Mlph cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mlph
Entrez Gene ID	171531
Full Name	melanophilin
Synonyms	2210418F23Rik,5031433I09Rik,AW228792,D1Wsu84e,Slac-2a,l(1)-3Rk,l1Rk3,ln
General protein information	Preferred Names melanophilin Names melanophilin exophilin-3 leaden protein slaC2-a slp homolog lacking C2 domains a synaptotagmin-like protein 2a
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 D\|1 45.73 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006529231.4	XP_006529294.1	melanophilin isoform X1
NM_053015.3	NP_443748.2	melanophilin
XM_006529231.3	XP_006529294.1	melanophilin isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MLPH	NP_001096689.2
Danio rerio (zebrafish)	LOC557015	XP_005167685.1
Homo sapiens (human)	MLPH	NP_077006.1
Pan troglodytes (chimpanzee)	MLPH	XP_516180.3
Mus musculus (house mouse)	Mlph	NP_443748.2
Rattus norvegicus (Norway rat)	Mlph	NP_001012135.1
Bos taurus (cattle)	MLPH	NP_001075066.1

Gene Ontology
Bibliography

Related articles in PubMed

Myosin Va's adaptor protein melanophilin enforces track selection on the microtubule and actin networks in vitro.
Oberhofer A, Spieler P, Rosenfeld Y, Stepp WL, Cleetus A, Hume AN, Mueller-Planitz F, ?kten Z
Proceedings of the National Academy of Sciences of the United States of America114(24)E4714-E4723(2017 Jun)

Sequential and compartmentalized action of Rabs, SNAREs, and MAL in the apical delivery of fusiform vesicles in urothelial umbrella cells.
Wankel B, Ouyang J, Guo X, Hadjiolova K, Miller J, Liao Y, Tham DK, Romih R, Andrade LR, Gumper I, Simon JP, Sachdeva R, Tolmachova T, Seabra MC, Fukuda M, Schaeren-Wiemers N, Hong WJ, Sabatini DD, Wu XR, Kong X, Kreibich G, Rindler MJ, Sun TT
Molecular biology of the cell27(10)1621-34(2016 05)

Thyroid hormone is required for the pruning of afferent type II spiral ganglion neurons in the mouse cochlea.
Sundaresan S, Balasubbu S, Mustapha M
Neuroscience312165-78(2016 Jan)

The GTPase-deficient Rab27A(Q78L) mutant inhibits melanosome transport in melanocytes through trapping of Rab27A effector protein Slac2-a/melanophilin in their cytosol: development of a novel melanosome-targetinG tag.
Ishida M, Arai SP, Ohbayashi N, Fukuda M
The Journal of biological chemistry289(16)11059-67(2014 Apr)

More than just a cargo adapter, melanophilin prolongs and slows processive runs of myosin Va.
Sckolnick M, Krementsova EB, Warshaw DM, Trybus KM
The Journal of biological chemistry288(41)29313-22(2013 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results showed that Rab27A(Q78L) is unable to localize on mature melanosomes and that its inhibitory activity on melanosome transport is completely dependent on its binding to the Rab27A effector Slac2-a/melanophilin.
Title: The GTPase-deficient Rab27A(Q78L) mutant inhibits melanosome transport in melanocytes through trapping of Rab27A effector protein Slac2-a/melanophilin in their cytosol: development of a novel melanosome-targetinG tag.

Data show at the single-molecule level that melanophilin (Mlph) increases the number of processively moving myoVa motors by 17-fold.
Title: More than just a cargo adapter, melanophilin prolongs and slows processive runs of myosin Va.

Data suggest that Rab27a and Rab27b regulate distinct steps in the bone marrow-derived mast cells (BMMC) degranulation pathway, with Rab27a/Mlph/MyoVa regulating cortical actin stability upstream of Rab27a/b/Munc13-4-dependent granule exocytosis.
Title: Distinct and opposing roles for Rab27a/Mlph/MyoVa and Rab27b/Munc13-4 in mast cell secretion.

structural basis for the exclusive association of Slac2-a with the Rab27 subfamily.
Title: Structural basis for the exclusive specificity of Slac2-a/melanophilin for the Rab27 GTPases.

Data suggest that Mlph is targeted to and/or stabilised on melanosomes by Rab27a, and then recruits MyoVa, which provides additional stability to the complex and allows melanosomes to transfer from MT to actin-based transport.
Title: Rab27a and MyoVa are the primary Mlph interactors regulating melanosome transport in melanocytes.

The following Mlph gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mlph cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu09008	NM_053015.3 Latest version!	Mus musculus melanophilin (Mlph), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$349.30 $499.00
OMu27985	XM_006529231.4 Latest version!	Mus musculus melanophilin (Mlph), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu27985	XM_006529231.3	Mus musculus melanophilin (Mlph), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mlph ( NM_053015.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_443748.2 Mus musculus melanophilin (Mlph), mRNA.

Price & Availability↑

CloneID	OMu09008
Clone ID Related Accession (Same CDS sequence)	NM_053015.3
Accession Version	NM_053015.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	melanophilin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC118682.8, AK019883.1 and AK148274.1. On Jun 19, 2009 this sequence version replaced NM_053015.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF384098.1, AK148274.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849376 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAAAA GGTTGGACCT TTCCACGCTC ACAGACGAGG AGGCTGAGCA CGTGTGGGCA 
GTGGTTCAGC GGGACTTTGA CCTCAGGAGG CGAGAGGAAG AAAGACTCCA GGGGCTGAAG 
GGCAAAATAC AAAAGGAGAG CTCCAAGAGG GAGCTGCTGT CGGACACAGC CCATCTGAAT 
GAGACTCACT GTGCCCGCTG CCTGCAGCCC TACCGGCTGC TCCTGAACAG CAGACGACAG 
TGCCTAGAGT GCAGCCTCTT CGTCTGCAAA AGCTGCAGCC ACGCCCACCC AGAAGAGCAG 
GGCTGGCTCT GCGACCCCTG CCACCTGGCC AGGGTCGTGA AGATCGGTTC TCTGGAGTGG 
TACTACCAGC ACGTGAGGGC TCGCTTCAAG CGTTTCGGGA GTGCCAAAGT GATCCGGTCT 
CTCTGTGGGC GGCTGCAGGG TGGAGGTGGA TCTGAGCCAA GCTTGGAAGA AGGAAATGGA 
GACAGTGAGC AGACTGATGA GGATGGAGAC CTGGACACAG AGGCCAGAGA CCAGCCCCTC 
AACAGCAAAA AGAAAAAGCG CCTGCTCTCC TTCCGAGATG TGGACTTTGA GGAAGACTCA 
GACCACTTGG TGCAGCCTTG CAGCCAAACC TTGGGCCTGT CCTCAGTCCC TGAGTCTGCA 
CACAGCCTGC AGTCCCTCTC AGGTGAGCCC TACTCTGAGG ACACCACCTC TCTGGAGCCC 
GAGGGCCTAG AGGAGACTGG TGCAAGGGCT TTGGGATGTC ATCCCAGTCC TGAAGTGCAG 
CCATGTAGCC CTTTACCCTC TGGGGAGGAT GCTCACGCTG AACTGGACTC GCCTGCAGCA 
TCCTGCAAGA GTGCCTTTGG GACCACAGCT ATGCCTGGAA CAGACGATGT CAGGGGCAAA 
CATCTGCCCT CACAGTACCT GGCTGATGTA GACACCTCTG ATGAAGACAG TATCCAGGGT 
CCCAGGGCAG CCTCCCAGCA TAGCAAGAGG AGGGCCCGGA CTGTGCCTGA GACTCAGATC 
TTGGAGTTGA ACAAGCGAAT GTCAGCTGTG GAGCACCTGC TAGTCCACCT GGAGAATACG 
GTTCTGCCAC CCTCAGCCCA GGAACCAACT GTGGAGACAC ACCCCAGTGC TGACACAGAG 
GAGGAGACAC TCAGGAGGAG GCTGGAGGAG CTGACCAGCA ACATCAGTGG TTCCAGCACC 
TCATCAGAAG ATGAGACCAA GCCAGATGGC ACCTTCCTTG GAGGGTCCCC AAAGGTGTGC 
ACAGACACAG GGCACATGGA GACACAGGAA AGGAACCCTC GGAGCCCTGG GAACCCTGCT 
CGGCCTACAA AAAGCACAGA TGAGGAGCTC TCTGAGATGG AGGATAGAGT GGCCATGACA 
GCCTCTGAAG TTCAGCAGGC TGAGAGTGAG ATCTCAGACA TCCAGTCCAG GATCGCAGCT 
CTGAGAGCCG CAGGACTCAC AGTGAAGCCC TCGGGAAAAC CTCGGAGAAA GTCAGGCATC 
CCGATCTTTC TTCCCCGCGT TACTGAGAAA CTTGACAGGA TCCCAAAGAC TCCACCTGCA 
GACCCTGATG ACCAAGCCAA GATGCCCAAG GCAACAACAG CTGTGCCCTC TCTCCTGAGG 
AGGAAGTATT CTCCCAGCAG CCAAGGCGTA GACAGTGGTT CTTTTGATCG GAAATCAGTG 
TACCGTGGCT CGCTGACACA AAGGAACCCT AACGGGAGGA GAGGGACAGC CAGACACATC 
TTCGCGAAAC CCGTGATGGC CCAGCAGCCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_443748.2
CDS	179..1951
Translation	MGKRLDLSTLTDEEAEHVWAVVQRDFDLRRREEERLQGLKGKIQKESSKRELLSDTAHLNETHCARCLQPYRLLLNSRRQCLECSLFVCKSCSHAHPEEQGWLCDPCHLARVVKIGSLEWYYQHVRARFKRFGSAKVIRSLCGRLQGGGGSEPSLEEGNGDSEQTDEDGDLDTEARDQPLNSKKKKRLLSFRDVDFEEDSDHLVQPCSQTLGLSSVPESAHSLQSLSGEPYSEDTTSLEPEGLEETGARALGCHPSPEVQPCSPLPSGEDAHAELDSPAASCKSAFGTTAMPGTDDVRGKHLPSQYLADVDTSDEDSIQGPRAASQHSKRRARTVPETQILELNKRMSAVEHLLVHLENTVLPPSAQEPTVETHPSADTEEETLRRRLEELTSNISGSSTSSEDETKPDGTFLGGSPKVCTDTGHMETQERNPRSPGNPARPTKSTDEELSEMEDRVAMTASEVQQAESEISDIQSRIAALRAAGLTVKPSGKPRRKSGIPIFLPRVTEKLDRIPKTPPADPDDQAKMPKATTAVPSLLRRKYSPSSQGVDSGSFDRKSVYRGSLTQRNPNGRRGTARHIFAKPVMAQQP

Target ORF information:

RefSeq Version	NM_053015.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus melanophilin (Mlph), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053015.3

ORF Insert Sequence:

ATGGGGAAAA GGTTGGACCT TTCCACGCTC ACAGACGAGG AGGCTGAGCA CGTGTGGGCA 
GTGGTTCAGC GGGACTTTGA CCTCAGGAGG CGAGAGGAAG AAAGACTCCA GGGGCTGAAG 
GGCAAAATAC AAAAGGAGAG CTCCAAGAGG GAGCTGCTGT CGGACACAGC CCATCTGAAT 
GAGACTCACT GTGCCCGCTG CCTGCAGCCC TACCGGCTGC TCCTGAACAG CAGACGACAG 
TGCCTAGAGT GCAGCCTCTT CGTCTGCAAA AGCTGCAGCC ACGCCCACCC AGAAGAGCAG 
GGCTGGCTCT GCGACCCCTG CCACCTGGCC AGGGTCGTGA AGATCGGTTC TCTGGAGTGG 
TACTACCAGC ACGTGAGGGC TCGCTTCAAG CGTTTCGGGA GTGCCAAAGT GATCCGGTCT 
CTCTGTGGGC GGCTGCAGGG TGGAGGTGGA TCTGAGCCAA GCTTGGAAGA AGGAAATGGA 
GACAGTGAGC AGACTGATGA GGATGGAGAC CTGGACACAG AGGCCAGAGA CCAGCCCCTC 
AACAGCAAAA AGAAAAAGCG CCTGCTCTCC TTCCGAGATG TGGACTTTGA GGAAGACTCA 
GACCACTTGG TGCAGCCTTG CAGCCAAACC TTGGGCCTGT CCTCAGTCCC TGAGTCTGCA 
CACAGCCTGC AGTCCCTCTC AGGTGAGCCC TACTCTGAGG ACACCACCTC TCTGGAGCCC 
GAGGGCCTAG AGGAGACTGG TGCAAGGGCT TTGGGATGTC ATCCCAGTCC TGAAGTGCAG 
CCATGTAGCC CTTTACCCTC TGGGGAGGAT GCTCACGCTG AACTGGACTC GCCTGCAGCA 
TCCTGCAAGA GTGCCTTTGG GACCACAGCT ATGCCTGGAA CAGACGATGT CAGGGGCAAA 
CATCTGCCCT CACAGTACCT GGCTGATGTA GACACCTCTG ATGAAGACAG TATCCAGGGT 
CCCAGGGCAG CCTCCCAGCA TAGCAAGAGG AGGGCCCGGA CTGTGCCTGA GACTCAGATC 
TTGGAGTTGA ACAAGCGAAT GTCAGCTGTG GAGCACCTGC TAGTCCACCT GGAGAATACG 
GTTCTGCCAC CCTCAGCCCA GGAACCAACT GTGGAGACAC ACCCCAGTGC TGACACAGAG 
GAGGAGACAC TCAGGAGGAG GCTGGAGGAG CTGACCAGCA ACATCAGTGG TTCCAGCACC 
TCATCAGAAG ATGAGACCAA GCCAGATGGC ACCTTCCTTG GAGGGTCCCC AAAGGTGTGC 
ACAGACACAG GGCACATGGA GACACAGGAA AGGAACCCTC GGAGCCCTGG GAACCCTGCT 
CGGCCTACAA AAAGCACAGA TGAGGAGCTC TCTGAGATGG AGGATAGAGT GGCCATGACA 
GCCTCTGAAG TTCAGCAGGC TGAGAGTGAG ATCTCAGACA TCCAGTCCAG GATCGCAGCT 
CTGAGAGCCG CAGGACTCAC AGTGAAGCCC TCGGGAAAAC CTCGGAGAAA GTCAGGCATC 
CCGATCTTTC TTCCCCGCGT TACTGAGAAA CTTGACAGGA TCCCAAAGAC TCCACCTGCA 
GACCCTGATG ACCAAGCCAA GATGCCCAAG GCAACAACAG CTGTGCCCTC TCTCCTGAGG 
AGGAAGTATT CTCCCAGCAG CCAAGGCGTA GACAGTGGTT CTTTTGATCG GAAATCAGTG 
TACCGTGGCT CGCTGACACA AAGGAACCCT AACGGGAGGA GAGGGACAGC CAGACACATC 
TTCGCGAAAC CCGTGATGGC CCAGCAGCCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mlph ( XM_006529231.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529294.1 Mus musculus melanophilin (Mlph), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu27985
Clone ID Related Accession (Same CDS sequence)	XM_006529231.4 , XM_006529231.3
Accession Version	XM_006529231.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	melanophilin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529231.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAAA GGTTGGACCT TTCCACGCTC ACAGACGAGG AGGCTGAGCA CGTGTGGGCA 
GTGGTTCAGC GGGACTTTGA CCTCAGGAGG CGAGAGGAAG AAAGACTCCA GGGGCTGAAG 
GGCAAAATAC AAAAGGAGAG CTCCAAGAGG GAGCTGCTGT CGGACACAGC CCATCTGAAT 
GAGACTCACT GTGCCCGCTG CCTGCAGCCC TACCGGCTGC TCCTGAACAG CAGACGACAG 
TGCCTAGAGT GCAGCCTCTT CGTCTGCAAA AGCTGCAGCC ACGCCCACCC AGAAGAGCAG 
GGCTGGCTCT GCGACCCCTG CCACCTGGCC AGGGTCGTGA AGATCGGTTC TCTGGAGTGG 
TACTACCAGC ACGTGAGGGC TCGCTTCAAG CGTTTCGGGA GTGCCAAAGT GATCCGGTCT 
CTCTGTGGGC GGCTGCAGGG TGGAGGTGGA TCTGAGCCAA GCTTGGAAGA AGGAAATGGA 
GACAGTGAGC AGACTGATGA GGATGGAGAC CTGGACACAG AGGCCAGAGA CCAGCCCCTC 
AACAGCAAAA AAAAGCGCCT GCTCTCCTTC CGAGATGTGG ACTTTGAGGA AGACTCAGAC 
CACTTGGTGC AGCCTTGCAG CCAAACCTTG GGCCTGTCCT CAGTCCCTGA GTCTGCACAC 
AGCCTGCAGT CCCTCTCAGG TGAGCCCTAC TCTGAGGACA CCACCTCTCT GGAGCCCGAG 
GGCCTAGAGG AGACTGGTGC AAGGGCTTTG GGATGTCATC CCAGTCCTGA AGTGCAGCCA 
TGTAGCCCTT TACCCTCTGG GGAGGATGCT CACGCTGAAC TGGACTCGCC TGCAGCATCC 
TGCAAGAGTG CCTTTGGGAC CACAGCTATG CCTGGAACAG ACGATGTCAG GGGCAAACAT 
CTGCCCTCAC AGTACCTGGC TGATGTAGAC ACCTCTGATG AAGACAGTAT CCAGGGTCCC 
AGGGCAGCCT CCCAGCATAG CAAGAGGAGG GCCCGGACTG TGCCTGAGAC TCAGATCTTG 
GAGTTGAACA AGCGAATGTC AGCTGTGGAG CACCTGCTAG TCCACCTGGA GAATACGGTT 
CTGCCACCCT CAGCCCAGGA ACCAACTGTG GAGACACACC CCAGTGCTGA CACAGAGGAG 
GAGACACTCA GGAGGAGGCT GGAGGAGCTG ACCAGCAACA TCAGTGGTTC CAGCACCTCA 
TCAGAAGATG AGACCAAGCC AGATGGCACC TTCCTTGGAG GGTCCCCAAA GGTGTGCACA 
GACACAGGGC ACATGGAGAC ACAGGAAAGG AACCCTCGGA GCCCTGGGAA CCCTGCTCGG 
CCTACAAAAA GCACAGATGA GGAGCTCTCT GAGATGGAGG ATAGAGTGGC CATGACAGCC 
TCTGAAGTTC AGCAGGCTGA GAGTGAGATC TCAGACATCC AGTCCAGGAT CGCAGCTCTG 
AGAGCCGCAG GACTCACAGT GAAGCCCTCG GGAAAACCTC GGAGAAAGTC AGGCATCCCG 
ATCTTTCTTC CCCGCGTTAC TGAGAAACTT GACAGGATCC CAAAGACTCC ACCTGCAGAC 
CCTGATGACC AAGCCAAGAT GCCCAAGGCA ACAACAGCTG TGCCCTCTCT CCTGAGGAGG 
AAGTATTCTC CCAGCAGCCA AGGCGTAGAC AGTGGTTCTT TTGATCGGAA ATCAGTGTAC 
CGTGGCTCGC TGACACAAAG GAACCCTAAC GGGAGGAGAG GGACAGCCAG ACACATCTTC 
GCGAAACCCG TGATGGCCCA GCAGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529294.1
CDS	256..2025
Translation	MGKRLDLSTLTDEEAEHVWAVVQRDFDLRRREEERLQGLKGKIQKESSKRELLSDTAHLNETHCARCLQPYRLLLNSRRQCLECSLFVCKSCSHAHPEEQGWLCDPCHLARVVKIGSLEWYYQHVRARFKRFGSAKVIRSLCGRLQGGGGSEPSLEEGNGDSEQTDEDGDLDTEARDQPLNSKKKRLLSFRDVDFEEDSDHLVQPCSQTLGLSSVPESAHSLQSLSGEPYSEDTTSLEPEGLEETGARALGCHPSPEVQPCSPLPSGEDAHAELDSPAASCKSAFGTTAMPGTDDVRGKHLPSQYLADVDTSDEDSIQGPRAASQHSKRRARTVPETQILELNKRMSAVEHLLVHLENTVLPPSAQEPTVETHPSADTEEETLRRRLEELTSNISGSSTSSEDETKPDGTFLGGSPKVCTDTGHMETQERNPRSPGNPARPTKSTDEELSEMEDRVAMTASEVQQAESEISDIQSRIAALRAAGLTVKPSGKPRRKSGIPIFLPRVTEKLDRIPKTPPADPDDQAKMPKATTAVPSLLRRKYSPSSQGVDSGSFDRKSVYRGSLTQRNPNGRRGTARHIFAKPVMAQQP

Target ORF information:

RefSeq Version	XM_006529231.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus melanophilin (Mlph), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529231.3

ORF Insert Sequence:

ATGGGGAAAA GGTTGGACCT TTCCACGCTC ACAGACGAGG AGGCTGAGCA CGTGTGGGCA 
GTGGTTCAGC GGGACTTTGA CCTCAGGAGG CGAGAGGAAG AAAGACTCCA GGGGCTGAAG 
GGCAAAATAC AAAAGGAGAG CTCCAAGAGG GAGCTGCTGT CGGACACAGC CCATCTGAAT 
GAGACTCACT GTGCCCGCTG CCTGCAGCCC TACCGGCTGC TCCTGAACAG CAGACGACAG 
TGCCTAGAGT GCAGCCTCTT CGTCTGCAAA AGCTGCAGCC ACGCCCACCC AGAAGAGCAG 
GGCTGGCTCT GCGACCCCTG CCACCTGGCC AGGGTCGTGA AGATCGGTTC TCTGGAGTGG 
TACTACCAGC ACGTGAGGGC TCGCTTCAAG CGTTTCGGGA GTGCCAAAGT GATCCGGTCT 
CTCTGTGGGC GGCTGCAGGG TGGAGGTGGA TCTGAGCCAA GCTTGGAAGA AGGAAATGGA 
GACAGTGAGC AGACTGATGA GGATGGAGAC CTGGACACAG AGGCCAGAGA CCAGCCCCTC 
AACAGCAAAA AAAAGCGCCT GCTCTCCTTC CGAGATGTGG ACTTTGAGGA AGACTCAGAC 
CACTTGGTGC AGCCTTGCAG CCAAACCTTG GGCCTGTCCT CAGTCCCTGA GTCTGCACAC 
AGCCTGCAGT CCCTCTCAGG TGAGCCCTAC TCTGAGGACA CCACCTCTCT GGAGCCCGAG 
GGCCTAGAGG AGACTGGTGC AAGGGCTTTG GGATGTCATC CCAGTCCTGA AGTGCAGCCA 
TGTAGCCCTT TACCCTCTGG GGAGGATGCT CACGCTGAAC TGGACTCGCC TGCAGCATCC 
TGCAAGAGTG CCTTTGGGAC CACAGCTATG CCTGGAACAG ACGATGTCAG GGGCAAACAT 
CTGCCCTCAC AGTACCTGGC TGATGTAGAC ACCTCTGATG AAGACAGTAT CCAGGGTCCC 
AGGGCAGCCT CCCAGCATAG CAAGAGGAGG GCCCGGACTG TGCCTGAGAC TCAGATCTTG 
GAGTTGAACA AGCGAATGTC AGCTGTGGAG CACCTGCTAG TCCACCTGGA GAATACGGTT 
CTGCCACCCT CAGCCCAGGA ACCAACTGTG GAGACACACC CCAGTGCTGA CACAGAGGAG 
GAGACACTCA GGAGGAGGCT GGAGGAGCTG ACCAGCAACA TCAGTGGTTC CAGCACCTCA 
TCAGAAGATG AGACCAAGCC AGATGGCACC TTCCTTGGAG GGTCCCCAAA GGTGTGCACA 
GACACAGGGC ACATGGAGAC ACAGGAAAGG AACCCTCGGA GCCCTGGGAA CCCTGCTCGG 
CCTACAAAAA GCACAGATGA GGAGCTCTCT GAGATGGAGG ATAGAGTGGC CATGACAGCC 
TCTGAAGTTC AGCAGGCTGA GAGTGAGATC TCAGACATCC AGTCCAGGAT CGCAGCTCTG 
AGAGCCGCAG GACTCACAGT GAAGCCCTCG GGAAAACCTC GGAGAAAGTC AGGCATCCCG 
ATCTTTCTTC CCCGCGTTAC TGAGAAACTT GACAGGATCC CAAAGACTCC ACCTGCAGAC 
CCTGATGACC AAGCCAAGAT GCCCAAGGCA ACAACAGCTG TGCCCTCTCT CCTGAGGAGG 
AAGTATTCTC CCAGCAGCCA AGGCGTAGAC AGTGGTTCTT TTGATCGGAA ATCAGTGTAC 
CGTGGCTCGC TGACACAAAG GAACCCTAAC GGGAGGAGAG GGACAGCCAG ACACATCTTC 
GCGAAACCCG TGATGGCCCA GCAGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mlph ( XM_006529231.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529294.1 Mus musculus melanophilin (Mlph), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu27985
Clone ID Related Accession (Same CDS sequence)	XM_006529231.4 , XM_006529231.3
Accession Version	XM_006529231.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	melanophilin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529231.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAAA GGTTGGACCT TTCCACGCTC ACAGACGAGG AGGCTGAGCA CGTGTGGGCA 
GTGGTTCAGC GGGACTTTGA CCTCAGGAGG CGAGAGGAAG AAAGACTCCA GGGGCTGAAG 
GGCAAAATAC AAAAGGAGAG CTCCAAGAGG GAGCTGCTGT CGGACACAGC CCATCTGAAT 
GAGACTCACT GTGCCCGCTG CCTGCAGCCC TACCGGCTGC TCCTGAACAG CAGACGACAG 
TGCCTAGAGT GCAGCCTCTT CGTCTGCAAA AGCTGCAGCC ACGCCCACCC AGAAGAGCAG 
GGCTGGCTCT GCGACCCCTG CCACCTGGCC AGGGTCGTGA AGATCGGTTC TCTGGAGTGG 
TACTACCAGC ACGTGAGGGC TCGCTTCAAG CGTTTCGGGA GTGCCAAAGT GATCCGGTCT 
CTCTGTGGGC GGCTGCAGGG TGGAGGTGGA TCTGAGCCAA GCTTGGAAGA AGGAAATGGA 
GACAGTGAGC AGACTGATGA GGATGGAGAC CTGGACACAG AGGCCAGAGA CCAGCCCCTC 
AACAGCAAAA AAAAGCGCCT GCTCTCCTTC CGAGATGTGG ACTTTGAGGA AGACTCAGAC 
CACTTGGTGC AGCCTTGCAG CCAAACCTTG GGCCTGTCCT CAGTCCCTGA GTCTGCACAC 
AGCCTGCAGT CCCTCTCAGG TGAGCCCTAC TCTGAGGACA CCACCTCTCT GGAGCCCGAG 
GGCCTAGAGG AGACTGGTGC AAGGGCTTTG GGATGTCATC CCAGTCCTGA AGTGCAGCCA 
TGTAGCCCTT TACCCTCTGG GGAGGATGCT CACGCTGAAC TGGACTCGCC TGCAGCATCC 
TGCAAGAGTG CCTTTGGGAC CACAGCTATG CCTGGAACAG ACGATGTCAG GGGCAAACAT 
CTGCCCTCAC AGTACCTGGC TGATGTAGAC ACCTCTGATG AAGACAGTAT CCAGGGTCCC 
AGGGCAGCCT CCCAGCATAG CAAGAGGAGG GCCCGGACTG TGCCTGAGAC TCAGATCTTG 
GAGTTGAACA AGCGAATGTC AGCTGTGGAG CACCTGCTAG TCCACCTGGA GAATACGGTT 
CTGCCACCCT CAGCCCAGGA ACCAACTGTG GAGACACACC CCAGTGCTGA CACAGAGGAG 
GAGACACTCA GGAGGAGGCT GGAGGAGCTG ACCAGCAACA TCAGTGGTTC CAGCACCTCA 
TCAGAAGATG AGACCAAGCC AGATGGCACC TTCCTTGGAG GGTCCCCAAA GGTGTGCACA 
GACACAGGGC ACATGGAGAC ACAGGAAAGG AACCCTCGGA GCCCTGGGAA CCCTGCTCGG 
CCTACAAAAA GCACAGATGA GGAGCTCTCT GAGATGGAGG ATAGAGTGGC CATGACAGCC 
TCTGAAGTTC AGCAGGCTGA GAGTGAGATC TCAGACATCC AGTCCAGGAT CGCAGCTCTG 
AGAGCCGCAG GACTCACAGT GAAGCCCTCG GGAAAACCTC GGAGAAAGTC AGGCATCCCG 
ATCTTTCTTC CCCGCGTTAC TGAGAAACTT GACAGGATCC CAAAGACTCC ACCTGCAGAC 
CCTGATGACC AAGCCAAGAT GCCCAAGGCA ACAACAGCTG TGCCCTCTCT CCTGAGGAGG 
AAGTATTCTC CCAGCAGCCA AGGCGTAGAC AGTGGTTCTT TTGATCGGAA ATCAGTGTAC 
CGTGGCTCGC TGACACAAAG GAACCCTAAC GGGAGGAGAG GGACAGCCAG ACACATCTTC 
GCGAAACCCG TGATGGCCCA GCAGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529294.1
CDS	255..2024
Translation	MGKRLDLSTLTDEEAEHVWAVVQRDFDLRRREEERLQGLKGKIQKESSKRELLSDTAHLNETHCARCLQPYRLLLNSRRQCLECSLFVCKSCSHAHPEEQGWLCDPCHLARVVKIGSLEWYYQHVRARFKRFGSAKVIRSLCGRLQGGGGSEPSLEEGNGDSEQTDEDGDLDTEARDQPLNSKKKRLLSFRDVDFEEDSDHLVQPCSQTLGLSSVPESAHSLQSLSGEPYSEDTTSLEPEGLEETGARALGCHPSPEVQPCSPLPSGEDAHAELDSPAASCKSAFGTTAMPGTDDVRGKHLPSQYLADVDTSDEDSIQGPRAASQHSKRRARTVPETQILELNKRMSAVEHLLVHLENTVLPPSAQEPTVETHPSADTEEETLRRRLEELTSNISGSSTSSEDETKPDGTFLGGSPKVCTDTGHMETQERNPRSPGNPARPTKSTDEELSEMEDRVAMTASEVQQAESEISDIQSRIAALRAAGLTVKPSGKPRRKSGIPIFLPRVTEKLDRIPKTPPADPDDQAKMPKATTAVPSLLRRKYSPSSQGVDSGSFDRKSVYRGSLTQRNPNGRRGTARHIFAKPVMAQQP

Target ORF information:

RefSeq Version	XM_006529231.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus melanophilin (Mlph), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529231.4

ORF Insert Sequence:

ATGGGGAAAA GGTTGGACCT TTCCACGCTC ACAGACGAGG AGGCTGAGCA CGTGTGGGCA 
GTGGTTCAGC GGGACTTTGA CCTCAGGAGG CGAGAGGAAG AAAGACTCCA GGGGCTGAAG 
GGCAAAATAC AAAAGGAGAG CTCCAAGAGG GAGCTGCTGT CGGACACAGC CCATCTGAAT 
GAGACTCACT GTGCCCGCTG CCTGCAGCCC TACCGGCTGC TCCTGAACAG CAGACGACAG 
TGCCTAGAGT GCAGCCTCTT CGTCTGCAAA AGCTGCAGCC ACGCCCACCC AGAAGAGCAG 
GGCTGGCTCT GCGACCCCTG CCACCTGGCC AGGGTCGTGA AGATCGGTTC TCTGGAGTGG 
TACTACCAGC ACGTGAGGGC TCGCTTCAAG CGTTTCGGGA GTGCCAAAGT GATCCGGTCT 
CTCTGTGGGC GGCTGCAGGG TGGAGGTGGA TCTGAGCCAA GCTTGGAAGA AGGAAATGGA 
GACAGTGAGC AGACTGATGA GGATGGAGAC CTGGACACAG AGGCCAGAGA CCAGCCCCTC 
AACAGCAAAA AAAAGCGCCT GCTCTCCTTC CGAGATGTGG ACTTTGAGGA AGACTCAGAC 
CACTTGGTGC AGCCTTGCAG CCAAACCTTG GGCCTGTCCT CAGTCCCTGA GTCTGCACAC 
AGCCTGCAGT CCCTCTCAGG TGAGCCCTAC TCTGAGGACA CCACCTCTCT GGAGCCCGAG 
GGCCTAGAGG AGACTGGTGC AAGGGCTTTG GGATGTCATC CCAGTCCTGA AGTGCAGCCA 
TGTAGCCCTT TACCCTCTGG GGAGGATGCT CACGCTGAAC TGGACTCGCC TGCAGCATCC 
TGCAAGAGTG CCTTTGGGAC CACAGCTATG CCTGGAACAG ACGATGTCAG GGGCAAACAT 
CTGCCCTCAC AGTACCTGGC TGATGTAGAC ACCTCTGATG AAGACAGTAT CCAGGGTCCC 
AGGGCAGCCT CCCAGCATAG CAAGAGGAGG GCCCGGACTG TGCCTGAGAC TCAGATCTTG 
GAGTTGAACA AGCGAATGTC AGCTGTGGAG CACCTGCTAG TCCACCTGGA GAATACGGTT 
CTGCCACCCT CAGCCCAGGA ACCAACTGTG GAGACACACC CCAGTGCTGA CACAGAGGAG 
GAGACACTCA GGAGGAGGCT GGAGGAGCTG ACCAGCAACA TCAGTGGTTC CAGCACCTCA 
TCAGAAGATG AGACCAAGCC AGATGGCACC TTCCTTGGAG GGTCCCCAAA GGTGTGCACA 
GACACAGGGC ACATGGAGAC ACAGGAAAGG AACCCTCGGA GCCCTGGGAA CCCTGCTCGG 
CCTACAAAAA GCACAGATGA GGAGCTCTCT GAGATGGAGG ATAGAGTGGC CATGACAGCC 
TCTGAAGTTC AGCAGGCTGA GAGTGAGATC TCAGACATCC AGTCCAGGAT CGCAGCTCTG 
AGAGCCGCAG GACTCACAGT GAAGCCCTCG GGAAAACCTC GGAGAAAGTC AGGCATCCCG 
ATCTTTCTTC CCCGCGTTAC TGAGAAACTT GACAGGATCC CAAAGACTCC ACCTGCAGAC 
CCTGATGACC AAGCCAAGAT GCCCAAGGCA ACAACAGCTG TGCCCTCTCT CCTGAGGAGG 
AAGTATTCTC CCAGCAGCCA AGGCGTAGAC AGTGGTTCTT TTGATCGGAA ATCAGTGTAC 
CGTGGCTCGC TGACACAAAG GAACCCTAAC GGGAGGAGAG GGACAGCCAG ACACATCTTC 
GCGAAACCCG TGATGGCCCA GCAGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Myosin Va's adaptor protein melanophilin enforces track selection on the microtubule and actin networks in vitro.
Proceedings of the National Academy of Sciences of the United States of America114(24)E4714-E4723(2017 Jun)
Oberhofer A,Spieler P,Rosenfeld Y,Stepp WL,Cleetus A,Hume AN,Mueller-Planitz F,?kten Z

Sequential and compartmentalized action of Rabs, SNAREs, and MAL in the apical delivery of fusiform vesicles in urothelial umbrella cells.
Molecular biology of the cell27(10)1621-34(2016 05)
Wankel B,Ouyang J,Guo X,Hadjiolova K,Miller J,Liao Y,Tham DK,Romih R,Andrade LR,Gumper I,Simon JP,Sachdeva R,Tolmachova T,Seabra MC,Fukuda M,Schaeren-Wiemers N,Hong WJ,Sabatini DD,Wu XR,Kong X,Kreibich G,Rindler MJ,Sun TT

Thyroid hormone is required for the pruning of afferent type II spiral ganglion neurons in the mouse cochlea.
Neuroscience312165-78(2016 Jan)
Sundaresan S,Balasubbu S,Mustapha M

The GTPase-deficient Rab27A(Q78L) mutant inhibits melanosome transport in melanocytes through trapping of Rab27A effector protein Slac2-a/melanophilin in their cytosol: development of a novel melanosome-targetinG tag.
The Journal of biological chemistry289(16)11059-67(2014 Apr)
Ishida M,Arai SP,Ohbayashi N,Fukuda M

More than just a cargo adapter, melanophilin prolongs and slows processive runs of myosin Va.
The Journal of biological chemistry288(41)29313-22(2013 Oct)
Sckolnick M,Krementsova EB,Warshaw DM,Trybus KM