Ptk6 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ptk6
Entrez Gene ID	20459
Full Name	PTK6 protein tyrosine kinase 6
Synonyms	BRK,Sik,Tksk,tks
General protein information	Preferred Names PTK6 protein tyrosine kinase 6 Names protein-tyrosine kinase 6 src-related intestinal kinase tyrosine kinase identified from skin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 H4\|2 103.62 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001356304.1	NP_001343233.1	protein-tyrosine kinase 6 isoform 2
NM_009184.2	NP_033210.1	protein-tyrosine kinase 6 isoform 1
XM_017316742.1	XP_017172231.1	protein-tyrosine kinase 6 isoform X2
XM_006500585.3	XP_006500648.1	protein-tyrosine kinase 6 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PTK6	XP_514781.3
Mus musculus (house mouse)	Ptk6	NP_033210.1
Bos taurus (cattle)	PTK6	NP_001179611.1
Danio rerio (zebrafish)	ptk6b	NP_001070140.1
Danio rerio (zebrafish)	ptk6a	XP_682778.4
Xenopus tropicalis (tropical clawed frog)	ptk6	XP_002942429.2
Homo sapiens (human)	PTK6	NP_005966.1
Rattus norvegicus (Norway rat)	Ptk6	NP_001102438.1

Gene Ontology
Bibliography

Related articles in PubMed

TNF?/IFN? Mediated Intestinal Epithelial Barrier Dysfunction Is Attenuated by MicroRNA-93 Downregulation of PTK6 in Mouse Colonic Epithelial Cells.
Haines RJ, Beard RS Jr, Eitner RA, Chen L, Wu MH
PloS one11(4)e0154351(2016)

Protein Tyrosine Kinase 6 Regulates UVB-Induced Signaling and Tumorigenesis in Mouse Skin.
Chastkofsky MI, Bie W, Ball-Kell SM, He YY, Tyner AL
The Journal of investigative dermatology135(10)2492-2501(2015 Oct)

Protein tyrosine kinase 6 promotes ERBB2-induced mammary gland tumorigenesis in the mouse.
Peng M, Ball-Kell SM, Tyner AL
Cell death & disease6e1848(2015 Aug)

PTK6 Potentiates Gemcitabine-Induced Apoptosis by Prolonging S-phase and Enhancing DNA Damage in Pancreatic Cancer.
Ono H, Basson MD, Ito H
Molecular cancer research : MCR13(8)1174-84(2015 Aug)

Protein tyrosine kinase 6 mediates TNF?-induced endothelial barrier dysfunction.
Haines RJ, Beard RS Jr, Wu MH
Biochemical and biophysical research communications456(1)190-6(2015 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PTK6 may act as a novel mediator of TNFalpha/IFNgamma intestinal epithelial permeability during inflammatory injury, and miR-93 may protect intestinal epithelial barrier function, at least in part, by targeting PTK6
Title: TNF?/IFN? Mediated Intestinal Epithelial Barrier Dysfunction Is Attenuated by MicroRNA-93 Downregulation of PTK6 in Mouse Colonic Epithelial Cells.

PTK; expression increases in epidermis following UVB. indicates tumorigenesis in mouse skin.
Title: Protein Tyrosine Kinase 6 Regulates UVB-Induced Signaling and Tumorigenesis in Mouse Skin.

Ptk6 promotes ERBB2-induced mammary gland tumorigenesis in the mouse.
Title: Protein tyrosine kinase 6 promotes ERBB2-induced mammary gland tumorigenesis in the mouse.

PTK6 prolongs S-phase and increases the ability of gemcitabine to cause DNA damage in vitro and in vivo
Title: PTK6 Potentiates Gemcitabine-Induced Apoptosis by Prolonging S-phase and Enhancing DNA Damage in Pancreatic Cancer.

Protein tyrosine kinase 6 has a role in mediating TNFalpha-induced endothelial barrier dysfunction
Title: Protein tyrosine kinase 6 mediates TNF?-induced endothelial barrier dysfunction.

The following Ptk6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ptk6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu14089	NM_009184.2 Latest version!	Mus musculus PTK6 protein tyrosine kinase 6 (Ptk6), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OMu31149	XM_006500585.3 Latest version!	Mus musculus PTK6 protein tyrosine kinase 6 (Ptk6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu80162	NM_001356304.1 Latest version!	Mus musculus PTK6 protein tyrosine kinase 6 (Ptk6), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu80162	XM_017316742.1 Latest version!	Mus musculus PTK6 protein tyrosine kinase 6 (Ptk6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ptk6 ( NM_009184.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033210.1 Mus musculus PTK6 protein tyrosine kinase 6 (Ptk6), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu14089
Clone ID Related Accession (Same CDS sequence)	NM_009184.2
Accession Version	NM_009184.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	protein-tyrosine kinase 6 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL450341.10. On Mar 21, 2007 this sequence version replaced NM_009184.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U16805.1, BC125342.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGTCTT GGGACAAGGC TCACCTGGGT CCTAAGTATG TGGGCCTCTG GGACTTCAAG 
GCACGGACAG ATGAGGAGCT GAGCTTTCAG GCAGGAGACC TCCTCCATGT TACCAAGAAG 
GAGGAACTGT GGTGGTGGGC CACCCTGCTG GATGCAGAAG GCAAGGCCTT GGCTGAGGGC 
TATGTGCCTC ACAACTACCT GGCTGAGAAG GAAACTGTGG AGTCTGAACC GTGGTTCTTT 
GGTTGCATCT CCCGCTCAGA GGCCATGCAC AGGCTGCAGG CTGAGGACAA CTCGAAGGGT 
GCCTTCCTGA TCAGAGTCAG CCAGAAGCCA GGAGCAGACT ATGTCCTCTC TGTCCGGGAT 
GCTCAGGCCG TGCGACATTA CAGGATCTGG AAGAACAACG AGGGCCGGCT GCACCTGAAT 
GAGGCGGTAT CCTTCTCCAA TCTGTCTGAG CTTGTGGACT ACCATAAGAC CCAGAGCCTG 
TCTCATGGCC TACAGCTGTC CATGCCCTGC TGGAAGCACA AAACTGAGCC CTTGCCCCAC 
TGGGATGACT GGGAGAGGCC GAGGGAGGAG TTCACACTCT GTAAGAAGCT GGGGGCCGGC 
TACTTTGGGG AGGTCTTTGA AGCGCTCTGG AAAGGCCAGG TCCATGTGGC TGTGAAGGTG 
ATCTCTAGAG ACAATCTCCT GCACCAGCAC ACCTTCCAGG CTGAGATTCA GGCCATGAAG 
AAGCTGCGGC ACAAGCACAT CCTGTCACTG TACGCTGTGG CGACTGCAGG GGACCCGGTC 
TACATCATCA CGGAGCTCAT GCCCAAGGGG AACCTGCTGC AGCTACTGCG TGACTCTGAT 
GAGAAAGCCC TGCCTATTTT GGAGCTGGTG GACTTTGCAT CACAGGTTGC TGAGGGCATG 
TGCTACCTGG AATCTCAGAA TTACATCCAC CGTGACCTGG CTGCAAGGAA CGTTCTTGTT 
ACAGAGAACA ATCTCTGCAA AGTGGGGGAC TTTGGGCTTG CCAGGCTTGT CAAGGAGGAC 
ATCTACCTTT CCCATGAGCA CAATGTCCCC TACAAATGGA CAGCACCTGA GGCACTTTCC 
CGAGGGCATT ACTCCATCAA GTCTGATGTC TGGTCTTTTG GAGTTCTTCT TCATGAAATT 
TTCAGCAGGG GGCAGATGCC CTACCCAGGC ATGTCCAATC ATGAAACCTT CCTGAGGGTG 
GATGCCGGCT ACCGCATGCC CTGCCCTCTG GAGTGCCCAC CCAACATACA CAAGCTGATG 
CTCAGCTGCT GGAGCAGAGA CCCCAAGCAG AGACCTTGCT TCAAAGACCT GTGTGAGAAA 
CTCACAGGTA TCACCAGGTA TGAGAACCTG GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033210.1
CDS	286..1641
Translation	MVSWDKAHLGPKYVGLWDFKARTDEELSFQAGDLLHVTKKEELWWWATLLDAEGKALAEGYVPHNYLAEKETVESEPWFFGCISRSEAMHRLQAEDNSKGAFLIRVSQKPGADYVLSVRDAQAVRHYRIWKNNEGRLHLNEAVSFSNLSELVDYHKTQSLSHGLQLSMPCWKHKTEPLPHWDDWERPREEFTLCKKLGAGYFGEVFEALWKGQVHVAVKVISRDNLLHQHTFQAEIQAMKKLRHKHILSLYAVATAGDPVYIITELMPKGNLLQLLRDSDEKALPILELVDFASQVAEGMCYLESQNYIHRDLAARNVLVTENNLCKVGDFGLARLVKEDIYLSHEHNVPYKWTAPEALSRGHYSIKSDVWSFGVLLHEIFSRGQMPYPGMSNHETFLRVDAGYRMPCPLECPPNIHKLMLSCWSRDPKQRPCFKDLCEKLTGITRYENLV

Target ORF information:

RefSeq Version	NM_009184.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus PTK6 protein tyrosine kinase 6 (Ptk6), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009184.2

ORF Insert Sequence:

ATGGTGTCTT GGGACAAGGC TCACCTGGGT CCTAAGTATG TGGGCCTCTG GGACTTCAAG 
GCACGGACAG ATGAGGAGCT GAGCTTTCAG GCAGGAGACC TCCTCCATGT TACCAAGAAG 
GAGGAACTGT GGTGGTGGGC CACCCTGCTG GATGCAGAAG GCAAGGCCTT GGCTGAGGGC 
TATGTGCCTC ACAACTACCT GGCTGAGAAG GAAACTGTGG AGTCTGAACC GTGGTTCTTT 
GGTTGCATCT CCCGCTCAGA GGCCATGCAC AGGCTGCAGG CTGAGGACAA CTCGAAGGGT 
GCCTTCCTGA TCAGAGTCAG CCAGAAGCCA GGAGCAGACT ATGTCCTCTC TGTCCGGGAT 
GCTCAGGCCG TGCGACATTA CAGGATCTGG AAGAACAACG AGGGCCGGCT GCACCTGAAT 
GAGGCGGTAT CCTTCTCCAA TCTGTCTGAG CTTGTGGACT ACCATAAGAC CCAGAGCCTG 
TCTCATGGCC TACAGCTGTC CATGCCCTGC TGGAAGCACA AAACTGAGCC CTTGCCCCAC 
TGGGATGACT GGGAGAGGCC GAGGGAGGAG TTCACACTCT GTAAGAAGCT GGGGGCCGGC 
TACTTTGGGG AGGTCTTTGA AGCGCTCTGG AAAGGCCAGG TCCATGTGGC TGTGAAGGTG 
ATCTCTAGAG ACAATCTCCT GCACCAGCAC ACCTTCCAGG CTGAGATTCA GGCCATGAAG 
AAGCTGCGGC ACAAGCACAT CCTGTCACTG TACGCTGTGG CGACTGCAGG GGACCCGGTC 
TACATCATCA CGGAGCTCAT GCCCAAGGGG AACCTGCTGC AGCTACTGCG TGACTCTGAT 
GAGAAAGCCC TGCCTATTTT GGAGCTGGTG GACTTTGCAT CACAGGTTGC TGAGGGCATG 
TGCTACCTGG AATCTCAGAA TTACATCCAC CGTGACCTGG CTGCAAGGAA CGTTCTTGTT 
ACAGAGAACA ATCTCTGCAA AGTGGGGGAC TTTGGGCTTG CCAGGCTTGT CAAGGAGGAC 
ATCTACCTTT CCCATGAGCA CAATGTCCCC TACAAATGGA CAGCACCTGA GGCACTTTCC 
CGAGGGCATT ACTCCATCAA GTCTGATGTC TGGTCTTTTG GAGTTCTTCT TCATGAAATT 
TTCAGCAGGG GGCAGATGCC CTACCCAGGC ATGTCCAATC ATGAAACCTT CCTGAGGGTG 
GATGCCGGCT ACCGCATGCC CTGCCCTCTG GAGTGCCCAC CCAACATACA CAAGCTGATG 
CTCAGCTGCT GGAGCAGAGA CCCCAAGCAG AGACCTTGCT TCAAAGACCT GTGTGAGAAA 
CTCACAGGTA TCACCAGGTA TGAGAACCTG GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptk6 ( XM_006500585.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500648.1 Mus musculus PTK6 protein tyrosine kinase 6 (Ptk6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu31149
Clone ID Related Accession (Same CDS sequence)	XM_006500585.3
Accession Version	XM_006500585.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein-tyrosine kinase 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006500585.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTCTT GGGACAAGGC TCACCTGGGT CCTAAGTATG TGGGCCTCTG GGACTTCAAG 
GCACGGACAG ATGAGGAGCT GAGCTTTCAG GCAGGAGACC TCCTCCATGT TACCAAGAAG 
GAGGAACTGT GGTGGTGGGC CACCCTGCTG GATGCAGAAG GCAAGGCCTT GGCTGAGGGC 
TATGTGCCTC ACAACTACCT GGCTGAGAAG GAAACTGTGG AGTCTGAACC GTGGTTCTTT 
GGTTGCATCT CCCGCTCAGA GGCCATGCAC AGGCTGCAGG CTGAGGACAA CTCGAAGGGT 
GCCTTCCTGA TCAGAGTCAG CCAGAAGCCA GGAGCAGACT ATGTCCTCTC TGTCCGGGAT 
GCTCAGGCCG TGCGACATTA CAGGATCTGG AAGAACAACG AGGGCCGGCT GCACCTGAAT 
GAGGCGGTAT CCTTCTCCAA TCTGTCTGAG CTTGTGGACT ACCATAAGAC CCAGAGCCTG 
TCTCATGGCC TACAGCTGTC CATGCCCTGC TGGAAGCACA AAACTGAGCC CTTGCCCCAC 
TGGGATGACT GGGAGAGGCC GAGGGAGGAG TTCACACTCT GTAAGAAGCT GGGGGCCGGC 
TACTTTGGGG AGGTCTTTGA AGCGCTCTGG AAAGGCCAGG TCCATGTGGC TGTGAAGGTG 
ATCTCTAGAG ACAATCTCCT GCACCAGCAC ACCTTCCAGG CTGAGATTCA GGCCATGAAG 
AAGCTGCGGC ACAAGCACAT CCTGTCACTG TACGCTGTGG CGACTGCAGG GGACCCGGTC 
TACATCATCA CGGAGCTCAT GCCCAAGGGG AACCTGCTGC AGCTACTGCG TGACTCTGAT 
GAGAAAGCCC TGCCTATTTT GGAGCTGGTG GACTTTGCAT CACAGGTTGC TGAGGGCATG 
TGCTACCTGG AATCTCAGAA TTACATCCAC CGTGACCTGG CTGCAAGGAA CGTTCTTGTT 
ACAGAGAACA ATCTCTGCAA AGTGGGGGAC TTTGGGCTTG CCAGGCTTGT CAAGGCATGT 
CCAATCATGA AACCTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500648.1
CDS	149..1189
Translation	MVSWDKAHLGPKYVGLWDFKARTDEELSFQAGDLLHVTKKEELWWWATLLDAEGKALAEGYVPHNYLAEKETVESEPWFFGCISRSEAMHRLQAEDNSKGAFLIRVSQKPGADYVLSVRDAQAVRHYRIWKNNEGRLHLNEAVSFSNLSELVDYHKTQSLSHGLQLSMPCWKHKTEPLPHWDDWERPREEFTLCKKLGAGYFGEVFEALWKGQVHVAVKVISRDNLLHQHTFQAEIQAMKKLRHKHILSLYAVATAGDPVYIITELMPKGNLLQLLRDSDEKALPILELVDFASQVAEGMCYLESQNYIHRDLAARNVLVTENNLCKVGDFGLARLVKACPIMKPS

Target ORF information:

RefSeq Version	XM_006500585.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus PTK6 protein tyrosine kinase 6 (Ptk6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500585.3

ORF Insert Sequence:

ATGGTGTCTT GGGACAAGGC TCACCTGGGT CCTAAGTATG TGGGCCTCTG GGACTTCAAG 
GCACGGACAG ATGAGGAGCT GAGCTTTCAG GCAGGAGACC TCCTCCATGT TACCAAGAAG 
GAGGAACTGT GGTGGTGGGC CACCCTGCTG GATGCAGAAG GCAAGGCCTT GGCTGAGGGC 
TATGTGCCTC ACAACTACCT GGCTGAGAAG GAAACTGTGG AGTCTGAACC GTGGTTCTTT 
GGTTGCATCT CCCGCTCAGA GGCCATGCAC AGGCTGCAGG CTGAGGACAA CTCGAAGGGT 
GCCTTCCTGA TCAGAGTCAG CCAGAAGCCA GGAGCAGACT ATGTCCTCTC TGTCCGGGAT 
GCTCAGGCCG TGCGACATTA CAGGATCTGG AAGAACAACG AGGGCCGGCT GCACCTGAAT 
GAGGCGGTAT CCTTCTCCAA TCTGTCTGAG CTTGTGGACT ACCATAAGAC CCAGAGCCTG 
TCTCATGGCC TACAGCTGTC CATGCCCTGC TGGAAGCACA AAACTGAGCC CTTGCCCCAC 
TGGGATGACT GGGAGAGGCC GAGGGAGGAG TTCACACTCT GTAAGAAGCT GGGGGCCGGC 
TACTTTGGGG AGGTCTTTGA AGCGCTCTGG AAAGGCCAGG TCCATGTGGC TGTGAAGGTG 
ATCTCTAGAG ACAATCTCCT GCACCAGCAC ACCTTCCAGG CTGAGATTCA GGCCATGAAG 
AAGCTGCGGC ACAAGCACAT CCTGTCACTG TACGCTGTGG CGACTGCAGG GGACCCGGTC 
TACATCATCA CGGAGCTCAT GCCCAAGGGG AACCTGCTGC AGCTACTGCG TGACTCTGAT 
GAGAAAGCCC TGCCTATTTT GGAGCTGGTG GACTTTGCAT CACAGGTTGC TGAGGGCATG 
TGCTACCTGG AATCTCAGAA TTACATCCAC CGTGACCTGG CTGCAAGGAA CGTTCTTGTT 
ACAGAGAACA ATCTCTGCAA AGTGGGGGAC TTTGGGCTTG CCAGGCTTGT CAAGGCATGT 
CCAATCATGA AACCTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptk6 ( NM_001356304.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001343233.1 Mus musculus PTK6 protein tyrosine kinase 6 (Ptk6), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu80162
Clone ID Related Accession (Same CDS sequence)	NM_001356304.1 , XM_017316742.1
Accession Version	NM_001356304.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	protein-tyrosine kinase 6 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL450341.10. On Sep 28, 2017 this sequence version replaced XM_017316742.1. Transcript Variant: This variant (2) uses an alternate splice junction compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC145042.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTGCT GGAAGCACAA AACTGAGCCC TTGCCCCACT GGGATGACTG GGAGAGGCCG 
AGGGAGGAGT TCACACTCTG TAAGAAGCTG GGGGCCGGCT ACTTTGGGGA GGTCTTTGAA 
GCGCTCTGGA AAGGCCAGGT CCATGTGGCT GTGAAGGTGA TCTCTAGAGA CAATCTCCTG 
CACCAGCACA CCTTCCAGGC TGAGATTCAG GCCATGAAGA AGCTGCGGCA CAAGCACATC 
CTGTCACTGT ACGCTGTGGC GACTGCAGGG GACCCGGTCT ACATCATCAC GGAGCTCATG 
CCCAAGGGGA ACCTGCTGCA GCTACTGCGT GACTCTGATG AGAAAGCCCT GCCTATTTTG 
GAGCTGGTGG ACTTTGCATC ACAGGTTGCT GAGGGCATGT GCTACCTGGA ATCTCAGAAT 
TACATCCACC GTGACCTGGC TGCAAGGAAC GTTCTTGTTA CAGAGAACAA TCTCTGCAAA 
GTGGGGGACT TTGGGCTTGC CAGGCTTGTC AAGGAGGACA TCTACCTTTC CCATGAGCAC 
AATGTCCCCT ACAAATGGAC AGCACCTGAG GCACTTTCCC GAGGGCATTA CTCCATCAAG 
TCTGATGTCT GGTCTTTTGG AGTTCTTCTT CATGAAATTT TCAGCAGGGG GCAGATGCCC 
TACCCAGGCA TGTCCAATCA TGAAACCTTC CTGAGGGTGG ATGCCGGCTA CCGCATGCCC 
TGCCCTCTGG AGTGCCCACC CAACATACAC AAGCTGATGC TCAGCTGCTG GAGCAGAGAC 
CCCAAGCAGA GACCTTGCTT CAAAGACCTG TGTGAGAAAC TCACAGGTAT CACCAGGTAT 
GAGAACCTGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001343233.1
CDS	756..1610
Translation	MPCWKHKTEPLPHWDDWERPREEFTLCKKLGAGYFGEVFEALWKGQVHVAVKVISRDNLLHQHTFQAEIQAMKKLRHKHILSLYAVATAGDPVYIITELMPKGNLLQLLRDSDEKALPILELVDFASQVAEGMCYLESQNYIHRDLAARNVLVTENNLCKVGDFGLARLVKEDIYLSHEHNVPYKWTAPEALSRGHYSIKSDVWSFGVLLHEIFSRGQMPYPGMSNHETFLRVDAGYRMPCPLECPPNIHKLMLSCWSRDPKQRPCFKDLCEKLTGITRYENLV

Target ORF information:

RefSeq Version	NM_001356304.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus PTK6 protein tyrosine kinase 6 (Ptk6), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356304.1

ORF Insert Sequence:

ATGCCCTGCT GGAAGCACAA AACTGAGCCC TTGCCCCACT GGGATGACTG GGAGAGGCCG 
AGGGAGGAGT TCACACTCTG TAAGAAGCTG GGGGCCGGCT ACTTTGGGGA GGTCTTTGAA 
GCGCTCTGGA AAGGCCAGGT CCATGTGGCT GTGAAGGTGA TCTCTAGAGA CAATCTCCTG 
CACCAGCACA CCTTCCAGGC TGAGATTCAG GCCATGAAGA AGCTGCGGCA CAAGCACATC 
CTGTCACTGT ACGCTGTGGC GACTGCAGGG GACCCGGTCT ACATCATCAC GGAGCTCATG 
CCCAAGGGGA ACCTGCTGCA GCTACTGCGT GACTCTGATG AGAAAGCCCT GCCTATTTTG 
GAGCTGGTGG ACTTTGCATC ACAGGTTGCT GAGGGCATGT GCTACCTGGA ATCTCAGAAT 
TACATCCACC GTGACCTGGC TGCAAGGAAC GTTCTTGTTA CAGAGAACAA TCTCTGCAAA 
GTGGGGGACT TTGGGCTTGC CAGGCTTGTC AAGGAGGACA TCTACCTTTC CCATGAGCAC 
AATGTCCCCT ACAAATGGAC AGCACCTGAG GCACTTTCCC GAGGGCATTA CTCCATCAAG 
TCTGATGTCT GGTCTTTTGG AGTTCTTCTT CATGAAATTT TCAGCAGGGG GCAGATGCCC 
TACCCAGGCA TGTCCAATCA TGAAACCTTC CTGAGGGTGG ATGCCGGCTA CCGCATGCCC 
TGCCCTCTGG AGTGCCCACC CAACATACAC AAGCTGATGC TCAGCTGCTG GAGCAGAGAC 
CCCAAGCAGA GACCTTGCTT CAAAGACCTG TGTGAGAAAC TCACAGGTAT CACCAGGTAT 
GAGAACCTGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptk6 ( XM_017316742.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172231.1 Mus musculus PTK6 protein tyrosine kinase 6 (Ptk6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu80162
Clone ID Related Accession (Same CDS sequence)	NM_001356304.1 , XM_017316742.1
Accession Version	XM_017316742.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	protein-tyrosine kinase 6 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTGCT GGAAGCACAA AACTGAGCCC TTGCCCCACT GGGATGACTG GGAGAGGCCG 
AGGGAGGAGT TCACACTCTG TAAGAAGCTG GGGGCCGGCT ACTTTGGGGA GGTCTTTGAA 
GCGCTCTGGA AAGGCCAGGT CCATGTGGCT GTGAAGGTGA TCTCTAGAGA CAATCTCCTG 
CACCAGCACA CCTTCCAGGC TGAGATTCAG GCCATGAAGA AGCTGCGGCA CAAGCACATC 
CTGTCACTGT ACGCTGTGGC GACTGCAGGG GACCCGGTCT ACATCATCAC GGAGCTCATG 
CCCAAGGGGA ACCTGCTGCA GCTACTGCGT GACTCTGATG AGAAAGCCCT GCCTATTTTG 
GAGCTGGTGG ACTTTGCATC ACAGGTTGCT GAGGGCATGT GCTACCTGGA ATCTCAGAAT 
TACATCCACC GTGACCTGGC TGCAAGGAAC GTTCTTGTTA CAGAGAACAA TCTCTGCAAA 
GTGGGGGACT TTGGGCTTGC CAGGCTTGTC AAGGAGGACA TCTACCTTTC CCATGAGCAC 
AATGTCCCCT ACAAATGGAC AGCACCTGAG GCACTTTCCC GAGGGCATTA CTCCATCAAG 
TCTGATGTCT GGTCTTTTGG AGTTCTTCTT CATGAAATTT TCAGCAGGGG GCAGATGCCC 
TACCCAGGCA TGTCCAATCA TGAAACCTTC CTGAGGGTGG ATGCCGGCTA CCGCATGCCC 
TGCCCTCTGG AGTGCCCACC CAACATACAC AAGCTGATGC TCAGCTGCTG GAGCAGAGAC 
CCCAAGCAGA GACCTTGCTT CAAAGACCTG TGTGAGAAAC TCACAGGTAT CACCAGGTAT 
GAGAACCTGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172231.1
CDS	1597..2451
Translation	MPCWKHKTEPLPHWDDWERPREEFTLCKKLGAGYFGEVFEALWKGQVHVAVKVISRDNLLHQHTFQAEIQAMKKLRHKHILSLYAVATAGDPVYIITELMPKGNLLQLLRDSDEKALPILELVDFASQVAEGMCYLESQNYIHRDLAARNVLVTENNLCKVGDFGLARLVKEDIYLSHEHNVPYKWTAPEALSRGHYSIKSDVWSFGVLLHEIFSRGQMPYPGMSNHETFLRVDAGYRMPCPLECPPNIHKLMLSCWSRDPKQRPCFKDLCEKLTGITRYENLV

Target ORF information:

RefSeq Version	XM_017316742.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus PTK6 protein tyrosine kinase 6 (Ptk6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316742.1

ORF Insert Sequence:

ATGCCCTGCT GGAAGCACAA AACTGAGCCC TTGCCCCACT GGGATGACTG GGAGAGGCCG 
AGGGAGGAGT TCACACTCTG TAAGAAGCTG GGGGCCGGCT ACTTTGGGGA GGTCTTTGAA 
GCGCTCTGGA AAGGCCAGGT CCATGTGGCT GTGAAGGTGA TCTCTAGAGA CAATCTCCTG 
CACCAGCACA CCTTCCAGGC TGAGATTCAG GCCATGAAGA AGCTGCGGCA CAAGCACATC 
CTGTCACTGT ACGCTGTGGC GACTGCAGGG GACCCGGTCT ACATCATCAC GGAGCTCATG 
CCCAAGGGGA ACCTGCTGCA GCTACTGCGT GACTCTGATG AGAAAGCCCT GCCTATTTTG 
GAGCTGGTGG ACTTTGCATC ACAGGTTGCT GAGGGCATGT GCTACCTGGA ATCTCAGAAT 
TACATCCACC GTGACCTGGC TGCAAGGAAC GTTCTTGTTA CAGAGAACAA TCTCTGCAAA 
GTGGGGGACT TTGGGCTTGC CAGGCTTGTC AAGGAGGACA TCTACCTTTC CCATGAGCAC 
AATGTCCCCT ACAAATGGAC AGCACCTGAG GCACTTTCCC GAGGGCATTA CTCCATCAAG 
TCTGATGTCT GGTCTTTTGG AGTTCTTCTT CATGAAATTT TCAGCAGGGG GCAGATGCCC 
TACCCAGGCA TGTCCAATCA TGAAACCTTC CTGAGGGTGG ATGCCGGCTA CCGCATGCCC 
TGCCCTCTGG AGTGCCCACC CAACATACAC AAGCTGATGC TCAGCTGCTG GAGCAGAGAC 
CCCAAGCAGA GACCTTGCTT CAAAGACCTG TGTGAGAAAC TCACAGGTAT CACCAGGTAT 
GAGAACCTGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TNF?/IFN? Mediated Intestinal Epithelial Barrier Dysfunction Is Attenuated by MicroRNA-93 Downregulation of PTK6 in Mouse Colonic Epithelial Cells.
PloS one11(4)e0154351(2016)
Haines RJ,Beard RS Jr,Eitner RA,Chen L,Wu MH

Protein Tyrosine Kinase 6 Regulates UVB-Induced Signaling and Tumorigenesis in Mouse Skin.
The Journal of investigative dermatology135(10)2492-2501(2015 Oct)
Chastkofsky MI,Bie W,Ball-Kell SM,He YY,Tyner AL

Protein tyrosine kinase 6 promotes ERBB2-induced mammary gland tumorigenesis in the mouse.
Cell death & disease6e1848(2015 Aug)
Peng M,Ball-Kell SM,Tyner AL

PTK6 Potentiates Gemcitabine-Induced Apoptosis by Prolonging S-phase and Enhancing DNA Damage in Pancreatic Cancer.
Molecular cancer research : MCR13(8)1174-84(2015 Aug)
Ono H,Basson MD,Ito H

Protein tyrosine kinase 6 mediates TNF?-induced endothelial barrier dysfunction.
Biochemical and biophysical research communications456(1)190-6(2015 Jan)
Haines RJ,Beard RS Jr,Wu MH