Ncam2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ncam2
Entrez Gene ID	17968
Full Name	neural cell adhesion molecule 2
Synonyms	Ncam-2,Ocam,RNCAM
General protein information	Preferred Names neural cell adhesion molecule 2 Names neural cell adhesion molecule 2 N-CAM-2 R4B12 antigen neural cell adhesion molecule RB-8 olfactory axon cell adhesion molecule
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16 C3.3\|16 45.89 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001113208.1	NP_001106679.1	neural cell adhesion molecule 2 isoform a precursor
NM_010954.4	NP_035084.1	neural cell adhesion molecule 2 isoform b precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	NCAM2	XP_425540.4
Homo sapiens (human)	NCAM2	NP_004531.2
Bos taurus (cattle)	NCAM2	XP_002684681.3
Mus musculus (house mouse)	Ncam2	NP_001106679.1
Danio rerio (zebrafish)	ncam2	NP_571905.1
	NCAM2	XP_001106060.1
Canis lupus familiaris (dog)	NCAM2	XP_544829.4
Xenopus tropicalis (tropical clawed frog)	ncam2	NP_001120897.1
Pan troglodytes (chimpanzee)	NCAM2	XP_531397.2
Rattus norvegicus (Norway rat)	Ncam2	NP_981954.1
Danio rerio (zebrafish)	zgc:152904	NP_001070212.1

Gene Ontology
Bibliography

Related articles in PubMed

Tag1 deficiency results in olfactory dysfunction through impaired migration of mitral cells.
Bastakis GG, Savvaki M, Stamatakis A, Vidaki M, Karagogeos D
Development (Cambridge, England)142(24)4318-28(2015 Dec)

A?-dependent reduction of NCAM2-mediated synaptic adhesion contributes to synapse loss in Alzheimer's disease.
Leshchyns'ka I, Liew HT, Shepherd C, Halliday GM, Stevens CH, Ke YD, Ittner LM, Sytnyk V
Nature communications68836(2015 Nov)

OCAM regulates embryonic spinal cord stem cell proliferation by modulating ErbB2 receptor.
Deleyrolle L, Sabourin JC, Rothhut B, Fujita H, Guichet PO, Teigell M, Ripoll C, Chauvet N, Perrin F, Mamaeva D, Noda T, Mori K, Yoshihara Y, Hugnot JP
PloS one10(4)e0122337(2015)

Neural cell adhesion molecule 2 promotes the formation of filopodia and neurite branching by inducing submembrane increases in Ca2+ levels.
Sheng L, Leshchyns'ka I, Sytnyk V
The Journal of neuroscience : the official journal of the Society for Neuroscience35(4)1739-52(2015 Jan)

Expression of the immunoglobulin superfamily cell adhesion molecules in the developing spinal cord and dorsal root ganglion.
Gu Z, Imai F, Kim IJ, Fujita H, Katayama Ki, Mori K, Yoshihara Y, Yoshida Y
PloS one10(3)e0121550(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Abeta-dependent disruption of NCAM2 functions in Alzheimer's disease hippocampus contributes to synapse loss.
Title: A?-dependent reduction of NCAM2-mediated synaptic adhesion contributes to synapse loss in Alzheimer's disease.

These results indicate that OCAM exerts a posttranscriptional control on the ErbB2 signalling in spinal cord stem cells. This study adds further support for considering cell adhesion molecules as regulators of the ErbB signalling
Title: OCAM regulates embryonic spinal cord stem cell proliferation by modulating ErbB2 receptor.

This study identified the cell adhesion molecule NCAM2 as a new regulator of filopodial formation and neurite branching.
Title: Neural cell adhesion molecule 2 promotes the formation of filopodia and neurite branching by inducing submembrane increases in Ca2+ levels.

Two novel Mmatrix metallopeptidase 9 targets were identified: synaptic cell adhesion molecule-2 and collapsin response mediator protein-2
Title: Synaptic cell adhesion molecule-2 and collapsin response mediator protein-2 are novel members of the matrix metalloproteinase-9 degradome.

Loss of olfactory cell adhesion molecule reduces the synchrony of mitral cell activity in olfactory glomeruli.
Title: Loss of olfactory cell adhesion molecule reduces the synchrony of mitral cell activity in olfactory glomeruli.

The following Ncam2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ncam2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu23485	NM_001113208.1 Latest version!	Mus musculus neural cell adhesion molecule 2 (Ncam2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$496.30 $709.00
OMu23487	NM_010954.4 Latest version!	Mus musculus neural cell adhesion molecule 2 (Ncam2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ncam2 ( NM_001113208.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001106679.1 Mus musculus neural cell adhesion molecule 2 (Ncam2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu23485
Clone ID Related Accession (Same CDS sequence)	NM_001113208.1
Accession Version	NM_001113208.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	neural cell adhesion molecule 2 isoform a precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AW121550.1, BC119027.1, AF016620.1, BB663005.1 and BM933307.1. Transcript Variant: This variant (1) encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF001287.1, SRR1660811.94494.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCCTCC TCCTCTCCTT CTACCTGCTC GGGTTGCTTG TCAGGAGCGG GCAAGCTCTT 
CTTCAAGTGA CAATTTCACT TAGCAAAGTA GAGCTTAGTG TGGGTGAGTC TAAATTCTTC 
ACATGTACAG CCATTGGTGA GCCTGAGAGT ATCGACTGGT ATAACCCTCA AGGAGAGAAG 
ATCATTTCAA CGCAGAGGGT TATGCTACAG AAGGAGGGTG TCAGGTCACG ACTCACCATC 
TACAATGCAA ACATAGAAGA TGCAGGGATA TATCGCTGTC AAGCGACAGA TGCCAAAGGA 
CAGACACAAG AAGCTACAGT AGTTTTGGAA ATTTACCAAA AACTCACCTT CAGAGAAGTG 
GTGTCCCCTC AAGAGTTCAA GCAAGGGGAG GATGCAGAAG TGGTTTGCAG AGTGAGCAGT 
TCCCCAGCCC CTGCGGTCAG CTGGTTGTAC CACAATGAGG AAGTCACCAC CATCCCCGAC 
AATCGGTTTG CAGTGCTTGC AAACAATAAT TTGCAGATCC TCAATATCAA TAAAAGTGAT 
GAAGGTATAT ACAGATGTGA AGGAAGAGTG GAGGCGAGGG GAGAGATTGA CTTCCGGGAT 
ATCATTGTTA TTGTTAACGT TCCACCAGCA ATCATGATGC CCCAGAAGTC CTTCAATGCT 
ACAGCAGAGA GAGGAGAAGA GATGACCTTA ACATGCAAGG CCTCGGGCTC CCCAGATCCA 
ACCATCTCTT GGTTCAGGAA CGGCAAACTC ATTGAAGAAA ATGAAAAGTA TATTTTAAAG 
GGCAGTAATA CAGAGCTCAC TGTCAGGAAC ATAATCAATA AAGACGGGGG CTCTTATGTC 
TGCAAAGCAA CAAACAAGGC AGGAGAAGAT CAAAAGCAGG CCTTCCTTCA AGTCTTTGTA 
CAGCCTCATA TATTACAACT TAAAAATGAG ACAACATCTG AGAATGGTCA TGTCACACTC 
GTATGTGAAG CAGAAGGGGA GCCTGTTCCA GAAATCACAT GGAAAAGAGC CATAGATGGA 
GTCATGTTTT CTGAAGGTGA TAAGAGTCCG GATGGCCGCA TCGAAGTTAA AGGGCAGCAT 
GGACGCTCTT CACTGCACAT TAGAGATGTG AAGTTGTCAG ATTCGGGGAG ATATGACTGT 
GAGGCCGCTA GTCGGATTGG TGGGCACCAG AGGAGCATGC ACCTGGACAT CGAATATGCT 
CCTAAGTTTG TTTCAAATCA GACAATGTAT TACTCTTGGG AAGGAAATCC AATCAACATA 
AGTTGTGATG TGACAGCAAA CCCACCTGCG TCAATCCACT GGAGAAGAGA GAAACTGCTC 
TTACCAGCTA AGAATACGAC TCATTTAAAA ACTCACAGTG TAGGAAGAAA GATGATACTC 
GAGATTGCCC CCACATCAGA CAATGACTTT GGACGATATA ACTGCACAGC TACTAACCGT 
ATAGGCACAA GATTTCAGGA ATATATTCTT GAATTAGCAG ATGTCCCCTC TAGTCCCCAT 
GGAGTGAAGA TTATAGAGCT GTCACAGACC ACAGCCAAGA TCTCTTTCAA TAAACCCGAA 
TCCCATGGAG GTGTGCCTAT TCATCACTAT CAAGTGGATG TCAAAGAAGT GGCATCAGAA 
ACCTGGAAAA TAGTACGCTC CCATGGAGTT CAAACGATGG TTGTTTTGAG CAGTCTGGAA 
CCAAATACGA CTTACGAAAT TAGGGTTGCA GCAGTGAATG GCAAAGGTCA AGGAGACTAC 
AGTAAAATAG AAATATTCCA GACACTGCCA GTCCGTGAGC CAAGTCCCCC TTCCATACAT 
GGACAGCCAA GCAGTGGCAA GAGTTTTAAA ATCAGCATCA CCAAGCAAGA TGATGGAGGG 
GCTCCTATTT TAGAATACAT TGTAAAATAC AGAAGTAAAG ACAAGGAAGA TCAGTGGCTA 
GAGAAGAAGG TACAGGGAAA TAAAGACCAC ATTATCTTGG AGCATCTGCA GTGGACAATG 
GGCTACGAAG TGCAAATCAC AGCTGCCAAC AGACTGGGAT ATTCTGAGCC TACTGTATAT 
GAGTTCAGCA TGCCCCCAAA ACCCAACATT ATTAAAGACA CACTTTTTAA TGGCCTTGGC 
CTAGGAGCCA TCATTGGCCT TGGAGTTGCT GCCCTTTTGC TAATCCTTGT GGTAACAGAC 
GTCAGCTGCT TCTTCATTCG ACAATGTGGG TTACTAATGT GCATCACCAG GAGAATGTGC 
GGGAAAAAGA GTGGCTCCAG TGGGAAAAGT AAAGAACTTG AAGAAGGAAA AGCAGCTTAC 
CTGAAAGATG GATCAAAAGA ACCGATAGTG GAAATGAGAA CAGAAGATGA AAGAATCACT 
AACCATGAAG ATGGGAGTCC AGTAAATGAG CCAAATGAAA CCACACCGCT AACAGAACCT 
GAAAAATTGC CTTTAAAAGA AGAAAACGGA AAGGAAGTTT TAAACGCAGA AACTATAGAA 
ATTAAAGTGT CCAATGACAT CATCCAGTCT AAAGAAGATG ACATAAAGGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106679.1
CDS	218..2731
Translation	MSLLLSFYLLGLLVRSGQALLQVTISLSKVELSVGESKFFTCTAIGEPESIDWYNPQGEKIISTQRVMLQKEGVRSRLTIYNANIEDAGIYRCQATDAKGQTQEATVVLEIYQKLTFREVVSPQEFKQGEDAEVVCRVSSSPAPAVSWLYHNEEVTTIPDNRFAVLANNNLQILNINKSDEGIYRCEGRVEARGEIDFRDIIVIVNVPPAIMMPQKSFNATAERGEEMTLTCKASGSPDPTISWFRNGKLIEENEKYILKGSNTELTVRNIINKDGGSYVCKATNKAGEDQKQAFLQVFVQPHILQLKNETTSENGHVTLVCEAEGEPVPEITWKRAIDGVMFSEGDKSPDGRIEVKGQHGRSSLHIRDVKLSDSGRYDCEAASRIGGHQRSMHLDIEYAPKFVSNQTMYYSWEGNPINISCDVTANPPASIHWRREKLLLPAKNTTHLKTHSVGRKMILEIAPTSDNDFGRYNCTATNRIGTRFQEYILELADVPSSPHGVKIIELSQTTAKISFNKPESHGGVPIHHYQVDVKEVASETWKIVRSHGVQTMVVLSSLEPNTTYEIRVAAVNGKGQGDYSKIEIFQTLPVREPSPPSIHGQPSSGKSFKISITKQDDGGAPILEYIVKYRSKDKEDQWLEKKVQGNKDHIILEHLQWTMGYEVQITAANRLGYSEPTVYEFSMPPKPNIIKDTLFNGLGLGAIIGLGVAALLLILVVTDVSCFFIRQCGLLMCITRRMCGKKSGSSGKSKELEEGKAAYLKDGSKEPIVEMRTEDERITNHEDGSPVNEPNETTPLTEPEKLPLKEENGKEVLNAETIEIKVSNDIIQSKEDDIKA

Target ORF information:

RefSeq Version	NM_001113208.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neural cell adhesion molecule 2 (Ncam2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113208.1

ORF Insert Sequence:

ATGAGCCTCC TCCTCTCCTT CTACCTGCTC GGGTTGCTTG TCAGGAGCGG GCAAGCTCTT 
CTTCAAGTGA CAATTTCACT TAGCAAAGTA GAGCTTAGTG TGGGTGAGTC TAAATTCTTC 
ACATGTACAG CCATTGGTGA GCCTGAGAGT ATCGACTGGT ATAACCCTCA AGGAGAGAAG 
ATCATTTCAA CGCAGAGGGT TATGCTACAG AAGGAGGGTG TCAGGTCACG ACTCACCATC 
TACAATGCAA ACATAGAAGA TGCAGGGATA TATCGCTGTC AAGCGACAGA TGCCAAAGGA 
CAGACACAAG AAGCTACAGT AGTTTTGGAA ATTTACCAAA AACTCACCTT CAGAGAAGTG 
GTGTCCCCTC AAGAGTTCAA GCAAGGGGAG GATGCAGAAG TGGTTTGCAG AGTGAGCAGT 
TCCCCAGCCC CTGCGGTCAG CTGGTTGTAC CACAATGAGG AAGTCACCAC CATCCCCGAC 
AATCGGTTTG CAGTGCTTGC AAACAATAAT TTGCAGATCC TCAATATCAA TAAAAGTGAT 
GAAGGTATAT ACAGATGTGA AGGAAGAGTG GAGGCGAGGG GAGAGATTGA CTTCCGGGAT 
ATCATTGTTA TTGTTAACGT TCCACCAGCA ATCATGATGC CCCAGAAGTC CTTCAATGCT 
ACAGCAGAGA GAGGAGAAGA GATGACCTTA ACATGCAAGG CCTCGGGCTC CCCAGATCCA 
ACCATCTCTT GGTTCAGGAA CGGCAAACTC ATTGAAGAAA ATGAAAAGTA TATTTTAAAG 
GGCAGTAATA CAGAGCTCAC TGTCAGGAAC ATAATCAATA AAGACGGGGG CTCTTATGTC 
TGCAAAGCAA CAAACAAGGC AGGAGAAGAT CAAAAGCAGG CCTTCCTTCA AGTCTTTGTA 
CAGCCTCATA TATTACAACT TAAAAATGAG ACAACATCTG AGAATGGTCA TGTCACACTC 
GTATGTGAAG CAGAAGGGGA GCCTGTTCCA GAAATCACAT GGAAAAGAGC CATAGATGGA 
GTCATGTTTT CTGAAGGTGA TAAGAGTCCG GATGGCCGCA TCGAAGTTAA AGGGCAGCAT 
GGACGCTCTT CACTGCACAT TAGAGATGTG AAGTTGTCAG ATTCGGGGAG ATATGACTGT 
GAGGCCGCTA GTCGGATTGG TGGGCACCAG AGGAGCATGC ACCTGGACAT CGAATATGCT 
CCTAAGTTTG TTTCAAATCA GACAATGTAT TACTCTTGGG AAGGAAATCC AATCAACATA 
AGTTGTGATG TGACAGCAAA CCCACCTGCG TCAATCCACT GGAGAAGAGA GAAACTGCTC 
TTACCAGCTA AGAATACGAC TCATTTAAAA ACTCACAGTG TAGGAAGAAA GATGATACTC 
GAGATTGCCC CCACATCAGA CAATGACTTT GGACGATATA ACTGCACAGC TACTAACCGT 
ATAGGCACAA GATTTCAGGA ATATATTCTT GAATTAGCAG ATGTCCCCTC TAGTCCCCAT 
GGAGTGAAGA TTATAGAGCT GTCACAGACC ACAGCCAAGA TCTCTTTCAA TAAACCCGAA 
TCCCATGGAG GTGTGCCTAT TCATCACTAT CAAGTGGATG TCAAAGAAGT GGCATCAGAA 
ACCTGGAAAA TAGTACGCTC CCATGGAGTT CAAACGATGG TTGTTTTGAG CAGTCTGGAA 
CCAAATACGA CTTACGAAAT TAGGGTTGCA GCAGTGAATG GCAAAGGTCA AGGAGACTAC 
AGTAAAATAG AAATATTCCA GACACTGCCA GTCCGTGAGC CAAGTCCCCC TTCCATACAT 
GGACAGCCAA GCAGTGGCAA GAGTTTTAAA ATCAGCATCA CCAAGCAAGA TGATGGAGGG 
GCTCCTATTT TAGAATACAT TGTAAAATAC AGAAGTAAAG ACAAGGAAGA TCAGTGGCTA 
GAGAAGAAGG TACAGGGAAA TAAAGACCAC ATTATCTTGG AGCATCTGCA GTGGACAATG 
GGCTACGAAG TGCAAATCAC AGCTGCCAAC AGACTGGGAT ATTCTGAGCC TACTGTATAT 
GAGTTCAGCA TGCCCCCAAA ACCCAACATT ATTAAAGACA CACTTTTTAA TGGCCTTGGC 
CTAGGAGCCA TCATTGGCCT TGGAGTTGCT GCCCTTTTGC TAATCCTTGT GGTAACAGAC 
GTCAGCTGCT TCTTCATTCG ACAATGTGGG TTACTAATGT GCATCACCAG GAGAATGTGC 
GGGAAAAAGA GTGGCTCCAG TGGGAAAAGT AAAGAACTTG AAGAAGGAAA AGCAGCTTAC 
CTGAAAGATG GATCAAAAGA ACCGATAGTG GAAATGAGAA CAGAAGATGA AAGAATCACT 
AACCATGAAG ATGGGAGTCC AGTAAATGAG CCAAATGAAA CCACACCGCT AACAGAACCT 
GAAAAATTGC CTTTAAAAGA AGAAAACGGA AAGGAAGTTT TAAACGCAGA AACTATAGAA 
ATTAAAGTGT CCAATGACAT CATCCAGTCT AAAGAAGATG ACATAAAGGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ncam2 ( NM_010954.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035084.1 Mus musculus neural cell adhesion molecule 2 (Ncam2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu23487
Clone ID Related Accession (Same CDS sequence)	NM_010954.4
Accession Version	NM_010954.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Mus musculus(house mouse)
Product	neural cell adhesion molecule 2 isoform b precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AW121550.1, BC119027.1 and AC123849.3. On Dec 29, 2007 this sequence version replaced NM_010954.3. Transcript Variant: This variant (2) lacks several exons and includes an alternate 3' terminal exon, compared to variant 1. It encodes isoform b which is shorter and has a distinct C-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF001286.1, AF016619.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

ATGAGCCTCC TCCTCTCCTT CTACCTGCTC GGGTTGCTTG TCAGGAGCGG GCAAGCTCTT 
CTTCAAGTGA CAATTTCACT TAGCAAAGTA GAGCTTAGTG TGGGTGAGTC TAAATTCTTC 
ACATGTACAG CCATTGGTGA GCCTGAGAGT ATCGACTGGT ATAACCCTCA AGGAGAGAAG 
ATCATTTCAA CGCAGAGGGT TATGCTACAG AAGGAGGGTG TCAGGTCACG ACTCACCATC 
TACAATGCAA ACATAGAAGA TGCAGGGATA TATCGCTGTC AAGCGACAGA TGCCAAAGGA 
CAGACACAAG AAGCTACAGT AGTTTTGGAA ATTTACCAAA AACTCACCTT CAGAGAAGTG 
GTGTCCCCTC AAGAGTTCAA GCAAGGGGAG GATGCAGAAG TGGTTTGCAG AGTGAGCAGT 
TCCCCAGCCC CTGCGGTCAG CTGGTTGTAC CACAATGAGG AAGTCACCAC CATCCCCGAC 
AATCGGTTTG CAGTGCTTGC AAACAATAAT TTGCAGATCC TCAATATCAA TAAAAGTGAT 
GAAGGTATAT ACAGATGTGA AGGAAGAGTG GAGGCGAGGG GAGAGATTGA CTTCCGGGAT 
ATCATTGTTA TTGTTAACGT TCCACCAGCA ATCATGATGC CCCAGAAGTC CTTCAATGCT 
ACAGCAGAGA GAGGAGAAGA GATGACCTTA ACATGCAAGG CCTCGGGCTC CCCAGATCCA 
ACCATCTCTT GGTTCAGGAA CGGCAAACTC ATTGAAGAAA ATGAAAAGTA TATTTTAAAG 
GGCAGTAATA CAGAGCTCAC TGTCAGGAAC ATAATCAATA AAGACGGGGG CTCTTATGTC 
TGCAAAGCAA CAAACAAGGC AGGAGAAGAT CAAAAGCAGG CCTTCCTTCA AGTCTTTGTA 
CAGCCTCATA TATTACAACT TAAAAATGAG ACAACATCTG AGAATGGTCA TGTCACACTC 
GTATGTGAAG CAGAAGGGGA GCCTGTTCCA GAAATCACAT GGAAAAGAGC CATAGATGGA 
GTCATGTTTT CTGAAGGTGA TAAGAGTCCG GATGGCCGCA TCGAAGTTAA AGGGCAGCAT 
GGACGCTCTT CACTGCACAT TAGAGATGTG AAGTTGTCAG ATTCGGGGAG ATATGACTGT 
GAGGCCGCTA GTCGGATTGG TGGGCACCAG AGGAGCATGC ACCTGGACAT CGAATATGCT 
CCTAAGTTTG TTTCAAATCA GACAATGTAT TACTCTTGGG AAGGAAATCC AATCAACATA 
AGTTGTGATG TGACAGCAAA CCCACCTGCG TCAATCCACT GGAGAAGAGA GAAACTGCTC 
TTACCAGCTA AGAATACGAC TCATTTAAAA ACTCACAGTG TAGGAAGAAA GATGATACTC 
GAGATTGCCC CCACATCAGA CAATGACTTT GGACGATATA ACTGCACAGC TACTAACCGT 
ATAGGCACAA GATTTCAGGA ATATATTCTT GAATTAGCAG ATGTCCCCTC TAGTCCCCAT 
GGAGTGAAGA TTATAGAGCT GTCACAGACC ACAGCCAAGA TCTCTTTCAA TAAACCCGAA 
TCCCATGGAG GTGTGCCTAT TCATCACTAT CAAGTGGATG TCAAAGAAGT GGCATCAGAA 
ACCTGGAAAA TAGTACGCTC CCATGGAGTT CAAACGATGG TTGTTTTGAG CAGTCTGGAA 
CCAAATACGA CTTACGAAAT TAGGGTTGCA GCAGTGAATG GCAAAGGTCA AGGAGACTAC 
AGTAAAATAG AAATATTCCA GACACTGCCA GTCCGTGAGC CAAGTCCCCC TTCCATACAT 
GGACAGCCAA GCAGTGGCAA GAGTTTTAAA ATCAGCATCA CCAAGCAAGA TGATGGAGGG 
GCTCCTATTT TAGAATACAT TGTAAAATAC AGAAGTAAAG ACAAGGAAGA TCAGTGGCTA 
GAGAAGAAGG TACAGGGAAA TAAAGACCAC ATTATCTTGG AGCATCTGCA GTGGACAATG 
GGCTACGAAG TGCAAATCAC AGCTGCCAAC AGACTGGGAT ATTCTGAGCC TACTGTATAT 
GAGTTCAGCA TGCCCCCAAA ACCCAACATT ATTAAAGATA ATTGCTGTGA AGCGAATAAA 
GGTGAAAATG GAGGCCAGTC ATGGCATCTG AATGCTGTTG GGTTTACTTT TGTAATAACC 
ATGAGTTTGT CTTGTTTGTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035084.1
CDS	218..2401
Translation	MSLLLSFYLLGLLVRSGQALLQVTISLSKVELSVGESKFFTCTAIGEPESIDWYNPQGEKIISTQRVMLQKEGVRSRLTIYNANIEDAGIYRCQATDAKGQTQEATVVLEIYQKLTFREVVSPQEFKQGEDAEVVCRVSSSPAPAVSWLYHNEEVTTIPDNRFAVLANNNLQILNINKSDEGIYRCEGRVEARGEIDFRDIIVIVNVPPAIMMPQKSFNATAERGEEMTLTCKASGSPDPTISWFRNGKLIEENEKYILKGSNTELTVRNIINKDGGSYVCKATNKAGEDQKQAFLQVFVQPHILQLKNETTSENGHVTLVCEAEGEPVPEITWKRAIDGVMFSEGDKSPDGRIEVKGQHGRSSLHIRDVKLSDSGRYDCEAASRIGGHQRSMHLDIEYAPKFVSNQTMYYSWEGNPINISCDVTANPPASIHWRREKLLLPAKNTTHLKTHSVGRKMILEIAPTSDNDFGRYNCTATNRIGTRFQEYILELADVPSSPHGVKIIELSQTTAKISFNKPESHGGVPIHHYQVDVKEVASETWKIVRSHGVQTMVVLSSLEPNTTYEIRVAAVNGKGQGDYSKIEIFQTLPVREPSPPSIHGQPSSGKSFKISITKQDDGGAPILEYIVKYRSKDKEDQWLEKKVQGNKDHIILEHLQWTMGYEVQITAANRLGYSEPTVYEFSMPPKPNIIKDNCCEANKGENGGQSWHLNAVGFTFVITMSLSCLF

Target ORF information:

RefSeq Version	NM_010954.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus neural cell adhesion molecule 2 (Ncam2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010954.4

ORF Insert Sequence:

ATGAGCCTCC TCCTCTCCTT CTACCTGCTC GGGTTGCTTG TCAGGAGCGG GCAAGCTCTT 
CTTCAAGTGA CAATTTCACT TAGCAAAGTA GAGCTTAGTG TGGGTGAGTC TAAATTCTTC 
ACATGTACAG CCATTGGTGA GCCTGAGAGT ATCGACTGGT ATAACCCTCA AGGAGAGAAG 
ATCATTTCAA CGCAGAGGGT TATGCTACAG AAGGAGGGTG TCAGGTCACG ACTCACCATC 
TACAATGCAA ACATAGAAGA TGCAGGGATA TATCGCTGTC AAGCGACAGA TGCCAAAGGA 
CAGACACAAG AAGCTACAGT AGTTTTGGAA ATTTACCAAA AACTCACCTT CAGAGAAGTG 
GTGTCCCCTC AAGAGTTCAA GCAAGGGGAG GATGCAGAAG TGGTTTGCAG AGTGAGCAGT 
TCCCCAGCCC CTGCGGTCAG CTGGTTGTAC CACAATGAGG AAGTCACCAC CATCCCCGAC 
AATCGGTTTG CAGTGCTTGC AAACAATAAT TTGCAGATCC TCAATATCAA TAAAAGTGAT 
GAAGGTATAT ACAGATGTGA AGGAAGAGTG GAGGCGAGGG GAGAGATTGA CTTCCGGGAT 
ATCATTGTTA TTGTTAACGT TCCACCAGCA ATCATGATGC CCCAGAAGTC CTTCAATGCT 
ACAGCAGAGA GAGGAGAAGA GATGACCTTA ACATGCAAGG CCTCGGGCTC CCCAGATCCA 
ACCATCTCTT GGTTCAGGAA CGGCAAACTC ATTGAAGAAA ATGAAAAGTA TATTTTAAAG 
GGCAGTAATA CAGAGCTCAC TGTCAGGAAC ATAATCAATA AAGACGGGGG CTCTTATGTC 
TGCAAAGCAA CAAACAAGGC AGGAGAAGAT CAAAAGCAGG CCTTCCTTCA AGTCTTTGTA 
CAGCCTCATA TATTACAACT TAAAAATGAG ACAACATCTG AGAATGGTCA TGTCACACTC 
GTATGTGAAG CAGAAGGGGA GCCTGTTCCA GAAATCACAT GGAAAAGAGC CATAGATGGA 
GTCATGTTTT CTGAAGGTGA TAAGAGTCCG GATGGCCGCA TCGAAGTTAA AGGGCAGCAT 
GGACGCTCTT CACTGCACAT TAGAGATGTG AAGTTGTCAG ATTCGGGGAG ATATGACTGT 
GAGGCCGCTA GTCGGATTGG TGGGCACCAG AGGAGCATGC ACCTGGACAT CGAATATGCT 
CCTAAGTTTG TTTCAAATCA GACAATGTAT TACTCTTGGG AAGGAAATCC AATCAACATA 
AGTTGTGATG TGACAGCAAA CCCACCTGCG TCAATCCACT GGAGAAGAGA GAAACTGCTC 
TTACCAGCTA AGAATACGAC TCATTTAAAA ACTCACAGTG TAGGAAGAAA GATGATACTC 
GAGATTGCCC CCACATCAGA CAATGACTTT GGACGATATA ACTGCACAGC TACTAACCGT 
ATAGGCACAA GATTTCAGGA ATATATTCTT GAATTAGCAG ATGTCCCCTC TAGTCCCCAT 
GGAGTGAAGA TTATAGAGCT GTCACAGACC ACAGCCAAGA TCTCTTTCAA TAAACCCGAA 
TCCCATGGAG GTGTGCCTAT TCATCACTAT CAAGTGGATG TCAAAGAAGT GGCATCAGAA 
ACCTGGAAAA TAGTACGCTC CCATGGAGTT CAAACGATGG TTGTTTTGAG CAGTCTGGAA 
CCAAATACGA CTTACGAAAT TAGGGTTGCA GCAGTGAATG GCAAAGGTCA AGGAGACTAC 
AGTAAAATAG AAATATTCCA GACACTGCCA GTCCGTGAGC CAAGTCCCCC TTCCATACAT 
GGACAGCCAA GCAGTGGCAA GAGTTTTAAA ATCAGCATCA CCAAGCAAGA TGATGGAGGG 
GCTCCTATTT TAGAATACAT TGTAAAATAC AGAAGTAAAG ACAAGGAAGA TCAGTGGCTA 
GAGAAGAAGG TACAGGGAAA TAAAGACCAC ATTATCTTGG AGCATCTGCA GTGGACAATG 
GGCTACGAAG TGCAAATCAC AGCTGCCAAC AGACTGGGAT ATTCTGAGCC TACTGTATAT 
GAGTTCAGCA TGCCCCCAAA ACCCAACATT ATTAAAGATA ATTGCTGTGA AGCGAATAAA 
GGTGAAAATG GAGGCCAGTC ATGGCATCTG AATGCTGTTG GGTTTACTTT TGTAATAACC 
ATGAGTTTGT CTTGTTTGTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tag1 deficiency results in olfactory dysfunction through impaired migration of mitral cells.
Development (Cambridge, England)142(24)4318-28(2015 Dec)
Bastakis GG,Savvaki M,Stamatakis A,Vidaki M,Karagogeos D

A?-dependent reduction of NCAM2-mediated synaptic adhesion contributes to synapse loss in Alzheimer's disease.
Nature communications68836(2015 Nov)
Leshchyns'ka I,Liew HT,Shepherd C,Halliday GM,Stevens CH,Ke YD,Ittner LM,Sytnyk V

OCAM regulates embryonic spinal cord stem cell proliferation by modulating ErbB2 receptor.
PloS one10(4)e0122337(2015)
Deleyrolle L,Sabourin JC,Rothhut B,Fujita H,Guichet PO,Teigell M,Ripoll C,Chauvet N,Perrin F,Mamaeva D,Noda T,Mori K,Yoshihara Y,Hugnot JP

Neural cell adhesion molecule 2 promotes the formation of filopodia and neurite branching by inducing submembrane increases in Ca2+ levels.
The Journal of neuroscience : the official journal of the Society for Neuroscience35(4)1739-52(2015 Jan)
Sheng L,Leshchyns'ka I,Sytnyk V

Expression of the immunoglobulin superfamily cell adhesion molecules in the developing spinal cord and dorsal root ganglion.
PloS one10(3)e0121550(2015)
Gu Z,Imai F,Kim IJ,Fujita H,Katayama Ki,Mori K,Yoshihara Y,Yoshida Y