Tbrg1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tbrg1
Entrez Gene ID	21376
Full Name	transforming growth factor beta regulated gene 1
Synonyms	AA408552,AA409675,Niam,Tb-5,mFLJ00213
General protein information	Preferred Names transforming growth factor beta regulated gene 1 Names transforming growth factor beta regulator 1 nuclear interactor of ARF and Mdm2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 A4

mRNA and Protein(s)

mRNA	Protein	Name
NM_025289.3	NP_079565.2	transforming growth factor beta regulator 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TBRG1	NP_116200.2
	TBRG1	XP_001109750.1
Canis lupus familiaris (dog)	TBRG1	XP_546430.2
Danio rerio (zebrafish)	tbrg1	NP_001107918.2
Drosophila melanogaster (fruit fly)	CG31111	NP_733074.1
Pan troglodytes (chimpanzee)	TBRG1	XP_522238.2
Rattus norvegicus (Norway rat)	Tbrg1	NP_001009344.2
Rattus norvegicus (Norway rat)	LOC100361475	XP_002728997.1
Rattus norvegicus (Norway rat)	LOC100911055	XP_003752012.1
Xenopus tropicalis (tropical clawed frog)	tbrg1	NP_001008140.1
Mus musculus (house mouse)	Tbrg1	NP_079565.2

Gene Ontology
Bibliography

Related articles in PubMed

Myst2/Kat7 histone acetyltransferase interaction proteomics reveals tumour-suppressor Niam as a novel binding partner in embryonic stem cells.
Pardo M, Yu L, Shen S, Tate P, Bode D, Letney BL, Quelle DE, Skarnes W, Choudhary JS
Scientific reports7(1)8157(2017 Aug)

Upregulation of ?3A Drives Homeostatic Plasticity by Rerouting AMPAR into the Recycling Endosomal Pathway.
Steinmetz CC, Tatavarty V, Sugino K, Shima Y, Joseph A, Lin H, Rutlin M, Lambo M, Hempel CM, Okaty BW, Paradis S, Nelson SB, Turrigiano GG
Cell reports16(10)2711-2722(2016 09)

NIAM-deficient mice are predisposed to the development of proliferative lesions including B-cell lymphomas.
Reed SM, Hagen J, Muniz VP, Rosean TR, Borcherding N, Sciegienka S, Goeken JA, Naumann PW, Zhang W, Tompkins VS, Janz S, Meyerholz DK, Quelle DE
PloS one9(11)e112126(2014)

A novel nuclear interactor of ARF and MDM2 (NIAM) that maintains chromosomal stability.
Tompkins VS, Hagen J, Frazier AA, Lushnikova T, Fitzgerald MP, di Tommaso A, Ladeveze V, Domann FE, Eischen CM, Quelle DE
The Journal of biological chemistry282(2)1322-33(2007 Jan)

Prediction of the coding sequences of mouse homologues of FLJ genes: the complete nucleotide sequences of 110 mouse FLJ-homologous cDnas identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, Kikuno R, Ohara R, Inamoto S, Koseki H, Hiraoka S, Saga Y, Kitamura H, Nakagawa T, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes11(2)127-35(2004 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NIAM down-regulation in vivo results in a significant predisposition to developing benign tumors or early stage cancers.
Title: NIAM-deficient mice are predisposed to the development of proliferative lesions including B-cell lymphomas.

novel protein with tumor suppressor-like behaviors and functional links to ARF-MDM2-p53 signaling
Title: A novel nuclear interactor of ARF and MDM2 (NIAM) that maintains chromosomal stability.

The following Tbrg1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tbrg1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu00207	NM_025289.3 Latest version!	Mus musculus transforming growth factor beta regulated gene 1 (Tbrg1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tbrg1 ( NM_025289.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_079565.2 Mus musculus transforming growth factor beta regulated gene 1 (Tbrg1), mRNA.

Price & Availability↑

CloneID	OMu00207
Clone ID Related Accession (Same CDS sequence)	NM_025289.3
Accession Version	NM_025289.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	transforming growth factor beta regulator 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC160125.2, BC065795.1 and BQ174234.1. On Mar 13, 2009 this sequence version replaced NM_025289.2. ##Evidence-Data-START## Transcript exon combination :: BC021331.1, BC043022.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGTGT TGAGCGGCCT GGCCTCCGAG CCGCGCACCC CGCTGTCCAG CAAGGCCAGG 
ATGAAAAGGC TCCCGAGGAA GAGCCAGAAC GAGAAGTACC GGCTGAAGTA CCTGCGGCTG 
CGCAGAGCAG CTAAGGCCAC GGTGTTTGAA AATGCTTCCA TCTGTGATGA AATTGCTCGT 
CTTGAGGAAA AATTTCTTAA AGCAAAGGAA GAAAGAAGAT ACTTGCTGAA GAAGCTCCTC 
CAGATACATG CTCTAACTGA AGGGGAGCCA CAGGCCGCTG CTCCTTCCCA CAGCTCCAGT 
TTGCCCCTGC CTTATGGTGT CACCAGCTCT GTGGGAACTA TGCAGGGAGC CGGGCCCAGC 
ACTGGGGCCG AGGAACCATT TGCGAAGAAA TCCAAGAAGG AGAAGAAAGA AAAGGGCAAG 
GAGAACAGCA AACTGGAAGT TCTGAAGAAA ACATCCAAGA AAAAGAAAAT GGAGGGAGGT 
GCTCGAAAAC TGGTTCGACC CATTGCCCTG GATCCTTCAG GACAGCCTGT GTTCCCCATC 
GGACTAGGGG GTCTGACAGT ATACAGTCTG GGGGAGATCA TCACCAACCG ACCTGGCTTC 
CATGATGAGA ATGCCATCTA TCCTGTGGGC TACTGCAGTA CCCGAGTGTA TGCTAGCATG 
AAGTGCCCAG ACCAGAAGTG TCTGTATACC TGTCAGATCA AGGACGGCGG TGTACAGCCT 
CAGTTTGAAA TTGTTCCTGA AGATGACCCC CAGAATACCA TCGTCGGCTC CTCTGCAGAT 
GCCTGTTATG AAGAACTGCT CAGGGCCATC AGTGCCACAA CAGGGAAACT GATGCCCAAC 
CCACTTTCAT GCGGTGCCGA CTTCTTTGGG TTTTCTCATC CAACCATCCA CAACCTGATT 
CAGAGTTGTC CAGAAGCTCA AAACTGTGTC AATTACCAGT GGGTGAAATT TGATGCGTGC 
AAACCCAGGA AGGGGCAGCT CTCTCAGGAA CTGCCAGAGA ATGATGCAAC TATGAGCCTT 
GAAGCCTTCC AGACACAGAC GTTTGATGAT GACCATGATG ATTCCATTCT GCCAGGCTCC 
CTGGACCTCC CTGAGCTTCA GCATGAAGCC TTTGTGTCAT CTTACCAGCC AGAGTTCCTG 
ACACACGAAC CCTTGGTTGA CACTGACCTG CAGCACCTGA AATCTCCATC CCAGTGTAGC 
CCAATTCAGT CTTCAGATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079565.2
CDS	169..1389
Translation	MSVLSGLASEPRTPLSSKARMKRLPRKSQNEKYRLKYLRLRRAAKATVFENASICDEIARLEEKFLKAKEERRYLLKKLLQIHALTEGEPQAAAPSHSSSLPLPYGVTSSVGTMQGAGPSTGAEEPFAKKSKKEKKEKGKENSKLEVLKKTSKKKKMEGGARKLVRPIALDPSGQPVFPIGLGGLTVYSLGEIITNRPGFHDENAIYPVGYCSTRVYASMKCPDQKCLYTCQIKDGGVQPQFEIVPEDDPQNTIVGSSADACYEELLRAISATTGKLMPNPLSCGADFFGFSHPTIHNLIQSCPEAQNCVNYQWVKFDACKPRKGQLSQELPENDATMSLEAFQTQTFDDDHDDSILPGSLDLPELQHEAFVSSYQPEFLTHEPLVDTDLQHLKSPSQCSPIQSSD

Target ORF information:

RefSeq Version	NM_025289.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus transforming growth factor beta regulated gene 1 (Tbrg1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025289.3

ORF Insert Sequence:

ATGAGCGTGT TGAGCGGCCT GGCCTCCGAG CCGCGCACCC CGCTGTCCAG CAAGGCCAGG 
ATGAAAAGGC TCCCGAGGAA GAGCCAGAAC GAGAAGTACC GGCTGAAGTA CCTGCGGCTG 
CGCAGAGCAG CTAAGGCCAC GGTGTTTGAA AATGCTTCCA TCTGTGATGA AATTGCTCGT 
CTTGAGGAAA AATTTCTTAA AGCAAAGGAA GAAAGAAGAT ACTTGCTGAA GAAGCTCCTC 
CAGATACATG CTCTAACTGA AGGGGAGCCA CAGGCCGCTG CTCCTTCCCA CAGCTCCAGT 
TTGCCCCTGC CTTATGGTGT CACCAGCTCT GTGGGAACTA TGCAGGGAGC CGGGCCCAGC 
ACTGGGGCCG AGGAACCATT TGCGAAGAAA TCCAAGAAGG AGAAGAAAGA AAAGGGCAAG 
GAGAACAGCA AACTGGAAGT TCTGAAGAAA ACATCCAAGA AAAAGAAAAT GGAGGGAGGT 
GCTCGAAAAC TGGTTCGACC CATTGCCCTG GATCCTTCAG GACAGCCTGT GTTCCCCATC 
GGACTAGGGG GTCTGACAGT ATACAGTCTG GGGGAGATCA TCACCAACCG ACCTGGCTTC 
CATGATGAGA ATGCCATCTA TCCTGTGGGC TACTGCAGTA CCCGAGTGTA TGCTAGCATG 
AAGTGCCCAG ACCAGAAGTG TCTGTATACC TGTCAGATCA AGGACGGCGG TGTACAGCCT 
CAGTTTGAAA TTGTTCCTGA AGATGACCCC CAGAATACCA TCGTCGGCTC CTCTGCAGAT 
GCCTGTTATG AAGAACTGCT CAGGGCCATC AGTGCCACAA CAGGGAAACT GATGCCCAAC 
CCACTTTCAT GCGGTGCCGA CTTCTTTGGG TTTTCTCATC CAACCATCCA CAACCTGATT 
CAGAGTTGTC CAGAAGCTCA AAACTGTGTC AATTACCAGT GGGTGAAATT TGATGCGTGC 
AAACCCAGGA AGGGGCAGCT CTCTCAGGAA CTGCCAGAGA ATGATGCAAC TATGAGCCTT 
GAAGCCTTCC AGACACAGAC GTTTGATGAT GACCATGATG ATTCCATTCT GCCAGGCTCC 
CTGGACCTCC CTGAGCTTCA GCATGAAGCC TTTGTGTCAT CTTACCAGCC AGAGTTCCTG 
ACACACGAAC CCTTGGTTGA CACTGACCTG CAGCACCTGA AATCTCCATC CCAGTGTAGC 
CCAATTCAGT CTTCAGATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Myst2/Kat7 histone acetyltransferase interaction proteomics reveals tumour-suppressor Niam as a novel binding partner in embryonic stem cells.
Scientific reports7(1)8157(2017 Aug)
Pardo M,Yu L,Shen S,Tate P,Bode D,Letney BL,Quelle DE,Skarnes W,Choudhary JS

Upregulation of ?3A Drives Homeostatic Plasticity by Rerouting AMPAR into the Recycling Endosomal Pathway.
Cell reports16(10)2711-2722(2016 09)
Steinmetz CC,Tatavarty V,Sugino K,Shima Y,Joseph A,Lin H,Rutlin M,Lambo M,Hempel CM,Okaty BW,Paradis S,Nelson SB,Turrigiano GG

NIAM-deficient mice are predisposed to the development of proliferative lesions including B-cell lymphomas.
PloS one9(11)e112126(2014)
Reed SM,Hagen J,Muniz VP,Rosean TR,Borcherding N,Sciegienka S,Goeken JA,Naumann PW,Zhang W,Tompkins VS,Janz S,Meyerholz DK,Quelle DE

A novel nuclear interactor of ARF and MDM2 (NIAM) that maintains chromosomal stability.
The Journal of biological chemistry282(2)1322-33(2007 Jan)
Tompkins VS,Hagen J,Frazier AA,Lushnikova T,Fitzgerald MP,di Tommaso A,Ladeveze V,Domann FE,Eischen CM,Quelle DE

Prediction of the coding sequences of mouse homologues of FLJ genes: the complete nucleotide sequences of 110 mouse FLJ-homologous cDnas identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
DNA research : an international journal for rapid publication of reports on genes and genomes11(2)127-35(2004 Apr)
Okazaki N,Kikuno R,Ohara R,Inamoto S,Koseki H,Hiraoka S,Saga Y,Kitamura H,Nakagawa T,Nagase T,Ohara O,Koga H