Sspn cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sspn
Entrez Gene ID	16651
Full Name	sarcospan
Synonyms	Krag
General protein information	Preferred Names sarcospan Names sarcospan K-ras oncogene-associated protein Kras oncogene-associated kirsten-Ras-associated protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 G3\|6 77.7 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006506916.4	XP_006506979.1	sarcospan isoform X1
NM_001310837.1	NP_001297766.1	sarcospan isoform 2
XM_006506916.3	XP_006506979.1	sarcospan isoform X1
NM_010656.2	NP_034786.1	sarcospan isoform 1
NM_010656.3	NP_034786.1	sarcospan isoform 1

Pathways from BioSystems

Homologies

	SSPN	XP_001102485.1
Rattus norvegicus (Norway rat)	Sspn	NP_001102725.1
Canis lupus familiaris (dog)	SSPN	XP_003433567.2
Gallus gallus (chicken)	SSPN	XP_416438.1
Danio rerio (zebrafish)	sspn	NP_001032669.1
Homo sapiens (human)	SSPN	NP_005077.2
Pan troglodytes (chimpanzee)	SSPN	XP_001144827.1
Mus musculus (house mouse)	Sspn	NP_034786.1
Bos taurus (cattle)	SSPN	NP_001179934.1

Gene Ontology
Bibliography

Related articles in PubMed

Nanospan, an alternatively spliced isoform of sarcospan, localizes to the sarcoplasmic reticulum in skeletal muscle and is absent in limb girdle muscular dystrophy 2F.
Peter AK, Miller G, Capote J, DiFranco M, Solares-P?rez A, Wang EL, Heighway J, Coral-V?zquez RM, Vergara J, Crosbie-Watson RH
Skeletal muscle7(1)11(2017 Jun)

High levels of sarcospan are well tolerated and act as a sarcolemmal stabilizer to address skeletal muscle and pulmonary dysfunction in DMD.
Gibbs EM, Marshall JL, Ma E, Nguyen TM, Hong G, Lam JS, Spencer MJ, Crosbie-Watson RH
Human molecular genetics25(24)5395-5406(2016 12)

Decreased expression levels of Ifi genes is associated to the increased resistance to spontaneous arthritis disease in mice deficiency of IL-1RA.
Liu X, Jiao Y, Cao Y, Deng N, Ma Y, Hasty KA, Kang A, Chen H, Stuart JM, Gu W
BMC immunology17(1)25(2016 08)

Sarcospan Regulates Cardiac Isoproterenol Response and Prevents Duchenne Muscular Dystrophy-Associated Cardiomyopathy.
Parvatiyar MS, Marshall JL, Nguyen RT, Jordan MC, Richardson VA, Roos KP, Crosbie-Watson RH
Journal of the American Heart Association4(12)(2015 Dec)

Sarcospan integration into laminin-binding adhesion complexes that ameliorate muscular dystrophy requires utrophin and ?7 integrin.
Marshall JL, Oh J, Chou E, Lee JA, Holmberg J, Burkin DJ, Crosbie-Watson RH
Human molecular genetics24(7)2011-22(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these results demonstrate that SSPN overexpression is well tolerated in mdx mice and improves sarcolemma defects that underlie skeletal muscle and pulmonary dysfunction in Duchenne muscular dystrophy
Title: High levels of sarcospan are well tolerated and act as a sarcolemmal stabilizer to address skeletal muscle and pulmonary dysfunction in DMD.

regulates cardiac isoproterenol response and prevents Duchenne muscular dystrophy-associated cardiomyopathy
Title: Sarcospan Regulates Cardiac Isoproterenol Response and Prevents Duchenne Muscular Dystrophy-Associated Cardiomyopathy.

Sarcospan-mediated amelioration of muscular dystrophy in mouse model is dependent on the presence of both utrophin and alpha7beta1 integrin.
Title: Sarcospan integration into laminin-binding adhesion complexes that ameliorate muscular dystrophy requires utrophin and ?7 integrin.

SSPN is a necessary component of dystrophin and Utr function and that SSPN modulation of integrin signaling is required for extracellular matrix attachment and muscle force development.
Title: Dystrophin and utrophin expression require sarcospan: loss of ?7 integrin exacerbates a newly discovered muscle phenotype in sarcospan-null mice.

Sarcospan-null mice displayed delayed differentiation after cardiotoxin injury caused by loss of utrophin and Akt signaling
Title: Sarcospan-dependent Akt activation is required for utrophin expression and muscle regeneration.

The following Sspn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sspn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu68103	NM_001310837.1 Latest version!	Mus musculus sarcospan (Sspn), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu06550	NM_010656.3 Latest version!	Mus musculus sarcospan (Sspn), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu37791	XM_006506916.4 Latest version!	Mus musculus sarcospan (Sspn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu37791	XM_006506916.3	Mus musculus sarcospan (Sspn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu06550	NM_010656.2	Mus musculus sarcospan (Sspn), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sspn ( NM_001310837.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001297766.1 Mus musculus sarcospan (Sspn), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu68103
Clone ID Related Accession (Same CDS sequence)	NM_001310837.1
Accession Version	NM_001310837.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	sarcospan isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CA558278.1, CK333705.1, CF915662.1, AK164529.1 and AC144936.4. On Jul 21, 2015 this sequence version replaced XM_006506917.2. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 2, which has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660825.126506.1, CK333705.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTTTGTG TCTCATACCA AGTTGACGAG AGGACATGTG TCCAGTTTTC TATGAAGGTA 
TTCTACTTCC TGTTGAGTGC CCTCGGCCTG ATGGTCTGCA TGCTGGCTGT GGCCTTTGCC 
GCCCACCACT ACTCCCTGCT TGCGCAGTTT ACCTGCGAGA CCTCCCTGGA TTCTTGCCAG 
TGCAAGCTGC CTTCCTCGGA GCCGCTCAGC AGGGCCTTTG TTTACAGGGA TGTGACTGAC 
TGTACCAGCG TCACTGGCAC TTTCAAATTG TTCTTAATCA TCCAGATGGT CCTAAACCTG 
GTCTGTGGCC TCGTGTGCTT GCTGGCTTGC TTTGTGATGT GGAAACACAG GTACCAGGTC 
TTTTATGTGG GTGTTGGGCT GCGCTCCCTG ATGGCTTCTG ATGGCCAGCT ACCAAAGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001297766.1
CDS	168..590
Translation	MLCVSYQVDERTCVQFSMKVFYFLLSALGLMVCMLAVAFAAHHYSLLAQFTCETSLDSCQCKLPSSEPLSRAFVYRDVTDCTSVTGTFKLFLIIQMVLNLVCGLVCLLACFVMWKHRYQVFYVGVGLRSLMASDGQLPKA

Target ORF information:

RefSeq Version	NM_001310837.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sarcospan (Sspn), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001310837.1

ORF Insert Sequence:

ATGCTTTGTG TCTCATACCA AGTTGACGAG AGGACATGTG TCCAGTTTTC TATGAAGGTA 
TTCTACTTCC TGTTGAGTGC CCTCGGCCTG ATGGTCTGCA TGCTGGCTGT GGCCTTTGCC 
GCCCACCACT ACTCCCTGCT TGCGCAGTTT ACCTGCGAGA CCTCCCTGGA TTCTTGCCAG 
TGCAAGCTGC CTTCCTCGGA GCCGCTCAGC AGGGCCTTTG TTTACAGGGA TGTGACTGAC 
TGTACCAGCG TCACTGGCAC TTTCAAATTG TTCTTAATCA TCCAGATGGT CCTAAACCTG 
GTCTGTGGCC TCGTGTGCTT GCTGGCTTGC TTTGTGATGT GGAAACACAG GTACCAGGTC 
TTTTATGTGG GTGTTGGGCT GCGCTCCCTG ATGGCTTCTG ATGGCCAGCT ACCAAAGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sspn ( XM_006506916.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006506979.1 Mus musculus sarcospan (Sspn), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu37791
Clone ID Related Accession (Same CDS sequence)	XM_006506916.4 , XM_006506916.3
Accession Version	XM_006506916.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	sarcospan isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006506916.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGAAG TCTGCGTGGT GGCTTACCTT GGACTGTTCA TGCTTTGTGT CTCATACCAA 
GTTGACGAGA GGACATGTGT CCAGTTTTCT ATGAAGGTAT TCTACTTCCT GTTGAGTGCC 
CTCGGCCTGA TGGTCTGCAT GCTGGCTGTG GCCTTTGCCG CCCACCACTA CTCCCTGCTT 
GCGCAGTTTA CCTGCGAGAC CTCCCTGGAT TCTTGCCAGT GCAAGCTGCC TTCCTCGGAG 
CCGCTCAGCA GGGCCTTTGT TTACAGGGAT GTGACTGACT GTACCAGCGT CACTGGCACT 
TTCAAATTGT TCTTAATCAT CCAGATGGTC CTAAACCTGG TCTGTGGCCT CGTGTGCTTG 
CTGGCTTGCT TTGTGATGTG GAAACACAGG TACCAGGTCT TTTATGTGGG TGTTGGGCTG 
CGCTCCCTGA TGGCTTCTGA TGGCCAGCTA CCAAAGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006506979.1
CDS	91..552
Translation	MTEVCVVAYLGLFMLCVSYQVDERTCVQFSMKVFYFLLSALGLMVCMLAVAFAAHHYSLLAQFTCETSLDSCQCKLPSSEPLSRAFVYRDVTDCTSVTGTFKLFLIIQMVLNLVCGLVCLLACFVMWKHRYQVFYVGVGLRSLMASDGQLPKA

Target ORF information:

RefSeq Version	XM_006506916.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sarcospan (Sspn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006506916.3

ORF Insert Sequence:

ATGACAGAAG TCTGCGTGGT GGCTTACCTT GGACTGTTCA TGCTTTGTGT CTCATACCAA 
GTTGACGAGA GGACATGTGT CCAGTTTTCT ATGAAGGTAT TCTACTTCCT GTTGAGTGCC 
CTCGGCCTGA TGGTCTGCAT GCTGGCTGTG GCCTTTGCCG CCCACCACTA CTCCCTGCTT 
GCGCAGTTTA CCTGCGAGAC CTCCCTGGAT TCTTGCCAGT GCAAGCTGCC TTCCTCGGAG 
CCGCTCAGCA GGGCCTTTGT TTACAGGGAT GTGACTGACT GTACCAGCGT CACTGGCACT 
TTCAAATTGT TCTTAATCAT CCAGATGGTC CTAAACCTGG TCTGTGGCCT CGTGTGCTTG 
CTGGCTTGCT TTGTGATGTG GAAACACAGG TACCAGGTCT TTTATGTGGG TGTTGGGCTG 
CGCTCCCTGA TGGCTTCTGA TGGCCAGCTA CCAAAGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sspn ( NM_010656.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034786.1 Mus musculus sarcospan (Sspn), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu06550
Clone ID Related Accession (Same CDS sequence)	NM_010656.2 , NM_010656.3
Accession Version	NM_010656.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Mus musculus(house mouse)
Product	sarcospan isoform 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC144936.4. On Nov 17, 2006 this sequence version replaced NM_010656.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U02487.1, AK148179.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGGGCGCA AGCCGAGCCC CAGGGCTCAG GAGCTGCCTG AGGAGGAGGC GCGGACCTGC 
TGTGGCTGCC GCTTCCCGCT GCTGCTGGCA CTGCTGCAGC TGGCGTTGGG CATCGCGGTG 
ACCGTGCTGG GCTTCCTCAT GGCGAGCATC AGCCCCTCCC TGCTAGTCAG AGATACTCCG 
TTCTGGGCTG GGATCATTGT CTGCGTGGTG GCTTACCTTG GACTGTTCAT GCTTTGTGTC 
TCATACCAAG TTGACGAGAG GACATGTGTC CAGTTTTCTA TGAAGGTATT CTACTTCCTG 
TTGAGTGCCC TCGGCCTGAT GGTCTGCATG CTGGCTGTGG CCTTTGCCGC CCACCACTAC 
TCCCTGCTTG CGCAGTTTAC CTGCGAGACC TCCCTGGATT CTTGCCAGTG CAAGCTGCCT 
TCCTCGGAGC CGCTCAGCAG GGCCTTTGTT TACAGGGATG TGACTGACTG TACCAGCGTC 
ACTGGCACTT TCAAATTGTT CTTAATCATC CAGATGGTCC TAAACCTGGT CTGTGGCCTC 
GTGTGCTTGC TGGCTTGCTT TGTGATGTGG AAACACAGGT ACCAGGTCTT TTATGTGGGT 
GTTGGGCTGC GCTCCCTGAT GGCTTCTGAT GGCCAGCTAC CAAAGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034786.1
CDS	28..678
Translation	MGRKPSPRAQELPEEEARTCCGCRFPLLLALLQLALGIAVTVLGFLMASISPSLLVRDTPFWAGIIVCVVAYLGLFMLCVSYQVDERTCVQFSMKVFYFLLSALGLMVCMLAVAFAAHHYSLLAQFTCETSLDSCQCKLPSSEPLSRAFVYRDVTDCTSVTGTFKLFLIIQMVLNLVCGLVCLLACFVMWKHRYQVFYVGVGLRSLMASDGQLPKA

Target ORF information:

RefSeq Version	NM_010656.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sarcospan (Sspn), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010656.2

ORF Insert Sequence:

ATGGGGCGCA AGCCGAGCCC CAGGGCTCAG GAGCTGCCTG AGGAGGAGGC GCGGACCTGC 
TGTGGCTGCC GCTTCCCGCT GCTGCTGGCA CTGCTGCAGC TGGCGTTGGG CATCGCGGTG 
ACCGTGCTGG GCTTCCTCAT GGCGAGCATC AGCCCCTCCC TGCTAGTCAG AGATACTCCG 
TTCTGGGCTG GGATCATTGT CTGCGTGGTG GCTTACCTTG GACTGTTCAT GCTTTGTGTC 
TCATACCAAG TTGACGAGAG GACATGTGTC CAGTTTTCTA TGAAGGTATT CTACTTCCTG 
TTGAGTGCCC TCGGCCTGAT GGTCTGCATG CTGGCTGTGG CCTTTGCCGC CCACCACTAC 
TCCCTGCTTG CGCAGTTTAC CTGCGAGACC TCCCTGGATT CTTGCCAGTG CAAGCTGCCT 
TCCTCGGAGC CGCTCAGCAG GGCCTTTGTT TACAGGGATG TGACTGACTG TACCAGCGTC 
ACTGGCACTT TCAAATTGTT CTTAATCATC CAGATGGTCC TAAACCTGGT CTGTGGCCTC 
GTGTGCTTGC TGGCTTGCTT TGTGATGTGG AAACACAGGT ACCAGGTCTT TTATGTGGGT 
GTTGGGCTGC GCTCCCTGAT GGCTTCTGAT GGCCAGCTAC CAAAGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sspn ( NM_010656.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034786.1 Mus musculus sarcospan (Sspn), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu06550
Clone ID Related Accession (Same CDS sequence)	NM_010656.2 , NM_010656.3
Accession Version	NM_010656.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Mus musculus(house mouse)
Product	sarcospan isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC144936.4. On Mar 19, 2018 this sequence version replaced NM_010656.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U02487.1, AK148179.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCGCA AGCCGAGCCC CAGGGCTCAG GAGCTGCCTG AGGAGGAGGC GCGGACCTGC 
TGTGGCTGCC GCTTCCCGCT GCTGCTGGCA CTGCTGCAGC TGGCGTTGGG CATCGCGGTG 
ACCGTGCTGG GCTTCCTCAT GGCGAGCATC AGCCCCTCCC TGCTAGTCAG AGATACTCCG 
TTCTGGGCTG GGATCATTGT CTGCGTGGTG GCTTACCTTG GACTGTTCAT GCTTTGTGTC 
TCATACCAAG TTGACGAGAG GACATGTGTC CAGTTTTCTA TGAAGGTATT CTACTTCCTG 
TTGAGTGCCC TCGGCCTGAT GGTCTGCATG CTGGCTGTGG CCTTTGCCGC CCACCACTAC 
TCCCTGCTTG CGCAGTTTAC CTGCGAGACC TCCCTGGATT CTTGCCAGTG CAAGCTGCCT 
TCCTCGGAGC CGCTCAGCAG GGCCTTTGTT TACAGGGATG TGACTGACTG TACCAGCGTC 
ACTGGCACTT TCAAATTGTT CTTAATCATC CAGATGGTCC TAAACCTGGT CTGTGGCCTC 
GTGTGCTTGC TGGCTTGCTT TGTGATGTGG AAACACAGGT ACCAGGTCTT TTATGTGGGT 
GTTGGGCTGC GCTCCCTGAT GGCTTCTGAT GGCCAGCTAC CAAAGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034786.1
CDS	53..703
Translation	MGRKPSPRAQELPEEEARTCCGCRFPLLLALLQLALGIAVTVLGFLMASISPSLLVRDTPFWAGIIVCVVAYLGLFMLCVSYQVDERTCVQFSMKVFYFLLSALGLMVCMLAVAFAAHHYSLLAQFTCETSLDSCQCKLPSSEPLSRAFVYRDVTDCTSVTGTFKLFLIIQMVLNLVCGLVCLLACFVMWKHRYQVFYVGVGLRSLMASDGQLPKA

Target ORF information:

RefSeq Version	NM_010656.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sarcospan (Sspn), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010656.3

ORF Insert Sequence:

ATGGGGCGCA AGCCGAGCCC CAGGGCTCAG GAGCTGCCTG AGGAGGAGGC GCGGACCTGC 
TGTGGCTGCC GCTTCCCGCT GCTGCTGGCA CTGCTGCAGC TGGCGTTGGG CATCGCGGTG 
ACCGTGCTGG GCTTCCTCAT GGCGAGCATC AGCCCCTCCC TGCTAGTCAG AGATACTCCG 
TTCTGGGCTG GGATCATTGT CTGCGTGGTG GCTTACCTTG GACTGTTCAT GCTTTGTGTC 
TCATACCAAG TTGACGAGAG GACATGTGTC CAGTTTTCTA TGAAGGTATT CTACTTCCTG 
TTGAGTGCCC TCGGCCTGAT GGTCTGCATG CTGGCTGTGG CCTTTGCCGC CCACCACTAC 
TCCCTGCTTG CGCAGTTTAC CTGCGAGACC TCCCTGGATT CTTGCCAGTG CAAGCTGCCT 
TCCTCGGAGC CGCTCAGCAG GGCCTTTGTT TACAGGGATG TGACTGACTG TACCAGCGTC 
ACTGGCACTT TCAAATTGTT CTTAATCATC CAGATGGTCC TAAACCTGGT CTGTGGCCTC 
GTGTGCTTGC TGGCTTGCTT TGTGATGTGG AAACACAGGT ACCAGGTCTT TTATGTGGGT 
GTTGGGCTGC GCTCCCTGAT GGCTTCTGAT GGCCAGCTAC CAAAGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sspn ( XM_006506916.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006506979.1 Mus musculus sarcospan (Sspn), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu37791
Clone ID Related Accession (Same CDS sequence)	XM_006506916.4 , XM_006506916.3
Accession Version	XM_006506916.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	sarcospan isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006506916.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGAAG TCTGCGTGGT GGCTTACCTT GGACTGTTCA TGCTTTGTGT CTCATACCAA 
GTTGACGAGA GGACATGTGT CCAGTTTTCT ATGAAGGTAT TCTACTTCCT GTTGAGTGCC 
CTCGGCCTGA TGGTCTGCAT GCTGGCTGTG GCCTTTGCCG CCCACCACTA CTCCCTGCTT 
GCGCAGTTTA CCTGCGAGAC CTCCCTGGAT TCTTGCCAGT GCAAGCTGCC TTCCTCGGAG 
CCGCTCAGCA GGGCCTTTGT TTACAGGGAT GTGACTGACT GTACCAGCGT CACTGGCACT 
TTCAAATTGT TCTTAATCAT CCAGATGGTC CTAAACCTGG TCTGTGGCCT CGTGTGCTTG 
CTGGCTTGCT TTGTGATGTG GAAACACAGG TACCAGGTCT TTTATGTGGG TGTTGGGCTG 
CGCTCCCTGA TGGCTTCTGA TGGCCAGCTA CCAAAGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006506979.1
CDS	86..547
Translation	MTEVCVVAYLGLFMLCVSYQVDERTCVQFSMKVFYFLLSALGLMVCMLAVAFAAHHYSLLAQFTCETSLDSCQCKLPSSEPLSRAFVYRDVTDCTSVTGTFKLFLIIQMVLNLVCGLVCLLACFVMWKHRYQVFYVGVGLRSLMASDGQLPKA

Target ORF information:

RefSeq Version	XM_006506916.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sarcospan (Sspn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006506916.4

ORF Insert Sequence:

ATGACAGAAG TCTGCGTGGT GGCTTACCTT GGACTGTTCA TGCTTTGTGT CTCATACCAA 
GTTGACGAGA GGACATGTGT CCAGTTTTCT ATGAAGGTAT TCTACTTCCT GTTGAGTGCC 
CTCGGCCTGA TGGTCTGCAT GCTGGCTGTG GCCTTTGCCG CCCACCACTA CTCCCTGCTT 
GCGCAGTTTA CCTGCGAGAC CTCCCTGGAT TCTTGCCAGT GCAAGCTGCC TTCCTCGGAG 
CCGCTCAGCA GGGCCTTTGT TTACAGGGAT GTGACTGACT GTACCAGCGT CACTGGCACT 
TTCAAATTGT TCTTAATCAT CCAGATGGTC CTAAACCTGG TCTGTGGCCT CGTGTGCTTG 
CTGGCTTGCT TTGTGATGTG GAAACACAGG TACCAGGTCT TTTATGTGGG TGTTGGGCTG 
CGCTCCCTGA TGGCTTCTGA TGGCCAGCTA CCAAAGGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nanospan, an alternatively spliced isoform of sarcospan, localizes to the sarcoplasmic reticulum in skeletal muscle and is absent in limb girdle muscular dystrophy 2F.
Skeletal muscle7(1)11(2017 Jun)
Peter AK,Miller G,Capote J,DiFranco M,Solares-P?rez A,Wang EL,Heighway J,Coral-V?zquez RM,Vergara J,Crosbie-Watson RH

High levels of sarcospan are well tolerated and act as a sarcolemmal stabilizer to address skeletal muscle and pulmonary dysfunction in DMD.
Human molecular genetics25(24)5395-5406(2016 12)
Gibbs EM,Marshall JL,Ma E,Nguyen TM,Hong G,Lam JS,Spencer MJ,Crosbie-Watson RH

Decreased expression levels of Ifi genes is associated to the increased resistance to spontaneous arthritis disease in mice deficiency of IL-1RA.
BMC immunology17(1)25(2016 08)
Liu X,Jiao Y,Cao Y,Deng N,Ma Y,Hasty KA,Kang A,Chen H,Stuart JM,Gu W

Sarcospan Regulates Cardiac Isoproterenol Response and Prevents Duchenne Muscular Dystrophy-Associated Cardiomyopathy.
Journal of the American Heart Association4(12)(2015 Dec)
Parvatiyar MS,Marshall JL,Nguyen RT,Jordan MC,Richardson VA,Roos KP,Crosbie-Watson RH

Sarcospan integration into laminin-binding adhesion complexes that ameliorate muscular dystrophy requires utrophin and ?7 integrin.
Human molecular genetics24(7)2011-22(2015 Apr)
Marshall JL,Oh J,Chou E,Lee JA,Holmberg J,Burkin DJ,Crosbie-Watson RH