Dnd1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dnd1
Entrez Gene ID	213236
Full Name	DND microRNA-mediated repression inhibitor 1
Synonyms	BC034897,RBMS4,Ter
General protein information	Preferred Names DND microRNA-mediated repression inhibitor 1 Names dead end protein homolog 1 RNA-binding motif, single-stranded-interacting protein 4 dead end homolog 1 teratoma
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18 B2\|18 19.46 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_173383.2	NP_775559.2	dead end protein homolog 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Dnd1	NP_001102849.1
Homo sapiens (human)	DND1	NP_919225.1
Gallus gallus (chicken)	DND1	XP_423051.4
Bos taurus (cattle)	DND1	NP_001180074.1
Mus musculus (house mouse)	Dnd1	NP_775559.2
Xenopus tropicalis (tropical clawed frog)	dnd1	NP_001037899.1
Pan troglodytes (chimpanzee)	DND1	XP_517978.2
Canis lupus familiaris (dog)	DND1	XP_848834.1

Gene Ontology
Bibliography

Related articles in PubMed

Do Gametes Woo? Evidence for Their Nonrandom Union at Fertilization.
Nadeau JH
Genetics207(2)369-387(2017 Oct)

DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs.
Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, Garzia A, Morozov P, Manickavel S, McFarland HL, Roeder RG, Hafner M, Tuschl T
Nature543(7646)568-572(2017 03)

Dead end1 is an essential partner of NANOS2 for selective binding of target RNAs in male germ cell development.
Suzuki A, Niimi Y, Shinmyozu K, Zhou Z, Kiso M, Saga Y
EMBO reports17(1)37-46(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DND1 binds a UU(A/U) trinucleotide motif predominantly in the 3' untranslated regions of mRNA, and destabilizes target mRNAs through direct recruitment of the CCR4-NOT deadenylase complex.
Title: DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs.

DND1 interacts with NANOS2 to load unique RNAs into the CCR4-NOTdeaden complex; this interaction is mediated by the zinc finger domain of NANOS2, which is essential for its association with target RNAs; DND1 is an essential partner for NANOS2 that leads to the degradation of specific RNAs in male germ cell development
Title: Dead end1 is an essential partner of NANOS2 for selective binding of target RNAs in male germ cell development.

Dnd1 heterozygotes, lower oxygen availability was associated with metabolic differences
Title: Left-Biased Spermatogenic Failure in 129/SvJ Dnd1Ter/+ Mice Correlates with Differences in Vascular Architecture, Oxygen Availability, and Metabolites.

APOBEC3G inhibits DND1 function to regulate microRNA activity.
Title: APOBEC3 inhibits DEAD-END function to regulate microRNA activity.

results show that Dnd1 is essential for normal allelic inheritance and that Dnd1Ter has a novel combination of functions that significantly increase risk for both testicular and intestinal cancer
Title: Contrasting effects of Deadend1 (Dnd1) gain and loss of function mutations on allelic inheritance, testicular cancer, and intestinal polyposis.

The following Dnd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dnd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu04526	NM_173383.2 Latest version!	Mus musculus DND microRNA-mediated repression inhibitor 1 (Dnd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dnd1 ( NM_173383.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_775559.2 Mus musculus DND microRNA-mediated repression inhibitor 1 (Dnd1), mRNA.

Price & Availability↑

CloneID	OMu04526
Clone ID Related Accession (Same CDS sequence)	NM_173383.2
Accession Version	NM_173383.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	dead end protein homolog 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC034897.1 and AY321066.1. On Oct 1, 2008 this sequence version replaced NM_173383.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY321066.1, SRR1660825.222158.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164143 [ECO:0000348] ##Evidence-Data-END##

ATGCAGTCCA AACGGGAGTG CGAGCAATGG TGTGAGAGGG TGAATCCAGA GAACAAGGCA 
GCCCTGGAGG CGTGGGTAAG GGAGACTGGC ATCCGCCTGG TGCAGGTCAA CGGGCAAAGG 
AAGTATGGAG GGCCACCCCC AGGCTGGGTG GGCAGCCCGC CACCGTCAGG GTCCGAGGTG 
TATATCGGAC GACTTCCGCA GGACGTGTAC GAGCACCAGC TAATCCCACT CTTCCAGCGC 
GTGGGGCGCC TCTATGAGTT CCGCCTAATG ATGACCTTCA GTGGTTTGAA CCGCGGCTTT 
GCCTATGCGC GCTACAGCTC GCGGCGCGGT GCCCAGGCCG CCATTGCTAC GCTGCACAAC 
CACCAGCTGC GGCCTTCTTG CCAGCTCCTG GTGTGCCGAA GCACCGAGAA GTGCGAGCTG 
ACGGTGGACG GGCTGCCTCT TAGCTTAAAC CGCCGAGCGT TACTGCTCGC CCTGCAACCG 
TTCGGTCCCT GCCTCCAGGA GACACTGCTG CTACCCAGCC CTGGGTCTGC GCCCTCTCAG 
ATTGCGCTGC TCAAGTTCAG TACGCACCGA GCTGCTGCTA TGGCCAAAAA GGCCCTGGTA 
GAAGGTCAGT CACGCCTCTG TGGAGAGCAG GTGGCTGTGG AGTGGCTCAA GCCAGACCTA 
AAGCAGCACT TCCGCCAGCA GCTTGCAGGT CCCTCACTGC GGTTCCTCAG GCCAGATGTC 
AGCCAACTAA CCCAGACCAG GGAGAAGCTG GGGTCCCAAG GGGCTCGGGC TGCCCTGCAA 
CTGCTGTGTC AACGGATGAA GCTAGGCAGC CCAGTGTTCC TGACCAAGTG TTTAGGAACA 
GGGCCTGCTG GCTGGCATCG CTTTTGGTAC CAGGTGGTGA TCCCTGGGCA TCCAGTGCCT 
TTCAGTGGCC TCATTTGGGT TGTGTTGGCC TCTGATTGGC AGGATGGGCA TGAGGTGGCC 
AAGGATGCTG TGTCCGCACA GCTGCTGGAG GCACTGAGTG AACCTAGGAC CAGCTTGTGG 
TCTCCTGGGG CTGAAGCAGG TACTATGGTT AAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775559.2
CDS	49..1107
Translation	MQSKRECEQWCERVNPENKAALEAWVRETGIRLVQVNGQRKYGGPPPGWVGSPPPSGSEVYIGRLPQDVYEHQLIPLFQRVGRLYEFRLMMTFSGLNRGFAYARYSSRRGAQAAIATLHNHQLRPSCQLLVCRSTEKCELTVDGLPLSLNRRALLLALQPFGPCLQETLLLPSPGSAPSQIALLKFSTHRAAAMAKKALVEGQSRLCGEQVAVEWLKPDLKQHFRQQLAGPSLRFLRPDVSQLTQTREKLGSQGARAALQLLCQRMKLGSPVFLTKCLGTGPAGWHRFWYQVVIPGHPVPFSGLIWVVLASDWQDGHEVAKDAVSAQLLEALSEPRTSLWSPGAEAGTMVKQ

Target ORF information:

RefSeq Version	NM_173383.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus DND microRNA-mediated repression inhibitor 1 (Dnd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173383.2

ORF Insert Sequence:

ATGCAGTCCA AACGGGAGTG CGAGCAATGG TGTGAGAGGG TGAATCCAGA GAACAAGGCA 
GCCCTGGAGG CGTGGGTAAG GGAGACTGGC ATCCGCCTGG TGCAGGTCAA CGGGCAAAGG 
AAGTATGGAG GGCCACCCCC AGGCTGGGTG GGCAGCCCGC CACCGTCAGG GTCCGAGGTG 
TATATCGGAC GACTTCCGCA GGACGTGTAC GAGCACCAGC TAATCCCACT CTTCCAGCGC 
GTGGGGCGCC TCTATGAGTT CCGCCTAATG ATGACCTTCA GTGGTTTGAA CCGCGGCTTT 
GCCTATGCGC GCTACAGCTC GCGGCGCGGT GCCCAGGCCG CCATTGCTAC GCTGCACAAC 
CACCAGCTGC GGCCTTCTTG CCAGCTCCTG GTGTGCCGAA GCACCGAGAA GTGCGAGCTG 
ACGGTGGACG GGCTGCCTCT TAGCTTAAAC CGCCGAGCGT TACTGCTCGC CCTGCAACCG 
TTCGGTCCCT GCCTCCAGGA GACACTGCTG CTACCCAGCC CTGGGTCTGC GCCCTCTCAG 
ATTGCGCTGC TCAAGTTCAG TACGCACCGA GCTGCTGCTA TGGCCAAAAA GGCCCTGGTA 
GAAGGTCAGT CACGCCTCTG TGGAGAGCAG GTGGCTGTGG AGTGGCTCAA GCCAGACCTA 
AAGCAGCACT TCCGCCAGCA GCTTGCAGGT CCCTCACTGC GGTTCCTCAG GCCAGATGTC 
AGCCAACTAA CCCAGACCAG GGAGAAGCTG GGGTCCCAAG GGGCTCGGGC TGCCCTGCAA 
CTGCTGTGTC AACGGATGAA GCTAGGCAGC CCAGTGTTCC TGACCAAGTG TTTAGGAACA 
GGGCCTGCTG GCTGGCATCG CTTTTGGTAC CAGGTGGTGA TCCCTGGGCA TCCAGTGCCT 
TTCAGTGGCC TCATTTGGGT TGTGTTGGCC TCTGATTGGC AGGATGGGCA TGAGGTGGCC 
AAGGATGCTG TGTCCGCACA GCTGCTGGAG GCACTGAGTG AACCTAGGAC CAGCTTGTGG 
TCTCCTGGGG CTGAAGCAGG TACTATGGTT AAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Do Gametes Woo? Evidence for Their Nonrandom Union at Fertilization.
Genetics207(2)369-387(2017 Oct)
Nadeau JH

DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs.
Nature543(7646)568-572(2017 03)
Yamaji M,Jishage M,Meyer C,Suryawanshi H,Der E,Yamaji M,Garzia A,Morozov P,Manickavel S,McFarland HL,Roeder RG,Hafner M,Tuschl T

Dead end1 is an essential partner of NANOS2 for selective binding of target RNAs in male germ cell development.
EMBO reports17(1)37-46(2016 Jan)
Suzuki A,Niimi Y,Shinmyozu K,Zhou Z,Kiso M,Saga Y