Supt4a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Supt4a
Entrez Gene ID	20922
Full Name	suppressor of Ty 4A
Synonyms	AL022777,Supt4h,Supt4h1,Supt4h1a
General protein information	Preferred Names suppressor of Ty 4A Names transcription elongation factor SPT4-A DRB sensitivity-inducing factor small subunit 1 DSIF small subunit 1 Transcription elongation factor SPT4-A suppressor of Ty 4 homolog 1 transcription elongation factor SPT4 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 C\|11 52.2 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006532742.2	XP_006532805.1	transcription elongation factor SPT4-A isoform X1
NM_009296.2	NP_033322.1	transcription elongation factor SPT4-A isoform 1
XM_006532741.2	XP_006532804.1	transcription elongation factor SPT4-A isoform X1
NM_009296.1	NP_033322.1	transcription elongation factor SPT4-A
NM_001355753.1	NP_001342682.1	transcription elongation factor SPT4-A isoform 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SUPT4H1	XP_523811.2
	SUPT4H1	XP_001106749.1
Drosophila melanogaster (fruit fly)	spt4	NP_610802.1
Caenorhabditis elegans (roundworm)	spt-4	NP_497135.1
Mus musculus (house mouse)	Supt4a	NP_033322.1
Danio rerio (zebrafish)	supt4h1	NP_001002509.1
Bos taurus (cattle)	SUPT4H1	NP_001029964.1
Rattus norvegicus (Norway rat)	Supt4h1	NP_001099298.2
Neurospora crassa	NCU03838	XP_960597.1
Xenopus tropicalis (tropical clawed frog)	supt4h1	NP_001016679.1
Magnaporthe oryzae (rice blast fungus)	MGG_06943	XP_003709683.1
Homo sapiens (human)	SUPT4H1	NP_003159.1
Canis lupus familiaris (dog)	SUPT4H1	XP_853156.2
Gallus gallus (chicken)	SUPT4H1	NP_001244215.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005428	XP_315434.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Effects on murine behavior and lifespan of selectively decreasing expression of mutant huntingtin allele by supt4h knockdown.
Cheng HM, Chern Y, Chen IH, Liu CR, Li SH, Chun SJ, Rigo F, Bennett CF, Deng N, Feng Y, Lin CS, Yan YT, Cohen SN, Cheng TH
PLoS genetics11(3)e1005043(2015 Mar)

Spt4 is selectively required for transcription of extended trinucleotide repeats.
Liu CR, Chang CR, Chern Y, Wang TH, Hsieh WC, Shen WC, Chang CY, Chu IC, Deng N, Cohen SN, Cheng TH
Cell148(4)690-701(2012 Feb)

The DSIF subunits Spt4 and Spt5 have distinct roles at various phases of immunoglobulin class switch recombination.
Stanlie A, Begum NA, Akiyama H, Honjo T
PLoS genetics8(4)e1002675(2012)

Antisense RNA polymerase II divergent transcripts are P-TEFb dependent and substrates for the RNA exosome.
Flynn RA, Almada AE, Zamudio JR, Sharp PA
Proceedings of the National Academy of Sciences of the United States of America108(26)10460-5(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

reduction of SUPT4H in mouse brains is associated with decreased HTT protein aggregation, and in R6/2 mice, also with prolonged lifespan and delay of the motor impairment that normally develops in these animals.
Title: Effects on murine behavior and lifespan of selectively decreasing expression of mutant huntingtin allele by supt4h knockdown.

These findings indicate that Supt4h is differentially required for expression of the mutant Htt allele in mouse striatal neurons.
Title: Spt4 is selectively required for transcription of extended trinucleotide repeats.

our findings indicate that Spt4 and Spt5 have essential functions in the DNA repair phase of immunoglobulin class switch recombination.
Title: The DSIF subunits Spt4 and Spt5 have distinct roles at various phases of immunoglobulin class switch recombination.

The following Supt4a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Supt4a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu45931	NM_001355753.1 Latest version!	Mus musculus SPT4A, DSIF elongation factor subunit (Supt4a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu45931	XM_006532742.2 Latest version!	Mus musculus suppressor of Ty 4A (Supt4a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu45931	XM_006532741.2 Latest version!	Mus musculus suppressor of Ty 4A (Supt4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu03890	NM_009296.2 Latest version!	Mus musculus SPT4A, DSIF elongation factor subunit (Supt4a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
View Old Accession Versions >>
OMu03890	NM_009296.1	Mus musculus suppressor of Ty 4A (Supt4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Supt4a ( NM_001355753.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001342682.1 Mus musculus SPT4A, DSIF elongation factor subunit (Supt4a), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu45931
Clone ID Related Accession (Same CDS sequence)	XM_006532742.2 , XM_006532741.2 , NM_001355753.1
Accession Version	NM_001355753.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-24
Organism	Mus musculus(house mouse)
Product	transcription elongation factor SPT4-A isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL604022.21, W47755.1, AK002990.1 and BQ176059.1. On Sep 27, 2017 this sequence version replaced XM_006532742.2. ##Evidence-Data-START## Transcript exon combination :: AA087130.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGAAGC TGGTTGTGTT GATGCAGCCT TCAGTCCTAG CTCTCAAGCT GAGCAGGGGA 
CACAAGTGTC GGGTAGCCTT CTGCTGCATC ATGAGACGAC GCCCCCATCT AAAAATTAAA 
ATAATCATTG CGATGATGAG TCCAGAGGAC AGCTGGGTCT CCAAGTGGCA GCGAGTCAGT 
AACTTTAAGC CAGGTGTATA TGCTGTGTCC GTCACTGGTC GCCTGCCCCA AGGAATCGTG 
CGGGAGCTGA AAAGTCGAGG AGTGGCCTAC AAATCCAGAG ACACAGCAAT AAAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342682.1
CDS	171..470
Translation	MTKLVVLMQPSVLALKLSRGHKCRVAFCCIMRRRPHLKIKIIIAMMSPEDSWVSKWQRVSNFKPGVYAVSVTGRLPQGIVRELKSRGVAYKSRDTAIKT

Target ORF information:

RefSeq Version	NM_001355753.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPT4A, DSIF elongation factor subunit (Supt4a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355753.1

ORF Insert Sequence:

ATGACGAAGC TGGTTGTGTT GATGCAGCCT TCAGTCCTAG CTCTCAAGCT GAGCAGGGGA 
CACAAGTGTC GGGTAGCCTT CTGCTGCATC ATGAGACGAC GCCCCCATCT AAAAATTAAA 
ATAATCATTG CGATGATGAG TCCAGAGGAC AGCTGGGTCT CCAAGTGGCA GCGAGTCAGT 
AACTTTAAGC CAGGTGTATA TGCTGTGTCC GTCACTGGTC GCCTGCCCCA AGGAATCGTG 
CGGGAGCTGA AAAGTCGAGG AGTGGCCTAC AAATCCAGAG ACACAGCAAT AAAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Supt4a ( XM_006532742.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532805.1 Mus musculus suppressor of Ty 4A (Supt4a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu45931
Clone ID Related Accession (Same CDS sequence)	XM_006532742.2 , XM_006532741.2 , NM_001355753.1
Accession Version	XM_006532742.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	transcription elongation factor SPT4-A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532742.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGAAGC TGGTTGTGTT GATGCAGCCT TCAGTCCTAG CTCTCAAGCT GAGCAGGGGA 
CACAAGTGTC GGGTAGCCTT CTGCTGCATC ATGAGACGAC GCCCCCATCT AAAAATTAAA 
ATAATCATTG CGATGATGAG TCCAGAGGAC AGCTGGGTCT CCAAGTGGCA GCGAGTCAGT 
AACTTTAAGC CAGGTGTATA TGCTGTGTCC GTCACTGGTC GCCTGCCCCA AGGAATCGTG 
CGGGAGCTGA AAAGTCGAGG AGTGGCCTAC AAATCCAGAG ACACAGCAAT AAAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532805.1
CDS	215..514
Translation	MTKLVVLMQPSVLALKLSRGHKCRVAFCCIMRRRPHLKIKIIIAMMSPEDSWVSKWQRVSNFKPGVYAVSVTGRLPQGIVRELKSRGVAYKSRDTAIKT

Target ORF information:

RefSeq Version	XM_006532742.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus suppressor of Ty 4A (Supt4a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532742.2

ORF Insert Sequence:

ATGACGAAGC TGGTTGTGTT GATGCAGCCT TCAGTCCTAG CTCTCAAGCT GAGCAGGGGA 
CACAAGTGTC GGGTAGCCTT CTGCTGCATC ATGAGACGAC GCCCCCATCT AAAAATTAAA 
ATAATCATTG CGATGATGAG TCCAGAGGAC AGCTGGGTCT CCAAGTGGCA GCGAGTCAGT 
AACTTTAAGC CAGGTGTATA TGCTGTGTCC GTCACTGGTC GCCTGCCCCA AGGAATCGTG 
CGGGAGCTGA AAAGTCGAGG AGTGGCCTAC AAATCCAGAG ACACAGCAAT AAAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Supt4a ( XM_006532741.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532804.1 Mus musculus suppressor of Ty 4A (Supt4a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu45931
Clone ID Related Accession (Same CDS sequence)	XM_006532742.2 , XM_006532741.2 , NM_001355753.1
Accession Version	XM_006532741.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	transcription elongation factor SPT4-A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532741.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGAAGC TGGTTGTGTT GATGCAGCCT TCAGTCCTAG CTCTCAAGCT GAGCAGGGGA 
CACAAGTGTC GGGTAGCCTT CTGCTGCATC ATGAGACGAC GCCCCCATCT AAAAATTAAA 
ATAATCATTG CGATGATGAG TCCAGAGGAC AGCTGGGTCT CCAAGTGGCA GCGAGTCAGT 
AACTTTAAGC CAGGTGTATA TGCTGTGTCC GTCACTGGTC GCCTGCCCCA AGGAATCGTG 
CGGGAGCTGA AAAGTCGAGG AGTGGCCTAC AAATCCAGAG ACACAGCAAT AAAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532804.1
CDS	246..545
Translation	MTKLVVLMQPSVLALKLSRGHKCRVAFCCIMRRRPHLKIKIIIAMMSPEDSWVSKWQRVSNFKPGVYAVSVTGRLPQGIVRELKSRGVAYKSRDTAIKT

Target ORF information:

RefSeq Version	XM_006532741.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus suppressor of Ty 4A (Supt4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532741.2

ORF Insert Sequence:

ATGACGAAGC TGGTTGTGTT GATGCAGCCT TCAGTCCTAG CTCTCAAGCT GAGCAGGGGA 
CACAAGTGTC GGGTAGCCTT CTGCTGCATC ATGAGACGAC GCCCCCATCT AAAAATTAAA 
ATAATCATTG CGATGATGAG TCCAGAGGAC AGCTGGGTCT CCAAGTGGCA GCGAGTCAGT 
AACTTTAAGC CAGGTGTATA TGCTGTGTCC GTCACTGGTC GCCTGCCCCA AGGAATCGTG 
CGGGAGCTGA AAAGTCGAGG AGTGGCCTAC AAATCCAGAG ACACAGCAAT AAAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Supt4a ( NM_009296.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033322.1 Mus musculus SPT4A, DSIF elongation factor subunit (Supt4a), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu03890
Clone ID Related Accession (Same CDS sequence)	NM_009296.2 , NM_009296.1
Accession Version	NM_009296.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-24
Organism	Mus musculus(house mouse)
Product	transcription elongation factor SPT4-A isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DV061290.1, DV044501.1 and BQ176059.1. On Sep 27, 2017 this sequence version replaced NM_009296.1. ##Evidence-Data-START## Transcript exon combination :: BQ176059.1, DV061290.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCTGG AGACGGTACC AAAGGACCTG CGGCATCTGC GGGCTTGTTT GCTGTGCTCG 
TTAGTCAAGA CTATAGACCA GTTCGAATAT GATGGGTGTG ACAATTGCGA TGCATACCTA 
CAAATGAAGG GCAACAGAGA GATGGTTTAT GACTGCACCA GCTCTTCATT TGATGGAATC 
ATTGCGATGA TGAGTCCAGA GGACAGCTGG GTCTCCAAGT GGCAGCGAGT CAGTAACTTT 
AAGCCAGGTG TATATGCTGT GTCCGTCACT GGTCGCCTGC CCCAAGGAAT CGTGCGGGAG 
CTGAAAAGTC GAGGAGTGGC CTACAAATCC AGAGACACAG CAATAAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033322.1
CDS	50..403
Translation	MALETVPKDLRHLRACLLCSLVKTIDQFEYDGCDNCDAYLQMKGNREMVYDCTSSSFDGIIAMMSPEDSWVSKWQRVSNFKPGVYAVSVTGRLPQGIVRELKSRGVAYKSRDTAIKT

Target ORF information:

RefSeq Version	NM_009296.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SPT4A, DSIF elongation factor subunit (Supt4a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009296.2

ORF Insert Sequence:

ATGGCCCTGG AGACGGTACC AAAGGACCTG CGGCATCTGC GGGCTTGTTT GCTGTGCTCG 
TTAGTCAAGA CTATAGACCA GTTCGAATAT GATGGGTGTG ACAATTGCGA TGCATACCTA 
CAAATGAAGG GCAACAGAGA GATGGTTTAT GACTGCACCA GCTCTTCATT TGATGGAATC 
ATTGCGATGA TGAGTCCAGA GGACAGCTGG GTCTCCAAGT GGCAGCGAGT CAGTAACTTT 
AAGCCAGGTG TATATGCTGT GTCCGTCACT GGTCGCCTGC CCCAAGGAAT CGTGCGGGAG 
CTGAAAAGTC GAGGAGTGGC CTACAAATCC AGAGACACAG CAATAAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Supt4a ( NM_009296.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033322.1 Mus musculus suppressor of Ty 4A (Supt4a), mRNA.

Price & Availability↑

CloneID	OMu03890
Clone ID Related Accession (Same CDS sequence)	NM_009296.2 , NM_009296.1
Accession Version	NM_009296.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-24
Organism	Mus musculus(House mouse)
Product	transcription elongation factor SPT4-A
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U96809.1. ##Evidence-Data-START## Transcript exon combination :: BC061174.1, CA495186.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCCCTGG AGACGGTACC AAAGGACCTG CGGCATCTGC GGGCTTGTTT GCTGTGCTCG 
TTAGTCAAGA CTATAGACCA GTTCGAATAT GATGGGTGTG ACAATTGCGA TGCATACCTA 
CAAATGAAGG GCAACAGAGA GATGGTTTAT GACTGCACCA GCTCTTCATT TGATGGAATC 
ATTGCGATGA TGAGTCCAGA GGACAGCTGG GTCTCCAAGT GGCAGCGAGT CAGTAACTTT 
AAGCCAGGTG TATATGCTGT GTCCGTCACT GGTCGCCTGC CCCAAGGAAT CGTGCGGGAG 
CTGAAAAGTC GAGGAGTGGC CTACAAATCC AGAGACACAG CAATAAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033322.1
CDS	36..389
Translation	MALETVPKDLRHLRACLLCSLVKTIDQFEYDGCDNCDAYLQMKGNREMVYDCTSSSFDGIIAMMSPEDSWVSKWQRVSNFKPGVYAVSVTGRLPQGIVRELKSRGVAYKSRDTAIKT

Target ORF information:

RefSeq Version	NM_009296.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus suppressor of Ty 4A (Supt4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009296.1

ORF Insert Sequence:

ATGGCCCTGG AGACGGTACC AAAGGACCTG CGGCATCTGC GGGCTTGTTT GCTGTGCTCG 
TTAGTCAAGA CTATAGACCA GTTCGAATAT GATGGGTGTG ACAATTGCGA TGCATACCTA 
CAAATGAAGG GCAACAGAGA GATGGTTTAT GACTGCACCA GCTCTTCATT TGATGGAATC 
ATTGCGATGA TGAGTCCAGA GGACAGCTGG GTCTCCAAGT GGCAGCGAGT CAGTAACTTT 
AAGCCAGGTG TATATGCTGT GTCCGTCACT GGTCGCCTGC CCCAAGGAAT CGTGCGGGAG 
CTGAAAAGTC GAGGAGTGGC CTACAAATCC AGAGACACAG CAATAAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Effects on murine behavior and lifespan of selectively decreasing expression of mutant huntingtin allele by supt4h knockdown.
PLoS genetics11(3)e1005043(2015 Mar)
Cheng HM,Chern Y,Chen IH,Liu CR,Li SH,Chun SJ,Rigo F,Bennett CF,Deng N,Feng Y,Lin CS,Yan YT,Cohen SN,Cheng TH

Spt4 is selectively required for transcription of extended trinucleotide repeats.
Cell148(4)690-701(2012 Feb)
Liu CR,Chang CR,Chern Y,Wang TH,Hsieh WC,Shen WC,Chang CY,Chu IC,Deng N,Cohen SN,Cheng TH

The DSIF subunits Spt4 and Spt5 have distinct roles at various phases of immunoglobulin class switch recombination.
PLoS genetics8(4)e1002675(2012)
Stanlie A,Begum NA,Akiyama H,Honjo T

Antisense RNA polymerase II divergent transcripts are P-TEFb dependent and substrates for the RNA exosome.
Proceedings of the National Academy of Sciences of the United States of America108(26)10460-5(2011 Jun)
Flynn RA,Almada AE,Zamudio JR,Sharp PA

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Supt4a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム