Ptprn2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ptprn2
Entrez Gene ID	19276
Full Name	protein tyrosine phosphatase, receptor type, N polypeptide 2
Synonyms	4930425H11Rik,IA2beta,PTP-NP,Phol,mKIAA0387,phogrin
General protein information	Preferred Names protein tyrosine phosphatase, receptor type, N polypeptide 2 Names receptor-type tyrosine-protein phosphatase N2 IA-2 beta PTP IA-2beta R-PTP-N2 protein tyrosine phosphatase-NP
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12 F2\|12 62.65 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_011244019.3	XP_011242321.1	receptor-type tyrosine-protein phosphatase N2 isoform X1
XM_011244019.2	XP_011242321.1	receptor-type tyrosine-protein phosphatase N2 isoform X1
NM_011215.2	NP_035345.2	receptor-type tyrosine-protein phosphatase N2 precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ptprn2	NP_001122139.1
Homo sapiens (human)	PTPRN2	NP_002838.2
Rattus norvegicus (Norway rat)	Ptprn2	NP_113788.1
Canis lupus familiaris (dog)	PTPRN2	XP_005629771.1
Mus musculus (house mouse)	Ptprn2	NP_035345.2
Xenopus tropicalis (tropical clawed frog)	ptprn2	XP_002935220.2
Bos taurus (cattle)	PTPRN2	XP_002687034.2
Gallus gallus (chicken)	PTPRN2	XP_418552.4

Gene Ontology
Bibliography

Related articles in PubMed

Pathophysiologic changes in IA-2/IA-2? null mice are secondary to alterations in the secretion of hormones and neurotransmitters.
Cai T, Notkins AL
Acta diabetologica53(1)7-12(2016 Feb)

The Ia-2? intronic miRNA, miR-153, is a negative regulator of insulin and dopamine secretion through its effect on the Cacna1c gene in mice.
Xu H, Abuhatzira L, Carmona GN, Vadrevu S, Satin LS, Notkins AL
Diabetologia58(10)2298-306(2015 Oct)

Multiple microRNAs within the 14q32 cluster target the mRNAs of major type 1 diabetes autoantigens IA-2, IA-2?, and GAD65.
Abuhatzira L, Xu H, Tahhan G, Boulougoura A, Sch?ffer AA, Notkins AL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology29(10)4374-83(2015 Oct)

The dense core vesicle protein IA-2, but not IA-2?, is required for active avoidance learning.
Carmona GN, Nishimura T, Schindler CW, Panlilio LV, Notkins AL
Neuroscience26935-42(2014 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The deletion of IA-2 and IA-2beta results in multiple pathophysiologic changes and represents a unique in vivo model for studying the effect of hormone and neurotransmitter reduction on known and still unrecognized targets.
Title: Pathophysiologic changes in IA-2/IA-2? null mice are secondary to alterations in the secretion of hormones and neurotransmitters.

Data show that miRNAs are involved in regulating the expression of the major type 1 diabetes (T1D) autoantigens IA-2, IA-2beta, and GAD65 enzyme.
Title: Multiple microRNAs within the 14q32 cluster target the mRNAs of major type 1 diabetes autoantigens IA-2, IA-2?, and GAD65.

Membrane-proximal ectodomain of phogrin was produced as a recombinant protein and characterized' results showed that it is a properly folded monomeric domain.
Title: Biophysical characterization of the membrane-proximal ectodomain of the receptor-type protein-tyrosine phosphatase phogrin.

deletion of IA-2 and IA-2beta, structural proteins of secretory vesicles and modulators of neuroendocrine secretion, has a profound effect on the circadian system.
Title: Deletion of the secretory vesicle proteins IA-2 and IA-2beta disrupts circadian rhythms of cardiovascular and physical activity.

Phogrin and IA-2 function as essential regulators of autocrine insulin action in pancreatic beta-cells.
Title: Gene silencing of phogrin unveils its essential role in glucose-responsive pancreatic beta-cell growth.

The following Ptprn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ptprn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu68553	XM_011244019.3 Latest version!	Mus musculus protein tyrosine phosphatase, receptor type, N polypeptide 2 (Ptprn2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu13442	NM_011215.2 Latest version!	Mus musculus protein tyrosine phosphatase, receptor type, N polypeptide 2 (Ptprn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
View Old Accession Versions >>
OMu68553	XM_011244019.2	Mus musculus protein tyrosine phosphatase, receptor type, N polypeptide 2 (Ptprn2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ptprn2 ( XM_011244019.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242321.1 Mus musculus protein tyrosine phosphatase, receptor type, N polypeptide 2 (Ptprn2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu68553
Clone ID Related Accession (Same CDS sequence)	XM_011244019.3 , XM_011244019.2
Accession Version	XM_011244019.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	receptor-type tyrosine-protein phosphatase N2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011244019.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCCGC CGCTCCCGCT TTTGCTGCTG CTGCTGCTAC CGCCGCCGCT GCCTCGCGCT 
CTGCCCGCCC CCGCGTCTGC CCGCGGCCGG CAGCTCCCGG GGCGCCTGGG ATGCTTGTTT 
GAGGATGGCT TGTGTGGATC ACTGGAGACC TGTGTGAACG GTTTCACGTG GCAAGATGAC 
TATACCCAGC GTGTGATTGC CCAGGAGCTT GCAAACCTCC CCAAGGCCTA CCTATGGCAT 
GGGGAAGCGT CCGGCCCAGC CAGGTCCTTA CAACAGAATG CTGACAATGA AAAATGGTTC 
AGTCTGGAGA GGGAGGTGGC CCTGGCCAAG ACCCTTCGGC GCTATCTGCC CTACCTGGAG 
CTTCTGTCCC AGACCCCAAC AGCAAATGCA CACTCTAGGA TAGACCATGA GACTCGTCCA 
GCCAAGGGTG AAGACTCTTC CCCTGAGAAC ATCCTGACCT ACGTGGCCCA CACATCAGCA 
CTGACCTATC CTCCTGCAAC CCGGGCCAAG TATCCTGATA ACCTTCTGCG GCCCTTTAGC 
CGGCTCCAGC CAGATGAGCT CAGCCCCAAG GTAGACGGTG ACATAGACAA ACAGAAGCTG 
ATTGCAGCAC TGGGCGCCTA CACTGCTCAG AGGCTTCCTG GAGAAAATGA CCCAGAGCCA 
CGGTACCTTG TACATGGTTC CGCGAGAGCA CCAAGGCCAT TCTCAGCAAC TGCTTTGTCT 
CAGAGATGGC CTCCACCTCC TGGAGACGCC AAAGACTCCC CGAGTATGGA TGATGACACA 
CTCCTGCAGA GTCTCCTGAA GGATTTGCAG CAGAACTCTG AAGTGGACCG CCTGGGCCCC 
CTGAAGGAGG AGAAAGCAGA CTCAGTTGCT GGAGCCATAC AAAGTGATCC TGCAGAGGGA 
AGCCAAGAAA GCCACGGGAG AGGGGCTGAA GGACAGCCAA GAGAGCAGAC AGATGCCCCA 
GAGACAATGC TTCAAGATCA CAGACTATCA GAGGTGGATG ACCCAGTGTA CAAGGAGGTC 
AACCGTCTGA GCTTCCAGCT TGGGGACCTC TTGAAGGACT ATGGGTCTCC TCTCTTACCT 
GAAGGTCCCC TTCTAGAAAA ATCCTCCAGA GAAGAGATTA AGAAATCAGA GCAGCCAGAG 
GAGGTCTTGT CTTCAGAAGA GGAGACTGCT GGGGTGGAGC ATGTGAGGAG CCGGACTTAC 
TCCAAAGACC TATTTGAAAG GAAACCAAAC TCAGAGCCCC AGCCCAGGAG GCTTGAGGAT 
CAGTTCCAAA ACCGAGCTCC AGAGTTGTGG GAGGATGAAG AAAGCCTCAA ATTGGCAGCA 
CAGGGACCCC CTAGTGGAGG CCTACAGCTG GAAGTGCAGC CTTCTGAGGA ACAGCAGGGA 
TACATCCTCA CAGGAAACAA CCCTCTAAGT CCAGAGAAGG GGAAGCAGCT GATGGACCAA 
GTTGCCCACA TCCTCCGGGT ACCTTCCAGC TTCTTTGCAG ATATCAAAGT TTTGGGACCA 
GCAGTGACCT TCAAAGTAAG TGCCAACATC CAAAACATGA CAACTGCCGA TGTCATCAAG 
GCTGCAGCTG ACAACAAAGA CCAGCTGGAG AAGGCAACTG GACTGACAAT CCTTCAAAGT 
GGAATCAGGC CGAAGGGAAA GCTCAAACTC CTGCCGCATC AGGAAGAGCA AGAGGACTCT 
ACCAAGTTCA TTTTGCTCAC CTTCCTCTCC ATTGCCTGCA TCCTGGGGGT TCTCCTGGCT 
TCCAGCCTTG CCTACTGCCT CCGCCACAAC TCACACTACA AGCTGAAGGA CAAGTTGTCT 
GGACTAGGCG CTGACCCCAG TGCAGATGCC ACTGAAGCCT ACCAGGAGCT ATGCCGCCAG 
CGTATGGCTA TTCGTCCACA GGACCGCTCT GAGGGACCAC ATACATCACG CATCAACAGC 
GTCTCATCCC AGTTCAGCGA TGGGCCGATG CCTAGTCCTT CGGCTCGGAG CAGCACTTCA 
TCCTGGTCTG AGGAGCCTGT CCAGTCCAAC ATGGACATCT CTACTGGCCA CATGATCCTG 
GCCTACATGG AAGACCATCT GAAGAACAAG AACCGCCTGG AGAAGGAGTG GGAAGCACTG 
TGCGCCTACC AAGCAGAGCC CAACAGCTCA CTTGTGGCCC AGAGAGAGGA GAATGCACCC 
AAGAACCGTT CCCTGGCTGT GCTGACCTAT GACCACTCCA GGATCCTGTT GAAGTCTCAA 
AACAGCCATG GCAGTTCCGA CTACATCAAT GCCAGCCCCA TTATGGACCA TGACCCACGA 
AACCCCGCAT ACATTGCCAC CCAAGGCCCA CTTCCCGCCA CGGTGGCCGA CTTCTGGCAG 
ATGGTGTGGG AAAGCGGCTG TGCAGTCATT GTCATGCTGA CACCCCTCTC CGAGAACGGC 
GTCCGGCAGT GCCATCACTA CTGGCCCGAT GAAGGCTCCA ACCTCTACCA TGTCTACGAG 
GTCAATCTAG TCTCTGAGCA CATATGGTGC CAGGATTTCC TGGTGAGAAG CTTTTACCTG 
AAGAACCTGC AGACCAACGA GACTCGCACG GTGACCCAGT TCCACTTCCT GAGTTGGTAT 
GACCAGGGAG TCCCTTCCTC CACGAGGTCA CTCCTGGATT TCCGCAGAAA AGTGAACAAA 
TGCTACCGAG GCCGCTCTTG TCCGATCATT GTCCATTGCA GTGACGGCGC CGGCAGGAGT 
GGAACCTACG TCCTGATTGA CATGGTTCTC AATAAGATGG CCAAAGGTGC TAAAGAGATT 
GATATCGCAG CGACCCTGGA GCACTTGAGG GACCAGAGAC CAGGCATGGT CCAGACAAAG 
GAGCAGTTTG AGTTTGCGCT GACAGCTGTG GCTGAGGAGG TGAATGCCAT CCTGAAGGCC 
CTTCCCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242321.1
CDS	171..3062
Translation	MGPPLPLLLLLLLPPPLPRALPAPASARGRQLPGRLGCLFEDGLCGSLETCVNGFTWQDDYTQRVIAQELANLPKAYLWHGEASGPARSLQQNADNEKWFSLEREVALAKTLRRYLPYLELLSQTPTANAHSRIDHETRPAKGEDSSPENILTYVAHTSALTYPPATRAKYPDNLLRPFSRLQPDELSPKVDGDIDKQKLIAALGAYTAQRLPGENDPEPRYLVHGSARAPRPFSATALSQRWPPPPGDAKDSPSMDDDTLLQSLLKDLQQNSEVDRLGPLKEEKADSVAGAIQSDPAEGSQESHGRGAEGQPREQTDAPETMLQDHRLSEVDDPVYKEVNRLSFQLGDLLKDYGSPLLPEGPLLEKSSREEIKKSEQPEEVLSSEEETAGVEHVRSRTYSKDLFERKPNSEPQPRRLEDQFQNRAPELWEDEESLKLAAQGPPSGGLQLEVQPSEEQQGYILTGNNPLSPEKGKQLMDQVAHILRVPSSFFADIKVLGPAVTFKVSANIQNMTTADVIKAAADNKDQLEKATGLTILQSGIRPKGKLKLLPHQEEQEDSTKFILLTFLSIACILGVLLASSLAYCLRHNSHYKLKDKLSGLGADPSADATEAYQELCRQRMAIRPQDRSEGPHTSRINSVSSQFSDGPMPSPSARSSTSSWSEEPVQSNMDISTGHMILAYMEDHLKNKNRLEKEWEALCAYQAEPNSSLVAQREENAPKNRSLAVLTYDHSRILLKSQNSHGSSDYINASPIMDHDPRNPAYIATQGPLPATVADFWQMVWESGCAVIVMLTPLSENGVRQCHHYWPDEGSNLYHVYEVNLVSEHIWCQDFLVRSFYLKNLQTNETRTVTQFHFLSWYDQGVPSSTRSLLDFRRKVNKCYRGRSCPIIVHCSDGAGRSGTYVLIDMVLNKMAKGAKEIDIAATLEHLRDQRPGMVQTKEQFEFALTAVAEEVNAILKALPQ

Target ORF information:

RefSeq Version	XM_011244019.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein tyrosine phosphatase, receptor type, N polypeptide 2 (Ptprn2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244019.3

ORF Insert Sequence:

ATGGGGCCGC CGCTCCCGCT TTTGCTGCTG CTGCTGCTAC CGCCGCCGCT GCCTCGCGCT 
CTGCCCGCCC CCGCGTCTGC CCGCGGCCGG CAGCTCCCGG GGCGCCTGGG ATGCTTGTTT 
GAGGATGGCT TGTGTGGATC ACTGGAGACC TGTGTGAACG GTTTCACGTG GCAAGATGAC 
TATACCCAGC GTGTGATTGC CCAGGAGCTT GCAAACCTCC CCAAGGCCTA CCTATGGCAT 
GGGGAAGCGT CCGGCCCAGC CAGGTCCTTA CAACAGAATG CTGACAATGA AAAATGGTTC 
AGTCTGGAGA GGGAGGTGGC CCTGGCCAAG ACCCTTCGGC GCTATCTGCC CTACCTGGAG 
CTTCTGTCCC AGACCCCAAC AGCAAATGCA CACTCTAGGA TAGACCATGA GACTCGTCCA 
GCCAAGGGTG AAGACTCTTC CCCTGAGAAC ATCCTGACCT ACGTGGCCCA CACATCAGCA 
CTGACCTATC CTCCTGCAAC CCGGGCCAAG TATCCTGATA ACCTTCTGCG GCCCTTTAGC 
CGGCTCCAGC CAGATGAGCT CAGCCCCAAG GTAGACGGTG ACATAGACAA ACAGAAGCTG 
ATTGCAGCAC TGGGCGCCTA CACTGCTCAG AGGCTTCCTG GAGAAAATGA CCCAGAGCCA 
CGGTACCTTG TACATGGTTC CGCGAGAGCA CCAAGGCCAT TCTCAGCAAC TGCTTTGTCT 
CAGAGATGGC CTCCACCTCC TGGAGACGCC AAAGACTCCC CGAGTATGGA TGATGACACA 
CTCCTGCAGA GTCTCCTGAA GGATTTGCAG CAGAACTCTG AAGTGGACCG CCTGGGCCCC 
CTGAAGGAGG AGAAAGCAGA CTCAGTTGCT GGAGCCATAC AAAGTGATCC TGCAGAGGGA 
AGCCAAGAAA GCCACGGGAG AGGGGCTGAA GGACAGCCAA GAGAGCAGAC AGATGCCCCA 
GAGACAATGC TTCAAGATCA CAGACTATCA GAGGTGGATG ACCCAGTGTA CAAGGAGGTC 
AACCGTCTGA GCTTCCAGCT TGGGGACCTC TTGAAGGACT ATGGGTCTCC TCTCTTACCT 
GAAGGTCCCC TTCTAGAAAA ATCCTCCAGA GAAGAGATTA AGAAATCAGA GCAGCCAGAG 
GAGGTCTTGT CTTCAGAAGA GGAGACTGCT GGGGTGGAGC ATGTGAGGAG CCGGACTTAC 
TCCAAAGACC TATTTGAAAG GAAACCAAAC TCAGAGCCCC AGCCCAGGAG GCTTGAGGAT 
CAGTTCCAAA ACCGAGCTCC AGAGTTGTGG GAGGATGAAG AAAGCCTCAA ATTGGCAGCA 
CAGGGACCCC CTAGTGGAGG CCTACAGCTG GAAGTGCAGC CTTCTGAGGA ACAGCAGGGA 
TACATCCTCA CAGGAAACAA CCCTCTAAGT CCAGAGAAGG GGAAGCAGCT GATGGACCAA 
GTTGCCCACA TCCTCCGGGT ACCTTCCAGC TTCTTTGCAG ATATCAAAGT TTTGGGACCA 
GCAGTGACCT TCAAAGTAAG TGCCAACATC CAAAACATGA CAACTGCCGA TGTCATCAAG 
GCTGCAGCTG ACAACAAAGA CCAGCTGGAG AAGGCAACTG GACTGACAAT CCTTCAAAGT 
GGAATCAGGC CGAAGGGAAA GCTCAAACTC CTGCCGCATC AGGAAGAGCA AGAGGACTCT 
ACCAAGTTCA TTTTGCTCAC CTTCCTCTCC ATTGCCTGCA TCCTGGGGGT TCTCCTGGCT 
TCCAGCCTTG CCTACTGCCT CCGCCACAAC TCACACTACA AGCTGAAGGA CAAGTTGTCT 
GGACTAGGCG CTGACCCCAG TGCAGATGCC ACTGAAGCCT ACCAGGAGCT ATGCCGCCAG 
CGTATGGCTA TTCGTCCACA GGACCGCTCT GAGGGACCAC ATACATCACG CATCAACAGC 
GTCTCATCCC AGTTCAGCGA TGGGCCGATG CCTAGTCCTT CGGCTCGGAG CAGCACTTCA 
TCCTGGTCTG AGGAGCCTGT CCAGTCCAAC ATGGACATCT CTACTGGCCA CATGATCCTG 
GCCTACATGG AAGACCATCT GAAGAACAAG AACCGCCTGG AGAAGGAGTG GGAAGCACTG 
TGCGCCTACC AAGCAGAGCC CAACAGCTCA CTTGTGGCCC AGAGAGAGGA GAATGCACCC 
AAGAACCGTT CCCTGGCTGT GCTGACCTAT GACCACTCCA GGATCCTGTT GAAGTCTCAA 
AACAGCCATG GCAGTTCCGA CTACATCAAT GCCAGCCCCA TTATGGACCA TGACCCACGA 
AACCCCGCAT ACATTGCCAC CCAAGGCCCA CTTCCCGCCA CGGTGGCCGA CTTCTGGCAG 
ATGGTGTGGG AAAGCGGCTG TGCAGTCATT GTCATGCTGA CACCCCTCTC CGAGAACGGC 
GTCCGGCAGT GCCATCACTA CTGGCCCGAT GAAGGCTCCA ACCTCTACCA TGTCTACGAG 
GTCAATCTAG TCTCTGAGCA CATATGGTGC CAGGATTTCC TGGTGAGAAG CTTTTACCTG 
AAGAACCTGC AGACCAACGA GACTCGCACG GTGACCCAGT TCCACTTCCT GAGTTGGTAT 
GACCAGGGAG TCCCTTCCTC CACGAGGTCA CTCCTGGATT TCCGCAGAAA AGTGAACAAA 
TGCTACCGAG GCCGCTCTTG TCCGATCATT GTCCATTGCA GTGACGGCGC CGGCAGGAGT 
GGAACCTACG TCCTGATTGA CATGGTTCTC AATAAGATGG CCAAAGGTGC TAAAGAGATT 
GATATCGCAG CGACCCTGGA GCACTTGAGG GACCAGAGAC CAGGCATGGT CCAGACAAAG 
GAGCAGTTTG AGTTTGCGCT GACAGCTGTG GCTGAGGAGG TGAATGCCAT CCTGAAGGCC 
CTTCCCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptprn2 ( NM_011215.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035345.2 Mus musculus protein tyrosine phosphatase, receptor type, N polypeptide 2 (Ptprn2), mRNA.

Price & Availability↑

CloneID	OMu13442
Clone ID Related Accession (Same CDS sequence)	NM_011215.2
Accession Version	NM_011215.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	receptor-type tyrosine-protein phosphatase N2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC160634.2, AK138655.1, BC133678.1 and AC164641.5. On Apr 1, 2009 this sequence version replaced NM_011215.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U57345.1, BC133678.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849376, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCCGC CGCTCCCGCT TTTGCTGCTG CTGCTGCTAC CGCCGCCGCT GCCTCGCGCT 
CTGCCCGCCC CCGCGTCTGC CCGCGGCCGG CAGCTCCCGG GGCGCCTGGG ATGCTTGTTT 
GAGGATGGCT TGTGTGGATC ACTGGAGACC TGTGTGAACG ATGGTGTGTT TGGAAGATGT 
CAAAAGGTTC CGGTGATGGA CACTTACCGA TATGAGGTAC CACCAGGAGC CCTGCTGCAC 
CTGAAGGTCA CCTTACAGAA GCTCTCCCGT ACAGGTTTCA CGTGGCAAGA TGACTATACC 
CAGCGTGTGA TTGCCCAGGA GCTTGCAAAC CTCCCCAAGG CCTACCTATG GCATGGGGAA 
GCGTCCGGCC CAGCCAGGTC CTTACAACAG AATGCTGACA ATGAAAAATG GTTCAGTCTG 
GAGAGGGAGG TGGCCCTGGC CAAGACCCTT CGGCGCTATC TGCCCTACCT GGAGCTTCTG 
TCCCAGACCC CAACAGCAAA TGCACACTCT AGGATAGACC ATGAGACTCG TCCAGCCAAG 
GGTGAAGACT CTTCCCCTGA GAACATCCTG ACCTACGTGG CCCACACATC AGCACTGACC 
TATCCTCCTG CAACCCGGGC CAAGTATCCT GATAACCTTC TGCGGCCCTT TAGCCGGCTC 
CAGCCAGATG AGCTCAGCCC CAAGGTAGAC GGTGACATAG ACAAACAGAA GCTGATTGCA 
GCACTGGGCG CCTACACTGC TCAGAGGCTT CCTGGAGAAA ATGACCCAGA GCCACGGTAC 
CTTGTACATG GTTCCGCGAG AGCACCAAGG CCATTCTCAG CAACTGCTTT GTCTCAGAGA 
TGGCCTCCAC CTCCTGGAGA CGCCAAAGAC TCCCCGAGTA TGGATGATGA CACACTCCTG 
CAGAGTCTCC TGAAGGATTT GCAGCAGAAC TCTGAAGTGG ACCGCCTGGG CCCCCTGAAG 
GAGGAGAAAG CAGACTCAGT TGCTGGAGCC ATACAAAGTG ATCCTGCAGA GGGAAGCCAA 
GAAAGCCACG GGAGAGGGGC TGAAGGACAG CCAAGAGAGC AGACAGATGC CCCAGAGACA 
ATGCTTCAAG ATCACAGACT ATCAGAGGTG GATGACCCAG TGTACAAGGA GGTCAACCGT 
CTGAGCTTCC AGCTTGGGGA CCTCTTGAAG GACTATGGGT CTCCTCTCTT ACCTGAAGGT 
CCCCTTCTAG AAAAATCCTC CAGAGAAGAG ATTAAGAAAT CAGAGCAGCC AGAGGAGGTC 
TTGTCTTCAG AAGAGGAGAC TGCTGGGGTG GAGCATGTGA GGAGCCGGAC TTACTCCAAA 
GACCTATTTG AAAGGAAACC AAACTCAGAG CCCCAGCCCA GGAGGCTTGA GGATCAGTTC 
CAAAACCGAG CTCCAGAGTT GTGGGAGGAT GAAGAAAGCC TCAAATTGGC AGCACAGGGA 
CCCCCTAGTG GAGGCCTACA GCTGGAAGTG CAGCCTTCTG AGGAACAGCA GGGATACATC 
CTCACAGGAA ACAACCCTCT AAGTCCAGAG AAGGGGAAGC AGCTGATGGA CCAAGTTGCC 
CACATCCTCC GGGTACCTTC CAGCTTCTTT GCAGATATCA AAGTTTTGGG ACCAGCAGTG 
ACCTTCAAAG TAAGTGCCAA CATCCAAAAC ATGACAACTG CCGATGTCAT CAAGGCTGCA 
GCTGACAACA AAGACCAGCT GGAGAAGGCA ACTGGACTGA CAATCCTTCA AAGTGGAATC 
AGGCCGAAGG GAAAGCTCAA ACTCCTGCCG CATCAGGAAG AGCAAGAGGA CTCTACCAAG 
TTCATTTTGC TCACCTTCCT CTCCATTGCC TGCATCCTGG GGGTTCTCCT GGCTTCCAGC 
CTTGCCTACT GCCTCCGCCA CAACTCACAC TACAAGCTGA AGGACAAGTT GTCTGGACTA 
GGCGCTGACC CCAGTGCAGA TGCCACTGAA GCCTACCAGG AGCTATGCCG CCAGCGTATG 
GCTATTCGTC CACAGGACCG CTCTGAGGGA CCACATACAT CACGCATCAA CAGCGTCTCA 
TCCCAGTTCA GCGATGGGCC GATGCCTAGT CCTTCGGCTC GGAGCAGCAC TTCATCCTGG 
TCTGAGGAGC CTGTCCAGTC CAACATGGAC ATCTCTACTG GCCACATGAT CCTGGCCTAC 
ATGGAAGACC ATCTGAAGAA CAAGAACCGC CTGGAGAAGG AGTGGGAAGC ACTGTGCGCC 
TACCAAGCAG AGCCCAACAG CTCACTTGTG GCCCAGAGAG AGGAGAATGC ACCCAAGAAC 
CGTTCCCTGG CTGTGCTGAC CTATGACCAC TCCAGGATCC TGTTGAAGTC TCAAAACAGC 
CATGGCAGTT CCGACTACAT CAATGCCAGC CCCATTATGG ACCATGACCC ACGAAACCCC 
GCATACATTG CCACCCAAGG CCCACTTCCC GCCACGGTGG CCGACTTCTG GCAGATGGTG 
TGGGAAAGCG GCTGTGCAGT CATTGTCATG CTGACACCCC TCTCCGAGAA CGGCGTCCGG 
CAGTGCCATC ACTACTGGCC CGATGAAGGC TCCAACCTCT ACCATGTCTA CGAGGTCAAT 
CTAGTCTCTG AGCACATATG GTGCCAGGAT TTCCTGGTGA GAAGCTTTTA CCTGAAGAAC 
CTGCAGACCA ACGAGACTCG CACGGTGACC CAGTTCCACT TCCTGAGTTG GTATGACCAG 
GGAGTCCCTT CCTCCACGAG GTCACTCCTG GATTTCCGCA GAAAAGTGAA CAAATGCTAC 
CGAGGCCGCT CTTGTCCGAT CATTGTCCAT TGCAGTGACG GCGCCGGCAG GAGTGGAACC 
TACGTCCTGA TTGACATGGT TCTCAATAAG ATGGCCAAAG GTGCTAAAGA GATTGATATC 
GCAGCGACCC TGGAGCACTT GAGGGACCAG AGACCAGGCA TGGTCCAGAC AAAGGAGCAG 
TTTGAGTTTG CGCTGACAGC TGTGGCTGAG GAGGTGAATG CCATCCTGAA GGCCCTTCCC 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035345.2
CDS	146..3151
Translation	MGPPLPLLLLLLLPPPLPRALPAPASARGRQLPGRLGCLFEDGLCGSLETCVNDGVFGRCQKVPVMDTYRYEVPPGALLHLKVTLQKLSRTGFTWQDDYTQRVIAQELANLPKAYLWHGEASGPARSLQQNADNEKWFSLEREVALAKTLRRYLPYLELLSQTPTANAHSRIDHETRPAKGEDSSPENILTYVAHTSALTYPPATRAKYPDNLLRPFSRLQPDELSPKVDGDIDKQKLIAALGAYTAQRLPGENDPEPRYLVHGSARAPRPFSATALSQRWPPPPGDAKDSPSMDDDTLLQSLLKDLQQNSEVDRLGPLKEEKADSVAGAIQSDPAEGSQESHGRGAEGQPREQTDAPETMLQDHRLSEVDDPVYKEVNRLSFQLGDLLKDYGSPLLPEGPLLEKSSREEIKKSEQPEEVLSSEEETAGVEHVRSRTYSKDLFERKPNSEPQPRRLEDQFQNRAPELWEDEESLKLAAQGPPSGGLQLEVQPSEEQQGYILTGNNPLSPEKGKQLMDQVAHILRVPSSFFADIKVLGPAVTFKVSANIQNMTTADVIKAAADNKDQLEKATGLTILQSGIRPKGKLKLLPHQEEQEDSTKFILLTFLSIACILGVLLASSLAYCLRHNSHYKLKDKLSGLGADPSADATEAYQELCRQRMAIRPQDRSEGPHTSRINSVSSQFSDGPMPSPSARSSTSSWSEEPVQSNMDISTGHMILAYMEDHLKNKNRLEKEWEALCAYQAEPNSSLVAQREENAPKNRSLAVLTYDHSRILLKSQNSHGSSDYINASPIMDHDPRNPAYIATQGPLPATVADFWQMVWESGCAVIVMLTPLSENGVRQCHHYWPDEGSNLYHVYEVNLVSEHIWCQDFLVRSFYLKNLQTNETRTVTQFHFLSWYDQGVPSSTRSLLDFRRKVNKCYRGRSCPIIVHCSDGAGRSGTYVLIDMVLNKMAKGAKEIDIAATLEHLRDQRPGMVQTKEQFEFALTAVAEEVNAILKALPQ

Target ORF information:

RefSeq Version	NM_011215.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein tyrosine phosphatase, receptor type, N polypeptide 2 (Ptprn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011215.2

ORF Insert Sequence:

ATGGGGCCGC CGCTCCCGCT TTTGCTGCTG CTGCTGCTAC CGCCGCCGCT GCCTCGCGCT 
CTGCCCGCCC CCGCGTCTGC CCGCGGCCGG CAGCTCCCGG GGCGCCTGGG ATGCTTGTTT 
GAGGATGGCT TGTGTGGATC ACTGGAGACC TGTGTGAACG ATGGTGTGTT TGGAAGATGT 
CAAAAGGTTC CGGTGATGGA CACTTACCGA TATGAGGTAC CACCAGGAGC CCTGCTGCAC 
CTGAAGGTCA CCTTACAGAA GCTCTCCCGT ACAGGTTTCA CGTGGCAAGA TGACTATACC 
CAGCGTGTGA TTGCCCAGGA GCTTGCAAAC CTCCCCAAGG CCTACCTATG GCATGGGGAA 
GCGTCCGGCC CAGCCAGGTC CTTACAACAG AATGCTGACA ATGAAAAATG GTTCAGTCTG 
GAGAGGGAGG TGGCCCTGGC CAAGACCCTT CGGCGCTATC TGCCCTACCT GGAGCTTCTG 
TCCCAGACCC CAACAGCAAA TGCACACTCT AGGATAGACC ATGAGACTCG TCCAGCCAAG 
GGTGAAGACT CTTCCCCTGA GAACATCCTG ACCTACGTGG CCCACACATC AGCACTGACC 
TATCCTCCTG CAACCCGGGC CAAGTATCCT GATAACCTTC TGCGGCCCTT TAGCCGGCTC 
CAGCCAGATG AGCTCAGCCC CAAGGTAGAC GGTGACATAG ACAAACAGAA GCTGATTGCA 
GCACTGGGCG CCTACACTGC TCAGAGGCTT CCTGGAGAAA ATGACCCAGA GCCACGGTAC 
CTTGTACATG GTTCCGCGAG AGCACCAAGG CCATTCTCAG CAACTGCTTT GTCTCAGAGA 
TGGCCTCCAC CTCCTGGAGA CGCCAAAGAC TCCCCGAGTA TGGATGATGA CACACTCCTG 
CAGAGTCTCC TGAAGGATTT GCAGCAGAAC TCTGAAGTGG ACCGCCTGGG CCCCCTGAAG 
GAGGAGAAAG CAGACTCAGT TGCTGGAGCC ATACAAAGTG ATCCTGCAGA GGGAAGCCAA 
GAAAGCCACG GGAGAGGGGC TGAAGGACAG CCAAGAGAGC AGACAGATGC CCCAGAGACA 
ATGCTTCAAG ATCACAGACT ATCAGAGGTG GATGACCCAG TGTACAAGGA GGTCAACCGT 
CTGAGCTTCC AGCTTGGGGA CCTCTTGAAG GACTATGGGT CTCCTCTCTT ACCTGAAGGT 
CCCCTTCTAG AAAAATCCTC CAGAGAAGAG ATTAAGAAAT CAGAGCAGCC AGAGGAGGTC 
TTGTCTTCAG AAGAGGAGAC TGCTGGGGTG GAGCATGTGA GGAGCCGGAC TTACTCCAAA 
GACCTATTTG AAAGGAAACC AAACTCAGAG CCCCAGCCCA GGAGGCTTGA GGATCAGTTC 
CAAAACCGAG CTCCAGAGTT GTGGGAGGAT GAAGAAAGCC TCAAATTGGC AGCACAGGGA 
CCCCCTAGTG GAGGCCTACA GCTGGAAGTG CAGCCTTCTG AGGAACAGCA GGGATACATC 
CTCACAGGAA ACAACCCTCT AAGTCCAGAG AAGGGGAAGC AGCTGATGGA CCAAGTTGCC 
CACATCCTCC GGGTACCTTC CAGCTTCTTT GCAGATATCA AAGTTTTGGG ACCAGCAGTG 
ACCTTCAAAG TAAGTGCCAA CATCCAAAAC ATGACAACTG CCGATGTCAT CAAGGCTGCA 
GCTGACAACA AAGACCAGCT GGAGAAGGCA ACTGGACTGA CAATCCTTCA AAGTGGAATC 
AGGCCGAAGG GAAAGCTCAA ACTCCTGCCG CATCAGGAAG AGCAAGAGGA CTCTACCAAG 
TTCATTTTGC TCACCTTCCT CTCCATTGCC TGCATCCTGG GGGTTCTCCT GGCTTCCAGC 
CTTGCCTACT GCCTCCGCCA CAACTCACAC TACAAGCTGA AGGACAAGTT GTCTGGACTA 
GGCGCTGACC CCAGTGCAGA TGCCACTGAA GCCTACCAGG AGCTATGCCG CCAGCGTATG 
GCTATTCGTC CACAGGACCG CTCTGAGGGA CCACATACAT CACGCATCAA CAGCGTCTCA 
TCCCAGTTCA GCGATGGGCC GATGCCTAGT CCTTCGGCTC GGAGCAGCAC TTCATCCTGG 
TCTGAGGAGC CTGTCCAGTC CAACATGGAC ATCTCTACTG GCCACATGAT CCTGGCCTAC 
ATGGAAGACC ATCTGAAGAA CAAGAACCGC CTGGAGAAGG AGTGGGAAGC ACTGTGCGCC 
TACCAAGCAG AGCCCAACAG CTCACTTGTG GCCCAGAGAG AGGAGAATGC ACCCAAGAAC 
CGTTCCCTGG CTGTGCTGAC CTATGACCAC TCCAGGATCC TGTTGAAGTC TCAAAACAGC 
CATGGCAGTT CCGACTACAT CAATGCCAGC CCCATTATGG ACCATGACCC ACGAAACCCC 
GCATACATTG CCACCCAAGG CCCACTTCCC GCCACGGTGG CCGACTTCTG GCAGATGGTG 
TGGGAAAGCG GCTGTGCAGT CATTGTCATG CTGACACCCC TCTCCGAGAA CGGCGTCCGG 
CAGTGCCATC ACTACTGGCC CGATGAAGGC TCCAACCTCT ACCATGTCTA CGAGGTCAAT 
CTAGTCTCTG AGCACATATG GTGCCAGGAT TTCCTGGTGA GAAGCTTTTA CCTGAAGAAC 
CTGCAGACCA ACGAGACTCG CACGGTGACC CAGTTCCACT TCCTGAGTTG GTATGACCAG 
GGAGTCCCTT CCTCCACGAG GTCACTCCTG GATTTCCGCA GAAAAGTGAA CAAATGCTAC 
CGAGGCCGCT CTTGTCCGAT CATTGTCCAT TGCAGTGACG GCGCCGGCAG GAGTGGAACC 
TACGTCCTGA TTGACATGGT TCTCAATAAG ATGGCCAAAG GTGCTAAAGA GATTGATATC 
GCAGCGACCC TGGAGCACTT GAGGGACCAG AGACCAGGCA TGGTCCAGAC AAAGGAGCAG 
TTTGAGTTTG CGCTGACAGC TGTGGCTGAG GAGGTGAATG CCATCCTGAA GGCCCTTCCC 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptprn2 ( XM_011244019.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242321.1 Mus musculus protein tyrosine phosphatase, receptor type, N polypeptide 2 (Ptprn2), transcript variant X1, mRNA.

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CloneID	OMu68553
Clone ID Related Accession (Same CDS sequence)	XM_011244019.3 , XM_011244019.2
Accession Version	XM_011244019.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	receptor-type tyrosine-protein phosphatase N2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011244019.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCCGC CGCTCCCGCT TTTGCTGCTG CTGCTGCTAC CGCCGCCGCT GCCTCGCGCT 
CTGCCCGCCC CCGCGTCTGC CCGCGGCCGG CAGCTCCCGG GGCGCCTGGG ATGCTTGTTT 
GAGGATGGCT TGTGTGGATC ACTGGAGACC TGTGTGAACG GTTTCACGTG GCAAGATGAC 
TATACCCAGC GTGTGATTGC CCAGGAGCTT GCAAACCTCC CCAAGGCCTA CCTATGGCAT 
GGGGAAGCGT CCGGCCCAGC CAGGTCCTTA CAACAGAATG CTGACAATGA AAAATGGTTC 
AGTCTGGAGA GGGAGGTGGC CCTGGCCAAG ACCCTTCGGC GCTATCTGCC CTACCTGGAG 
CTTCTGTCCC AGACCCCAAC AGCAAATGCA CACTCTAGGA TAGACCATGA GACTCGTCCA 
GCCAAGGGTG AAGACTCTTC CCCTGAGAAC ATCCTGACCT ACGTGGCCCA CACATCAGCA 
CTGACCTATC CTCCTGCAAC CCGGGCCAAG TATCCTGATA ACCTTCTGCG GCCCTTTAGC 
CGGCTCCAGC CAGATGAGCT CAGCCCCAAG GTAGACGGTG ACATAGACAA ACAGAAGCTG 
ATTGCAGCAC TGGGCGCCTA CACTGCTCAG AGGCTTCCTG GAGAAAATGA CCCAGAGCCA 
CGGTACCTTG TACATGGTTC CGCGAGAGCA CCAAGGCCAT TCTCAGCAAC TGCTTTGTCT 
CAGAGATGGC CTCCACCTCC TGGAGACGCC AAAGACTCCC CGAGTATGGA TGATGACACA 
CTCCTGCAGA GTCTCCTGAA GGATTTGCAG CAGAACTCTG AAGTGGACCG CCTGGGCCCC 
CTGAAGGAGG AGAAAGCAGA CTCAGTTGCT GGAGCCATAC AAAGTGATCC TGCAGAGGGA 
AGCCAAGAAA GCCACGGGAG AGGGGCTGAA GGACAGCCAA GAGAGCAGAC AGATGCCCCA 
GAGACAATGC TTCAAGATCA CAGACTATCA GAGGTGGATG ACCCAGTGTA CAAGGAGGTC 
AACCGTCTGA GCTTCCAGCT TGGGGACCTC TTGAAGGACT ATGGGTCTCC TCTCTTACCT 
GAAGGTCCCC TTCTAGAAAA ATCCTCCAGA GAAGAGATTA AGAAATCAGA GCAGCCAGAG 
GAGGTCTTGT CTTCAGAAGA GGAGACTGCT GGGGTGGAGC ATGTGAGGAG CCGGACTTAC 
TCCAAAGACC TATTTGAAAG GAAACCAAAC TCAGAGCCCC AGCCCAGGAG GCTTGAGGAT 
CAGTTCCAAA ACCGAGCTCC AGAGTTGTGG GAGGATGAAG AAAGCCTCAA ATTGGCAGCA 
CAGGGACCCC CTAGTGGAGG CCTACAGCTG GAAGTGCAGC CTTCTGAGGA ACAGCAGGGA 
TACATCCTCA CAGGAAACAA CCCTCTAAGT CCAGAGAAGG GGAAGCAGCT GATGGACCAA 
GTTGCCCACA TCCTCCGGGT ACCTTCCAGC TTCTTTGCAG ATATCAAAGT TTTGGGACCA 
GCAGTGACCT TCAAAGTAAG TGCCAACATC CAAAACATGA CAACTGCCGA TGTCATCAAG 
GCTGCAGCTG ACAACAAAGA CCAGCTGGAG AAGGCAACTG GACTGACAAT CCTTCAAAGT 
GGAATCAGGC CGAAGGGAAA GCTCAAACTC CTGCCGCATC AGGAAGAGCA AGAGGACTCT 
ACCAAGTTCA TTTTGCTCAC CTTCCTCTCC ATTGCCTGCA TCCTGGGGGT TCTCCTGGCT 
TCCAGCCTTG CCTACTGCCT CCGCCACAAC TCACACTACA AGCTGAAGGA CAAGTTGTCT 
GGACTAGGCG CTGACCCCAG TGCAGATGCC ACTGAAGCCT ACCAGGAGCT ATGCCGCCAG 
CGTATGGCTA TTCGTCCACA GGACCGCTCT GAGGGACCAC ATACATCACG CATCAACAGC 
GTCTCATCCC AGTTCAGCGA TGGGCCGATG CCTAGTCCTT CGGCTCGGAG CAGCACTTCA 
TCCTGGTCTG AGGAGCCTGT CCAGTCCAAC ATGGACATCT CTACTGGCCA CATGATCCTG 
GCCTACATGG AAGACCATCT GAAGAACAAG AACCGCCTGG AGAAGGAGTG GGAAGCACTG 
TGCGCCTACC AAGCAGAGCC CAACAGCTCA CTTGTGGCCC AGAGAGAGGA GAATGCACCC 
AAGAACCGTT CCCTGGCTGT GCTGACCTAT GACCACTCCA GGATCCTGTT GAAGTCTCAA 
AACAGCCATG GCAGTTCCGA CTACATCAAT GCCAGCCCCA TTATGGACCA TGACCCACGA 
AACCCCGCAT ACATTGCCAC CCAAGGCCCA CTTCCCGCCA CGGTGGCCGA CTTCTGGCAG 
ATGGTGTGGG AAAGCGGCTG TGCAGTCATT GTCATGCTGA CACCCCTCTC CGAGAACGGC 
GTCCGGCAGT GCCATCACTA CTGGCCCGAT GAAGGCTCCA ACCTCTACCA TGTCTACGAG 
GTCAATCTAG TCTCTGAGCA CATATGGTGC CAGGATTTCC TGGTGAGAAG CTTTTACCTG 
AAGAACCTGC AGACCAACGA GACTCGCACG GTGACCCAGT TCCACTTCCT GAGTTGGTAT 
GACCAGGGAG TCCCTTCCTC CACGAGGTCA CTCCTGGATT TCCGCAGAAA AGTGAACAAA 
TGCTACCGAG GCCGCTCTTG TCCGATCATT GTCCATTGCA GTGACGGCGC CGGCAGGAGT 
GGAACCTACG TCCTGATTGA CATGGTTCTC AATAAGATGG CCAAAGGTGC TAAAGAGATT 
GATATCGCAG CGACCCTGGA GCACTTGAGG GACCAGAGAC CAGGCATGGT CCAGACAAAG 
GAGCAGTTTG AGTTTGCGCT GACAGCTGTG GCTGAGGAGG TGAATGCCAT CCTGAAGGCC 
CTTCCCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242321.1
CDS	174..3065
Translation	MGPPLPLLLLLLLPPPLPRALPAPASARGRQLPGRLGCLFEDGLCGSLETCVNGFTWQDDYTQRVIAQELANLPKAYLWHGEASGPARSLQQNADNEKWFSLEREVALAKTLRRYLPYLELLSQTPTANAHSRIDHETRPAKGEDSSPENILTYVAHTSALTYPPATRAKYPDNLLRPFSRLQPDELSPKVDGDIDKQKLIAALGAYTAQRLPGENDPEPRYLVHGSARAPRPFSATALSQRWPPPPGDAKDSPSMDDDTLLQSLLKDLQQNSEVDRLGPLKEEKADSVAGAIQSDPAEGSQESHGRGAEGQPREQTDAPETMLQDHRLSEVDDPVYKEVNRLSFQLGDLLKDYGSPLLPEGPLLEKSSREEIKKSEQPEEVLSSEEETAGVEHVRSRTYSKDLFERKPNSEPQPRRLEDQFQNRAPELWEDEESLKLAAQGPPSGGLQLEVQPSEEQQGYILTGNNPLSPEKGKQLMDQVAHILRVPSSFFADIKVLGPAVTFKVSANIQNMTTADVIKAAADNKDQLEKATGLTILQSGIRPKGKLKLLPHQEEQEDSTKFILLTFLSIACILGVLLASSLAYCLRHNSHYKLKDKLSGLGADPSADATEAYQELCRQRMAIRPQDRSEGPHTSRINSVSSQFSDGPMPSPSARSSTSSWSEEPVQSNMDISTGHMILAYMEDHLKNKNRLEKEWEALCAYQAEPNSSLVAQREENAPKNRSLAVLTYDHSRILLKSQNSHGSSDYINASPIMDHDPRNPAYIATQGPLPATVADFWQMVWESGCAVIVMLTPLSENGVRQCHHYWPDEGSNLYHVYEVNLVSEHIWCQDFLVRSFYLKNLQTNETRTVTQFHFLSWYDQGVPSSTRSLLDFRRKVNKCYRGRSCPIIVHCSDGAGRSGTYVLIDMVLNKMAKGAKEIDIAATLEHLRDQRPGMVQTKEQFEFALTAVAEEVNAILKALPQ

Target ORF information:

RefSeq Version	XM_011244019.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein tyrosine phosphatase, receptor type, N polypeptide 2 (Ptprn2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244019.2

ORF Insert Sequence:

ATGGGGCCGC CGCTCCCGCT TTTGCTGCTG CTGCTGCTAC CGCCGCCGCT GCCTCGCGCT 
CTGCCCGCCC CCGCGTCTGC CCGCGGCCGG CAGCTCCCGG GGCGCCTGGG ATGCTTGTTT 
GAGGATGGCT TGTGTGGATC ACTGGAGACC TGTGTGAACG GTTTCACGTG GCAAGATGAC 
TATACCCAGC GTGTGATTGC CCAGGAGCTT GCAAACCTCC CCAAGGCCTA CCTATGGCAT 
GGGGAAGCGT CCGGCCCAGC CAGGTCCTTA CAACAGAATG CTGACAATGA AAAATGGTTC 
AGTCTGGAGA GGGAGGTGGC CCTGGCCAAG ACCCTTCGGC GCTATCTGCC CTACCTGGAG 
CTTCTGTCCC AGACCCCAAC AGCAAATGCA CACTCTAGGA TAGACCATGA GACTCGTCCA 
GCCAAGGGTG AAGACTCTTC CCCTGAGAAC ATCCTGACCT ACGTGGCCCA CACATCAGCA 
CTGACCTATC CTCCTGCAAC CCGGGCCAAG TATCCTGATA ACCTTCTGCG GCCCTTTAGC 
CGGCTCCAGC CAGATGAGCT CAGCCCCAAG GTAGACGGTG ACATAGACAA ACAGAAGCTG 
ATTGCAGCAC TGGGCGCCTA CACTGCTCAG AGGCTTCCTG GAGAAAATGA CCCAGAGCCA 
CGGTACCTTG TACATGGTTC CGCGAGAGCA CCAAGGCCAT TCTCAGCAAC TGCTTTGTCT 
CAGAGATGGC CTCCACCTCC TGGAGACGCC AAAGACTCCC CGAGTATGGA TGATGACACA 
CTCCTGCAGA GTCTCCTGAA GGATTTGCAG CAGAACTCTG AAGTGGACCG CCTGGGCCCC 
CTGAAGGAGG AGAAAGCAGA CTCAGTTGCT GGAGCCATAC AAAGTGATCC TGCAGAGGGA 
AGCCAAGAAA GCCACGGGAG AGGGGCTGAA GGACAGCCAA GAGAGCAGAC AGATGCCCCA 
GAGACAATGC TTCAAGATCA CAGACTATCA GAGGTGGATG ACCCAGTGTA CAAGGAGGTC 
AACCGTCTGA GCTTCCAGCT TGGGGACCTC TTGAAGGACT ATGGGTCTCC TCTCTTACCT 
GAAGGTCCCC TTCTAGAAAA ATCCTCCAGA GAAGAGATTA AGAAATCAGA GCAGCCAGAG 
GAGGTCTTGT CTTCAGAAGA GGAGACTGCT GGGGTGGAGC ATGTGAGGAG CCGGACTTAC 
TCCAAAGACC TATTTGAAAG GAAACCAAAC TCAGAGCCCC AGCCCAGGAG GCTTGAGGAT 
CAGTTCCAAA ACCGAGCTCC AGAGTTGTGG GAGGATGAAG AAAGCCTCAA ATTGGCAGCA 
CAGGGACCCC CTAGTGGAGG CCTACAGCTG GAAGTGCAGC CTTCTGAGGA ACAGCAGGGA 
TACATCCTCA CAGGAAACAA CCCTCTAAGT CCAGAGAAGG GGAAGCAGCT GATGGACCAA 
GTTGCCCACA TCCTCCGGGT ACCTTCCAGC TTCTTTGCAG ATATCAAAGT TTTGGGACCA 
GCAGTGACCT TCAAAGTAAG TGCCAACATC CAAAACATGA CAACTGCCGA TGTCATCAAG 
GCTGCAGCTG ACAACAAAGA CCAGCTGGAG AAGGCAACTG GACTGACAAT CCTTCAAAGT 
GGAATCAGGC CGAAGGGAAA GCTCAAACTC CTGCCGCATC AGGAAGAGCA AGAGGACTCT 
ACCAAGTTCA TTTTGCTCAC CTTCCTCTCC ATTGCCTGCA TCCTGGGGGT TCTCCTGGCT 
TCCAGCCTTG CCTACTGCCT CCGCCACAAC TCACACTACA AGCTGAAGGA CAAGTTGTCT 
GGACTAGGCG CTGACCCCAG TGCAGATGCC ACTGAAGCCT ACCAGGAGCT ATGCCGCCAG 
CGTATGGCTA TTCGTCCACA GGACCGCTCT GAGGGACCAC ATACATCACG CATCAACAGC 
GTCTCATCCC AGTTCAGCGA TGGGCCGATG CCTAGTCCTT CGGCTCGGAG CAGCACTTCA 
TCCTGGTCTG AGGAGCCTGT CCAGTCCAAC ATGGACATCT CTACTGGCCA CATGATCCTG 
GCCTACATGG AAGACCATCT GAAGAACAAG AACCGCCTGG AGAAGGAGTG GGAAGCACTG 
TGCGCCTACC AAGCAGAGCC CAACAGCTCA CTTGTGGCCC AGAGAGAGGA GAATGCACCC 
AAGAACCGTT CCCTGGCTGT GCTGACCTAT GACCACTCCA GGATCCTGTT GAAGTCTCAA 
AACAGCCATG GCAGTTCCGA CTACATCAAT GCCAGCCCCA TTATGGACCA TGACCCACGA 
AACCCCGCAT ACATTGCCAC CCAAGGCCCA CTTCCCGCCA CGGTGGCCGA CTTCTGGCAG 
ATGGTGTGGG AAAGCGGCTG TGCAGTCATT GTCATGCTGA CACCCCTCTC CGAGAACGGC 
GTCCGGCAGT GCCATCACTA CTGGCCCGAT GAAGGCTCCA ACCTCTACCA TGTCTACGAG 
GTCAATCTAG TCTCTGAGCA CATATGGTGC CAGGATTTCC TGGTGAGAAG CTTTTACCTG 
AAGAACCTGC AGACCAACGA GACTCGCACG GTGACCCAGT TCCACTTCCT GAGTTGGTAT 
GACCAGGGAG TCCCTTCCTC CACGAGGTCA CTCCTGGATT TCCGCAGAAA AGTGAACAAA 
TGCTACCGAG GCCGCTCTTG TCCGATCATT GTCCATTGCA GTGACGGCGC CGGCAGGAGT 
GGAACCTACG TCCTGATTGA CATGGTTCTC AATAAGATGG CCAAAGGTGC TAAAGAGATT 
GATATCGCAG CGACCCTGGA GCACTTGAGG GACCAGAGAC CAGGCATGGT CCAGACAAAG 
GAGCAGTTTG AGTTTGCGCT GACAGCTGTG GCTGAGGAGG TGAATGCCAT CCTGAAGGCC 
CTTCCCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pathophysiologic changes in IA-2/IA-2? null mice are secondary to alterations in the secretion of hormones and neurotransmitters.
Acta diabetologica53(1)7-12(2016 Feb)
Cai T,Notkins AL

The Ia-2? intronic miRNA, miR-153, is a negative regulator of insulin and dopamine secretion through its effect on the Cacna1c gene in mice.
Diabetologia58(10)2298-306(2015 Oct)
Xu H,Abuhatzira L,Carmona GN,Vadrevu S,Satin LS,Notkins AL

Multiple microRNAs within the 14q32 cluster target the mRNAs of major type 1 diabetes autoantigens IA-2, IA-2?, and GAD65.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology29(10)4374-83(2015 Oct)
Abuhatzira L,Xu H,Tahhan G,Boulougoura A,Sch?ffer AA,Notkins AL

The dense core vesicle protein IA-2, but not IA-2?, is required for active avoidance learning.
Neuroscience26935-42(2014 Jun)
Carmona GN,Nishimura T,Schindler CW,Panlilio LV,Notkins AL

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Ptprn2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム