Chdh cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chdh
Entrez Gene ID	218865
Full Name	choline dehydrogenase
Synonyms	AU041472,D630034H06Rik
General protein information	Preferred Names choline dehydrogenase Names choline dehydrogenase, mitochondrial CDH CHD
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 B

mRNA and Protein(s)

mRNA	Protein	Name
NM_175343.5	NP_780552.1	choline dehydrogenase, mitochondrial
NM_001136240.1	NP_001129712.1	choline dehydrogenase, mitochondrial
NM_172264.2	NP_758468.2	choline dehydrogenase, mitochondrial

Pathways from BioSystems

Homologies

Homo sapiens (human)	CHDH	NP_060867.2
Pan troglodytes (chimpanzee)	CHDH	XP_001173164.1
Mus musculus (house mouse)	Chdh	NP_001129712.1
Canis lupus familiaris (dog)	CHDH	XP_005632473.1
Gallus gallus (chicken)	CHDH	XP_004944627.1
Caenorhabditis elegans (roundworm)	C34C6.4	NP_495846.1
	CHDH	XP_001082768.1
Bos taurus (cattle)	CHDH	NP_001192493.1
Rattus norvegicus (Norway rat)	Chdh	NP_942026.1
Danio rerio (zebrafish)	chdh	XP_002663301.2

Gene Ontology
Bibliography

Related articles in PubMed

Betaine is accumulated via transient choline dehydrogenase activation during mouse oocyte meiotic maturation.
McClatchie T, Meredith M, Ou?draogo MO, Slow S, Lever M, Mann MRW, Zeisel SH, Trasler JM, Baltz JM
The Journal of biological chemistry292(33)13784-13794(2017 08)

Coordinately Co-opted Multiple Transposable Elements Constitute an Enhancer for wnt5a Expression in the Mammalian Secondary Palate.
Nishihara H, Kobayashi N, Kimura-Yoshida C, Yan K, Bormuth O, Ding Q, Nakanishi A, Sasaki T, Hirakawa M, Sumiyama K, Furuta Y, Tarabykin V, Matsuo I, Okada N
PLoS genetics12(10)e1006380(2016 Oct)

Deletion of murine choline dehydrogenase results in diminished sperm motility.
Johnson AR, Craciunescu CN, Guo Z, Teng YW, Thresher RJ, Blusztajn JK, Zeisel SH
FASEB journal : official publication of the Federation of American Societies for Experimental Biology24(8)2752-61(2010 Aug)

Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y
Molecular cell23(4)607-18(2006 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data, including data from studies using transgenic/knockout mice, suggest that betaine accumulates during oocyte meiotic maturation; the mechanism appears to be transient activation of Chdh; Chdh is again inactive in the zygote (that is, after fertilization).
Title: Betaine is accumulated via transient choline dehydrogenase activation during mouse oocyte meiotic maturation.

Loss of CHDH activity resulted in decreased testicular betaine and increased choline and PCho concentrations; impaired fertility was due to diminished sperm motility in the Chdh(-/-) males
Title: Deletion of murine choline dehydrogenase results in diminished sperm motility.

The following Chdh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chdh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu21550	NM_001136240.1 Latest version!	Mus musculus choline dehydrogenase (Chdh), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu21550	NM_175343.5 Latest version!	Mus musculus choline dehydrogenase (Chdh), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu21550	NM_172264.2 Latest version!	Mus musculus choline dehydrogenase (Chdh), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chdh ( NM_175343.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_780552.1 Mus musculus choline dehydrogenase (Chdh), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu21550
Clone ID Related Accession (Same CDS sequence)	NM_175343.5 , NM_001136240.1 , NM_172264.2
Accession Version	NM_175343.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	choline dehydrogenase, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK030900.1, AK164042.1 and AK052715.1. On Oct 29, 2008 this sequence version replaced NM_175343.4. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: AK030900.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN00849388 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGGCAGG TCCTTAGAGG CTGGAGGAAA GGATGGCAGA GCCCACGTGG AGCCCTGGCA 
TGGGCTGTGC AGGGCCAGCC ATGCCCCCCT TGCAGCCGCG CTGTTGCCAG TGTGGGCAAG 
GATGAGTACA CCTTTGTGGT GGTGGGCGCA GGCTCTGCTG GCTGTGTGCT GGCTAGTCGG 
CTCACCGAGG ACCCGAATCA TCGGGTGCTG CTGCTAGAGG CAGGACCTAA GGACTTGTTG 
ATGGGGAGCA AGCGGCTTCA GTGGAAGATC CACATGCCGG CTGCCCTTGT GTCCAACCTG 
TGTGATGACA AATACAACTG GTACTATCAC ACTGAGCCTC AGCCCGGCAT GGACAGTCGC 
GTGCTGTACT GGCCACGAGG CCGGGTCTGG GGTGGCTCCT CATCCCTCAA TGCCATGGTC 
TACATCCGAG GACACGCCGA GGACTATAAC CGCTGGCACC GTGAAGGTGC TGAGGGTTGG 
GACTATGCAC ACTGCCTGCC CTACTTCCGC AAGGCGCAGA GACATGAGCT GGGTGCCAAT 
ATGTACCGCG GTGGGGATGG CCCACTGCAT GTGTCTCGGG GCAAGACCAA CCACCCGCTT 
CACCAGGCCT TCCTGCAGGC AGCACGTCAG GCTGGCTACC CCTTCACTGA AGACATGAAT 
GGCTTCCAAC AGGAGGGCTT CGGCTGGATG GACATGACTG TCCACCAAGG GAAGCGCTGG 
AGCACAGCCT GCGCCTACTT GCACCCGGTG CTGAGCCGCC CCAACCTCAG GGCCGAGGTC 
CAGACACTTG TAAGCAGAGT GCTGTTTGAG GGCACCCGTG CAGTAGGTGT GGAGTACATC 
AAGGACGGCC AGAGACACAA GGCTTACGTC AGCAGGGAGG TGATCCTGAG CGGGGGAGCC 
ATCAACTCTC CACAGCTGCT CATGCTCTCT GGCGTTGGCA ATGCAGATGA CCTCAGGAAA 
CTGGATATCC CTGTGGTGTG CCACCTGCCT GGAGTTGGGC AGAACCTGCA GGACCACCTG 
GAGGTCTACG TTCAGCAAGC GTGCACACAG CCCATCACCC TCCACTCTGC CCAGAAGCCT 
CTGCGGAAGG TCTGCATCGG CCTGGAGTGG CTCTGGAGCT ACACAGGGGA CGGAGCCACA 
GCCCATCTTG AGACCGGAGG GTTCATCCGC AGCCGACCTG GGGTCCCTCA TCCGGACATC 
CAGTTCCACT TCCTGCCATC GCAAGTGATT GACCATGGGC GGAAACCCAC CCAGCAGGAA 
GCTTACCAGG TGCACGTGGG AACCATGAGG GCCACAAGTG TGGGCTGGCT GAAACTGAGA 
AGTGCCAACC CCCGGGACCA CCCTGTGATC CATCCCAACT ACTTGTCAAC AGAAACCGAT 
GTCGAGGACT TCCGTCAGTG TGTGAGGCTG TCACGAGAAA TTTTTGCCCA GGAAGCCTTG 
GCTCCATTTC GGGGAAAAGA GCTGCAGCCC GGAAGCCACG TCCAGTCAGA CAAAGAGATA 
GATGCCTTTG TGCGGGCAAA AGCAGACAGT GCTTACCACC CCTCGTGCAC CTGTAAGATG 
GGCCGGTCCT CTGACCCCAC TGCCGTGGTT GACGCGCAAA CCAAGGTCAT CGGCGTAGAA 
AACCTCAGGG TCGTTGATGC CTCCATCATG CCCAGCGTGG TCAGCGGCAA CCTGAACGCT 
CCCACGGTAA TGATTGCAGA GAAAGCAGCC GACATTATTA AGGGACACCC TGCACTTGAG 
GACAAGAATG TTCCTGTCTA CAAGCCCCAG ACTCTGGACA CCCAGCGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_780552.1
CDS	259..2049
Translation	MWQVLRGWRKGWQSPRGALAWAVQGQPCPPCSRAVASVGKDEYTFVVVGAGSAGCVLASRLTEDPNHRVLLLEAGPKDLLMGSKRLQWKIHMPAALVSNLCDDKYNWYYHTEPQPGMDSRVLYWPRGRVWGGSSSLNAMVYIRGHAEDYNRWHREGAEGWDYAHCLPYFRKAQRHELGANMYRGGDGPLHVSRGKTNHPLHQAFLQAARQAGYPFTEDMNGFQQEGFGWMDMTVHQGKRWSTACAYLHPVLSRPNLRAEVQTLVSRVLFEGTRAVGVEYIKDGQRHKAYVSREVILSGGAINSPQLLMLSGVGNADDLRKLDIPVVCHLPGVGQNLQDHLEVYVQQACTQPITLHSAQKPLRKVCIGLEWLWSYTGDGATAHLETGGFIRSRPGVPHPDIQFHFLPSQVIDHGRKPTQQEAYQVHVGTMRATSVGWLKLRSANPRDHPVIHPNYLSTETDVEDFRQCVRLSREIFAQEALAPFRGKELQPGSHVQSDKEIDAFVRAKADSAYHPSCTCKMGRSSDPTAVVDAQTKVIGVENLRVVDASIMPSVVSGNLNAPTVMIAEKAADIIKGHPALEDKNVPVYKPQTLDTQR

Target ORF information:

RefSeq Version	NM_175343.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus choline dehydrogenase (Chdh), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175343.5

ORF Insert Sequence:

ATGTGGCAGG TCCTTAGAGG CTGGAGGAAA GGATGGCAGA GCCCACGTGG AGCCCTGGCA 
TGGGCTGTGC AGGGCCAGCC ATGCCCCCCT TGCAGCCGCG CTGTTGCCAG TGTGGGCAAG 
GATGAGTACA CCTTTGTGGT GGTGGGCGCA GGCTCTGCTG GCTGTGTGCT GGCTAGTCGG 
CTCACCGAGG ACCCGAATCA TCGGGTGCTG CTGCTAGAGG CAGGACCTAA GGACTTGTTG 
ATGGGGAGCA AGCGGCTTCA GTGGAAGATC CACATGCCGG CTGCCCTTGT GTCCAACCTG 
TGTGATGACA AATACAACTG GTACTATCAC ACTGAGCCTC AGCCCGGCAT GGACAGTCGC 
GTGCTGTACT GGCCACGAGG CCGGGTCTGG GGTGGCTCCT CATCCCTCAA TGCCATGGTC 
TACATCCGAG GACACGCCGA GGACTATAAC CGCTGGCACC GTGAAGGTGC TGAGGGTTGG 
GACTATGCAC ACTGCCTGCC CTACTTCCGC AAGGCGCAGA GACATGAGCT GGGTGCCAAT 
ATGTACCGCG GTGGGGATGG CCCACTGCAT GTGTCTCGGG GCAAGACCAA CCACCCGCTT 
CACCAGGCCT TCCTGCAGGC AGCACGTCAG GCTGGCTACC CCTTCACTGA AGACATGAAT 
GGCTTCCAAC AGGAGGGCTT CGGCTGGATG GACATGACTG TCCACCAAGG GAAGCGCTGG 
AGCACAGCCT GCGCCTACTT GCACCCGGTG CTGAGCCGCC CCAACCTCAG GGCCGAGGTC 
CAGACACTTG TAAGCAGAGT GCTGTTTGAG GGCACCCGTG CAGTAGGTGT GGAGTACATC 
AAGGACGGCC AGAGACACAA GGCTTACGTC AGCAGGGAGG TGATCCTGAG CGGGGGAGCC 
ATCAACTCTC CACAGCTGCT CATGCTCTCT GGCGTTGGCA ATGCAGATGA CCTCAGGAAA 
CTGGATATCC CTGTGGTGTG CCACCTGCCT GGAGTTGGGC AGAACCTGCA GGACCACCTG 
GAGGTCTACG TTCAGCAAGC GTGCACACAG CCCATCACCC TCCACTCTGC CCAGAAGCCT 
CTGCGGAAGG TCTGCATCGG CCTGGAGTGG CTCTGGAGCT ACACAGGGGA CGGAGCCACA 
GCCCATCTTG AGACCGGAGG GTTCATCCGC AGCCGACCTG GGGTCCCTCA TCCGGACATC 
CAGTTCCACT TCCTGCCATC GCAAGTGATT GACCATGGGC GGAAACCCAC CCAGCAGGAA 
GCTTACCAGG TGCACGTGGG AACCATGAGG GCCACAAGTG TGGGCTGGCT GAAACTGAGA 
AGTGCCAACC CCCGGGACCA CCCTGTGATC CATCCCAACT ACTTGTCAAC AGAAACCGAT 
GTCGAGGACT TCCGTCAGTG TGTGAGGCTG TCACGAGAAA TTTTTGCCCA GGAAGCCTTG 
GCTCCATTTC GGGGAAAAGA GCTGCAGCCC GGAAGCCACG TCCAGTCAGA CAAAGAGATA 
GATGCCTTTG TGCGGGCAAA AGCAGACAGT GCTTACCACC CCTCGTGCAC CTGTAAGATG 
GGCCGGTCCT CTGACCCCAC TGCCGTGGTT GACGCGCAAA CCAAGGTCAT CGGCGTAGAA 
AACCTCAGGG TCGTTGATGC CTCCATCATG CCCAGCGTGG TCAGCGGCAA CCTGAACGCT 
CCCACGGTAA TGATTGCAGA GAAAGCAGCC GACATTATTA AGGGACACCC TGCACTTGAG 
GACAAGAATG TTCCTGTCTA CAAGCCCCAG ACTCTGGACA CCCAGCGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chdh ( NM_001136240.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001129712.1 Mus musculus choline dehydrogenase (Chdh), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu21550
Clone ID Related Accession (Same CDS sequence)	NM_175343.5 , NM_001136240.1 , NM_172264.2
Accession Version	NM_001136240.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	choline dehydrogenase, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY773937.1, AK164042.1, AK030900.1 and AK052715.1. Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: AK052715.1, AK164042.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGGCAGG TCCTTAGAGG CTGGAGGAAA GGATGGCAGA GCCCACGTGG AGCCCTGGCA 
TGGGCTGTGC AGGGCCAGCC ATGCCCCCCT TGCAGCCGCG CTGTTGCCAG TGTGGGCAAG 
GATGAGTACA CCTTTGTGGT GGTGGGCGCA GGCTCTGCTG GCTGTGTGCT GGCTAGTCGG 
CTCACCGAGG ACCCGAATCA TCGGGTGCTG CTGCTAGAGG CAGGACCTAA GGACTTGTTG 
ATGGGGAGCA AGCGGCTTCA GTGGAAGATC CACATGCCGG CTGCCCTTGT GTCCAACCTG 
TGTGATGACA AATACAACTG GTACTATCAC ACTGAGCCTC AGCCCGGCAT GGACAGTCGC 
GTGCTGTACT GGCCACGAGG CCGGGTCTGG GGTGGCTCCT CATCCCTCAA TGCCATGGTC 
TACATCCGAG GACACGCCGA GGACTATAAC CGCTGGCACC GTGAAGGTGC TGAGGGTTGG 
GACTATGCAC ACTGCCTGCC CTACTTCCGC AAGGCGCAGA GACATGAGCT GGGTGCCAAT 
ATGTACCGCG GTGGGGATGG CCCACTGCAT GTGTCTCGGG GCAAGACCAA CCACCCGCTT 
CACCAGGCCT TCCTGCAGGC AGCACGTCAG GCTGGCTACC CCTTCACTGA AGACATGAAT 
GGCTTCCAAC AGGAGGGCTT CGGCTGGATG GACATGACTG TCCACCAAGG GAAGCGCTGG 
AGCACAGCCT GCGCCTACTT GCACCCGGTG CTGAGCCGCC CCAACCTCAG GGCCGAGGTC 
CAGACACTTG TAAGCAGAGT GCTGTTTGAG GGCACCCGTG CAGTAGGTGT GGAGTACATC 
AAGGACGGCC AGAGACACAA GGCTTACGTC AGCAGGGAGG TGATCCTGAG CGGGGGAGCC 
ATCAACTCTC CACAGCTGCT CATGCTCTCT GGCGTTGGCA ATGCAGATGA CCTCAGGAAA 
CTGGATATCC CTGTGGTGTG CCACCTGCCT GGAGTTGGGC AGAACCTGCA GGACCACCTG 
GAGGTCTACG TTCAGCAAGC GTGCACACAG CCCATCACCC TCCACTCTGC CCAGAAGCCT 
CTGCGGAAGG TCTGCATCGG CCTGGAGTGG CTCTGGAGCT ACACAGGGGA CGGAGCCACA 
GCCCATCTTG AGACCGGAGG GTTCATCCGC AGCCGACCTG GGGTCCCTCA TCCGGACATC 
CAGTTCCACT TCCTGCCATC GCAAGTGATT GACCATGGGC GGAAACCCAC CCAGCAGGAA 
GCTTACCAGG TGCACGTGGG AACCATGAGG GCCACAAGTG TGGGCTGGCT GAAACTGAGA 
AGTGCCAACC CCCGGGACCA CCCTGTGATC CATCCCAACT ACTTGTCAAC AGAAACCGAT 
GTCGAGGACT TCCGTCAGTG TGTGAGGCTG TCACGAGAAA TTTTTGCCCA GGAAGCCTTG 
GCTCCATTTC GGGGAAAAGA GCTGCAGCCC GGAAGCCACG TCCAGTCAGA CAAAGAGATA 
GATGCCTTTG TGCGGGCAAA AGCAGACAGT GCTTACCACC CCTCGTGCAC CTGTAAGATG 
GGCCGGTCCT CTGACCCCAC TGCCGTGGTT GACGCGCAAA CCAAGGTCAT CGGCGTAGAA 
AACCTCAGGG TCGTTGATGC CTCCATCATG CCCAGCGTGG TCAGCGGCAA CCTGAACGCT 
CCCACGGTAA TGATTGCAGA GAAAGCAGCC GACATTATTA AGGGACACCC TGCACTTGAG 
GACAAGAATG TTCCTGTCTA CAAGCCCCAG ACTCTGGACA CCCAGCGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129712.1
CDS	286..2076
Translation	MWQVLRGWRKGWQSPRGALAWAVQGQPCPPCSRAVASVGKDEYTFVVVGAGSAGCVLASRLTEDPNHRVLLLEAGPKDLLMGSKRLQWKIHMPAALVSNLCDDKYNWYYHTEPQPGMDSRVLYWPRGRVWGGSSSLNAMVYIRGHAEDYNRWHREGAEGWDYAHCLPYFRKAQRHELGANMYRGGDGPLHVSRGKTNHPLHQAFLQAARQAGYPFTEDMNGFQQEGFGWMDMTVHQGKRWSTACAYLHPVLSRPNLRAEVQTLVSRVLFEGTRAVGVEYIKDGQRHKAYVSREVILSGGAINSPQLLMLSGVGNADDLRKLDIPVVCHLPGVGQNLQDHLEVYVQQACTQPITLHSAQKPLRKVCIGLEWLWSYTGDGATAHLETGGFIRSRPGVPHPDIQFHFLPSQVIDHGRKPTQQEAYQVHVGTMRATSVGWLKLRSANPRDHPVIHPNYLSTETDVEDFRQCVRLSREIFAQEALAPFRGKELQPGSHVQSDKEIDAFVRAKADSAYHPSCTCKMGRSSDPTAVVDAQTKVIGVENLRVVDASIMPSVVSGNLNAPTVMIAEKAADIIKGHPALEDKNVPVYKPQTLDTQR

Target ORF information:

RefSeq Version	NM_001136240.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus choline dehydrogenase (Chdh), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136240.1

ORF Insert Sequence:

ATGTGGCAGG TCCTTAGAGG CTGGAGGAAA GGATGGCAGA GCCCACGTGG AGCCCTGGCA 
TGGGCTGTGC AGGGCCAGCC ATGCCCCCCT TGCAGCCGCG CTGTTGCCAG TGTGGGCAAG 
GATGAGTACA CCTTTGTGGT GGTGGGCGCA GGCTCTGCTG GCTGTGTGCT GGCTAGTCGG 
CTCACCGAGG ACCCGAATCA TCGGGTGCTG CTGCTAGAGG CAGGACCTAA GGACTTGTTG 
ATGGGGAGCA AGCGGCTTCA GTGGAAGATC CACATGCCGG CTGCCCTTGT GTCCAACCTG 
TGTGATGACA AATACAACTG GTACTATCAC ACTGAGCCTC AGCCCGGCAT GGACAGTCGC 
GTGCTGTACT GGCCACGAGG CCGGGTCTGG GGTGGCTCCT CATCCCTCAA TGCCATGGTC 
TACATCCGAG GACACGCCGA GGACTATAAC CGCTGGCACC GTGAAGGTGC TGAGGGTTGG 
GACTATGCAC ACTGCCTGCC CTACTTCCGC AAGGCGCAGA GACATGAGCT GGGTGCCAAT 
ATGTACCGCG GTGGGGATGG CCCACTGCAT GTGTCTCGGG GCAAGACCAA CCACCCGCTT 
CACCAGGCCT TCCTGCAGGC AGCACGTCAG GCTGGCTACC CCTTCACTGA AGACATGAAT 
GGCTTCCAAC AGGAGGGCTT CGGCTGGATG GACATGACTG TCCACCAAGG GAAGCGCTGG 
AGCACAGCCT GCGCCTACTT GCACCCGGTG CTGAGCCGCC CCAACCTCAG GGCCGAGGTC 
CAGACACTTG TAAGCAGAGT GCTGTTTGAG GGCACCCGTG CAGTAGGTGT GGAGTACATC 
AAGGACGGCC AGAGACACAA GGCTTACGTC AGCAGGGAGG TGATCCTGAG CGGGGGAGCC 
ATCAACTCTC CACAGCTGCT CATGCTCTCT GGCGTTGGCA ATGCAGATGA CCTCAGGAAA 
CTGGATATCC CTGTGGTGTG CCACCTGCCT GGAGTTGGGC AGAACCTGCA GGACCACCTG 
GAGGTCTACG TTCAGCAAGC GTGCACACAG CCCATCACCC TCCACTCTGC CCAGAAGCCT 
CTGCGGAAGG TCTGCATCGG CCTGGAGTGG CTCTGGAGCT ACACAGGGGA CGGAGCCACA 
GCCCATCTTG AGACCGGAGG GTTCATCCGC AGCCGACCTG GGGTCCCTCA TCCGGACATC 
CAGTTCCACT TCCTGCCATC GCAAGTGATT GACCATGGGC GGAAACCCAC CCAGCAGGAA 
GCTTACCAGG TGCACGTGGG AACCATGAGG GCCACAAGTG TGGGCTGGCT GAAACTGAGA 
AGTGCCAACC CCCGGGACCA CCCTGTGATC CATCCCAACT ACTTGTCAAC AGAAACCGAT 
GTCGAGGACT TCCGTCAGTG TGTGAGGCTG TCACGAGAAA TTTTTGCCCA GGAAGCCTTG 
GCTCCATTTC GGGGAAAAGA GCTGCAGCCC GGAAGCCACG TCCAGTCAGA CAAAGAGATA 
GATGCCTTTG TGCGGGCAAA AGCAGACAGT GCTTACCACC CCTCGTGCAC CTGTAAGATG 
GGCCGGTCCT CTGACCCCAC TGCCGTGGTT GACGCGCAAA CCAAGGTCAT CGGCGTAGAA 
AACCTCAGGG TCGTTGATGC CTCCATCATG CCCAGCGTGG TCAGCGGCAA CCTGAACGCT 
CCCACGGTAA TGATTGCAGA GAAAGCAGCC GACATTATTA AGGGACACCC TGCACTTGAG 
GACAAGAATG TTCCTGTCTA CAAGCCCCAG ACTCTGGACA CCCAGCGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chdh ( NM_172264.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_758468.2 Mus musculus choline dehydrogenase (Chdh), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu21550
Clone ID Related Accession (Same CDS sequence)	NM_175343.5 , NM_001136240.1 , NM_172264.2
Accession Version	NM_172264.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	choline dehydrogenase, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT009536.7, AK164042.1, AK030900.1 and AK052715.1. On Oct 29, 2008 this sequence version replaced NM_172264.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC039186.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGGCAGG TCCTTAGAGG CTGGAGGAAA GGATGGCAGA GCCCACGTGG AGCCCTGGCA 
TGGGCTGTGC AGGGCCAGCC ATGCCCCCCT TGCAGCCGCG CTGTTGCCAG TGTGGGCAAG 
GATGAGTACA CCTTTGTGGT GGTGGGCGCA GGCTCTGCTG GCTGTGTGCT GGCTAGTCGG 
CTCACCGAGG ACCCGAATCA TCGGGTGCTG CTGCTAGAGG CAGGACCTAA GGACTTGTTG 
ATGGGGAGCA AGCGGCTTCA GTGGAAGATC CACATGCCGG CTGCCCTTGT GTCCAACCTG 
TGTGATGACA AATACAACTG GTACTATCAC ACTGAGCCTC AGCCCGGCAT GGACAGTCGC 
GTGCTGTACT GGCCACGAGG CCGGGTCTGG GGTGGCTCCT CATCCCTCAA TGCCATGGTC 
TACATCCGAG GACACGCCGA GGACTATAAC CGCTGGCACC GTGAAGGTGC TGAGGGTTGG 
GACTATGCAC ACTGCCTGCC CTACTTCCGC AAGGCGCAGA GACATGAGCT GGGTGCCAAT 
ATGTACCGCG GTGGGGATGG CCCACTGCAT GTGTCTCGGG GCAAGACCAA CCACCCGCTT 
CACCAGGCCT TCCTGCAGGC AGCACGTCAG GCTGGCTACC CCTTCACTGA AGACATGAAT 
GGCTTCCAAC AGGAGGGCTT CGGCTGGATG GACATGACTG TCCACCAAGG GAAGCGCTGG 
AGCACAGCCT GCGCCTACTT GCACCCGGTG CTGAGCCGCC CCAACCTCAG GGCCGAGGTC 
CAGACACTTG TAAGCAGAGT GCTGTTTGAG GGCACCCGTG CAGTAGGTGT GGAGTACATC 
AAGGACGGCC AGAGACACAA GGCTTACGTC AGCAGGGAGG TGATCCTGAG CGGGGGAGCC 
ATCAACTCTC CACAGCTGCT CATGCTCTCT GGCGTTGGCA ATGCAGATGA CCTCAGGAAA 
CTGGATATCC CTGTGGTGTG CCACCTGCCT GGAGTTGGGC AGAACCTGCA GGACCACCTG 
GAGGTCTACG TTCAGCAAGC GTGCACACAG CCCATCACCC TCCACTCTGC CCAGAAGCCT 
CTGCGGAAGG TCTGCATCGG CCTGGAGTGG CTCTGGAGCT ACACAGGGGA CGGAGCCACA 
GCCCATCTTG AGACCGGAGG GTTCATCCGC AGCCGACCTG GGGTCCCTCA TCCGGACATC 
CAGTTCCACT TCCTGCCATC GCAAGTGATT GACCATGGGC GGAAACCCAC CCAGCAGGAA 
GCTTACCAGG TGCACGTGGG AACCATGAGG GCCACAAGTG TGGGCTGGCT GAAACTGAGA 
AGTGCCAACC CCCGGGACCA CCCTGTGATC CATCCCAACT ACTTGTCAAC AGAAACCGAT 
GTCGAGGACT TCCGTCAGTG TGTGAGGCTG TCACGAGAAA TTTTTGCCCA GGAAGCCTTG 
GCTCCATTTC GGGGAAAAGA GCTGCAGCCC GGAAGCCACG TCCAGTCAGA CAAAGAGATA 
GATGCCTTTG TGCGGGCAAA AGCAGACAGT GCTTACCACC CCTCGTGCAC CTGTAAGATG 
GGCCGGTCCT CTGACCCCAC TGCCGTGGTT GACGCGCAAA CCAAGGTCAT CGGCGTAGAA 
AACCTCAGGG TCGTTGATGC CTCCATCATG CCCAGCGTGG TCAGCGGCAA CCTGAACGCT 
CCCACGGTAA TGATTGCAGA GAAAGCAGCC GACATTATTA AGGGACACCC TGCACTTGAG 
GACAAGAATG TTCCTGTCTA CAAGCCCCAG ACTCTGGACA CCCAGCGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_758468.2
CDS	84..1874
Translation	MWQVLRGWRKGWQSPRGALAWAVQGQPCPPCSRAVASVGKDEYTFVVVGAGSAGCVLASRLTEDPNHRVLLLEAGPKDLLMGSKRLQWKIHMPAALVSNLCDDKYNWYYHTEPQPGMDSRVLYWPRGRVWGGSSSLNAMVYIRGHAEDYNRWHREGAEGWDYAHCLPYFRKAQRHELGANMYRGGDGPLHVSRGKTNHPLHQAFLQAARQAGYPFTEDMNGFQQEGFGWMDMTVHQGKRWSTACAYLHPVLSRPNLRAEVQTLVSRVLFEGTRAVGVEYIKDGQRHKAYVSREVILSGGAINSPQLLMLSGVGNADDLRKLDIPVVCHLPGVGQNLQDHLEVYVQQACTQPITLHSAQKPLRKVCIGLEWLWSYTGDGATAHLETGGFIRSRPGVPHPDIQFHFLPSQVIDHGRKPTQQEAYQVHVGTMRATSVGWLKLRSANPRDHPVIHPNYLSTETDVEDFRQCVRLSREIFAQEALAPFRGKELQPGSHVQSDKEIDAFVRAKADSAYHPSCTCKMGRSSDPTAVVDAQTKVIGVENLRVVDASIMPSVVSGNLNAPTVMIAEKAADIIKGHPALEDKNVPVYKPQTLDTQR

Target ORF information:

RefSeq Version	NM_172264.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus choline dehydrogenase (Chdh), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172264.2

ORF Insert Sequence:

ATGTGGCAGG TCCTTAGAGG CTGGAGGAAA GGATGGCAGA GCCCACGTGG AGCCCTGGCA 
TGGGCTGTGC AGGGCCAGCC ATGCCCCCCT TGCAGCCGCG CTGTTGCCAG TGTGGGCAAG 
GATGAGTACA CCTTTGTGGT GGTGGGCGCA GGCTCTGCTG GCTGTGTGCT GGCTAGTCGG 
CTCACCGAGG ACCCGAATCA TCGGGTGCTG CTGCTAGAGG CAGGACCTAA GGACTTGTTG 
ATGGGGAGCA AGCGGCTTCA GTGGAAGATC CACATGCCGG CTGCCCTTGT GTCCAACCTG 
TGTGATGACA AATACAACTG GTACTATCAC ACTGAGCCTC AGCCCGGCAT GGACAGTCGC 
GTGCTGTACT GGCCACGAGG CCGGGTCTGG GGTGGCTCCT CATCCCTCAA TGCCATGGTC 
TACATCCGAG GACACGCCGA GGACTATAAC CGCTGGCACC GTGAAGGTGC TGAGGGTTGG 
GACTATGCAC ACTGCCTGCC CTACTTCCGC AAGGCGCAGA GACATGAGCT GGGTGCCAAT 
ATGTACCGCG GTGGGGATGG CCCACTGCAT GTGTCTCGGG GCAAGACCAA CCACCCGCTT 
CACCAGGCCT TCCTGCAGGC AGCACGTCAG GCTGGCTACC CCTTCACTGA AGACATGAAT 
GGCTTCCAAC AGGAGGGCTT CGGCTGGATG GACATGACTG TCCACCAAGG GAAGCGCTGG 
AGCACAGCCT GCGCCTACTT GCACCCGGTG CTGAGCCGCC CCAACCTCAG GGCCGAGGTC 
CAGACACTTG TAAGCAGAGT GCTGTTTGAG GGCACCCGTG CAGTAGGTGT GGAGTACATC 
AAGGACGGCC AGAGACACAA GGCTTACGTC AGCAGGGAGG TGATCCTGAG CGGGGGAGCC 
ATCAACTCTC CACAGCTGCT CATGCTCTCT GGCGTTGGCA ATGCAGATGA CCTCAGGAAA 
CTGGATATCC CTGTGGTGTG CCACCTGCCT GGAGTTGGGC AGAACCTGCA GGACCACCTG 
GAGGTCTACG TTCAGCAAGC GTGCACACAG CCCATCACCC TCCACTCTGC CCAGAAGCCT 
CTGCGGAAGG TCTGCATCGG CCTGGAGTGG CTCTGGAGCT ACACAGGGGA CGGAGCCACA 
GCCCATCTTG AGACCGGAGG GTTCATCCGC AGCCGACCTG GGGTCCCTCA TCCGGACATC 
CAGTTCCACT TCCTGCCATC GCAAGTGATT GACCATGGGC GGAAACCCAC CCAGCAGGAA 
GCTTACCAGG TGCACGTGGG AACCATGAGG GCCACAAGTG TGGGCTGGCT GAAACTGAGA 
AGTGCCAACC CCCGGGACCA CCCTGTGATC CATCCCAACT ACTTGTCAAC AGAAACCGAT 
GTCGAGGACT TCCGTCAGTG TGTGAGGCTG TCACGAGAAA TTTTTGCCCA GGAAGCCTTG 
GCTCCATTTC GGGGAAAAGA GCTGCAGCCC GGAAGCCACG TCCAGTCAGA CAAAGAGATA 
GATGCCTTTG TGCGGGCAAA AGCAGACAGT GCTTACCACC CCTCGTGCAC CTGTAAGATG 
GGCCGGTCCT CTGACCCCAC TGCCGTGGTT GACGCGCAAA CCAAGGTCAT CGGCGTAGAA 
AACCTCAGGG TCGTTGATGC CTCCATCATG CCCAGCGTGG TCAGCGGCAA CCTGAACGCT 
CCCACGGTAA TGATTGCAGA GAAAGCAGCC GACATTATTA AGGGACACCC TGCACTTGAG 
GACAAGAATG TTCCTGTCTA CAAGCCCCAG ACTCTGGACA CCCAGCGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Betaine is accumulated via transient choline dehydrogenase activation during mouse oocyte meiotic maturation.
The Journal of biological chemistry292(33)13784-13794(2017 08)
McClatchie T,Meredith M,Ou?draogo MO,Slow S,Lever M,Mann MRW,Zeisel SH,Trasler JM,Baltz JM

Coordinately Co-opted Multiple Transposable Elements Constitute an Enhancer for wnt5a Expression in the Mammalian Secondary Palate.
PLoS genetics12(10)e1006380(2016 Oct)
Nishihara H,Kobayashi N,Kimura-Yoshida C,Yan K,Bormuth O,Ding Q,Nakanishi A,Sasaki T,Hirakawa M,Sumiyama K,Furuta Y,Tarabykin V,Matsuo I,Okada N

Deletion of murine choline dehydrogenase results in diminished sperm motility.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology24(8)2752-61(2010 Aug)
Johnson AR,Craciunescu CN,Guo Z,Teng YW,Thresher RJ,Blusztajn JK,Zeisel SH

Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
Molecular cell23(4)607-18(2006 Aug)
Kim SC,Sprung R,Chen Y,Xu Y,Ball H,Pei J,Cheng T,Kho Y,Xiao H,Xiao L,Grishin NV,White M,Yang XJ,Zhao Y

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Chdh cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

FAX

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