Ercc3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ercc3
Entrez Gene ID	13872
Full Name	excision repair cross-complementing rodent repair deficiency, complementation group 3
Synonyms	BTF2 p89,Ercc-3,XPB
General protein information	Preferred Names excision repair cross-complementing rodent repair deficiency, complementation group 3 Names TFIIH basal transcription factor complex helicase XPB subunit BTF2-p89 DNA excision repair protein ERCC-3 DNA repair protein complementing XP-B cells TFIIH 89 kDa subunit basic transcription factor 2 89 kDa subunit excision repair 3 xeroderma pigmentosum group B-complementing protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18\|18 B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_133658.1	NP_598419.1	TFIIH basal transcription factor complex helicase XPB subunit
NM_133658.2	NP_598419.1	general transcription and DNA repair factor IIH helicase subunit XPB
XM_006525636.2	XP_006525699.1	general transcription and DNA repair factor IIH helicase subunit XPB isoform X1

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	Y66D12A.15	NP_499487.1
Eremothecium gossypii	AGOS_AFR280W	NP_985827.1
Arabidopsis thaliana (thale cress)	XPB1	NP_568592.1
Xenopus tropicalis (tropical clawed frog)	ercc3	NP_001123812.1
Canis lupus familiaris (dog)	ERCC3	XP_533314.2
Rattus norvegicus (Norway rat)	Ercc3	NP_001026814.1
Gallus gallus (chicken)	ERCC3	NP_001006523.1
Saccharomyces cerevisiae (baker's yeast)	SSL2	NP_012123.1
Arabidopsis thaliana (thale cress)	XPB2	NP_568591.1
Oryza sativa (rice)	Os01g0691600	NP_001043936.1
Pan troglodytes (chimpanzee)	ERCC3	XP_525907.3
Drosophila melanogaster (fruit fly)	hay	NP_524020.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012169	XP_320364.4
Homo sapiens (human)	ERCC3	NP_000113.1
Bos taurus (cattle)	ERCC3	NP_001039453.1
Mus musculus (house mouse)	Ercc3	NP_598419.1
Danio rerio (zebrafish)	ercc3	NP_963876.1
Kluyveromyces lactis	KLLA0E13025g	XP_454537.1
	ERCC3	XP_001086722.1
Schizosaccharomyces pombe (fission yeast)	ptr8	NP_593474.1
Magnaporthe oryzae (rice blast fungus)	MGG_06470	XP_003717094.1
Neurospora crassa	NCU06438	XP_957329.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

ELL, a novel TFIIH partner, is involved in transcription restart after DNA repair.
Mourgues S, Gautier V, Lagarou A, Bordier C, Mourcet A, Slingerland J, Kaddoum L, Coin F, Vermeulen W, Gonzales de Peredo A, Monsarrat B, Mari PO, Giglia-Mari G
Proceedings of the National Academy of Sciences of the United States of America110(44)17927-32(2013 Oct)

Mutations in TFIIH causing trichothiodystrophy are responsible for defects in ribosomal RNA production and processing.
Nonnekens J, Perez-Fernandez J, Theil AF, Gadal O, Bonnart C, Giglia-Mari G
Human molecular genetics22(14)2881-93(2013 Jul)

Generation of DNA single-strand displacement by compromised nucleotide excision repair.
Godon C, Mourgues S, Nonnekens J, Mourcet A, Coin F, Vermeulen W, Mari PO, Giglia-Mari G
The EMBO journal31(17)3550-63(2012 Aug)

Defective transcription initiation causes postnatal growth failure in a mouse model of nucleotide excision repair (NER) progeria.
Kamileri I, Karakasilioti I, Sideri A, Kosteas T, Tatarakis A, Talianidis I, Garinis GA
Proceedings of the National Academy of Sciences of the United States of America109(8)2995-3000(2012 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ercc3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ercc3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu54385	XM_006525636.2 Latest version!	Mus musculus excision repair cross-complementing rodent repair deficiency, complementation group 3 (Ercc3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu22187	NM_133658.2 Latest version!	Mus musculus excision repair cross-complementing rodent repair deficiency, complementation group 3 (Ercc3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$461.30 $659.00
View Old Accession Versions >>
OMu22187	NM_133658.1	Mus musculus excision repair cross-complementing rodent repair deficiency, complementation group 3 (Ercc3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$461.30 $659.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ercc3 ( XM_006525636.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525699.1 Mus musculus excision repair cross-complementing rodent repair deficiency, complementation group 3 (Ercc3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu54385
Clone ID Related Accession (Same CDS sequence)	XM_006525636.2
Accession Version	XM_006525636.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	general transcription and DNA repair factor IIH helicase subunit XPB isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006525636.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAAAA GAGATCGAGT GGACCGCGAC AAAAAGAAAT CCAAGAAGAG GCAGTATGAA 
GAGGAAGAGG AAGACGAAGA CGACATTCCT GGGAACGAGT CTCAGGAGGC GGTGCCCTCC 
GCCGCTGGGA AACAGGTGGA CGAGTCCAGC ACCAAAGTGG ATGAGTATGG AGCAAAGGAC 
TACAGACAGC AGATGCCACT AAAGGGTGAC CATACCTCCA GGCCCCTCTG GGTGGCTCCT 
GATGGCCACA TTTTCTTGGA AGCCTTCTCT CCAGTTTACA AATATGCCCA AGACTTCCTG 
GTGGCAATTG CAGAGCCGGT GTGCCGGCCT ACTCATGTAC ATGAATACAA GCTAACCGCC 
TACTCCCTCT ATGCAGCGGT CAGTGTTGGG CTGCAAACCA GTGACATTAC TGAGTACCTC 
AGAAAGCTCA GTAAGACTGG AGTTCCTGAT GGAATTATCC AGTTTATTAA GCTGTGCACT 
GTCAGCTATG GGAAGGTCAA GCTGGTCCTC AAGCACAACA GGTACTTTGT TGAAAGTTCC 
CACCCTGATG TTATCCAGCA TCTTCTTCAA GACCCAGTGA TCCGGGAATG TCGCTTGAGG 
AACGCCGAGG GCGAGGCCAC CGAACTCATC ACAGAGACTT TTACAAGCAA ATCTGCTATT 
TCTAAGACGG CGGCGGAAGG CAGTGGCGGG CCTTCTACTT CGCAGGGGGT AGATGCACAG 
GCCACGTCTG ACATACCCAA AGACCTGTTT GATTTTTATG AGCAAATGGA CAAGGATGAG 
GAGGAGGAGG AAGAGACACA GACAGTGTCC TTTGAAGTTA AGCAGGAAAT GATCGAGGAG 
CTGCAGAAGC GCTGCATCTG CTTAGAGTAC CCGCTGCTGG CAGAGTATGA CTTCCGGAAT 
GACACTCTCA ATCCTGACAT CAACATTGAC CTGAAGCCCA CAGCCGTACT CAGACCCTAT 
CAGGAGAAGA GCCTGCGGAA GATGTTTGGG AACGGACGAG CACGCTCAGG AGTCATTGTT 
CTTCCCTGTG GTGCTGGGAA GTCCCTGGTC GGCGTGACTG CCGCATGCAC TGTCAGAAAG 
CGCTGTCTCG TCCTGGGCAA CTCGGCTGTG TCTGTGGAGC AGTGGAAAGC CCAGTTTAAG 
ATGTGGTCAA CCATCGATGA CAGCCAGATC TGCCGCTTCA CCTCAGATGC CAAGGACAAG 
CCCATCGGCT GCTCCGTTGC CATTAGCACT TACTCTATGC TGGGCCACAC CACCAAAAGG 
TCCTGGGAAG CTGAGAGAGT CATGGAGTGG CTCAAAACCC AGGAGTGGGG CCTCATGATC 
CTTGACGAGG TGCACACCAT TCCAGCCAGG ATGTTCCGGC GGGTTCTGAC TATTGTGCAG 
GCGCACTGTA AGCTTGGTTT GACTGCAACC CTTGTCCGGG AAGATGACAA AATTGTTGAC 
TTAAATTTCC TGATTGGGCC CAAGCTCTAT GAAGCCAACT GGATGGAGCT GCAGAACAAT 
GGGTACATCG CTAAAGTCCA GTGTGCTGAG GTTTGGTGCC CGATGTCTCC CGAGTTCTAC 
CGAGAGTATG TGGCAATCAA AACAAAGAAA CGGATCCTGT TGTACACCAT GAATCCCAAC 
AAATTCAGAG CCTGCCAGTT TCTGATCAAG TTTCATGAAA GGAGGAATGA CAAGATTATT 
GTCTTTGCTG ACAACGTATT TGCCTTGAAG GAATATGCCA TTCGGCTGAA CAAGTTGGTG 
ACACATCCTT TGATCTGCCA GAAGCCAATG TCCTCATTCA GATCTCTTCC CATGGTGGCT 
CCCGACGGCA GGAAGCCCAG AGACTGGGAC GGGTACTCAG AGCCAAGAAA GGGATGGTCG 
CAGAGGAATA CAATGCCTTT TTCTACTCCC TGGTATCTCA GGACACACAG GAAATGGCTT 
ATTCTACCAA GCGACAGAGA TTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525699.1
CDS	91..2037
Translation	MGKRDRVDRDKKKSKKRQYEEEEEDEDDIPGNESQEAVPSAAGKQVDESSTKVDEYGAKDYRQQMPLKGDHTSRPLWVAPDGHIFLEAFSPVYKYAQDFLVAIAEPVCRPTHVHEYKLTAYSLYAAVSVGLQTSDITEYLRKLSKTGVPDGIIQFIKLCTVSYGKVKLVLKHNRYFVESSHPDVIQHLLQDPVIRECRLRNAEGEATELITETFTSKSAISKTAAEGSGGPSTSQGVDAQATSDIPKDLFDFYEQMDKDEEEEEETQTVSFEVKQEMIEELQKRCICLEYPLLAEYDFRNDTLNPDINIDLKPTAVLRPYQEKSLRKMFGNGRARSGVIVLPCGAGKSLVGVTAACTVRKRCLVLGNSAVSVEQWKAQFKMWSTIDDSQICRFTSDAKDKPIGCSVAISTYSMLGHTTKRSWEAERVMEWLKTQEWGLMILDEVHTIPARMFRRVLTIVQAHCKLGLTATLVREDDKIVDLNFLIGPKLYEANWMELQNNGYIAKVQCAEVWCPMSPEFYREYVAIKTKKRILLYTMNPNKFRACQFLIKFHERRNDKIIVFADNVFALKEYAIRLNKLVTHPLICQKPMSSFRSLPMVAPDGRKPRDWDGYSEPRKGWSQRNTMPFSTPWYLRTHRKWLILPSDRDS

Target ORF information:

RefSeq Version	XM_006525636.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus excision repair cross-complementing rodent repair deficiency, complementation group 3 (Ercc3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525636.2

ORF Insert Sequence:

ATGGGCAAAA GAGATCGAGT GGACCGCGAC AAAAAGAAAT CCAAGAAGAG GCAGTATGAA 
GAGGAAGAGG AAGACGAAGA CGACATTCCT GGGAACGAGT CTCAGGAGGC GGTGCCCTCC 
GCCGCTGGGA AACAGGTGGA CGAGTCCAGC ACCAAAGTGG ATGAGTATGG AGCAAAGGAC 
TACAGACAGC AGATGCCACT AAAGGGTGAC CATACCTCCA GGCCCCTCTG GGTGGCTCCT 
GATGGCCACA TTTTCTTGGA AGCCTTCTCT CCAGTTTACA AATATGCCCA AGACTTCCTG 
GTGGCAATTG CAGAGCCGGT GTGCCGGCCT ACTCATGTAC ATGAATACAA GCTAACCGCC 
TACTCCCTCT ATGCAGCGGT CAGTGTTGGG CTGCAAACCA GTGACATTAC TGAGTACCTC 
AGAAAGCTCA GTAAGACTGG AGTTCCTGAT GGAATTATCC AGTTTATTAA GCTGTGCACT 
GTCAGCTATG GGAAGGTCAA GCTGGTCCTC AAGCACAACA GGTACTTTGT TGAAAGTTCC 
CACCCTGATG TTATCCAGCA TCTTCTTCAA GACCCAGTGA TCCGGGAATG TCGCTTGAGG 
AACGCCGAGG GCGAGGCCAC CGAACTCATC ACAGAGACTT TTACAAGCAA ATCTGCTATT 
TCTAAGACGG CGGCGGAAGG CAGTGGCGGG CCTTCTACTT CGCAGGGGGT AGATGCACAG 
GCCACGTCTG ACATACCCAA AGACCTGTTT GATTTTTATG AGCAAATGGA CAAGGATGAG 
GAGGAGGAGG AAGAGACACA GACAGTGTCC TTTGAAGTTA AGCAGGAAAT GATCGAGGAG 
CTGCAGAAGC GCTGCATCTG CTTAGAGTAC CCGCTGCTGG CAGAGTATGA CTTCCGGAAT 
GACACTCTCA ATCCTGACAT CAACATTGAC CTGAAGCCCA CAGCCGTACT CAGACCCTAT 
CAGGAGAAGA GCCTGCGGAA GATGTTTGGG AACGGACGAG CACGCTCAGG AGTCATTGTT 
CTTCCCTGTG GTGCTGGGAA GTCCCTGGTC GGCGTGACTG CCGCATGCAC TGTCAGAAAG 
CGCTGTCTCG TCCTGGGCAA CTCGGCTGTG TCTGTGGAGC AGTGGAAAGC CCAGTTTAAG 
ATGTGGTCAA CCATCGATGA CAGCCAGATC TGCCGCTTCA CCTCAGATGC CAAGGACAAG 
CCCATCGGCT GCTCCGTTGC CATTAGCACT TACTCTATGC TGGGCCACAC CACCAAAAGG 
TCCTGGGAAG CTGAGAGAGT CATGGAGTGG CTCAAAACCC AGGAGTGGGG CCTCATGATC 
CTTGACGAGG TGCACACCAT TCCAGCCAGG ATGTTCCGGC GGGTTCTGAC TATTGTGCAG 
GCGCACTGTA AGCTTGGTTT GACTGCAACC CTTGTCCGGG AAGATGACAA AATTGTTGAC 
TTAAATTTCC TGATTGGGCC CAAGCTCTAT GAAGCCAACT GGATGGAGCT GCAGAACAAT 
GGGTACATCG CTAAAGTCCA GTGTGCTGAG GTTTGGTGCC CGATGTCTCC CGAGTTCTAC 
CGAGAGTATG TGGCAATCAA AACAAAGAAA CGGATCCTGT TGTACACCAT GAATCCCAAC 
AAATTCAGAG CCTGCCAGTT TCTGATCAAG TTTCATGAAA GGAGGAATGA CAAGATTATT 
GTCTTTGCTG ACAACGTATT TGCCTTGAAG GAATATGCCA TTCGGCTGAA CAAGTTGGTG 
ACACATCCTT TGATCTGCCA GAAGCCAATG TCCTCATTCA GATCTCTTCC CATGGTGGCT 
CCCGACGGCA GGAAGCCCAG AGACTGGGAC GGGTACTCAG AGCCAAGAAA GGGATGGTCG 
CAGAGGAATA CAATGCCTTT TTCTACTCCC TGGTATCTCA GGACACACAG GAAATGGCTT 
ATTCTACCAA GCGACAGAGA TTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ercc3 ( NM_133658.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598419.1 Mus musculus excision repair cross-complementing rodent repair deficiency, complementation group 3 (Ercc3), mRNA.

Price & Availability↑

CloneID	OMu22187
Clone ID Related Accession (Same CDS sequence)	NM_133658.1 , NM_133658.2
Accession Version	NM_133658.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-25
Organism	Mus musculus(house mouse)
Product	TFIIH basal transcription factor complex helicase XPB subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from S71186.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK151188.1, AK152347.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGGGCAAAA GAGATCGAGT GGACCGCGAC AAAAAGAAAT CCAAGAAGAG GCAGTATGAA 
GAGGAAGAGG AAGACGAAGA CGACATTCCT GGGAACGAGT CTCAGGAGGC GGTGCCCTCC 
GCCGCTGGGA AACAGGTGGA CGAGTCCAGC ACCAAAGTGG ATGAGTATGG AGCAAAGGAC 
TACAGACAGC AGATGCCACT AAAGGGTGAC CATACCTCCA GGCCCCTCTG GGTGGCTCCT 
GATGGCCACA TTTTCTTGGA AGCCTTCTCT CCAGTTTACA AATATGCCCA AGACTTCCTG 
GTGGCAATTG CAGAGCCGGT GTGCCGGCCT ACTCATGTAC ATGAATACAA GCTAACCGCC 
TACTCCCTCT ATGCAGCGGT CAGTGTTGGG CTGCAAACCA GTGACATTAC TGAGTACCTC 
AGAAAGCTCA GTAAGACTGG AGTTCCTGAT GGAATTATCC AGTTTATTAA GCTGTGCACT 
GTCAGCTATG GGAAGGTCAA GCTGGTCCTC AAGCACAACA GGTACTTTGT TGAAAGTTCC 
CACCCTGATG TTATCCAGCA TCTTCTTCAA GACCCAGTGA TCCGGGAATG TCGCTTGAGG 
AACGCCGAGG GCGAGGCCAC CGAACTCATC ACAGAGACTT TTACAAGCAA ATCTGCTATT 
TCTAAGACGG CGGCGGAAGG CAGTGGCGGG CCTTCTACTT CGCAGGGGGT AGATGCACAG 
GCCACGTCTG ACATACCCAA AGACCTGTTT GATTTTTATG AGCAAATGGA CAAGGATGAG 
GAGGAGGAGG AAGAGACACA GACAGTGTCC TTTGAAGTTA AGCAGGAAAT GATCGAGGAG 
CTGCAGAAGC GCTGCATCTG CTTAGAGTAC CCGCTGCTGG CAGAGTATGA CTTCCGGAAT 
GACACTCTCA ATCCTGACAT CAACATTGAC CTGAAGCCCA CAGCCGTACT CAGACCCTAT 
CAGGAGAAGA GCCTGCGGAA GATGTTTGGG AACGGACGAG CACGCTCAGG AGTCATTGTT 
CTTCCCTGTG GTGCTGGGAA GTCCCTGGTC GGCGTGACTG CCGCATGCAC TGTCAGAAAG 
CGCTGTCTCG TCCTGGGCAA CTCGGCTGTG TCTGTGGAGC AGTGGAAAGC CCAGTTTAAG 
ATGTGGTCAA CCATCGATGA CAGCCAGATC TGCCGCTTCA CCTCAGATGC CAAGGACAAG 
CCCATCGGCT GCTCCGTTGC CATTAGCACT TACTCTATGC TGGGCCACAC CACCAAAAGG 
TCCTGGGAAG CTGAGAGAGT CATGGAGTGG CTCAAAACCC AGGAGTGGGG CCTCATGATC 
CTTGACGAGG TGCACACCAT TCCAGCCAGG ATGTTCCGGC GGGTTCTGAC TATTGTGCAG 
GCGCACTGTA AGCTTGGTTT GACTGCAACC CTTGTCCGGG AAGATGACAA AATTGTTGAC 
TTAAATTTCC TGATTGGGCC CAAGCTCTAT GAAGCCAACT GGATGGAGCT GCAGAACAAT 
GGGTACATCG CTAAAGTCCA GTGTGCTGAG GTTTGGTGCC CGATGTCTCC CGAGTTCTAC 
CGAGAGTATG TGGCAATCAA AACAAAGAAA CGGATCCTGT TGTACACCAT GAATCCCAAC 
AAATTCAGAG CCTGCCAGTT TCTGATCAAG TTTCATGAAA GGAGGAATGA CAAGATTATT 
GTCTTTGCTG ACAACGTATT TGCCTTGAAG GAATATGCCA TTCGGCTGAA CAAACCTTAT 
ATCTACGGGC CCACGTCCCA GGGAGAACGT ATGCAGATTC TCCAGAACTT CAAGCACAAC 
CCCAAAATCA ACACCATCTT CATCTCCAAG GTTGGTGACA CATCCTTTGA TCTGCCAGAA 
GCCAATGTCC TCATTCAGAT CTCTTCCCAT GGTGGCTCCC GACGGCAGGA AGCCCAGAGA 
CTGGGACGGG TACTCAGAGC CAAGAAAGGG ATGGTCGCAG AGGAATACAA TGCCTTTTTC 
TACTCCCTGG TATCTCAGGA CACACAGGAA ATGGCTTATT CTACCAAGCG ACAGAGATTC 
CTAGTGGATC AAGGTTACAG CTTTAAGGTA ATCACAAAGC TAGCTGGCAT GGAGGAAGAG 
GAGCTGGCGT TCTCCACCAA AGAGGAGCAG CAGCAGCTCC TGCAGAAGGT GCTGGCAGCC 
ACCGACTTGG ATGCAGAGGA GGAAGTGGTG GCTGGAGAGT TTGGCTCTAG ATCTGGCCAG 
GCATCCCGAC GCTGTGGCAC CATGAGCTCC CTGTCAGGCG CAGACGACAC CGTGTACATG 
GAGTACCACT CCTCCCGAAG CAAGGCCTCC AGCAAGCACG TGCACCCGCT TTTCAAACGC 
TTCAGGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598419.1
CDS	64..2415
Translation	MGKRDRVDRDKKKSKKRQYEEEEEDEDDIPGNESQEAVPSAAGKQVDESSTKVDEYGAKDYRQQMPLKGDHTSRPLWVAPDGHIFLEAFSPVYKYAQDFLVAIAEPVCRPTHVHEYKLTAYSLYAAVSVGLQTSDITEYLRKLSKTGVPDGIIQFIKLCTVSYGKVKLVLKHNRYFVESSHPDVIQHLLQDPVIRECRLRNAEGEATELITETFTSKSAISKTAAEGSGGPSTSQGVDAQATSDIPKDLFDFYEQMDKDEEEEEETQTVSFEVKQEMIEELQKRCICLEYPLLAEYDFRNDTLNPDINIDLKPTAVLRPYQEKSLRKMFGNGRARSGVIVLPCGAGKSLVGVTAACTVRKRCLVLGNSAVSVEQWKAQFKMWSTIDDSQICRFTSDAKDKPIGCSVAISTYSMLGHTTKRSWEAERVMEWLKTQEWGLMILDEVHTIPARMFRRVLTIVQAHCKLGLTATLVREDDKIVDLNFLIGPKLYEANWMELQNNGYIAKVQCAEVWCPMSPEFYREYVAIKTKKRILLYTMNPNKFRACQFLIKFHERRNDKIIVFADNVFALKEYAIRLNKPYIYGPTSQGERMQILQNFKHNPKINTIFISKVGDTSFDLPEANVLIQISSHGGSRRQEAQRLGRVLRAKKGMVAEEYNAFFYSLVSQDTQEMAYSTKRQRFLVDQGYSFKVITKLAGMEEEELAFSTKEEQQQLLQKVLAATDLDAEEEVVAGEFGSRSGQASRRCGTMSSLSGADDTVYMEYHSSRSKASSKHVHPLFKRFRK

Target ORF information:

RefSeq Version	NM_133658.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus excision repair cross-complementing rodent repair deficiency, complementation group 3 (Ercc3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133658.1

ORF Insert Sequence:

ATGGGCAAAA GAGATCGAGT GGACCGCGAC AAAAAGAAAT CCAAGAAGAG GCAGTATGAA 
GAGGAAGAGG AAGACGAAGA CGACATTCCT GGGAACGAGT CTCAGGAGGC GGTGCCCTCC 
GCCGCTGGGA AACAGGTGGA CGAGTCCAGC ACCAAAGTGG ATGAGTATGG AGCAAAGGAC 
TACAGACAGC AGATGCCACT AAAGGGTGAC CATACCTCCA GGCCCCTCTG GGTGGCTCCT 
GATGGCCACA TTTTCTTGGA AGCCTTCTCT CCAGTTTACA AATATGCCCA AGACTTCCTG 
GTGGCAATTG CAGAGCCGGT GTGCCGGCCT ACTCATGTAC ATGAATACAA GCTAACCGCC 
TACTCCCTCT ATGCAGCGGT CAGTGTTGGG CTGCAAACCA GTGACATTAC TGAGTACCTC 
AGAAAGCTCA GTAAGACTGG AGTTCCTGAT GGAATTATCC AGTTTATTAA GCTGTGCACT 
GTCAGCTATG GGAAGGTCAA GCTGGTCCTC AAGCACAACA GGTACTTTGT TGAAAGTTCC 
CACCCTGATG TTATCCAGCA TCTTCTTCAA GACCCAGTGA TCCGGGAATG TCGCTTGAGG 
AACGCCGAGG GCGAGGCCAC CGAACTCATC ACAGAGACTT TTACAAGCAA ATCTGCTATT 
TCTAAGACGG CGGCGGAAGG CAGTGGCGGG CCTTCTACTT CGCAGGGGGT AGATGCACAG 
GCCACGTCTG ACATACCCAA AGACCTGTTT GATTTTTATG AGCAAATGGA CAAGGATGAG 
GAGGAGGAGG AAGAGACACA GACAGTGTCC TTTGAAGTTA AGCAGGAAAT GATCGAGGAG 
CTGCAGAAGC GCTGCATCTG CTTAGAGTAC CCGCTGCTGG CAGAGTATGA CTTCCGGAAT 
GACACTCTCA ATCCTGACAT CAACATTGAC CTGAAGCCCA CAGCCGTACT CAGACCCTAT 
CAGGAGAAGA GCCTGCGGAA GATGTTTGGG AACGGACGAG CACGCTCAGG AGTCATTGTT 
CTTCCCTGTG GTGCTGGGAA GTCCCTGGTC GGCGTGACTG CCGCATGCAC TGTCAGAAAG 
CGCTGTCTCG TCCTGGGCAA CTCGGCTGTG TCTGTGGAGC AGTGGAAAGC CCAGTTTAAG 
ATGTGGTCAA CCATCGATGA CAGCCAGATC TGCCGCTTCA CCTCAGATGC CAAGGACAAG 
CCCATCGGCT GCTCCGTTGC CATTAGCACT TACTCTATGC TGGGCCACAC CACCAAAAGG 
TCCTGGGAAG CTGAGAGAGT CATGGAGTGG CTCAAAACCC AGGAGTGGGG CCTCATGATC 
CTTGACGAGG TGCACACCAT TCCAGCCAGG ATGTTCCGGC GGGTTCTGAC TATTGTGCAG 
GCGCACTGTA AGCTTGGTTT GACTGCAACC CTTGTCCGGG AAGATGACAA AATTGTTGAC 
TTAAATTTCC TGATTGGGCC CAAGCTCTAT GAAGCCAACT GGATGGAGCT GCAGAACAAT 
GGGTACATCG CTAAAGTCCA GTGTGCTGAG GTTTGGTGCC CGATGTCTCC CGAGTTCTAC 
CGAGAGTATG TGGCAATCAA AACAAAGAAA CGGATCCTGT TGTACACCAT GAATCCCAAC 
AAATTCAGAG CCTGCCAGTT TCTGATCAAG TTTCATGAAA GGAGGAATGA CAAGATTATT 
GTCTTTGCTG ACAACGTATT TGCCTTGAAG GAATATGCCA TTCGGCTGAA CAAACCTTAT 
ATCTACGGGC CCACGTCCCA GGGAGAACGT ATGCAGATTC TCCAGAACTT CAAGCACAAC 
CCCAAAATCA ACACCATCTT CATCTCCAAG GTTGGTGACA CATCCTTTGA TCTGCCAGAA 
GCCAATGTCC TCATTCAGAT CTCTTCCCAT GGTGGCTCCC GACGGCAGGA AGCCCAGAGA 
CTGGGACGGG TACTCAGAGC CAAGAAAGGG ATGGTCGCAG AGGAATACAA TGCCTTTTTC 
TACTCCCTGG TATCTCAGGA CACACAGGAA ATGGCTTATT CTACCAAGCG ACAGAGATTC 
CTAGTGGATC AAGGTTACAG CTTTAAGGTA ATCACAAAGC TAGCTGGCAT GGAGGAAGAG 
GAGCTGGCGT TCTCCACCAA AGAGGAGCAG CAGCAGCTCC TGCAGAAGGT GCTGGCAGCC 
ACCGACTTGG ATGCAGAGGA GGAAGTGGTG GCTGGAGAGT TTGGCTCTAG ATCTGGCCAG 
GCATCCCGAC GCTGTGGCAC CATGAGCTCC CTGTCAGGCG CAGACGACAC CGTGTACATG 
GAGTACCACT CCTCCCGAAG CAAGGCCTCC AGCAAGCACG TGCACCCGCT TTTCAAACGC 
TTCAGGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ercc3 ( NM_133658.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598419.1 Mus musculus excision repair cross-complementing rodent repair deficiency, complementation group 3 (Ercc3), mRNA.

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CloneID	OMu22187
Clone ID Related Accession (Same CDS sequence)	NM_133658.1 , NM_133658.2
Accession Version	NM_133658.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	general transcription and DNA repair factor IIH helicase subunit XPB
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY273530.1, S71186.1 and BC022956.1. On Jul 17, 2018 this sequence version replaced NM_133658.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK151188.1, AK152347.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCAAAA GAGATCGAGT GGACCGCGAC AAAAAGAAAT CCAAGAAGAG GCAGTATGAA 
GAGGAAGAGG AAGACGAAGA CGACATTCCT GGGAACGAGT CTCAGGAGGC GGTGCCCTCC 
GCCGCTGGGA AACAGGTGGA CGAGTCCAGC ACCAAAGTGG ATGAGTATGG AGCAAAGGAC 
TACAGACAGC AGATGCCACT AAAGGGTGAC CATACCTCCA GGCCCCTCTG GGTGGCTCCT 
GATGGCCACA TTTTCTTGGA AGCCTTCTCT CCAGTTTACA AATATGCCCA AGACTTCCTG 
GTGGCAATTG CAGAGCCGGT GTGCCGGCCT ACTCATGTAC ATGAATACAA GCTAACCGCC 
TACTCCCTCT ATGCAGCGGT CAGTGTTGGG CTGCAAACCA GTGACATTAC TGAGTACCTC 
AGAAAGCTCA GTAAGACTGG AGTTCCTGAT GGAATTATCC AGTTTATTAA GCTGTGCACT 
GTCAGCTATG GGAAGGTCAA GCTGGTCCTC AAGCACAACA GGTACTTTGT TGAAAGTTCC 
CACCCTGATG TTATCCAGCA TCTTCTTCAA GACCCAGTGA TCCGGGAATG TCGCTTGAGG 
AACGCCGAGG GCGAGGCCAC CGAACTCATC ACAGAGACTT TTACAAGCAA ATCTGCTATT 
TCTAAGACGG CGGCGGAAGG CAGTGGCGGG CCTTCTACTT CGCAGGGGGT AGATGCACAG 
GCCACGTCTG ACATACCCAA AGACCTGTTT GATTTTTATG AGCAAATGGA CAAGGATGAG 
GAGGAGGAGG AAGAGACACA GACAGTGTCC TTTGAAGTTA AGCAGGAAAT GATCGAGGAG 
CTGCAGAAGC GCTGCATCTG CTTAGAGTAC CCGCTGCTGG CAGAGTATGA CTTCCGGAAT 
GACACTCTCA ATCCTGACAT CAACATTGAC CTGAAGCCCA CAGCCGTACT CAGACCCTAT 
CAGGAGAAGA GCCTGCGGAA GATGTTTGGG AACGGACGAG CACGCTCAGG AGTCATTGTT 
CTTCCCTGTG GTGCTGGGAA GTCCCTGGTC GGCGTGACTG CCGCATGCAC TGTCAGAAAG 
CGCTGTCTCG TCCTGGGCAA CTCGGCTGTG TCTGTGGAGC AGTGGAAAGC CCAGTTTAAG 
ATGTGGTCAA CCATCGATGA CAGCCAGATC TGCCGCTTCA CCTCAGATGC CAAGGACAAG 
CCCATCGGCT GCTCCGTTGC CATTAGCACT TACTCTATGC TGGGCCACAC CACCAAAAGG 
TCCTGGGAAG CTGAGAGAGT CATGGAGTGG CTCAAAACCC AGGAGTGGGG CCTCATGATC 
CTTGACGAGG TGCACACCAT TCCAGCCAGG ATGTTCCGGC GGGTTCTGAC TATTGTGCAG 
GCGCACTGTA AGCTTGGTTT GACTGCAACC CTTGTCCGGG AAGATGACAA AATTGTTGAC 
TTAAATTTCC TGATTGGGCC CAAGCTCTAT GAAGCCAACT GGATGGAGCT GCAGAACAAT 
GGGTACATCG CTAAAGTCCA GTGTGCTGAG GTTTGGTGCC CGATGTCTCC CGAGTTCTAC 
CGAGAGTATG TGGCAATCAA AACAAAGAAA CGGATCCTGT TGTACACCAT GAATCCCAAC 
AAATTCAGAG CCTGCCAGTT TCTGATCAAG TTTCATGAAA GGAGGAATGA CAAGATTATT 
GTCTTTGCTG ACAACGTATT TGCCTTGAAG GAATATGCCA TTCGGCTGAA CAAACCTTAT 
ATCTACGGGC CCACGTCCCA GGGAGAACGT ATGCAGATTC TCCAGAACTT CAAGCACAAC 
CCCAAAATCA ACACCATCTT CATCTCCAAG GTTGGTGACA CATCCTTTGA TCTGCCAGAA 
GCCAATGTCC TCATTCAGAT CTCTTCCCAT GGTGGCTCCC GACGGCAGGA AGCCCAGAGA 
CTGGGACGGG TACTCAGAGC CAAGAAAGGG ATGGTCGCAG AGGAATACAA TGCCTTTTTC 
TACTCCCTGG TATCTCAGGA CACACAGGAA ATGGCTTATT CTACCAAGCG ACAGAGATTC 
CTAGTGGATC AAGGTTACAG CTTTAAGGTA ATCACAAAGC TAGCTGGCAT GGAGGAAGAG 
GAGCTGGCGT TCTCCACCAA AGAGGAGCAG CAGCAGCTCC TGCAGAAGGT GCTGGCAGCC 
ACCGACTTGG ATGCAGAGGA GGAAGTGGTG GCTGGAGAGT TTGGCTCTAG ATCTGGCCAG 
GCATCCCGAC GCTGTGGCAC CATGAGCTCC CTGTCAGGCG CAGACGACAC CGTGTACATG 
GAGTACCACT CCTCCCGAAG CAAGGCCTCC AGCAAGCACG TGCACCCGCT TTTCAAACGC 
TTCAGGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598419.1
CDS	68..2419
Translation	MGKRDRVDRDKKKSKKRQYEEEEEDEDDIPGNESQEAVPSAAGKQVDESSTKVDEYGAKDYRQQMPLKGDHTSRPLWVAPDGHIFLEAFSPVYKYAQDFLVAIAEPVCRPTHVHEYKLTAYSLYAAVSVGLQTSDITEYLRKLSKTGVPDGIIQFIKLCTVSYGKVKLVLKHNRYFVESSHPDVIQHLLQDPVIRECRLRNAEGEATELITETFTSKSAISKTAAEGSGGPSTSQGVDAQATSDIPKDLFDFYEQMDKDEEEEEETQTVSFEVKQEMIEELQKRCICLEYPLLAEYDFRNDTLNPDINIDLKPTAVLRPYQEKSLRKMFGNGRARSGVIVLPCGAGKSLVGVTAACTVRKRCLVLGNSAVSVEQWKAQFKMWSTIDDSQICRFTSDAKDKPIGCSVAISTYSMLGHTTKRSWEAERVMEWLKTQEWGLMILDEVHTIPARMFRRVLTIVQAHCKLGLTATLVREDDKIVDLNFLIGPKLYEANWMELQNNGYIAKVQCAEVWCPMSPEFYREYVAIKTKKRILLYTMNPNKFRACQFLIKFHERRNDKIIVFADNVFALKEYAIRLNKPYIYGPTSQGERMQILQNFKHNPKINTIFISKVGDTSFDLPEANVLIQISSHGGSRRQEAQRLGRVLRAKKGMVAEEYNAFFYSLVSQDTQEMAYSTKRQRFLVDQGYSFKVITKLAGMEEEELAFSTKEEQQQLLQKVLAATDLDAEEEVVAGEFGSRSGQASRRCGTMSSLSGADDTVYMEYHSSRSKASSKHVHPLFKRFRK

Target ORF information:

RefSeq Version	NM_133658.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus excision repair cross-complementing rodent repair deficiency, complementation group 3 (Ercc3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133658.2

ORF Insert Sequence:

ATGGGCAAAA GAGATCGAGT GGACCGCGAC AAAAAGAAAT CCAAGAAGAG GCAGTATGAA 
GAGGAAGAGG AAGACGAAGA CGACATTCCT GGGAACGAGT CTCAGGAGGC GGTGCCCTCC 
GCCGCTGGGA AACAGGTGGA CGAGTCCAGC ACCAAAGTGG ATGAGTATGG AGCAAAGGAC 
TACAGACAGC AGATGCCACT AAAGGGTGAC CATACCTCCA GGCCCCTCTG GGTGGCTCCT 
GATGGCCACA TTTTCTTGGA AGCCTTCTCT CCAGTTTACA AATATGCCCA AGACTTCCTG 
GTGGCAATTG CAGAGCCGGT GTGCCGGCCT ACTCATGTAC ATGAATACAA GCTAACCGCC 
TACTCCCTCT ATGCAGCGGT CAGTGTTGGG CTGCAAACCA GTGACATTAC TGAGTACCTC 
AGAAAGCTCA GTAAGACTGG AGTTCCTGAT GGAATTATCC AGTTTATTAA GCTGTGCACT 
GTCAGCTATG GGAAGGTCAA GCTGGTCCTC AAGCACAACA GGTACTTTGT TGAAAGTTCC 
CACCCTGATG TTATCCAGCA TCTTCTTCAA GACCCAGTGA TCCGGGAATG TCGCTTGAGG 
AACGCCGAGG GCGAGGCCAC CGAACTCATC ACAGAGACTT TTACAAGCAA ATCTGCTATT 
TCTAAGACGG CGGCGGAAGG CAGTGGCGGG CCTTCTACTT CGCAGGGGGT AGATGCACAG 
GCCACGTCTG ACATACCCAA AGACCTGTTT GATTTTTATG AGCAAATGGA CAAGGATGAG 
GAGGAGGAGG AAGAGACACA GACAGTGTCC TTTGAAGTTA AGCAGGAAAT GATCGAGGAG 
CTGCAGAAGC GCTGCATCTG CTTAGAGTAC CCGCTGCTGG CAGAGTATGA CTTCCGGAAT 
GACACTCTCA ATCCTGACAT CAACATTGAC CTGAAGCCCA CAGCCGTACT CAGACCCTAT 
CAGGAGAAGA GCCTGCGGAA GATGTTTGGG AACGGACGAG CACGCTCAGG AGTCATTGTT 
CTTCCCTGTG GTGCTGGGAA GTCCCTGGTC GGCGTGACTG CCGCATGCAC TGTCAGAAAG 
CGCTGTCTCG TCCTGGGCAA CTCGGCTGTG TCTGTGGAGC AGTGGAAAGC CCAGTTTAAG 
ATGTGGTCAA CCATCGATGA CAGCCAGATC TGCCGCTTCA CCTCAGATGC CAAGGACAAG 
CCCATCGGCT GCTCCGTTGC CATTAGCACT TACTCTATGC TGGGCCACAC CACCAAAAGG 
TCCTGGGAAG CTGAGAGAGT CATGGAGTGG CTCAAAACCC AGGAGTGGGG CCTCATGATC 
CTTGACGAGG TGCACACCAT TCCAGCCAGG ATGTTCCGGC GGGTTCTGAC TATTGTGCAG 
GCGCACTGTA AGCTTGGTTT GACTGCAACC CTTGTCCGGG AAGATGACAA AATTGTTGAC 
TTAAATTTCC TGATTGGGCC CAAGCTCTAT GAAGCCAACT GGATGGAGCT GCAGAACAAT 
GGGTACATCG CTAAAGTCCA GTGTGCTGAG GTTTGGTGCC CGATGTCTCC CGAGTTCTAC 
CGAGAGTATG TGGCAATCAA AACAAAGAAA CGGATCCTGT TGTACACCAT GAATCCCAAC 
AAATTCAGAG CCTGCCAGTT TCTGATCAAG TTTCATGAAA GGAGGAATGA CAAGATTATT 
GTCTTTGCTG ACAACGTATT TGCCTTGAAG GAATATGCCA TTCGGCTGAA CAAACCTTAT 
ATCTACGGGC CCACGTCCCA GGGAGAACGT ATGCAGATTC TCCAGAACTT CAAGCACAAC 
CCCAAAATCA ACACCATCTT CATCTCCAAG GTTGGTGACA CATCCTTTGA TCTGCCAGAA 
GCCAATGTCC TCATTCAGAT CTCTTCCCAT GGTGGCTCCC GACGGCAGGA AGCCCAGAGA 
CTGGGACGGG TACTCAGAGC CAAGAAAGGG ATGGTCGCAG AGGAATACAA TGCCTTTTTC 
TACTCCCTGG TATCTCAGGA CACACAGGAA ATGGCTTATT CTACCAAGCG ACAGAGATTC 
CTAGTGGATC AAGGTTACAG CTTTAAGGTA ATCACAAAGC TAGCTGGCAT GGAGGAAGAG 
GAGCTGGCGT TCTCCACCAA AGAGGAGCAG CAGCAGCTCC TGCAGAAGGT GCTGGCAGCC 
ACCGACTTGG ATGCAGAGGA GGAAGTGGTG GCTGGAGAGT TTGGCTCTAG ATCTGGCCAG 
GCATCCCGAC GCTGTGGCAC CATGAGCTCC CTGTCAGGCG CAGACGACAC CGTGTACATG 
GAGTACCACT CCTCCCGAAG CAAGGCCTCC AGCAAGCACG TGCACCCGCT TTTCAAACGC 
TTCAGGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

ELL, a novel TFIIH partner, is involved in transcription restart after DNA repair.
Proceedings of the National Academy of Sciences of the United States of America110(44)17927-32(2013 Oct)
Mourgues S,Gautier V,Lagarou A,Bordier C,Mourcet A,Slingerland J,Kaddoum L,Coin F,Vermeulen W,Gonzales de Peredo A,Monsarrat B,Mari PO,Giglia-Mari G

Mutations in TFIIH causing trichothiodystrophy are responsible for defects in ribosomal RNA production and processing.
Human molecular genetics22(14)2881-93(2013 Jul)
Nonnekens J,Perez-Fernandez J,Theil AF,Gadal O,Bonnart C,Giglia-Mari G

Generation of DNA single-strand displacement by compromised nucleotide excision repair.
The EMBO journal31(17)3550-63(2012 Aug)
Godon C,Mourgues S,Nonnekens J,Mourcet A,Coin F,Vermeulen W,Mari PO,Giglia-Mari G

Defective transcription initiation causes postnatal growth failure in a mouse model of nucleotide excision repair (NER) progeria.
Proceedings of the National Academy of Sciences of the United States of America109(8)2995-3000(2012 Feb)
Kamileri I,Karakasilioti I,Sideri A,Kosteas T,Tatarakis A,Talianidis I,Garinis GA

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Ercc3 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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