Trim30a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trim30a
Entrez Gene ID	20128
Full Name	tripartite motif-containing 30A
Synonyms	Rpt-1,Rpt1,Trim30
General protein information	Preferred Names tripartite motif-containing 30A Names tripartite motif-containing protein 30A down regulatory protein of interleukin-2 receptor regulatory protein, T lymphocyte 1 tripartite motif protein 30 tripartite motif-containing 30 tripartite motif-containing protein 30
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 E3\|7 55.69 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006507471.2	XP_006507534.1	tripartite motif-containing protein 30A isoform X1
NM_009099.2	NP_033125.2	tripartite motif-containing protein 30A
NM_001357467.1	NP_001344396.1	tripartite motif-containing protein 30A

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Trim30a	NP_033125.2
Mus musculus (house mouse)	Trim30d	NP_954597.2
Rattus norvegicus (Norway rat)	Trim30	XP_006229979.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene expression profiles reveal effect of a high-fat diet on the development of white and brown adipose tissues.
Kim HS, Ryoo ZY, Choi SU, Lee S
Gene565(1)15-21(2015 Jul)

TRIM30? Is a Negative-Feedback Regulator of the Intracellular DNA and DNA Virus-Triggered Response by Targeting STING.
Wang Y, Lian Q, Yang B, Yan S, Zhou H, He L, Lin G, Lian Z, Jiang Z, Sun B
PLoS pathogens11(6)e1005012(2015 Jun)

Tripartite motif-containing protein 30 modulates TCR-activated proliferation and effector functions in CD4+ T cells.
Choi UY, Hur JY, Lee MS, Zhang Q, Choi WY, Kim LK, Lee WB, Oh GT, Kim YJ
PloS one9(4)e95805(2014)

Lymphotoxin-beta receptor signalling regulates cytokine expression via TRIM30? in a TRAF3-dependent manner.
Wimmer N, Heigl U, Klingseisen L, Schneider-Brachert W, Hehlgans T
Molecular immunology54(1)40-7(2013 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicated that Trim30 and Ucp3 play pivotal roles in energy balance and glucose homeostasis and might be used as genetic markers to represent the stage of obesity during the early and late stages of adipose tissue development, respectively.
Title: Gene expression profiles reveal effect of a high-fat diet on the development of white and brown adipose tissues.

E3 ligase TRIM30alpha is an important negative-feedback regulator of innate immune responses to DNA viruses by targeting STING
Title: TRIM30? Is a Negative-Feedback Regulator of the Intracellular DNA and DNA Virus-Triggered Response by Targeting STING.

These results indicate a distinct requirement for TRIM30 in modulation of NF-kappaB activation and cell proliferation induced by TCR stimulation.
Title: Tripartite motif-containing protein 30 modulates TCR-activated proliferation and effector functions in CD4+ T cells.

Lymphotoxin-beta receptor signalling regulates cytokine expression via TRIM30alpha in a TRAF3-dependent manner.
Title: Lymphotoxin-beta receptor signalling regulates cytokine expression via TRIM30? in a TRAF3-dependent manner.

LTbetaR activation on macrophages controls the pro-inflammatory response by activation of a TRIM30alpha-dependent signaling pathway
Title: Lymphotoxin-beta receptor activation on macrophages ameliorates acute DSS-induced intestinal inflammation in a TRIM30?-dependent manner.

The following Trim30a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trim30a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20857	NM_009099.2 Latest version!	Mus musculus tripartite motif-containing 30A (Trim30a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu20857	NM_001357467.1 Latest version!	Mus musculus tripartite motif-containing 30A (Trim30a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu20857	XM_006507471.2 Latest version!	Mus musculus tripartite motif-containing 30A (Trim30a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trim30a ( NM_009099.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033125.2 Mus musculus tripartite motif-containing 30A (Trim30a), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu20857
Clone ID Related Accession (Same CDS sequence)	XM_006507471.2 , NM_009099.2 , NM_001357467.1
Accession Version	NM_009099.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	tripartite motif-containing protein 30A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK137506.1, AK137592.1 and DV644722.1. On Feb 8, 2007 this sequence version replaced NM_009099.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC005447.1, SRR1660819.190170.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849385, SAMN01164135 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTCAT CAGTCCTGGA GATGATAAAG GAGGAAGTAA CCTGTCCTAT CTGTTTGGAG 
CTCCTGAAGG AACCTGTGAG TGCTGATTGT AACCACAGCT TCTGCAGAGC CTGCATCACA 
CTGAATTATG AGTCCAACAG AAACACAGAC GGGAAGGGCA ACTGCCCTGT ATGCCGAGTT 
CCTTACCCAT TTGGGAATCT GAGGCCTAAT CTACATGTGG CCAACATAGT AGAGAGGCTC 
AAGGGATTCA AGTCCATTCC AGAGGAGGAG CAGAAGGTGA ATATCTGTGC ACAACATGGA 
GAGAAACTCC GGCTCTTCTG TAGGAAGGAC ATGATGGTCA TCTGCTGGCT TTGTGAGCGA 
TCTCAGGAGC ACCGTGGTCA CCAAACAGCT CTCATTGAAG AGGTTGACCA AGAATACAAG 
GAGAAGCTGC AGGGAGCTCT GTGGAAGCTG ATGAAAAAGG CAAAAATATG TGATGAATGG 
CAGGATGACC TTCAACTGCA GAGAGTTGAC TGGGAGAACC AAATACAGAT CAATGTAGAA 
AATGTTCAGA GACAGTTTAA AGGACTAAGA GACCTCCTGG ACTCCAAGGA GAATGAGGAG 
CTGCAGAAGC TGAAGAAAGA GAAGAAAGAG GTTATGGAAA AGCTGGAAGA GTCTGAAAAT 
GAGCTGGAGG ATCAGACAGA GTTGGTGAGA GACCTCATCT CAGATGTGGA ACATCATTTG 
GAGCTCTCAA CCTTAGAAAT GCTGCAGGGT GCAAATTGTG TCCTGAGAAG GAGTCAGTCC 
TTAAGCCTGC AACAGCCCCA AACTGTCCCC CAAAAGAGAA AAAGAACATT CCAAGCTCCA 
GATCTGAAAG GCATGCTGCA AGTGTATCAA GGACTCATGG ATATCCAGCA ATACTGGGTT 
CATATGACTC TACATGCAAG GAACAATGCA GTCATTGCCA TTAACAAAGA AAAAAGACAA 
ATACAGTATA GAAGTTACAA TACGGTTCCA GTTTCTGAGA TCTACCATTT GGGTGTCCTG 
GGATATCCAG CTCTTTCCTC AGGGAAGCAT TACTGGGAAG TAGACATATC TAGAAGTGAT 
GCCTGGCTCC TCGGATTAAA TGACGGAAAG TGTGCTCAAC CCCAACTTCA CTCAAAGGAA 
GAAATGGGCA TCAAAAAAAA CCTTCATTCT CAGATCAAAC AAAATGTATT GTTTCAGCCT 
AAATGTGGCT ACTGGGTTAT AGGGATGAAG AATCCGTCTG TATACAAGGC CTTTGATGAG 
TGTTCTATCA CCCACAATTC CAGTATCCTG GTCATCTCTC TGCCTGATCG TCCCAGTCGT 
GTCGGAGTTT TCCTGGATCG GAAAGCTGGC ACTCTCTCAT TTTATGATGT TTCTAACTGC 
GGTGCTCTCA TCTATAGGTT CTATGACCCT GCCTTCCCTG TTGAAGTCTA TCCATATTTT 
AATCCTATGA AATGTTCAGA GCCAATGACT ATATGCGGGC CACCCTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033125.2
CDS	229..1719
Translation	MASSVLEMIKEEVTCPICLELLKEPVSADCNHSFCRACITLNYESNRNTDGKGNCPVCRVPYPFGNLRPNLHVANIVERLKGFKSIPEEEQKVNICAQHGEKLRLFCRKDMMVICWLCERSQEHRGHQTALIEEVDQEYKEKLQGALWKLMKKAKICDEWQDDLQLQRVDWENQIQINVENVQRQFKGLRDLLDSKENEELQKLKKEKKEVMEKLEESENELEDQTELVRDLISDVEHHLELSTLEMLQGANCVLRRSQSLSLQQPQTVPQKRKRTFQAPDLKGMLQVYQGLMDIQQYWVHMTLHARNNAVIAINKEKRQIQYRSYNTVPVSEIYHLGVLGYPALSSGKHYWEVDISRSDAWLLGLNDGKCAQPQLHSKEEMGIKKNLHSQIKQNVLFQPKCGYWVIGMKNPSVYKAFDECSITHNSSILVISLPDRPSRVGVFLDRKAGTLSFYDVSNCGALIYRFYDPAFPVEVYPYFNPMKCSEPMTICGPPS

Target ORF information:

RefSeq Version	NM_009099.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tripartite motif-containing 30A (Trim30a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009099.2

ORF Insert Sequence:

ATGGCCTCAT CAGTCCTGGA GATGATAAAG GAGGAAGTAA CCTGTCCTAT CTGTTTGGAG 
CTCCTGAAGG AACCTGTGAG TGCTGATTGT AACCACAGCT TCTGCAGAGC CTGCATCACA 
CTGAATTATG AGTCCAACAG AAACACAGAC GGGAAGGGCA ACTGCCCTGT ATGCCGAGTT 
CCTTACCCAT TTGGGAATCT GAGGCCTAAT CTACATGTGG CCAACATAGT AGAGAGGCTC 
AAGGGATTCA AGTCCATTCC AGAGGAGGAG CAGAAGGTGA ATATCTGTGC ACAACATGGA 
GAGAAACTCC GGCTCTTCTG TAGGAAGGAC ATGATGGTCA TCTGCTGGCT TTGTGAGCGA 
TCTCAGGAGC ACCGTGGTCA CCAAACAGCT CTCATTGAAG AGGTTGACCA AGAATACAAG 
GAGAAGCTGC AGGGAGCTCT GTGGAAGCTG ATGAAAAAGG CAAAAATATG TGATGAATGG 
CAGGATGACC TTCAACTGCA GAGAGTTGAC TGGGAGAACC AAATACAGAT CAATGTAGAA 
AATGTTCAGA GACAGTTTAA AGGACTAAGA GACCTCCTGG ACTCCAAGGA GAATGAGGAG 
CTGCAGAAGC TGAAGAAAGA GAAGAAAGAG GTTATGGAAA AGCTGGAAGA GTCTGAAAAT 
GAGCTGGAGG ATCAGACAGA GTTGGTGAGA GACCTCATCT CAGATGTGGA ACATCATTTG 
GAGCTCTCAA CCTTAGAAAT GCTGCAGGGT GCAAATTGTG TCCTGAGAAG GAGTCAGTCC 
TTAAGCCTGC AACAGCCCCA AACTGTCCCC CAAAAGAGAA AAAGAACATT CCAAGCTCCA 
GATCTGAAAG GCATGCTGCA AGTGTATCAA GGACTCATGG ATATCCAGCA ATACTGGGTT 
CATATGACTC TACATGCAAG GAACAATGCA GTCATTGCCA TTAACAAAGA AAAAAGACAA 
ATACAGTATA GAAGTTACAA TACGGTTCCA GTTTCTGAGA TCTACCATTT GGGTGTCCTG 
GGATATCCAG CTCTTTCCTC AGGGAAGCAT TACTGGGAAG TAGACATATC TAGAAGTGAT 
GCCTGGCTCC TCGGATTAAA TGACGGAAAG TGTGCTCAAC CCCAACTTCA CTCAAAGGAA 
GAAATGGGCA TCAAAAAAAA CCTTCATTCT CAGATCAAAC AAAATGTATT GTTTCAGCCT 
AAATGTGGCT ACTGGGTTAT AGGGATGAAG AATCCGTCTG TATACAAGGC CTTTGATGAG 
TGTTCTATCA CCCACAATTC CAGTATCCTG GTCATCTCTC TGCCTGATCG TCCCAGTCGT 
GTCGGAGTTT TCCTGGATCG GAAAGCTGGC ACTCTCTCAT TTTATGATGT TTCTAACTGC 
GGTGCTCTCA TCTATAGGTT CTATGACCCT GCCTTCCCTG TTGAAGTCTA TCCATATTTT 
AATCCTATGA AATGTTCAGA GCCAATGACT ATATGCGGGC CACCCTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trim30a ( NM_001357467.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001344396.1 Mus musculus tripartite motif-containing 30A (Trim30a), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu20857
Clone ID Related Accession (Same CDS sequence)	XM_006507471.2 , NM_009099.2 , NM_001357467.1
Accession Version	NM_001357467.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	tripartite motif-containing protein 30A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC122400.4 and AC142110.4. On Oct 28, 2017 this sequence version replaced XM_006507471.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.148809.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCCTCAT CAGTCCTGGA GATGATAAAG GAGGAAGTAA CCTGTCCTAT CTGTTTGGAG 
CTCCTGAAGG AACCTGTGAG TGCTGATTGT AACCACAGCT TCTGCAGAGC CTGCATCACA 
CTGAATTATG AGTCCAACAG AAACACAGAC GGGAAGGGCA ACTGCCCTGT ATGCCGAGTT 
CCTTACCCAT TTGGGAATCT GAGGCCTAAT CTACATGTGG CCAACATAGT AGAGAGGCTC 
AAGGGATTCA AGTCCATTCC AGAGGAGGAG CAGAAGGTGA ATATCTGTGC ACAACATGGA 
GAGAAACTCC GGCTCTTCTG TAGGAAGGAC ATGATGGTCA TCTGCTGGCT TTGTGAGCGA 
TCTCAGGAGC ACCGTGGTCA CCAAACAGCT CTCATTGAAG AGGTTGACCA AGAATACAAG 
GAGAAGCTGC AGGGAGCTCT GTGGAAGCTG ATGAAAAAGG CAAAAATATG TGATGAATGG 
CAGGATGACC TTCAACTGCA GAGAGTTGAC TGGGAGAACC AAATACAGAT CAATGTAGAA 
AATGTTCAGA GACAGTTTAA AGGACTAAGA GACCTCCTGG ACTCCAAGGA GAATGAGGAG 
CTGCAGAAGC TGAAGAAAGA GAAGAAAGAG GTTATGGAAA AGCTGGAAGA GTCTGAAAAT 
GAGCTGGAGG ATCAGACAGA GTTGGTGAGA GACCTCATCT CAGATGTGGA ACATCATTTG 
GAGCTCTCAA CCTTAGAAAT GCTGCAGGGT GCAAATTGTG TCCTGAGAAG GAGTCAGTCC 
TTAAGCCTGC AACAGCCCCA AACTGTCCCC CAAAAGAGAA AAAGAACATT CCAAGCTCCA 
GATCTGAAAG GCATGCTGCA AGTGTATCAA GGACTCATGG ATATCCAGCA ATACTGGGTT 
CATATGACTC TACATGCAAG GAACAATGCA GTCATTGCCA TTAACAAAGA AAAAAGACAA 
ATACAGTATA GAAGTTACAA TACGGTTCCA GTTTCTGAGA TCTACCATTT GGGTGTCCTG 
GGATATCCAG CTCTTTCCTC AGGGAAGCAT TACTGGGAAG TAGACATATC TAGAAGTGAT 
GCCTGGCTCC TCGGATTAAA TGACGGAAAG TGTGCTCAAC CCCAACTTCA CTCAAAGGAA 
GAAATGGGCA TCAAAAAAAA CCTTCATTCT CAGATCAAAC AAAATGTATT GTTTCAGCCT 
AAATGTGGCT ACTGGGTTAT AGGGATGAAG AATCCGTCTG TATACAAGGC CTTTGATGAG 
TGTTCTATCA CCCACAATTC CAGTATCCTG GTCATCTCTC TGCCTGATCG TCCCAGTCGT 
GTCGGAGTTT TCCTGGATCG GAAAGCTGGC ACTCTCTCAT TTTATGATGT TTCTAACTGC 
GGTGCTCTCA TCTATAGGTT CTATGACCCT GCCTTCCCTG TTGAAGTCTA TCCATATTTT 
AATCCTATGA AATGTTCAGA GCCAATGACT ATATGCGGGC CACCCTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344396.1
CDS	267..1757
Translation	MASSVLEMIKEEVTCPICLELLKEPVSADCNHSFCRACITLNYESNRNTDGKGNCPVCRVPYPFGNLRPNLHVANIVERLKGFKSIPEEEQKVNICAQHGEKLRLFCRKDMMVICWLCERSQEHRGHQTALIEEVDQEYKEKLQGALWKLMKKAKICDEWQDDLQLQRVDWENQIQINVENVQRQFKGLRDLLDSKENEELQKLKKEKKEVMEKLEESENELEDQTELVRDLISDVEHHLELSTLEMLQGANCVLRRSQSLSLQQPQTVPQKRKRTFQAPDLKGMLQVYQGLMDIQQYWVHMTLHARNNAVIAINKEKRQIQYRSYNTVPVSEIYHLGVLGYPALSSGKHYWEVDISRSDAWLLGLNDGKCAQPQLHSKEEMGIKKNLHSQIKQNVLFQPKCGYWVIGMKNPSVYKAFDECSITHNSSILVISLPDRPSRVGVFLDRKAGTLSFYDVSNCGALIYRFYDPAFPVEVYPYFNPMKCSEPMTICGPPS

Target ORF information:

RefSeq Version	NM_001357467.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tripartite motif-containing 30A (Trim30a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357467.1

ORF Insert Sequence:

ATGGCCTCAT CAGTCCTGGA GATGATAAAG GAGGAAGTAA CCTGTCCTAT CTGTTTGGAG 
CTCCTGAAGG AACCTGTGAG TGCTGATTGT AACCACAGCT TCTGCAGAGC CTGCATCACA 
CTGAATTATG AGTCCAACAG AAACACAGAC GGGAAGGGCA ACTGCCCTGT ATGCCGAGTT 
CCTTACCCAT TTGGGAATCT GAGGCCTAAT CTACATGTGG CCAACATAGT AGAGAGGCTC 
AAGGGATTCA AGTCCATTCC AGAGGAGGAG CAGAAGGTGA ATATCTGTGC ACAACATGGA 
GAGAAACTCC GGCTCTTCTG TAGGAAGGAC ATGATGGTCA TCTGCTGGCT TTGTGAGCGA 
TCTCAGGAGC ACCGTGGTCA CCAAACAGCT CTCATTGAAG AGGTTGACCA AGAATACAAG 
GAGAAGCTGC AGGGAGCTCT GTGGAAGCTG ATGAAAAAGG CAAAAATATG TGATGAATGG 
CAGGATGACC TTCAACTGCA GAGAGTTGAC TGGGAGAACC AAATACAGAT CAATGTAGAA 
AATGTTCAGA GACAGTTTAA AGGACTAAGA GACCTCCTGG ACTCCAAGGA GAATGAGGAG 
CTGCAGAAGC TGAAGAAAGA GAAGAAAGAG GTTATGGAAA AGCTGGAAGA GTCTGAAAAT 
GAGCTGGAGG ATCAGACAGA GTTGGTGAGA GACCTCATCT CAGATGTGGA ACATCATTTG 
GAGCTCTCAA CCTTAGAAAT GCTGCAGGGT GCAAATTGTG TCCTGAGAAG GAGTCAGTCC 
TTAAGCCTGC AACAGCCCCA AACTGTCCCC CAAAAGAGAA AAAGAACATT CCAAGCTCCA 
GATCTGAAAG GCATGCTGCA AGTGTATCAA GGACTCATGG ATATCCAGCA ATACTGGGTT 
CATATGACTC TACATGCAAG GAACAATGCA GTCATTGCCA TTAACAAAGA AAAAAGACAA 
ATACAGTATA GAAGTTACAA TACGGTTCCA GTTTCTGAGA TCTACCATTT GGGTGTCCTG 
GGATATCCAG CTCTTTCCTC AGGGAAGCAT TACTGGGAAG TAGACATATC TAGAAGTGAT 
GCCTGGCTCC TCGGATTAAA TGACGGAAAG TGTGCTCAAC CCCAACTTCA CTCAAAGGAA 
GAAATGGGCA TCAAAAAAAA CCTTCATTCT CAGATCAAAC AAAATGTATT GTTTCAGCCT 
AAATGTGGCT ACTGGGTTAT AGGGATGAAG AATCCGTCTG TATACAAGGC CTTTGATGAG 
TGTTCTATCA CCCACAATTC CAGTATCCTG GTCATCTCTC TGCCTGATCG TCCCAGTCGT 
GTCGGAGTTT TCCTGGATCG GAAAGCTGGC ACTCTCTCAT TTTATGATGT TTCTAACTGC 
GGTGCTCTCA TCTATAGGTT CTATGACCCT GCCTTCCCTG TTGAAGTCTA TCCATATTTT 
AATCCTATGA AATGTTCAGA GCCAATGACT ATATGCGGGC CACCCTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trim30a ( XM_006507471.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507534.1 Mus musculus tripartite motif-containing 30A (Trim30a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu20857
Clone ID Related Accession (Same CDS sequence)	XM_006507471.2 , NM_009099.2 , NM_001357467.1
Accession Version	XM_006507471.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	tripartite motif-containing protein 30A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006507471.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAT CAGTCCTGGA GATGATAAAG GAGGAAGTAA CCTGTCCTAT CTGTTTGGAG 
CTCCTGAAGG AACCTGTGAG TGCTGATTGT AACCACAGCT TCTGCAGAGC CTGCATCACA 
CTGAATTATG AGTCCAACAG AAACACAGAC GGGAAGGGCA ACTGCCCTGT ATGCCGAGTT 
CCTTACCCAT TTGGGAATCT GAGGCCTAAT CTACATGTGG CCAACATAGT AGAGAGGCTC 
AAGGGATTCA AGTCCATTCC AGAGGAGGAG CAGAAGGTGA ATATCTGTGC ACAACATGGA 
GAGAAACTCC GGCTCTTCTG TAGGAAGGAC ATGATGGTCA TCTGCTGGCT TTGTGAGCGA 
TCTCAGGAGC ACCGTGGTCA CCAAACAGCT CTCATTGAAG AGGTTGACCA AGAATACAAG 
GAGAAGCTGC AGGGAGCTCT GTGGAAGCTG ATGAAAAAGG CAAAAATATG TGATGAATGG 
CAGGATGACC TTCAACTGCA GAGAGTTGAC TGGGAGAACC AAATACAGAT CAATGTAGAA 
AATGTTCAGA GACAGTTTAA AGGACTAAGA GACCTCCTGG ACTCCAAGGA GAATGAGGAG 
CTGCAGAAGC TGAAGAAAGA GAAGAAAGAG GTTATGGAAA AGCTGGAAGA GTCTGAAAAT 
GAGCTGGAGG ATCAGACAGA GTTGGTGAGA GACCTCATCT CAGATGTGGA ACATCATTTG 
GAGCTCTCAA CCTTAGAAAT GCTGCAGGGT GCAAATTGTG TCCTGAGAAG GAGTCAGTCC 
TTAAGCCTGC AACAGCCCCA AACTGTCCCC CAAAAGAGAA AAAGAACATT CCAAGCTCCA 
GATCTGAAAG GCATGCTGCA AGTGTATCAA GGACTCATGG ATATCCAGCA ATACTGGGTT 
CATATGACTC TACATGCAAG GAACAATGCA GTCATTGCCA TTAACAAAGA AAAAAGACAA 
ATACAGTATA GAAGTTACAA TACGGTTCCA GTTTCTGAGA TCTACCATTT GGGTGTCCTG 
GGATATCCAG CTCTTTCCTC AGGGAAGCAT TACTGGGAAG TAGACATATC TAGAAGTGAT 
GCCTGGCTCC TCGGATTAAA TGACGGAAAG TGTGCTCAAC CCCAACTTCA CTCAAAGGAA 
GAAATGGGCA TCAAAAAAAA CCTTCATTCT CAGATCAAAC AAAATGTATT GTTTCAGCCT 
AAATGTGGCT ACTGGGTTAT AGGGATGAAG AATCCGTCTG TATACAAGGC CTTTGATGAG 
TGTTCTATCA CCCACAATTC CAGTATCCTG GTCATCTCTC TGCCTGATCG TCCCAGTCGT 
GTCGGAGTTT TCCTGGATCG GAAAGCTGGC ACTCTCTCAT TTTATGATGT TTCTAACTGC 
GGTGCTCTCA TCTATAGGTT CTATGACCCT GCCTTCCCTG TTGAAGTCTA TCCATATTTT 
AATCCTATGA AATGTTCAGA GCCAATGACT ATATGCGGGC CACCCTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507534.1
CDS	312..1802
Translation	MASSVLEMIKEEVTCPICLELLKEPVSADCNHSFCRACITLNYESNRNTDGKGNCPVCRVPYPFGNLRPNLHVANIVERLKGFKSIPEEEQKVNICAQHGEKLRLFCRKDMMVICWLCERSQEHRGHQTALIEEVDQEYKEKLQGALWKLMKKAKICDEWQDDLQLQRVDWENQIQINVENVQRQFKGLRDLLDSKENEELQKLKKEKKEVMEKLEESENELEDQTELVRDLISDVEHHLELSTLEMLQGANCVLRRSQSLSLQQPQTVPQKRKRTFQAPDLKGMLQVYQGLMDIQQYWVHMTLHARNNAVIAINKEKRQIQYRSYNTVPVSEIYHLGVLGYPALSSGKHYWEVDISRSDAWLLGLNDGKCAQPQLHSKEEMGIKKNLHSQIKQNVLFQPKCGYWVIGMKNPSVYKAFDECSITHNSSILVISLPDRPSRVGVFLDRKAGTLSFYDVSNCGALIYRFYDPAFPVEVYPYFNPMKCSEPMTICGPPS

Target ORF information:

RefSeq Version	XM_006507471.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus tripartite motif-containing 30A (Trim30a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507471.2

ORF Insert Sequence:

ATGGCCTCAT CAGTCCTGGA GATGATAAAG GAGGAAGTAA CCTGTCCTAT CTGTTTGGAG 
CTCCTGAAGG AACCTGTGAG TGCTGATTGT AACCACAGCT TCTGCAGAGC CTGCATCACA 
CTGAATTATG AGTCCAACAG AAACACAGAC GGGAAGGGCA ACTGCCCTGT ATGCCGAGTT 
CCTTACCCAT TTGGGAATCT GAGGCCTAAT CTACATGTGG CCAACATAGT AGAGAGGCTC 
AAGGGATTCA AGTCCATTCC AGAGGAGGAG CAGAAGGTGA ATATCTGTGC ACAACATGGA 
GAGAAACTCC GGCTCTTCTG TAGGAAGGAC ATGATGGTCA TCTGCTGGCT TTGTGAGCGA 
TCTCAGGAGC ACCGTGGTCA CCAAACAGCT CTCATTGAAG AGGTTGACCA AGAATACAAG 
GAGAAGCTGC AGGGAGCTCT GTGGAAGCTG ATGAAAAAGG CAAAAATATG TGATGAATGG 
CAGGATGACC TTCAACTGCA GAGAGTTGAC TGGGAGAACC AAATACAGAT CAATGTAGAA 
AATGTTCAGA GACAGTTTAA AGGACTAAGA GACCTCCTGG ACTCCAAGGA GAATGAGGAG 
CTGCAGAAGC TGAAGAAAGA GAAGAAAGAG GTTATGGAAA AGCTGGAAGA GTCTGAAAAT 
GAGCTGGAGG ATCAGACAGA GTTGGTGAGA GACCTCATCT CAGATGTGGA ACATCATTTG 
GAGCTCTCAA CCTTAGAAAT GCTGCAGGGT GCAAATTGTG TCCTGAGAAG GAGTCAGTCC 
TTAAGCCTGC AACAGCCCCA AACTGTCCCC CAAAAGAGAA AAAGAACATT CCAAGCTCCA 
GATCTGAAAG GCATGCTGCA AGTGTATCAA GGACTCATGG ATATCCAGCA ATACTGGGTT 
CATATGACTC TACATGCAAG GAACAATGCA GTCATTGCCA TTAACAAAGA AAAAAGACAA 
ATACAGTATA GAAGTTACAA TACGGTTCCA GTTTCTGAGA TCTACCATTT GGGTGTCCTG 
GGATATCCAG CTCTTTCCTC AGGGAAGCAT TACTGGGAAG TAGACATATC TAGAAGTGAT 
GCCTGGCTCC TCGGATTAAA TGACGGAAAG TGTGCTCAAC CCCAACTTCA CTCAAAGGAA 
GAAATGGGCA TCAAAAAAAA CCTTCATTCT CAGATCAAAC AAAATGTATT GTTTCAGCCT 
AAATGTGGCT ACTGGGTTAT AGGGATGAAG AATCCGTCTG TATACAAGGC CTTTGATGAG 
TGTTCTATCA CCCACAATTC CAGTATCCTG GTCATCTCTC TGCCTGATCG TCCCAGTCGT 
GTCGGAGTTT TCCTGGATCG GAAAGCTGGC ACTCTCTCAT TTTATGATGT TTCTAACTGC 
GGTGCTCTCA TCTATAGGTT CTATGACCCT GCCTTCCCTG TTGAAGTCTA TCCATATTTT 
AATCCTATGA AATGTTCAGA GCCAATGACT ATATGCGGGC CACCCTCCTA A