Zfp748 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Zfp748
Entrez Gene ID	212276
Full Name	zinc finger protein 748
Synonyms	KRAB-O,Rslcan20/21,Zfp208,mszf54
General protein information	Preferred Names zinc finger protein 748 Names zinc finger protein 748 KRAB zinc finger protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13\|13 B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001035231.3	NP_001030308.1	zinc finger protein 748

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Zfp748	NP_001030308.1
Rattus norvegicus (Norway rat)	LOC102549842	XP_006227879.1

Gene Ontology
Bibliography

Related articles in PubMed

Epigenetic gene silencing by the SRY protein is mediated by a KRAB-O protein that recruits the KAP1 co-repressor machinery.
Peng H, Ivanov AV, Oh HJ, Lau YF, Rauscher FJ 3rd
The Journal of biological chemistry284(51)35670-80(2009 Dec)

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

Functional analysis of the SRY-KRAB interaction in mouse sex determination.
Polanco JC, Wilhelm D, Mizusaki H, Jackson A, Browne C, Davidson T, Harley V, Sinclair A, Koopman P
Biology of the cell101(1)55-67(2009 Jan)

Sry associates with the heterochromatin protein 1 complex by interacting with a KRAB domain protein.
Oh HJ, Li Y, Lau YF
Biology of reproduction72(2)407-15(2005 Feb)

Regulator of sex-limitation (Rsl) encodes a pair of KRAB zinc-finger genes that control sexually dimorphic liver gene expression.
Krebs CJ, Larkins LK, Price R, Tullis KM, Miller RD, Robins DM
Genes & development17(21)2664-74(2003 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Reduced Sox9 expression in KRAB-knockdown cells supports a role for KRAB-O and perhaps other KRAB genes in mediating SRY function
Title: Functional analysis of the SRY-KRAB interaction in mouse sex determination.

The following Zfp748 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Zfp748 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu12801	NM_001035231.3 Latest version!	Mus musculus zinc finger protein 748 (Zfp748), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Zfp748 ( NM_001035231.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001030308.1 Mus musculus zinc finger protein 748 (Zfp748), mRNA.

Price & Availability↑

CloneID	OMu12801
Clone ID Related Accession (Same CDS sequence)	NM_001035231.3
Accession Version	NM_001035231.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-25
Organism	Mus musculus(house mouse)
Product	zinc finger protein 748
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CN460452.1, AK132196.1 and BC055310.1. On Oct 30, 2013 this sequence version replaced NM_001035231.2. ##Evidence-Data-START## Transcript exon combination :: AK132196.1, AK220562.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGAAA TGCTGTCATT CAGGGATGTG GCCGTTGATT TCTCTGCAGA GGAGTGGGAG 
TGCCTGGAGC CTGCTCAGTG GAATCTGTAC AGGGACGTGA TGCTGGAGAA TTACAGCCAC 
CTTGTGTTCC TGGGTCTTGC TTTCTCTAAG CCATACCTGG TCACGTTTCT GGAGCAAAGA 
CAAGAGCCTT TGAATGAGAA GAGACAAGTG GCCACCACCA TGCACCCAGA TATAAAACCA 
TATAAATGTA AAGAATGTGG CAAAGCCTGT GATTGGAACT CACTCCTTCT CGAACACCAG 
AGAACCAATC CAGGAGAGAA TGCCTACAAG TGTGAAGAAT GTGGTCAGGC TTCTGGCTCT 
TACTCAGTAA TTCCTGAACA CCATATAAAC GATACGGAAG AGAAAGCCTG CAAGTGTGAA 
GAATGTGGTA AAGTCATTTG TACTTGCTCA GAAAATTCTA GCTACCAGAG AATTTGCATT 
GGAGAAAACC CCTACAAGTG TGAAGAATGT GGAAAAGCCT TTTCTTCTCA TTCATGTCTT 
GCTCAACATG AGGTAGAACA CACTGGACAG CAATTTTATA ACTGTGAAGA ATGTGGGAAA 
CTGTTTTATT GTCCTTCTCA CCTTACAGAA CATCAAAAAA TTCATTCTCA AGAGAATCTC 
TTTAAGATTG AGGTATGTAG CGAGGTCTTT TGTGCTCCCA TAGAACTTTC TAAAGACCAG 
AACTTTTGTA CTGAAGAAAA ACCCTACAGG TATGAAGACT ATGTTAAGGC CTTTAGCGCT 
TGCTCAGTTC TTTCTGAACA CCCGACAATT CATCCAGGAG AGAAAGCATT CAAGTGTGAA 
GAATGTGGCA ATGCCTTTTG TACACTCCAT TCAGTCTCTA AAATGAAAAT TCATTATGAA 
GTGAAATCCT ACAAGTGTGA AGAGTGTGGG AAAGCCTTTG CTACTCATCT TTCCCTGATT 
CAGCATAAGA TAGGTCATAC TCGAGAGAAA CCTTATCAAT GTGAAGAATG TGGCAAAATG 
TTTTACTGTT CTTCAAACCT TAAGCAACAT CAAATAACTC ATTCTCAAGA GAAACCCTAC 
AAGTGTGAAG TATGTGGCAA GGTCTTTAGA ACTTGCTGGC AACTTTCTAA ACACCTGAGA 
ATCCATTCTG GAGAGAAACC ATACAAGTGT GAGGAATGTG GCAAAGCCTT TTATACTCTC 
TCCTACCTTA CTCAGCACAA ATTAGGTCAC ACTGGGGAGA AACCTTACAA ATGTGAGGAA 
TGTGGCAAAA CCTTTTACTA CCCTTCAGTC CTTAAGGAAC ACCTAGCAAT TCATTCTGGA 
GAGAAACCGT ACAGGTGTGA TGAATGTGGC AAGGACTTTT GTACTCGCTC AGGACGTTCC 
AGACACCAGA GAATCCATAC TGGAGAGAAA CCGTACAAGT GTGAGCAATG TGGAAAGGCC 
TTCAGTACCC ATTCATACCT TTCTCATCAC AAGATAGTTC ACACTGGACA TAAACCCTAC 
AAGTGTGAAG AGTGTGGCAA AAAATTTTAT TATCCTTCTC GACTTAAGGA GCATCAAAGG 
ATTCATTCTC AAGAGAATCC CTACAAGTGT GAAATATGTG GCAAAGCCTT TTATACTCAC 
TCATACTTTA CCCAGCACAA ATTAGGTCAC ACTGGGGAGA AACCTTACAA ATGTGAGGAA 
TGTGGCAAAA CCTTTTACTA CCCTTCAATC CTTAAGGAAC ACCTAGCAAT TCATTCTGGA 
AAGAAACCGT ACAGGTGTGA AGAATGTGGC AAGGACTTTT GTACTCGCTC AGGACGTTCC 
AGACACCAAA GAATCCATAC TGGAGAGAAA CCCCACAAGT GTGAAGAATG CGGAAAAGTC 
TTTTCTACTC ATTCATATCT TACTCAGCAC AAAGTAGTCC ACTCTGGAGA GAAGCCCTAC 
AAGTGTGAAG AGTGTGGCAA AAAGTTTTAC TATCCTTCTC GCCTTAAGGA GCATCAAAGG 
ATTCATTCTC AAGAGAATCC TTACAAGTGT GAAATATGTG GCAATGTATT TTGTACTCCC 
AAAGGACTTT CGAAACACCA GAGATTTCAT ACTGGAGAGA AACCCTACAA GTGTGAAGAA 
TGCGGGAAAA TGTTTTATTA TCCTTCTCGA CTTAAGGAAC ATCAAAGAAT TCATTCTCAA 
GAGAATCCTT ACAAGTGTGA AATATGTGGC AAAGCCTTTT ATACTCACTC CTACCTTACT 
CAGCACAAAT TAGGTCATAC TGGGGAGAAA CCTTACAAAT GTGAGGAATG TGGCAAAACC 
TTTTACTACC CTTCAATCCT TAAGGAACAC CTAGCAATTC ATTCTGGAAA GAAACCGTAC 
AGGTGTGAAG AATGTGGCAA GGACTTTTGT ACTCGCTCAG GACGTTCCAG ACACCAGAGA 
ATCCATACTG GAGAGAAACC CTACAAGTGT GAGCAATGTG GAAAGGCCTT CAGTACTCAC 
TCATACCTTT CTCAACACAA GGTAGTCCAC TCTGGAGAGA AGCCCTACAA GTGTGAAGAG 
TGTGGCAAAA TGTTTTACTA TTCTTCTCGA CTTAAGGAGC ATCAAAGGAT TCATTCTCAA 
GAGAATCCCT ACAAGTGTGA AGTGTGTGGC AAAGTCTTTA GTGCTCACTT AGAACTTGCT 
ACCCACCTAA GTATTCATTC CGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001030308.1
CDS	117..2783
Translation	MEEMLSFRDVAVDFSAEEWECLEPAQWNLYRDVMLENYSHLVFLGLAFSKPYLVTFLEQRQEPLNEKRQVATTMHPDIKPYKCKECGKACDWNSLLLEHQRTNPGENAYKCEECGQASGSYSVIPEHHINDTEEKACKCEECGKVICTCSENSSYQRICIGENPYKCEECGKAFSSHSCLAQHEVEHTGQQFYNCEECGKLFYCPSHLTEHQKIHSQENLFKIEVCSEVFCAPIELSKDQNFCTEEKPYRYEDYVKAFSACSVLSEHPTIHPGEKAFKCEECGNAFCTLHSVSKMKIHYEVKSYKCEECGKAFATHLSLIQHKIGHTREKPYQCEECGKMFYCSSNLKQHQITHSQEKPYKCEVCGKVFRTCWQLSKHLRIHSGEKPYKCEECGKAFYTLSYLTQHKLGHTGEKPYKCEECGKTFYYPSVLKEHLAIHSGEKPYRCDECGKDFCTRSGRSRHQRIHTGEKPYKCEQCGKAFSTHSYLSHHKIVHTGHKPYKCEECGKKFYYPSRLKEHQRIHSQENPYKCEICGKAFYTHSYFTQHKLGHTGEKPYKCEECGKTFYYPSILKEHLAIHSGKKPYRCEECGKDFCTRSGRSRHQRIHTGEKPHKCEECGKVFSTHSYLTQHKVVHSGEKPYKCEECGKKFYYPSRLKEHQRIHSQENPYKCEICGNVFCTPKGLSKHQRFHTGEKPYKCEECGKMFYYPSRLKEHQRIHSQENPYKCEICGKAFYTHSYLTQHKLGHTGEKPYKCEECGKTFYYPSILKEHLAIHSGKKPYRCEECGKDFCTRSGRSRHQRIHTGEKPYKCEQCGKAFSTHSYLSQHKVVHSGEKPYKCEECGKMFYYSSRLKEHQRIHSQENPYKCEVCGKVFSAHLELATHLSIHSG

Target ORF information:

RefSeq Version	NM_001035231.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus zinc finger protein 748 (Zfp748), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001035231.3

ORF Insert Sequence:

ATGGAGGAAA TGCTGTCATT CAGGGATGTG GCCGTTGATT TCTCTGCAGA GGAGTGGGAG 
TGCCTGGAGC CTGCTCAGTG GAATCTGTAC AGGGACGTGA TGCTGGAGAA TTACAGCCAC 
CTTGTGTTCC TGGGTCTTGC TTTCTCTAAG CCATACCTGG TCACGTTTCT GGAGCAAAGA 
CAAGAGCCTT TGAATGAGAA GAGACAAGTG GCCACCACCA TGCACCCAGA TATAAAACCA 
TATAAATGTA AAGAATGTGG CAAAGCCTGT GATTGGAACT CACTCCTTCT CGAACACCAG 
AGAACCAATC CAGGAGAGAA TGCCTACAAG TGTGAAGAAT GTGGTCAGGC TTCTGGCTCT 
TACTCAGTAA TTCCTGAACA CCATATAAAC GATACGGAAG AGAAAGCCTG CAAGTGTGAA 
GAATGTGGTA AAGTCATTTG TACTTGCTCA GAAAATTCTA GCTACCAGAG AATTTGCATT 
GGAGAAAACC CCTACAAGTG TGAAGAATGT GGAAAAGCCT TTTCTTCTCA TTCATGTCTT 
GCTCAACATG AGGTAGAACA CACTGGACAG CAATTTTATA ACTGTGAAGA ATGTGGGAAA 
CTGTTTTATT GTCCTTCTCA CCTTACAGAA CATCAAAAAA TTCATTCTCA AGAGAATCTC 
TTTAAGATTG AGGTATGTAG CGAGGTCTTT TGTGCTCCCA TAGAACTTTC TAAAGACCAG 
AACTTTTGTA CTGAAGAAAA ACCCTACAGG TATGAAGACT ATGTTAAGGC CTTTAGCGCT 
TGCTCAGTTC TTTCTGAACA CCCGACAATT CATCCAGGAG AGAAAGCATT CAAGTGTGAA 
GAATGTGGCA ATGCCTTTTG TACACTCCAT TCAGTCTCTA AAATGAAAAT TCATTATGAA 
GTGAAATCCT ACAAGTGTGA AGAGTGTGGG AAAGCCTTTG CTACTCATCT TTCCCTGATT 
CAGCATAAGA TAGGTCATAC TCGAGAGAAA CCTTATCAAT GTGAAGAATG TGGCAAAATG 
TTTTACTGTT CTTCAAACCT TAAGCAACAT CAAATAACTC ATTCTCAAGA GAAACCCTAC 
AAGTGTGAAG TATGTGGCAA GGTCTTTAGA ACTTGCTGGC AACTTTCTAA ACACCTGAGA 
ATCCATTCTG GAGAGAAACC ATACAAGTGT GAGGAATGTG GCAAAGCCTT TTATACTCTC 
TCCTACCTTA CTCAGCACAA ATTAGGTCAC ACTGGGGAGA AACCTTACAA ATGTGAGGAA 
TGTGGCAAAA CCTTTTACTA CCCTTCAGTC CTTAAGGAAC ACCTAGCAAT TCATTCTGGA 
GAGAAACCGT ACAGGTGTGA TGAATGTGGC AAGGACTTTT GTACTCGCTC AGGACGTTCC 
AGACACCAGA GAATCCATAC TGGAGAGAAA CCGTACAAGT GTGAGCAATG TGGAAAGGCC 
TTCAGTACCC ATTCATACCT TTCTCATCAC AAGATAGTTC ACACTGGACA TAAACCCTAC 
AAGTGTGAAG AGTGTGGCAA AAAATTTTAT TATCCTTCTC GACTTAAGGA GCATCAAAGG 
ATTCATTCTC AAGAGAATCC CTACAAGTGT GAAATATGTG GCAAAGCCTT TTATACTCAC 
TCATACTTTA CCCAGCACAA ATTAGGTCAC ACTGGGGAGA AACCTTACAA ATGTGAGGAA 
TGTGGCAAAA CCTTTTACTA CCCTTCAATC CTTAAGGAAC ACCTAGCAAT TCATTCTGGA 
AAGAAACCGT ACAGGTGTGA AGAATGTGGC AAGGACTTTT GTACTCGCTC AGGACGTTCC 
AGACACCAAA GAATCCATAC TGGAGAGAAA CCCCACAAGT GTGAAGAATG CGGAAAAGTC 
TTTTCTACTC ATTCATATCT TACTCAGCAC AAAGTAGTCC ACTCTGGAGA GAAGCCCTAC 
AAGTGTGAAG AGTGTGGCAA AAAGTTTTAC TATCCTTCTC GCCTTAAGGA GCATCAAAGG 
ATTCATTCTC AAGAGAATCC TTACAAGTGT GAAATATGTG GCAATGTATT TTGTACTCCC 
AAAGGACTTT CGAAACACCA GAGATTTCAT ACTGGAGAGA AACCCTACAA GTGTGAAGAA 
TGCGGGAAAA TGTTTTATTA TCCTTCTCGA CTTAAGGAAC ATCAAAGAAT TCATTCTCAA 
GAGAATCCTT ACAAGTGTGA AATATGTGGC AAAGCCTTTT ATACTCACTC CTACCTTACT 
CAGCACAAAT TAGGTCATAC TGGGGAGAAA CCTTACAAAT GTGAGGAATG TGGCAAAACC 
TTTTACTACC CTTCAATCCT TAAGGAACAC CTAGCAATTC ATTCTGGAAA GAAACCGTAC 
AGGTGTGAAG AATGTGGCAA GGACTTTTGT ACTCGCTCAG GACGTTCCAG ACACCAGAGA 
ATCCATACTG GAGAGAAACC CTACAAGTGT GAGCAATGTG GAAAGGCCTT CAGTACTCAC 
TCATACCTTT CTCAACACAA GGTAGTCCAC TCTGGAGAGA AGCCCTACAA GTGTGAAGAG 
TGTGGCAAAA TGTTTTACTA TTCTTCTCGA CTTAAGGAGC ATCAAAGGAT TCATTCTCAA 
GAGAATCCCT ACAAGTGTGA AGTGTGTGGC AAAGTCTTTA GTGCTCACTT AGAACTTGCT 
ACCCACCTAA GTATTCATTC CGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Epigenetic gene silencing by the SRY protein is mediated by a KRAB-O protein that recruits the KAP1 co-repressor machinery.
The Journal of biological chemistry284(51)35670-80(2009 Dec)
Peng H,Ivanov AV,Oh HJ,Lau YF,Rauscher FJ 3rd

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

Functional analysis of the SRY-KRAB interaction in mouse sex determination.
Biology of the cell101(1)55-67(2009 Jan)
Polanco JC,Wilhelm D,Mizusaki H,Jackson A,Browne C,Davidson T,Harley V,Sinclair A,Koopman P

Sry associates with the heterochromatin protein 1 complex by interacting with a KRAB domain protein.
Biology of reproduction72(2)407-15(2005 Feb)
Oh HJ,Li Y,Lau YF

Regulator of sex-limitation (Rsl) encodes a pair of KRAB zinc-finger genes that control sexually dimorphic liver gene expression.
Genes & development17(21)2664-74(2003 Nov)
Krebs CJ,Larkins LK,Price R,Tullis KM,Miller RD,Robins DM