Trap1a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trap1a
Entrez Gene ID	22037
Full Name	tumor rejection antigen P1A
Synonyms	Tra-P1A
General protein information	Preferred Names tumor rejection antigen P1A Names tumor rejection antigen P815A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X F1\|X 61.35 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_011635.2	NP_035765.1	tumor rejection antigen P815A

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Trap1a	NP_035765.1
Rattus norvegicus (Norway rat)	Trap1a	XP_006257372.1

Gene Ontology
Bibliography

Related articles in PubMed

Trap1a is an X-linked and cell-intrinsic regulator of thymocyte development.
Li CS, Tang F, Zhang P, Jiang T, Saunders TL, Zheng P, Liu Y
Cellular & molecular immunology14(8)685-692(2017 Aug)

Minimal tolerance to a tumor antigen encoded by a cancer-germline gene.
Huijbers IJ, Soudja SM, Uyttenhove C, Buferne M, Inderberg-Suso EM, Colau D, Pilotte L, Powis de Tenbossche CG, Chomez P, Brasseur F, Schmitt-Verhulst AM, Van den Eynde BJ
Journal of immunology (Baltimore, Md. : 1950)188(1)111-21(2012 Jan)

Integrated microarray and ChIP analysis identifies multiple Foxa2 dependent target genes in the notochord.
Tamplin OJ, Cox BJ, Rossant J
Developmental biology360(2)415-25(2011 Dec)

A systematic screen for genes expressed in definitive endoderm by Serial Analysis of Gene Expression (SAGE).
Hou J, Charters AM, Lee SC, Zhao Y, Wu MK, Jones SJ, Marra MA, Hoodless PA
BMC developmental biology792(2007 Aug)

Different lineages of P1A-expressing cancer cells use divergent modes of immune evasion for T-cell adoptive therapy.
Bai XF, Liu JQ, Joshi PS, Wang L, Yin L, Labanowska J, Heerema N, Zheng P, Liu Y
Cancer research66(16)8241-9(2006 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Since multiple lineages of tumor cells share the tumor antigen P1A, we adoptively transferred transgenic T cells specific for P1A into mice with established P1A-expressing tumors.
Title: Different lineages of P1A-expressing cancer cells use divergent modes of immune evasion for T-cell adoptive therapy.

The following Trap1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trap1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu76900	NM_011635.2 Latest version!	Mus musculus tumor rejection antigen P1A (Trap1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trap1a ( NM_011635.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035765.1 Mus musculus tumor rejection antigen P1A (Trap1a), mRNA.

Price & Availability↑

CloneID	OMu76900
Clone ID Related Accession (Same CDS sequence)	NM_011635.2
Accession Version	NM_011635.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	tumor rejection antigen P815A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL714027.8 and AK146737.1. On Nov 25, 2015 this sequence version replaced NM_011635.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: M36387.1, AK146737.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGATA ACAAGAAGCC AGACAAAGCC CACAGTGGCT CAGGTGGTGA CGGTGATGGG 
AATAGGTGCA ATTTATTGCA CCGGTACTCC CTGGAAGAAA TTCTGCCTTA TCTAGGGTGG 
CTGGTCTTCG CTGTTGTCAC AACAAGTTTT CTGGCGCTCC AGATGTTCAT AGACGCCCTT 
TATGAGGAGC AGTATGAAAG GGATGTGGCC TGGATAGCCA GGCAAAGCAA GCGCATGTCC 
TCTGTCGATG AGGATGAAGA CGATGAGGAT GATGAGGATG ACTACTACGA CGACGAGGAC 
GACGACGACG ATGCCTTCTA TGATGATGAG GATGATGAGG AAGAAGAATT GGAGAACCTG 
ATGGATGATG AATCAGAAGA TGAGGCCGAA GAAGAGATGA GCGTGGAAAT GGGTGCCGGA 
GCTGAGGAAA TGGGTGCTGG CGCTAACTGT GCCTGTGTTC CTGGCCATCA TTTAAGGAAG 
AATGAAGTGA AGTGTAGGAT GATTTATTTC TTCCACGACC CTAATTTCCT GGTGTCTATA 
CCAGTGAACC CTAAGGAACA AATGGAGTGT AGGTGTGAAA ATGCTGATGA AGAGGTTGCA 
ATGGAAGAGG AAGAAGAAGA AGAGGAGGAG GAGGAGGAAG AGGAAATGGG AAACCCGGAT 
GGCTTCTCAC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035765.1
CDS	272..946
Translation	MSDNKKPDKAHSGSGGDGDGNRCNLLHRYSLEEILPYLGWLVFAVVTTSFLALQMFIDALYEEQYERDVAWIARQSKRMSSVDEDEDDEDDEDDYYDDEDDDDDAFYDDEDDEEEELENLMDDESEDEAEEEMSVEMGAGAEEMGAGANCACVPGHHLRKNEVKCRMIYFFHDPNFLVSIPVNPKEQMECRCENADEEVAMEEEEEEEEEEEEEEMGNPDGFSP

Target ORF information:

RefSeq Version	NM_011635.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tumor rejection antigen P1A (Trap1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011635.2

ORF Insert Sequence:

ATGTCTGATA ACAAGAAGCC AGACAAAGCC CACAGTGGCT CAGGTGGTGA CGGTGATGGG 
AATAGGTGCA ATTTATTGCA CCGGTACTCC CTGGAAGAAA TTCTGCCTTA TCTAGGGTGG 
CTGGTCTTCG CTGTTGTCAC AACAAGTTTT CTGGCGCTCC AGATGTTCAT AGACGCCCTT 
TATGAGGAGC AGTATGAAAG GGATGTGGCC TGGATAGCCA GGCAAAGCAA GCGCATGTCC 
TCTGTCGATG AGGATGAAGA CGATGAGGAT GATGAGGATG ACTACTACGA CGACGAGGAC 
GACGACGACG ATGCCTTCTA TGATGATGAG GATGATGAGG AAGAAGAATT GGAGAACCTG 
ATGGATGATG AATCAGAAGA TGAGGCCGAA GAAGAGATGA GCGTGGAAAT GGGTGCCGGA 
GCTGAGGAAA TGGGTGCTGG CGCTAACTGT GCCTGTGTTC CTGGCCATCA TTTAAGGAAG 
AATGAAGTGA AGTGTAGGAT GATTTATTTC TTCCACGACC CTAATTTCCT GGTGTCTATA 
CCAGTGAACC CTAAGGAACA AATGGAGTGT AGGTGTGAAA ATGCTGATGA AGAGGTTGCA 
ATGGAAGAGG AAGAAGAAGA AGAGGAGGAG GAGGAGGAAG AGGAAATGGG AAACCCGGAT 
GGCTTCTCAC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Trap1a is an X-linked and cell-intrinsic regulator of thymocyte development.
Cellular & molecular immunology14(8)685-692(2017 Aug)
Li CS,Tang F,Zhang P,Jiang T,Saunders TL,Zheng P,Liu Y

Minimal tolerance to a tumor antigen encoded by a cancer-germline gene.
Journal of immunology (Baltimore, Md. : 1950)188(1)111-21(2012 Jan)
Huijbers IJ,Soudja SM,Uyttenhove C,Buferne M,Inderberg-Suso EM,Colau D,Pilotte L,Powis de Tenbossche CG,Chomez P,Brasseur F,Schmitt-Verhulst AM,Van den Eynde BJ

Integrated microarray and ChIP analysis identifies multiple Foxa2 dependent target genes in the notochord.
Developmental biology360(2)415-25(2011 Dec)
Tamplin OJ,Cox BJ,Rossant J

A systematic screen for genes expressed in definitive endoderm by Serial Analysis of Gene Expression (SAGE).
BMC developmental biology792(2007 Aug)
Hou J,Charters AM,Lee SC,Zhao Y,Wu MK,Jones SJ,Marra MA,Hoodless PA

Different lineages of P1A-expressing cancer cells use divergent modes of immune evasion for T-cell adoptive therapy.
Cancer research66(16)8241-9(2006 Aug)
Bai XF,Liu JQ,Joshi PS,Wang L,Yin L,Labanowska J,Heerema N,Zheng P,Liu Y