LEFTY1 cDNA ORF clone, Mus musculus(House mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	LEFTY1
Entrez Gene ID	13590
Full Name	left right determination factor 1
Synonyms	AI450052,Ebaf,Leftb,Stra3,Tgfb4,lefty,lefty-1
General protein information	Preferred Names left right determination factor 1 Names left-right determination factor 1 TGF-beta-4 left-right determination, factor B protein lefty-1 stimulated by retinoic acid gene 3 protein transforming growth factor beta-4
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: 1 Map Location: 1\|1 H4
Summary	This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in left-right asymmetry determination of organ systems during development. Mice lacking a functional copy of this gene exhibit embryonic lethality and defects in left-right patterning. [provided by RefSeq, Aug 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_010094.4	NP_034224.1	left-right determination factor 1 preproprotein
NM_010094.3	NP_034224.1	left-right determination factor 1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	LEFTY1	NP_066277.1
	LEFTY1	XP_001092988.1
Mus musculus (house mouse)	Lefty1	NP_034224.1
Rattus norvegicus (Norway rat)	Lefty1	NP_001102550.1
Pan troglodytes (chimpanzee)	LEFTY1	XP_001138066.1

Gene Ontology
Bibliography

Related articles in PubMed

Anti-inflammatory effects of Lefty-1 in renal tubulointerstitial inflammation via regulation of the NF-?B pathway.
Zhang L, Xu C, Hu W, Wu P, Qin C, Zhang J
International journal of molecular medicine41(3)1293-1304(2018 Mar)

Both Nodal signalling and stochasticity select for prospective distal visceral endoderm in mouse embryos.
Takaoka K, Nishimura H, Hamada H
Nature communications8(1)1492(2017 Nov)

Zfp281 is essential for mouse epiblast maturation through transcriptional and epigenetic control of Nodal signaling.
Huang X, Balmer S, Yang F, Fidalgo M, Li D, Guallar D, Hadjantonakis AK, Wang J
eLife6(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results show that TET-mediated oxidation of 5-methylcytosine modulates Lefty-Nodal signalling by promoting demethylation in opposition to methylation by DNMT3A and DNMT3B; findings reveal a fundamental epigenetic mechanism featuring dynamic DNA methylation and demethylation crucial to regulation of key signalling pathways in early body plan formation
Title: TET-mediated DNA demethylation controls gastrulation by regulating Lefty-Nodal signalling.

exogenous lefty1 administered to mice restored the endogenous expression levels of lefty1. The present study demonstrated that lefty1 attenuated renal interstitial injury by inhibiting the Smaddependent TGFb1 signaling pathway
Title: Lefty1 alleviates renal tubulointerstitial injury in mice with unilateral ureteral obstruction.

The expression of lefty in RCC cells was lower than that in adjacent non-tumor cells, which may result in the overexpression of nodal, thereby promoting the growth of RCC.
Title: Nodal activates smad and extracellular signal-regulated kinases 1/2 pathways promoting renal cell carcinoma proliferation.

Lefty-1 may prevent TGFB1-induced fibroblast-myofibroblast transdifferentiation in part via modulation of Smad3, JNK-3, and BMP-5 signaling.
Title: Lefty-1 alleviates TGF-?1-induced fibroblast-myofibroblast transdifferentiation in NRK-49F cells.

Studies indicate that the pair of Nodal and Lefty (Lefty1 and Lefty2) has a conserved role in left-right asymmetry.
Title: TGF? signaling in establishing left-right asymmetry.

The following LEFTY1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LEFTY1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu05512	NM_010094.4 Latest version!	Mus musculus left right determination factor 1 (Lefty1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
View Old Accession Versions >>
OMu05512	NM_010094.3	Mus musculus left right determination factor 1 (Lefty1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LEFTY1 ( NM_010094.4 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_034224.1 Mus musculus left right determination factor 1 (Lefty1), mRNA.

Price & Availability↑

CloneID	OMu05512
Clone ID Related Accession (Same CDS sequence)	NM_010094.4 , NM_010094.3
Accession Version	NM_010094.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	left-right determination factor 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC117673.18, AK131943.1 and AA216877.1. On Sep 1, 2016 this sequence version replaced NM_010094.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC050221.1, AK131943.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCATTCC TGTGGCTCTG CTGGGCACTC TGGGCACTGT CGCTGGTTAG CCTCAGGGAA 
GCCCTGACCG GAGAGCAGAT CCTGGGCAGC CTGCTGCAAC AGCTGCAGCT CGATCAACCG 
CCAGTCCTGG ACAAGGCTGA TGTGGAAGGG ATGGTCATCC CCTCGCACGT GAGGACTCAG 
TATGTGGCCC TGCTACAACA CAGCCATGCC AGCCGCTCCC GAGGCAAGAG GTTCAGCCAG 
AACCTTCGAG AGGTGGCAGG CAGGTTCCTG GTGTCAGAGA CCTCCACTCA CCTGCTAGTG 
TTCGGAATGG AGCAGCGGCT GCCGCCTAAC AGCGAGCTGG TGCAGGCTGT GCTGCGGCTG 
TTCCAGGAGC CTGTGCCCAG AACAGCTCTC CGGAGGCAAA AGAGGCTGTC CCCACACAGT 
GCCCGGGCTC GGGTCACCAT TGAATGGCTG CGCTTCCGCG ACGACGGCTC CAACCGCACT 
GCCCTTATCG ATTCTAGGCT CGTGTCCATC CACGAGAGCG GCTGGAAGGC CTTCGACGTG 
ACCGAGGCCG TGAACTTCTG GCAGCAGCTG AGCCGGCCGA GGCAGCCGCT GCTGCTCCAG 
GTGTCGGTGC AGAGGGAGCA TCTGGGGCCG GGAACCTGGA GCTCACACAA GTTGGTTCGT 
TTCGCGGCGC AGGGGACGCC GGATGGCAAG GGGCAGGGCG AGCCACAGCT GGAGCTGCAC 
ACGCTGGACC TCAAGGACTA TGGAGCTCAA GGCAATTGTG ACCCCGAGGC ACCAGTGACT 
GAAGGCACCC GATGCTGTCG CCAGGAGATG TACCTGGACC TGCAGGGGAT GAAGTGGGCC 
GAGAACTGGA TCCTAGAACC GCCAGGGTTC CTGACATATG AATGTGTGGG CAGCTGCCTG 
CAGCTACCGG AGTCCCTGAC CAGCAGGTGG CCATTTCTGG GGCCTCGGCA GTGTGTCGCC 
TCAGAGATGA CCTCCCTGCC CATGATTGTC AGCGTGAAGG AGGGAGGCAG GACCAGGCCT 
CAAGTGGTCA GCCTGCCCAA CATGAGGGTG CAGACCTGTA GCTGCGCCTC AGATGGGGCG 
CTCATACCCA GGAGGCTGCA GCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034224.1
CDS	89..1195
Translation	MPFLWLCWALWALSLVSLREALTGEQILGSLLQQLQLDQPPVLDKADVEGMVIPSHVRTQYVALLQHSHASRSRGKRFSQNLREVAGRFLVSETSTHLLVFGMEQRLPPNSELVQAVLRLFQEPVPRTALRRQKRLSPHSARARVTIEWLRFRDDGSNRTALIDSRLVSIHESGWKAFDVTEAVNFWQQLSRPRQPLLLQVSVQREHLGPGTWSSHKLVRFAAQGTPDGKGQGEPQLELHTLDLKDYGAQGNCDPEAPVTEGTRCCRQEMYLDLQGMKWAENWILEPPGFLTYECVGSCLQLPESLTSRWPFLGPRQCVASEMTSLPMIVSVKEGGRTRPQVVSLPNMRVQTCSCASDGALIPRRLQP

Target ORF information:

RefSeq Version	NM_010094.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus left right determination factor 1 (Lefty1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010094.4

ORF Insert Sequence:

ATGCCATTCC TGTGGCTCTG CTGGGCACTC TGGGCACTGT CGCTGGTTAG CCTCAGGGAA 
GCCCTGACCG GAGAGCAGAT CCTGGGCAGC CTGCTGCAAC AGCTGCAGCT CGATCAACCG 
CCAGTCCTGG ACAAGGCTGA TGTGGAAGGG ATGGTCATCC CCTCGCACGT GAGGACTCAG 
TATGTGGCCC TGCTACAACA CAGCCATGCC AGCCGCTCCC GAGGCAAGAG GTTCAGCCAG 
AACCTTCGAG AGGTGGCAGG CAGGTTCCTG GTGTCAGAGA CCTCCACTCA CCTGCTAGTG 
TTCGGAATGG AGCAGCGGCT GCCGCCTAAC AGCGAGCTGG TGCAGGCTGT GCTGCGGCTG 
TTCCAGGAGC CTGTGCCCAG AACAGCTCTC CGGAGGCAAA AGAGGCTGTC CCCACACAGT 
GCCCGGGCTC GGGTCACCAT TGAATGGCTG CGCTTCCGCG ACGACGGCTC CAACCGCACT 
GCCCTTATCG ATTCTAGGCT CGTGTCCATC CACGAGAGCG GCTGGAAGGC CTTCGACGTG 
ACCGAGGCCG TGAACTTCTG GCAGCAGCTG AGCCGGCCGA GGCAGCCGCT GCTGCTCCAG 
GTGTCGGTGC AGAGGGAGCA TCTGGGGCCG GGAACCTGGA GCTCACACAA GTTGGTTCGT 
TTCGCGGCGC AGGGGACGCC GGATGGCAAG GGGCAGGGCG AGCCACAGCT GGAGCTGCAC 
ACGCTGGACC TCAAGGACTA TGGAGCTCAA GGCAATTGTG ACCCCGAGGC ACCAGTGACT 
GAAGGCACCC GATGCTGTCG CCAGGAGATG TACCTGGACC TGCAGGGGAT GAAGTGGGCC 
GAGAACTGGA TCCTAGAACC GCCAGGGTTC CTGACATATG AATGTGTGGG CAGCTGCCTG 
CAGCTACCGG AGTCCCTGAC CAGCAGGTGG CCATTTCTGG GGCCTCGGCA GTGTGTCGCC 
TCAGAGATGA CCTCCCTGCC CATGATTGTC AGCGTGAAGG AGGGAGGCAG GACCAGGCCT 
CAAGTGGTCA GCCTGCCCAA CATGAGGGTG CAGACCTGTA GCTGCGCCTC AGATGGGGCG 
CTCATACCCA GGAGGCTGCA GCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LEFTY1 ( NM_010094.3 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_034224.1 Mus musculus left right determination factor 1 (Lefty1), mRNA.

Price & Availability↑

CloneID	OMu05512
Clone ID Related Accession (Same CDS sequence)	NM_010094.4 , NM_010094.3
Accession Version	NM_010094.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	left-right determination factor 1 precursor
Comment

ATGCCATTCC TGTGGCTCTG CTGGGCACTC TGGGCACTGT CGCTGGTTAG CCTCAGGGAA 
GCCCTGACCG GAGAGCAGAT CCTGGGCAGC CTGCTGCAAC AGCTGCAGCT CGATCAACCG 
CCAGTCCTGG ACAAGGCTGA TGTGGAAGGG ATGGTCATCC CCTCGCACGT GAGGACTCAG 
TATGTGGCCC TGCTACAACA CAGCCATGCC AGCCGCTCCC GAGGCAAGAG GTTCAGCCAG 
AACCTTCGAG AGGTGGCAGG CAGGTTCCTG GTGTCAGAGA CCTCCACTCA CCTGCTAGTG 
TTCGGAATGG AGCAGCGGCT GCCGCCTAAC AGCGAGCTGG TGCAGGCTGT GCTGCGGCTG 
TTCCAGGAGC CTGTGCCCAG AACAGCTCTC CGGAGGCAAA AGAGGCTGTC CCCACACAGT 
GCCCGGGCTC GGGTCACCAT TGAATGGCTG CGCTTCCGCG ACGACGGCTC CAACCGCACT 
GCCCTTATCG ATTCTAGGCT CGTGTCCATC CACGAGAGCG GCTGGAAGGC CTTCGACGTG 
ACCGAGGCCG TGAACTTCTG GCAGCAGCTG AGCCGGCCGA GGCAGCCGCT GCTGCTCCAG 
GTGTCGGTGC AGAGGGAGCA TCTGGGGCCG GGAACCTGGA GCTCACACAA GTTGGTTCGT 
TTCGCGGCGC AGGGGACGCC GGATGGCAAG GGGCAGGGCG AGCCACAGCT GGAGCTGCAC 
ACGCTGGACC TCAAGGACTA TGGAGCTCAA GGCAATTGTG ACCCCGAGGC ACCAGTGACT 
GAAGGCACCC GATGCTGTCG CCAGGAGATG TACCTGGACC TGCAGGGGAT GAAGTGGGCC 
GAGAACTGGA TCCTAGAACC GCCAGGGTTC CTGACATATG AATGTGTGGG CAGCTGCCTG 
CAGCTACCGG AGTCCCTGAC CAGCAGGTGG CCATTTCTGG GGCCTCGGCA GTGTGTCGCC 
TCAGAGATGA CCTCCCTGCC CATGATTGTC AGCGTGAAGG AGGGAGGCAG GACCAGGCCT 
CAAGTGGTCA GCCTGCCCAA CATGAGGGTG CAGACCTGTA GCTGCGCCTC AGATGGGGCG 
CTCATACCCA GGAGGCTGCA GCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034224.1
Translation	MPFLWLCWALWALSLVSLREALTGEQILGSLLQQLQLDQPPVLDKADVEGMVIPSHVRTQYVALLQHSHASRSRGKRFSQNLREVAGRFLVSETSTHLLVFGMEQRLPPNSELVQAVLRLFQEPVPRTALRRQKRLSPHSARARVTIEWLRFRDDGSNRTALIDSRLVSIHESGWKAFDVTEAVNFWQQLSRPRQPLLLQVSVQREHLGPGTWSSHKLVRFAAQGTPDGKGQGEPQLELHTLDLKDYGAQGNCDPEAPVTEGTRCCRQEMYLDLQGMKWAENWILEPPGFLTYECVGSCLQLPESLTSRWPFLGPRQCVASEMTSLPMIVSVKEGGRTRPQVVSLPNMRVQTCSCASDGALIPRRLQP

Target ORF information:

RefSeq Version	NM_010094.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus left right determination factor 1 (Lefty1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010094.3

ORF Insert Sequence:

ATGCCATTCC TGTGGCTCTG CTGGGCACTC TGGGCACTGT CGCTGGTTAG CCTCAGGGAA 
GCCCTGACCG GAGAGCAGAT CCTGGGCAGC CTGCTGCAAC AGCTGCAGCT CGATCAACCG 
CCAGTCCTGG ACAAGGCTGA TGTGGAAGGG ATGGTCATCC CCTCGCACGT GAGGACTCAG 
TATGTGGCCC TGCTACAACA CAGCCATGCC AGCCGCTCCC GAGGCAAGAG GTTCAGCCAG 
AACCTTCGAG AGGTGGCAGG CAGGTTCCTG GTGTCAGAGA CCTCCACTCA CCTGCTAGTG 
TTCGGAATGG AGCAGCGGCT GCCGCCTAAC AGCGAGCTGG TGCAGGCTGT GCTGCGGCTG 
TTCCAGGAGC CTGTGCCCAG AACAGCTCTC CGGAGGCAAA AGAGGCTGTC CCCACACAGT 
GCCCGGGCTC GGGTCACCAT TGAATGGCTG CGCTTCCGCG ACGACGGCTC CAACCGCACT 
GCCCTTATCG ATTCTAGGCT CGTGTCCATC CACGAGAGCG GCTGGAAGGC CTTCGACGTG 
ACCGAGGCCG TGAACTTCTG GCAGCAGCTG AGCCGGCCGA GGCAGCCGCT GCTGCTCCAG 
GTGTCGGTGC AGAGGGAGCA TCTGGGGCCG GGAACCTGGA GCTCACACAA GTTGGTTCGT 
TTCGCGGCGC AGGGGACGCC GGATGGCAAG GGGCAGGGCG AGCCACAGCT GGAGCTGCAC 
ACGCTGGACC TCAAGGACTA TGGAGCTCAA GGCAATTGTG ACCCCGAGGC ACCAGTGACT 
GAAGGCACCC GATGCTGTCG CCAGGAGATG TACCTGGACC TGCAGGGGAT GAAGTGGGCC 
GAGAACTGGA TCCTAGAACC GCCAGGGTTC CTGACATATG AATGTGTGGG CAGCTGCCTG 
CAGCTACCGG AGTCCCTGAC CAGCAGGTGG CCATTTCTGG GGCCTCGGCA GTGTGTCGCC 
TCAGAGATGA CCTCCCTGCC CATGATTGTC AGCGTGAAGG AGGGAGGCAG GACCAGGCCT 
CAAGTGGTCA GCCTGCCCAA CATGAGGGTG CAGACCTGTA GCTGCGCCTC AGATGGGGCG 
CTCATACCCA GGAGGCTGCA GCCATAG