Man2a1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Man2a1
Entrez Gene ID	17158
Full Name	mannosidase 2, alpha 1
Synonyms	Mana-2,Mana2,Map-2
General protein information	Preferred Names mannosidase 2, alpha 1 Names alpha-mannosidase 2 AMan II golgi alpha-mannosidase II man II mannosidase alpha class 2A member 1 mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 E1.1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006523739.2	XP_006523802.1	alpha-mannosidase 2 isoform X1
NM_008549.2	NP_032575.2	alpha-mannosidase 2
XM_030249522.1	XP_030105382.1	alpha-mannosidase 2 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MAN2A1	XP_517864.2
Canis lupus familiaris (dog)	MAN2A1	XP_545995.3
Rattus norvegicus (Norway rat)	Man2a1	NP_037111.2
Xenopus tropicalis (tropical clawed frog)	man2a1	NP_001163963.1
Homo sapiens (human)	MAN2A1	NP_002363.2
Gallus gallus (chicken)	MAN2A1	XP_003643094.1
	MAN2A1	XP_002804520.1
Bos taurus (cattle)	MAN2A1	NP_001192815.1
Mus musculus (house mouse)	Man2a1	NP_032575.2

Gene Ontology
Bibliography

Related articles in PubMed

Testosterone deficiency accompanied by testicular and epididymal abnormalities in TMF(-/-) mice.
Elkis Y, Bel S, Lerer-Goldstein T, Nyska A, Creasy DM, Shpungin S, Nir U
Molecular and cellular endocrinology365(1)52-63(2013 Jan)

Pilt is a coiled-coil domain-containing protein that localizes at the trans-Golgi complex and regulates its structure.
Tamaki H, Sanda M, Katsumata O, Hara Y, Fukaya M, Sakagami H
FEBS letters586(19)3064-70(2012 Sep)

Mannosidase 2, alpha 1 deficiency is associated with ricin resistance in embryonic stem (ES) cells.
Wang W, Hale C, Goulding D, Haslam SM, Tissot B, Lindsay C, Michell S, Titball R, Yu J, Toribio AL, Rossi R, Dell A, Bradley A, Dougan G
PloS one6(8)e22993(2011)

Neurog2 controls the leading edge of neurogenesis in the mammalian retina.
Hufnagel RB, Le TT, Riesenberg AL, Brown NL
Developmental biology340(2)490-503(2010 Apr)

Mice lacking mannose 6-phosphate uncovering enzyme activity have a milder phenotype than mice deficient for N-acetylglucosamine-1-phosphotransferase activity.
Boonen M, Vogel P, Platt KA, Dahms N, Kornfeld S
Molecular biology of the cell20(20)4381-9(2009 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that either alpha-Mannosidase II or IIX can biochemically compensate for the deficiency of the other in vivo, and either of two is required for late embryonic and early postnatal development.
Title: Essential and mutually compensatory roles of {alpha}-mannosidase II and {alpha}-mannosidase IIx in N-glycan processing in vivo in mice.

Soluble MII was prepared by transfecting mammalian cells with expression vectors and determined substrate specificity of MX.
Title: N-Glycan structure analysis using lectins and an alpha-mannosidase activity assay.

The following Man2a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Man2a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu53200	XM_006523739.2 Latest version!	Mus musculus mannosidase 2, alpha 1 (Man2a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu53200	XM_030249522.1 Latest version!	Mus musculus mannosidase 2, alpha 1 (Man2a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu02203	NM_008549.2 Latest version!	Mus musculus mannosidase 2, alpha 1 (Man2a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$678.30 $969.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Man2a1 ( XM_006523739.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523802.1 Mus musculus mannosidase 2, alpha 1 (Man2a1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu53200
Clone ID Related Accession (Same CDS sequence)	XM_006523739.2 , XM_030249522.1
Accession Version	XM_006523739.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	alpha-mannosidase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523739.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATG AAGCCACTCC ACATTATTTT GCCTTAATTG ACCAACTAAT TGAAGGGCAC 
CAATGGCTGG AAAAAAATCT AGGAGTGAAA CCTCGATCGG GCTGGGCCAT AGATCCCTTT 
GGTCATTCAC CCACAATGGC TTATCTTCTA AAGCGTGCTG GATTTTCACA CATGCTCATC 
CAGAGAGTCC ATTATGCAAT CAAAAAACAC TTCTCTTTGC ATAAAACGCT GGAGTTTTTC 
TGGAGACAGA ATTGGGATCT TGGATCTGCT ACAGACATTT TGTGCCATAT GATGCCCTTC 
TACAGCTACG ACATCCCTCA CACCTGTGGG CCTGATCCTA AAATATGCTG CCAGTTTGAT 
TTTAAACGGC TTCCTGGAGG CAGATATGGT TGTCCCTGGG GAGTTCCCCC AGAAGCAATA 
TCTCCTGGAA ATGTCCAAAG CAGGGCTCAG ATGCTATTGG ATCAGTACCG GAAAAAGTCA 
AAACTTTTCC GCACTAAAGT TCTGCTGGCT CCACTGGGAG ACGACTTTCG GTTCAGTGAA 
TACACAGAGT GGGATCTGCA GTGCAGGAAC TACGAGCAAC TGTTCAGTTA CATGAACTCG 
CAGCCTCATC TGAAAGTGAA GATCCAGTTT GGAACCTTGT CAGATTATTT CGACGCATTG 
GAGAAAGCGG TGGCAGCCGA GAAGAAGAGT AGCCAGTCTG TGTTCCCTGC CCTGAGTGGA 
GACTTCTTCA CGTACGCTGA CAGAGACGAC CATTACTGGA GTGGCTACTT CACGTCCAGA 
CCTTTCTACA AACGAATGGA CAGAATAATG GAATCTCGTA TAAGGGCTGC TGAAATTCTT 
TACCAGTTGG CCTTGAAACA AGCTCAGAAA TACAAGATAA ATAAATTTCT TTCATCACCT 
CATTACACAA CACTGACAGA AGCCAGAAGG AACTTAGGAC TATTTCAGCA TCATGATGCC 
ATCACAGGAA CCGCGAAAGA CTGGGTGGTT GTGGACTATG GTACCAGACT CTTTCAGTCA 
TTAAATTCTT TGGAGAAGAT AATTGGAGAT TCTGCATTTC TTCTCATTTT AAAGGACAAA 
AAGCTGTACC AGTCAGATCC TTCCAAAGCC TTCTTAGAGA TGGATACGAA GCAAAGTTCA 
CAAGATTCTC TGCCCCAAAA AATTATAATA CAACTGAGCG CACAGGAGCC AAGGTACCTT 
GTGGTCTACA ATCCCTTTGA ACAAGAACGG CATTCAGTGG TGTCCATCCG GGTAAACTCC 
GCCACAGTGA AAGTGCTGTC TGATTCGGGA AAACCGGTGG AGGTTCAAGT CAGTGCAGTT 
TGGAACGACA TGAGGACAAT TTCACAAGCA GCCTATGAGG TTTCTTTTCT AGCTCATATA 
CCACCACTGG GACTGAAAGT GTTTAAGATC TTAGAGTCAC AAAGTTCAAG CTCACACTTG 
GCTGATTATG TCCTATATAA TAATGATGGA CTAGCAGAAA ATGGAATATT CCACGTGAAG 
AACATGGTGG ATGCTGGAGA TGCCATAACA ATAGAGAATC CCTTCCTGGC GATTTGGTTT 
GACCGATCTG GGCTGATGGA GAAAGTGAGA AGGAAAGAAG ACAGTAGACA GCATGAACTG 
AAGGTCCAGT TCCTGTGGTA CGGAACCACC AACAAAAGGG ACAAGAGCGG TGCCTACCTC 
TTCCTGCCTG ACGGGCAGGG CCAGCCATAT GTTTCCCTAA GACCGCCCTT TGTCAGAGTG 
ACACGTGGAA GGATCTACTC AGATGTGACC TGTTTCCTCG AACACGTTAC TCACAAAGTC 
CGCCTGTACA ACATTCAGGG AATAGAAGGT CAGTCCATGG AAGTTTCTAA TATTGTAAAC 
ATCAGGAATG TGCATAACCG TGAGATTGTA ATGAGAATTT CATCTAAAAT AAACAACCAA 
AATAGATATT ATACTGACCT AAATGGATAT CAGATTCAGC CTAGAAGGAC CATGAGCAAA 
TTGCCTCTTC AAGCCAACGT TTACCCGATG TGCACAATGG CGTATATCCA GGATGCTGAG 
CACCGGCTCA CGCTGCTCTC TGCTCAGTCT CTAGGTGCTT CCAGCATGGC TTCTGGTCAG 
ATTGAAGTCT TCATGGATCG AAGGCTCATG CAGGATGATA ACCGTGGCCT TGGGCAAGGC 
GTCCATGACA ATAAGATTAC AGCTAATTTG TTTCGAATCC TCCTCGAGAA GAGAAGCGCT 
GTGAACATGG AAGAAGAAAA GAAGAGCCCT GTCAGCTACC CTTCCCTCCT CAGCCACATG 
ACTTCGTCCT TCCTCAACCA TCCCTTTCTC CCCATGGTAC TAAGTGGCCA GCTCCCCTCC 
CCTGCCTTTG AGCTGCTGAG TGAATTTCCT CTGCTGCAGT CCTCTCTACC TTGTGATATC 
CATCTGGTCA ACCTGCGGAC AATACAATCA AAGATGGGCA AAGGCTATTC GGATGAGGCA 
GCCTTGATCC TCCACAGGAA AGGGTTTGAT TGCCAGTTCT CCAGCAGAGG CATCGGGCTA 
CCCTGTTCCA CTACTCAGGG AAAGATGTCA GTTCTGAAAC TTTTCAACAA GTTTGCTGTG 
GAGAGTCTCG TCCCTTCCTC TCTGTCCTTG ATGCACTCCC CTCCAGATGC CCAGAACATG 
AGTGAAGTCA GCCTGAGCCC CATGGAGATC AGCACGTTCC GTATCCGCTT GCGTTGGACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523802.1
CDS	127..2829
Translation	MPDEATPHYFALIDQLIEGHQWLEKNLGVKPRSGWAIDPFGHSPTMAYLLKRAGFSHMLIQRVHYAIKKHFSLHKTLEFFWRQNWDLGSATDILCHMMPFYSYDIPHTCGPDPKICCQFDFKRLPGGRYGCPWGVPPEAISPGNVQSRAQMLLDQYRKKSKLFRTKVLLAPLGDDFRFSEYTEWDLQCRNYEQLFSYMNSQPHLKVKIQFGTLSDYFDALEKAVAAEKKSSQSVFPALSGDFFTYADRDDHYWSGYFTSRPFYKRMDRIMESRIRAAEILYQLALKQAQKYKINKFLSSPHYTTLTEARRNLGLFQHHDAITGTAKDWVVVDYGTRLFQSLNSLEKIIGDSAFLLILKDKKLYQSDPSKAFLEMDTKQSSQDSLPQKIIIQLSAQEPRYLVVYNPFEQERHSVVSIRVNSATVKVLSDSGKPVEVQVSAVWNDMRTISQAAYEVSFLAHIPPLGLKVFKILESQSSSSHLADYVLYNNDGLAENGIFHVKNMVDAGDAITIENPFLAIWFDRSGLMEKVRRKEDSRQHELKVQFLWYGTTNKRDKSGAYLFLPDGQGQPYVSLRPPFVRVTRGRIYSDVTCFLEHVTHKVRLYNIQGIEGQSMEVSNIVNIRNVHNREIVMRISSKINNQNRYYTDLNGYQIQPRRTMSKLPLQANVYPMCTMAYIQDAEHRLTLLSAQSLGASSMASGQIEVFMDRRLMQDDNRGLGQGVHDNKITANLFRILLEKRSAVNMEEEKKSPVSYPSLLSHMTSSFLNHPFLPMVLSGQLPSPAFELLSEFPLLQSSLPCDIHLVNLRTIQSKMGKGYSDEAALILHRKGFDCQFSSRGIGLPCSTTQGKMSVLKLFNKFAVESLVPSSLSLMHSPPDAQNMSEVSLSPMEISTFRIRLRWT

Target ORF information:

RefSeq Version	XM_006523739.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mannosidase 2, alpha 1 (Man2a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523739.2

ORF Insert Sequence:

ATGCCTGATG AAGCCACTCC ACATTATTTT GCCTTAATTG ACCAACTAAT TGAAGGGCAC 
CAATGGCTGG AAAAAAATCT AGGAGTGAAA CCTCGATCGG GCTGGGCCAT AGATCCCTTT 
GGTCATTCAC CCACAATGGC TTATCTTCTA AAGCGTGCTG GATTTTCACA CATGCTCATC 
CAGAGAGTCC ATTATGCAAT CAAAAAACAC TTCTCTTTGC ATAAAACGCT GGAGTTTTTC 
TGGAGACAGA ATTGGGATCT TGGATCTGCT ACAGACATTT TGTGCCATAT GATGCCCTTC 
TACAGCTACG ACATCCCTCA CACCTGTGGG CCTGATCCTA AAATATGCTG CCAGTTTGAT 
TTTAAACGGC TTCCTGGAGG CAGATATGGT TGTCCCTGGG GAGTTCCCCC AGAAGCAATA 
TCTCCTGGAA ATGTCCAAAG CAGGGCTCAG ATGCTATTGG ATCAGTACCG GAAAAAGTCA 
AAACTTTTCC GCACTAAAGT TCTGCTGGCT CCACTGGGAG ACGACTTTCG GTTCAGTGAA 
TACACAGAGT GGGATCTGCA GTGCAGGAAC TACGAGCAAC TGTTCAGTTA CATGAACTCG 
CAGCCTCATC TGAAAGTGAA GATCCAGTTT GGAACCTTGT CAGATTATTT CGACGCATTG 
GAGAAAGCGG TGGCAGCCGA GAAGAAGAGT AGCCAGTCTG TGTTCCCTGC CCTGAGTGGA 
GACTTCTTCA CGTACGCTGA CAGAGACGAC CATTACTGGA GTGGCTACTT CACGTCCAGA 
CCTTTCTACA AACGAATGGA CAGAATAATG GAATCTCGTA TAAGGGCTGC TGAAATTCTT 
TACCAGTTGG CCTTGAAACA AGCTCAGAAA TACAAGATAA ATAAATTTCT TTCATCACCT 
CATTACACAA CACTGACAGA AGCCAGAAGG AACTTAGGAC TATTTCAGCA TCATGATGCC 
ATCACAGGAA CCGCGAAAGA CTGGGTGGTT GTGGACTATG GTACCAGACT CTTTCAGTCA 
TTAAATTCTT TGGAGAAGAT AATTGGAGAT TCTGCATTTC TTCTCATTTT AAAGGACAAA 
AAGCTGTACC AGTCAGATCC TTCCAAAGCC TTCTTAGAGA TGGATACGAA GCAAAGTTCA 
CAAGATTCTC TGCCCCAAAA AATTATAATA CAACTGAGCG CACAGGAGCC AAGGTACCTT 
GTGGTCTACA ATCCCTTTGA ACAAGAACGG CATTCAGTGG TGTCCATCCG GGTAAACTCC 
GCCACAGTGA AAGTGCTGTC TGATTCGGGA AAACCGGTGG AGGTTCAAGT CAGTGCAGTT 
TGGAACGACA TGAGGACAAT TTCACAAGCA GCCTATGAGG TTTCTTTTCT AGCTCATATA 
CCACCACTGG GACTGAAAGT GTTTAAGATC TTAGAGTCAC AAAGTTCAAG CTCACACTTG 
GCTGATTATG TCCTATATAA TAATGATGGA CTAGCAGAAA ATGGAATATT CCACGTGAAG 
AACATGGTGG ATGCTGGAGA TGCCATAACA ATAGAGAATC CCTTCCTGGC GATTTGGTTT 
GACCGATCTG GGCTGATGGA GAAAGTGAGA AGGAAAGAAG ACAGTAGACA GCATGAACTG 
AAGGTCCAGT TCCTGTGGTA CGGAACCACC AACAAAAGGG ACAAGAGCGG TGCCTACCTC 
TTCCTGCCTG ACGGGCAGGG CCAGCCATAT GTTTCCCTAA GACCGCCCTT TGTCAGAGTG 
ACACGTGGAA GGATCTACTC AGATGTGACC TGTTTCCTCG AACACGTTAC TCACAAAGTC 
CGCCTGTACA ACATTCAGGG AATAGAAGGT CAGTCCATGG AAGTTTCTAA TATTGTAAAC 
ATCAGGAATG TGCATAACCG TGAGATTGTA ATGAGAATTT CATCTAAAAT AAACAACCAA 
AATAGATATT ATACTGACCT AAATGGATAT CAGATTCAGC CTAGAAGGAC CATGAGCAAA 
TTGCCTCTTC AAGCCAACGT TTACCCGATG TGCACAATGG CGTATATCCA GGATGCTGAG 
CACCGGCTCA CGCTGCTCTC TGCTCAGTCT CTAGGTGCTT CCAGCATGGC TTCTGGTCAG 
ATTGAAGTCT TCATGGATCG AAGGCTCATG CAGGATGATA ACCGTGGCCT TGGGCAAGGC 
GTCCATGACA ATAAGATTAC AGCTAATTTG TTTCGAATCC TCCTCGAGAA GAGAAGCGCT 
GTGAACATGG AAGAAGAAAA GAAGAGCCCT GTCAGCTACC CTTCCCTCCT CAGCCACATG 
ACTTCGTCCT TCCTCAACCA TCCCTTTCTC CCCATGGTAC TAAGTGGCCA GCTCCCCTCC 
CCTGCCTTTG AGCTGCTGAG TGAATTTCCT CTGCTGCAGT CCTCTCTACC TTGTGATATC 
CATCTGGTCA ACCTGCGGAC AATACAATCA AAGATGGGCA AAGGCTATTC GGATGAGGCA 
GCCTTGATCC TCCACAGGAA AGGGTTTGAT TGCCAGTTCT CCAGCAGAGG CATCGGGCTA 
CCCTGTTCCA CTACTCAGGG AAAGATGTCA GTTCTGAAAC TTTTCAACAA GTTTGCTGTG 
GAGAGTCTCG TCCCTTCCTC TCTGTCCTTG ATGCACTCCC CTCCAGATGC CCAGAACATG 
AGTGAAGTCA GCCTGAGCCC CATGGAGATC AGCACGTTCC GTATCCGCTT GCGTTGGACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Man2a1 ( XM_030249522.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105382.1 Mus musculus mannosidase 2, alpha 1 (Man2a1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu53200
Clone ID Related Accession (Same CDS sequence)	XM_006523739.2 , XM_030249522.1
Accession Version	XM_030249522.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	alpha-mannosidase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATG AAGCCACTCC ACATTATTTT GCCTTAATTG ACCAACTAAT TGAAGGGCAC 
CAATGGCTGG AAAAAAATCT AGGAGTGAAA CCTCGATCGG GCTGGGCCAT AGATCCCTTT 
GGTCATTCAC CCACAATGGC TTATCTTCTA AAGCGTGCTG GATTTTCACA CATGCTCATC 
CAGAGAGTCC ATTATGCAAT CAAAAAACAC TTCTCTTTGC ATAAAACGCT GGAGTTTTTC 
TGGAGACAGA ATTGGGATCT TGGATCTGCT ACAGACATTT TGTGCCATAT GATGCCCTTC 
TACAGCTACG ACATCCCTCA CACCTGTGGG CCTGATCCTA AAATATGCTG CCAGTTTGAT 
TTTAAACGGC TTCCTGGAGG CAGATATGGT TGTCCCTGGG GAGTTCCCCC AGAAGCAATA 
TCTCCTGGAA ATGTCCAAAG CAGGGCTCAG ATGCTATTGG ATCAGTACCG GAAAAAGTCA 
AAACTTTTCC GCACTAAAGT TCTGCTGGCT CCACTGGGAG ACGACTTTCG GTTCAGTGAA 
TACACAGAGT GGGATCTGCA GTGCAGGAAC TACGAGCAAC TGTTCAGTTA CATGAACTCG 
CAGCCTCATC TGAAAGTGAA GATCCAGTTT GGAACCTTGT CAGATTATTT CGACGCATTG 
GAGAAAGCGG TGGCAGCCGA GAAGAAGAGT AGCCAGTCTG TGTTCCCTGC CCTGAGTGGA 
GACTTCTTCA CGTACGCTGA CAGAGACGAC CATTACTGGA GTGGCTACTT CACGTCCAGA 
CCTTTCTACA AACGAATGGA CAGAATAATG GAATCTCGTA TAAGGGCTGC TGAAATTCTT 
TACCAGTTGG CCTTGAAACA AGCTCAGAAA TACAAGATAA ATAAATTTCT TTCATCACCT 
CATTACACAA CACTGACAGA AGCCAGAAGG AACTTAGGAC TATTTCAGCA TCATGATGCC 
ATCACAGGAA CCGCGAAAGA CTGGGTGGTT GTGGACTATG GTACCAGACT CTTTCAGTCA 
TTAAATTCTT TGGAGAAGAT AATTGGAGAT TCTGCATTTC TTCTCATTTT AAAGGACAAA 
AAGCTGTACC AGTCAGATCC TTCCAAAGCC TTCTTAGAGA TGGATACGAA GCAAAGTTCA 
CAAGATTCTC TGCCCCAAAA AATTATAATA CAACTGAGCG CACAGGAGCC AAGGTACCTT 
GTGGTCTACA ATCCCTTTGA ACAAGAACGG CATTCAGTGG TGTCCATCCG GGTAAACTCC 
GCCACAGTGA AAGTGCTGTC TGATTCGGGA AAACCGGTGG AGGTTCAAGT CAGTGCAGTT 
TGGAACGACA TGAGGACAAT TTCACAAGCA GCCTATGAGG TTTCTTTTCT AGCTCATATA 
CCACCACTGG GACTGAAAGT GTTTAAGATC TTAGAGTCAC AAAGTTCAAG CTCACACTTG 
GCTGATTATG TCCTATATAA TAATGATGGA CTAGCAGAAA ATGGAATATT CCACGTGAAG 
AACATGGTGG ATGCTGGAGA TGCCATAACA ATAGAGAATC CCTTCCTGGC GATTTGGTTT 
GACCGATCTG GGCTGATGGA GAAAGTGAGA AGGAAAGAAG ACAGTAGACA GCATGAACTG 
AAGGTCCAGT TCCTGTGGTA CGGAACCACC AACAAAAGGG ACAAGAGCGG TGCCTACCTC 
TTCCTGCCTG ACGGGCAGGG CCAGCCATAT GTTTCCCTAA GACCGCCCTT TGTCAGAGTG 
ACACGTGGAA GGATCTACTC AGATGTGACC TGTTTCCTCG AACACGTTAC TCACAAAGTC 
CGCCTGTACA ACATTCAGGG AATAGAAGGT CAGTCCATGG AAGTTTCTAA TATTGTAAAC 
ATCAGGAATG TGCATAACCG TGAGATTGTA ATGAGAATTT CATCTAAAAT AAACAACCAA 
AATAGATATT ATACTGACCT AAATGGATAT CAGATTCAGC CTAGAAGGAC CATGAGCAAA 
TTGCCTCTTC AAGCCAACGT TTACCCGATG TGCACAATGG CGTATATCCA GGATGCTGAG 
CACCGGCTCA CGCTGCTCTC TGCTCAGTCT CTAGGTGCTT CCAGCATGGC TTCTGGTCAG 
ATTGAAGTCT TCATGGATCG AAGGCTCATG CAGGATGATA ACCGTGGCCT TGGGCAAGGC 
GTCCATGACA ATAAGATTAC AGCTAATTTG TTTCGAATCC TCCTCGAGAA GAGAAGCGCT 
GTGAACATGG AAGAAGAAAA GAAGAGCCCT GTCAGCTACC CTTCCCTCCT CAGCCACATG 
ACTTCGTCCT TCCTCAACCA TCCCTTTCTC CCCATGGTAC TAAGTGGCCA GCTCCCCTCC 
CCTGCCTTTG AGCTGCTGAG TGAATTTCCT CTGCTGCAGT CCTCTCTACC TTGTGATATC 
CATCTGGTCA ACCTGCGGAC AATACAATCA AAGATGGGCA AAGGCTATTC GGATGAGGCA 
GCCTTGATCC TCCACAGGAA AGGGTTTGAT TGCCAGTTCT CCAGCAGAGG CATCGGGCTA 
CCCTGTTCCA CTACTCAGGG AAAGATGTCA GTTCTGAAAC TTTTCAACAA GTTTGCTGTG 
GAGAGTCTCG TCCCTTCCTC TCTGTCCTTG ATGCACTCCC CTCCAGATGC CCAGAACATG 
AGTGAAGTCA GCCTGAGCCC CATGGAGATC AGCACGTTCC GTATCCGCTT GCGTTGGACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105382.1
CDS	1627..4329
Translation	MPDEATPHYFALIDQLIEGHQWLEKNLGVKPRSGWAIDPFGHSPTMAYLLKRAGFSHMLIQRVHYAIKKHFSLHKTLEFFWRQNWDLGSATDILCHMMPFYSYDIPHTCGPDPKICCQFDFKRLPGGRYGCPWGVPPEAISPGNVQSRAQMLLDQYRKKSKLFRTKVLLAPLGDDFRFSEYTEWDLQCRNYEQLFSYMNSQPHLKVKIQFGTLSDYFDALEKAVAAEKKSSQSVFPALSGDFFTYADRDDHYWSGYFTSRPFYKRMDRIMESRIRAAEILYQLALKQAQKYKINKFLSSPHYTTLTEARRNLGLFQHHDAITGTAKDWVVVDYGTRLFQSLNSLEKIIGDSAFLLILKDKKLYQSDPSKAFLEMDTKQSSQDSLPQKIIIQLSAQEPRYLVVYNPFEQERHSVVSIRVNSATVKVLSDSGKPVEVQVSAVWNDMRTISQAAYEVSFLAHIPPLGLKVFKILESQSSSSHLADYVLYNNDGLAENGIFHVKNMVDAGDAITIENPFLAIWFDRSGLMEKVRRKEDSRQHELKVQFLWYGTTNKRDKSGAYLFLPDGQGQPYVSLRPPFVRVTRGRIYSDVTCFLEHVTHKVRLYNIQGIEGQSMEVSNIVNIRNVHNREIVMRISSKINNQNRYYTDLNGYQIQPRRTMSKLPLQANVYPMCTMAYIQDAEHRLTLLSAQSLGASSMASGQIEVFMDRRLMQDDNRGLGQGVHDNKITANLFRILLEKRSAVNMEEEKKSPVSYPSLLSHMTSSFLNHPFLPMVLSGQLPSPAFELLSEFPLLQSSLPCDIHLVNLRTIQSKMGKGYSDEAALILHRKGFDCQFSSRGIGLPCSTTQGKMSVLKLFNKFAVESLVPSSLSLMHSPPDAQNMSEVSLSPMEISTFRIRLRWT

Target ORF information:

RefSeq Version	XM_030249522.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mannosidase 2, alpha 1 (Man2a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249522.1

ORF Insert Sequence:

ATGCCTGATG AAGCCACTCC ACATTATTTT GCCTTAATTG ACCAACTAAT TGAAGGGCAC 
CAATGGCTGG AAAAAAATCT AGGAGTGAAA CCTCGATCGG GCTGGGCCAT AGATCCCTTT 
GGTCATTCAC CCACAATGGC TTATCTTCTA AAGCGTGCTG GATTTTCACA CATGCTCATC 
CAGAGAGTCC ATTATGCAAT CAAAAAACAC TTCTCTTTGC ATAAAACGCT GGAGTTTTTC 
TGGAGACAGA ATTGGGATCT TGGATCTGCT ACAGACATTT TGTGCCATAT GATGCCCTTC 
TACAGCTACG ACATCCCTCA CACCTGTGGG CCTGATCCTA AAATATGCTG CCAGTTTGAT 
TTTAAACGGC TTCCTGGAGG CAGATATGGT TGTCCCTGGG GAGTTCCCCC AGAAGCAATA 
TCTCCTGGAA ATGTCCAAAG CAGGGCTCAG ATGCTATTGG ATCAGTACCG GAAAAAGTCA 
AAACTTTTCC GCACTAAAGT TCTGCTGGCT CCACTGGGAG ACGACTTTCG GTTCAGTGAA 
TACACAGAGT GGGATCTGCA GTGCAGGAAC TACGAGCAAC TGTTCAGTTA CATGAACTCG 
CAGCCTCATC TGAAAGTGAA GATCCAGTTT GGAACCTTGT CAGATTATTT CGACGCATTG 
GAGAAAGCGG TGGCAGCCGA GAAGAAGAGT AGCCAGTCTG TGTTCCCTGC CCTGAGTGGA 
GACTTCTTCA CGTACGCTGA CAGAGACGAC CATTACTGGA GTGGCTACTT CACGTCCAGA 
CCTTTCTACA AACGAATGGA CAGAATAATG GAATCTCGTA TAAGGGCTGC TGAAATTCTT 
TACCAGTTGG CCTTGAAACA AGCTCAGAAA TACAAGATAA ATAAATTTCT TTCATCACCT 
CATTACACAA CACTGACAGA AGCCAGAAGG AACTTAGGAC TATTTCAGCA TCATGATGCC 
ATCACAGGAA CCGCGAAAGA CTGGGTGGTT GTGGACTATG GTACCAGACT CTTTCAGTCA 
TTAAATTCTT TGGAGAAGAT AATTGGAGAT TCTGCATTTC TTCTCATTTT AAAGGACAAA 
AAGCTGTACC AGTCAGATCC TTCCAAAGCC TTCTTAGAGA TGGATACGAA GCAAAGTTCA 
CAAGATTCTC TGCCCCAAAA AATTATAATA CAACTGAGCG CACAGGAGCC AAGGTACCTT 
GTGGTCTACA ATCCCTTTGA ACAAGAACGG CATTCAGTGG TGTCCATCCG GGTAAACTCC 
GCCACAGTGA AAGTGCTGTC TGATTCGGGA AAACCGGTGG AGGTTCAAGT CAGTGCAGTT 
TGGAACGACA TGAGGACAAT TTCACAAGCA GCCTATGAGG TTTCTTTTCT AGCTCATATA 
CCACCACTGG GACTGAAAGT GTTTAAGATC TTAGAGTCAC AAAGTTCAAG CTCACACTTG 
GCTGATTATG TCCTATATAA TAATGATGGA CTAGCAGAAA ATGGAATATT CCACGTGAAG 
AACATGGTGG ATGCTGGAGA TGCCATAACA ATAGAGAATC CCTTCCTGGC GATTTGGTTT 
GACCGATCTG GGCTGATGGA GAAAGTGAGA AGGAAAGAAG ACAGTAGACA GCATGAACTG 
AAGGTCCAGT TCCTGTGGTA CGGAACCACC AACAAAAGGG ACAAGAGCGG TGCCTACCTC 
TTCCTGCCTG ACGGGCAGGG CCAGCCATAT GTTTCCCTAA GACCGCCCTT TGTCAGAGTG 
ACACGTGGAA GGATCTACTC AGATGTGACC TGTTTCCTCG AACACGTTAC TCACAAAGTC 
CGCCTGTACA ACATTCAGGG AATAGAAGGT CAGTCCATGG AAGTTTCTAA TATTGTAAAC 
ATCAGGAATG TGCATAACCG TGAGATTGTA ATGAGAATTT CATCTAAAAT AAACAACCAA 
AATAGATATT ATACTGACCT AAATGGATAT CAGATTCAGC CTAGAAGGAC CATGAGCAAA 
TTGCCTCTTC AAGCCAACGT TTACCCGATG TGCACAATGG CGTATATCCA GGATGCTGAG 
CACCGGCTCA CGCTGCTCTC TGCTCAGTCT CTAGGTGCTT CCAGCATGGC TTCTGGTCAG 
ATTGAAGTCT TCATGGATCG AAGGCTCATG CAGGATGATA ACCGTGGCCT TGGGCAAGGC 
GTCCATGACA ATAAGATTAC AGCTAATTTG TTTCGAATCC TCCTCGAGAA GAGAAGCGCT 
GTGAACATGG AAGAAGAAAA GAAGAGCCCT GTCAGCTACC CTTCCCTCCT CAGCCACATG 
ACTTCGTCCT TCCTCAACCA TCCCTTTCTC CCCATGGTAC TAAGTGGCCA GCTCCCCTCC 
CCTGCCTTTG AGCTGCTGAG TGAATTTCCT CTGCTGCAGT CCTCTCTACC TTGTGATATC 
CATCTGGTCA ACCTGCGGAC AATACAATCA AAGATGGGCA AAGGCTATTC GGATGAGGCA 
GCCTTGATCC TCCACAGGAA AGGGTTTGAT TGCCAGTTCT CCAGCAGAGG CATCGGGCTA 
CCCTGTTCCA CTACTCAGGG AAAGATGTCA GTTCTGAAAC TTTTCAACAA GTTTGCTGTG 
GAGAGTCTCG TCCCTTCCTC TCTGTCCTTG ATGCACTCCC CTCCAGATGC CCAGAACATG 
AGTGAAGTCA GCCTGAGCCC CATGGAGATC AGCACGTTCC GTATCCGCTT GCGTTGGACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Man2a1 ( NM_008549.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032575.2 Mus musculus mannosidase 2, alpha 1 (Man2a1), mRNA.

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CloneID	OMu02203
Clone ID Related Accession (Same CDS sequence)	NM_008549.2
Accession Version	NM_008549.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	alpha-mannosidase 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK144763.1, BC138372.1, BC138371.1, AK081775.1 and AI461855.1. On Apr 2, 2009 this sequence version replaced NM_008549.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X61172.1, BC138372.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGTTAA GTCGCCAGTT CACCGTGTTT GGCAGCGCGA TCTTCTGCGT CGTAATCTTC 
TCACTCTACC TGATGCTGGA CAGGGGTCAC TTGGACTACC CTCGGGGCCC GCGCCAGGAG 
GGCTCCTTTC CGCAGGGCCA GCTTTCAATA TTGCAAGAAA AGATTGACCA TTTGGAGCGT 
TTGCTCGCTG AGAACAACGA GATCATCTCA AATATCAGAG ACTCAGTCAT CAACCTGAGC 
GAGTCTGTGG AGGACGGCCC GCGGGGGTCA CCAGGCAACG CCAGCCAAGG CTCCATCCAC 
CTCCACTCGC CACAGTTGGC CCTGCAGGCT GACCCCAGAG ACTGTTTGTT TGCTTCACAG 
AGTGGGAGTC AGCCCCGGGA TGTGCAGATG TTGGATGTTT ACGATCTGAT TCCTTTTGAT 
AATCCAGATG GTGGAGTTTG GAAGCAAGGA TTTGACATTA AGTATGAAGC GGATGAGTGG 
GACCATGAGC CCCTGCAAGT GTTTGTGGTG CCTCACTCCC ATAATGACCC AGGTTGGTTG 
AAGACTTTCA ATGACTACTT TAGAGACAAG ACTCAGTATA TTTTTAATAA CATGGTCCTA 
AAGCTGAAAG AAGACTCAAG CAGGAAGTTT ATGTGGTCTG AGATCTCTTA CCTTGCAAAA 
TGGTGGGATA TTATAGATAT TCCGAAGAAG GAAGCTGTTA AAAGTTTACT ACAGAATGGT 
CAGCTGGAAA TTGTGACCGG TGGCTGGGTT ATGCCTGATG AAGCCACTCC ACATTATTTT 
GCCTTAATTG ACCAACTAAT TGAAGGGCAC CAATGGCTGG AAAAAAATCT AGGAGTGAAA 
CCTCGATCGG GCTGGGCCAT AGATCCCTTT GGTCATTCAC CCACAATGGC TTATCTTCTA 
AAGCGTGCTG GATTTTCACA CATGCTCATC CAGAGAGTCC ATTATGCAAT CAAAAAACAC 
TTCTCTTTGC ATAAAACGCT GGAGTTTTTC TGGAGACAGA ATTGGGATCT TGGATCTGCT 
ACAGACATTT TGTGCCATAT GATGCCCTTC TACAGCTACG ACATCCCTCA CACCTGTGGG 
CCTGATCCTA AAATATGCTG CCAGTTTGAT TTTAAACGGC TTCCTGGAGG CAGATATGGT 
TGTCCCTGGG GAGTTCCCCC AGAAGCAATA TCTCCTGGAA ATGTCCAAAG CAGGGCTCAG 
ATGCTATTGG ATCAGTACCG GAAAAAGTCA AAACTTTTCC GCACTAAAGT TCTGCTGGCT 
CCACTGGGAG ACGACTTTCG GTTCAGTGAA TACACAGAGT GGGATCTGCA GTGCAGGAAC 
TACGAGCAAC TGTTCAGTTA CATGAACTCG CAGCCTCATC TGAAAGTGAA GATCCAGTTT 
GGAACCTTGT CAGATTATTT CGACGCATTG GAGAAAGCGG TGGCAGCCGA GAAGAAGAGT 
AGCCAGTCTG TGTTCCCTGC CCTGAGTGGA GACTTCTTCA CGTACGCTGA CAGAGACGAC 
CATTACTGGA GTGGCTACTT CACGTCCAGA CCTTTCTACA AACGAATGGA CAGAATAATG 
GAATCTCGTA TAAGGGCTGC TGAAATTCTT TACCAGTTGG CCTTGAAACA AGCTCAGAAA 
TACAAGATAA ATAAATTTCT TTCATCACCT CATTACACAA CACTGACAGA AGCCAGAAGG 
AACTTAGGAC TATTTCAGCA TCATGATGCC ATCACAGGAA CCGCGAAAGA CTGGGTGGTT 
GTGGACTATG GTACCAGACT CTTTCAGTCA TTAAATTCTT TGGAGAAGAT AATTGGAGAT 
TCTGCATTTC TTCTCATTTT AAAGGACAAA AAGCTGTACC AGTCAGATCC TTCCAAAGCC 
TTCTTAGAGA TGGATACGAA GCAAAGTTCA CAAGATTCTC TGCCCCAAAA AATTATAATA 
CAACTGAGCG CACAGGAGCC AAGGTACCTT GTGGTCTACA ATCCCTTTGA ACAAGAACGG 
CATTCAGTGG TGTCCATCCG GGTAAACTCC GCCACAGTGA AAGTGCTGTC TGATTCGGGA 
AAACCGGTGG AGGTTCAAGT CAGTGCAGTT TGGAACGACA TGAGGACAAT TTCACAAGCA 
GCCTATGAGG TTTCTTTTCT AGCTCATATA CCACCACTGG GACTGAAAGT GTTTAAGATC 
TTAGAGTCAC AAAGTTCAAG CTCACACTTG GCTGATTATG TCCTATATAA TAATGATGGA 
CTAGCAGAAA ATGGAATATT CCACGTGAAG AACATGGTGG ATGCTGGAGA TGCCATAACA 
ATAGAGAATC CCTTCCTGGC GATTTGGTTT GACCGATCTG GGCTGATGGA GAAAGTGAGA 
AGGAAAGAAG ACAGTAGACA GCATGAACTG AAGGTCCAGT TCCTGTGGTA CGGAACCACC 
AACAAAAGGG ACAAGAGCGG TGCCTACCTC TTCCTGCCTG ACGGGCAGGG CCAGCCATAT 
GTTTCCCTAA GACCGCCCTT TGTCAGAGTG ACACGTGGAA GGATCTACTC AGATGTGACC 
TGTTTCCTCG AACACGTTAC TCACAAAGTC CGCCTGTACA ACATTCAGGG AATAGAAGGT 
CAGTCCATGG AAGTTTCTAA TATTGTAAAC ATCAGGAATG TGCATAACCG TGAGATTGTA 
ATGAGAATTT CATCTAAAAT AAACAACCAA AATAGATATT ATACTGACCT AAATGGATAT 
CAGATTCAGC CTAGAAGGAC CATGAGCAAA TTGCCTCTTC AAGCCAACGT TTACCCGATG 
TGCACAATGG CGTATATCCA GGATGCTGAG CACCGGCTCA CGCTGCTCTC TGCTCAGTCT 
CTAGGTGCTT CCAGCATGGC TTCTGGTCAG ATTGAAGTCT TCATGGATCG AAGGCTCATG 
CAGGATGATA ACCGTGGCCT TGGGCAAGGC GTCCATGACA ATAAGATTAC AGCTAATTTG 
TTTCGAATCC TCCTCGAGAA GAGAAGCGCT GTGAACATGG AAGAAGAAAA GAAGAGCCCT 
GTCAGCTACC CTTCCCTCCT CAGCCACATG ACTTCGTCCT TCCTCAACCA TCCCTTTCTC 
CCCATGGTAC TAAGTGGCCA GCTCCCCTCC CCTGCCTTTG AGCTGCTGAG TGAATTTCCT 
CTGCTGCAGT CCTCTCTACC TTGTGATATC CATCTGGTCA ACCTGCGGAC AATACAATCA 
AAGATGGGCA AAGGCTATTC GGATGAGGCA GCCTTGATCC TCCACAGGAA AGGGTTTGAT 
TGCCAGTTCT CCAGCAGAGG CATCGGGCTA CCCTGTTCCA CTACTCAGGG AAAGATGTCA 
GTTCTGAAAC TTTTCAACAA GTTTGCTGTG GAGAGTCTCG TCCCTTCCTC TCTGTCCTTG 
ATGCACTCCC CTCCAGATGC CCAGAACATG AGTGAAGTCA GCCTGAGCCC CATGGAGATC 
AGCACGTTCC GTATCCGCTT GCGTTGGACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032575.2
CDS	88..3540
Translation	MKLSRQFTVFGSAIFCVVIFSLYLMLDRGHLDYPRGPRQEGSFPQGQLSILQEKIDHLERLLAENNEIISNIRDSVINLSESVEDGPRGSPGNASQGSIHLHSPQLALQADPRDCLFASQSGSQPRDVQMLDVYDLIPFDNPDGGVWKQGFDIKYEADEWDHEPLQVFVVPHSHNDPGWLKTFNDYFRDKTQYIFNNMVLKLKEDSSRKFMWSEISYLAKWWDIIDIPKKEAVKSLLQNGQLEIVTGGWVMPDEATPHYFALIDQLIEGHQWLEKNLGVKPRSGWAIDPFGHSPTMAYLLKRAGFSHMLIQRVHYAIKKHFSLHKTLEFFWRQNWDLGSATDILCHMMPFYSYDIPHTCGPDPKICCQFDFKRLPGGRYGCPWGVPPEAISPGNVQSRAQMLLDQYRKKSKLFRTKVLLAPLGDDFRFSEYTEWDLQCRNYEQLFSYMNSQPHLKVKIQFGTLSDYFDALEKAVAAEKKSSQSVFPALSGDFFTYADRDDHYWSGYFTSRPFYKRMDRIMESRIRAAEILYQLALKQAQKYKINKFLSSPHYTTLTEARRNLGLFQHHDAITGTAKDWVVVDYGTRLFQSLNSLEKIIGDSAFLLILKDKKLYQSDPSKAFLEMDTKQSSQDSLPQKIIIQLSAQEPRYLVVYNPFEQERHSVVSIRVNSATVKVLSDSGKPVEVQVSAVWNDMRTISQAAYEVSFLAHIPPLGLKVFKILESQSSSSHLADYVLYNNDGLAENGIFHVKNMVDAGDAITIENPFLAIWFDRSGLMEKVRRKEDSRQHELKVQFLWYGTTNKRDKSGAYLFLPDGQGQPYVSLRPPFVRVTRGRIYSDVTCFLEHVTHKVRLYNIQGIEGQSMEVSNIVNIRNVHNREIVMRISSKINNQNRYYTDLNGYQIQPRRTMSKLPLQANVYPMCTMAYIQDAEHRLTLLSAQSLGASSMASGQIEVFMDRRLMQDDNRGLGQGVHDNKITANLFRILLEKRSAVNMEEEKKSPVSYPSLLSHMTSSFLNHPFLPMVLSGQLPSPAFELLSEFPLLQSSLPCDIHLVNLRTIQSKMGKGYSDEAALILHRKGFDCQFSSRGIGLPCSTTQGKMSVLKLFNKFAVESLVPSSLSLMHSPPDAQNMSEVSLSPMEISTFRIRLRWT

Target ORF information:

RefSeq Version	NM_008549.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mannosidase 2, alpha 1 (Man2a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008549.2

ORF Insert Sequence:

ATGAAGTTAA GTCGCCAGTT CACCGTGTTT GGCAGCGCGA TCTTCTGCGT CGTAATCTTC 
TCACTCTACC TGATGCTGGA CAGGGGTCAC TTGGACTACC CTCGGGGCCC GCGCCAGGAG 
GGCTCCTTTC CGCAGGGCCA GCTTTCAATA TTGCAAGAAA AGATTGACCA TTTGGAGCGT 
TTGCTCGCTG AGAACAACGA GATCATCTCA AATATCAGAG ACTCAGTCAT CAACCTGAGC 
GAGTCTGTGG AGGACGGCCC GCGGGGGTCA CCAGGCAACG CCAGCCAAGG CTCCATCCAC 
CTCCACTCGC CACAGTTGGC CCTGCAGGCT GACCCCAGAG ACTGTTTGTT TGCTTCACAG 
AGTGGGAGTC AGCCCCGGGA TGTGCAGATG TTGGATGTTT ACGATCTGAT TCCTTTTGAT 
AATCCAGATG GTGGAGTTTG GAAGCAAGGA TTTGACATTA AGTATGAAGC GGATGAGTGG 
GACCATGAGC CCCTGCAAGT GTTTGTGGTG CCTCACTCCC ATAATGACCC AGGTTGGTTG 
AAGACTTTCA ATGACTACTT TAGAGACAAG ACTCAGTATA TTTTTAATAA CATGGTCCTA 
AAGCTGAAAG AAGACTCAAG CAGGAAGTTT ATGTGGTCTG AGATCTCTTA CCTTGCAAAA 
TGGTGGGATA TTATAGATAT TCCGAAGAAG GAAGCTGTTA AAAGTTTACT ACAGAATGGT 
CAGCTGGAAA TTGTGACCGG TGGCTGGGTT ATGCCTGATG AAGCCACTCC ACATTATTTT 
GCCTTAATTG ACCAACTAAT TGAAGGGCAC CAATGGCTGG AAAAAAATCT AGGAGTGAAA 
CCTCGATCGG GCTGGGCCAT AGATCCCTTT GGTCATTCAC CCACAATGGC TTATCTTCTA 
AAGCGTGCTG GATTTTCACA CATGCTCATC CAGAGAGTCC ATTATGCAAT CAAAAAACAC 
TTCTCTTTGC ATAAAACGCT GGAGTTTTTC TGGAGACAGA ATTGGGATCT TGGATCTGCT 
ACAGACATTT TGTGCCATAT GATGCCCTTC TACAGCTACG ACATCCCTCA CACCTGTGGG 
CCTGATCCTA AAATATGCTG CCAGTTTGAT TTTAAACGGC TTCCTGGAGG CAGATATGGT 
TGTCCCTGGG GAGTTCCCCC AGAAGCAATA TCTCCTGGAA ATGTCCAAAG CAGGGCTCAG 
ATGCTATTGG ATCAGTACCG GAAAAAGTCA AAACTTTTCC GCACTAAAGT TCTGCTGGCT 
CCACTGGGAG ACGACTTTCG GTTCAGTGAA TACACAGAGT GGGATCTGCA GTGCAGGAAC 
TACGAGCAAC TGTTCAGTTA CATGAACTCG CAGCCTCATC TGAAAGTGAA GATCCAGTTT 
GGAACCTTGT CAGATTATTT CGACGCATTG GAGAAAGCGG TGGCAGCCGA GAAGAAGAGT 
AGCCAGTCTG TGTTCCCTGC CCTGAGTGGA GACTTCTTCA CGTACGCTGA CAGAGACGAC 
CATTACTGGA GTGGCTACTT CACGTCCAGA CCTTTCTACA AACGAATGGA CAGAATAATG 
GAATCTCGTA TAAGGGCTGC TGAAATTCTT TACCAGTTGG CCTTGAAACA AGCTCAGAAA 
TACAAGATAA ATAAATTTCT TTCATCACCT CATTACACAA CACTGACAGA AGCCAGAAGG 
AACTTAGGAC TATTTCAGCA TCATGATGCC ATCACAGGAA CCGCGAAAGA CTGGGTGGTT 
GTGGACTATG GTACCAGACT CTTTCAGTCA TTAAATTCTT TGGAGAAGAT AATTGGAGAT 
TCTGCATTTC TTCTCATTTT AAAGGACAAA AAGCTGTACC AGTCAGATCC TTCCAAAGCC 
TTCTTAGAGA TGGATACGAA GCAAAGTTCA CAAGATTCTC TGCCCCAAAA AATTATAATA 
CAACTGAGCG CACAGGAGCC AAGGTACCTT GTGGTCTACA ATCCCTTTGA ACAAGAACGG 
CATTCAGTGG TGTCCATCCG GGTAAACTCC GCCACAGTGA AAGTGCTGTC TGATTCGGGA 
AAACCGGTGG AGGTTCAAGT CAGTGCAGTT TGGAACGACA TGAGGACAAT TTCACAAGCA 
GCCTATGAGG TTTCTTTTCT AGCTCATATA CCACCACTGG GACTGAAAGT GTTTAAGATC 
TTAGAGTCAC AAAGTTCAAG CTCACACTTG GCTGATTATG TCCTATATAA TAATGATGGA 
CTAGCAGAAA ATGGAATATT CCACGTGAAG AACATGGTGG ATGCTGGAGA TGCCATAACA 
ATAGAGAATC CCTTCCTGGC GATTTGGTTT GACCGATCTG GGCTGATGGA GAAAGTGAGA 
AGGAAAGAAG ACAGTAGACA GCATGAACTG AAGGTCCAGT TCCTGTGGTA CGGAACCACC 
AACAAAAGGG ACAAGAGCGG TGCCTACCTC TTCCTGCCTG ACGGGCAGGG CCAGCCATAT 
GTTTCCCTAA GACCGCCCTT TGTCAGAGTG ACACGTGGAA GGATCTACTC AGATGTGACC 
TGTTTCCTCG AACACGTTAC TCACAAAGTC CGCCTGTACA ACATTCAGGG AATAGAAGGT 
CAGTCCATGG AAGTTTCTAA TATTGTAAAC ATCAGGAATG TGCATAACCG TGAGATTGTA 
ATGAGAATTT CATCTAAAAT AAACAACCAA AATAGATATT ATACTGACCT AAATGGATAT 
CAGATTCAGC CTAGAAGGAC CATGAGCAAA TTGCCTCTTC AAGCCAACGT TTACCCGATG 
TGCACAATGG CGTATATCCA GGATGCTGAG CACCGGCTCA CGCTGCTCTC TGCTCAGTCT 
CTAGGTGCTT CCAGCATGGC TTCTGGTCAG ATTGAAGTCT TCATGGATCG AAGGCTCATG 
CAGGATGATA ACCGTGGCCT TGGGCAAGGC GTCCATGACA ATAAGATTAC AGCTAATTTG 
TTTCGAATCC TCCTCGAGAA GAGAAGCGCT GTGAACATGG AAGAAGAAAA GAAGAGCCCT 
GTCAGCTACC CTTCCCTCCT CAGCCACATG ACTTCGTCCT TCCTCAACCA TCCCTTTCTC 
CCCATGGTAC TAAGTGGCCA GCTCCCCTCC CCTGCCTTTG AGCTGCTGAG TGAATTTCCT 
CTGCTGCAGT CCTCTCTACC TTGTGATATC CATCTGGTCA ACCTGCGGAC AATACAATCA 
AAGATGGGCA AAGGCTATTC GGATGAGGCA GCCTTGATCC TCCACAGGAA AGGGTTTGAT 
TGCCAGTTCT CCAGCAGAGG CATCGGGCTA CCCTGTTCCA CTACTCAGGG AAAGATGTCA 
GTTCTGAAAC TTTTCAACAA GTTTGCTGTG GAGAGTCTCG TCCCTTCCTC TCTGTCCTTG 
ATGCACTCCC CTCCAGATGC CCAGAACATG AGTGAAGTCA GCCTGAGCCC CATGGAGATC 
AGCACGTTCC GTATCCGCTT GCGTTGGACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Testosterone deficiency accompanied by testicular and epididymal abnormalities in TMF(-/-) mice.
Molecular and cellular endocrinology365(1)52-63(2013 Jan)
Elkis Y,Bel S,Lerer-Goldstein T,Nyska A,Creasy DM,Shpungin S,Nir U

Pilt is a coiled-coil domain-containing protein that localizes at the trans-Golgi complex and regulates its structure.
FEBS letters586(19)3064-70(2012 Sep)
Tamaki H,Sanda M,Katsumata O,Hara Y,Fukaya M,Sakagami H

Mannosidase 2, alpha 1 deficiency is associated with ricin resistance in embryonic stem (ES) cells.
PloS one6(8)e22993(2011)
Wang W,Hale C,Goulding D,Haslam SM,Tissot B,Lindsay C,Michell S,Titball R,Yu J,Toribio AL,Rossi R,Dell A,Bradley A,Dougan G

Neurog2 controls the leading edge of neurogenesis in the mammalian retina.
Developmental biology340(2)490-503(2010 Apr)
Hufnagel RB,Le TT,Riesenberg AL,Brown NL

Mice lacking mannose 6-phosphate uncovering enzyme activity have a milder phenotype than mice deficient for N-acetylglucosamine-1-phosphotransferase activity.
Molecular biology of the cell20(20)4381-9(2009 Oct)
Boonen M,Vogel P,Platt KA,Dahms N,Kornfeld S

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Man2a1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム