rattus norvegicus (Norway rat)	Tpt1	NP_446319.1
Gallus gallus (chicken)	TPT1	NP_990729.1
Danio rerio (zebrafish)	tpt1	NP_937783.1
Drosophila melanogaster (fruit fly)	Tctp	NP_650048.1
Oryza sativa (rice)	Os11g0660500	NP_001068405.1
Pan troglodytes (chimpanzee)	TPT1	XP_001139202.1
Pan troglodytes (chimpanzee)	LOC466689	XP_003952005.1
Anopheles gambiae (African malaria mosquito)	TCTP_ANOGA	XP_312257.2
Saccharomyces cerevisiae (baker's yeast)	TMA19	NP_012867.1
Kluyveromyces lactis	KLLA0C12716g	XP_452766.1

Gene Ontology
Bibliography

Related articles in PubMed

Crystal structures of murine and human Histamine-Releasing Factor (HRF/TCTP) and a model for HRF dimerisation in mast cell activation.
Dor? KA, Kashiwakura JI, McDonnell JM, Gould HJ, Kawakami T, Sutton BJ, Davies AM
Molecular immunology93216-222(2018 Jan)

TCTP regulates spindle assembly during postovulatory aging and prevents deterioration in mouse oocyte quality.
Jeon HJ, Cui XS, Guo J, Lee JM, Kim JS, Oh JS
Biochimica et biophysica acta1864(7)1328-1334(2017 07)

Fortilin binds IRE1? and prevents ER stress from signaling apoptotic cell death.
Pinkaew D, Chattopadhyay A, King MD, Chunhacha P, Liu Z, Stevenson HL, Chen Y, Sinthujaroen P, McDougal OM, Fujise K
Nature communications8(1)18(2017 05)

TPT1 (tumor protein, translationally-controlled 1) negatively regulates autophagy through the BECN1 interactome and an MTORC1-mediated pathway.
Bae SY, Byun S, Bae SH, Min DS, Woo HA, Lee K
Autophagy13(5)820-833(2017 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TCTP-mediated spindle dynamics play a key role in maintaining oocyte quality during postovulatory aging. Overexpression of TCTP is sufficient to prevent aging-associated abnormalities in mouse oocytes.
Title: TCTP regulates spindle assembly during postovulatory aging and prevents deterioration in mouse oocyte quality.

Fortilin directly interacts with the cytoplasmic domain of IRE1alpha, inhibits both kinase and endoribonuclease (RNase) activities of this stress sensor, and protects cells against apoptotic cell death at both cellular and whole animal levels.
Title: Fortilin binds IRE1? and prevents ER stress from signaling apoptotic cell death.

Leads to the enhanced and previously un-described interaction of RACK1 and TCTP.
Title: Over-expression of insulin-response element binding protein-1 (IRE-BP1) in mouse pancreatic islets increases expression of RACK1 and TCTP: Beta cell markers of high glucose sensitivity.

results demonstrate that TCTP is a microtubule-associating protein required to regulate spindle microtubule dynamics during meiotic maturation in mouse oocytes.
Title: TCTP regulates spindle microtubule dynamics by stabilizing polar microtubules during mouse oocyte meiosis.

These data suggest the presence of a novel oxidative-stress-handling pathway where the anti-p53 molecule fortilin augments the peroxidase PRX1 by protecting it against degradation and inactivation of the enzyme.
Title: Fortilin potentiates the peroxidase activity of Peroxiredoxin-1 and protects against alcohol-induced liver damage in mice.

The following Tpt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tpt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13550	NM_009429.3 Latest version!	Mus musculus tumor protein, translationally-controlled 1 (Tpt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tpt1 ( NM_009429.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033455.1 Mus musculus tumor protein, translationally-controlled 1 (Tpt1), mRNA.

Price & Availability↑

CloneID	OMu13550
Clone ID Related Accession (Same CDS sequence)	NM_009429.3
Accession Version	NM_009429.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	translationally-controlled tumor protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ063333.1, AK166584.1 and AA269935.1. On Dec 10, 2009 this sequence version replaced NM_009429.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI251570.1, BC092381.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGATCATCT ACCGGGACCT CATCAGCCAT GACGAGCTGT TCTCCGACAT CTACAAGATC 
CGGGAGATCG CGGACGGGCT GTGCCTGGAG GTGGAGGGCA AGATGGTCAG TAGAACAGAG 
GGTGCCATCG ATGACTCGCT CATCGGTGGA AATGCTTCCG CTGAAGGTCC GGAGGGCGAA 
GGTACCGAAA GCACAGTAGT CACCGGTGTT GACATTGTCA TGAACCATCA CTTACAAGAA 
ACCAGCTTCA CAAAAGAGGC TTACAAAAAG TACATCAAAG ACTACATGAA ATCACTCAAA 
GGCAAACTTG AAGAGCAGAA ACCAGAAAGA GTAAAGCCTT TTATGACTGG AGCTGCAGAG 
CAGATTAAGC ACATCCTTGC TAATTTCAAT AACTACCAGT TTTTTATTGG TGAAAACATG 
AATCCAGATG GTATGGTTGC TCTCCTGGAC TACCGTGAAG ATGGTGTGAC TCCATTCATG 
ATTTTCTTTA AGGATGGCTT AGAGATGGAG AAATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033455.1
CDS	174..692
Translation	MIIYRDLISHDELFSDIYKIREIADGLCLEVEGKMVSRTEGAIDDSLIGGNASAEGPEGEGTESTVVTGVDIVMNHHLQETSFTKEAYKKYIKDYMKSLKGKLEEQKPERVKPFMTGAAEQIKHILANFNNYQFFIGENMNPDGMVALLDYREDGVTPFMIFFKDGLEMEKC

Target ORF information:

RefSeq Version	NM_009429.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tumor protein, translationally-controlled 1 (Tpt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009429.3

ORF Insert Sequence:

ATGATCATCT ACCGGGACCT CATCAGCCAT GACGAGCTGT TCTCCGACAT CTACAAGATC 
CGGGAGATCG CGGACGGGCT GTGCCTGGAG GTGGAGGGCA AGATGGTCAG TAGAACAGAG 
GGTGCCATCG ATGACTCGCT CATCGGTGGA AATGCTTCCG CTGAAGGTCC GGAGGGCGAA 
GGTACCGAAA GCACAGTAGT CACCGGTGTT GACATTGTCA TGAACCATCA CTTACAAGAA 
ACCAGCTTCA CAAAAGAGGC TTACAAAAAG TACATCAAAG ACTACATGAA ATCACTCAAA 
GGCAAACTTG AAGAGCAGAA ACCAGAAAGA GTAAAGCCTT TTATGACTGG AGCTGCAGAG 
CAGATTAAGC ACATCCTTGC TAATTTCAAT AACTACCAGT TTTTTATTGG TGAAAACATG 
AATCCAGATG GTATGGTTGC TCTCCTGGAC TACCGTGAAG ATGGTGTGAC TCCATTCATG 
ATTTTCTTTA AGGATGGCTT AGAGATGGAG AAATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Crystal structures of murine and human Histamine-Releasing Factor (HRF/TCTP) and a model for HRF dimerisation in mast cell activation.
Molecular immunology93216-222(2018 Jan)
Dor? KA,Kashiwakura JI,McDonnell JM,Gould HJ,Kawakami T,Sutton BJ,Davies AM

TCTP regulates spindle assembly during postovulatory aging and prevents deterioration in mouse oocyte quality.
Biochimica et biophysica acta1864(7)1328-1334(2017 07)
Jeon HJ,Cui XS,Guo J,Lee JM,Kim JS,Oh JS

Fortilin binds IRE1? and prevents ER stress from signaling apoptotic cell death.
Nature communications8(1)18(2017 05)
Pinkaew D,Chattopadhyay A,King MD,Chunhacha P,Liu Z,Stevenson HL,Chen Y,Sinthujaroen P,McDougal OM,Fujise K

TPT1 (tumor protein, translationally-controlled 1) negatively regulates autophagy through the BECN1 interactome and an MTORC1-mediated pathway.
Autophagy13(5)820-833(2017 May)
Bae SY,Byun S,Bae SH,Min DS,Woo HA,Lee K