Kcnu1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kcnu1
Entrez Gene ID	16532
Full Name	potassium channel, subfamily U, member 1
Synonyms	Kcnma3,Slo3,mSlo3
General protein information	Preferred Names potassium channel, subfamily U, member 1 Names potassium channel subfamily U member 1 KSper calcium-activated potassium channel subunit alpha-3 calcium-activated potassium channel, subfamily M subunit alpha-3 pH-sensitive maxi potassium channel pore-forming subunit of the sperm-specific alkalization activated K(+) current potassium large conductance pH-sensitive channel, subfamily M, alpha member 3 slowpoke homolog 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 A2

mRNA and Protein(s)

mRNA	Protein	Name
XM_017312583.1	XP_017168072.1	potassium channel subfamily U member 1 isoform X4
XM_017312584.1	XP_017168073.1	potassium channel subfamily U member 1 isoform X5
XM_017312581.1	XP_017168070.1	potassium channel subfamily U member 1 isoform X2
XM_017312582.1	XP_017168071.1	potassium channel subfamily U member 1 isoform X3
NM_008432.3	NP_032458.3	potassium channel subfamily U member 1
XM_006509024.2	XP_006509087.1	potassium channel subfamily U member 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	KCNU1	NP_001027006.2
	KCNU1	XP_001088318.1
Canis lupus familiaris (dog)	KCNU1	XP_539971.4
Bos taurus (cattle)	KCNU1	NP_001156721.1
Mus musculus (house mouse)	Kcnu1	NP_032458.3
Rattus norvegicus (Norway rat)	Kcnu1	XP_006253398.1
Pan troglodytes (chimpanzee)	KCNU1	XP_519704.4
Gallus gallus (chicken)	LOC100857499	XP_003642598.2

Gene Ontology
Bibliography

Related articles in PubMed

Transient exposure to calcium ionophore enables in vitro fertilization in sterile mouse models.
Navarrete FA, Alvau A, Lee HC, Levin LR, Buck J, Leon PM, Santi CM, Krapf D, Mager J, Fissore RA, Salicioni AM, Darszon A, Visconti PE
Scientific reports633589(2016 Sep)

Src Kinase Is the Connecting Player between Protein Kinase A (PKA) Activation and Hyperpolarization through SLO3 Potassium Channel Regulation in Mouse Sperm.
Stival C, La Spina FA, Bar? Graf C, Arcelay E, Arranz SE, Ferreira JJ, Le Grand S, Dzikunu VA, Santi CM, Visconti PE, Buffone MG, Krapf D
The Journal of biological chemistry290(30)18855-64(2015 Jul)

Flow cytometry analysis reveals that only a subpopulation of mouse sperm undergoes hyperpolarization during capacitation.
Escoffier J, Navarrete F, Haddad D, Santi CM, Darszon A, Visconti PE
Biology of reproduction92(5)121(2015 May)

SLO3 auxiliary subunit LRRC52 controls gating of sperm KSPER currents and is critical for normal fertility.
Zeng XH, Yang C, Xia XM, Liu M, Lingle CJ
Proceedings of the National Academy of Sciences of the United States of America112(8)2599-604(2015 Feb)

SLO3 K+ channels control calcium entry through CATSPER channels in sperm.
Ch?vez JC, Ferreira JJ, Butler A, De La Vega Beltr?n JL, Trevi?o CL, Darszon A, Salkoff L, Santi CM
The Journal of biological chemistry289(46)32266-75(2014 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Considering that capacitation-induced hyperpolarization is mediated by SLO3, we evaluated the action of cSrc inhibitors on the heterologously expressed SLO3 channel.
Title: Src Kinase Is the Connecting Player between Protein Kinase A (PKA) Activation and Hyperpolarization through SLO3 Potassium Channel Regulation in Mouse Sperm.

These results establish a critical role of LRRC52 in KSPER channels and demonstrate that loss of a non-pore-forming auxiliary subunit results in severe fertility impairment.
Title: SLO3 auxiliary subunit LRRC52 controls gating of sperm KSPER currents and is critical for normal fertility.

SLO3 K+ channels have a role in controlling calcium entry through CATSPER channels in sperm
Title: SLO3 K+ channels control calcium entry through CATSPER channels in sperm.

Using mice lacking both SLO3 and CATSPER1 subunits, the results show conclusively that the voltage-activated outward current present in Slo3 (-/-) sperm is abolished when CATSPER is also deleted.
Title: Simultaneous knockout of Slo3 and CatSper1 abolishes all alkalization- and voltage-activated current in mouse spermatozoa.

SLO3-dependent hyperpolarization
Title: Ion permeabilities in mouse sperm reveal an external trigger for SLO3-dependent hyperpolarization.

The following Kcnu1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kcnu1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu82357	XM_017312584.1 Latest version!	Mus musculus potassium channel, subfamily U, member 1 (Kcnu1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu82355	XM_017312581.1 Latest version!	Mus musculus potassium channel, subfamily U, member 1 (Kcnu1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu82356	XM_017312582.1 Latest version!	Mus musculus potassium channel, subfamily U, member 1 (Kcnu1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu19323	NM_008432.3 Latest version!	Mus musculus potassium channel, subfamily U, member 1 (Kcnu1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu40570	XM_006509024.2 Latest version!	Mus musculus potassium channel, subfamily U, member 1 (Kcnu1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu68043	XM_017312583.1 Latest version!	Mus musculus potassium channel, subfamily U, member 1 (Kcnu1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kcnu1 ( XM_017312584.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168073.1 Mus musculus potassium channel, subfamily U, member 1 (Kcnu1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu82357
Clone ID Related Accession (Same CDS sequence)	XM_017312584.1
Accession Version	XM_017312584.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily U member 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAAA CATTGCTAGA CAGTTTAAAT CAGAAGGAGT TGACGGAAAC GTCATGTACA 
ATCGAAATCC AGGCAGCGTT CATTCTTTCC TCCTTGGCGA CTTTCTTCGG GGGACTCATC 
ATCTTATTCC TTTTCAGAAT AGCCTTGAAA AGCTCAAGAA GTTGGAAATA CGTCAAGGGG 
CCAAGAGGAC TCTTGGAACT ATTCTCATCA CGTAGAATCG AGGCTAATCC TTTGAGGAAA 
CTTTACTTTC ATGGAGTATT TCGTCAGCGC ATCGAAATGC TGCTTTCTGC ACAGACCGTC 
GTGGGGCAAG TGTTGGTGAT CCTTGTCTTT GTACTAAGCA TCGGGTCTCT TGTGATCTAT 
TTCATCAATT CAATGGATCC TGTTCGAAGG TGTTCTTCAT ATGAAGACAA AATTGTCCAT 
GTGGATTTGA GTTTCAACGC TTTCTTTAGC TTCTATTTTG GGTTGAGGTT TTGGGCAGCT 
GAAGACAAGA TCAAGTTCTG GTTGGAGATG AATTCAATTG TAGACATTTT TACCATCCCG 
CCAACCTTTA TTTCTTATTA TTTGAAGAGT AATTGGCTAG GTTTGAGATT TCTAAGAGCT 
CTGCGGTTGC TCGAACTCCC TAAAATCTTA CAGATCCTAC AAGTCATCAA GACCAGCAAT 
TCAGTGAAGC TTTCCAAACT GTTGTCAATA GTTATCAGTA CCTGGTTCAC GGCAGCAGGA 
TTCCTTCACC TGACCTTTCC TTTGCCATCT CTCGTTGGAC AGGTGGAAAA TTCTGGTGAC 
CCCTGGCTCA ACGGAAGAAA CTCACAGACT ATGTCATACT TTGAGTCTAT TTATCTGGTG 
ACAGCAACAA TGTCAACTGT TGGCTTTGGG GACGTGGTGG CCAAGACATC CCTAGGACGG 
ATTTTCATTG TTTTCTTCAC CCTTGGGAGT TTGATACTAT TTGCAAACTA CATTCCAGAA 
ATGGTGGAGC TCTTTTCTAC CAGGAAGAAA TACACCAAGC CCTACGAAGC AGTCAAAGGA 
AAAAAGTTCA TCGTGGTCTG TGGAAACATC ACAGTTGACA GTGTTACTGC TTTCCTGAGG 
AATTTTCTCC ACTGGAAGTC CGGGGAAATC AATATTGAGA TCGTATTCCT TGGAGAGACT 
CTCCCTTGCT TGGAACTGGA GACCTTACTG AAGTGCCACA CATCCTGTAC CAACTTCGTA 
TGCGGCACCG CACTGAAGTT CGAGGATCTG AAGCGAGTTG CAGTGGAGAA CTCGGAGGCG 
TGCCTGATTC TAGCCAACCA TTTCTGTAGT GACTTACATG ACGAAGACAA CTCAAACATT 
ATGAGGGTGC TCTCGATCAA GAACTATTAT CCACAGACCA GAGTCATCAT TCAGATACTT 
CAGTCTCAAA ACAAGGTTTT CCTGTCAAAA ATCCCCAACT GGGACTGGAG TGCTGGAGAC 
AATATCCTCT GCTTTGCAGA GCTAAAGCTC GGATTTATCG CCCAAGGCTG CTTGGTGCCA 
GGGCTGTGCA CCTTTCTCAC GACTCTGTTC ATTGAACAAA ACCAAAAGGT TTTTCCTAAA 
CATCCCTGGC AAAAACATTT CTTGAATGGC TTGAAGAACA AGATTCTGAC ACAGCGCCTC 
TCTAACGACT TCGTGGGGAT GACATTTCCC CAGGTCTCCC GGCTCTGCTT TGTGAAGCTA 
AATCTCATGC TGATCGCCAT CCAACACAAG CCCTTCTTTC ACAGTTGTTG CACTCTGATA 
CTAAACCCAT CATCCCAAGT GAGGCTGAAT AAGGACACCT TAGGGTTCTT CATTGCGGAC 
TCCTCCAAAG CCGTCAAAAG GGCTTTCTTT TACTGTTCCA ACTGTCACAG CGATGTGTGC 
AATCCTGAGC TAATTGGAAA GTGTAACTGT AAAATCAAGA GCCGACAACA ACTCATAGCA 
CCGACCATCA TGGTGATGAA AAGCAGCTTG ACCGATTTCA CCACTTCTTC ACACATCCAC 
GCTTCTATGT CAACAGAAAT TCACACTTGT TTTTCAAGAG AACAGCCTAG TTTGATCACC 
ATTACAACCA ACAGACCAAC GACAAACGAC ACAGTGGATG ATACCGACAT GCTGGACAGC 
AGTGGCATGT TTCACTGGTG CAGAGCAATG CCCTTGGACA AGGTGGTTCT GGAATGGAAT 
GCCCAGATGT TTAAAAACTG GACTTTGAAC TTGGTTCTTC TTGTACAAAG AAACGAAGTG 
AGAAGGCAAA ACACGAGTTT CAGAACCACA TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168073.1
CDS	33..2288
Translation	MSQTLLDSLNQKELTETSCTIEIQAAFILSSLATFFGGLIILFLFRIALKSSRSWKYVKGPRGLLELFSSRRIEANPLRKLYFHGVFRQRIEMLLSAQTVVGQVLVILVFVLSIGSLVIYFINSMDPVRRCSSYEDKIVHVDLSFNAFFSFYFGLRFWAAEDKIKFWLEMNSIVDIFTIPPTFISYYLKSNWLGLRFLRALRLLELPKILQILQVIKTSNSVKLSKLLSIVISTWFTAAGFLHLTFPLPSLVGQVENSGDPWLNGRNSQTMSYFESIYLVTATMSTVGFGDVVAKTSLGRIFIVFFTLGSLILFANYIPEMVELFSTRKKYTKPYEAVKGKKFIVVCGNITVDSVTAFLRNFLHWKSGEINIEIVFLGETLPCLELETLLKCHTSCTNFVCGTALKFEDLKRVAVENSEACLILANHFCSDLHDEDNSNIMRVLSIKNYYPQTRVIIQILQSQNKVFLSKIPNWDWSAGDNILCFAELKLGFIAQGCLVPGLCTFLTTLFIEQNQKVFPKHPWQKHFLNGLKNKILTQRLSNDFVGMTFPQVSRLCFVKLNLMLIAIQHKPFFHSCCTLILNPSSQVRLNKDTLGFFIADSSKAVKRAFFYCSNCHSDVCNPELIGKCNCKIKSRQQLIAPTIMVMKSSLTDFTTSSHIHASMSTEIHTCFSREQPSLITITTNRPTTNDTVDDTDMLDSSGMFHWCRAMPLDKVVLEWNAQMFKNWTLNLVLLVQRNEVRRQNTSFRTTL

Target ORF information:

RefSeq Version	XM_017312584.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily U, member 1 (Kcnu1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312584.1

ORF Insert Sequence:

ATGTCTCAAA CATTGCTAGA CAGTTTAAAT CAGAAGGAGT TGACGGAAAC GTCATGTACA 
ATCGAAATCC AGGCAGCGTT CATTCTTTCC TCCTTGGCGA CTTTCTTCGG GGGACTCATC 
ATCTTATTCC TTTTCAGAAT AGCCTTGAAA AGCTCAAGAA GTTGGAAATA CGTCAAGGGG 
CCAAGAGGAC TCTTGGAACT ATTCTCATCA CGTAGAATCG AGGCTAATCC TTTGAGGAAA 
CTTTACTTTC ATGGAGTATT TCGTCAGCGC ATCGAAATGC TGCTTTCTGC ACAGACCGTC 
GTGGGGCAAG TGTTGGTGAT CCTTGTCTTT GTACTAAGCA TCGGGTCTCT TGTGATCTAT 
TTCATCAATT CAATGGATCC TGTTCGAAGG TGTTCTTCAT ATGAAGACAA AATTGTCCAT 
GTGGATTTGA GTTTCAACGC TTTCTTTAGC TTCTATTTTG GGTTGAGGTT TTGGGCAGCT 
GAAGACAAGA TCAAGTTCTG GTTGGAGATG AATTCAATTG TAGACATTTT TACCATCCCG 
CCAACCTTTA TTTCTTATTA TTTGAAGAGT AATTGGCTAG GTTTGAGATT TCTAAGAGCT 
CTGCGGTTGC TCGAACTCCC TAAAATCTTA CAGATCCTAC AAGTCATCAA GACCAGCAAT 
TCAGTGAAGC TTTCCAAACT GTTGTCAATA GTTATCAGTA CCTGGTTCAC GGCAGCAGGA 
TTCCTTCACC TGACCTTTCC TTTGCCATCT CTCGTTGGAC AGGTGGAAAA TTCTGGTGAC 
CCCTGGCTCA ACGGAAGAAA CTCACAGACT ATGTCATACT TTGAGTCTAT TTATCTGGTG 
ACAGCAACAA TGTCAACTGT TGGCTTTGGG GACGTGGTGG CCAAGACATC CCTAGGACGG 
ATTTTCATTG TTTTCTTCAC CCTTGGGAGT TTGATACTAT TTGCAAACTA CATTCCAGAA 
ATGGTGGAGC TCTTTTCTAC CAGGAAGAAA TACACCAAGC CCTACGAAGC AGTCAAAGGA 
AAAAAGTTCA TCGTGGTCTG TGGAAACATC ACAGTTGACA GTGTTACTGC TTTCCTGAGG 
AATTTTCTCC ACTGGAAGTC CGGGGAAATC AATATTGAGA TCGTATTCCT TGGAGAGACT 
CTCCCTTGCT TGGAACTGGA GACCTTACTG AAGTGCCACA CATCCTGTAC CAACTTCGTA 
TGCGGCACCG CACTGAAGTT CGAGGATCTG AAGCGAGTTG CAGTGGAGAA CTCGGAGGCG 
TGCCTGATTC TAGCCAACCA TTTCTGTAGT GACTTACATG ACGAAGACAA CTCAAACATT 
ATGAGGGTGC TCTCGATCAA GAACTATTAT CCACAGACCA GAGTCATCAT TCAGATACTT 
CAGTCTCAAA ACAAGGTTTT CCTGTCAAAA ATCCCCAACT GGGACTGGAG TGCTGGAGAC 
AATATCCTCT GCTTTGCAGA GCTAAAGCTC GGATTTATCG CCCAAGGCTG CTTGGTGCCA 
GGGCTGTGCA CCTTTCTCAC GACTCTGTTC ATTGAACAAA ACCAAAAGGT TTTTCCTAAA 
CATCCCTGGC AAAAACATTT CTTGAATGGC TTGAAGAACA AGATTCTGAC ACAGCGCCTC 
TCTAACGACT TCGTGGGGAT GACATTTCCC CAGGTCTCCC GGCTCTGCTT TGTGAAGCTA 
AATCTCATGC TGATCGCCAT CCAACACAAG CCCTTCTTTC ACAGTTGTTG CACTCTGATA 
CTAAACCCAT CATCCCAAGT GAGGCTGAAT AAGGACACCT TAGGGTTCTT CATTGCGGAC 
TCCTCCAAAG CCGTCAAAAG GGCTTTCTTT TACTGTTCCA ACTGTCACAG CGATGTGTGC 
AATCCTGAGC TAATTGGAAA GTGTAACTGT AAAATCAAGA GCCGACAACA ACTCATAGCA 
CCGACCATCA TGGTGATGAA AAGCAGCTTG ACCGATTTCA CCACTTCTTC ACACATCCAC 
GCTTCTATGT CAACAGAAAT TCACACTTGT TTTTCAAGAG AACAGCCTAG TTTGATCACC 
ATTACAACCA ACAGACCAAC GACAAACGAC ACAGTGGATG ATACCGACAT GCTGGACAGC 
AGTGGCATGT TTCACTGGTG CAGAGCAATG CCCTTGGACA AGGTGGTTCT GGAATGGAAT 
GCCCAGATGT TTAAAAACTG GACTTTGAAC TTGGTTCTTC TTGTACAAAG AAACGAAGTG 
AGAAGGCAAA ACACGAGTTT CAGAACCACA TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnu1 ( XM_017312581.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168070.1 Mus musculus potassium channel, subfamily U, member 1 (Kcnu1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu82355
Clone ID Related Accession (Same CDS sequence)	XM_017312581.1
Accession Version	XM_017312581.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily U member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAAA CATTGCTAGA CAGTTTAAAT CAGAAGGAGT TGACGGAAAC GTCATGTACA 
ATCGAAATCC AGGCAGCGTT CATTCTTTCC TCCTTGGCGA CTTTCTTCGG GGGACTCATC 
ATCTTATTCC TTTTCAGAAT AGCCTTGAAA AGCTCAAGAA GTTGGAAATA CGTCAAGGGG 
CCAAGAGGAC TCTTGGAACT ATTCTCATCA CGTAGAATCG AGGCTAATCC TTTGAGGAAA 
CTTTACTTTC ATGGAGTATT TCGTCAGCGC ATCGAAATGC TGCTTTCTGC ACAGACCGTC 
GTGGGGCAAG TGTTGGTGAT CCTTGTCTTT GTACTAAGCA TCGGGTCTCT TGTGATCTAT 
TTCATCAATT CAATGGATCC TGTTCGAAGG TGTTCTTCAT ATGAAGACAA AATTGTCCAT 
GTGGATTTGA GTTTCAACGC TTTCTTTAGC TTCTATTTTG GGTTGAGGTT TTGGGCAGCT 
GAAGACAAGA TCAAGTTCTG GTTGGAGATG AATTCAATTG TAGACATTTT TACCATCCCG 
CCAACCTTTA TTTCTTATTA TTTGAAGAGT AATTGGCTAG GTTTGAGATT TCTAAGAGCT 
CTGCGGTTGC TCGAACTCCC TAAAATCTTA CAGATCCTAC AAGTCATCAA GACCAGCAAT 
TCAGTGAAGC TTTCCAAACT GTTGTCAATA GTTATCAGTA CCTGGTTCAC GGCAGCAGGA 
TTCCTTCACC TGACCTTTCC TTTGCCATCT CTCGTTGGAC AGGTGGAAAA TTCTGGTGAC 
CCCTGGCTCA ACGGAAGAAA CTCACAGACT ATGTCATACT TTGAGTCTAT TTATCTGGTG 
ACAGCAACAA TGTCAACTGT TGGCTTTGGG GACGTGGTGG CCAAGACATC CCTAGGACGG 
ATTTTCATTG TTTTCTTCAC CCTTGGGAGT TTGATACTAT TTGCAAACTA CATTCCAGAA 
ATGGTGGAGC TCTTTTCTAC CAGGAAGAAA TACACCAAGC CCTACGAAGC AGTCAAAGGA 
AAAAAGTTCA TCGTGGTCTG TGGAAACATC ACAGTTGACA GTGTTACTGC TTTCCTGAGG 
AATTTTCTCC ACTGGAAGTC CGGGGAAATC AATATTGAGA TCGTATTCCT TGGAGAGACT 
CTCCCTTGCT TGGAACTGGA GACCTTACTG AAGTGCCACA CATCCTGTAC CAACTTCGTA 
TGCGGCACCG CACTGAAGTT CGAGGATCTG AAGCGAGTTG CAGTGGAGAA CTCGGAGGCG 
TGCCTGATTC TAGCCAACCA TTTCTGTAGT GACTTACATG ACGAAGACAA CTCAAACATT 
ATGAGGGTGC TCTCGATCAA GAACTATTAT CCACAGACCA GAGTCATCAT TCAGATACTT 
CAGTCTCAAA ACAAGGTTTT CCTGTCAAAA ATCCCCAACT GGGACTGGAG TGCTGGAGAC 
AATATCCTCT GCTTTGCAGA GCTAAAGCTC GGATTTATCG CCCAAGGCTG CTTGGTGCCA 
GGGCTGTGCA CCTTTCTCAC GACTCTGTTC ATTGAACAAA ACCAAAAGGT TTTTCCTAAA 
CATCCCTGGC AAAAACATTT CTTGAATGGC TTGAAGAACA AGATTCTGAC ACAGCGCCTC 
TCTAACGACT TCGTGGGGAT GACATTTCCC CAGGTCTCCC GGCTCTGCTT TGTGAAGCTA 
AATCTCATGC TGATCGCCAT CCAACACAAG CCCTTCTTTC ACAGTTGTTG GGCTTTCTTT 
TACTGTTCCA ACTGTCACAG CGATGTGTGC AATCCTGAGC TAATTGGAAA GTGTAACTGT 
AAAATCAAGA GCCGACAACA ACTCATAGCA CCGACCATCA TGGTGATGAA AAGCAGCTTG 
ACCGATTTCA CCACTTCTTC ACACATCCAC GCTTCTATGT CAACAGAAAT TCACACTTGT 
TTTTCAAGAG AACAGCCTAG TTTGATCACC ATTACAACCA ACAGACCAAC GACAAACGAC 
ACAGTGGATG ATACCGACAT GCTGGACAGC AGTGGCATGT TTCACTGGTG CAGAGCAATG 
CCCTTGGACA AGGTGGTTCT GAAACGAAGT GAGAAGGCAA AACACGAGTT TCAGAACCAC 
ATTGTAGTAT GCGTGTTTGG AGATGCCCAA TGTACCCTGG TGGGGCTTCG GAATTTCGTG 
ATGCCCCTGA GAGCCAGCAA CTACACCCGG CAGGAGCTGA AGGACATTGT TTTTATTGGG 
TCTCTGGAGT ACTTCCAGAG AGAATGGCGA TTTCTCCGAA ACTTTCCCAA GATACACATT 
ATGCCTGGAT CTGCACTCTA CATGGGAGAT CTGATTGCAG TCAATGTAGA GCAGTGCTCT 
ATGTGCGTCA TCTTAGCCAC ACCCTACAAG GCACTGAGCA GCCAGATTCT GGTGGACACA 
GAGGCCATCA TGGCCACCCT CAACATCCAG TCCCTGCGGA TCACCAGTCC TACTCCAGGG 
TCTTCAAAGT CAGAAGTAAA GCCATCATCT GCCTTTGATA GTAAAGAAAG GAAGCAAAGA 
TACAAACAGA TCCCCATTCT CACTGAACTG AAGAATCCCT CCAACATCCA CTTTATTGAG 
CAGATGGGCG GACTGGATGG AATGCTCAAA GGGACTAGCT TGCATCTCAG CACTTCTTTC 
TCCACCGGTG CTGTCTTTTC AGACACCTTC TTGGATTCTC TCCTGGCCAC GTCCTTCTAC 
AATTACCATG TCGTGGAATT ACTTCAGATG CTAGTGACTG GAGGCATAAG CTCTGAGATG 
GAACACTATT TGGTTAAGGA GAAGCCCTAT AAGACAACTG ACGACTATGA GGCAATCAAG 
TCTGGGAGGA CGCGGTGTAA GCTGGGACTC CTCTCTTTAG ACCAAACCGT TCTATCAGGC 
ATTAATCCAA GAAAAACCTT TGGACAGCTG TTCTGTGGCT CATTGGATAA TTTCGGGATC 
CTATGTGTCG GCTTATACCG TATGATTGAT GAAGAGGAAC CCAGCCAAGA ACACAAAAGG 
TTTGTGATCA CCAGGCCATC CAATGAGTGC CACCTGCTGC CCTCAGATCT CGTGTTTTGT 
GCCATCCCTT TCAACACCAC CTGTGGCAAA TCAGACAGCA GTCCTTCAAT TCAGGCTCAA 
AACAACTCTA CAAACGCGAC GACGCCATTG GCCCAGGGGT CGAATTTCTT CGATTCGCAC 
CATGCCGACG AGTCCCACGA TCTTTACCCA GTCGACGACA CGGGAGAGAG GTGGTCTCAG 
CACCACCACT CCCGAGTCTA TCCTTTGGAC ACGTTAGATG CCAGTGATAT TGTTCAAGAA 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168070.1
CDS	33..3338
Translation	MSQTLLDSLNQKELTETSCTIEIQAAFILSSLATFFGGLIILFLFRIALKSSRSWKYVKGPRGLLELFSSRRIEANPLRKLYFHGVFRQRIEMLLSAQTVVGQVLVILVFVLSIGSLVIYFINSMDPVRRCSSYEDKIVHVDLSFNAFFSFYFGLRFWAAEDKIKFWLEMNSIVDIFTIPPTFISYYLKSNWLGLRFLRALRLLELPKILQILQVIKTSNSVKLSKLLSIVISTWFTAAGFLHLTFPLPSLVGQVENSGDPWLNGRNSQTMSYFESIYLVTATMSTVGFGDVVAKTSLGRIFIVFFTLGSLILFANYIPEMVELFSTRKKYTKPYEAVKGKKFIVVCGNITVDSVTAFLRNFLHWKSGEINIEIVFLGETLPCLELETLLKCHTSCTNFVCGTALKFEDLKRVAVENSEACLILANHFCSDLHDEDNSNIMRVLSIKNYYPQTRVIIQILQSQNKVFLSKIPNWDWSAGDNILCFAELKLGFIAQGCLVPGLCTFLTTLFIEQNQKVFPKHPWQKHFLNGLKNKILTQRLSNDFVGMTFPQVSRLCFVKLNLMLIAIQHKPFFHSCWAFFYCSNCHSDVCNPELIGKCNCKIKSRQQLIAPTIMVMKSSLTDFTTSSHIHASMSTEIHTCFSREQPSLITITTNRPTTNDTVDDTDMLDSSGMFHWCRAMPLDKVVLKRSEKAKHEFQNHIVVCVFGDAQCTLVGLRNFVMPLRASNYTRQELKDIVFIGSLEYFQREWRFLRNFPKIHIMPGSALYMGDLIAVNVEQCSMCVILATPYKALSSQILVDTEAIMATLNIQSLRITSPTPGSSKSEVKPSSAFDSKERKQRYKQIPILTELKNPSNIHFIEQMGGLDGMLKGTSLHLSTSFSTGAVFSDTFLDSLLATSFYNYHVVELLQMLVTGGISSEMEHYLVKEKPYKTTDDYEAIKSGRTRCKLGLLSLDQTVLSGINPRKTFGQLFCGSLDNFGILCVGLYRMIDEEEPSQEHKRFVITRPSNECHLLPSDLVFCAIPFNTTCGKSDSSPSIQAQNNSTNATTPLAQGSNFFDSHHADESHDLYPVDDTGERWSQHHHSRVYPLDTLDASDIVQEK

Target ORF information:

RefSeq Version	XM_017312581.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily U, member 1 (Kcnu1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312581.1

ORF Insert Sequence:

ATGTCTCAAA CATTGCTAGA CAGTTTAAAT CAGAAGGAGT TGACGGAAAC GTCATGTACA 
ATCGAAATCC AGGCAGCGTT CATTCTTTCC TCCTTGGCGA CTTTCTTCGG GGGACTCATC 
ATCTTATTCC TTTTCAGAAT AGCCTTGAAA AGCTCAAGAA GTTGGAAATA CGTCAAGGGG 
CCAAGAGGAC TCTTGGAACT ATTCTCATCA CGTAGAATCG AGGCTAATCC TTTGAGGAAA 
CTTTACTTTC ATGGAGTATT TCGTCAGCGC ATCGAAATGC TGCTTTCTGC ACAGACCGTC 
GTGGGGCAAG TGTTGGTGAT CCTTGTCTTT GTACTAAGCA TCGGGTCTCT TGTGATCTAT 
TTCATCAATT CAATGGATCC TGTTCGAAGG TGTTCTTCAT ATGAAGACAA AATTGTCCAT 
GTGGATTTGA GTTTCAACGC TTTCTTTAGC TTCTATTTTG GGTTGAGGTT TTGGGCAGCT 
GAAGACAAGA TCAAGTTCTG GTTGGAGATG AATTCAATTG TAGACATTTT TACCATCCCG 
CCAACCTTTA TTTCTTATTA TTTGAAGAGT AATTGGCTAG GTTTGAGATT TCTAAGAGCT 
CTGCGGTTGC TCGAACTCCC TAAAATCTTA CAGATCCTAC AAGTCATCAA GACCAGCAAT 
TCAGTGAAGC TTTCCAAACT GTTGTCAATA GTTATCAGTA CCTGGTTCAC GGCAGCAGGA 
TTCCTTCACC TGACCTTTCC TTTGCCATCT CTCGTTGGAC AGGTGGAAAA TTCTGGTGAC 
CCCTGGCTCA ACGGAAGAAA CTCACAGACT ATGTCATACT TTGAGTCTAT TTATCTGGTG 
ACAGCAACAA TGTCAACTGT TGGCTTTGGG GACGTGGTGG CCAAGACATC CCTAGGACGG 
ATTTTCATTG TTTTCTTCAC CCTTGGGAGT TTGATACTAT TTGCAAACTA CATTCCAGAA 
ATGGTGGAGC TCTTTTCTAC CAGGAAGAAA TACACCAAGC CCTACGAAGC AGTCAAAGGA 
AAAAAGTTCA TCGTGGTCTG TGGAAACATC ACAGTTGACA GTGTTACTGC TTTCCTGAGG 
AATTTTCTCC ACTGGAAGTC CGGGGAAATC AATATTGAGA TCGTATTCCT TGGAGAGACT 
CTCCCTTGCT TGGAACTGGA GACCTTACTG AAGTGCCACA CATCCTGTAC CAACTTCGTA 
TGCGGCACCG CACTGAAGTT CGAGGATCTG AAGCGAGTTG CAGTGGAGAA CTCGGAGGCG 
TGCCTGATTC TAGCCAACCA TTTCTGTAGT GACTTACATG ACGAAGACAA CTCAAACATT 
ATGAGGGTGC TCTCGATCAA GAACTATTAT CCACAGACCA GAGTCATCAT TCAGATACTT 
CAGTCTCAAA ACAAGGTTTT CCTGTCAAAA ATCCCCAACT GGGACTGGAG TGCTGGAGAC 
AATATCCTCT GCTTTGCAGA GCTAAAGCTC GGATTTATCG CCCAAGGCTG CTTGGTGCCA 
GGGCTGTGCA CCTTTCTCAC GACTCTGTTC ATTGAACAAA ACCAAAAGGT TTTTCCTAAA 
CATCCCTGGC AAAAACATTT CTTGAATGGC TTGAAGAACA AGATTCTGAC ACAGCGCCTC 
TCTAACGACT TCGTGGGGAT GACATTTCCC CAGGTCTCCC GGCTCTGCTT TGTGAAGCTA 
AATCTCATGC TGATCGCCAT CCAACACAAG CCCTTCTTTC ACAGTTGTTG GGCTTTCTTT 
TACTGTTCCA ACTGTCACAG CGATGTGTGC AATCCTGAGC TAATTGGAAA GTGTAACTGT 
AAAATCAAGA GCCGACAACA ACTCATAGCA CCGACCATCA TGGTGATGAA AAGCAGCTTG 
ACCGATTTCA CCACTTCTTC ACACATCCAC GCTTCTATGT CAACAGAAAT TCACACTTGT 
TTTTCAAGAG AACAGCCTAG TTTGATCACC ATTACAACCA ACAGACCAAC GACAAACGAC 
ACAGTGGATG ATACCGACAT GCTGGACAGC AGTGGCATGT TTCACTGGTG CAGAGCAATG 
CCCTTGGACA AGGTGGTTCT GAAACGAAGT GAGAAGGCAA AACACGAGTT TCAGAACCAC 
ATTGTAGTAT GCGTGTTTGG AGATGCCCAA TGTACCCTGG TGGGGCTTCG GAATTTCGTG 
ATGCCCCTGA GAGCCAGCAA CTACACCCGG CAGGAGCTGA AGGACATTGT TTTTATTGGG 
TCTCTGGAGT ACTTCCAGAG AGAATGGCGA TTTCTCCGAA ACTTTCCCAA GATACACATT 
ATGCCTGGAT CTGCACTCTA CATGGGAGAT CTGATTGCAG TCAATGTAGA GCAGTGCTCT 
ATGTGCGTCA TCTTAGCCAC ACCCTACAAG GCACTGAGCA GCCAGATTCT GGTGGACACA 
GAGGCCATCA TGGCCACCCT CAACATCCAG TCCCTGCGGA TCACCAGTCC TACTCCAGGG 
TCTTCAAAGT CAGAAGTAAA GCCATCATCT GCCTTTGATA GTAAAGAAAG GAAGCAAAGA 
TACAAACAGA TCCCCATTCT CACTGAACTG AAGAATCCCT CCAACATCCA CTTTATTGAG 
CAGATGGGCG GACTGGATGG AATGCTCAAA GGGACTAGCT TGCATCTCAG CACTTCTTTC 
TCCACCGGTG CTGTCTTTTC AGACACCTTC TTGGATTCTC TCCTGGCCAC GTCCTTCTAC 
AATTACCATG TCGTGGAATT ACTTCAGATG CTAGTGACTG GAGGCATAAG CTCTGAGATG 
GAACACTATT TGGTTAAGGA GAAGCCCTAT AAGACAACTG ACGACTATGA GGCAATCAAG 
TCTGGGAGGA CGCGGTGTAA GCTGGGACTC CTCTCTTTAG ACCAAACCGT TCTATCAGGC 
ATTAATCCAA GAAAAACCTT TGGACAGCTG TTCTGTGGCT CATTGGATAA TTTCGGGATC 
CTATGTGTCG GCTTATACCG TATGATTGAT GAAGAGGAAC CCAGCCAAGA ACACAAAAGG 
TTTGTGATCA CCAGGCCATC CAATGAGTGC CACCTGCTGC CCTCAGATCT CGTGTTTTGT 
GCCATCCCTT TCAACACCAC CTGTGGCAAA TCAGACAGCA GTCCTTCAAT TCAGGCTCAA 
AACAACTCTA CAAACGCGAC GACGCCATTG GCCCAGGGGT CGAATTTCTT CGATTCGCAC 
CATGCCGACG AGTCCCACGA TCTTTACCCA GTCGACGACA CGGGAGAGAG GTGGTCTCAG 
CACCACCACT CCCGAGTCTA TCCTTTGGAC ACGTTAGATG CCAGTGATAT TGTTCAAGAA 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnu1 ( XM_017312582.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168071.1 Mus musculus potassium channel, subfamily U, member 1 (Kcnu1), transcript variant X3, mRNA.

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CloneID	OMu82356
Clone ID Related Accession (Same CDS sequence)	XM_017312582.1
Accession Version	XM_017312582.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily U member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAAA CATTGCTAGA CAGTTTAAAT CAGAAGGAGT TGACGGAAAC GTCATGTACA 
ATCGAAATCC AGGCAGCGTT CATTCTTTCC TCCTTGGCGA CTTTCTTCGG GGGACTCATC 
ATCTTATTCC TTTTCAGAAT AGCCTTGAAA AGCTCAAGAA GTTGGAAATA CGTCAAGGGG 
CCAAGAGGAC TCTTGGAACT ATTCTCATCA CGTAGAATCG AGGCTAATCC TTTGAGGAAA 
CTTTACTTTC ATGGAGTATT TCGTCAGCGC ATCGAAATGC TGCTTTCTGC ACAGACCGTC 
GTGGGGCAAG TGTTGGTGAT CCTTGTCTTT GTACTAAGCA TCGGGTCTCT TGTGATCTAT 
TTCATCAATT CAATGGATCC TGTTCGAAGG TGTTCTTCAT ATGAAGACAA AATTGTCCAT 
GTGGATTTGA GTTTCAACGC TTTCTTTAGC TTCTATTTTG GGTTGAGGTT TTGGGCAGCT 
GAAGACAAGA TCAAGTTCTG GTTGGAGATG AATTCAATTG TAGACATTTT TACCATCCCG 
CCAACCTTTA TTTCTTATTA TTTGAAGAGT AATTGGCTAG GTTTGAGATT TCTAAGAGCT 
CTGCGGTTGC TCGAACTCCC TAAAATCTTA CAGATCCTAC AAGTCATCAA GACCAGCAAT 
TCAGTGAAGC TTTCCAAACT GTTGTCAATA GTTATCAGTA CCTGGTTCAC GGCAGCAGGA 
TTCCTTCACC TGACCTTTCC TTTGCCATCT CTCGTTGGAC AGGTGGAAAA TTCTGGTGAC 
CCCTGGCTCA ACGGAAGAAA CTCACAGACT ATGTCATACT TTGAGTCTAT TTATCTGGTG 
ACAGCAACAA TGTCAACTGT TGGCTTTGGG GACGTGGTGG CCAAGACATC CCTAGGACGG 
ATTTTCATTG TTTTCTTCAC CCTTGGGAGT TTGATACTAT TTGCAAACTA CATTCCAGAA 
ATGGTGGAGC TCTTTTCTAC CAGGAAGAAA TACACCAAGC CCTACGAAGC AGTCAAAGGA 
AAAAAGTTCA TCGTGGTCTG TGGAAACATC ACAGTTGACA GTGTTACTGC TTTCCTGAGG 
AATTTTCTCC ACTGGAAGTC CGGGGAAATC AATATTGAGA TCGTATTCCT TGGAGAGACT 
CTCCCTTGCT TGGAACTGGA GACCTTACTG AAGTGCCACA CATCCTGTAC CAACTTCGTA 
TGCGGCACCG CACTGAAGTT CGAGGATCTG AAGCGAGTTG CAGTGGAGAA CTCGGAGGCG 
TGCCTGATTC TAGCCAACCA TTTCTGTAGT GACTTACATG ACGAAGACAA CTCAAACATT 
ATGAGGGTGC TCTCGATCAA GAACTATTAT CCACAGACCA GAGTCATCAT TCAGATACTT 
CAGTCTCAAA ACAAGGTTTT CCTGTCAAAA ATCCCCAACT GGGACTGGAG TGCTGGAGAC 
AATATCCTCT GCTTTGCAGA GCTAAAGCTC GGATTTATCG CCCAAGGCTG CTTGGTGCCA 
GGGCTGTGCA CCTTTCTCAC GACTCTGTTC ATTGAACAAA ACCAAAAGGT TTTTCCTAAA 
CATCCCTGGC AAAAACATTT CTTGAATGGC TTGAAGAACA AGATTCTGAC ACAGCGCCTC 
TCTAACGACT TCGTGGGGAT GACATTTCCC CAGGTCTCCC GGCTCTGCTT TGTGAAGCTA 
AATCTCATGC TGATCGCCAT CCAACACAAG CCCTTCTTTC ACAGTTGTTG CACTCTGATA 
CTAAACCCAT CATCCCAAGT GAGGCTGAAT AAGGACACCT TAGGGTTCTT CATTGCGGAC 
TCCTCCAAAG CCGTCAAAAG GGCTTTCTTT TACTGTTCCA ACTGTCACAG CGATGTGTGC 
AATCCTGAGC TAATTGGAAA GTGTAACTGT AAAATCAAGA GCCGACAACA ACTCATAGCA 
CCGACCATCA TGGTGATGAA AAGCAGCTTG ACCGATTTCA CCACTTCTTC ACACATCCAC 
GCTTCTATGT CAACAGAAAT TCACACTTGT TTTTCAAGAG AACAGCCTAG TTTGATCACC 
ATTACAACCA ACAGACCAAC GACAAACGAC ACAGTGGATG ATACCGACAT GCTGGACAGC 
AGTGGCATGT TTCACTGGTG CAGAGCAATG CCCTTGGACA AGGTGGTTCT GAAACGAAGT 
GAGAAGGCAA AACACGAGTT TCAGAACCAC ATTGTAGTAT GCGTGTTTGG AGATGCCCAA 
TGTACCCTGG TGGGGCTTCG GAATTTCGTG ATGCCCCTGA GAGCCAGCAA CTACACCCGG 
CAGGAGCTGA AGGACATTGT TTTTATTGGG TCTCTGGAGT ACTTCCAGAG AGAATGGCGA 
TTTCTCCGAA ACTTTCCCAA GATACACATT ATGCCTGGAT CTGCACTCTA CATGGGAGAT 
CTGATTGCAG TCAATGTAGA GCAGTGCTCT ATGTGCGTCA TCTTAGCCAC ACCCTACAAG 
GCACTGAGCA GCCAGATTCT GGTGGACACA GAGGCCATCA TGGCCACCCT CAACATCCAG 
TCCCTGCGGA TCACCAGTCC TACTCCAGGG TCTTCAAAGT CAGAAGTAAA GCCATCATCT 
GCCTTTGATA GTAAAGAAAG GAAGCAAAGA TACAAACAGA TCCCCATTCT CACTGAACTG 
AAGAATCCCT CCAACATCCA CTTTATTGAG CAGATGGGCG GACTGGATGG AATGCTCAAA 
GGGACTAGCT TGCATCTCAG CACTTCTTTC TCCACCGGTG CTGTCTTTTC AGACACCTTC 
TTGGATTCTC TCCTGGCCAC GCCAAGAAAA ACCTTTGGAC AGCTGTTCTG TGGCTCATTG 
GATAATTTCG GGATCCTATG TGTCGGCTTA TACCGTATGA TTGATGAAGA GGAACCCAGC 
CAAGAACACA AAAGGTTTGT GATCACCAGG CCATCCAATG AGTGCCACCT GCTGCCCTCA 
GATCTCGTGT TTTGTGCCAT CCCTTTCAAC ACCACCTGTG GCAAATCAGA CAGCAGTCCT 
TCAATTCAGG CTCAAAACAA CTCTACAAAC GCGACGACGC CATTGGCCCA GGGGTCGAAT 
TTCTTCGATT CGCACCATGC CGACGAGTCC CACGATCTTT ACCCAGTCGA CGACACGGGA 
GAGAGGTGGT CTCAGCACCA CCACTCCCGA GTCTATCCTT TGGACACGTT AGATGCCAGT 
GATATTGTTC AAGAAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168071.1
CDS	33..3233
Translation	MSQTLLDSLNQKELTETSCTIEIQAAFILSSLATFFGGLIILFLFRIALKSSRSWKYVKGPRGLLELFSSRRIEANPLRKLYFHGVFRQRIEMLLSAQTVVGQVLVILVFVLSIGSLVIYFINSMDPVRRCSSYEDKIVHVDLSFNAFFSFYFGLRFWAAEDKIKFWLEMNSIVDIFTIPPTFISYYLKSNWLGLRFLRALRLLELPKILQILQVIKTSNSVKLSKLLSIVISTWFTAAGFLHLTFPLPSLVGQVENSGDPWLNGRNSQTMSYFESIYLVTATMSTVGFGDVVAKTSLGRIFIVFFTLGSLILFANYIPEMVELFSTRKKYTKPYEAVKGKKFIVVCGNITVDSVTAFLRNFLHWKSGEINIEIVFLGETLPCLELETLLKCHTSCTNFVCGTALKFEDLKRVAVENSEACLILANHFCSDLHDEDNSNIMRVLSIKNYYPQTRVIIQILQSQNKVFLSKIPNWDWSAGDNILCFAELKLGFIAQGCLVPGLCTFLTTLFIEQNQKVFPKHPWQKHFLNGLKNKILTQRLSNDFVGMTFPQVSRLCFVKLNLMLIAIQHKPFFHSCCTLILNPSSQVRLNKDTLGFFIADSSKAVKRAFFYCSNCHSDVCNPELIGKCNCKIKSRQQLIAPTIMVMKSSLTDFTTSSHIHASMSTEIHTCFSREQPSLITITTNRPTTNDTVDDTDMLDSSGMFHWCRAMPLDKVVLKRSEKAKHEFQNHIVVCVFGDAQCTLVGLRNFVMPLRASNYTRQELKDIVFIGSLEYFQREWRFLRNFPKIHIMPGSALYMGDLIAVNVEQCSMCVILATPYKALSSQILVDTEAIMATLNIQSLRITSPTPGSSKSEVKPSSAFDSKERKQRYKQIPILTELKNPSNIHFIEQMGGLDGMLKGTSLHLSTSFSTGAVFSDTFLDSLLATPRKTFGQLFCGSLDNFGILCVGLYRMIDEEEPSQEHKRFVITRPSNECHLLPSDLVFCAIPFNTTCGKSDSSPSIQAQNNSTNATTPLAQGSNFFDSHHADESHDLYPVDDTGERWSQHHHSRVYPLDTLDASDIVQEK

Target ORF information:

RefSeq Version	XM_017312582.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily U, member 1 (Kcnu1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312582.1

ORF Insert Sequence:

ATGTCTCAAA CATTGCTAGA CAGTTTAAAT CAGAAGGAGT TGACGGAAAC GTCATGTACA 
ATCGAAATCC AGGCAGCGTT CATTCTTTCC TCCTTGGCGA CTTTCTTCGG GGGACTCATC 
ATCTTATTCC TTTTCAGAAT AGCCTTGAAA AGCTCAAGAA GTTGGAAATA CGTCAAGGGG 
CCAAGAGGAC TCTTGGAACT ATTCTCATCA CGTAGAATCG AGGCTAATCC TTTGAGGAAA 
CTTTACTTTC ATGGAGTATT TCGTCAGCGC ATCGAAATGC TGCTTTCTGC ACAGACCGTC 
GTGGGGCAAG TGTTGGTGAT CCTTGTCTTT GTACTAAGCA TCGGGTCTCT TGTGATCTAT 
TTCATCAATT CAATGGATCC TGTTCGAAGG TGTTCTTCAT ATGAAGACAA AATTGTCCAT 
GTGGATTTGA GTTTCAACGC TTTCTTTAGC TTCTATTTTG GGTTGAGGTT TTGGGCAGCT 
GAAGACAAGA TCAAGTTCTG GTTGGAGATG AATTCAATTG TAGACATTTT TACCATCCCG 
CCAACCTTTA TTTCTTATTA TTTGAAGAGT AATTGGCTAG GTTTGAGATT TCTAAGAGCT 
CTGCGGTTGC TCGAACTCCC TAAAATCTTA CAGATCCTAC AAGTCATCAA GACCAGCAAT 
TCAGTGAAGC TTTCCAAACT GTTGTCAATA GTTATCAGTA CCTGGTTCAC GGCAGCAGGA 
TTCCTTCACC TGACCTTTCC TTTGCCATCT CTCGTTGGAC AGGTGGAAAA TTCTGGTGAC 
CCCTGGCTCA ACGGAAGAAA CTCACAGACT ATGTCATACT TTGAGTCTAT TTATCTGGTG 
ACAGCAACAA TGTCAACTGT TGGCTTTGGG GACGTGGTGG CCAAGACATC CCTAGGACGG 
ATTTTCATTG TTTTCTTCAC CCTTGGGAGT TTGATACTAT TTGCAAACTA CATTCCAGAA 
ATGGTGGAGC TCTTTTCTAC CAGGAAGAAA TACACCAAGC CCTACGAAGC AGTCAAAGGA 
AAAAAGTTCA TCGTGGTCTG TGGAAACATC ACAGTTGACA GTGTTACTGC TTTCCTGAGG 
AATTTTCTCC ACTGGAAGTC CGGGGAAATC AATATTGAGA TCGTATTCCT TGGAGAGACT 
CTCCCTTGCT TGGAACTGGA GACCTTACTG AAGTGCCACA CATCCTGTAC CAACTTCGTA 
TGCGGCACCG CACTGAAGTT CGAGGATCTG AAGCGAGTTG CAGTGGAGAA CTCGGAGGCG 
TGCCTGATTC TAGCCAACCA TTTCTGTAGT GACTTACATG ACGAAGACAA CTCAAACATT 
ATGAGGGTGC TCTCGATCAA GAACTATTAT CCACAGACCA GAGTCATCAT TCAGATACTT 
CAGTCTCAAA ACAAGGTTTT CCTGTCAAAA ATCCCCAACT GGGACTGGAG TGCTGGAGAC 
AATATCCTCT GCTTTGCAGA GCTAAAGCTC GGATTTATCG CCCAAGGCTG CTTGGTGCCA 
GGGCTGTGCA CCTTTCTCAC GACTCTGTTC ATTGAACAAA ACCAAAAGGT TTTTCCTAAA 
CATCCCTGGC AAAAACATTT CTTGAATGGC TTGAAGAACA AGATTCTGAC ACAGCGCCTC 
TCTAACGACT TCGTGGGGAT GACATTTCCC CAGGTCTCCC GGCTCTGCTT TGTGAAGCTA 
AATCTCATGC TGATCGCCAT CCAACACAAG CCCTTCTTTC ACAGTTGTTG CACTCTGATA 
CTAAACCCAT CATCCCAAGT GAGGCTGAAT AAGGACACCT TAGGGTTCTT CATTGCGGAC 
TCCTCCAAAG CCGTCAAAAG GGCTTTCTTT TACTGTTCCA ACTGTCACAG CGATGTGTGC 
AATCCTGAGC TAATTGGAAA GTGTAACTGT AAAATCAAGA GCCGACAACA ACTCATAGCA 
CCGACCATCA TGGTGATGAA AAGCAGCTTG ACCGATTTCA CCACTTCTTC ACACATCCAC 
GCTTCTATGT CAACAGAAAT TCACACTTGT TTTTCAAGAG AACAGCCTAG TTTGATCACC 
ATTACAACCA ACAGACCAAC GACAAACGAC ACAGTGGATG ATACCGACAT GCTGGACAGC 
AGTGGCATGT TTCACTGGTG CAGAGCAATG CCCTTGGACA AGGTGGTTCT GAAACGAAGT 
GAGAAGGCAA AACACGAGTT TCAGAACCAC ATTGTAGTAT GCGTGTTTGG AGATGCCCAA 
TGTACCCTGG TGGGGCTTCG GAATTTCGTG ATGCCCCTGA GAGCCAGCAA CTACACCCGG 
CAGGAGCTGA AGGACATTGT TTTTATTGGG TCTCTGGAGT ACTTCCAGAG AGAATGGCGA 
TTTCTCCGAA ACTTTCCCAA GATACACATT ATGCCTGGAT CTGCACTCTA CATGGGAGAT 
CTGATTGCAG TCAATGTAGA GCAGTGCTCT ATGTGCGTCA TCTTAGCCAC ACCCTACAAG 
GCACTGAGCA GCCAGATTCT GGTGGACACA GAGGCCATCA TGGCCACCCT CAACATCCAG 
TCCCTGCGGA TCACCAGTCC TACTCCAGGG TCTTCAAAGT CAGAAGTAAA GCCATCATCT 
GCCTTTGATA GTAAAGAAAG GAAGCAAAGA TACAAACAGA TCCCCATTCT CACTGAACTG 
AAGAATCCCT CCAACATCCA CTTTATTGAG CAGATGGGCG GACTGGATGG AATGCTCAAA 
GGGACTAGCT TGCATCTCAG CACTTCTTTC TCCACCGGTG CTGTCTTTTC AGACACCTTC 
TTGGATTCTC TCCTGGCCAC GCCAAGAAAA ACCTTTGGAC AGCTGTTCTG TGGCTCATTG 
GATAATTTCG GGATCCTATG TGTCGGCTTA TACCGTATGA TTGATGAAGA GGAACCCAGC 
CAAGAACACA AAAGGTTTGT GATCACCAGG CCATCCAATG AGTGCCACCT GCTGCCCTCA 
GATCTCGTGT TTTGTGCCAT CCCTTTCAAC ACCACCTGTG GCAAATCAGA CAGCAGTCCT 
TCAATTCAGG CTCAAAACAA CTCTACAAAC GCGACGACGC CATTGGCCCA GGGGTCGAAT 
TTCTTCGATT CGCACCATGC CGACGAGTCC CACGATCTTT ACCCAGTCGA CGACACGGGA 
GAGAGGTGGT CTCAGCACCA CCACTCCCGA GTCTATCCTT TGGACACGTT AGATGCCAGT 
GATATTGTTC AAGAAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnu1 ( NM_008432.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032458.3 Mus musculus potassium channel, subfamily U, member 1 (Kcnu1), mRNA.

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CloneID	OMu19323
Clone ID Related Accession (Same CDS sequence)	NM_008432.3
Accession Version	NM_008432.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily U member 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ042506.1, AF039213.2 and BQ175543.1. On Dec 28, 2006 this sequence version replaced NM_008432.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF039213.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGTCTCAAA CATTGCTAGA CAGTTTAAAT CAGAAGGAGT TGACGGAAAC GTCATGTACA 
ATCGAAATCC AGGCAGCGTT CATTCTTTCC TCCTTGGCGA CTTTCTTCGG GGGACTCATC 
ATCTTATTCC TTTTCAGAAT AGCCTTGAAA AGCTCAAGAA GTTGGAAATA CGTCAAGGGG 
CCAAGAGGAC TCTTGGAACT ATTCTCATCA CGTAGAATCG AGGCTAATCC TTTGAGGAAA 
CTTTACTTTC ATGGAGTATT TCGTCAGCGC ATCGAAATGC TGCTTTCTGC ACAGACCGTC 
GTGGGGCAAG TGTTGGTGAT CCTTGTCTTT GTACTAAGCA TCGGGTCTCT TGTGATCTAT 
TTCATCAATT CAATGGATCC TGTTCGAAGG TGTTCTTCAT ATGAAGACAA AATTGTCCAT 
GTGGATTTGA GTTTCAACGC TTTCTTTAGC TTCTATTTTG GGTTGAGGTT TTGGGCAGCT 
GAAGACAAGA TCAAGTTCTG GTTGGAGATG AATTCAATTG TAGACATTTT TACCATCCCG 
CCAACCTTTA TTTCTTATTA TTTGAAGAGT AATTGGCTAG GTTTGAGATT TCTAAGAGCT 
CTGCGGTTGC TCGAACTCCC TAAAATCTTA CAGATCCTAC AAGTCATCAA GACCAGCAAT 
TCAGTGAAGC TTTCCAAACT GTTGTCAATA GTTATCAGTA CCTGGTTCAC GGCAGCAGGA 
TTCCTTCACC TGGTGGAAAA TTCTGGTGAC CCCTGGCTCA ACGGAAGAAA CTCACAGACT 
ATGTCATACT TTGAGTCTAT TTATCTGGTG ACAGCAACAA TGTCAACTGT TGGCTTTGGG 
GACGTGGTGG CCAAGACATC CCTAGGACGG ATTTTCATTG TTTTCTTCAC CCTTGGGAGT 
TTGATACTAT TTGCAAACTA CATTCCAGAA ATGGTGGAGC TCTTTTCTAC CAGGAAGAAA 
TACACCAAGC CCTACGAAGC AGTCAAAGGA AAAAAGTTCA TCGTGGTCTG TGGAAACATC 
ACAGTTGACA GTGTTACTGC TTTCCTGAGG AATTTTCTCC ACTGGAAGTC CGGGGAAATC 
AATATTGAGA TCGTATTCCT TGGAGAGACT CTCCCTTGCT TGGAACTGGA GACCTTACTG 
AAGTGCCACA CATCCTGTAC CAACTTCGTA TGCGGCACCG CACTGAAGTT CGAGGATCTG 
AAGCGAGTTG CAGTGGAGAA CTCGGAGGCG TGCCTGATTC TAGCCAACCA TTTCTGTAGT 
GACTTACATG ACGAAGACAA CTCAAACATT ATGAGGGTGC TCTCGATCAA GAACTATTAT 
CCACAGACCA GAGTCATCAT TCAGATACTT CAGTCTCAAA ACAAGGTTTT CCTGTCAAAA 
ATCCCCAACT GGGACTGGAG TGCTGGAGAC AATATCCTCT GCTTTGCAGA GCTAAAGCTC 
GGATTTATCG CCCAAGGCTG CTTGGTGCCA GGGCTGTGCA CCTTTCTCAC GACTCTGTTC 
ATTGAACAAA ACCAAAAGGT TTTTCCTAAA CATCCCTGGC AAAAACATTT CTTGAATGGC 
TTGAAGAACA AGATTCTGAC ACAGCGCCTC TCTAACGACT TCGTGGGGAT GACATTTCCC 
CAGGTCTCCC GGCTCTGCTT TGTGAAGCTA AATCTCATGC TGATCGCCAT CCAACACAAG 
CCCTTCTTTC ACAGTTGTTG CACTCTGATA CTAAACCCAT CATCCCAAGT GAGGCTGAAT 
AAGGACACCT TAGGGTTCTT CATTGCGGAC TCCTCCAAAG CCGTCAAAAG GGCTTTCTTT 
TACTGTTCCA ACTGTCACAG CGATGTGTGC AATCCTGAGC TAATTGGAAA GTGTAACTGT 
AAAATCAAGA GCCGACAACA ACTCATAGCA CCGACCATCA TGGTGATGAA AAGCAGCTTG 
ACCGATTTCA CCACTTCTTC ACACATCCAC GCTTCTATGT CAACAGAAAT TCACACTTGT 
TTTTCAAGAG AACAGCCTAG TTTGATCACC ATTACAACCA ACAGACCAAC GACAAACGAC 
ACAGTGGATG ATACCGACAT GCTGGACAGC AGTGGCATGT TTCACTGGTG CAGAGCAATG 
CCCTTGGACA AGGTGGTTCT GAAACGAAGT GAGAAGGCAA AACACGAGTT TCAGAACCAC 
ATTGTAGTAT GCGTGTTTGG AGATGCCCAA TGTACCCTGG TGGGGCTTCG GAATTTCGTG 
ATGCCCCTGA GAGCCAGCAA CTACACCCGG CAGGAGCTGA AGGACATTGT TTTTATTGGG 
TCTCTGGAGT ACTTCCAGAG AGAATGGCGA TTTCTCCGAA ACTTTCCCAA GATACACATT 
ATGCCTGGAT CTGCACTCTA CATGGGAGAT CTGATTGCAG TCAATGTAGA GCAGTGCTCT 
ATGTGCGTCA TCTTAGCCAC ACCCTACAAG GCACTGAGCA GCCAGATTCT GGTGGACACA 
GAGGCCATCA TGGCCACCCT CAACATCCAG TCCCTGCGGA TCACCAGTCC TACTCCAGGG 
TCTTCAAAGT CAGAAGTAAA GCCATCATCT GCCTTTGATA GTAAAGAAAG GAAGCAAAGA 
TACAAACAGA TCCCCATTCT CACTGAACTG AAGAATCCCT CCAACATCCA CTTTATTGAG 
CAGATGGGCG GACTGGATGG AATGCTCAAA GGGACTAGCT TGCATCTCAG CACTTCTTTC 
TCCACCGGTG CTGTCTTTTC AGACACCTTC TTGGATTCTC TCCTGGCCAC GTCCTTCTAC 
AATTACCATG TCGTGGAATT ACTTCAGATG CTAGTGACTG GAGGCATAAG CTCTGAGATG 
GAACACTATT TGGTTAAGGA GAAGCCCTAT AAGACAACTG ACGACTATGA GGCAATCAAG 
TCTGGGAGGA CGCGGTGTAA GCTGGGACTC CTCTCTTTAG ACCAAACCGT TCTATCAGGC 
ATTAATCCAA GAAAAACCTT TGGACAGCTG TTCTGTGGCT CATTGGATAA TTTCGGGATC 
CTATGTGTCG GCTTATACCG TATGATTGAT GAAGAGGAAC CCAGCCAAGA ACACAAAAGG 
TTTGTGATCA CCAGGCCATC CAATGAGTGC CACCTGCTGC CCTCAGATCT CGTGTTTTGT 
GCCATCCCTT TCAACACCAC CTGTGGCAAA TCAGACAGCA GTCCTTCAAT TCAGGCTCAA 
AACAACTCTA CAAACGCGAC GACGCCATTG GCCCAGGGGT CGAATTTCTT CGATTCGCAC 
CATGCCGACG AGTCCCACGA TCTTTACCCA GTCGACGACA CGGGAGAGAG GTGGTCTCAG 
CACCACCACT CCCGAGTCTA TCCTTTGGAC ACGTTAGATG CCAGTGATAT TGTTCAAGAA 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032458.3
CDS	34..3399
Translation	MSQTLLDSLNQKELTETSCTIEIQAAFILSSLATFFGGLIILFLFRIALKSSRSWKYVKGPRGLLELFSSRRIEANPLRKLYFHGVFRQRIEMLLSAQTVVGQVLVILVFVLSIGSLVIYFINSMDPVRRCSSYEDKIVHVDLSFNAFFSFYFGLRFWAAEDKIKFWLEMNSIVDIFTIPPTFISYYLKSNWLGLRFLRALRLLELPKILQILQVIKTSNSVKLSKLLSIVISTWFTAAGFLHLVENSGDPWLNGRNSQTMSYFESIYLVTATMSTVGFGDVVAKTSLGRIFIVFFTLGSLILFANYIPEMVELFSTRKKYTKPYEAVKGKKFIVVCGNITVDSVTAFLRNFLHWKSGEINIEIVFLGETLPCLELETLLKCHTSCTNFVCGTALKFEDLKRVAVENSEACLILANHFCSDLHDEDNSNIMRVLSIKNYYPQTRVIIQILQSQNKVFLSKIPNWDWSAGDNILCFAELKLGFIAQGCLVPGLCTFLTTLFIEQNQKVFPKHPWQKHFLNGLKNKILTQRLSNDFVGMTFPQVSRLCFVKLNLMLIAIQHKPFFHSCCTLILNPSSQVRLNKDTLGFFIADSSKAVKRAFFYCSNCHSDVCNPELIGKCNCKIKSRQQLIAPTIMVMKSSLTDFTTSSHIHASMSTEIHTCFSREQPSLITITTNRPTTNDTVDDTDMLDSSGMFHWCRAMPLDKVVLKRSEKAKHEFQNHIVVCVFGDAQCTLVGLRNFVMPLRASNYTRQELKDIVFIGSLEYFQREWRFLRNFPKIHIMPGSALYMGDLIAVNVEQCSMCVILATPYKALSSQILVDTEAIMATLNIQSLRITSPTPGSSKSEVKPSSAFDSKERKQRYKQIPILTELKNPSNIHFIEQMGGLDGMLKGTSLHLSTSFSTGAVFSDTFLDSLLATSFYNYHVVELLQMLVTGGISSEMEHYLVKEKPYKTTDDYEAIKSGRTRCKLGLLSLDQTVLSGINPRKTFGQLFCGSLDNFGILCVGLYRMIDEEEPSQEHKRFVITRPSNECHLLPSDLVFCAIPFNTTCGKSDSSPSIQAQNNSTNATTPLAQGSNFFDSHHADESHDLYPVDDTGERWSQHHHSRVYPLDTLDASDIVQEK

Target ORF information:

RefSeq Version	NM_008432.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily U, member 1 (Kcnu1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008432.3

ORF Insert Sequence:

ATGTCTCAAA CATTGCTAGA CAGTTTAAAT CAGAAGGAGT TGACGGAAAC GTCATGTACA 
ATCGAAATCC AGGCAGCGTT CATTCTTTCC TCCTTGGCGA CTTTCTTCGG GGGACTCATC 
ATCTTATTCC TTTTCAGAAT AGCCTTGAAA AGCTCAAGAA GTTGGAAATA CGTCAAGGGG 
CCAAGAGGAC TCTTGGAACT ATTCTCATCA CGTAGAATCG AGGCTAATCC TTTGAGGAAA 
CTTTACTTTC ATGGAGTATT TCGTCAGCGC ATCGAAATGC TGCTTTCTGC ACAGACCGTC 
GTGGGGCAAG TGTTGGTGAT CCTTGTCTTT GTACTAAGCA TCGGGTCTCT TGTGATCTAT 
TTCATCAATT CAATGGATCC TGTTCGAAGG TGTTCTTCAT ATGAAGACAA AATTGTCCAT 
GTGGATTTGA GTTTCAACGC TTTCTTTAGC TTCTATTTTG GGTTGAGGTT TTGGGCAGCT 
GAAGACAAGA TCAAGTTCTG GTTGGAGATG AATTCAATTG TAGACATTTT TACCATCCCG 
CCAACCTTTA TTTCTTATTA TTTGAAGAGT AATTGGCTAG GTTTGAGATT TCTAAGAGCT 
CTGCGGTTGC TCGAACTCCC TAAAATCTTA CAGATCCTAC AAGTCATCAA GACCAGCAAT 
TCAGTGAAGC TTTCCAAACT GTTGTCAATA GTTATCAGTA CCTGGTTCAC GGCAGCAGGA 
TTCCTTCACC TGGTGGAAAA TTCTGGTGAC CCCTGGCTCA ACGGAAGAAA CTCACAGACT 
ATGTCATACT TTGAGTCTAT TTATCTGGTG ACAGCAACAA TGTCAACTGT TGGCTTTGGG 
GACGTGGTGG CCAAGACATC CCTAGGACGG ATTTTCATTG TTTTCTTCAC CCTTGGGAGT 
TTGATACTAT TTGCAAACTA CATTCCAGAA ATGGTGGAGC TCTTTTCTAC CAGGAAGAAA 
TACACCAAGC CCTACGAAGC AGTCAAAGGA AAAAAGTTCA TCGTGGTCTG TGGAAACATC 
ACAGTTGACA GTGTTACTGC TTTCCTGAGG AATTTTCTCC ACTGGAAGTC CGGGGAAATC 
AATATTGAGA TCGTATTCCT TGGAGAGACT CTCCCTTGCT TGGAACTGGA GACCTTACTG 
AAGTGCCACA CATCCTGTAC CAACTTCGTA TGCGGCACCG CACTGAAGTT CGAGGATCTG 
AAGCGAGTTG CAGTGGAGAA CTCGGAGGCG TGCCTGATTC TAGCCAACCA TTTCTGTAGT 
GACTTACATG ACGAAGACAA CTCAAACATT ATGAGGGTGC TCTCGATCAA GAACTATTAT 
CCACAGACCA GAGTCATCAT TCAGATACTT CAGTCTCAAA ACAAGGTTTT CCTGTCAAAA 
ATCCCCAACT GGGACTGGAG TGCTGGAGAC AATATCCTCT GCTTTGCAGA GCTAAAGCTC 
GGATTTATCG CCCAAGGCTG CTTGGTGCCA GGGCTGTGCA CCTTTCTCAC GACTCTGTTC 
ATTGAACAAA ACCAAAAGGT TTTTCCTAAA CATCCCTGGC AAAAACATTT CTTGAATGGC 
TTGAAGAACA AGATTCTGAC ACAGCGCCTC TCTAACGACT TCGTGGGGAT GACATTTCCC 
CAGGTCTCCC GGCTCTGCTT TGTGAAGCTA AATCTCATGC TGATCGCCAT CCAACACAAG 
CCCTTCTTTC ACAGTTGTTG CACTCTGATA CTAAACCCAT CATCCCAAGT GAGGCTGAAT 
AAGGACACCT TAGGGTTCTT CATTGCGGAC TCCTCCAAAG CCGTCAAAAG GGCTTTCTTT 
TACTGTTCCA ACTGTCACAG CGATGTGTGC AATCCTGAGC TAATTGGAAA GTGTAACTGT 
AAAATCAAGA GCCGACAACA ACTCATAGCA CCGACCATCA TGGTGATGAA AAGCAGCTTG 
ACCGATTTCA CCACTTCTTC ACACATCCAC GCTTCTATGT CAACAGAAAT TCACACTTGT 
TTTTCAAGAG AACAGCCTAG TTTGATCACC ATTACAACCA ACAGACCAAC GACAAACGAC 
ACAGTGGATG ATACCGACAT GCTGGACAGC AGTGGCATGT TTCACTGGTG CAGAGCAATG 
CCCTTGGACA AGGTGGTTCT GAAACGAAGT GAGAAGGCAA AACACGAGTT TCAGAACCAC 
ATTGTAGTAT GCGTGTTTGG AGATGCCCAA TGTACCCTGG TGGGGCTTCG GAATTTCGTG 
ATGCCCCTGA GAGCCAGCAA CTACACCCGG CAGGAGCTGA AGGACATTGT TTTTATTGGG 
TCTCTGGAGT ACTTCCAGAG AGAATGGCGA TTTCTCCGAA ACTTTCCCAA GATACACATT 
ATGCCTGGAT CTGCACTCTA CATGGGAGAT CTGATTGCAG TCAATGTAGA GCAGTGCTCT 
ATGTGCGTCA TCTTAGCCAC ACCCTACAAG GCACTGAGCA GCCAGATTCT GGTGGACACA 
GAGGCCATCA TGGCCACCCT CAACATCCAG TCCCTGCGGA TCACCAGTCC TACTCCAGGG 
TCTTCAAAGT CAGAAGTAAA GCCATCATCT GCCTTTGATA GTAAAGAAAG GAAGCAAAGA 
TACAAACAGA TCCCCATTCT CACTGAACTG AAGAATCCCT CCAACATCCA CTTTATTGAG 
CAGATGGGCG GACTGGATGG AATGCTCAAA GGGACTAGCT TGCATCTCAG CACTTCTTTC 
TCCACCGGTG CTGTCTTTTC AGACACCTTC TTGGATTCTC TCCTGGCCAC GTCCTTCTAC 
AATTACCATG TCGTGGAATT ACTTCAGATG CTAGTGACTG GAGGCATAAG CTCTGAGATG 
GAACACTATT TGGTTAAGGA GAAGCCCTAT AAGACAACTG ACGACTATGA GGCAATCAAG 
TCTGGGAGGA CGCGGTGTAA GCTGGGACTC CTCTCTTTAG ACCAAACCGT TCTATCAGGC 
ATTAATCCAA GAAAAACCTT TGGACAGCTG TTCTGTGGCT CATTGGATAA TTTCGGGATC 
CTATGTGTCG GCTTATACCG TATGATTGAT GAAGAGGAAC CCAGCCAAGA ACACAAAAGG 
TTTGTGATCA CCAGGCCATC CAATGAGTGC CACCTGCTGC CCTCAGATCT CGTGTTTTGT 
GCCATCCCTT TCAACACCAC CTGTGGCAAA TCAGACAGCA GTCCTTCAAT TCAGGCTCAA 
AACAACTCTA CAAACGCGAC GACGCCATTG GCCCAGGGGT CGAATTTCTT CGATTCGCAC 
CATGCCGACG AGTCCCACGA TCTTTACCCA GTCGACGACA CGGGAGAGAG GTGGTCTCAG 
CACCACCACT CCCGAGTCTA TCCTTTGGAC ACGTTAGATG CCAGTGATAT TGTTCAAGAA 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnu1 ( XM_006509024.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509087.1 Mus musculus potassium channel, subfamily U, member 1 (Kcnu1), transcript variant X1, mRNA.

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CloneID	OMu40570
Clone ID Related Accession (Same CDS sequence)	XM_006509024.2
Accession Version	XM_006509024.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily U member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006509024.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAAA CATTGCTAGA CAGTTTAAAT CAGAAGGAGT TGACGGAAAC GTCATGTACA 
ATCGAAATCC AGGCAGCGTT CATTCTTTCC TCCTTGGCGA CTTTCTTCGG GGGACTCATC 
ATCTTATTCC TTTTCAGAAT AGCCTTGAAA AGCTCAAGAA GTTGGAAATA CGTCAAGGGG 
CCAAGAGGAC TCTTGGAACT ATTCTCATCA CGTAGAATCG AGGCTAATCC TTTGAGGAAA 
CTTTACTTTC ATGGAGTATT TCGTCAGCGC ATCGAAATGC TGCTTTCTGC ACAGACCGTC 
GTGGGGCAAG TGTTGGTGAT CCTTGTCTTT GTACTAAGCA TCGGGTCTCT TGTGATCTAT 
TTCATCAATT CAATGGATCC TGTTCGAAGG TGTTCTTCAT ATGAAGACAA AATTGTCCAT 
GTGGATTTGA GTTTCAACGC TTTCTTTAGC TTCTATTTTG GGTTGAGGTT TTGGGCAGCT 
GAAGACAAGA TCAAGTTCTG GTTGGAGATG AATTCAATTG TAGACATTTT TACCATCCCG 
CCAACCTTTA TTTCTTATTA TTTGAAGAGT AATTGGCTAG GTTTGAGATT TCTAAGAGCT 
CTGCGGTTGC TCGAACTCCC TAAAATCTTA CAGATCCTAC AAGTCATCAA GACCAGCAAT 
TCAGTGAAGC TTTCCAAACT GTTGTCAATA GTTATCAGTA CCTGGTTCAC GGCAGCAGGA 
TTCCTTCACC TGACCTTTCC TTTGCCATCT CTCGTTGGAC AGGTGGAAAA TTCTGGTGAC 
CCCTGGCTCA ACGGAAGAAA CTCACAGACT ATGTCATACT TTGAGTCTAT TTATCTGGTG 
ACAGCAACAA TGTCAACTGT TGGCTTTGGG GACGTGGTGG CCAAGACATC CCTAGGACGG 
ATTTTCATTG TTTTCTTCAC CCTTGGGAGT TTGATACTAT TTGCAAACTA CATTCCAGAA 
ATGGTGGAGC TCTTTTCTAC CAGGAAGAAA TACACCAAGC CCTACGAAGC AGTCAAAGGA 
AAAAAGTTCA TCGTGGTCTG TGGAAACATC ACAGTTGACA GTGTTACTGC TTTCCTGAGG 
AATTTTCTCC ACTGGAAGTC CGGGGAAATC AATATTGAGA TCGTATTCCT TGGAGAGACT 
CTCCCTTGCT TGGAACTGGA GACCTTACTG AAGTGCCACA CATCCTGTAC CAACTTCGTA 
TGCGGCACCG CACTGAAGTT CGAGGATCTG AAGCGAGTTG CAGTGGAGAA CTCGGAGGCG 
TGCCTGATTC TAGCCAACCA TTTCTGTAGT GACTTACATG ACGAAGACAA CTCAAACATT 
ATGAGGGTGC TCTCGATCAA GAACTATTAT CCACAGACCA GAGTCATCAT TCAGATACTT 
CAGTCTCAAA ACAAGGTTTT CCTGTCAAAA ATCCCCAACT GGGACTGGAG TGCTGGAGAC 
AATATCCTCT GCTTTGCAGA GCTAAAGCTC GGATTTATCG CCCAAGGCTG CTTGGTGCCA 
GGGCTGTGCA CCTTTCTCAC GACTCTGTTC ATTGAACAAA ACCAAAAGGT TTTTCCTAAA 
CATCCCTGGC AAAAACATTT CTTGAATGGC TTGAAGAACA AGATTCTGAC ACAGCGCCTC 
TCTAACGACT TCGTGGGGAT GACATTTCCC CAGGTCTCCC GGCTCTGCTT TGTGAAGCTA 
AATCTCATGC TGATCGCCAT CCAACACAAG CCCTTCTTTC ACAGTTGTTG CACTCTGATA 
CTAAACCCAT CATCCCAAGT GAGGCTGAAT AAGGACACCT TAGGGTTCTT CATTGCGGAC 
TCCTCCAAAG CCGTCAAAAG GGCTTTCTTT TACTGTTCCA ACTGTCACAG CGATGTGTGC 
AATCCTGAGC TAATTGGAAA GTGTAACTGT AAAATCAAGA GCCGACAACA ACTCATAGCA 
CCGACCATCA TGGTGATGAA AAGCAGCTTG ACCGATTTCA CCACTTCTTC ACACATCCAC 
GCTTCTATGT CAACAGAAAT TCACACTTGT TTTTCAAGAG AACAGCCTAG TTTGATCACC 
ATTACAACCA ACAGACCAAC GACAAACGAC ACAGTGGATG ATACCGACAT GCTGGACAGC 
AGTGGCATGT TTCACTGGTG CAGAGCAATG CCCTTGGACA AGGTGGTTCT GAAACGAAGT 
GAGAAGGCAA AACACGAGTT TCAGAACCAC ATTGTAGTAT GCGTGTTTGG AGATGCCCAA 
TGTACCCTGG TGGGGCTTCG GAATTTCGTG ATGCCCCTGA GAGCCAGCAA CTACACCCGG 
CAGGAGCTGA AGGACATTGT TTTTATTGGG TCTCTGGAGT ACTTCCAGAG AGAATGGCGA 
TTTCTCCGAA ACTTTCCCAA GATACACATT ATGCCTGGAT CTGCACTCTA CATGGGAGAT 
CTGATTGCAG TCAATGTAGA GCAGTGCTCT ATGTGCGTCA TCTTAGCCAC ACCCTACAAG 
GCACTGAGCA GCCAGATTCT GGTGGACACA GAGGCCATCA TGGCCACCCT CAACATCCAG 
TCCCTGCGGA TCACCAGTCC TACTCCAGGG TCTTCAAAGT CAGAAGTAAA GCCATCATCT 
GCCTTTGATA GTAAAGAAAG GAAGCAAAGA TACAAACAGA TCCCCATTCT CACTGAACTG 
AAGAATCCCT CCAACATCCA CTTTATTGAG CAGATGGGCG GACTGGATGG AATGCTCAAA 
GGGACTAGCT TGCATCTCAG CACTTCTTTC TCCACCGGTG CTGTCTTTTC AGACACCTTC 
TTGGATTCTC TCCTGGCCAC GTCCTTCTAC AATTACCATG TCGTGGAATT ACTTCAGATG 
CTAGTGACTG GAGGCATAAG CTCTGAGATG GAACACTATT TGGTTAAGGA GAAGCCCTAT 
AAGACAACTG ACGACTATGA GGCAATCAAG TCTGGGAGGA CGCGGTGTAA GCTGGGACTC 
CTCTCTTTAG ACCAAACCGT TCTATCAGGC ATTAATCCAA GAAAAACCTT TGGACAGCTG 
TTCTGTGGCT CATTGGATAA TTTCGGGATC CTATGTGTCG GCTTATACCG TATGATTGAT 
GAAGAGGAAC CCAGCCAAGA ACACAAAAGG TTTGTGATCA CCAGGCCATC CAATGAGTGC 
CACCTGCTGC CCTCAGATCT CGTGTTTTGT GCCATCCCTT TCAACACCAC CTGTGGCAAA 
TCAGACAGCA GTCCTTCAAT TCAGGCTCAA AACAACTCTA CAAACGCGAC GACGCCATTG 
GCCCAGGGGT CGAATTTCTT CGATTCGCAC CATGCCGACG AGTCCCACGA TCTTTACCCA 
GTCGACGACA CGGGAGAGAG GTGGTCTCAG CACCACCACT CCCGAGTCTA TCCTTTGGAC 
ACGTTAGATG CCAGTGATAT TGTTCAAGAA AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509087.1
CDS	33..3428
Translation	MSQTLLDSLNQKELTETSCTIEIQAAFILSSLATFFGGLIILFLFRIALKSSRSWKYVKGPRGLLELFSSRRIEANPLRKLYFHGVFRQRIEMLLSAQTVVGQVLVILVFVLSIGSLVIYFINSMDPVRRCSSYEDKIVHVDLSFNAFFSFYFGLRFWAAEDKIKFWLEMNSIVDIFTIPPTFISYYLKSNWLGLRFLRALRLLELPKILQILQVIKTSNSVKLSKLLSIVISTWFTAAGFLHLTFPLPSLVGQVENSGDPWLNGRNSQTMSYFESIYLVTATMSTVGFGDVVAKTSLGRIFIVFFTLGSLILFANYIPEMVELFSTRKKYTKPYEAVKGKKFIVVCGNITVDSVTAFLRNFLHWKSGEINIEIVFLGETLPCLELETLLKCHTSCTNFVCGTALKFEDLKRVAVENSEACLILANHFCSDLHDEDNSNIMRVLSIKNYYPQTRVIIQILQSQNKVFLSKIPNWDWSAGDNILCFAELKLGFIAQGCLVPGLCTFLTTLFIEQNQKVFPKHPWQKHFLNGLKNKILTQRLSNDFVGMTFPQVSRLCFVKLNLMLIAIQHKPFFHSCCTLILNPSSQVRLNKDTLGFFIADSSKAVKRAFFYCSNCHSDVCNPELIGKCNCKIKSRQQLIAPTIMVMKSSLTDFTTSSHIHASMSTEIHTCFSREQPSLITITTNRPTTNDTVDDTDMLDSSGMFHWCRAMPLDKVVLKRSEKAKHEFQNHIVVCVFGDAQCTLVGLRNFVMPLRASNYTRQELKDIVFIGSLEYFQREWRFLRNFPKIHIMPGSALYMGDLIAVNVEQCSMCVILATPYKALSSQILVDTEAIMATLNIQSLRITSPTPGSSKSEVKPSSAFDSKERKQRYKQIPILTELKNPSNIHFIEQMGGLDGMLKGTSLHLSTSFSTGAVFSDTFLDSLLATSFYNYHVVELLQMLVTGGISSEMEHYLVKEKPYKTTDDYEAIKSGRTRCKLGLLSLDQTVLSGINPRKTFGQLFCGSLDNFGILCVGLYRMIDEEEPSQEHKRFVITRPSNECHLLPSDLVFCAIPFNTTCGKSDSSPSIQAQNNSTNATTPLAQGSNFFDSHHADESHDLYPVDDTGERWSQHHHSRVYPLDTLDASDIVQEK

Target ORF information:

RefSeq Version	XM_006509024.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily U, member 1 (Kcnu1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509024.2

ORF Insert Sequence:

ATGTCTCAAA CATTGCTAGA CAGTTTAAAT CAGAAGGAGT TGACGGAAAC GTCATGTACA 
ATCGAAATCC AGGCAGCGTT CATTCTTTCC TCCTTGGCGA CTTTCTTCGG GGGACTCATC 
ATCTTATTCC TTTTCAGAAT AGCCTTGAAA AGCTCAAGAA GTTGGAAATA CGTCAAGGGG 
CCAAGAGGAC TCTTGGAACT ATTCTCATCA CGTAGAATCG AGGCTAATCC TTTGAGGAAA 
CTTTACTTTC ATGGAGTATT TCGTCAGCGC ATCGAAATGC TGCTTTCTGC ACAGACCGTC 
GTGGGGCAAG TGTTGGTGAT CCTTGTCTTT GTACTAAGCA TCGGGTCTCT TGTGATCTAT 
TTCATCAATT CAATGGATCC TGTTCGAAGG TGTTCTTCAT ATGAAGACAA AATTGTCCAT 
GTGGATTTGA GTTTCAACGC TTTCTTTAGC TTCTATTTTG GGTTGAGGTT TTGGGCAGCT 
GAAGACAAGA TCAAGTTCTG GTTGGAGATG AATTCAATTG TAGACATTTT TACCATCCCG 
CCAACCTTTA TTTCTTATTA TTTGAAGAGT AATTGGCTAG GTTTGAGATT TCTAAGAGCT 
CTGCGGTTGC TCGAACTCCC TAAAATCTTA CAGATCCTAC AAGTCATCAA GACCAGCAAT 
TCAGTGAAGC TTTCCAAACT GTTGTCAATA GTTATCAGTA CCTGGTTCAC GGCAGCAGGA 
TTCCTTCACC TGACCTTTCC TTTGCCATCT CTCGTTGGAC AGGTGGAAAA TTCTGGTGAC 
CCCTGGCTCA ACGGAAGAAA CTCACAGACT ATGTCATACT TTGAGTCTAT TTATCTGGTG 
ACAGCAACAA TGTCAACTGT TGGCTTTGGG GACGTGGTGG CCAAGACATC CCTAGGACGG 
ATTTTCATTG TTTTCTTCAC CCTTGGGAGT TTGATACTAT TTGCAAACTA CATTCCAGAA 
ATGGTGGAGC TCTTTTCTAC CAGGAAGAAA TACACCAAGC CCTACGAAGC AGTCAAAGGA 
AAAAAGTTCA TCGTGGTCTG TGGAAACATC ACAGTTGACA GTGTTACTGC TTTCCTGAGG 
AATTTTCTCC ACTGGAAGTC CGGGGAAATC AATATTGAGA TCGTATTCCT TGGAGAGACT 
CTCCCTTGCT TGGAACTGGA GACCTTACTG AAGTGCCACA CATCCTGTAC CAACTTCGTA 
TGCGGCACCG CACTGAAGTT CGAGGATCTG AAGCGAGTTG CAGTGGAGAA CTCGGAGGCG 
TGCCTGATTC TAGCCAACCA TTTCTGTAGT GACTTACATG ACGAAGACAA CTCAAACATT 
ATGAGGGTGC TCTCGATCAA GAACTATTAT CCACAGACCA GAGTCATCAT TCAGATACTT 
CAGTCTCAAA ACAAGGTTTT CCTGTCAAAA ATCCCCAACT GGGACTGGAG TGCTGGAGAC 
AATATCCTCT GCTTTGCAGA GCTAAAGCTC GGATTTATCG CCCAAGGCTG CTTGGTGCCA 
GGGCTGTGCA CCTTTCTCAC GACTCTGTTC ATTGAACAAA ACCAAAAGGT TTTTCCTAAA 
CATCCCTGGC AAAAACATTT CTTGAATGGC TTGAAGAACA AGATTCTGAC ACAGCGCCTC 
TCTAACGACT TCGTGGGGAT GACATTTCCC CAGGTCTCCC GGCTCTGCTT TGTGAAGCTA 
AATCTCATGC TGATCGCCAT CCAACACAAG CCCTTCTTTC ACAGTTGTTG CACTCTGATA 
CTAAACCCAT CATCCCAAGT GAGGCTGAAT AAGGACACCT TAGGGTTCTT CATTGCGGAC 
TCCTCCAAAG CCGTCAAAAG GGCTTTCTTT TACTGTTCCA ACTGTCACAG CGATGTGTGC 
AATCCTGAGC TAATTGGAAA GTGTAACTGT AAAATCAAGA GCCGACAACA ACTCATAGCA 
CCGACCATCA TGGTGATGAA AAGCAGCTTG ACCGATTTCA CCACTTCTTC ACACATCCAC 
GCTTCTATGT CAACAGAAAT TCACACTTGT TTTTCAAGAG AACAGCCTAG TTTGATCACC 
ATTACAACCA ACAGACCAAC GACAAACGAC ACAGTGGATG ATACCGACAT GCTGGACAGC 
AGTGGCATGT TTCACTGGTG CAGAGCAATG CCCTTGGACA AGGTGGTTCT GAAACGAAGT 
GAGAAGGCAA AACACGAGTT TCAGAACCAC ATTGTAGTAT GCGTGTTTGG AGATGCCCAA 
TGTACCCTGG TGGGGCTTCG GAATTTCGTG ATGCCCCTGA GAGCCAGCAA CTACACCCGG 
CAGGAGCTGA AGGACATTGT TTTTATTGGG TCTCTGGAGT ACTTCCAGAG AGAATGGCGA 
TTTCTCCGAA ACTTTCCCAA GATACACATT ATGCCTGGAT CTGCACTCTA CATGGGAGAT 
CTGATTGCAG TCAATGTAGA GCAGTGCTCT ATGTGCGTCA TCTTAGCCAC ACCCTACAAG 
GCACTGAGCA GCCAGATTCT GGTGGACACA GAGGCCATCA TGGCCACCCT CAACATCCAG 
TCCCTGCGGA TCACCAGTCC TACTCCAGGG TCTTCAAAGT CAGAAGTAAA GCCATCATCT 
GCCTTTGATA GTAAAGAAAG GAAGCAAAGA TACAAACAGA TCCCCATTCT CACTGAACTG 
AAGAATCCCT CCAACATCCA CTTTATTGAG CAGATGGGCG GACTGGATGG AATGCTCAAA 
GGGACTAGCT TGCATCTCAG CACTTCTTTC TCCACCGGTG CTGTCTTTTC AGACACCTTC 
TTGGATTCTC TCCTGGCCAC GTCCTTCTAC AATTACCATG TCGTGGAATT ACTTCAGATG 
CTAGTGACTG GAGGCATAAG CTCTGAGATG GAACACTATT TGGTTAAGGA GAAGCCCTAT 
AAGACAACTG ACGACTATGA GGCAATCAAG TCTGGGAGGA CGCGGTGTAA GCTGGGACTC 
CTCTCTTTAG ACCAAACCGT TCTATCAGGC ATTAATCCAA GAAAAACCTT TGGACAGCTG 
TTCTGTGGCT CATTGGATAA TTTCGGGATC CTATGTGTCG GCTTATACCG TATGATTGAT 
GAAGAGGAAC CCAGCCAAGA ACACAAAAGG TTTGTGATCA CCAGGCCATC CAATGAGTGC 
CACCTGCTGC CCTCAGATCT CGTGTTTTGT GCCATCCCTT TCAACACCAC CTGTGGCAAA 
TCAGACAGCA GTCCTTCAAT TCAGGCTCAA AACAACTCTA CAAACGCGAC GACGCCATTG 
GCCCAGGGGT CGAATTTCTT CGATTCGCAC CATGCCGACG AGTCCCACGA TCTTTACCCA 
GTCGACGACA CGGGAGAGAG GTGGTCTCAG CACCACCACT CCCGAGTCTA TCCTTTGGAC 
ACGTTAGATG CCAGTGATAT TGTTCAAGAA AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnu1 ( XM_017312583.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168072.1 Mus musculus potassium channel, subfamily U, member 1 (Kcnu1), transcript variant X4, mRNA.

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CloneID	OMu68043
Clone ID Related Accession (Same CDS sequence)	XM_017312583.1
Accession Version	XM_017312583.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	potassium channel subfamily U member 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTTT CTGCACAGAC CGTCGTGGGG CAAGTGTTGG TGATCCTTGT CTTTGTACTA 
AGCATCGGGT CTCTTGTGAT CTATTTCATC AATTCAATGG ATCCTGTTCG AAGGTGTTCT 
TCATATGAAG ACAAAATTGT CCATGTGGAT TTGAGTTTCA ACGCTTTCTT TAGCTTCTAT 
TTTGGGTTGA GGTTTTGGGC AGCTGAAGAC AAGATCAAGT TCTGGTTGGA GATGAATTCA 
ATTGTAGACA TTTTTACCAT CCCGCCAACC TTTATTTCTT ATTATTTGAA GAGTAATTGG 
CTAGGTTTGA GATTTCTAAG AGCTCTGCGG TTGCTCGAAC TCCCTAAAAT CTTACAGATC 
CTACAAGTCA TCAAGACCAG CAATTCAGTG AAGCTTTCCA AACTGTTGTC AATAGTTATC 
AGTACCTGGT TCACGGCAGC AGGATTCCTT CACCTGACCT TTCCTTTGCC ATCTCTCGTT 
GGACAGGTGG AAAATTCTGG TGACCCCTGG CTCAACGGAA GAAACTCACA GACTATGTCA 
TACTTTGAGT CTATTTATCT GGTGACAGCA ACAATGTCAA CTGTTGGCTT TGGGGACGTG 
GTGGCCAAGA CATCCCTAGG ACGGATTTTC ATTGTTTTCT TCACCCTTGG GAGTTTGATA 
CTATTTGCAA ACTACATTCC AGAAATGGTG GAGCTCTTTT CTACCAGGAA GAAATACACC 
AAGCCCTACG AAGCAGTCAA AGGAAAAAAG TTCATCGTGG TCTGTGGAAA CATCACAGTT 
GACAGTGTTA CTGCTTTCCT GAGGAATTTT CTCCACTGGA AGTCCGGGGA AATCAATATT 
GAGATCGTAT TCCTTGGAGA GACTCTCCCT TGCTTGGAAC TGGAGACCTT ACTGAAGTGC 
CACACATCCT GTACCAACTT CGTATGCGGC ACCGCACTGA AGTTCGAGGA TCTGAAGCGA 
GTTGCAGTGG AGAACTCGGA GGCGTGCCTG ATTCTAGCCA ACCATTTCTG TAGTGACTTA 
CATGACGAAG ACAACTCAAA CATTATGAGG GTGCTCTCGA TCAAGAACTA TTATCCACAG 
ACCAGAGTCA TCATTCAGAT ACTTCAGTCT CAAAACAAGG TTTTCCTGTC AAAAATCCCC 
AACTGGGACT GGAGTGCTGG AGACAATATC CTCTGCTTTG CAGAGCTAAA GCTCGGATTT 
ATCGCCCAAG GCTGCTTGGT GCCAGGGCTG TGCACCTTTC TCACGACTCT GTTCATTGAA 
CAAAACCAAA AGGTTTTTCC TAAACATCCC TGGCAAAAAC ATTTCTTGAA TGGCTTGAAG 
AACAAGATTC TGACACAGCG CCTCTCTAAC GACTTCGTGG GGATGACATT TCCCCAGGTC 
TCCCGGCTCT GCTTTGTGAA GCTAAATCTC ATGCTGATCG CCATCCAACA CAAGCCCTTC 
TTTCACAGTT GTTGCACTCT GATACTAAAC CCATCATCCC AAGTGAGGCT GAATAAGGAC 
ACCTTAGGGT TCTTCATTGC GGACTCCTCC AAAGCCGTCA AAAGGGCTTT CTTTTACTGT 
TCCAACTGTC ACAGCGATGT GTGCAATCCT GAGCTAATTG GAAAGTGTAA CTGTAAAATC 
AAGAGCCGAC AACAACTCAT AGCACCGACC ATCATGGTGA TGAAAAGCAG CTTGACCGAT 
TTCACCACTT CTTCACACAT CCACGCTTCT ATGTCAACAG AAATTCACAC TTGTTTTTCA 
AGAGAACAGC CTAGTTTGAT CACCATTACA ACCAACAGAC CAACGACAAA CGACACAGTG 
GATGATACCG ACATGCTGGA CAGCAGTGGC ATGTTTCACT GGTGCAGAGC AATGCCCTTG 
GACAAGGTGG TTCTGAAACG AAGTGAGAAG GCAAAACACG AGTTTCAGAA CCACATTGTA 
GTATGCGTGT TTGGAGATGC CCAATGTACC CTGGTGGGGC TTCGGAATTT CGTGATGCCC 
CTGAGAGCCA GCAACTACAC CCGGCAGGAG CTGAAGGACA TTGTTTTTAT TGGGTCTCTG 
GAGTACTTCC AGAGAGAATG GCGATTTCTC CGAAACTTTC CCAAGATACA CATTATGCCT 
GGATCTGCAC TCTACATGGG AGATCTGATT GCAGTCAATG TAGAGCAGTG CTCTATGTGC 
GTCATCTTAG CCACACCCTA CAAGGCACTG AGCAGCCAGA TTCTGGTGGA CACAGAGGCC 
ATCATGGCCA CCCTCAACAT CCAGTCCCTG CGGATCACCA GTCCTACTCC AGGGTCTTCA 
AAGTCAGAAG TAAAGCCATC ATCTGCCTTT GATAGTAAAG AAAGGAAGCA AAGATACAAA 
CAGATCCCCA TTCTCACTGA ACTGAAGAAT CCCTCCAACA TCCACTTTAT TGAGCAGATG 
GGCGGACTGG ATGGAATGCT CAAAGGGACT AGCTTGCATC TCAGCACTTC TTTCTCCACC 
GGTGCTGTCT TTTCAGACAC CTTCTTGGAT TCTCTCCTGG CCACGTCCTT CTACAATTAC 
CATGTCGTGG AATTACTTCA GATGCTAGTG ACTGGAGGCA TAAGCTCTGA GATGGAACAC 
TATTTGGTTA AGGAGAAGCC CTATAAGACA ACTGACGACT ATGAGGCAAT CAAGTCTGGG 
AGGACGCGGT GTAAGCTGGG ACTCCTCTCT TTAGACCAAA CCGTTCTATC AGGCATTAAT 
CCAAGAAAAA CCTTTGGACA GCTGTTCTGT GGCTCATTGG ATAATTTCGG GATCCTATGT 
GTCGGCTTAT ACCGTATGAT TGATGAAGAG GAACCCAGCC AAGAACACAA AAGGTTTGTG 
ATCACCAGGC CATCCAATGA GTGCCACCTG CTGCCCTCAG ATCTCGTGTT TTGTGCCATC 
CCTTTCAACA CCACCTGTGG CAAATCAGAC AGCAGTCCTT CAATTCAGGC TCAAAACAAC 
TCTACAAACG CGACGACGCC ATTGGCCCAG GGGTCGAATT TCTTCGATTC GCACCATGCC 
GACGAGTCCC ACGATCTTTA CCCAGTCGAC GACACGGGAG AGAGGTGGTC TCAGCACCAC 
CACTCCCGAG TCTATCCTTT GGACACGTTA GATGCCAGTG ATATTGTTCA AGAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168072.1
CDS	99..3218
Translation	MLLSAQTVVGQVLVILVFVLSIGSLVIYFINSMDPVRRCSSYEDKIVHVDLSFNAFFSFYFGLRFWAAEDKIKFWLEMNSIVDIFTIPPTFISYYLKSNWLGLRFLRALRLLELPKILQILQVIKTSNSVKLSKLLSIVISTWFTAAGFLHLTFPLPSLVGQVENSGDPWLNGRNSQTMSYFESIYLVTATMSTVGFGDVVAKTSLGRIFIVFFTLGSLILFANYIPEMVELFSTRKKYTKPYEAVKGKKFIVVCGNITVDSVTAFLRNFLHWKSGEINIEIVFLGETLPCLELETLLKCHTSCTNFVCGTALKFEDLKRVAVENSEACLILANHFCSDLHDEDNSNIMRVLSIKNYYPQTRVIIQILQSQNKVFLSKIPNWDWSAGDNILCFAELKLGFIAQGCLVPGLCTFLTTLFIEQNQKVFPKHPWQKHFLNGLKNKILTQRLSNDFVGMTFPQVSRLCFVKLNLMLIAIQHKPFFHSCCTLILNPSSQVRLNKDTLGFFIADSSKAVKRAFFYCSNCHSDVCNPELIGKCNCKIKSRQQLIAPTIMVMKSSLTDFTTSSHIHASMSTEIHTCFSREQPSLITITTNRPTTNDTVDDTDMLDSSGMFHWCRAMPLDKVVLKRSEKAKHEFQNHIVVCVFGDAQCTLVGLRNFVMPLRASNYTRQELKDIVFIGSLEYFQREWRFLRNFPKIHIMPGSALYMGDLIAVNVEQCSMCVILATPYKALSSQILVDTEAIMATLNIQSLRITSPTPGSSKSEVKPSSAFDSKERKQRYKQIPILTELKNPSNIHFIEQMGGLDGMLKGTSLHLSTSFSTGAVFSDTFLDSLLATSFYNYHVVELLQMLVTGGISSEMEHYLVKEKPYKTTDDYEAIKSGRTRCKLGLLSLDQTVLSGINPRKTFGQLFCGSLDNFGILCVGLYRMIDEEEPSQEHKRFVITRPSNECHLLPSDLVFCAIPFNTTCGKSDSSPSIQAQNNSTNATTPLAQGSNFFDSHHADESHDLYPVDDTGERWSQHHHSRVYPLDTLDASDIVQEK

Target ORF information:

RefSeq Version	XM_017312583.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium channel, subfamily U, member 1 (Kcnu1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312583.1

ORF Insert Sequence:

ATGCTGCTTT CTGCACAGAC CGTCGTGGGG CAAGTGTTGG TGATCCTTGT CTTTGTACTA 
AGCATCGGGT CTCTTGTGAT CTATTTCATC AATTCAATGG ATCCTGTTCG AAGGTGTTCT 
TCATATGAAG ACAAAATTGT CCATGTGGAT TTGAGTTTCA ACGCTTTCTT TAGCTTCTAT 
TTTGGGTTGA GGTTTTGGGC AGCTGAAGAC AAGATCAAGT TCTGGTTGGA GATGAATTCA 
ATTGTAGACA TTTTTACCAT CCCGCCAACC TTTATTTCTT ATTATTTGAA GAGTAATTGG 
CTAGGTTTGA GATTTCTAAG AGCTCTGCGG TTGCTCGAAC TCCCTAAAAT CTTACAGATC 
CTACAAGTCA TCAAGACCAG CAATTCAGTG AAGCTTTCCA AACTGTTGTC AATAGTTATC 
AGTACCTGGT TCACGGCAGC AGGATTCCTT CACCTGACCT TTCCTTTGCC ATCTCTCGTT 
GGACAGGTGG AAAATTCTGG TGACCCCTGG CTCAACGGAA GAAACTCACA GACTATGTCA 
TACTTTGAGT CTATTTATCT GGTGACAGCA ACAATGTCAA CTGTTGGCTT TGGGGACGTG 
GTGGCCAAGA CATCCCTAGG ACGGATTTTC ATTGTTTTCT TCACCCTTGG GAGTTTGATA 
CTATTTGCAA ACTACATTCC AGAAATGGTG GAGCTCTTTT CTACCAGGAA GAAATACACC 
AAGCCCTACG AAGCAGTCAA AGGAAAAAAG TTCATCGTGG TCTGTGGAAA CATCACAGTT 
GACAGTGTTA CTGCTTTCCT GAGGAATTTT CTCCACTGGA AGTCCGGGGA AATCAATATT 
GAGATCGTAT TCCTTGGAGA GACTCTCCCT TGCTTGGAAC TGGAGACCTT ACTGAAGTGC 
CACACATCCT GTACCAACTT CGTATGCGGC ACCGCACTGA AGTTCGAGGA TCTGAAGCGA 
GTTGCAGTGG AGAACTCGGA GGCGTGCCTG ATTCTAGCCA ACCATTTCTG TAGTGACTTA 
CATGACGAAG ACAACTCAAA CATTATGAGG GTGCTCTCGA TCAAGAACTA TTATCCACAG 
ACCAGAGTCA TCATTCAGAT ACTTCAGTCT CAAAACAAGG TTTTCCTGTC AAAAATCCCC 
AACTGGGACT GGAGTGCTGG AGACAATATC CTCTGCTTTG CAGAGCTAAA GCTCGGATTT 
ATCGCCCAAG GCTGCTTGGT GCCAGGGCTG TGCACCTTTC TCACGACTCT GTTCATTGAA 
CAAAACCAAA AGGTTTTTCC TAAACATCCC TGGCAAAAAC ATTTCTTGAA TGGCTTGAAG 
AACAAGATTC TGACACAGCG CCTCTCTAAC GACTTCGTGG GGATGACATT TCCCCAGGTC 
TCCCGGCTCT GCTTTGTGAA GCTAAATCTC ATGCTGATCG CCATCCAACA CAAGCCCTTC 
TTTCACAGTT GTTGCACTCT GATACTAAAC CCATCATCCC AAGTGAGGCT GAATAAGGAC 
ACCTTAGGGT TCTTCATTGC GGACTCCTCC AAAGCCGTCA AAAGGGCTTT CTTTTACTGT 
TCCAACTGTC ACAGCGATGT GTGCAATCCT GAGCTAATTG GAAAGTGTAA CTGTAAAATC 
AAGAGCCGAC AACAACTCAT AGCACCGACC ATCATGGTGA TGAAAAGCAG CTTGACCGAT 
TTCACCACTT CTTCACACAT CCACGCTTCT ATGTCAACAG AAATTCACAC TTGTTTTTCA 
AGAGAACAGC CTAGTTTGAT CACCATTACA ACCAACAGAC CAACGACAAA CGACACAGTG 
GATGATACCG ACATGCTGGA CAGCAGTGGC ATGTTTCACT GGTGCAGAGC AATGCCCTTG 
GACAAGGTGG TTCTGAAACG AAGTGAGAAG GCAAAACACG AGTTTCAGAA CCACATTGTA 
GTATGCGTGT TTGGAGATGC CCAATGTACC CTGGTGGGGC TTCGGAATTT CGTGATGCCC 
CTGAGAGCCA GCAACTACAC CCGGCAGGAG CTGAAGGACA TTGTTTTTAT TGGGTCTCTG 
GAGTACTTCC AGAGAGAATG GCGATTTCTC CGAAACTTTC CCAAGATACA CATTATGCCT 
GGATCTGCAC TCTACATGGG AGATCTGATT GCAGTCAATG TAGAGCAGTG CTCTATGTGC 
GTCATCTTAG CCACACCCTA CAAGGCACTG AGCAGCCAGA TTCTGGTGGA CACAGAGGCC 
ATCATGGCCA CCCTCAACAT CCAGTCCCTG CGGATCACCA GTCCTACTCC AGGGTCTTCA 
AAGTCAGAAG TAAAGCCATC ATCTGCCTTT GATAGTAAAG AAAGGAAGCA AAGATACAAA 
CAGATCCCCA TTCTCACTGA ACTGAAGAAT CCCTCCAACA TCCACTTTAT TGAGCAGATG 
GGCGGACTGG ATGGAATGCT CAAAGGGACT AGCTTGCATC TCAGCACTTC TTTCTCCACC 
GGTGCTGTCT TTTCAGACAC CTTCTTGGAT TCTCTCCTGG CCACGTCCTT CTACAATTAC 
CATGTCGTGG AATTACTTCA GATGCTAGTG ACTGGAGGCA TAAGCTCTGA GATGGAACAC 
TATTTGGTTA AGGAGAAGCC CTATAAGACA ACTGACGACT ATGAGGCAAT CAAGTCTGGG 
AGGACGCGGT GTAAGCTGGG ACTCCTCTCT TTAGACCAAA CCGTTCTATC AGGCATTAAT 
CCAAGAAAAA CCTTTGGACA GCTGTTCTGT GGCTCATTGG ATAATTTCGG GATCCTATGT 
GTCGGCTTAT ACCGTATGAT TGATGAAGAG GAACCCAGCC AAGAACACAA AAGGTTTGTG 
ATCACCAGGC CATCCAATGA GTGCCACCTG CTGCCCTCAG ATCTCGTGTT TTGTGCCATC 
CCTTTCAACA CCACCTGTGG CAAATCAGAC AGCAGTCCTT CAATTCAGGC TCAAAACAAC 
TCTACAAACG CGACGACGCC ATTGGCCCAG GGGTCGAATT TCTTCGATTC GCACCATGCC 
GACGAGTCCC ACGATCTTTA CCCAGTCGAC GACACGGGAG AGAGGTGGTC TCAGCACCAC 
CACTCCCGAG TCTATCCTTT GGACACGTTA GATGCCAGTG ATATTGTTCA AGAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transient exposure to calcium ionophore enables in vitro fertilization in sterile mouse models.
Scientific reports633589(2016 Sep)
Navarrete FA,Alvau A,Lee HC,Levin LR,Buck J,Leon PM,Santi CM,Krapf D,Mager J,Fissore RA,Salicioni AM,Darszon A,Visconti PE

Src Kinase Is the Connecting Player between Protein Kinase A (PKA) Activation and Hyperpolarization through SLO3 Potassium Channel Regulation in Mouse Sperm.
The Journal of biological chemistry290(30)18855-64(2015 Jul)
Stival C,La Spina FA,Bar? Graf C,Arcelay E,Arranz SE,Ferreira JJ,Le Grand S,Dzikunu VA,Santi CM,Visconti PE,Buffone MG,Krapf D

Flow cytometry analysis reveals that only a subpopulation of mouse sperm undergoes hyperpolarization during capacitation.
Biology of reproduction92(5)121(2015 May)
Escoffier J,Navarrete F,Haddad D,Santi CM,Darszon A,Visconti PE

SLO3 auxiliary subunit LRRC52 controls gating of sperm KSPER currents and is critical for normal fertility.
Proceedings of the National Academy of Sciences of the United States of America112(8)2599-604(2015 Feb)
Zeng XH,Yang C,Xia XM,Liu M,Lingle CJ

SLO3 K+ channels control calcium entry through CATSPER channels in sperm.
The Journal of biological chemistry289(46)32266-75(2014 Nov)
Ch?vez JC,Ferreira JJ,Butler A,De La Vega Beltr?n JL,Trevi?o CL,Darszon A,Salkoff L,Santi CM

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Kcnu1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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