Epas1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Epas1
Entrez Gene ID	13819
Full Name	endothelial PAS domain protein 1
Synonyms	HIF-2alpha,HIF2A,HLF,HRF,MOP2,bHLHe73
General protein information	Preferred Names endothelial PAS domain protein 1 Names endothelial PAS domain-containing protein 1 EPAS-1 HIF-1-alpha-like factor HIF-2-alpha HIF-related factor HIF1 alpha-like factor HIF1alpha-like factor HIF2-alpha HLF (HIF1alpha-like factor) Hif like protein hypoxia inducible transcription factor 2alpha hypoxia-inducible factor 2-alpha mHLF
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 E4

mRNA and Protein(s)

mRNA	Protein	Name
NM_010137.3	NP_034267.3	endothelial PAS domain-containing protein 1
XM_030249471.1	XP_030105331.1	endothelial PAS domain-containing protein 1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	EPAS1	XP_005626137.1
Rattus norvegicus (Norway rat)	Epas1	NP_075578.1
	EPAS1	XP_001112947.2
Mus musculus (house mouse)	Epas1	NP_034267.3
Pan troglodytes (chimpanzee)	EPAS1	XP_001147219.1
Gallus gallus (chicken)	EPAS1	NP_990138.1
Danio rerio (zebrafish)	epas1b	NP_001034895.1
Xenopus tropicalis (tropical clawed frog)	epas1	NP_001005647.1
Homo sapiens (human)	EPAS1	NP_001421.2
Bos taurus (cattle)	EPAS1	NP_777150.1

Gene Ontology

The following Epas1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Epas1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22704	NM_010137.3 Latest version!	Mus musculus endothelial PAS domain protein 1 (Epas1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$517.30 $739.00
OMu87080	XM_030249471.1 Latest version!	Mus musculus endothelial PAS domain protein 1 (Epas1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Epas1 ( NM_010137.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034267.3 Mus musculus endothelial PAS domain protein 1 (Epas1), mRNA.

Price & Availability↑

CloneID	OMu22704
Clone ID Related Accession (Same CDS sequence)	NM_010137.3
Accession Version	NM_010137.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	endothelial PAS domain-containing protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK136911.1, AK141185.1, U81983.1, D89787.1 and AC096777.1. On Apr 8, 2008 this sequence version replaced NM_010137.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK136911.1, SRR1660811.18563.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACAGCTG ACAAGGAGAA AAAAAGGAGC AGCTCAGAGC TGAGGAAGGA GAAATCCCGT 
GATGCCGCGA GGTGCCGGCG CAGCAAGGAG ACGGAGGTCT TCTATGAGTT GGCTCATGAG 
TTGCCCCTGC CTCACAGTGT GAGCTCCCAC CTGGACAAAG CCTCCATCAT GCGCCTGGCC 
ATCAGCTTCC TTCGGACACA TAAGCTCCTG TCCTCAGTCT GCTCTGAAAA TGAATCTGAA 
GCTGAGGCCG ACCAGCAAAT GGATAACTTG TACCTGAAAG CCTTGGAGGG TTTCATTGCT 
GTGGTGACCC AAGACGGTGA CATGATCTTT CTGTCGGAAA ACATCAGCAA GTTCATGGGA 
CTTACTCAGG TAGAACTAAC AGGACACAGC ATCTTTGACT TCACTCATCC TTGCGACCAT 
GAGGAGATCC GTGAGAACCT GACTCTCAAA AACGGCTCTG GTTTTGGGAA GAAGAGCAAA 
GACGTGTCCA CCGAGCGTGA CTTCTTCATG AGGATGAAGT GCACGGTCAC CAACAGAGGC 
CGGACTGTCA ACCTCAAGTC GGCCACCTGG AAGGTCCTGC ACTGCACCGG GCAAGTGAGA 
GTCTACAACA ACTGCCCCCC TCACAGTAGC CTCTGTGGCT CCAAGGAGCC CCTGCTGTCC 
TGCCTTATCA TCATGTGTGA GCCAATCCAG CACCCATCCC ACATGGACAT CCCCCTGGAC 
AGCAAGACTT TCCTGAGCCG CCACAGCATG GACATGAAGT TCACCTACTG TGACGACAGA 
ATCTTGGAAC TGATTGGTTA CCACCCCGAG GAGCTACTTG GACGCTCTGC CTATGAGTTC 
TACCATGCCC TGGATTCGGA GAACATGACC AAAAGTCACC AGAACTTGTG CACCAAGGGG 
CAGGTGGTAT CTGGCCAGTA CCGGATGCTA GCCAAACACG GAGGATATGT GTGGCTGGAG 
ACCCAGGGGA CGGTCATCTA CAACCCCCGC AACCTGCAGC CTCAGTGTAT CATGTGTGTC 
AACTATGTGC TGAGTGAGAT CGAGAAGAAC GACGTGGTGT TCTCCATGGA CCAGACCGAA 
TCCCTGTTCA AGCCACACCT GATGGCCATG AACAGCATCT TTGACAGCAG TGACGATGTG 
GCTGTAACTG AGAAGAGCAA CTACCTGTTC ACCAAACTGA AGGAGGAGCC CGAGGAACTG 
GCCCAGTTGG CCCCCACCCC AGGAGATGCC ATTATTTCTC TCGATTTCGG AAGCCAGAAC 
TTCGATGAAC CCTCAGCCTA TGGCAAGGCC ATCCTTCCCC CGGGCCAGCC ATGGGTCTCG 
GGGCTGAGGA GCCACAGTGC CCAGAGCGAG TCCGGGAGCC TGCCAGCCTT CACTGTGCCC 
CAGGCAGACA CCCCAGGGAA CACTACACCC AGTGCTTCAA GCAGCAGTAG CTGCTCCACG 
CCCAGCAGCC CTGAGGACTA CTATTCATCC TTGGAGAATC CCTTGAAGAT CGAAGTGATT 
GAGAAGCTTT TCGCCATGGA CACGGAGCCG AGGGACCCGG GCAGTACCCA GACGGACTTC 
AGTGAACTGG ATTTGGAGAC CTTGGCACCC TACATCCCTA TGGACGGCGA GGACTTCCAG 
CTGAGCCCCA TCTGCCCAGA GGAGCCGCTC ATGCCAGAGA GCCCCCAGCC CACCCCCCAG 
CACTGCTTCA GTACCATGAC CAGCATCTTC CAGCCGCTCA CCCCGGGGGC CACCCACGGC 
CCCTTCTTCC TCGATAAGTA CCCGCAGCAG TTGGAAAGCA GGAAGACAGA GTCTGAGCAC 
TGGCCCATGT CTTCCATCTT CTTTGATGCT GGGAGCAAAG GGTCCCTGTC TCCATGCTGT 
GGCCAGGCCA GCACCCCTCT CTCTTCTATG GGAGGCAGAT CCAACACGCA GTGGCCCCCG 
GATCCACCAT TACATTTCGG CCCTACTAAG TGGCCTGTGG GTGATCAGAG TGCTGAATCC 
CTGGGAGCCC TGCCGGTGGG GTCATCGCAG TTGGAACCTC CGAGCGCCCC GCCTCATGTC 
TCCATGTTCA AGATGAGGTC TGCAAAGGAC TTCGGGGCCC GAGGTCCATA CATGATGAGC 
CCAGCCATGA TCGCCCTGTC CAACAAGCTG AAGCTAAAGC GGCAGCTGGA GTATGAGGAG 
CAAGCCTTCC AAGACACAAG CGGGGGGGAC CCTCCAGGCA CCAGCAGTTC ACACTTGATG 
TGGAAACGTA TGAAGAGCCT CATGGGCGGG ACCTGTCCTT TGATGCCTGA CAAGACCATC 
AGTGCGAACA TGGCCCCCGA TGAATTCACC CAAAAATCTA TGAGAGGCCT GGGCCAGCCA 
CTGAGACACC TGCCACCTCC CCAGCCACCA TCTACCAGGA GCTCAGGGGA GAACGCCAAG 
ACTGGGTTCC CGCCACAGTG CTATGCCTCC CAGTTCCAGG ACTACGGTCC TCCAGGAGCT 
CAAAAGGTGT CAGGCGTGGC CAGTCGACTG CTGGGGCCAT CGTTCGAGCC TTACCTGTTG 
CCGGAACTGA CCAGATATGA CTGTGAGGTG AACGTGCCCG TGCCTGGAAG CTCCACACTC 
CTGCAGGGGA GAGACCTTCT CAGAGCTCTG GACCAGGCCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034267.3
CDS	420..3044
Translation	MTADKEKKRSSSELRKEKSRDAARCRRSKETEVFYELAHELPLPHSVSSHLDKASIMRLAISFLRTHKLLSSVCSENESEAEADQQMDNLYLKALEGFIAVVTQDGDMIFLSENISKFMGLTQVELTGHSIFDFTHPCDHEEIRENLTLKNGSGFGKKSKDVSTERDFFMRMKCTVTNRGRTVNLKSATWKVLHCTGQVRVYNNCPPHSSLCGSKEPLLSCLIIMCEPIQHPSHMDIPLDSKTFLSRHSMDMKFTYCDDRILELIGYHPEELLGRSAYEFYHALDSENMTKSHQNLCTKGQVVSGQYRMLAKHGGYVWLETQGTVIYNPRNLQPQCIMCVNYVLSEIEKNDVVFSMDQTESLFKPHLMAMNSIFDSSDDVAVTEKSNYLFTKLKEEPEELAQLAPTPGDAIISLDFGSQNFDEPSAYGKAILPPGQPWVSGLRSHSAQSESGSLPAFTVPQADTPGNTTPSASSSSSCSTPSSPEDYYSSLENPLKIEVIEKLFAMDTEPRDPGSTQTDFSELDLETLAPYIPMDGEDFQLSPICPEEPLMPESPQPTPQHCFSTMTSIFQPLTPGATHGPFFLDKYPQQLESRKTESEHWPMSSIFFDAGSKGSLSPCCGQASTPLSSMGGRSNTQWPPDPPLHFGPTKWPVGDQSAESLGALPVGSSQLEPPSAPPHVSMFKMRSAKDFGARGPYMMSPAMIALSNKLKLKRQLEYEEQAFQDTSGGDPPGTSSSHLMWKRMKSLMGGTCPLMPDKTISANMAPDEFTQKSMRGLGQPLRHLPPPQPPSTRSSGENAKTGFPPQCYASQFQDYGPPGAQKVSGVASRLLGPSFEPYLLPELTRYDCEVNVPVPGSSTLLQGRDLLRALDQAT

Target ORF information:

RefSeq Version	NM_010137.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus endothelial PAS domain protein 1 (Epas1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010137.3

ORF Insert Sequence:

ATGACAGCTG ACAAGGAGAA AAAAAGGAGC AGCTCAGAGC TGAGGAAGGA GAAATCCCGT 
GATGCCGCGA GGTGCCGGCG CAGCAAGGAG ACGGAGGTCT TCTATGAGTT GGCTCATGAG 
TTGCCCCTGC CTCACAGTGT GAGCTCCCAC CTGGACAAAG CCTCCATCAT GCGCCTGGCC 
ATCAGCTTCC TTCGGACACA TAAGCTCCTG TCCTCAGTCT GCTCTGAAAA TGAATCTGAA 
GCTGAGGCCG ACCAGCAAAT GGATAACTTG TACCTGAAAG CCTTGGAGGG TTTCATTGCT 
GTGGTGACCC AAGACGGTGA CATGATCTTT CTGTCGGAAA ACATCAGCAA GTTCATGGGA 
CTTACTCAGG TAGAACTAAC AGGACACAGC ATCTTTGACT TCACTCATCC TTGCGACCAT 
GAGGAGATCC GTGAGAACCT GACTCTCAAA AACGGCTCTG GTTTTGGGAA GAAGAGCAAA 
GACGTGTCCA CCGAGCGTGA CTTCTTCATG AGGATGAAGT GCACGGTCAC CAACAGAGGC 
CGGACTGTCA ACCTCAAGTC GGCCACCTGG AAGGTCCTGC ACTGCACCGG GCAAGTGAGA 
GTCTACAACA ACTGCCCCCC TCACAGTAGC CTCTGTGGCT CCAAGGAGCC CCTGCTGTCC 
TGCCTTATCA TCATGTGTGA GCCAATCCAG CACCCATCCC ACATGGACAT CCCCCTGGAC 
AGCAAGACTT TCCTGAGCCG CCACAGCATG GACATGAAGT TCACCTACTG TGACGACAGA 
ATCTTGGAAC TGATTGGTTA CCACCCCGAG GAGCTACTTG GACGCTCTGC CTATGAGTTC 
TACCATGCCC TGGATTCGGA GAACATGACC AAAAGTCACC AGAACTTGTG CACCAAGGGG 
CAGGTGGTAT CTGGCCAGTA CCGGATGCTA GCCAAACACG GAGGATATGT GTGGCTGGAG 
ACCCAGGGGA CGGTCATCTA CAACCCCCGC AACCTGCAGC CTCAGTGTAT CATGTGTGTC 
AACTATGTGC TGAGTGAGAT CGAGAAGAAC GACGTGGTGT TCTCCATGGA CCAGACCGAA 
TCCCTGTTCA AGCCACACCT GATGGCCATG AACAGCATCT TTGACAGCAG TGACGATGTG 
GCTGTAACTG AGAAGAGCAA CTACCTGTTC ACCAAACTGA AGGAGGAGCC CGAGGAACTG 
GCCCAGTTGG CCCCCACCCC AGGAGATGCC ATTATTTCTC TCGATTTCGG AAGCCAGAAC 
TTCGATGAAC CCTCAGCCTA TGGCAAGGCC ATCCTTCCCC CGGGCCAGCC ATGGGTCTCG 
GGGCTGAGGA GCCACAGTGC CCAGAGCGAG TCCGGGAGCC TGCCAGCCTT CACTGTGCCC 
CAGGCAGACA CCCCAGGGAA CACTACACCC AGTGCTTCAA GCAGCAGTAG CTGCTCCACG 
CCCAGCAGCC CTGAGGACTA CTATTCATCC TTGGAGAATC CCTTGAAGAT CGAAGTGATT 
GAGAAGCTTT TCGCCATGGA CACGGAGCCG AGGGACCCGG GCAGTACCCA GACGGACTTC 
AGTGAACTGG ATTTGGAGAC CTTGGCACCC TACATCCCTA TGGACGGCGA GGACTTCCAG 
CTGAGCCCCA TCTGCCCAGA GGAGCCGCTC ATGCCAGAGA GCCCCCAGCC CACCCCCCAG 
CACTGCTTCA GTACCATGAC CAGCATCTTC CAGCCGCTCA CCCCGGGGGC CACCCACGGC 
CCCTTCTTCC TCGATAAGTA CCCGCAGCAG TTGGAAAGCA GGAAGACAGA GTCTGAGCAC 
TGGCCCATGT CTTCCATCTT CTTTGATGCT GGGAGCAAAG GGTCCCTGTC TCCATGCTGT 
GGCCAGGCCA GCACCCCTCT CTCTTCTATG GGAGGCAGAT CCAACACGCA GTGGCCCCCG 
GATCCACCAT TACATTTCGG CCCTACTAAG TGGCCTGTGG GTGATCAGAG TGCTGAATCC 
CTGGGAGCCC TGCCGGTGGG GTCATCGCAG TTGGAACCTC CGAGCGCCCC GCCTCATGTC 
TCCATGTTCA AGATGAGGTC TGCAAAGGAC TTCGGGGCCC GAGGTCCATA CATGATGAGC 
CCAGCCATGA TCGCCCTGTC CAACAAGCTG AAGCTAAAGC GGCAGCTGGA GTATGAGGAG 
CAAGCCTTCC AAGACACAAG CGGGGGGGAC CCTCCAGGCA CCAGCAGTTC ACACTTGATG 
TGGAAACGTA TGAAGAGCCT CATGGGCGGG ACCTGTCCTT TGATGCCTGA CAAGACCATC 
AGTGCGAACA TGGCCCCCGA TGAATTCACC CAAAAATCTA TGAGAGGCCT GGGCCAGCCA 
CTGAGACACC TGCCACCTCC CCAGCCACCA TCTACCAGGA GCTCAGGGGA GAACGCCAAG 
ACTGGGTTCC CGCCACAGTG CTATGCCTCC CAGTTCCAGG ACTACGGTCC TCCAGGAGCT 
CAAAAGGTGT CAGGCGTGGC CAGTCGACTG CTGGGGCCAT CGTTCGAGCC TTACCTGTTG 
CCGGAACTGA CCAGATATGA CTGTGAGGTG AACGTGCCCG TGCCTGGAAG CTCCACACTC 
CTGCAGGGGA GAGACCTTCT CAGAGCTCTG GACCAGGCCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Epas1 ( XM_030249471.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105331.1 Mus musculus endothelial PAS domain protein 1 (Epas1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu87080
Clone ID Related Accession (Same CDS sequence)	XM_030249471.1
Accession Version	XM_030249471.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	endothelial PAS domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCCTGG CCATCAGCTT CCTTCGGACA CATAAGCTCC TGTCCTCAGT CTGCTCTGAA 
AATGAATCTG AAGCTGAGGC CGACCAGCAA ATGGATAACT TGTACCTGAA AGCCTTGGAG 
GGTTTCATTG CTGTGGTGAC CCAAGACGGT GACATGATCT TTCTGTCGGA AAACATCAGC 
AAGTTCATGG GACTTACTCA GGTAGAACTA ACAGGACACA GCATCTTTGA CTTCACTCAT 
CCTTGCGACC ATGAGGAGAT CCGTGAGAAC CTGACTCTCA AAAACGGCTC TGGTTTTGGG 
AAGAAGAGCA AAGACGTGTC CACCGAGCGT GACTTCTTCA TGAGGATGAA GTGCACGGTC 
ACCAACAGAG GCCGGACTGT CAACCTCAAG TCGGCCACCT GGAAGGTCCT GCACTGCACC 
GGGCAAGTGA GAGTCTACAA CAACTGCCCC CCTCACAGTA GCCTCTGTGG CTCCAAGGAG 
CCCCTGCTGT CCTGCCTTAT CATCATGTGT GAGCCAATCC AGCACCCATC CCACATGGAC 
ATCCCCCTGG ACAGCAAGAC TTTCCTGAGC CGCCACAGCA TGGACATGAA GTTCACCTAC 
TGTGACGACA GAATCTTGGA ACTGATTGGT TACCACCCCG AGGAGCTACT TGGACGCTCT 
GCCTATGAGT TCTACCATGC CCTGGATTCG GAGAACATGA CCAAAAGTCA CCAGAACTTG 
TGCACCAAGG GGCAGGTGGT ATCTGGCCAG TACCGGATGC TAGCCAAACA CGGAGGATAT 
GTGTGGCTGG AGACCCAGGG GACGGTCATC TACAACCCCC GCAACCTGCA GCCTCAGTGT 
ATCATGTGTG TCAACTATGT GCTGAGTGAG ATCGAGAAGA ACGACGTGGT GTTCTCCATG 
GACCAGACCG AATCCCTGTT CAAGCCACAC CTGATGGCCA TGAACAGCAT CTTTGACAGC 
AGTGACGATG TGGCTGTAAC TGAGAAGAGC AACTACCTGT TCACCAAACT GAAGGAGGAG 
CCCGAGGAAC TGGCCCAGTT GGCCCCCACC CCAGGAGATG CCATTATTTC TCTCGATTTC 
GGAAGCCAGA ACTTCGATGA ACCCTCAGCC TATGGCAAGG CCATCCTTCC CCCGGGCCAG 
CCATGGGTCT CGGGGCTGAG GAGCCACAGT GCCCAGAGCG AGTCCGGGAG CCTGCCAGCC 
TTCACTGTGC CCCAGGCAGA CACCCCAGGG AACACTACAC CCAGTGCTTC AAGCAGCAGT 
AGCTGCTCCA CGCCCAGCAG CCCTGAGGAC TACTATTCAT CCTTGGAGAA TCCCTTGAAG 
ATCGAAGTGA TTGAGAAGCT TTTCGCCATG GACACGGAGC CGAGGGACCC GGGCAGTACC 
CAGACGGACT TCAGTGAACT GGATTTGGAG ACCTTGGCAC CCTACATCCC TATGGACGGC 
GAGGACTTCC AGCTGAGCCC CATCTGCCCA GAGGAGCCGC TCATGCCAGA GAGCCCCCAG 
CCCACCCCCC AGCACTGCTT CAGTACCATG ACCAGCATCT TCCAGCCGCT CACCCCGGGG 
GCCACCCACG GCCCCTTCTT CCTCGATAAG TACCCGCAGC AGTTGGAAAG CAGGAAGACA 
GAGTCTGAGC ACTGGCCCAT GTCTTCCATC TTCTTTGATG CTGGGAGCAA AGGGTCCCTG 
TCTCCATGCT GTGGCCAGGC CAGCACCCCT CTCTCTTCTA TGGGAGGCAG ATCCAACACG 
CAGTGGCCCC CGGATCCACC ATTACATTTC GGCCCTACTA AGTGGCCTGT GGGTGATCAG 
AGTGCTGAAT CCCTGGGAGC CCTGCCGGTG GGGTCATCGC AGTTGGAACC TCCGAGCGCC 
CCGCCTCATG TCTCCATGTT CAAGATGAGG TCTGCAAAGG ACTTCGGGGC CCGAGGTCCA 
TACATGATGA GCCCAGCCAT GATCGCCCTG TCCAACAAGC TGAAGCTAAA GCGGCAGCTG 
GAGTATGAGG AGCAAGCCTT CCAAGACACA AGCGGGGGGG ACCCTCCAGG CACCAGCAGT 
TCACACTTGA TGTGGAAACG TATGAAGAGC CTCATGGGCG GGACCTGTCC TTTGATGCCT 
GACAAGACCA TCAGTGCGAA CATGGCCCCC GATGAATTCA CCCAAAAATC TATGAGAGGC 
CTGGGCCAGC CACTGAGACA CCTGCCACCT CCCCAGCCAC CATCTACCAG GAGCTCAGGG 
GAGAACGCCA AGACTGGGTT CCCGCCACAG TGCTATGCCT CCCAGTTCCA GGACTACGGT 
CCTCCAGGAG CTCAAAAGGT GTCAGGCGTG GCCAGTCGAC TGCTGGGGCC ATCGTTCGAG 
CCTTACCTGT TGCCGGAACT GACCAGATAT GACTGTGAGG TGAACGTGCC CGTGCCTGGA 
AGCTCCACAC TCCTGCAGGG GAGAGACCTT CTCAGAGCTC TGGACCAGGC CACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105331.1
CDS	244..2700
Translation	MRLAISFLRTHKLLSSVCSENESEAEADQQMDNLYLKALEGFIAVVTQDGDMIFLSENISKFMGLTQVELTGHSIFDFTHPCDHEEIRENLTLKNGSGFGKKSKDVSTERDFFMRMKCTVTNRGRTVNLKSATWKVLHCTGQVRVYNNCPPHSSLCGSKEPLLSCLIIMCEPIQHPSHMDIPLDSKTFLSRHSMDMKFTYCDDRILELIGYHPEELLGRSAYEFYHALDSENMTKSHQNLCTKGQVVSGQYRMLAKHGGYVWLETQGTVIYNPRNLQPQCIMCVNYVLSEIEKNDVVFSMDQTESLFKPHLMAMNSIFDSSDDVAVTEKSNYLFTKLKEEPEELAQLAPTPGDAIISLDFGSQNFDEPSAYGKAILPPGQPWVSGLRSHSAQSESGSLPAFTVPQADTPGNTTPSASSSSSCSTPSSPEDYYSSLENPLKIEVIEKLFAMDTEPRDPGSTQTDFSELDLETLAPYIPMDGEDFQLSPICPEEPLMPESPQPTPQHCFSTMTSIFQPLTPGATHGPFFLDKYPQQLESRKTESEHWPMSSIFFDAGSKGSLSPCCGQASTPLSSMGGRSNTQWPPDPPLHFGPTKWPVGDQSAESLGALPVGSSQLEPPSAPPHVSMFKMRSAKDFGARGPYMMSPAMIALSNKLKLKRQLEYEEQAFQDTSGGDPPGTSSSHLMWKRMKSLMGGTCPLMPDKTISANMAPDEFTQKSMRGLGQPLRHLPPPQPPSTRSSGENAKTGFPPQCYASQFQDYGPPGAQKVSGVASRLLGPSFEPYLLPELTRYDCEVNVPVPGSSTLLQGRDLLRALDQAT

Target ORF information:

RefSeq Version	XM_030249471.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus endothelial PAS domain protein 1 (Epas1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249471.1

ORF Insert Sequence:

ATGCGCCTGG CCATCAGCTT CCTTCGGACA CATAAGCTCC TGTCCTCAGT CTGCTCTGAA 
AATGAATCTG AAGCTGAGGC CGACCAGCAA ATGGATAACT TGTACCTGAA AGCCTTGGAG 
GGTTTCATTG CTGTGGTGAC CCAAGACGGT GACATGATCT TTCTGTCGGA AAACATCAGC 
AAGTTCATGG GACTTACTCA GGTAGAACTA ACAGGACACA GCATCTTTGA CTTCACTCAT 
CCTTGCGACC ATGAGGAGAT CCGTGAGAAC CTGACTCTCA AAAACGGCTC TGGTTTTGGG 
AAGAAGAGCA AAGACGTGTC CACCGAGCGT GACTTCTTCA TGAGGATGAA GTGCACGGTC 
ACCAACAGAG GCCGGACTGT CAACCTCAAG TCGGCCACCT GGAAGGTCCT GCACTGCACC 
GGGCAAGTGA GAGTCTACAA CAACTGCCCC CCTCACAGTA GCCTCTGTGG CTCCAAGGAG 
CCCCTGCTGT CCTGCCTTAT CATCATGTGT GAGCCAATCC AGCACCCATC CCACATGGAC 
ATCCCCCTGG ACAGCAAGAC TTTCCTGAGC CGCCACAGCA TGGACATGAA GTTCACCTAC 
TGTGACGACA GAATCTTGGA ACTGATTGGT TACCACCCCG AGGAGCTACT TGGACGCTCT 
GCCTATGAGT TCTACCATGC CCTGGATTCG GAGAACATGA CCAAAAGTCA CCAGAACTTG 
TGCACCAAGG GGCAGGTGGT ATCTGGCCAG TACCGGATGC TAGCCAAACA CGGAGGATAT 
GTGTGGCTGG AGACCCAGGG GACGGTCATC TACAACCCCC GCAACCTGCA GCCTCAGTGT 
ATCATGTGTG TCAACTATGT GCTGAGTGAG ATCGAGAAGA ACGACGTGGT GTTCTCCATG 
GACCAGACCG AATCCCTGTT CAAGCCACAC CTGATGGCCA TGAACAGCAT CTTTGACAGC 
AGTGACGATG TGGCTGTAAC TGAGAAGAGC AACTACCTGT TCACCAAACT GAAGGAGGAG 
CCCGAGGAAC TGGCCCAGTT GGCCCCCACC CCAGGAGATG CCATTATTTC TCTCGATTTC 
GGAAGCCAGA ACTTCGATGA ACCCTCAGCC TATGGCAAGG CCATCCTTCC CCCGGGCCAG 
CCATGGGTCT CGGGGCTGAG GAGCCACAGT GCCCAGAGCG AGTCCGGGAG CCTGCCAGCC 
TTCACTGTGC CCCAGGCAGA CACCCCAGGG AACACTACAC CCAGTGCTTC AAGCAGCAGT 
AGCTGCTCCA CGCCCAGCAG CCCTGAGGAC TACTATTCAT CCTTGGAGAA TCCCTTGAAG 
ATCGAAGTGA TTGAGAAGCT TTTCGCCATG GACACGGAGC CGAGGGACCC GGGCAGTACC 
CAGACGGACT TCAGTGAACT GGATTTGGAG ACCTTGGCAC CCTACATCCC TATGGACGGC 
GAGGACTTCC AGCTGAGCCC CATCTGCCCA GAGGAGCCGC TCATGCCAGA GAGCCCCCAG 
CCCACCCCCC AGCACTGCTT CAGTACCATG ACCAGCATCT TCCAGCCGCT CACCCCGGGG 
GCCACCCACG GCCCCTTCTT CCTCGATAAG TACCCGCAGC AGTTGGAAAG CAGGAAGACA 
GAGTCTGAGC ACTGGCCCAT GTCTTCCATC TTCTTTGATG CTGGGAGCAA AGGGTCCCTG 
TCTCCATGCT GTGGCCAGGC CAGCACCCCT CTCTCTTCTA TGGGAGGCAG ATCCAACACG 
CAGTGGCCCC CGGATCCACC ATTACATTTC GGCCCTACTA AGTGGCCTGT GGGTGATCAG 
AGTGCTGAAT CCCTGGGAGC CCTGCCGGTG GGGTCATCGC AGTTGGAACC TCCGAGCGCC 
CCGCCTCATG TCTCCATGTT CAAGATGAGG TCTGCAAAGG ACTTCGGGGC CCGAGGTCCA 
TACATGATGA GCCCAGCCAT GATCGCCCTG TCCAACAAGC TGAAGCTAAA GCGGCAGCTG 
GAGTATGAGG AGCAAGCCTT CCAAGACACA AGCGGGGGGG ACCCTCCAGG CACCAGCAGT 
TCACACTTGA TGTGGAAACG TATGAAGAGC CTCATGGGCG GGACCTGTCC TTTGATGCCT 
GACAAGACCA TCAGTGCGAA CATGGCCCCC GATGAATTCA CCCAAAAATC TATGAGAGGC 
CTGGGCCAGC CACTGAGACA CCTGCCACCT CCCCAGCCAC CATCTACCAG GAGCTCAGGG 
GAGAACGCCA AGACTGGGTT CCCGCCACAG TGCTATGCCT CCCAGTTCCA GGACTACGGT 
CCTCCAGGAG CTCAAAAGGT GTCAGGCGTG GCCAGTCGAC TGCTGGGGCC ATCGTTCGAG 
CCTTACCTGT TGCCGGAACT GACCAGATAT GACTGTGAGG TGAACGTGCC CGTGCCTGGA 
AGCTCCACAC TCCTGCAGGG GAGAGACCTT CTCAGAGCTC TGGACCAGGC CACCTGA