Tacr3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tacr3
Entrez Gene ID	21338
Full Name	tachykinin receptor 3
Synonyms	Nk3r,Nkr,Tac3r
General protein information	Preferred Names tachykinin receptor 3 Names neuromedin-K receptor NK-3 receptor Nk3 receptor neurokinin 3 receptor neurokinin B receptor neuromedin K receptor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 G3
Summary	This gene belongs to a family of genes that function as receptors for tachykinins. The receptors belonging to this family are characterized by interactions with G proteins and 7 hydrophobic transmembrane regions. This gene encodes the receptor for the tachykinin neurokinin 3, also referred to as neurokinin B. [provided by RefSeq, Mar 2010].

mRNA and Protein(s)

mRNA	Protein	Name
NM_021382.6	NP_067357.1	neuromedin-K receptor

Pathways from BioSystems

Homologies

	TACR3	NP_001032951.1
Canis lupus familiaris (dog)	TACR3	NP_001091010.1
Gallus gallus (chicken)	TACR3	XP_001232174.2
Anopheles gambiae (African malaria mosquito)	GPRTAK2	XP_321512.5
Caenorhabditis elegans (roundworm)	C49A9.7	NP_500930.1
Bos taurus (cattle)	TACR3	NP_001179262.1
Danio rerio (zebrafish)	tacr3l	NP_001243564.1
Drosophila melanogaster (fruit fly)	Takr99D	NP_001163772.1
Homo sapiens (human)	TACR3	NP_001050.1
Xenopus tropicalis (tropical clawed frog)	tacr3	XP_002934808.1
Mus musculus (house mouse)	Tacr3	NP_067357.1
Rattus norvegicus (Norway rat)	Tacr3	NP_058749.1
Pan troglodytes (chimpanzee)	TACR3	XP_003950435.1
Danio rerio (zebrafish)	tacr3a	NP_001243567.1

Gene Ontology
Bibliography

Related articles in PubMed

Lef1-dependent hypothalamic neurogenesis inhibits anxiety.
Xie Y, Kaufmann D, Moulton MJ, Panahi S, Gaynes JA, Watters HN, Zhou D, Xue HH, Fung CM, Levine EM, Letsou A, Brennan KC, Dorsky RI
PLoS biology15(8)e2002257(2017 Aug)

Targeting neurokinin-3 receptor: a novel anti-angiogenesis strategy for cancer treatment.
Wang T, Chen S, Wang S, Shi L, Wang C, Zhang J, Gao Y, Li G, Qi Y, An X, Chen L
Oncotarget8(25)40713-40723(2017 Jun)

Purification and Transcriptomic Analysis of Mouse Fetal Leydig Cells Reveals Candidate Genes for Specification of Gonadal Steroidogenic Cells.
McClelland KS, Bell K, Larney C, Harley VR, Sinclair AH, Oshlack A, Koopman P, Bowles J
Biology of reproduction92(6)145(2015 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that activation of neurokinin 3 and kappa-opioid receptors (NK3R and KOR) excites and inhibits kisspeptin, neurokinin B (NKB), and dynorphin (KNDy neurons).
Title: Regulation of arcuate neurons coexpressing kisspeptin, neurokinin B, and dynorphin by modulators of neurokinin 3 and ?-opioid receptors in adult male mice.

although capable of fertility, Tacr3-deficient mice have central reproductive defects
Title: Uncovering novel reproductive defects in neurokinin B receptor null mice: closing the gap between mice and men.

NKB-NK3R signaling plays a role in pubertal maturation; its alterations may contribute to pubertal disorders linked to metabolic stress and negative energy balance.
Title: Role of neurokinin B in the control of female puberty and its modulation by metabolic status.

neurokinin 3 receptor activation in the median preoptic nucleus modulates body temperature
Title: Activation of neurokinin 3 receptors in the median preoptic nucleus decreases core temperature in the rat.

Together, these results support a role for the NK3 receptor in reactivity to dopaminergic stimuli, but the lack of robust changes indicates that the sensitivity to dopamine may be activity-dependent or benign in nature.
Title: Characterization of behavioral response to amphetamine, tyrosine hydroxylase levels, and dopamine receptor levels in neurokinin 3 receptor knockout mice.

The following Tacr3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tacr3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu12268	NM_021382.6 Latest version!	Mus musculus tachykinin receptor 3 (Tacr3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tacr3 ( NM_021382.6 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067357.1 Mus musculus tachykinin receptor 3 (Tacr3), mRNA.

Price & Availability↑

CloneID	OMu12268
Clone ID Related Accession (Same CDS sequence)	NM_021382.6
Accession Version	NM_021382.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	neuromedin-K receptor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK031898.1, AC101889.6 and AV336806.1. On Mar 13, 2010 this sequence version replaced NM_021382.5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK031898.1, BC066845.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849389 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTCGG TTCCCACCGG CGAAAACTGG ACGGACGGGA CGGCGGGCGT GGGGTCCCAT 
ACAGGGAATC TGAGCGCTGC GCTGGGGATC ACTGAGTGGC TTGCGCTCCA GGCTGGCAAC 
TTCTCCTCCG CGCTGGGCTT GCCAGTGACA TCCCAGGCAC CTTCCCAAGT CCGGGCCAAC 
CTGACGAACC AGTTCGTGCA GCCCTCCTGG CGAATCGCGC TCTGGTCGCT GGCCTATGGC 
TTAGTGGTGG CGGTGGCAGT CTTCGGAAAC CTCATCGTTA TTTGGATCAT CTTGGCCCAC 
AAGCGCATGA GGACCGTCAC CAACTATTTC CTGGTAAACC TGGCTTTCTC CGACGCCTCC 
GTGGCTGCCT TCAACACCTT GGTCAATTTC ATCTATGGTG TTCACAGCGA GTGGTACTTT 
GGCGCCAACT ACTGCCGCTT CCAGAACTTC TTTCCCATCA CAGCGGTGTT TGCCAGCATC 
TACTCTATGA CAGCCATTGC AGTGGACAGG TATATGGCCA TTATCGATCC TTTGAAACCC 
AGACTATCTG CCACAGCCAC TAAGATTGTC ATCGGAAGTA TTTGGATTTT GGCATTTCTA 
CTTGCCTTCC CTCAATGTCT TTATTCCAAA ATAAAAGTCA TGCCAGGCCG TACCCTTTGC 
TATGTGCAGT GGCCAGAAGG TCCCAAGCAA CATTTCACGT ACCACATCAT TGTTATCATC 
CTGGTGTACT GTTTCCCATT GCTCATCATG GGTGTCACCT ACACCATCGT TGGAATTACT 
CTCTGGGGAG GAGAGATCCC AGGAGACACC TGTGACAAGT ACCATGAGCA GCTTAAGGCT 
AAACGAAAGG TTGTAAAAAT GATGATTATT GTGGTGGTGA CATTTGCCAT CTGCTGGCTA 
CCCTACCATG TGTATTTCAT TCTCACTGCG ATCTACCAAC AGTTAAACAG GTGGAAATAC 
ATCCAGCAGG TCTACCTGGC TAGCTTCTGG CTGGCCATGA GCTCAACCAT GTACAACCCC 
ATCATCTACT GCTGTTTGAA CAAAAGATTT CGTGCAGGCT TCAAGAGAGC ATTTCGCTGG 
TGTCCTTTCA TCCAAGTCTC CAGCTACGAT GAGCTGGAGC TCAAGACCAC CAGGTTTCAT 
CCCACACGGC AGAGCAGCCT GTACACCGTG AGCAGGATGG AGTCAGTGAC CGTGCTGTAT 
GACCCCAGTG AAGGGGACCC GGCCAAGTCC AGCCGCAAGA AGAGGGCGGT GCCCAGAGAC 
CCCAGTGCCA ATGGCTGCTC CCACAGAGAG TTCAAATCTG CCTCCACCAC ATCAAGTTTC 
ATAAGCTCGC CCTATACCTC TGTGGATGAA TATTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067357.1
CDS	267..1625
Translation	MASVPTGENWTDGTAGVGSHTGNLSAALGITEWLALQAGNFSSALGLPVTSQAPSQVRANLTNQFVQPSWRIALWSLAYGLVVAVAVFGNLIVIWIILAHKRMRTVTNYFLVNLAFSDASVAAFNTLVNFIYGVHSEWYFGANYCRFQNFFPITAVFASIYSMTAIAVDRYMAIIDPLKPRLSATATKIVIGSIWILAFLLAFPQCLYSKIKVMPGRTLCYVQWPEGPKQHFTYHIIVIILVYCFPLLIMGVTYTIVGITLWGGEIPGDTCDKYHEQLKAKRKVVKMMIIVVVTFAICWLPYHVYFILTAIYQQLNRWKYIQQVYLASFWLAMSSTMYNPIIYCCLNKRFRAGFKRAFRWCPFIQVSSYDELELKTTRFHPTRQSSLYTVSRMESVTVLYDPSEGDPAKSSRKKRAVPRDPSANGCSHREFKSASTTSSFISSPYTSVDEYS

Target ORF information:

RefSeq Version	NM_021382.6
Organism	Mus musculus(house mouse)
Definition	Mus musculus tachykinin receptor 3 (Tacr3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021382.6

ORF Insert Sequence:

ATGGCCTCGG TTCCCACCGG CGAAAACTGG ACGGACGGGA CGGCGGGCGT GGGGTCCCAT 
ACAGGGAATC TGAGCGCTGC GCTGGGGATC ACTGAGTGGC TTGCGCTCCA GGCTGGCAAC 
TTCTCCTCCG CGCTGGGCTT GCCAGTGACA TCCCAGGCAC CTTCCCAAGT CCGGGCCAAC 
CTGACGAACC AGTTCGTGCA GCCCTCCTGG CGAATCGCGC TCTGGTCGCT GGCCTATGGC 
TTAGTGGTGG CGGTGGCAGT CTTCGGAAAC CTCATCGTTA TTTGGATCAT CTTGGCCCAC 
AAGCGCATGA GGACCGTCAC CAACTATTTC CTGGTAAACC TGGCTTTCTC CGACGCCTCC 
GTGGCTGCCT TCAACACCTT GGTCAATTTC ATCTATGGTG TTCACAGCGA GTGGTACTTT 
GGCGCCAACT ACTGCCGCTT CCAGAACTTC TTTCCCATCA CAGCGGTGTT TGCCAGCATC 
TACTCTATGA CAGCCATTGC AGTGGACAGG TATATGGCCA TTATCGATCC TTTGAAACCC 
AGACTATCTG CCACAGCCAC TAAGATTGTC ATCGGAAGTA TTTGGATTTT GGCATTTCTA 
CTTGCCTTCC CTCAATGTCT TTATTCCAAA ATAAAAGTCA TGCCAGGCCG TACCCTTTGC 
TATGTGCAGT GGCCAGAAGG TCCCAAGCAA CATTTCACGT ACCACATCAT TGTTATCATC 
CTGGTGTACT GTTTCCCATT GCTCATCATG GGTGTCACCT ACACCATCGT TGGAATTACT 
CTCTGGGGAG GAGAGATCCC AGGAGACACC TGTGACAAGT ACCATGAGCA GCTTAAGGCT 
AAACGAAAGG TTGTAAAAAT GATGATTATT GTGGTGGTGA CATTTGCCAT CTGCTGGCTA 
CCCTACCATG TGTATTTCAT TCTCACTGCG ATCTACCAAC AGTTAAACAG GTGGAAATAC 
ATCCAGCAGG TCTACCTGGC TAGCTTCTGG CTGGCCATGA GCTCAACCAT GTACAACCCC 
ATCATCTACT GCTGTTTGAA CAAAAGATTT CGTGCAGGCT TCAAGAGAGC ATTTCGCTGG 
TGTCCTTTCA TCCAAGTCTC CAGCTACGAT GAGCTGGAGC TCAAGACCAC CAGGTTTCAT 
CCCACACGGC AGAGCAGCCT GTACACCGTG AGCAGGATGG AGTCAGTGAC CGTGCTGTAT 
GACCCCAGTG AAGGGGACCC GGCCAAGTCC AGCCGCAAGA AGAGGGCGGT GCCCAGAGAC 
CCCAGTGCCA ATGGCTGCTC CCACAGAGAG TTCAAATCTG CCTCCACCAC ATCAAGTTTC 
ATAAGCTCGC CCTATACCTC TGTGGATGAA TATTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lef1-dependent hypothalamic neurogenesis inhibits anxiety.
PLoS biology15(8)e2002257(2017 Aug)
Xie Y,Kaufmann D,Moulton MJ,Panahi S,Gaynes JA,Watters HN,Zhou D,Xue HH,Fung CM,Levine EM,Letsou A,Brennan KC,Dorsky RI

Targeting neurokinin-3 receptor: a novel anti-angiogenesis strategy for cancer treatment.
Oncotarget8(25)40713-40723(2017 Jun)
Wang T,Chen S,Wang S,Shi L,Wang C,Zhang J,Gao Y,Li G,Qi Y,An X,Chen L

Purification and Transcriptomic Analysis of Mouse Fetal Leydig Cells Reveals Candidate Genes for Specification of Gonadal Steroidogenic Cells.
Biology of reproduction92(6)145(2015 Jun)
McClelland KS,Bell K,Larney C,Harley VR,Sinclair AH,Oshlack A,Koopman P,Bowles J