Pole2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pole2
Entrez Gene ID	18974
Full Name	polymerase (DNA directed), epsilon 2 (p59 subunit)
General protein information	Preferred Names polymerase (DNA directed), epsilon 2 (p59 subunit) Names DNA polymerase epsilon subunit 2 DNA polymerase II subunit 2 DNA polymerase epsilon small subunit DNA polymerase epsilon subunit B
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 C2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006515598.3	XP_006515661.1	DNA polymerase epsilon subunit 2 isoform X2
NM_011133.2	NP_035263.1	DNA polymerase epsilon subunit 2
XM_006515598.4	XP_006515661.1	DNA polymerase epsilon subunit 2 isoform X2
XM_011244018.1	XP_011242320.1	DNA polymerase epsilon subunit 2 isoform X1
XM_011244018.2	XP_011242320.1	DNA polymerase epsilon subunit 2 isoform X1
XM_006515599.1	XP_006515662.1	DNA polymerase epsilon subunit 2 isoform X3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	POLE2	NP_001098853.1
Rattus norvegicus (Norway rat)	Pole2	NP_001162579.1
Gallus gallus (chicken)	POLE2	NP_001006493.1
Danio rerio (zebrafish)	pole2	NP_775353.1
Xenopus tropicalis (tropical clawed frog)	pole2	NP_001025641.1
Pan troglodytes (chimpanzee)	POLE2	XP_509931.2
Canis lupus familiaris (dog)	POLE2	XP_851216.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004614	XP_315206.3
Arabidopsis thaliana (thale cress)	DPB2	NP_680197.2
Caenorhabditis elegans (roundworm)	pole-2	NP_501489.2
	POLE2	XP_001095940.2
Drosophila melanogaster (fruit fly)	Pole2	NP_647995.1
Oryza sativa (rice)	Os05g0160800	NP_001054723.1
Homo sapiens (human)	POLE2	NP_002683.2
Mus musculus (house mouse)	Pole2	NP_035263.1

Gene Ontology
Bibliography

Related articles in PubMed

Role of FEN1 S187 phosphorylation in counteracting oxygen-induced stress and regulating postnatal heart development.
Zhou L, Dai H, Wu J, Zhou M, Yuan H, Du J, Yang L, Wu X, Xu H, Hua Y, Xu J, Zheng L, Shen B
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(1)132-147(2017 01)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

The second largest subunit of mouse DNA polymerase epsilon, DPE2, interacts with SAP18 and recruits the Sin3 co-repressor protein to DNA.
Wada M, Miyazawa H, Wang RS, Mizuno T, Sato A, Asashima M, Hanaoka F
Journal of biochemistry131(3)307-11(2002 Mar)

The small subunits of human and mouse DNA polymerase epsilon are homologous to the second largest subunit of the yeast Saccharomyces cerevisiae DNA polymerase epsilon.
Jokela M, M?kiniemi M, Lehtonen S, Szpirer C, Hellman U, Syv?oja JE
Nucleic acids research26(3)730-4(1998 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The second largest subunit of mouse DNA polymerase epsilon was found to interact with SAP18. The interaction induced repression of transcription.
Title: The second largest subunit of mouse DNA polymerase epsilon, DPE2, interacts with SAP18 and recruits the Sin3 co-repressor protein to DNA.

The following Pole2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pole2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu07529	NM_011133.2 Latest version!	Mus musculus polymerase (DNA directed), epsilon 2 (p59 subunit) (Pole2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu47669	XM_006515599.1 Latest version!	Mus musculus polymerase (DNA directed), epsilon 2 (p59 subunit) (Pole2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu47668	XM_006515598.4 Latest version!	Mus musculus polymerase (DNA directed), epsilon 2 (p59 subunit) (Pole2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu68465	XM_011244018.2 Latest version!	Mus musculus polymerase (DNA directed), epsilon 2 (p59 subunit) (Pole2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu47668	XM_006515598.3	Mus musculus polymerase (DNA directed), epsilon 2 (p59 subunit) (Pole2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu68465	XM_011244018.1	Mus musculus polymerase (DNA directed), epsilon 2 (p59 subunit) (Pole2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pole2 ( NM_011133.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035263.1 Mus musculus polymerase (DNA directed), epsilon 2 (p59 subunit) (Pole2), mRNA.

Price & Availability↑

CloneID	OMu07529
Clone ID Related Accession (Same CDS sequence)	NM_011133.2
Accession Version	NM_011133.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-12
Organism	Mus musculus(house mouse)
Product	DNA polymerase epsilon subunit 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK077592.1 and AF036898.1. On Apr 5, 2007 this sequence version replaced NM_011133.1. ##Evidence-Data-START## Transcript exon combination :: AK077592.1, BC063772.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCGCCGG AGCGGCTGCG GAGCCGCACG GTCTCAGCTT TCAAGCTGCG GGGCTTGCTG 
CTCAGGAGTG AAGCCACCAA GTATCTGACT GAAGCTCTCC AGTCTGTCAC TGAATTGGAG 
CTAGAGGAGA ACCTAGGAAA GATCATCGAT GCAGTTGAAA AGCAGCCCTT GTCCTCGAAC 
ATGATCGAAC GATCTGTCGT GGAGGCAGCT GTGCAAGAGT GCAGTCAGTC TATGGATGAG 
ACTATAGAGC ACGTTTTCAA TATCATTGGA GCTTTTGATA TTCCACGTTT TATATATAAT 
TCAGAAAGGA AAAAATTTCT TCCTCTATCC ATGACTAATC ATGCTGCACC AACCTTATTT 
GGAACAGCAA GAGACAAAGC CGAGCTGTAT CTTGAACGCT ATACCATTTT ACACCAGAGG 
ACACACAGAC ATGAATTGTT CACTCCTCCG GTGATAGGCT CACACCTTGA AGAAAGTGGG 
AGCAAGTTCC AGCTTAAAAC TATAGAAACC TTACTGGGTA GCACAAGCAA AGTTGGTGAC 
GTGATCGTCC TGGGGATGAT AACCCAGCTG AAAGAGGGGA AGTTTTTCCT GGAAGATCCT 
ACTGGAACAG TACAACTGGA CCTTAGTAAA GCTCAGTTCC ACAGTGGCTT ATACACCGAG 
GCCTGCTTCG TCCTGGCAGA AGGTTGGTTT GAAGATCAAG TGTTTCACGC CAATGCCTTT 
GGATTTCCTC CCACTGAGCC CTCCAGCACC ACAAGGGCGT ACTATGGAAA TATTAATTTT 
TTTGGAGGGC CTTCTAATGC ATCAGTGAAG ACGTATACAA AGCTGAGACA GCTGGAAGAT 
GAGAACAAGG ACGCCATGTT TGTGATTGTG TCTGATGTTT GGTTGGACCA AGTGCAAGTG 
TTGGAGAAGT TTCATGTAAT GTTTTCTGGT TACTCGCCAG CACCTCCAAC CTGCTTTATT 
CTGTGTGGTA ATTTTTCATC TGCACCATAT GGAAAAAATC AAGTCCAGGC TTTGAAAGAT 
TCCCTAAAAT CTTTGGCAGA CATAATTTGT GAATATCCAA GTATCCACCA AAGTAGCCGT 
TTTGTGTTTG TGCCTGGCCC AGAGGATCCT GGATTTGGTT CCATTTTACC AAGGCCGCCA 
CTCGCTGAAA GCATCACACA GGAATTCAGA CAAAGGGTTC CGTTTTCAGT TTTCACGACT 
AATCCTTGCA GAATTCAGTA TTGTACACAA GAAATAATTA TCTTCCGTGA AGACTTAGTG 
AATAAAATGT GTAGAAACTG TGTCCGTTTT CCTAGCAGCA GTTTGGATAT TCCTACTCAC 
TTCGTAAAGA CCATCTTATC TCAGGGACAT CTGGCTCCTC TCCCTCTTTA CGTATGCCCC 
GTCCACTGGG CATATGATTA CACGTTGAGA GTGTACCCGG TGCCCGACCT GCTGGTCATT 
GCAGACAAGT ACGACCCGTT CACCGTGACC AACACCGATT GCCTCTGCAT AAACCCTGGC 
TCCTTTCCAA GAAGTGGATT TGCATTCAAA GTTTTTTACC CTTCCAGTAA AACAGTAGAA 
GACAGCAAAC TTCAGGGCTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035263.1
CDS	27..1610
Translation	MAPERLRSRTVSAFKLRGLLLRSEATKYLTEALQSVTELELEENLGKIIDAVEKQPLSSNMIERSVVEAAVQECSQSMDETIEHVFNIIGAFDIPRFIYNSERKKFLPLSMTNHAAPTLFGTARDKAELYLERYTILHQRTHRHELFTPPVIGSHLEESGSKFQLKTIETLLGSTSKVGDVIVLGMITQLKEGKFFLEDPTGTVQLDLSKAQFHSGLYTEACFVLAEGWFEDQVFHANAFGFPPTEPSSTTRAYYGNINFFGGPSNASVKTYTKLRQLEDENKDAMFVIVSDVWLDQVQVLEKFHVMFSGYSPAPPTCFILCGNFSSAPYGKNQVQALKDSLKSLADIICEYPSIHQSSRFVFVPGPEDPGFGSILPRPPLAESITQEFRQRVPFSVFTTNPCRIQYCTQEIIIFREDLVNKMCRNCVRFPSSSLDIPTHFVKTILSQGHLAPLPLYVCPVHWAYDYTLRVYPVPDLLVIADKYDPFTVTNTDCLCINPGSFPRSGFAFKVFYPSSKTVEDSKLQGF

Target ORF information:

RefSeq Version	NM_011133.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA directed), epsilon 2 (p59 subunit) (Pole2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011133.2

ORF Insert Sequence:

ATGGCGCCGG AGCGGCTGCG GAGCCGCACG GTCTCAGCTT TCAAGCTGCG GGGCTTGCTG 
CTCAGGAGTG AAGCCACCAA GTATCTGACT GAAGCTCTCC AGTCTGTCAC TGAATTGGAG 
CTAGAGGAGA ACCTAGGAAA GATCATCGAT GCAGTTGAAA AGCAGCCCTT GTCCTCGAAC 
ATGATCGAAC GATCTGTCGT GGAGGCAGCT GTGCAAGAGT GCAGTCAGTC TATGGATGAG 
ACTATAGAGC ACGTTTTCAA TATCATTGGA GCTTTTGATA TTCCACGTTT TATATATAAT 
TCAGAAAGGA AAAAATTTCT TCCTCTATCC ATGACTAATC ATGCTGCACC AACCTTATTT 
GGAACAGCAA GAGACAAAGC CGAGCTGTAT CTTGAACGCT ATACCATTTT ACACCAGAGG 
ACACACAGAC ATGAATTGTT CACTCCTCCG GTGATAGGCT CACACCTTGA AGAAAGTGGG 
AGCAAGTTCC AGCTTAAAAC TATAGAAACC TTACTGGGTA GCACAAGCAA AGTTGGTGAC 
GTGATCGTCC TGGGGATGAT AACCCAGCTG AAAGAGGGGA AGTTTTTCCT GGAAGATCCT 
ACTGGAACAG TACAACTGGA CCTTAGTAAA GCTCAGTTCC ACAGTGGCTT ATACACCGAG 
GCCTGCTTCG TCCTGGCAGA AGGTTGGTTT GAAGATCAAG TGTTTCACGC CAATGCCTTT 
GGATTTCCTC CCACTGAGCC CTCCAGCACC ACAAGGGCGT ACTATGGAAA TATTAATTTT 
TTTGGAGGGC CTTCTAATGC ATCAGTGAAG ACGTATACAA AGCTGAGACA GCTGGAAGAT 
GAGAACAAGG ACGCCATGTT TGTGATTGTG TCTGATGTTT GGTTGGACCA AGTGCAAGTG 
TTGGAGAAGT TTCATGTAAT GTTTTCTGGT TACTCGCCAG CACCTCCAAC CTGCTTTATT 
CTGTGTGGTA ATTTTTCATC TGCACCATAT GGAAAAAATC AAGTCCAGGC TTTGAAAGAT 
TCCCTAAAAT CTTTGGCAGA CATAATTTGT GAATATCCAA GTATCCACCA AAGTAGCCGT 
TTTGTGTTTG TGCCTGGCCC AGAGGATCCT GGATTTGGTT CCATTTTACC AAGGCCGCCA 
CTCGCTGAAA GCATCACACA GGAATTCAGA CAAAGGGTTC CGTTTTCAGT TTTCACGACT 
AATCCTTGCA GAATTCAGTA TTGTACACAA GAAATAATTA TCTTCCGTGA AGACTTAGTG 
AATAAAATGT GTAGAAACTG TGTCCGTTTT CCTAGCAGCA GTTTGGATAT TCCTACTCAC 
TTCGTAAAGA CCATCTTATC TCAGGGACAT CTGGCTCCTC TCCCTCTTTA CGTATGCCCC 
GTCCACTGGG CATATGATTA CACGTTGAGA GTGTACCCGG TGCCCGACCT GCTGGTCATT 
GCAGACAAGT ACGACCCGTT CACCGTGACC AACACCGATT GCCTCTGCAT AAACCCTGGC 
TCCTTTCCAA GAAGTGGATT TGCATTCAAA GTTTTTTACC CTTCCAGTAA AACAGTAGAA 
GACAGCAAAC TTCAGGGCTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pole2 ( XM_006515598.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515661.1 Mus musculus polymerase (DNA directed), epsilon 2 (p59 subunit) (Pole2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu47668
Clone ID Related Accession (Same CDS sequence)	XM_006515598.3 , XM_006515598.4
Accession Version	XM_006515598.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	DNA polymerase epsilon subunit 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515598.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCGG AGCGGCTGCG GAGCCGCACG GTCTCAGCTT TCAAGCTGCG GGGCTTGCTG 
CTCAGGAGTG AAGCCACCAA GTATCTGACT GAAGCTCTCC AGTCTGTCAC TGAATTGGAG 
CTAGAGGAGA ACCTAGGAAA GATCATCGAT GCAGTTGAAA AGCAGCCCTT GTCCTCGAAC 
ATGATCGAAC GATCTGTCGT GGAGGCAGCT GTGCAAGAGT GCAGTCAGTC TATGGATGAG 
ACTATAGAGC ACGTTTTCAA TATCATTGGA GCTTTTGATA TTCCACGTTT TATATATAAT 
TCAGAAAGGA AAAAATTTCT TCCTCTATCC ATGACTAATC ATGCTGCACC AACCTTATTT 
GGAACAGCAA GAGACAAAGC CGAGCTGTAT CTTGAACGCT ATACCATTTT ACACCAGAGG 
ACACACAGAC ATGAATTGTT CACTCCTCCG GTGATAGGCT CACACCTTGA AGAAAGTGGG 
AGCAAGTTCC AGCTTAAAAC TATAGAAACC TTACTGGGTA GCACAAGCAA AGTTGGTGAC 
GTGATCGTCC TGGGGATGAT AACCCAGCTG AAAGAGGGGA AGTTTTTCCT GGAAGATCCT 
ACTGGAACAG TACAACTGGA CCTTAGTAAA GCTCAGTTCC ACAGTGGCTT ATACACCGAG 
GCCTGCTTCG TCCTGGCAGA AGGTTGGTTT GAAGATCAAG TGTTTCACGC CAATGCCTTT 
GGATTTCCTC CCACTGAGCC CTCCAGCACC ACAAGGGCGT ACTATGGAAA TATTAATTTT 
TTTGGAGGGC CTTCTAATGC ATCAGTGAAG ACGTATACAA AGCTGAGACA GCTGGAAGAT 
GAGAACAAGG ACGCCATGTT TGTGATTGTG TCTGATGTTT GGTTGGACCA AGTGCAAGTG 
TTGGAGAAGT TTCATGTAAT GTTTTCTGGT TACTCGCCAG CACCTCCAAC CTGCTTTATT 
CTGTGTGGTA ATTTTTCATC TGCACCATAT GGAAAAAATC AAGTCCAGGC TTTGAAAGAT 
TCCCTAAAAT CTTTGGCAGA CATAATTTGT GAATATCCAA GTATCCACCA AAGTAGCCGT 
TTTGTGTTTG TGCCTGGCCC AGAGGATCCT GGATTTGGTT CCATTTTACC AAGGCCGCCA 
CTCGCTGAAA GCATCACACA GGAATTCAGA CAAAGGGTTC CGTTTTCAGT TTTCACGACT 
AATCCTTGCA GTGGTATCTC CAGCTTCCTC AGGAATCCCA TCCTTGTGGG TCCAGATGTC 
CAGTGTCCTT TCTCTCCGGA TGCCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515661.1
CDS	84..1373
Translation	MAPERLRSRTVSAFKLRGLLLRSEATKYLTEALQSVTELELEENLGKIIDAVEKQPLSSNMIERSVVEAAVQECSQSMDETIEHVFNIIGAFDIPRFIYNSERKKFLPLSMTNHAAPTLFGTARDKAELYLERYTILHQRTHRHELFTPPVIGSHLEESGSKFQLKTIETLLGSTSKVGDVIVLGMITQLKEGKFFLEDPTGTVQLDLSKAQFHSGLYTEACFVLAEGWFEDQVFHANAFGFPPTEPSSTTRAYYGNINFFGGPSNASVKTYTKLRQLEDENKDAMFVIVSDVWLDQVQVLEKFHVMFSGYSPAPPTCFILCGNFSSAPYGKNQVQALKDSLKSLADIICEYPSIHQSSRFVFVPGPEDPGFGSILPRPPLAESITQEFRQRVPFSVFTTNPCSGISSFLRNPILVGPDVQCPFSPDAL

Target ORF information:

RefSeq Version	XM_006515598.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA directed), epsilon 2 (p59 subunit) (Pole2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515598.3

ORF Insert Sequence:

ATGGCGCCGG AGCGGCTGCG GAGCCGCACG GTCTCAGCTT TCAAGCTGCG GGGCTTGCTG 
CTCAGGAGTG AAGCCACCAA GTATCTGACT GAAGCTCTCC AGTCTGTCAC TGAATTGGAG 
CTAGAGGAGA ACCTAGGAAA GATCATCGAT GCAGTTGAAA AGCAGCCCTT GTCCTCGAAC 
ATGATCGAAC GATCTGTCGT GGAGGCAGCT GTGCAAGAGT GCAGTCAGTC TATGGATGAG 
ACTATAGAGC ACGTTTTCAA TATCATTGGA GCTTTTGATA TTCCACGTTT TATATATAAT 
TCAGAAAGGA AAAAATTTCT TCCTCTATCC ATGACTAATC ATGCTGCACC AACCTTATTT 
GGAACAGCAA GAGACAAAGC CGAGCTGTAT CTTGAACGCT ATACCATTTT ACACCAGAGG 
ACACACAGAC ATGAATTGTT CACTCCTCCG GTGATAGGCT CACACCTTGA AGAAAGTGGG 
AGCAAGTTCC AGCTTAAAAC TATAGAAACC TTACTGGGTA GCACAAGCAA AGTTGGTGAC 
GTGATCGTCC TGGGGATGAT AACCCAGCTG AAAGAGGGGA AGTTTTTCCT GGAAGATCCT 
ACTGGAACAG TACAACTGGA CCTTAGTAAA GCTCAGTTCC ACAGTGGCTT ATACACCGAG 
GCCTGCTTCG TCCTGGCAGA AGGTTGGTTT GAAGATCAAG TGTTTCACGC CAATGCCTTT 
GGATTTCCTC CCACTGAGCC CTCCAGCACC ACAAGGGCGT ACTATGGAAA TATTAATTTT 
TTTGGAGGGC CTTCTAATGC ATCAGTGAAG ACGTATACAA AGCTGAGACA GCTGGAAGAT 
GAGAACAAGG ACGCCATGTT TGTGATTGTG TCTGATGTTT GGTTGGACCA AGTGCAAGTG 
TTGGAGAAGT TTCATGTAAT GTTTTCTGGT TACTCGCCAG CACCTCCAAC CTGCTTTATT 
CTGTGTGGTA ATTTTTCATC TGCACCATAT GGAAAAAATC AAGTCCAGGC TTTGAAAGAT 
TCCCTAAAAT CTTTGGCAGA CATAATTTGT GAATATCCAA GTATCCACCA AAGTAGCCGT 
TTTGTGTTTG TGCCTGGCCC AGAGGATCCT GGATTTGGTT CCATTTTACC AAGGCCGCCA 
CTCGCTGAAA GCATCACACA GGAATTCAGA CAAAGGGTTC CGTTTTCAGT TTTCACGACT 
AATCCTTGCA GTGGTATCTC CAGCTTCCTC AGGAATCCCA TCCTTGTGGG TCCAGATGTC 
CAGTGTCCTT TCTCTCCGGA TGCCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pole2 ( XM_006515599.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515662.1 Mus musculus polymerase (DNA directed), epsilon 2 (p59 subunit) (Pole2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu47669
Clone ID Related Accession (Same CDS sequence)	XM_006515599.1
Accession Version	XM_006515599.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	DNA polymerase epsilon subunit 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTGG ATTTCCTCCC ACTGAGCCCT CCAGCACCAC AAGGGCCTTC TAATGCATCA 
GTGAAGACGT ATACAAAGCT GAGACAGCTG GAAGATGAGA ACAAGGACGC CATGTTTGTG 
ATTGTGTCTG ATGTTTGGTT GGACCAAGTG CAAGTGTTGG AGAAGTTTCA TGTAATGTTT 
TCTGGTTACT CGCCAGCACC TCCAACCTGC TTTATTCTGT GTGGTAATTT TTCATCTGCA 
CCATATGGAA AAAATCAAGT CCAGGCTTTG AAAGATTCCC TAAAATCTTT GGCAGACATA 
ATTTGTGAAT ATCCAAGTAT CCACCAAAGT AGCCGTTTTG TGTTTGTGCC TGGCCCAGAG 
GATCCTGGAT TTGGTTCCAT TTTACCAAGG CCGCCACTCG CTGAAAGCAT CACACAGGAA 
TTCAGACAAA GGGTTCCGTT TTCAGTTTTC ACGACTAATC CTTGCAGAAT TCAGTATTGT 
ACACAAGAAA TAATTATCTT CCGTGAAGAC TTAGTGAATA AAATGTGTAG AAACTGTGTC 
CGTTTTCCTA GCAGCAGTTT GGATATTCCT ACTCACTTCG TAAAGACCAT CTTATCTCAG 
GGACATCTGG CTCCTCTCCC TCTTTACGTA TGCCCCGTCC ACTGGGCATA TGATTACACG 
TTGAGAGTGT ACCCGGTGCC CGACCTGCTG GTCATTGCAG ACAAGTACGA CCCGTTCACC 
GTGACCAACA CCGATTGCCT CTGCATAAAC CCTGGCTCCT TTCCAAGAAG TGGATTTGCA 
TTCAAAGTTT TTTACCCTTC CAGTAAAACA GTAGAAGACA GCAAACTTCA GGGCTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515662.1
CDS	281..1120
Translation	MPLDFLPLSPPAPQGPSNASVKTYTKLRQLEDENKDAMFVIVSDVWLDQVQVLEKFHVMFSGYSPAPPTCFILCGNFSSAPYGKNQVQALKDSLKSLADIICEYPSIHQSSRFVFVPGPEDPGFGSILPRPPLAESITQEFRQRVPFSVFTTNPCRIQYCTQEIIIFREDLVNKMCRNCVRFPSSSLDIPTHFVKTILSQGHLAPLPLYVCPVHWAYDYTLRVYPVPDLLVIADKYDPFTVTNTDCLCINPGSFPRSGFAFKVFYPSSKTVEDSKLQGF

Target ORF information:

RefSeq Version	XM_006515599.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA directed), epsilon 2 (p59 subunit) (Pole2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515599.1

ORF Insert Sequence:

ATGCCTTTGG ATTTCCTCCC ACTGAGCCCT CCAGCACCAC AAGGGCCTTC TAATGCATCA 
GTGAAGACGT ATACAAAGCT GAGACAGCTG GAAGATGAGA ACAAGGACGC CATGTTTGTG 
ATTGTGTCTG ATGTTTGGTT GGACCAAGTG CAAGTGTTGG AGAAGTTTCA TGTAATGTTT 
TCTGGTTACT CGCCAGCACC TCCAACCTGC TTTATTCTGT GTGGTAATTT TTCATCTGCA 
CCATATGGAA AAAATCAAGT CCAGGCTTTG AAAGATTCCC TAAAATCTTT GGCAGACATA 
ATTTGTGAAT ATCCAAGTAT CCACCAAAGT AGCCGTTTTG TGTTTGTGCC TGGCCCAGAG 
GATCCTGGAT TTGGTTCCAT TTTACCAAGG CCGCCACTCG CTGAAAGCAT CACACAGGAA 
TTCAGACAAA GGGTTCCGTT TTCAGTTTTC ACGACTAATC CTTGCAGAAT TCAGTATTGT 
ACACAAGAAA TAATTATCTT CCGTGAAGAC TTAGTGAATA AAATGTGTAG AAACTGTGTC 
CGTTTTCCTA GCAGCAGTTT GGATATTCCT ACTCACTTCG TAAAGACCAT CTTATCTCAG 
GGACATCTGG CTCCTCTCCC TCTTTACGTA TGCCCCGTCC ACTGGGCATA TGATTACACG 
TTGAGAGTGT ACCCGGTGCC CGACCTGCTG GTCATTGCAG ACAAGTACGA CCCGTTCACC 
GTGACCAACA CCGATTGCCT CTGCATAAAC CCTGGCTCCT TTCCAAGAAG TGGATTTGCA 
TTCAAAGTTT TTTACCCTTC CAGTAAAACA GTAGAAGACA GCAAACTTCA GGGCTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pole2 ( XM_011244018.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242320.1 Mus musculus polymerase (DNA directed), epsilon 2 (p59 subunit) (Pole2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu68465
Clone ID Related Accession (Same CDS sequence)	XM_011244018.1 , XM_011244018.2
Accession Version	XM_011244018.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	DNA polymerase epsilon subunit 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGAAC GATCTGTCGT GGAGGCAGCT GTGCAAGAGT GCAGTCAGTC TATGGATGAG 
ACTATAGAGC ACGTTTTCAA TATCATTGGA GCTTTTGATA TTCCACGTTT TATATATAAT 
TCAGAAAGGA AAAAATTTCT TCCTCTATCC ATGACTAATC ATGCTGCACC AACCTTATTT 
GGAACAGCAA GAGACAAAGC CGAGCTGTAT CTTGAACGCT ATACCATTTT ACACCAGAGG 
ACACACAGAC ATGAATTGTT CACTCCTCCG GTGATAGGCT CACACCTTGA AGAAAGTGGG 
AGCAAGTTCC AGCTTAAAAC TATAGAAACC TTACTGGGTA GCACAAGCAA AGTTGGTGAC 
GTGATCGTCC TGGGGATGAT AACCCAGCTG AAAGAGGGGA AGTTTTTCCT GGAAGATCCT 
ACTGGAACAG TACAACTGGA CCTTAGTAAA GCTCAGTTCC ACAGTGGCTT ATACACCGAG 
GCCTGCTTCG TCCTGGCAGA AGGTTGGTTT GAAGATCAAG TGTTTCACGC CAATGCCTTT 
GGATTTCCTC CCACTGAGCC CTCCAGCACC ACAAGGGCGT ACTATGGAAA TATTAATTTT 
TTTGGAGGGC CTTCTAATGC ATCAGTGAAG ACGTATACAA AGCTGAGACA GCTGGAAGAT 
GAGAACAAGG ACGCCATGTT TGTGATTGTG TCTGATGTTT GGTTGGACCA AGTGCAAGTG 
TTGGAGAAGT TTCATGTAAT GTTTTCTGGT TACTCGCCAG CACCTCCAAC CTGCTTTATT 
CTGTGTGGTA ATTTTTCATC TGCACCATAT GGAAAAAATC AAGTCCAGGC TTTGAAAGAT 
TCCCTAAAAT CTTTGGCAGA CATAATTTGT GAATATCCAA GTATCCACCA AAGTAGCCGT 
TTTGTGTTTG TGCCTGGCCC AGAGGATCCT GGATTTGGTT CCATTTTACC AAGGCCGCCA 
CTCGCTGAAA GCATCACACA GGAATTCAGA CAAAGGGTTC CGTTTTCAGT TTTCACGACT 
AATCCTTGCA GAATTCAGTA TTGTACACAA GAAATAATTA TCTTCCGTGA AGACTTAGTG 
AATAAAATGT GTAGAAACTG TGTCCGTTTT CCTAGCAGCA GTTTGGATAT TCCTACTCAC 
TTCGTAAAGA CCATCTTATC TCAGGGACAT CTGGCTCCTC TCCCTCTTTA CGTATGCCCC 
GTCCACTGGG CATATGATTA CACGTTGAGA GTGTACCCGG TGCCCGACCT GCTGGTCATT 
GCAGACAAGT ACGACCCGTT CACCGTGACC AACACCGATT GCCTCTGCAT AAACCCTGGC 
TCCTTTCCAA GAAGTGGATT TGCATTCAAA GTTTTTTACC CTTCCAGTAA AACAGTAGAA 
GACAGCAAAC TTCAGGGCTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242320.1
CDS	201..1604
Translation	MIERSVVEAAVQECSQSMDETIEHVFNIIGAFDIPRFIYNSERKKFLPLSMTNHAAPTLFGTARDKAELYLERYTILHQRTHRHELFTPPVIGSHLEESGSKFQLKTIETLLGSTSKVGDVIVLGMITQLKEGKFFLEDPTGTVQLDLSKAQFHSGLYTEACFVLAEGWFEDQVFHANAFGFPPTEPSSTTRAYYGNINFFGGPSNASVKTYTKLRQLEDENKDAMFVIVSDVWLDQVQVLEKFHVMFSGYSPAPPTCFILCGNFSSAPYGKNQVQALKDSLKSLADIICEYPSIHQSSRFVFVPGPEDPGFGSILPRPPLAESITQEFRQRVPFSVFTTNPCRIQYCTQEIIIFREDLVNKMCRNCVRFPSSSLDIPTHFVKTILSQGHLAPLPLYVCPVHWAYDYTLRVYPVPDLLVIADKYDPFTVTNTDCLCINPGSFPRSGFAFKVFYPSSKTVEDSKLQGF

Target ORF information:

RefSeq Version	XM_011244018.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA directed), epsilon 2 (p59 subunit) (Pole2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244018.1

ORF Insert Sequence:

ATGATCGAAC GATCTGTCGT GGAGGCAGCT GTGCAAGAGT GCAGTCAGTC TATGGATGAG 
ACTATAGAGC ACGTTTTCAA TATCATTGGA GCTTTTGATA TTCCACGTTT TATATATAAT 
TCAGAAAGGA AAAAATTTCT TCCTCTATCC ATGACTAATC ATGCTGCACC AACCTTATTT 
GGAACAGCAA GAGACAAAGC CGAGCTGTAT CTTGAACGCT ATACCATTTT ACACCAGAGG 
ACACACAGAC ATGAATTGTT CACTCCTCCG GTGATAGGCT CACACCTTGA AGAAAGTGGG 
AGCAAGTTCC AGCTTAAAAC TATAGAAACC TTACTGGGTA GCACAAGCAA AGTTGGTGAC 
GTGATCGTCC TGGGGATGAT AACCCAGCTG AAAGAGGGGA AGTTTTTCCT GGAAGATCCT 
ACTGGAACAG TACAACTGGA CCTTAGTAAA GCTCAGTTCC ACAGTGGCTT ATACACCGAG 
GCCTGCTTCG TCCTGGCAGA AGGTTGGTTT GAAGATCAAG TGTTTCACGC CAATGCCTTT 
GGATTTCCTC CCACTGAGCC CTCCAGCACC ACAAGGGCGT ACTATGGAAA TATTAATTTT 
TTTGGAGGGC CTTCTAATGC ATCAGTGAAG ACGTATACAA AGCTGAGACA GCTGGAAGAT 
GAGAACAAGG ACGCCATGTT TGTGATTGTG TCTGATGTTT GGTTGGACCA AGTGCAAGTG 
TTGGAGAAGT TTCATGTAAT GTTTTCTGGT TACTCGCCAG CACCTCCAAC CTGCTTTATT 
CTGTGTGGTA ATTTTTCATC TGCACCATAT GGAAAAAATC AAGTCCAGGC TTTGAAAGAT 
TCCCTAAAAT CTTTGGCAGA CATAATTTGT GAATATCCAA GTATCCACCA AAGTAGCCGT 
TTTGTGTTTG TGCCTGGCCC AGAGGATCCT GGATTTGGTT CCATTTTACC AAGGCCGCCA 
CTCGCTGAAA GCATCACACA GGAATTCAGA CAAAGGGTTC CGTTTTCAGT TTTCACGACT 
AATCCTTGCA GAATTCAGTA TTGTACACAA GAAATAATTA TCTTCCGTGA AGACTTAGTG 
AATAAAATGT GTAGAAACTG TGTCCGTTTT CCTAGCAGCA GTTTGGATAT TCCTACTCAC 
TTCGTAAAGA CCATCTTATC TCAGGGACAT CTGGCTCCTC TCCCTCTTTA CGTATGCCCC 
GTCCACTGGG CATATGATTA CACGTTGAGA GTGTACCCGG TGCCCGACCT GCTGGTCATT 
GCAGACAAGT ACGACCCGTT CACCGTGACC AACACCGATT GCCTCTGCAT AAACCCTGGC 
TCCTTTCCAA GAAGTGGATT TGCATTCAAA GTTTTTTACC CTTCCAGTAA AACAGTAGAA 
GACAGCAAAC TTCAGGGCTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pole2 ( XM_006515598.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515661.1 Mus musculus polymerase (DNA directed), epsilon 2 (p59 subunit) (Pole2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu47668
Clone ID Related Accession (Same CDS sequence)	XM_006515598.3 , XM_006515598.4
Accession Version	XM_006515598.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	DNA polymerase epsilon subunit 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515598.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCGG AGCGGCTGCG GAGCCGCACG GTCTCAGCTT TCAAGCTGCG GGGCTTGCTG 
CTCAGGAGTG AAGCCACCAA GTATCTGACT GAAGCTCTCC AGTCTGTCAC TGAATTGGAG 
CTAGAGGAGA ACCTAGGAAA GATCATCGAT GCAGTTGAAA AGCAGCCCTT GTCCTCGAAC 
ATGATCGAAC GATCTGTCGT GGAGGCAGCT GTGCAAGAGT GCAGTCAGTC TATGGATGAG 
ACTATAGAGC ACGTTTTCAA TATCATTGGA GCTTTTGATA TTCCACGTTT TATATATAAT 
TCAGAAAGGA AAAAATTTCT TCCTCTATCC ATGACTAATC ATGCTGCACC AACCTTATTT 
GGAACAGCAA GAGACAAAGC CGAGCTGTAT CTTGAACGCT ATACCATTTT ACACCAGAGG 
ACACACAGAC ATGAATTGTT CACTCCTCCG GTGATAGGCT CACACCTTGA AGAAAGTGGG 
AGCAAGTTCC AGCTTAAAAC TATAGAAACC TTACTGGGTA GCACAAGCAA AGTTGGTGAC 
GTGATCGTCC TGGGGATGAT AACCCAGCTG AAAGAGGGGA AGTTTTTCCT GGAAGATCCT 
ACTGGAACAG TACAACTGGA CCTTAGTAAA GCTCAGTTCC ACAGTGGCTT ATACACCGAG 
GCCTGCTTCG TCCTGGCAGA AGGTTGGTTT GAAGATCAAG TGTTTCACGC CAATGCCTTT 
GGATTTCCTC CCACTGAGCC CTCCAGCACC ACAAGGGCGT ACTATGGAAA TATTAATTTT 
TTTGGAGGGC CTTCTAATGC ATCAGTGAAG ACGTATACAA AGCTGAGACA GCTGGAAGAT 
GAGAACAAGG ACGCCATGTT TGTGATTGTG TCTGATGTTT GGTTGGACCA AGTGCAAGTG 
TTGGAGAAGT TTCATGTAAT GTTTTCTGGT TACTCGCCAG CACCTCCAAC CTGCTTTATT 
CTGTGTGGTA ATTTTTCATC TGCACCATAT GGAAAAAATC AAGTCCAGGC TTTGAAAGAT 
TCCCTAAAAT CTTTGGCAGA CATAATTTGT GAATATCCAA GTATCCACCA AAGTAGCCGT 
TTTGTGTTTG TGCCTGGCCC AGAGGATCCT GGATTTGGTT CCATTTTACC AAGGCCGCCA 
CTCGCTGAAA GCATCACACA GGAATTCAGA CAAAGGGTTC CGTTTTCAGT TTTCACGACT 
AATCCTTGCA GTGGTATCTC CAGCTTCCTC AGGAATCCCA TCCTTGTGGG TCCAGATGTC 
CAGTGTCCTT TCTCTCCGGA TGCCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515661.1
CDS	42..1331
Translation	MAPERLRSRTVSAFKLRGLLLRSEATKYLTEALQSVTELELEENLGKIIDAVEKQPLSSNMIERSVVEAAVQECSQSMDETIEHVFNIIGAFDIPRFIYNSERKKFLPLSMTNHAAPTLFGTARDKAELYLERYTILHQRTHRHELFTPPVIGSHLEESGSKFQLKTIETLLGSTSKVGDVIVLGMITQLKEGKFFLEDPTGTVQLDLSKAQFHSGLYTEACFVLAEGWFEDQVFHANAFGFPPTEPSSTTRAYYGNINFFGGPSNASVKTYTKLRQLEDENKDAMFVIVSDVWLDQVQVLEKFHVMFSGYSPAPPTCFILCGNFSSAPYGKNQVQALKDSLKSLADIICEYPSIHQSSRFVFVPGPEDPGFGSILPRPPLAESITQEFRQRVPFSVFTTNPCSGISSFLRNPILVGPDVQCPFSPDAL

Target ORF information:

RefSeq Version	XM_006515598.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA directed), epsilon 2 (p59 subunit) (Pole2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515598.4

ORF Insert Sequence:

ATGGCGCCGG AGCGGCTGCG GAGCCGCACG GTCTCAGCTT TCAAGCTGCG GGGCTTGCTG 
CTCAGGAGTG AAGCCACCAA GTATCTGACT GAAGCTCTCC AGTCTGTCAC TGAATTGGAG 
CTAGAGGAGA ACCTAGGAAA GATCATCGAT GCAGTTGAAA AGCAGCCCTT GTCCTCGAAC 
ATGATCGAAC GATCTGTCGT GGAGGCAGCT GTGCAAGAGT GCAGTCAGTC TATGGATGAG 
ACTATAGAGC ACGTTTTCAA TATCATTGGA GCTTTTGATA TTCCACGTTT TATATATAAT 
TCAGAAAGGA AAAAATTTCT TCCTCTATCC ATGACTAATC ATGCTGCACC AACCTTATTT 
GGAACAGCAA GAGACAAAGC CGAGCTGTAT CTTGAACGCT ATACCATTTT ACACCAGAGG 
ACACACAGAC ATGAATTGTT CACTCCTCCG GTGATAGGCT CACACCTTGA AGAAAGTGGG 
AGCAAGTTCC AGCTTAAAAC TATAGAAACC TTACTGGGTA GCACAAGCAA AGTTGGTGAC 
GTGATCGTCC TGGGGATGAT AACCCAGCTG AAAGAGGGGA AGTTTTTCCT GGAAGATCCT 
ACTGGAACAG TACAACTGGA CCTTAGTAAA GCTCAGTTCC ACAGTGGCTT ATACACCGAG 
GCCTGCTTCG TCCTGGCAGA AGGTTGGTTT GAAGATCAAG TGTTTCACGC CAATGCCTTT 
GGATTTCCTC CCACTGAGCC CTCCAGCACC ACAAGGGCGT ACTATGGAAA TATTAATTTT 
TTTGGAGGGC CTTCTAATGC ATCAGTGAAG ACGTATACAA AGCTGAGACA GCTGGAAGAT 
GAGAACAAGG ACGCCATGTT TGTGATTGTG TCTGATGTTT GGTTGGACCA AGTGCAAGTG 
TTGGAGAAGT TTCATGTAAT GTTTTCTGGT TACTCGCCAG CACCTCCAAC CTGCTTTATT 
CTGTGTGGTA ATTTTTCATC TGCACCATAT GGAAAAAATC AAGTCCAGGC TTTGAAAGAT 
TCCCTAAAAT CTTTGGCAGA CATAATTTGT GAATATCCAA GTATCCACCA AAGTAGCCGT 
TTTGTGTTTG TGCCTGGCCC AGAGGATCCT GGATTTGGTT CCATTTTACC AAGGCCGCCA 
CTCGCTGAAA GCATCACACA GGAATTCAGA CAAAGGGTTC CGTTTTCAGT TTTCACGACT 
AATCCTTGCA GTGGTATCTC CAGCTTCCTC AGGAATCCCA TCCTTGTGGG TCCAGATGTC 
CAGTGTCCTT TCTCTCCGGA TGCCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pole2 ( XM_011244018.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242320.1 Mus musculus polymerase (DNA directed), epsilon 2 (p59 subunit) (Pole2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu68465
Clone ID Related Accession (Same CDS sequence)	XM_011244018.1 , XM_011244018.2
Accession Version	XM_011244018.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	DNA polymerase epsilon subunit 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011244018.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGAAC GATCTGTCGT GGAGGCAGCT GTGCAAGAGT GCAGTCAGTC TATGGATGAG 
ACTATAGAGC ACGTTTTCAA TATCATTGGA GCTTTTGATA TTCCACGTTT TATATATAAT 
TCAGAAAGGA AAAAATTTCT TCCTCTATCC ATGACTAATC ATGCTGCACC AACCTTATTT 
GGAACAGCAA GAGACAAAGC CGAGCTGTAT CTTGAACGCT ATACCATTTT ACACCAGAGG 
ACACACAGAC ATGAATTGTT CACTCCTCCG GTGATAGGCT CACACCTTGA AGAAAGTGGG 
AGCAAGTTCC AGCTTAAAAC TATAGAAACC TTACTGGGTA GCACAAGCAA AGTTGGTGAC 
GTGATCGTCC TGGGGATGAT AACCCAGCTG AAAGAGGGGA AGTTTTTCCT GGAAGATCCT 
ACTGGAACAG TACAACTGGA CCTTAGTAAA GCTCAGTTCC ACAGTGGCTT ATACACCGAG 
GCCTGCTTCG TCCTGGCAGA AGGTTGGTTT GAAGATCAAG TGTTTCACGC CAATGCCTTT 
GGATTTCCTC CCACTGAGCC CTCCAGCACC ACAAGGGCGT ACTATGGAAA TATTAATTTT 
TTTGGAGGGC CTTCTAATGC ATCAGTGAAG ACGTATACAA AGCTGAGACA GCTGGAAGAT 
GAGAACAAGG ACGCCATGTT TGTGATTGTG TCTGATGTTT GGTTGGACCA AGTGCAAGTG 
TTGGAGAAGT TTCATGTAAT GTTTTCTGGT TACTCGCCAG CACCTCCAAC CTGCTTTATT 
CTGTGTGGTA ATTTTTCATC TGCACCATAT GGAAAAAATC AAGTCCAGGC TTTGAAAGAT 
TCCCTAAAAT CTTTGGCAGA CATAATTTGT GAATATCCAA GTATCCACCA AAGTAGCCGT 
TTTGTGTTTG TGCCTGGCCC AGAGGATCCT GGATTTGGTT CCATTTTACC AAGGCCGCCA 
CTCGCTGAAA GCATCACACA GGAATTCAGA CAAAGGGTTC CGTTTTCAGT TTTCACGACT 
AATCCTTGCA GAATTCAGTA TTGTACACAA GAAATAATTA TCTTCCGTGA AGACTTAGTG 
AATAAAATGT GTAGAAACTG TGTCCGTTTT CCTAGCAGCA GTTTGGATAT TCCTACTCAC 
TTCGTAAAGA CCATCTTATC TCAGGGACAT CTGGCTCCTC TCCCTCTTTA CGTATGCCCC 
GTCCACTGGG CATATGATTA CACGTTGAGA GTGTACCCGG TGCCCGACCT GCTGGTCATT 
GCAGACAAGT ACGACCCGTT CACCGTGACC AACACCGATT GCCTCTGCAT AAACCCTGGC 
TCCTTTCCAA GAAGTGGATT TGCATTCAAA GTTTTTTACC CTTCCAGTAA AACAGTAGAA 
GACAGCAAAC TTCAGGGCTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242320.1
CDS	216..1619
Translation	MIERSVVEAAVQECSQSMDETIEHVFNIIGAFDIPRFIYNSERKKFLPLSMTNHAAPTLFGTARDKAELYLERYTILHQRTHRHELFTPPVIGSHLEESGSKFQLKTIETLLGSTSKVGDVIVLGMITQLKEGKFFLEDPTGTVQLDLSKAQFHSGLYTEACFVLAEGWFEDQVFHANAFGFPPTEPSSTTRAYYGNINFFGGPSNASVKTYTKLRQLEDENKDAMFVIVSDVWLDQVQVLEKFHVMFSGYSPAPPTCFILCGNFSSAPYGKNQVQALKDSLKSLADIICEYPSIHQSSRFVFVPGPEDPGFGSILPRPPLAESITQEFRQRVPFSVFTTNPCRIQYCTQEIIIFREDLVNKMCRNCVRFPSSSLDIPTHFVKTILSQGHLAPLPLYVCPVHWAYDYTLRVYPVPDLLVIADKYDPFTVTNTDCLCINPGSFPRSGFAFKVFYPSSKTVEDSKLQGF

Target ORF information:

RefSeq Version	XM_011244018.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA directed), epsilon 2 (p59 subunit) (Pole2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244018.2

ORF Insert Sequence:

ATGATCGAAC GATCTGTCGT GGAGGCAGCT GTGCAAGAGT GCAGTCAGTC TATGGATGAG 
ACTATAGAGC ACGTTTTCAA TATCATTGGA GCTTTTGATA TTCCACGTTT TATATATAAT 
TCAGAAAGGA AAAAATTTCT TCCTCTATCC ATGACTAATC ATGCTGCACC AACCTTATTT 
GGAACAGCAA GAGACAAAGC CGAGCTGTAT CTTGAACGCT ATACCATTTT ACACCAGAGG 
ACACACAGAC ATGAATTGTT CACTCCTCCG GTGATAGGCT CACACCTTGA AGAAAGTGGG 
AGCAAGTTCC AGCTTAAAAC TATAGAAACC TTACTGGGTA GCACAAGCAA AGTTGGTGAC 
GTGATCGTCC TGGGGATGAT AACCCAGCTG AAAGAGGGGA AGTTTTTCCT GGAAGATCCT 
ACTGGAACAG TACAACTGGA CCTTAGTAAA GCTCAGTTCC ACAGTGGCTT ATACACCGAG 
GCCTGCTTCG TCCTGGCAGA AGGTTGGTTT GAAGATCAAG TGTTTCACGC CAATGCCTTT 
GGATTTCCTC CCACTGAGCC CTCCAGCACC ACAAGGGCGT ACTATGGAAA TATTAATTTT 
TTTGGAGGGC CTTCTAATGC ATCAGTGAAG ACGTATACAA AGCTGAGACA GCTGGAAGAT 
GAGAACAAGG ACGCCATGTT TGTGATTGTG TCTGATGTTT GGTTGGACCA AGTGCAAGTG 
TTGGAGAAGT TTCATGTAAT GTTTTCTGGT TACTCGCCAG CACCTCCAAC CTGCTTTATT 
CTGTGTGGTA ATTTTTCATC TGCACCATAT GGAAAAAATC AAGTCCAGGC TTTGAAAGAT 
TCCCTAAAAT CTTTGGCAGA CATAATTTGT GAATATCCAA GTATCCACCA AAGTAGCCGT 
TTTGTGTTTG TGCCTGGCCC AGAGGATCCT GGATTTGGTT CCATTTTACC AAGGCCGCCA 
CTCGCTGAAA GCATCACACA GGAATTCAGA CAAAGGGTTC CGTTTTCAGT TTTCACGACT 
AATCCTTGCA GAATTCAGTA TTGTACACAA GAAATAATTA TCTTCCGTGA AGACTTAGTG 
AATAAAATGT GTAGAAACTG TGTCCGTTTT CCTAGCAGCA GTTTGGATAT TCCTACTCAC 
TTCGTAAAGA CCATCTTATC TCAGGGACAT CTGGCTCCTC TCCCTCTTTA CGTATGCCCC 
GTCCACTGGG CATATGATTA CACGTTGAGA GTGTACCCGG TGCCCGACCT GCTGGTCATT 
GCAGACAAGT ACGACCCGTT CACCGTGACC AACACCGATT GCCTCTGCAT AAACCCTGGC 
TCCTTTCCAA GAAGTGGATT TGCATTCAAA GTTTTTTACC CTTCCAGTAA AACAGTAGAA 
GACAGCAAAC TTCAGGGCTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of FEN1 S187 phosphorylation in counteracting oxygen-induced stress and regulating postnatal heart development.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(1)132-147(2017 01)
Zhou L,Dai H,Wu J,Zhou M,Yuan H,Du J,Yang L,Wu X,Xu H,Hua Y,Xu J,Zheng L,Shen B

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

The second largest subunit of mouse DNA polymerase epsilon, DPE2, interacts with SAP18 and recruits the Sin3 co-repressor protein to DNA.
Journal of biochemistry131(3)307-11(2002 Mar)
Wada M,Miyazawa H,Wang RS,Mizuno T,Sato A,Asashima M,Hanaoka F

The small subunits of human and mouse DNA polymerase epsilon are homologous to the second largest subunit of the yeast Saccharomyces cerevisiae DNA polymerase epsilon.
Nucleic acids research26(3)730-4(1998 Feb)
Jokela M,M?kiniemi M,Lehtonen S,Szpirer C,Hellman U,Syv?oja JE

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Pole2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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