Twf1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Twf1
Entrez Gene ID	19230
Full Name	twinfilin actin binding protein 1
Synonyms	A6,Ptk9,twinfilin
General protein information	Preferred Names twinfilin actin binding protein 1 Names twinfilin-1 PTK9 protein tyrosine kinase 9 actin monomer-binding protein protein A6 protein tyrosine kinase 9 twinfilin, actin-binding protein, homolog 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_008971.4	NP_032997.3	twinfilin-1
XM_006520638.1	XP_006520701.1	twinfilin-1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TWF1	NP_001229326.1
Bos taurus (cattle)	TWF1	NP_001020491.1
Anopheles gambiae (African malaria mosquito)	TWF_ANOGA	XP_310246.2
Eremothecium gossypii	AGOS_ABR105C	NP_983052.2
Magnaporthe oryzae (rice blast fungus)	MGG_07617	XP_003711539.1
Neurospora crassa	NCU07512	XP_957984.2
Xenopus tropicalis (tropical clawed frog)	twf1	NP_001011469.1
Mus musculus (house mouse)	Twf1	NP_032997.3
Rattus norvegicus (Norway rat)	Twf1	NP_001008521.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003717	XP_310245.5
	LOC700686	XP_002808023.1
Canis lupus familiaris (dog)	TWF1	XP_003433603.1
Caenorhabditis elegans (roundworm)	F38E9.5	NP_510669.1
Schizosaccharomyces pombe (fission yeast)	twf1	NP_588449.1
Gallus gallus (chicken)	TWF1	NP_001265024.1
Danio rerio (zebrafish)	twf1b	NP_001002304.1
Drosophila melanogaster (fruit fly)	twf	NP_650338.1
Saccharomyces cerevisiae (baker's yeast)	TWF1	NP_011594.3
Kluyveromyces lactis	KLLA0C15719g	XP_452902.1
Pan troglodytes (chimpanzee)	TWF1	XP_001165700.2

Gene Ontology
Bibliography

Related articles in PubMed

Twinfilin 2a regulates platelet reactivity and turnover in mice.
Stritt S, Beck S, Becker IC, V?gtle T, Hakala M, Heinze KG, Du X, Bender M, Braun A, Lappalainen P, Nieswandt B
Blood130(15)1746-1756(2017 10)

Spontaneous development of hepatocellular carcinoma with cancer stem cell properties in PR-SET7-deficient livers.
Nikolaou KC, Moulos P, Chalepakis G, Hatzis P, Oda H, Reinberg D, Talianidis I
The EMBO journal34(4)430-47(2015 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

FGF signaling promotes the expansion of A6-expressing liver cells partly via AKT-dependent activation of beta-Catenin expansion of A6(+ive) periportal cells and possibly by reprogramming of centrolobular hepatocytes.
Title: Fibroblast growth factor signaling regulates the expansion of A6-expressing hepatocytes in association with AKT-dependent ?-catenin activation.

like Twf1, mouse Twf2 is a filament barbed-end capping protein, and that two tissue-specific and biochemically distinct isoforms are generated from the Twf2 gene through alternative promoter usage
Title: Two biochemically distinct and tissue-specific twinfilin isoforms are generated from the mouse Twf2 gene by alternative promoter usage.

The crystal structure of twinfilin's C-terminal ADF-H domain in complex with an actin monomer, is presented.
Title: Structure of the actin-depolymerizing factor homology domain in complex with actin.

a novel model for how sequential interactions between actin monomers, twinfilin, capping protein, and actin filaments promote cytoskeletal dynamics
Title: Biological role and structural mechanism of twinfilin-capping protein interaction.

mammals have two twinfilin isoforms, which are differentially expressed and regulated through distinct cellular signaling pathways
Title: Mammals have two twinfilin isoforms whose subcellular localizations and tissue distributions are differentially regulated.

The following Twf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Twf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu19557	NM_008971.4 Latest version!	Mus musculus twinfilin actin binding protein 1 (Twf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu51083	XM_006520638.1 Latest version!	Mus musculus twinfilin actin binding protein 1 (Twf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Twf1 ( NM_008971.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032997.3 Mus musculus twinfilin actin binding protein 1 (Twf1), mRNA.

Price & Availability↑

CloneID	OMu19557
Clone ID Related Accession (Same CDS sequence)	NM_008971.4
Accession Version	NM_008971.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	twinfilin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC147160.2. On Nov 17, 2006 this sequence version replaced NM_008971.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC094034.1, AK077410.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGTCCCACC AGACAGGCAT CCAAGCAAGC GAAGATGTCA AAGAGATCTT TGCCCGAGCC 
AGAAACGGGA AATACAGACT TCTGAAAATA TCTATCGAAA ATGAGCAACT GGTGGTTGGC 
TCCTGTAGTC CACCTTCAGA CTCCTGGGAA CAGGACTACG ACTCCTTTGT CTTGCCCCTG 
CTGGAGGACA AGCAGCCGTG CTATGTATTA TTCAGGTTAG ACTCTCAGAA TGCCCAGGGA 
TATGAATGGA TATTCATTGC ATGGTCTCCA GATCATTCTC ATGTACGTCA GAAAATGCTG 
TATGCGGCAA CAAGAGCGAC TCTGAAGAAA GAATTTGGAG GTGGCCACAT TAAAGATGAA 
GTGTTTGGAA CAGTAAAGGA GGACGTATCA TTACATGGAT ATAAAAAGTA TTTGCTGTCC 
CAGTCTTCCC CTGCCCCGCT GACCGCCGCT GAGGAAGAAT TACGGCAGAT TAAAATCAAC 
GAGGTCCAAA CTGATGTGAG TGTGGACACA AAGCACCAGA CACTCCAAGG CGTAGCCTTT 
CCTATTTCTC GAGATGCCTT CCAGGCTTTG GAGAAGCTGA GTAAGAAGCA GCTCAACTAT 
GTGCAGTTGG AAATAGATAT AAAAAATGAA ACAATAATTT TGGCCAACAC AGAAAATACA 
GAACTGAGAG ATTTGCCAAA GAGGATTCCC AAGGATTCAG CGCGTTACCA TTTCTTTCTG 
TACAAGCATT CCCACGAAGG AGACTACTTA GAGTCCGTAG TTTTTATTTA TTCGATGCCT 
GGATACACGT GCAGCATAAG AGAGCGGATG CTGTATTCCA GCTGCAAGAG CCCTCTGCTC 
GAGATTGTAG AGCGACAGCT ACAGATGGAT GTGATCAGAA AGATTGAGAT AGACAATGGG 
GATGAGCTGA CTGCGGATTT CCTGTACGAT GAAGTCCACC CCAAGCAGCA TGCACATAAG 
CAGAGTTTTG CTAAACCGAA AGGTCCTGCG GGAAAGAGGG GGATCCGAAG ATTGATTAGG 
GGCCCAGCGG AAGCAGAAGC CACTACTGAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032997.3
CDS	49..1101
Translation	MSHQTGIQASEDVKEIFARARNGKYRLLKISIENEQLVVGSCSPPSDSWEQDYDSFVLPLLEDKQPCYVLFRLDSQNAQGYEWIFIAWSPDHSHVRQKMLYAATRATLKKEFGGGHIKDEVFGTVKEDVSLHGYKKYLLSQSSPAPLTAAEEELRQIKINEVQTDVSVDTKHQTLQGVAFPISRDAFQALEKLSKKQLNYVQLEIDIKNETIILANTENTELRDLPKRIPKDSARYHFFLYKHSHEGDYLESVVFIYSMPGYTCSIRERMLYSSCKSPLLEIVERQLQMDVIRKIEIDNGDELTADFLYDEVHPKQHAHKQSFAKPKGPAGKRGIRRLIRGPAEAEATTD

Target ORF information:

RefSeq Version	NM_008971.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus twinfilin actin binding protein 1 (Twf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008971.4

ORF Insert Sequence:

ATGTCCCACC AGACAGGCAT CCAAGCAAGC GAAGATGTCA AAGAGATCTT TGCCCGAGCC 
AGAAACGGGA AATACAGACT TCTGAAAATA TCTATCGAAA ATGAGCAACT GGTGGTTGGC 
TCCTGTAGTC CACCTTCAGA CTCCTGGGAA CAGGACTACG ACTCCTTTGT CTTGCCCCTG 
CTGGAGGACA AGCAGCCGTG CTATGTATTA TTCAGGTTAG ACTCTCAGAA TGCCCAGGGA 
TATGAATGGA TATTCATTGC ATGGTCTCCA GATCATTCTC ATGTACGTCA GAAAATGCTG 
TATGCGGCAA CAAGAGCGAC TCTGAAGAAA GAATTTGGAG GTGGCCACAT TAAAGATGAA 
GTGTTTGGAA CAGTAAAGGA GGACGTATCA TTACATGGAT ATAAAAAGTA TTTGCTGTCC 
CAGTCTTCCC CTGCCCCGCT GACCGCCGCT GAGGAAGAAT TACGGCAGAT TAAAATCAAC 
GAGGTCCAAA CTGATGTGAG TGTGGACACA AAGCACCAGA CACTCCAAGG CGTAGCCTTT 
CCTATTTCTC GAGATGCCTT CCAGGCTTTG GAGAAGCTGA GTAAGAAGCA GCTCAACTAT 
GTGCAGTTGG AAATAGATAT AAAAAATGAA ACAATAATTT TGGCCAACAC AGAAAATACA 
GAACTGAGAG ATTTGCCAAA GAGGATTCCC AAGGATTCAG CGCGTTACCA TTTCTTTCTG 
TACAAGCATT CCCACGAAGG AGACTACTTA GAGTCCGTAG TTTTTATTTA TTCGATGCCT 
GGATACACGT GCAGCATAAG AGAGCGGATG CTGTATTCCA GCTGCAAGAG CCCTCTGCTC 
GAGATTGTAG AGCGACAGCT ACAGATGGAT GTGATCAGAA AGATTGAGAT AGACAATGGG 
GATGAGCTGA CTGCGGATTT CCTGTACGAT GAAGTCCACC CCAAGCAGCA TGCACATAAG 
CAGAGTTTTG CTAAACCGAA AGGTCCTGCG GGAAAGAGGG GGATCCGAAG ATTGATTAGG 
GGCCCAGCGG AAGCAGAAGC CACTACTGAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Twf1 ( XM_006520638.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520701.1 Mus musculus twinfilin actin binding protein 1 (Twf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu51083
Clone ID Related Accession (Same CDS sequence)	XM_006520638.1
Accession Version	XM_006520638.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	twinfilin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCACC AGACAGGCAT CCAAGAGCAA CTGGTGGTTG GCTCCTGTAG TCCACCTTCA 
GACTCCTGGG AACAGGACTA CGACTCCTTT GTCTTGCCCC TGCTGGAGGA CAAGCAGCCG 
TGCTATGTAT TATTCAGGTT AGACTCTCAG AATGCCCAGG GATATGAATG GATATTCATT 
GCATGGTCTC CAGATCATTC TCATGTACGT CAGAAAATGC TGTATGCGGC AACAAGAGCG 
ACTCTGAAGA AAGAATTTGG AGGTGGCCAC ATTAAAGATG AAGTGTTTGG AACAGTAAAG 
GAGGACGTAT CATTACATGG ATATAAAAAG TATTTGCTGT CCCAGTCTTC CCCTGCCCCG 
CTGACCGCCG CTGAGGAAGA ATTACGGCAG ATTAAAATCA ACGAGGTCCA AACTGATGTG 
AGTGTGGACA CAAAGCACCA GACACTCCAA GGCGTAGCCT TTCCTATTTC TCGAGATGCC 
TTCCAGGCTT TGGAGAAGCT GAGTAAGAAG CAGCTCAACT ATGTGCAGTT GGAAATAGAT 
ATAAAAAATG AAACAATAAT TTTGGCCAAC ACAGAAAATA CAGAACTGAG AGATTTGCCA 
AAGAGGATTC CCAAGGATTC AGCGCGTTAC CATTTCTTTC TGTACAAGCA TTCCCACGAA 
GGAGACTACT TAGAGTCCGT AGTTTTTATT TATTCGATGC CTGGATACAC GTGCAGCATA 
AGAGAGCGGA TGCTGTATTC CAGCTGCAAG AGCCCTCTGC TCGAGATTGT AGAGCGACAG 
CTACAGATGG ATGTGATCAG AAAGATTGAG ATAGACAATG GGGATGAGCT GACTGCGGAT 
TTCCTGTACG ATGAAGTCCA CCCCAAGCAG CATGCACATA AGCAGAGTTT TGCTAAACCG 
AAAGGTCCTG CGGGAAAGAG GGGGATCCGA AGATTGATTA GGGGCCCAGC GGAAGCAGAA 
GCCACTACTG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520701.1
CDS	109..1083
Translation	MSHQTGIQEQLVVGSCSPPSDSWEQDYDSFVLPLLEDKQPCYVLFRLDSQNAQGYEWIFIAWSPDHSHVRQKMLYAATRATLKKEFGGGHIKDEVFGTVKEDVSLHGYKKYLLSQSSPAPLTAAEEELRQIKINEVQTDVSVDTKHQTLQGVAFPISRDAFQALEKLSKKQLNYVQLEIDIKNETIILANTENTELRDLPKRIPKDSARYHFFLYKHSHEGDYLESVVFIYSMPGYTCSIRERMLYSSCKSPLLEIVERQLQMDVIRKIEIDNGDELTADFLYDEVHPKQHAHKQSFAKPKGPAGKRGIRRLIRGPAEAEATTD

Target ORF information:

RefSeq Version	XM_006520638.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus twinfilin actin binding protein 1 (Twf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520638.1

ORF Insert Sequence:

ATGTCCCACC AGACAGGCAT CCAAGAGCAA CTGGTGGTTG GCTCCTGTAG TCCACCTTCA 
GACTCCTGGG AACAGGACTA CGACTCCTTT GTCTTGCCCC TGCTGGAGGA CAAGCAGCCG 
TGCTATGTAT TATTCAGGTT AGACTCTCAG AATGCCCAGG GATATGAATG GATATTCATT 
GCATGGTCTC CAGATCATTC TCATGTACGT CAGAAAATGC TGTATGCGGC AACAAGAGCG 
ACTCTGAAGA AAGAATTTGG AGGTGGCCAC ATTAAAGATG AAGTGTTTGG AACAGTAAAG 
GAGGACGTAT CATTACATGG ATATAAAAAG TATTTGCTGT CCCAGTCTTC CCCTGCCCCG 
CTGACCGCCG CTGAGGAAGA ATTACGGCAG ATTAAAATCA ACGAGGTCCA AACTGATGTG 
AGTGTGGACA CAAAGCACCA GACACTCCAA GGCGTAGCCT TTCCTATTTC TCGAGATGCC 
TTCCAGGCTT TGGAGAAGCT GAGTAAGAAG CAGCTCAACT ATGTGCAGTT GGAAATAGAT 
ATAAAAAATG AAACAATAAT TTTGGCCAAC ACAGAAAATA CAGAACTGAG AGATTTGCCA 
AAGAGGATTC CCAAGGATTC AGCGCGTTAC CATTTCTTTC TGTACAAGCA TTCCCACGAA 
GGAGACTACT TAGAGTCCGT AGTTTTTATT TATTCGATGC CTGGATACAC GTGCAGCATA 
AGAGAGCGGA TGCTGTATTC CAGCTGCAAG AGCCCTCTGC TCGAGATTGT AGAGCGACAG 
CTACAGATGG ATGTGATCAG AAAGATTGAG ATAGACAATG GGGATGAGCT GACTGCGGAT 
TTCCTGTACG ATGAAGTCCA CCCCAAGCAG CATGCACATA AGCAGAGTTT TGCTAAACCG 
AAAGGTCCTG CGGGAAAGAG GGGGATCCGA AGATTGATTA GGGGCCCAGC GGAAGCAGAA 
GCCACTACTG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Twinfilin 2a regulates platelet reactivity and turnover in mice.
Blood130(15)1746-1756(2017 10)
Stritt S,Beck S,Becker IC,V?gtle T,Hakala M,Heinze KG,Du X,Bender M,Braun A,Lappalainen P,Nieswandt B

Spontaneous development of hepatocellular carcinoma with cancer stem cell properties in PR-SET7-deficient livers.
The EMBO journal34(4)430-47(2015 Feb)
Nikolaou KC,Moulos P,Chalepakis G,Hatzis P,Oda H,Reinberg D,Talianidis I